Carg12880 (gene) Silver-seed gourd

NameCarg12880
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionRPM1-interacting protein 4-like
LocationCucurbita_argyrosperma_scaffold_043 : 899371 .. 900286 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGTCGCCTCCCTCTCTCTCTCTCAATCCGTCAGTGTTGTTTTCTCCCTTCGATTGATTTTGAAATTCCATTTTTTTTTTCCTCGGGAGCGTTTCTCTCTCCAGAATTCATGACGGTATGTTCTGGTTTGATTTCTGCGTCTTAAGAATATTATTACAATTTTCATTTTCAGCTTTGATTTTTTCTTTGATGTGCTTTCCGATCGGTTCGAGTTTTGCTTCCTATTGAGTTTTCGTTTGTTTGATTCGAATCGCCACTTTTGATTTGTTTATTCATTTGATTTTGTTAAAGATTGTTCAATTCTGCGTTTCTAGATTTTTTTTTTTCTTTCGTTTTCCTGAGATTGATGTTGATTGATTCTGGATTAGTTAAATTGGGCAGAAATCTGGATTTTTGTGCAAGATTAATTAATTTCTTGGAGGTCGTTTCTGAATGAATAATTCTGACAGCTGCATTCAAATATGTGACGTAGTTGGAGTAATTGATGTTGGAGGAAACTTTTTAATTGGTCTATTTTGTTTTGTTTGATTGAGTAATATCGATATTTTCAACATCTGTTGCTATAAACCAATGTTGTATGGTAATAAATGATGGTTGTTAGATTCGTAGTCCGATTGGTAAGGTAAGCCATCAGATTCACCGAGGGTACATCTTGTTTGTTCTTTCTTTTTGTAGGACAAAGGTCGGCCATTACCAAAGTTTGGTGAGTGGGATGTTAATGATCCGACATCAGCTGAGGGGTTCACCGTGATTTTTAACAAAGCTAGGGATGAGAAGAAAACAGGCGGCAAACCTGATTCACCTGGAAAAGTTGATGCACAGGGCAGGTCTGGAGCGGATCCTGCCAAGGCACCGCCTGTGAGATACATTCATAATCCTTTTTCTTTTGTGCTATTGAATGGTGGTTATAGCTAG

mRNA sequence

AAAGTCGCCTCCCTCTCTCTCTCTCAATCCGTCAGTGTTGTTTTCTCCCTTCGATTGATTTTGAAATTCCATTTTTTTTTTCCTCGGGAGCGTTTCTCTCTCCAGAATTCATGACGGACAAAGGTCGGCCATTACCAAAGTTTGGTGAGTGGGATGTTAATGATCCGACATCAGCTGAGGGGTTCACCGTGATTTTTAACAAAGCTAGGGATGAGAAGAAAACAGGCGGCAAACCTGATTCACCTGGAAAAGTTGATGCACAGGGCAGGTCTGGAGCGGATCCTGCCAAGGCACCGCCTGTGAGATACATTCATAATCCTTTTTCTTTTGTGCTATTGAATGGTGGTTATAGCTAG

Coding sequence (CDS)

ATGACGGACAAAGGTCGGCCATTACCAAAGTTTGGTGAGTGGGATGTTAATGATCCGACATCAGCTGAGGGGTTCACCGTGATTTTTAACAAAGCTAGGGATGAGAAGAAAACAGGCGGCAAACCTGATTCACCTGGAAAAGTTGATGCACAGGGCAGGTCTGGAGCGGATCCTGCCAAGGCACCGCCTGTGAGATACATTCATAATCCTTTTTCTTTTGTGCTATTGAATGGTGGTTATAGCTAG

Protein sequence

MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAKAPPVRYIHNPFSFVLLNGGYS
BLAST of Carg12880 vs. NCBI nr
Match: XP_008463014.1 (PREDICTED: RPM1-interacting protein 4-like isoform X1 [Cucumis melo])

HSP 1 Score: 145.2 bits (365), Expect = 9.6e-32
Identity = 68/78 (87.18%), Postives = 70/78 (89.74%), Query Frame = 0

Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDA GR+  DPAK
Sbjct: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAHGRTAPDPAK 60

Query: 61 APPVRYIHNPFSFVLLNG 79
          APPVRYIHN F    L+G
Sbjct: 61 APPVRYIHNLFPIWALDG 78

BLAST of Carg12880 vs. NCBI nr
Match: XP_022975420.1 (uncharacterized protein LOC111474733 isoform X1 [Cucurbita maxima])

HSP 1 Score: 139.0 bits (349), Expect = 6.9e-30
Identity = 67/79 (84.81%), Postives = 71/79 (89.87%), Query Frame = 0

Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK
Sbjct: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60

Query: 61 APPVRY---IHNPFSFVLL 77
          APP ++   I + F F+ L
Sbjct: 61 APPKKWLCCIQSLFDFIYL 79

BLAST of Carg12880 vs. NCBI nr
Match: XP_023535777.1 (uncharacterized protein LOC111797108 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 139.0 bits (349), Expect = 6.9e-30
Identity = 67/79 (84.81%), Postives = 71/79 (89.87%), Query Frame = 0

Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK
Sbjct: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60

Query: 61 APPVRY---IHNPFSFVLL 77
          APP ++   I + F F+ L
Sbjct: 61 APPKKWLCCIQSLFDFIYL 79

BLAST of Carg12880 vs. NCBI nr
Match: XP_022936748.1 (uncharacterized protein LOC111443250 [Cucurbita moschata] >XP_022975427.1 uncharacterized protein LOC111474733 isoform X2 [Cucurbita maxima] >XP_023535778.1 uncharacterized protein LOC111797108 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 136.7 bits (343), Expect = 3.4e-29
Identity = 63/67 (94.03%), Postives = 66/67 (98.51%), Query Frame = 0

Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK
Sbjct: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60

Query: 61 APPVRYI 68
          APP +++
Sbjct: 61 APPKKWL 67

BLAST of Carg12880 vs. NCBI nr
Match: XP_022139562.1 (RPM1-interacting protein 4 [Momordica charantia])

HSP 1 Score: 132.1 bits (331), Expect = 8.4e-28
Identity = 60/67 (89.55%), Postives = 65/67 (97.01%), Query Frame = 0

Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDA+GR GADP+K
Sbjct: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDARGRPGADPSK 60

Query: 61 APPVRYI 68
          APP +++
Sbjct: 61 APPKKWL 67

BLAST of Carg12880 vs. TAIR10
Match: AT5G55850.2 (RPM1-interacting protein 4 (RIN4) family protein)

HSP 1 Score: 100.9 bits (250), Expect = 3.8e-22
Identity = 50/66 (75.76%), Postives = 54/66 (81.82%), Query Frame = 0

Query: 3  DKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGR---SGADPA 62
          DKGRPLPKFGEWDVNDP SAEGFTVIFNKARDEKKTGGKP SPGK  ++G     G DP+
Sbjct: 19 DKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGGKPGSPGK-SSEGHVKSGGGDPS 78

Query: 63 KAPPVR 66
          K  PV+
Sbjct: 79 KPQPVK 83

BLAST of Carg12880 vs. TAIR10
Match: AT2G04410.1 (RPM1-interacting protein 4 (RIN4) family protein)

HSP 1 Score: 92.4 bits (228), Expect = 1.3e-19
Identity = 42/47 (89.36%), Postives = 44/47 (93.62%), Query Frame = 0

Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGK 48
          M DKGRPLPKFGEWDVNDP+SAEGFTVIFNKAR+EKK GGK DSPGK
Sbjct: 1  MADKGRPLPKFGEWDVNDPSSAEGFTVIFNKARNEKKGGGKSDSPGK 47

BLAST of Carg12880 vs. TAIR10
Match: AT5G63270.1 (RPM1-interacting protein 4 (RIN4) family protein)

HSP 1 Score: 68.9 bits (167), Expect = 1.6e-12
Identity = 33/46 (71.74%), Postives = 38/46 (82.61%), Query Frame = 0

Query: 3  DKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKT----GGKPDS 45
          +KG+PLPKFGEWDVN+P SAEGFTVIF+KA DEKKT    G  P+S
Sbjct: 4  NKGKPLPKFGEWDVNNPASAEGFTVIFSKASDEKKTKKASGAGPNS 49

BLAST of Carg12880 vs. TAIR10
Match: AT5G40645.1 (RPM1-interacting protein 4 (RIN4) family protein)

HSP 1 Score: 68.6 bits (166), Expect = 2.1e-12
Identity = 33/55 (60.00%), Postives = 38/55 (69.09%), Query Frame = 0

Query: 6  RPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 61
          RPLPKFGEWDVNDP SAEGFTVIF KARD+KKT     +  +     +S  +P K
Sbjct: 13 RPLPKFGEWDVNDPASAEGFTVIFAKARDDKKTNASGRAASQRRDNNKSQDEPTK 67

BLAST of Carg12880 vs. TAIR10
Match: AT3G48450.1 (RPM1-interacting protein 4 (RIN4) family protein)

HSP 1 Score: 65.9 bits (159), Expect = 1.3e-11
Identity = 28/36 (77.78%), Postives = 32/36 (88.89%), Query Frame = 0

Query: 3  DKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKT 39
          ++ RPLPKFGEWD  +P SAEGFTVIFNKARD+KKT
Sbjct: 5  NRARPLPKFGEWDATNPGSAEGFTVIFNKARDDKKT 40

BLAST of Carg12880 vs. Swiss-Prot
Match: sp|O22633|NOI4_ARATH (Protein NOI4 OS=Arabidopsis thaliana OX=3702 GN=NOI4 PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 1.8e-21
Identity = 50/70 (71.43%), Postives = 57/70 (81.43%), Query Frame = 0

Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGR---SGAD 60
          M+DKGRPLPKFGEWDVNDP SAEGFTVIFNKARDEKKTGGKP SPGK  ++G     G D
Sbjct: 1  MSDKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGGKPGSPGK-SSEGHVKSGGGD 60

Query: 61 PAKAPPVRYI 68
          P+K  P +++
Sbjct: 61 PSKPQPKKWL 69

BLAST of Carg12880 vs. Swiss-Prot
Match: sp|Q8GYN5|RIN4_ARATH (RPM1-interacting protein 4 OS=Arabidopsis thaliana OX=3702 GN=RIN4 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 6.2e-06
Identity = 20/37 (54.05%), Postives = 30/37 (81.08%), Query Frame = 0

Query: 3   DKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTG 40
           +K   +PKFG+WD N+P+SA+G+T IFNK R+E+ +G
Sbjct: 143 EKVTVVPKFGDWDENNPSSADGYTHIFNKVREERSSG 179

BLAST of Carg12880 vs. TrEMBL
Match: tr|A0A1S3CI83|A0A1S3CI83_CUCME (RPM1-interacting protein 4-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501257 PE=4 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 6.4e-32
Identity = 68/78 (87.18%), Postives = 70/78 (89.74%), Query Frame = 0

Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDA GR+  DPAK
Sbjct: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAHGRTAPDPAK 60

Query: 61 APPVRYIHNPFSFVLLNG 79
          APPVRYIHN F    L+G
Sbjct: 61 APPVRYIHNLFPIWALDG 78

BLAST of Carg12880 vs. TrEMBL
Match: tr|A0A1S3CI91|A0A1S3CI91_CUCME (RPM1-interacting protein 4-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103501257 PE=4 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 3.6e-27
Identity = 59/67 (88.06%), Postives = 63/67 (94.03%), Query Frame = 0

Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDA GR+  DPAK
Sbjct: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAHGRTAPDPAK 60

Query: 61 APPVRYI 68
          APP +++
Sbjct: 61 APPKKWL 67

BLAST of Carg12880 vs. TrEMBL
Match: tr|A0A0A0K6Y6|A0A0A0K6Y6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G127370 PE=4 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 4.0e-26
Identity = 58/63 (92.06%), Postives = 59/63 (93.65%), Query Frame = 0

Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDA GR+  DPAK
Sbjct: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAHGRNAPDPAK 60

Query: 61 APP 64
           PP
Sbjct: 61 TPP 63

BLAST of Carg12880 vs. TrEMBL
Match: tr|A0A2H5P5S1|A0A2H5P5S1_CITUN (Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_106570 PE=4 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 4.1e-23
Identity = 54/66 (81.82%), Postives = 57/66 (86.36%), Query Frame = 0

Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          M+DKGRPLPKFGEWDVNDP SAEGFTVIFNKARDEKKTGGKPDSPGKVD   R G DPAK
Sbjct: 1  MSDKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGGKPDSPGKVDPPIRQGVDPAK 60

Query: 61 APPVRY 67
            P ++
Sbjct: 61 PQPRKW 66

BLAST of Carg12880 vs. TrEMBL
Match: tr|V4UTF7|V4UTF7_9ROSI (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10010079mg PE=4 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 5.4e-23
Identity = 54/66 (81.82%), Postives = 57/66 (86.36%), Query Frame = 0

Query: 1  MTDKGRPLPKFGEWDVNDPTSAEGFTVIFNKARDEKKTGGKPDSPGKVDAQGRSGADPAK 60
          M+DKGRPLPKFGEWDVNDP SAEGFTVIFNKARDEKKTGGKPDSPGKVD   R G DPAK
Sbjct: 1  MSDKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGGKPDSPGKVDPPIRHGVDPAK 60

Query: 61 APPVRY 67
            P ++
Sbjct: 61 PQPRKW 66

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008463014.19.6e-3287.18PREDICTED: RPM1-interacting protein 4-like isoform X1 [Cucumis melo][more]
XP_022975420.16.9e-3084.81uncharacterized protein LOC111474733 isoform X1 [Cucurbita maxima][more]
XP_023535777.16.9e-3084.81uncharacterized protein LOC111797108 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022936748.13.4e-2994.03uncharacterized protein LOC111443250 [Cucurbita moschata] >XP_022975427.1 unchar... [more]
XP_022139562.18.4e-2889.55RPM1-interacting protein 4 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G55850.23.8e-2275.76RPM1-interacting protein 4 (RIN4) family protein[more]
AT2G04410.11.3e-1989.36RPM1-interacting protein 4 (RIN4) family protein[more]
AT5G63270.11.6e-1271.74RPM1-interacting protein 4 (RIN4) family protein[more]
AT5G40645.12.1e-1260.00RPM1-interacting protein 4 (RIN4) family protein[more]
AT3G48450.11.3e-1177.78RPM1-interacting protein 4 (RIN4) family protein[more]
Match NameE-valueIdentityDescription
sp|O22633|NOI4_ARATH1.8e-2171.43Protein NOI4 OS=Arabidopsis thaliana OX=3702 GN=NOI4 PE=1 SV=1[more]
sp|Q8GYN5|RIN4_ARATH6.2e-0654.05RPM1-interacting protein 4 OS=Arabidopsis thaliana OX=3702 GN=RIN4 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A1S3CI83|A0A1S3CI83_CUCME6.4e-3287.18RPM1-interacting protein 4-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC1035012... [more]
tr|A0A1S3CI91|A0A1S3CI91_CUCME3.6e-2788.06RPM1-interacting protein 4-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC1035012... [more]
tr|A0A0A0K6Y6|A0A0A0K6Y6_CUCSA4.0e-2692.06Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G127370 PE=4 SV=1[more]
tr|A0A2H5P5S1|A0A2H5P5S1_CITUN4.1e-2381.82Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_106570 PE=4 SV=1[more]
tr|V4UTF7|V4UTF7_9ROSI5.4e-2381.82Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10010079mg PE=4 ... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR008700TypeIII_avirulence_cleave
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12880-RACarg12880-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008700RIN4, pathogenic type III effector avirulence factor Avr cleavage sitePFAMPF05627AvrRpt-cleavagecoord: 3..38
e-value: 7.7E-22
score: 76.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..63
NoneNo IPR availablePANTHERPTHR33159FAMILY NOT NAMEDcoord: 2..66
NoneNo IPR availablePANTHERPTHR33159:SF25RPM1-INTERACTING PROTEIN 4 RIN4 FAMILY PROTEINcoord: 2..66