Carg12289 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg12289
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	MYB transcription factor
Location	Cucurbita_argyrosperma_scaffold_074 : 97221 .. 98295 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAGATCCCCTTGTTGTGATAAGGCCAATGTGAAGAAGGGCCCTTGGTCCCCTGAGGAGGATGCCAAGCTCAAGGCTTACATTCACAACCATGGCACTGGTGGCAATTGGATCTCCCTCCCTCAAAAAGCTGGTAAAAAAAAAAAAAAAATCACTTTTTTAGGGTTTTTTTTAGGGTTTTTTAGGGTTTTTTTTTAGGGTTTTTTTAGGGTTTTCAATCTCTAAGTGGGGTTTTTGAGTGTTTTTTTGTGTTTTTCTTGATGGGTTTTCTCTTTTTGTTTGTGGGTATTTGAAGGTTTGAAGAGATGTGGAAAGAGTTGTAGGTTGAGATGGCTTAACTATTTGAGGCCCAACATTAAGCATGGAGGCTTCACTGAGGAAGAAGATAGAATAATTTGTACCCTATTTTCCACTATTGGAAGCAGGTAATTTCTTTGCATGCATTTTTCGTTAAGCTTTTAAGTCGAGCGGTGATTTTTCGAATCTTGTTTAGGGTTTGGATTTAACTAAAGAAACTCGTAGGTGGTCGATAATTGCTACTCAGTTACCGGGAAGAACGGATAACGATATCAAAAATCATTGGAATAGCAAATTGAAGAAGAAACTTATAGGAATGGAGAAACACCCTCAAATCATCCCTCCTACTACAATGCCATATTCTTCTCATCATAATGATCATCAAGTTTCATCATTTCCAGCTCAACCACTCAGTTCAATCCCATCAAATTGGTCAAACAACAACGACAACGACAACGACAACATCGACAACATTAGCCCTTGTCTTGTCCCGACACAAGAATCCAACATGAACAGCTTGTTGAGCTCCTTGATGTTTCAGCATAATCAAACCCTAGATGGAAGAAGCTGCACTCAAATCAACAACACTTCAAGAATCATGATGATGGGGTTTCAGAATCAAACCCATAATTACAATTATTATTATGATGCTATGAATGATGAAGAACATAACAACATATGCTTCAATGGAGGAGGAGGAGGAGGAGATGATGATCATGTTCATAGCACCACCAGCTTGTTCCAATGCATGGAGGGAAATTTCATGAAGTTCTAG

mRNA sequence

ATGGGAAGATCCCCTTGTTGTGATAAGGCCAATGTGAAGAAGGGCCCTTGGTCCCCTGAGGAGGATGCCAAGCTCAAGGCTTACATTCACAACCATGGCACTGGTGGCAATTGGATCTCCCTCCCTCAAAAAGCTGGTTTGAAGAGATGTGGAAAGAGTTGTAGGTTGAGATGGCTTAACTATTTGAGGCCCAACATTAAGCATGGAGGCTTCACTGAGGAAGAAGATAGAATAATTTGTACCCTATTTTCCACTATTGGAAGCAGGTGGTCGATAATTGCTACTCAGTTACCGGGAAGAACGGATAACGATATCAAAAATCATTGGAATAGCAAATTGAAGAAGAAACTTATAGGAATGGAGAAACACCCTCAAATCATCCCTCCTACTACAATGCCATATTCTTCTCATCATAATGATCATCAAGTTTCATCATTTCCAGCTCAACCACTCAGTTCAATCCCATCAAATTGGTCAAACAACAACGACAACGACAACGACAACATCGACAACATTAGCCCTTGTCTTGTCCCGACACAAGAATCCAACATGAACAGCTTGTTGAGCTCCTTGATGTTTCAGCATAATCAAACCCTAGATGGAAGAAGCTGCACTCAAATCAACAACACTTCAAGAATCATGATGATGGGGTTTCAGAATCAAACCCATAATTACAATTATTATTATGATGCTATGAATGATGAAGAACATAACAACATATGCTTCAATGGAGGAGGAGGAGGAGGAGATGATGATCATGTTCATAGCACCACCAGCTTGTTCCAATGCATGGAGGGAAATTTCATGAAGTTCTAG

Coding sequence (CDS)

ATGGGAAGATCCCCTTGTTGTGATAAGGCCAATGTGAAGAAGGGCCCTTGGTCCCCTGAGGAGGATGCCAAGCTCAAGGCTTACATTCACAACCATGGCACTGGTGGCAATTGGATCTCCCTCCCTCAAAAAGCTGGTTTGAAGAGATGTGGAAAGAGTTGTAGGTTGAGATGGCTTAACTATTTGAGGCCCAACATTAAGCATGGAGGCTTCACTGAGGAAGAAGATAGAATAATTTGTACCCTATTTTCCACTATTGGAAGCAGGTGGTCGATAATTGCTACTCAGTTACCGGGAAGAACGGATAACGATATCAAAAATCATTGGAATAGCAAATTGAAGAAGAAACTTATAGGAATGGAGAAACACCCTCAAATCATCCCTCCTACTACAATGCCATATTCTTCTCATCATAATGATCATCAAGTTTCATCATTTCCAGCTCAACCACTCAGTTCAATCCCATCAAATTGGTCAAACAACAACGACAACGACAACGACAACATCGACAACATTAGCCCTTGTCTTGTCCCGACACAAGAATCCAACATGAACAGCTTGTTGAGCTCCTTGATGTTTCAGCATAATCAAACCCTAGATGGAAGAAGCTGCACTCAAATCAACAACACTTCAAGAATCATGATGATGGGGTTTCAGAATCAAACCCATAATTACAATTATTATTATGATGCTATGAATGATGAAGAACATAACAACATATGCTTCAATGGAGGAGGAGGAGGAGGAGATGATGATCATGTTCATAGCACCACCAGCTTGTTCCAATGCATGGAGGGAAATTTCATGAAGTTCTAG

Protein sequence

MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLNYLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGMEKHPQIIPPTTMPYSSHHNDHQVSSFPAQPLSSIPSNWSNNNDNDNDNIDNISPCLVPTQESNMNSLLSSLMFQHNQTLDGRSCTQINNTSRIMMMGFQNQTHNYNYYYDAMNDEEHNNICFNGGGGGGDDDHVHSTTSLFQCMEGNFMKF

Homology

[Close]

BLAST of Carg12289 vs. NCBI nr
Match: XP_022948843.1 (transcription factor MYB36-like [Cucurbita moschata])

HSP 1 Score: 481.1 bits (1237), Expect = 2.5e-132
Identity = 271/271 (100.00%), Postives = 271/271 (100.00%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN
Sbjct: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120
           YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM
Sbjct: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120

Query: 121 EKHPQIIPPTTMPYSSHHNDHQVSSFPAQPLSSIPSNWSXXXXXXXXXXXXISPCLVPTQ 180
           EKHPQIIPPTTMPYSSHHNDHQVSSFPAQPLSSIPSNWSXXXXXXXXXXXXISPCLVPTQ
Sbjct: 121 EKHPQIIPPTTMPYSSHHNDHQVSSFPAQPLSSIPSNWSXXXXXXXXXXXXISPCLVPTQ 180

Query: 181 ESNMNSLLSSLMFQHNQTLDGRSCTQINNTSRIMMMGFQNQTHNYNXXXXXXXXXXXXXX 240
           ESNMNSLLSSLMFQHNQTLDGRSCTQINNTSRIMMMGFQNQTHNYNXXXXXXXXXXXXXX
Sbjct: 181 ESNMNSLLSSLMFQHNQTLDGRSCTQINNTSRIMMMGFQNQTHNYNXXXXXXXXXXXXXX 240

Query: 241 XXXXXXXXXXXXXXHSTTSLFQCMEGNFMKF 272
           XXXXXXXXXXXXXXHSTTSLFQCMEGNFMKF
Sbjct: 241 XXXXXXXXXXXXXXHSTTSLFQCMEGNFMKF 271

[Close]

BLAST of Carg12289 vs. NCBI nr
Match: XP_023524586.1 (transcription factor MYB36-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 463.8 bits (1192), Expect = 4.1e-127
Identity = 264/272 (97.06%), Postives = 266/272 (97.79%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN
Sbjct: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120
           YLRPNIKHGGFTEEEDRIICTL+STIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM
Sbjct: 61  YLRPNIKHGGFTEEEDRIICTLYSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120

Query: 121 EKHPQIIPPTTMPYSSHHNDHQVSSFPAQPLSSIPSNWSXXXXXXXXXXXXISPCLVPTQ 180
           EK+PQIIPPTTMPYSSHHNDHQVSSFPAQPLSSIPSNWS   XXXXXXXXXISPCLVPTQ
Sbjct: 121 EKNPQIIPPTTMPYSSHHNDHQVSSFPAQPLSSIPSNWS-NDXXXXXXXXXISPCLVPTQ 180

Query: 181 ESNMNSLLSSLMFQHNQTLDGRSCTQINNTSRIMMMGFQNQTHNYN-XXXXXXXXXXXXX 240
           ESNMNSLLSSLMFQHNQTLD RSCTQINNTSRIMMMGFQNQTHNYN XXXXXXXXXXXXX
Sbjct: 181 ESNMNSLLSSLMFQHNQTLDARSCTQINNTSRIMMMGFQNQTHNYNXXXXXXXXXXXXXX 240

Query: 241 XXXXXXXXXXXXXXXHSTTSLFQCMEGNFMKF 272
           XXXXXXXXXXXXXXXHSTTSLFQCMEG FMKF
Sbjct: 241 XXXXXXXXXXXXXXXHSTTSLFQCMEGKFMKF 271

[Close]

BLAST of Carg12289 vs. NCBI nr
Match: XP_022998892.1 (transcription factor MYB36-like [Cucurbita maxima])

HSP 1 Score: 458.4 bits (1178), Expect = 1.7e-125
Identity = 255/274 (93.07%), Postives = 256/274 (93.43%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN
Sbjct: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120
           YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM
Sbjct: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120

Query: 121 EKHPQIIPPTTMPYSSHHNDHQVSSFPAQPLSSIPSNWSXXXXXXXXXXXXISPCLVPTQ 180
           EKHPQII PTTMPYSSHHNDHQVSSFPAQPLSSIPSNWS            ISPCLVPTQ
Sbjct: 121 EKHPQII-PTTMPYSSHHNDHQVSSFPAQPLSSIPSNWS-------NDNDNISPCLVPTQ 180

Query: 181 ESNMNSLLSSLMFQHNQTLDGRSCTQINNTSRIMMMGFQNQTHNYN---XXXXXXXXXXX 240
           ESNMNSLLSSLM+QHNQTLDGRSCTQINNTSRIMMMGFQNQ HNYN   XXXXXXXXXXX
Sbjct: 181 ESNMNSLLSSLMYQHNQTLDGRSCTQINNTSRIMMMGFQNQPHNYNXXXXXXXXXXXXXX 240

Query: 241 XXXXXXXXXXXXXXXXXHSTTSLFQCMEGNFMKF 272
           XXXXXXXXXXXXXXXXXHSTTSLFQCMEG FMKF
Sbjct: 241 XXXXXXXXXXXXXXXXXHSTTSLFQCMEGKFMKF 266

[Close]

BLAST of Carg12289 vs. NCBI nr
Match: XP_022964958.1 (transcription factor MYB87-like [Cucurbita moschata])

HSP 1 Score: 290.4 bits (742), Expect = 6.2e-75
Identity = 157/249 (63.05%), Postives = 176/249 (70.68%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGRSPCCDKA VKKGPWSPEEDAKLKAYI NHGT GNWISLPQKAGL RCGKSCRLRWLN
Sbjct: 1   MGRSPCCDKAIVKKGPWSPEEDAKLKAYIINHGTAGNWISLPQKAGLNRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120
           YLRPNIKHGGFT+EEDRIICTLFSTIGSRWSIIA+QLPGRTDNDIKNHWNSKLK+KLI M
Sbjct: 61  YLRPNIKHGGFTDEEDRIICTLFSTIGSRWSIIASQLPGRTDNDIKNHWNSKLKRKLIAM 120

Query: 121 EKHPQIIPPTTMPYSSHHNDHQVSSFPAQPL-------------SSIPSNWSXXXXXXXX 180
           EK  Q+    TMP  S HN HQ+SSFP+Q L             SSIPSNWS        
Sbjct: 121 EKKAQM---GTMP--SFHNHHQISSFPSQQLYQDSNFSSFETLISSIPSNWSN------- 180

Query: 181 XXXXISPCLVPTQESNMNSLL-SSLMFQHNQTL---------DGRSCT-QINNTSRIMMM 226
                    + T+E+N NSL+ +SLM+ +N  +         +G +C    N  +   +M
Sbjct: 181 ---------IQTKETNSNSLMNNSLMYNNNPMVKQSSLGFGSEGSTCDGSYNQITPSGIM 227

[Close]

BLAST of Carg12289 vs. NCBI nr
Match: XP_023520293.1 (transcription factor MYB87-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 288.9 bits (738), Expect = 1.8e-74
Identity = 156/250 (62.40%), Postives = 175/250 (70.00%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGRSPCCDKA VKKGPWSPEEDAKLKAYI NHGT GNWISLPQKAGL RCGKSCRLRWLN
Sbjct: 1   MGRSPCCDKAIVKKGPWSPEEDAKLKAYIINHGTAGNWISLPQKAGLNRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120
           YLRPNIKHGGFT+EEDRIICTLFSTIGSRWSIIA+QLPGRTDNDIKNHWNSKLK+KLI M
Sbjct: 61  YLRPNIKHGGFTDEEDRIICTLFSTIGSRWSIIASQLPGRTDNDIKNHWNSKLKRKLIAM 120

Query: 121 EKHPQIIPPTTMPYSSHHNDHQVSSFPAQPL-------------SSIPSNWSXXXXXXXX 180
           EK  Q+    TMP  S HN HQ+SSFP+Q L             SSIPSNWS        
Sbjct: 121 EKKAQM---GTMP--SFHNHHQISSFPSQQLYQDSNFSSFETLISSIPSNWSN------- 180

Query: 181 XXXXISPCLVPTQESNMNSLLS-SLMFQHNQTL----------DGRSCT-QINNTSRIMM 226
                    + T+E+N NSL++  LM+ +N  +          +G +C    N  +   +
Sbjct: 181 ---------IQTKETNSNSLMNXXLMYNNNNPMVKQSSLGFGSEGSTCDGSYNQITPSGI 228

[Close]

BLAST of Carg12289 vs. TAIR10
Match: AT3G49690.1 (myb domain protein 84)

HSP 1 Score: 235.7 bits (600), Expect = 3.3e-62
Identity = 102/122 (83.61%), Postives = 114/122 (93.44%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGR+PCCDKANVKKGPWSPEEDAKLK+YI N GTGGNWI+LPQK GLKRCGKSCRLRWLN
Sbjct: 1   MGRAPCCDKANVKKGPWSPEEDAKLKSYIENSGTGGNWIALPQKIGLKRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120
           YLRPNIKHGGF+EEE+ IIC+L+ TIGSRWSIIA QLPGRTDNDIKN+WN++LKKKLI  
Sbjct: 61  YLRPNIKHGGFSEEEENIICSLYLTIGSRWSIIAAQLPGRTDNDIKNYWNTRLKKKLINK 120

Query: 121 EK 123
           ++
Sbjct: 121 QR 122

[Close]

BLAST of Carg12289 vs. TAIR10
Match: AT5G65790.1 (myb domain protein 68)

HSP 1 Score: 233.8 bits (595), Expect = 1.3e-61
Identity = 101/122 (82.79%), Postives = 112/122 (91.80%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGR+PCCDKANVKKGPWSPEEDAKLK YI N GTGGNWI+LPQK GL+RCGKSCRLRWLN
Sbjct: 1   MGRAPCCDKANVKKGPWSPEEDAKLKDYIENSGTGGNWIALPQKIGLRRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120
           YLRPNIKHGGF+EEED IIC L+ TIGSRWSIIA QLPGRTDNDIKN+WN++LKKKL+  
Sbjct: 61  YLRPNIKHGGFSEEEDNIICNLYVTIGSRWSIIAAQLPGRTDNDIKNYWNTRLKKKLLNK 120

Query: 121 EK 123
           ++
Sbjct: 121 QR 122

[Close]

BLAST of Carg12289 vs. TAIR10
Match: AT5G57620.1 (myb domain protein 36)

HSP 1 Score: 233.4 bits (594), Expect = 1.6e-61
Identity = 102/122 (83.61%), Postives = 113/122 (92.62%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGR+PCCDKANVKKGPWSPEED KLK YI  +GTGGNWI+LPQK GLKRCGKSCRLRWLN
Sbjct: 1   MGRAPCCDKANVKKGPWSPEEDVKLKDYIDKYGTGGNWIALPQKIGLKRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120
           YLRPNIKHGGF+EEEDRII +L+ +IGSRWSIIA QLPGRTDNDIKN+WN+KLKKKL+G 
Sbjct: 61  YLRPNIKHGGFSEEEDRIILSLYISIGSRWSIIAAQLPGRTDNDIKNYWNTKLKKKLLGR 120

Query: 121 EK 123
           +K
Sbjct: 121 QK 122

[Close]

BLAST of Carg12289 vs. TAIR10
Match: AT4G37780.1 (myb domain protein 87)

HSP 1 Score: 222.6 bits (566), Expect = 2.9e-58
Identity = 95/121 (78.51%), Postives = 109/121 (90.08%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGR+PCCDK  VKKGPWS EEDA LK+YI  HGTG NWISLPQ+ G+KRCGKSCRLRWLN
Sbjct: 1   MGRAPCCDKMAVKKGPWSTEEDAVLKSYIEKHGTGNNWISLPQRIGIKRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120
           YLRPN+KHGGFT+EED IIC+L+ TIGSRWSIIA+QLPGRTDNDIKN+WN++LKKKL+  
Sbjct: 61  YLRPNLKHGGFTDEEDYIICSLYITIGSRWSIIASQLPGRTDNDIKNYWNTRLKKKLLSK 120

Query: 121 E 122
           +
Sbjct: 121 Q 121

[Close]

BLAST of Carg12289 vs. TAIR10
Match: AT2G36890.1 (Duplicated homeodomain-like superfamily protein)

HSP 1 Score: 213.8 bits (543), Expect = 1.3e-55
Identity = 92/114 (80.70%), Postives = 103/114 (90.35%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGR+PCCDKANVK+GPWSPEEDAKLK YI   GTGGNWI+LP KAGL+RCGKSCRLRWLN
Sbjct: 1   MGRAPCCDKANVKRGPWSPEEDAKLKDYIEKQGTGGNWIALPHKAGLRRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLK 115
           YLRPNI+HG FTEEED II +LF++IGSRWS+IA  L GRTDNDIKN+WN+KLK
Sbjct: 61  YLRPNIRHGDFTEEEDNIIYSLFASIGSRWSVIAAHLQGRTDNDIKNYWNTKLK 114

[Close]

BLAST of Carg12289 vs. Swiss-Prot
Match: sp|Q9M2Y9|RAX3_ARATH (Transcription factor RAX3 OS=Arabidopsis thaliana OX=3702 GN=RAX3 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 5.9e-61
Identity = 102/122 (83.61%), Postives = 114/122 (93.44%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGR+PCCDKANVKKGPWSPEEDAKLK+YI N GTGGNWI+LPQK GLKRCGKSCRLRWLN
Sbjct: 1   MGRAPCCDKANVKKGPWSPEEDAKLKSYIENSGTGGNWIALPQKIGLKRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120
           YLRPNIKHGGF+EEE+ IIC+L+ TIGSRWSIIA QLPGRTDNDIKN+WN++LKKKLI  
Sbjct: 61  YLRPNIKHGGFSEEEENIICSLYLTIGSRWSIIAAQLPGRTDNDIKNYWNTRLKKKLINK 120

Query: 121 EK 123
           ++
Sbjct: 121 QR 122

[Close]

BLAST of Carg12289 vs. Swiss-Prot
Match: sp|Q9FKL2|MYB36_ARATH (Transcription factor MYB36 OS=Arabidopsis thaliana OX=3702 GN=MYB36 PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 2.9e-60
Identity = 102/122 (83.61%), Postives = 113/122 (92.62%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGR+PCCDKANVKKGPWSPEED KLK YI  +GTGGNWI+LPQK GLKRCGKSCRLRWLN
Sbjct: 1   MGRAPCCDKANVKKGPWSPEEDVKLKDYIDKYGTGGNWIALPQKIGLKRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120
           YLRPNIKHGGF+EEEDRII +L+ +IGSRWSIIA QLPGRTDNDIKN+WN+KLKKKL+G 
Sbjct: 61  YLRPNIKHGGFSEEEDRIILSLYISIGSRWSIIAAQLPGRTDNDIKNYWNTKLKKKLLGR 120

Query: 121 EK 123
           +K
Sbjct: 121 QK 122

[Close]

BLAST of Carg12289 vs. Swiss-Prot
Match: sp|F4JSU0|MYB87_ARATH (Transcription factor MYB87 OS=Arabidopsis thaliana OX=3702 GN=MYB87 PE=2 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 5.2e-57
Identity = 95/121 (78.51%), Postives = 109/121 (90.08%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGR+PCCDK  VKKGPWS EEDA LK+YI  HGTG NWISLPQ+ G+KRCGKSCRLRWLN
Sbjct: 1   MGRAPCCDKMAVKKGPWSTEEDAVLKSYIEKHGTGNNWISLPQRIGIKRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120
           YLRPN+KHGGFT+EED IIC+L+ TIGSRWSIIA+QLPGRTDNDIKN+WN++LKKKL+  
Sbjct: 61  YLRPNLKHGGFTDEEDYIICSLYITIGSRWSIIASQLPGRTDNDIKNYWNTRLKKKLLSK 120

Query: 121 E 122
           +
Sbjct: 121 Q 121

[Close]

BLAST of Carg12289 vs. Swiss-Prot
Match: sp|Q9SJL7|RAX2_ARATH (Transcription factor RAX2 OS=Arabidopsis thaliana OX=3702 GN=RAX2 PE=1 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 2.4e-54
Identity = 92/114 (80.70%), Postives = 103/114 (90.35%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGR+PCCDKANVK+GPWSPEEDAKLK YI   GTGGNWI+LP KAGL+RCGKSCRLRWLN
Sbjct: 1   MGRAPCCDKANVKRGPWSPEEDAKLKDYIEKQGTGGNWIALPHKAGLRRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLK 115
           YLRPNI+HG FTEEED II +LF++IGSRWS+IA  L GRTDNDIKN+WN+KLK
Sbjct: 61  YLRPNIRHGDFTEEEDNIIYSLFASIGSRWSVIAAHLQGRTDNDIKNYWNTKLK 114

[Close]

BLAST of Carg12289 vs. Swiss-Prot
Match: sp|Q9FG68|RAX1_ARATH (Transcription factor RAX1 OS=Arabidopsis thaliana OX=3702 GN=RAX1 PE=1 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 7.0e-54
Identity = 91/116 (78.45%), Postives = 103/116 (88.79%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGR+PCCDK  VK+GPWSPEED+KL+ YI  +G GGNWIS P KAGL+RCGKSCRLRWLN
Sbjct: 1   MGRAPCCDKTKVKRGPWSPEEDSKLRDYIEKYGNGGNWISFPLKAGLRRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKK 117
           YLRPNIKHG F+EEEDRII +LF+ IGSRWSIIA  LPGRTDNDIKN+WN+KL+KK
Sbjct: 61  YLRPNIKHGDFSEEEDRIIFSLFAAIGSRWSIIAAHLPGRTDNDIKNYWNTKLRKK 116

[Close]

BLAST of Carg12289 vs. TrEMBL
Match: tr|A0A0A0LUZ6|A0A0A0LUZ6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G109320 PE=4 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 3.3e-72
Identity = 160/277 (57.76%), Postives = 178/277 (64.26%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGRSPCCDK+NVKKGPWSPEEDAKLK +I  +GTGGNWISLPQKAGLKRCGKSCRLRWLN
Sbjct: 1   MGRSPCCDKSNVKKGPWSPEEDAKLKDFIDKNGTGGNWISLPQKAGLKRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120
           YLRPNIKHGGFT+EEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLK+KLI M
Sbjct: 61  YLRPNIKHGGFTDEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKRKLIEM 120

Query: 121 EKHPQIIPPTTMP-------------YSS-----------------HHNDHQVSSFPA-- 180
           EK  QI    T+P             +SS                 +++++ +SS P   
Sbjct: 121 EKKAQITTSRTIPSXXXXXXXXXXXXFSSQPLSSLINKHDDYFNDINYSNYNLSSPPTTI 180

Query: 181 ----QPLSSIPSNWSXXXXXXXXXXXXISPCLVPTQESNMNSLLSSLMFQH--------- 220
                 +SSIPSNWS  XX         SPCLV TQE+      +SLM+ H         
Sbjct: 181 KSFESLISSIPSNWSNNXXNISP-----SPCLVQTQETTNLLSSNSLMYNHHNPLVQMKE 240

[Close]

BLAST of Carg12289 vs. TrEMBL
Match: tr|A0A2N9EYF9|A0A2N9EYF9_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS7662 PE=4 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.6e-63
Identity = 115/153 (75.16%), Postives = 129/153 (84.31%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGR+PCCDKANVKKGPWSPEED+KLK YI  +GTGGNWI+LPQKAGLKRCGKSCRLRWLN
Sbjct: 1   MGRAPCCDKANVKKGPWSPEEDSKLKEYIEKYGTGGNWIALPQKAGLKRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120
           YLRPNIKHG F++EEDRIICTLF++IGSRWSIIA QLPGRTDNDIKN+WN+KLKKKL+GM
Sbjct: 61  YLRPNIKHGEFSDEEDRIICTLFASIGSRWSIIAAQLPGRTDNDIKNYWNTKLKKKLMGM 120

Query: 121 EKHPQIIPPTTMPYSSHHNDHQVSSFPAQPLSS 154
                 +PP+        N HQ   F +QPLSS
Sbjct: 121 ------LPPSQRKPPPFPNSHQTPPFQSQPLSS 147

[Close]

BLAST of Carg12289 vs. TrEMBL
Match: tr|A0A2P4M0T2|A0A2P4M0T2_QUESU (Transcription factor myb36 OS=Quercus suber OX=58331 GN=CFP56_33206 PE=4 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 5.2e-62
Identity = 130/226 (57.52%), Postives = 153/226 (67.70%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGR+PCCDKANVKKGPWSPEED+KLK YI  +GTGGNWI+LPQKAGLKRCGKSCRLRWLN
Sbjct: 1   MGRAPCCDKANVKKGPWSPEEDSKLKEYIEKYGTGGNWIALPQKAGLKRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120
           YLRPNIKHG F++EEDRIICTLF++IGSRWSIIA QLPGRTDNDIKN+WN+KLKKKL+GM
Sbjct: 61  YLRPNIKHGEFSDEEDRIICTLFASIGSRWSIIAAQLPGRTDNDIKNYWNTKLKKKLMGM 120

Query: 121 EKHPQIIPPTTMPYSSHHNDHQVSSFPAQPLSSIPSNWSXXXXXXXXXXXXISPCLVPTQ 180
               Q  PP   P+ S    HQ   F +QPLS    + S            +    VP  
Sbjct: 121 LPPSQRKPP---PFPS---PHQGPPFQSQPLSYKECSLSYYSPTTTKSFSGLESMSVPLN 180

Query: 181 ESNMNSLLS----SLMFQHNQTLDGRSCTQINNTSRIMMMGFQNQT 223
            SN  + L+    S +FQ  ++  G S         ++M G +  T
Sbjct: 181 FSNTTTSLATNNPSSLFQTQESWLG-SMQYYPVKENLLMFGSEGST 219

[Close]

BLAST of Carg12289 vs. TrEMBL
Match: tr|A0A061GYH8|A0A061GYH8_THECC (Myb domain protein 36, putative OS=Theobroma cacao OX=3641 GN=TCM_042012 PE=4 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 6.8e-62
Identity = 116/154 (75.32%), Postives = 130/154 (84.42%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGR+PCCDKANVKKGPWSPEED+KLK YI  +GTGGNWI+LPQKAGLKRCGKSCRLRWLN
Sbjct: 1   MGRAPCCDKANVKKGPWSPEEDSKLKEYIEKYGTGGNWIALPQKAGLKRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120
           YLRPNIKHG FT+EEDRIIC+LF++IGSRWSIIA QLPGRTDNDIKN+WN+KLKKKL+ M
Sbjct: 61  YLRPNIKHGEFTDEEDRIICSLFASIGSRWSIIAAQLPGRTDNDIKNYWNTKLKKKLMAM 120

Query: 121 EKHPQIIPPTTMPYSSHHNDHQVSSFPAQPLSSI 155
               Q  PP   P+ S H+   VSS   Q LSS+
Sbjct: 121 TAQSQRKPP---PFPSSHHTPPVSS---QSLSSL 148

[Close]

BLAST of Carg12289 vs. TrEMBL
Match: tr|A0A1U8MJA5|A0A1U8MJA5_GOSHI (transcription factor RAX3-like OS=Gossypium hirsutum OX=3635 GN=LOC107938316 PE=4 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 8.9e-62
Identity = 111/146 (76.03%), Postives = 125/146 (85.62%), Query Frame = 0


Query: 1   MGRSPCCDKANVKKGPWSPEEDAKLKAYIHNHGTGGNWISLPQKAGLKRCGKSCRLRWLN 60
           MGR+PCCDKANVKKGPWSPEEDAKLKAYI N+GTGGNWISLPQK GLKRCGKSCRLRWLN
Sbjct: 1   MGRAPCCDKANVKKGPWSPEEDAKLKAYIENYGTGGNWISLPQKIGLKRCGKSCRLRWLN 60

Query: 61  YLRPNIKHGGFTEEEDRIICTLFSTIGSRWSIIATQLPGRTDNDIKNHWNSKLKKKLIGM 120
           YLRPNIKHGGF+EEED IIC+L+ +IGSRWSIIA QLPGRTDNDIKN+WN++LKKKL+G 
Sbjct: 61  YLRPNIKHGGFSEEEDEIICSLYVSIGSRWSIIAAQLPGRTDNDIKNYWNTRLKKKLLGR 120

Query: 121 EKHPQ-IIPPTTMPYSSHHNDHQVSS 146
              P+   P   +PYSS     Q ++
Sbjct: 121 YPRPEPPFPVVPVPYSSQEQSIQFTN 146

The following BLAST results are available for this feature:

BLAST of Carg12289 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022948843.1	2.5e-132	100.00	transcription factor MYB36-like [Cucurbita moschata]	[more]
XP_023524586.1	4.1e-127	97.06	transcription factor MYB36-like [Cucurbita pepo subsp. pepo]	[more]
XP_022998892.1	1.7e-125	93.07	transcription factor MYB36-like [Cucurbita maxima]	[more]
XP_022964958.1	6.2e-75	63.05	transcription factor MYB87-like [Cucurbita moschata]	[more]
XP_023520293.1	1.8e-74	62.40	transcription factor MYB87-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg12289 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G49690.1	3.3e-62	83.61	myb domain protein 84	[more]
AT5G65790.1	1.3e-61	82.79	myb domain protein 68	[more]
AT5G57620.1	1.6e-61	83.61	myb domain protein 36	[more]
AT4G37780.1	2.9e-58	78.51	myb domain protein 87	[more]
AT2G36890.1	1.3e-55	80.70	Duplicated homeodomain-like superfamily protein	[more]

BLAST of Carg12289 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9M2Y9\|RAX3_ARATH	5.9e-61	83.61	Transcription factor RAX3 OS=Arabidopsis thaliana OX=3702 GN=RAX3 PE=2 SV=1	[more]
sp\|Q9FKL2\|MYB36_ARATH	2.9e-60	83.61	Transcription factor MYB36 OS=Arabidopsis thaliana OX=3702 GN=MYB36 PE=1 SV=1	[more]
sp\|F4JSU0\|MYB87_ARATH	5.2e-57	78.51	Transcription factor MYB87 OS=Arabidopsis thaliana OX=3702 GN=MYB87 PE=2 SV=1	[more]
sp\|Q9SJL7\|RAX2_ARATH	2.4e-54	80.70	Transcription factor RAX2 OS=Arabidopsis thaliana OX=3702 GN=RAX2 PE=1 SV=1	[more]
sp\|Q9FG68\|RAX1_ARATH	7.0e-54	78.45	Transcription factor RAX1 OS=Arabidopsis thaliana OX=3702 GN=RAX1 PE=1 SV=1	[more]

BLAST of Carg12289 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LUZ6\|A0A0A0LUZ6_CUCSA	3.3e-72	57.76	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G109320 PE=4 SV=1	[more]
tr\|A0A2N9EYF9\|A0A2N9EYF9_FAGSY	1.6e-63	75.16	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS7662 PE=4 SV=1	[more]
tr\|A0A2P4M0T2\|A0A2P4M0T2_QUESU	5.2e-62	57.52	Transcription factor myb36 OS=Quercus suber OX=58331 GN=CFP56_33206 PE=4 SV=1	[more]
tr\|A0A061GYH8\|A0A061GYH8_THECC	6.8e-62	75.32	Myb domain protein 36, putative OS=Theobroma cacao OX=3641 GN=TCM_042012 PE=4 SV...	[more]
tr\|A0A1U8MJA5\|A0A1U8MJA5_GOSHI	8.9e-62	76.03	transcription factor RAX3-like OS=Gossypium hirsutum OX=3635 GN=LOC107938316 PE=...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding

Vocabulary: **INTERPRO**
Term	Definition
IPR009057	Homeobox-like_sf
IPR017930	Myb_dom
IPR001005	SANT/Myb

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003677	DNA binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12289-RA	Carg12289-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001005	SANT/Myb domain	SMART	SM00717	sant	coord: 67..115 e-value: 8.0E-12 score: 55.3 coord: 13..64 e-value: 1.5E-11 score: 54.3
IPR001005	SANT/Myb domain	PFAM	PF00249	Myb_DNA-binding	coord: 69..111 e-value: 1.1E-11 score: 44.5 coord: 14..62 e-value: 2.5E-15 score: 56.2
IPR001005	SANT/Myb domain	CDD	cd00167	SANT	coord: 71..113 e-value: 1.36975E-8 score: 48.7258
IPR001005	SANT/Myb domain	CDD	cd00167	SANT	coord: 16..62 e-value: 1.28984E-8 score: 49.111
None	No IPR available	GENE3D	G3DSA:1.10.10.60		coord: 7..68 e-value: 2.4E-19 score: 70.8
None	No IPR available	GENE3D	G3DSA:1.10.10.60		coord: 70..142 e-value: 5.1E-19 score: 70.1
None	No IPR available	PANTHER	PTHR10641:SF654	TRANSCRIPTION FACTOR RAX2-RELATED	coord: 1..224
None	No IPR available	PANTHER	PTHR10641	MYB-LIKE DNA-BINDING PROTEIN MYB	coord: 1..224
IPR017930	Myb domain	PROSITE	PS51294	HTH_MYB	coord: 63..117 score: 22.71
IPR017930	Myb domain	PROSITE	PS51294	HTH_MYB	coord: 9..62 score: 14.357
IPR009057	Homeobox-like domain superfamily	SUPERFAMILY	SSF46689	Homeodomain-like	coord: 11..109

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg12289	Cla017797	Watermelon (97103) v1	carwmB0439
Carg12289	Cla005093	Watermelon (97103) v1	carwmB0440
Carg12289	Csa3G740800	Cucumber (Chinese Long) v2	carcuB0474
Carg12289	Csa1G109320	Cucumber (Chinese Long) v2	carcuB0472
Carg12289	MELO3C004947	Melon (DHL92) v3.5.1	carmeB0449
Carg12289	ClCG03G002780	Watermelon (Charleston Gray)	carwcgB0436
Carg12289	ClCG10G020060	Watermelon (Charleston Gray)	carwcgB0432
Carg12289	CSPI01G13370	Wild cucumber (PI 183967)	carcpiB0477
Carg12289	CSPI03G33430	Wild cucumber (PI 183967)	carcpiB0479
Carg12289	Cucsa.292610	Cucumber (Gy14) v1	carcgyB0514
Carg12289	CmaCh13G002390	Cucurbita maxima (Rimu)	carcmaB0611
Carg12289	CmaCh01G005130	Cucurbita maxima (Rimu)	carcmaB0613
Carg12289	CmoCh13G002400	Cucurbita moschata (Rifu)	carcmoB0594
Carg12289	CmoCh01G005450	Cucurbita moschata (Rifu)	carcmoB0596
Carg12289	Lsi03G000120	Bottle gourd (USVL1VR-Ls)	carlsiB404
Carg12289	Cp4.1LG20g07170	Cucurbita pepo (Zucchini)	carcpeB0620
Carg12289	Cp4.1LG02g12550	Cucurbita pepo (Zucchini)	carcpeB0622
Carg12289	CsGy1G013470	Cucumber (Gy14) v2	carcgybB0449
Carg12289	CsGy3G031140	Cucumber (Gy14) v2	carcgybB0451
Carg12289	MELO3C004947.2	Melon (DHL92) v3.6.1	carmedB0452
Carg12289	CsaV3_3G036040	Cucumber (Chinese Long) v3	carcucB0476
Carg12289	CsaV3_1G013560	Cucumber (Chinese Long) v3	carcucB0474
Carg12289	Cla97C03G053830	Watermelon (97103) v2	carwmbB0467
Carg12289	Bhi11G001760	Wax gourd	carwgoB0528

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg12289	Carg02582	Silver-seed gourd	carcarB448

The following block(s) are covering this gene:


Gene	Organism	Block
Carg12289	Silver-seed gourd	carcarB450
Carg12289	Wax gourd	carwgoB0532