CmaCh12G010860.1 (mRNA) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh12G010860.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Kunitz trypsin inhibitor
Location	Cma_Chr12 : 8584615 .. 8585619 (+)
Sequence length	913

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAAATGGATATCTCTTCAAATTGGCTATAAATGTCACTACACATTTGCATCCCATAACCCATAAGTTTTCGAACAAATTAATGATGTTGTGTAAGTCACTCGTCGCCTTTAGCTACCTATGTCTAGTCATGGCTGCGTCCTCTATCGCTCAAAGTCCTCCCGTGCTCGACATAGAGGGCCGACCTCTAGAGATCAACGTCGAGTACTTCATCAAGCCTGCCATCACTGACGTCGCTGGAAATCTCACCTTATTAACCCGCAAAGGCGATCAGTGCCCGTTTCATGTTGGTCAAGTACCACTTCGTTCACAAGAAATAGGTATTATTACATCACTATCGAACTCAAAAACTCAATTTAATCAGTGATACCATTAGAAAACTCTTGATTATGTTATGAATTCTTAACCATAGGTTTCGGGGTCACCTTTACACCCTACCTACCGAGTGAAGATATGATCAGAGAAGGGAGGGATTTGAAGGTTGTGTTTCAAGCATTTTCGATCTGCATAACGTCGACTGCATGGAGAGTCGGGGAGGCAGATCCTACCACTGGAAGGAGGTTTGTCGAGACTGGGGACGACGACACTGCCACCGGGTACTTTAGGATCGACAGGGACAACTTTGGTGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCTATTAGAGGGAGGCCAAGGTGTGGAAGGGCTGGAATTTTGATTGAGAAAGGCTTGAGGCTGTTGGCTTTGGATGGCTCAGCGTTTCCTTTTGAGTTTGTGAGAGCTTGAGACAAGACCATGCAATTTGTTGCAATTGCATTAAATTAAATAATACTCTTGTTAAGTATTTTAGTTTATTTGATTAAAAAAATAATTAATAAAGGAATTCCGACGTTTTAGATTTAGTTTCTATGTCATTTTTAGGGTAAGAGCTCGAGCCCACGGCCAGGAGATATTGTCCTATTTGGACTTTCGTTCAAGGTTTTAAAACGGGTCTCAAGATTATAAAAATGTTTCGTTCCCC

mRNA sequence

CAAAATGGATATCTCTTCAAATTGGCTATAAATGTCACTACACATTTGCATCCCATAACCCATAAGTTTTCGAACAAATTAATGATGTTGTGTAAGTCACTCGTCGCCTTTAGCTACCTATGTCTAGTCATGGCTGCGTCCTCTATCGCTCAAAGTCCTCCCGTGCTCGACATAGAGGGCCGACCTCTAGAGATCAACGTCGAGTACTTCATCAAGCCTGCCATCACTGACGTCGCTGGAAATCTCACCTTATTAACCCGCAAAGGCGATCAGTGCCCGTTTCATGTTGGTCAAGTACCACTTCGTTCACAAGAAATAGGTTTCGGGGTCACCTTTACACCCTACCTACCGAGTGAAGATATGATCAGAGAAGGGAGGGATTTGAAGGTTGTGTTTCAAGCATTTTCGATCTGCATAACGTCGACTGCATGGAGAGTCGGGGAGGCAGATCCTACCACTGGAAGGAGGTTTGTCGAGACTGGGGACGACGACACTGCCACCGGGTACTTTAGGATCGACAGGGACAACTTTGGTGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCTATTAGAGGGAGGCCAAGGTGTGGAAGGGCTGGAATTTTGATTGAGAAAGGCTTGAGGCTGTTGGCTTTGGATGGCTCAGCGTTTCCTTTTGAGTTTGTGAGAGCTTGAGACAAGACCATGCAATTTGTTGCAATTGCATTAAATTAAATAATACTCTTGTTAAGTATTTTAGTTTATTTGATTAAAAAAATAATTAATAAAGGAATTCCGACGTTTTAGATTTAGTTTCTATGTCATTTTTAGGGTAAGAGCTCGAGCCCACGGCCAGGAGATATTGTCCTATTTGGACTTTCGTTCAAGGTTTTAAAACGGGTCTCAAGATTATAAAAATGTTTCGTTCCCC

Coding sequence (CDS)

ATGATGTTGTGTAAGTCACTCGTCGCCTTTAGCTACCTATGTCTAGTCATGGCTGCGTCCTCTATCGCTCAAAGTCCTCCCGTGCTCGACATAGAGGGCCGACCTCTAGAGATCAACGTCGAGTACTTCATCAAGCCTGCCATCACTGACGTCGCTGGAAATCTCACCTTATTAACCCGCAAAGGCGATCAGTGCCCGTTTCATGTTGGTCAAGTACCACTTCGTTCACAAGAAATAGGTTTCGGGGTCACCTTTACACCCTACCTACCGAGTGAAGATATGATCAGAGAAGGGAGGGATTTGAAGGTTGTGTTTCAAGCATTTTCGATCTGCATAACGTCGACTGCATGGAGAGTCGGGGAGGCAGATCCTACCACTGGAAGGAGGTTTGTCGAGACTGGGGACGACGACACTGCCACCGGGTACTTTAGGATCGACAGGGACAACTTTGGTGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCTATTAGAGGGAGGCCAAGGTGTGGAAGGGCTGGAATTTTGATTGAGAAAGGCTTGAGGCTGTTGGCTTTGGATGGCTCAGCGTTTCCTTTTGAGTTTGTGAGAGCTTGA

Protein sequence

MMLCKSLVAFSYLCLVMAASSIAQSPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGEADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFVRA

Homology

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BLAST of CmaCh12G010860.1 vs. Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 92.4 bits (228), Expect = 5.8e-18
Identity = 71/203 (34.98%), Postives = 101/203 (49.75%), Query Frame = 1


Query: 6   SLVAFSYLCLVMAASSIAQSPPVLDIEGRPLEINVEYFIKPAITDVAGNLTL--LTRKGD 65
           +L+A +   L+ AA S     PVLDI+G  L     Y+I P + D  G LT+   T  G 
Sbjct: 16  ALLAAAANPLLSAADSAPN--PVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGT 75

Query: 66  -QCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSIC--ITSTAWRVG 125
             CP  V Q   +  +    + F P  P ED++R   DL + F AF  C   +ST WR+ 
Sbjct: 76  FVCPPRVVQTR-KEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLD 135

Query: 126 EADPTTGRRFVETG------DDDTATGYFRIDR-DNFGVYNIGWCPSDVPIRGRPRCGRA 185
           + D +TG+ FV  G        +T + +F+I+     G Y + +CP+ V    + +CG  
Sbjct: 136 KYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPT-VCGSCKVKCGDV 195

Query: 186 GILI-EKGLRLLALDGSAFPFEF 196
           GI I +KG R LAL    F FEF
Sbjct: 196 GIYIDQKGRRRLALSDKPFAFEF 214

[Close]

BLAST of CmaCh12G010860.1 vs. Swiss-Prot
Match: IAAS_ORYSJ (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 9.6e-13
Identity = 50/137 (36.50%), Postives = 69/137 (50.36%), Query Frame = 1


Query: 2   MLCKSLVAFSYLCLVMAASSIAQSPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRK 61
           ++  SL+A S+ C        A  PPV D EG  L  +  Y++ PA     G LT+  R 
Sbjct: 8   LILLSLLAISFSCS-------AAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRV 67

Query: 62  GDQCPFHVGQVPLRSQEIGFGVTFTPY----LPSEDMIREGRDLKVVFQAFSICITSTAW 121
              CP  V Q     ++ GF V FTP+     P +  IR   D+++ F A +IC+ ST W
Sbjct: 68  LP-CPLLVAQETDERRK-GFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEW 127

Query: 122 RVGEADPTTGRRFVETG 135
            VG+ +P TG R V TG
Sbjct: 128 HVGD-EPLTGARRVVTG 134

[Close]

BLAST of CmaCh12G010860.1 vs. Swiss-Prot
Match: IAAS_WHEAT (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.8e-12
Identity = 43/129 (33.33%), Postives = 68/129 (52.71%), Query Frame = 1


Query: 26  PPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRSQEIGFGVTF 85
           PPV D +G  L  +  Y++ PA     G LT+    G +CP  V Q     Q  G  V  
Sbjct: 3   PPVHDTDGNELRADANYYVLPANRAHGGGLTMAPGHGRRCPLFVSQ-EADGQRDGLPVRI 62

Query: 86  TPY--LPSEDMIREGRDLKVVFQAFSICITSTAWRVGEADPTTGRRFVETGD--DDTATG 145
            P+   PS+ +IR   D+++ F+A++ C+ ST W + +++  +GRR V TG   D + +G
Sbjct: 63  APHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHI-DSELVSGRRHVITGPVRDPSPSG 122

Query: 146 ---YFRIDR 148
               FRI++
Sbjct: 123 RENAFRIEK 129

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BLAST of CmaCh12G010860.1 vs. Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 5.0e-09
Identity = 48/164 (29.27%), Postives = 76/164 (46.34%), Query Frame = 1


Query: 7   LVAFSYLCLVMAASSIAQSPPVLDIEGRPLEINVEYFIKPAITDVAGN-LTLLTRKGDQC 66
           L AF+        ++ A SP VLD +G  L+  V+Y++  +I+   G  L L    G  C
Sbjct: 11  LFAFTSKSYFFGVANAANSP-VLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSC 70

Query: 67  PFHVGQVPLRSQ-EIGFGVTFTPYLPSEDMIREGRDLKVVFQAFS--ICITSTAWRVGEA 126
           P  V  V  RS  + G  V F+     +D++R   D+ + F      +C TST WR+   
Sbjct: 71  PEIV--VQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNY 130

Query: 127 DPTTGRRFVETG------DDDTATGYFRIDRDNFGVYNIGWCPS 161
           D + G+ +V T         +T   +F+I++     Y   +CPS
Sbjct: 131 DNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPS 171

[Close]

BLAST of CmaCh12G010860.1 vs. Swiss-Prot
Match: PIP21_PEA (Kunitz-type trypsin inhibitor-like 1 protein OS=Pisum sativum GN=PIP20-1 PE=1 SV=2)

HSP 1 Score: 61.2 bits (147), Expect = 1.4e-08
Identity = 60/208 (28.85%), Postives = 88/208 (42.31%), Query Frame = 1


Query: 3   LCKSLVAFSYLCLVMAASSIAQSPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKG 62
           L  S   F ++  +  A S      VLD  G P+   V+YFI PAI   AG    L R G
Sbjct: 7   LTLSFFLFVFITNLSLAFSNEDVEQVLDFNGNPIFPGVQYFILPAIRGPAGGGVRLGRTG 66

Query: 63  D-QCPFHVGQVPLRSQEIGFGVTFTPYLP--SEDMIREGRDLKVVFQAFSICITSTAWRV 122
           D  CP  V Q     QE+  G+     +P  S  +I  G  +++ +     C  S+ W V
Sbjct: 67  DLTCPVTVLQ---DRQEVKNGLPVKFVIPEISPGIIFTGTPIEIEYTKKPNCAKSSKWLV 126

Query: 123 GEADPTTGRRFVETGDDD------TATGYFRIDRDNFGV-YNIGWCPSDVPIRGRPRCGR 182
              D    +  V  G  +      T +G F+I++   G  Y +G+C     ++G P C  
Sbjct: 127 -FVDNVIQKACVGIGGPENYPGVQTLSGLFKIEKHESGFGYKLGFC-----VKGSPTCLD 186

Query: 183 AGIL--IEKGLRLLALDGSAFPFEFVRA 199
            G     E G RL   +  +F   F++A
Sbjct: 187 VGRFDNDEDGRRLNLTEHESFQVVFIQA 205

[Close]

BLAST of CmaCh12G010860.1 vs. TrEMBL
Match: A0A0A0LGB4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021000 PE=4 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 2.1e-83
Identity = 153/200 (76.50%), Postives = 169/200 (84.50%), Query Frame = 1


Query: 1   MMLCKSLVA-FSYLCLVMAASSIAQ--SPPVLDIEGRPLEINVEYFIKPAITDVAGNLTL 60
           M   KSL+A FS+LC  MA  S AQ   PPVLD EGRPL+ +VEYFIKPAITDVAGNLTL
Sbjct: 2   MRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 61

Query: 61  LTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAW 120
           +TRKGDQCPF+VGQVPL SQEIGFGVT TPYL  +D IREGRDLK VFQAF+ICIT T W
Sbjct: 62  ITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQW 121

Query: 121 RVGEADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILI 180
           +VGE DP +GRRFV+ G+D+TATGYFRID+ N GVYNIGWCPSDVP +GRPRCG AGILI
Sbjct: 122 KVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILI 181

Query: 181 EKGLRLLALDGSAFPFEFVR 198
           EKG+R LALDG AFPFEFVR
Sbjct: 182 EKGVRFLALDGPAFPFEFVR 201

[Close]

BLAST of CmaCh12G010860.1 vs. TrEMBL
Match: D1KFM4_9ROSI (Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus nigra PE=2 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 5.4e-55
Identity = 119/195 (61.03%), Postives = 134/195 (68.72%), Query Frame = 1


Query: 11  SYLCLVMAASSIAQ-----SPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQC 70
           S++ L MA  S+AQ     SPPVLD  G+PLE  VEY+I P ITDVAG LTL+ R G +C
Sbjct: 9   SFVWLTMAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRC 68

Query: 71  PFHVGQVPLRSQE-IGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGEADP 130
           PF+VGQ PL S E  G  V FTPY   E +IRE RDL V FQA +ICI STAWRVGE DP
Sbjct: 69  PFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDP 128

Query: 131 TTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSD-VPIRGRPRCGRAGILIEKGLRL 190
            TGRRF+ T  D +   YFRID +N GVYN  WCP++  P   RPRCG AGILIE   RL
Sbjct: 129 ETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKRL 188

Query: 191 LALDGSAFPFEFVRA 199
           LALDG AFPF F RA
Sbjct: 189 LALDGPAFPFVFTRA 199

[Close]

BLAST of CmaCh12G010860.1 vs. TrEMBL
Match: G0Z2L7_POPNI (Kunitz-type trypsin inhibitor C5 OS=Populus nigra PE=2 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 5.4e-55
Identity = 118/195 (60.51%), Postives = 134/195 (68.72%), Query Frame = 1


Query: 11  SYLCLVMAASSIAQ-----SPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQC 70
           S++ L MA  S+AQ     SPPVLD  G+PLE  VEY+I P ITD+AG LTL+ R G +C
Sbjct: 9   SFVWLTMAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDIAGGLTLVNRNGIRC 68

Query: 71  PFHVGQVPLRSQE-IGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGEADP 130
           PF+VGQ PL S E  G  V FTPY   E +IRE RDL V FQA +ICI STAWRVGE DP
Sbjct: 69  PFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDP 128

Query: 131 TTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSD-VPIRGRPRCGRAGILIEKGLRL 190
            TGRRF+ T  D +   YFRID +N GVYN  WCP++  P   RPRCG AGILIE   RL
Sbjct: 129 ETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPTCDRPRCGSAGILIEDDKRL 188

Query: 191 LALDGSAFPFEFVRA 199
           LALDG AFPF F RA
Sbjct: 189 LALDGPAFPFVFTRA 199

[Close]

BLAST of CmaCh12G010860.1 vs. TrEMBL
Match: D1KFM3_9ROSI (Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides PE=2 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 5.4e-55
Identity = 119/195 (61.03%), Postives = 134/195 (68.72%), Query Frame = 1


Query: 11  SYLCLVMAASSIAQ-----SPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQC 70
           S++ L MA  S+AQ     SPPVLD  G+PLE  VEY+I P ITDVAG LTL+ R G +C
Sbjct: 9   SFVWLTMAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRC 68

Query: 71  PFHVGQVPLRSQE-IGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGEADP 130
           PF+VGQ PL S E  G  V FTPY   E +IRE RDL V FQA +ICI STAWRVGE DP
Sbjct: 69  PFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDP 128

Query: 131 TTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSD-VPIRGRPRCGRAGILIEKGLRL 190
            TGRRF+ T  D +   YFRID +N GVYN  WCP++  P   RPRCG AGILIE   RL
Sbjct: 129 ETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKRL 188

Query: 191 LALDGSAFPFEFVRA 199
           LALDG AFPF F RA
Sbjct: 189 LALDGPAFPFVFTRA 199

[Close]

BLAST of CmaCh12G010860.1 vs. TrEMBL
Match: B9N8J2_POPTR (Truncated Kunitz trypsin inhibitor family protein OS=Populus trichocarpa GN=POPTR_0004s00220g PE=4 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 9.3e-55
Identity = 119/195 (61.03%), Postives = 134/195 (68.72%), Query Frame = 1


Query: 11  SYLCLVMAASSIAQ-----SPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQC 70
           S++ L MA  S+AQ     SPPVLD  G+PLE  VEY+I P ITDVAG LTL+ R G +C
Sbjct: 9   SFVWLTMAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRC 68

Query: 71  PFHVGQVPLRSQE-IGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGEADP 130
           PF+VGQ PL S E  G  V FTPY   E +IRE RDL V FQA +ICI STAWRVGE DP
Sbjct: 69  PFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQASTICIQSTAWRVGEEDP 128

Query: 131 TTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSD-VPIRGRPRCGRAGILIEKGLRL 190
            TGRRF+ T  D +   YFRID +N GVYN  WCP++  P   RPRCG AGILIE   RL
Sbjct: 129 ETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKRL 188

Query: 191 LALDGSAFPFEFVRA 199
           LALDG AFPF F RA
Sbjct: 189 LALDGPAFPFVFTRA 199

[Close]

BLAST of CmaCh12G010860.1 vs. TAIR10
Match: AT1G73260.1 (AT1G73260.1 kunitz trypsin inhibitor 1)

HSP 1 Score: 86.3 bits (212), Expect = 2.4e-17
Identity = 61/195 (31.28%), Postives = 90/195 (46.15%), Query Frame = 1


Query: 15  LVMAASSIAQSPP---VLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQ 74
           LV+A +++  S     V+DI+G  +  +  Y++ P I    G LTL  R G  CP+ + Q
Sbjct: 14  LVLALTAVLASNAYGAVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQ 73

Query: 75  VPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQA-FSICITSTAWRVGEADPTTGRRF 134
                 E G  V F+ +      + E ++L +      +ICI ST WRVGE D    + F
Sbjct: 74  ESSEVDE-GIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYF 133

Query: 135 VETG------DDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILI-EKGLRL 194
           V  G        D+   +F+I++     Y   +CP      G P+C   GI I E G+R 
Sbjct: 134 VVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRR 193

Query: 195 LALDGSAFPFEFVRA 199
           LAL    F   F +A
Sbjct: 194 LALSDKPFLVMFKKA 205

[Close]

BLAST of CmaCh12G010860.1 vs. NCBI nr
Match: gi|449442849|ref|XP_004139193.1| (PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus])

HSP 1 Score: 316.6 bits (810), Expect = 3.0e-83
Identity = 153/200 (76.50%), Postives = 169/200 (84.50%), Query Frame = 1


Query: 1   MMLCKSLVA-FSYLCLVMAASSIAQ--SPPVLDIEGRPLEINVEYFIKPAITDVAGNLTL 60
           M   KSL+A FS+LC  MA  S AQ   PPVLD EGRPL+ +VEYFIKPAITDVAGNLTL
Sbjct: 2   MRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 61

Query: 61  LTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAW 120
           +TRKGDQCPF+VGQVPL SQEIGFGVT TPYL  +D IREGRDLK VFQAF+ICIT T W
Sbjct: 62  ITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQW 121

Query: 121 RVGEADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILI 180
           +VGE DP +GRRFV+ G+D+TATGYFRID+ N GVYNIGWCPSDVP +GRPRCG AGILI
Sbjct: 122 KVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILI 181

Query: 181 EKGLRLLALDGSAFPFEFVR 198
           EKG+R LALDG AFPFEFVR
Sbjct: 182 EKGVRFLALDGPAFPFEFVR 201

[Close]

BLAST of CmaCh12G010860.1 vs. NCBI nr
Match: gi|659070428|ref|XP_008455022.1| (PREDICTED: miraculin-like isoform X2 [Cucumis melo])

HSP 1 Score: 308.1 bits (788), Expect = 1.1e-80
Identity = 149/200 (74.50%), Postives = 162/200 (81.00%), Query Frame = 1


Query: 1   MMLCKSLVAFSYLCLVMAASSIA---QSPPVLDIEGRPLEINVEYFIKPAITDVAGNLTL 60
           MML KSL  FS LC  MA  S A     PPVLD EGRPL+ + EYFIKPAITDVAGNLTL
Sbjct: 2   MMLHKSLAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTL 61

Query: 61  LTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAW 120
           +TRKGDQCPF+VGQVPL SQE GF V+  PY   ED IREGRDLK VFQ F+ICIT T W
Sbjct: 62  ITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQW 121

Query: 121 RVGEADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILI 180
           +VGEADP TGRRFV+ G D+TATGYFRID+ + GVYNIGWCPSDVPI+GRPRCG AGILI
Sbjct: 122 KVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILI 181

Query: 181 EKGLRLLALDGSAFPFEFVR 198
           EKG+R LALDG AFPFEFVR
Sbjct: 182 EKGVRFLALDGPAFPFEFVR 201

[Close]

BLAST of CmaCh12G010860.1 vs. NCBI nr
Match: gi|659070426|ref|XP_008455014.1| (PREDICTED: miraculin-like isoform X1 [Cucumis melo])

HSP 1 Score: 300.1 bits (767), Expect = 2.9e-78
Identity = 147/201 (73.13%), Postives = 161/201 (80.10%), Query Frame = 1


Query: 1   MMLCKSLVA-FSYLCLVMAASSIA---QSPPVLDIEGRPLEINVEYFIKPAITDVAGNLT 60
           M   KSL + FS LC  MA  S A     PPVLD EGRPL+ + EYFIKPAITDVAGNLT
Sbjct: 2   MRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLT 61

Query: 61  LLTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTA 120
           L+TRKGDQCPF+VGQVPL SQE GF V+  PY   ED IREGRDLK VFQ F+ICIT T 
Sbjct: 62  LITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQ 121

Query: 121 WRVGEADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGIL 180
           W+VGEADP TGRRFV+ G D+TATGYFRID+ + GVYNIGWCPSDVPI+GRPRCG AGIL
Sbjct: 122 WKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGIL 181

Query: 181 IEKGLRLLALDGSAFPFEFVR 198
           IEKG+R LALDG AFPFEFVR
Sbjct: 182 IEKGVRFLALDGPAFPFEFVR 202

[Close]

BLAST of CmaCh12G010860.1 vs. NCBI nr
Match: gi|449442847|ref|XP_004139192.1| (PREDICTED: miraculin-like [Cucumis sativus])

HSP 1 Score: 233.0 bits (593), Expect = 4.4e-58
Identity = 117/198 (59.09%), Postives = 135/198 (68.18%), Query Frame = 1


Query: 1   MMLCKSLVAFSYLCLVMAASSIAQSPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTR 60
           MML KSL  F Y CL MA +S AQ PPVLD  G+PL+  VEY+I PAITDV GNLTL +R
Sbjct: 2   MMLHKSLAIFGYACLFMAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSR 61

Query: 61  KGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVG 120
               CP  VGQ P+ S  IG  VTF P     D+I EG  L +VFQA S C+TST WRV 
Sbjct: 62  SNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVD 121

Query: 121 EADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKG 180
             +  TGRRFV  GD+D   G F I RDN G YNI WCP+   + GRPRCGRAGIL+E G
Sbjct: 122 ATESDTGRRFVGIGDEDGPAGIFGISRDN-GAYNIVWCPA---MMGRPRCGRAGILVENG 181

Query: 181 LRLLALDGSAFPFEFVRA 199
           +RL+ALDG AFPFEF++A
Sbjct: 182 VRLVALDGDAFPFEFIKA 195

[Close]

BLAST of CmaCh12G010860.1 vs. NCBI nr
Match: gi|242346705|gb|ACS92521.1| (Kunitz-type protease inhibitor KPI-F4 [Populus trichocarpa x Populus deltoides])

HSP 1 Score: 222.2 bits (565), Expect = 7.8e-55
Identity = 119/195 (61.03%), Postives = 134/195 (68.72%), Query Frame = 1


Query: 11  SYLCLVMAASSIAQ-----SPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQC 70
           S++ L MA  S+AQ     SPPVLD  G+PLE  VEY+I P ITDVAG LTL+ R G +C
Sbjct: 9   SFVWLTMAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRC 68

Query: 71  PFHVGQVPLRSQE-IGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGEADP 130
           PF+VGQ PL S E  G  V FTPY   E +IRE RDL V FQA +ICI STAWRVGE DP
Sbjct: 69  PFYVGQEPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDP 128

Query: 131 TTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSD-VPIRGRPRCGRAGILIEKGLRL 190
            TGRRF+ T  D +   YFRID +N GVYN  WCP++  P   RPRCG AGILIE   RL
Sbjct: 129 ETGRRFIVTAGDKS---YFRID-NNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKRL 188

Query: 191 LALDGSAFPFEFVRA 199
           LALDG AFPF F RA
Sbjct: 189 LALDGPAFPFVFTRA 199

The following BLAST results are available for this feature:

BLAST of CmaCh12G010860.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MIRA_SYNDU	5.8e-18	34.98	Miraculin OS=Synsepalum dulcificum PE=1 SV=3	[more]
IAAS_ORYSJ	9.6e-13	36.50	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 ...	[more]
IAAS_WHEAT	2.8e-12	33.33	Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1	[more]
ASP_THECC	5.0e-09	29.27	21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1	[more]
PIP21_PEA	1.4e-08	28.85	Kunitz-type trypsin inhibitor-like 1 protein OS=Pisum sativum GN=PIP20-1 PE=1 SV...	[more]

BLAST of CmaCh12G010860.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LGB4_CUCSA	2.1e-83	76.50	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021000 PE=4 SV=1	[more]
D1KFM4_9ROSI	5.4e-55	61.03	Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus nigra PE=...	[more]
G0Z2L7_POPNI	5.4e-55	60.51	Kunitz-type trypsin inhibitor C5 OS=Populus nigra PE=2 SV=1	[more]
D1KFM3_9ROSI	5.4e-55	61.03	Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides...	[more]
B9N8J2_POPTR	9.3e-55	61.03	Truncated Kunitz trypsin inhibitor family protein OS=Populus trichocarpa GN=POPT...	[more]

BLAST of CmaCh12G010860.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G73260.1	2.4e-17	31.28	kunitz trypsin inhibitor 1	[more]

BLAST of CmaCh12G010860.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449442849\|ref\|XP_004139193.1\|	3.0e-83	76.50	PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus]	[more]
gi\|659070428\|ref\|XP_008455022.1\|	1.1e-80	74.50	PREDICTED: miraculin-like isoform X2 [Cucumis melo]	[more]
gi\|659070426\|ref\|XP_008455014.1\|	2.9e-78	73.13	PREDICTED: miraculin-like isoform X1 [Cucumis melo]	[more]
gi\|449442847\|ref\|XP_004139192.1\|	4.4e-58	59.09	PREDICTED: miraculin-like [Cucumis sativus]	[more]
gi\|242346705\|gb\|ACS92521.1\|	7.8e-55	61.03	Kunitz-type protease inhibitor KPI-F4 [Populus trichocarpa x Populus deltoides]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR002160	Prot_inh_Kunz-lg
IPR011065	Kunitz_inhibitor_STI-like_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0004866	endopeptidase inhibitor activity

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004866	endopeptidase inhibitor activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh12G010860	CmaCh12G010860	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh12G010860.1	CmaCh12G010860.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh12G010860.1.exon.1	CmaCh12G010860.1.exon.1	exon
CmaCh12G010860.1.exon.2	CmaCh12G010860.1.exon.2	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh12G010860.1.five_prime_UTR.1	CmaCh12G010860.1.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh12G010860.1.CDS.1	CmaCh12G010860.1.CDS.1	CDS
CmaCh12G010860.1.CDS.2	CmaCh12G010860.1.CDS.2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh12G010860.1.three_prime_UTR.1	CmaCh12G010860.1.three_prime_UTR.1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 28..196 score: 1.8
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 27..198 score: 1.3
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 28..44 scor
IPR011065	Kunitz inhibitor ST1-like	unknown	SSF50386	STI-like	coord: 26..197 score: 6.35
None	No IPR available	GENE3D	G3DSA:2.80.10.50		coord: 25..198 score: 6.8
None	No IPR available	PANTHER	PTHR33107	FAMILY NOT NAMED	coord: 5..198 score: 3.8
None	No IPR available	PANTHER	PTHR33107:SF4	SUBFAMILY NOT NAMED	coord: 5..198 score: 3.8