Carg11845 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg11845
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Calcium-binding EF-hand
Location	Cucurbita_argyrosperma_scaffold_036 : 422099 .. 422377 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGAATTCCAAAAGCAAAACTTACAAACGAGTTCTTGTTCCATTCTCCGAGGAACAACTAGCTAGCGTTTTCAAGAACCACGATGGAGATGGTGATGGAAAGCTAACAAAGGAGGAGTTGAAGCAAGCCTTCGACTATCTTGGTTCACGCTTTAGCTCGTTCAGAGTAGAAGAGGCACTACGTGCCGCTGATATCGACGGTGATGGTTTCATAAGCATGGCCGAGATGGACAAGCTAATTCAATATGCCAAAAGTCGTAAATACACTCTTTGTTAA

mRNA sequence

ATGGGGAATTCCAAAAGCAAAACTTACAAACGAGTTCTTGTTCCATTCTCCGAGGAACAACTAGCTAGCGTTTTCAAGAACCACGATGGAGATGGTGATGGAAAGCTAACAAAGGAGGAGTTGAAGCAAGCCTTCGACTATCTTGGTTCACGCTTTAGCTCGTTCAGAGTAGAAGAGGCACTACGTGCCGCTGATATCGACGGTGATGGTTTCATAAGCATGGCCGAGATGGACAAGCTAATTCAATATGCCAAAAGTCGTAAATACACTCTTTGTTAA

Coding sequence (CDS)

ATGGGGAATTCCAAAAGCAAAACTTACAAACGAGTTCTTGTTCCATTCTCCGAGGAACAACTAGCTAGCGTTTTCAAGAACCACGATGGAGATGGTGATGGAAAGCTAACAAAGGAGGAGTTGAAGCAAGCCTTCGACTATCTTGGTTCACGCTTTAGCTCGTTCAGAGTAGAAGAGGCACTACGTGCCGCTGATATCGACGGTGATGGTTTCATAAGCATGGCCGAGATGGACAAGCTAATTCAATATGCCAAAAGTCGTAAATACACTCTTTGTTAA

Protein sequence

MGNSKSKTYKRVLVPFSEEQLASVFKNHDGDGDGKLTKEELKQAFDYLGSRFSSFRVEEALRAADIDGDGFISMAEMDKLIQYAKSRKYTLC

Homology

[Close]

BLAST of Carg11845 vs. NCBI nr
Match: KGN62511.1 (hypothetical protein Csa_2G357340 [Cucumis sativus])

HSP 1 Score: 133.7 bits (335), Expect = 3.3e-28
Identity = 68/91 (74.73%), Postives = 74/91 (81.32%), Query Frame = 0


Query: 1  MGNSKSKTYKRVLVPFSEEQLASVFKNHDGDGDGKLTKEELKQAFDYLGSRFSSFRVEEA 60
          MGNSKSKTYKRVLVPFSEEQLA+VFKNHD DGDG LTKEELKQAF+YLGSRF +FRVEEA
Sbjct: 1  MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEA 60

Query: 61 LRAAXXXXXXXXXXXXXXKLIQYAKSRKYTL 92
          LRAA              KLI+YAK+RKYT+
Sbjct: 61 LRAADTNGDGVISMDEMGKLIKYAKTRKYTI 91

[Close]

BLAST of Carg11845 vs. NCBI nr
Match: XP_023538265.1 (probable calcium-binding protein CML15 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 114.4 bits (285), Expect = 2.1e-22
Identity = 58/85 (68.24%), Postives = 62/85 (72.94%), Query Frame = 0


Query: 1  MGNSKSKTYKRVLVPFSEEQLASVFKNHDGDGDGKLTKEELKQAFDYLGSRFSSFRVEEA 60
          MGNSKSKTYKRVLVP SEEQL  +FK+HD DGDGKL+KEELKQAF YLGSRFS+FR EEA
Sbjct: 1  MGNSKSKTYKRVLVPISEEQLLDIFKSHDQDGDGKLSKEELKQAFSYLGSRFSAFRAEEA 60

Query: 61 LRAAXXXXXXXXXXXXXXKLIQYAK 86
          LR                KLIQY K
Sbjct: 61 LRTVDDNHDGVVSMDEMNKLIQYTK 85

[Close]

BLAST of Carg11845 vs. NCBI nr
Match: POF10332.1 (calcium-binding allergen ole e 8 [Quercus suber])

HSP 1 Score: 83.6 bits (205), Expect = 3.9e-13
Identity = 40/87 (45.98%), Postives = 55/87 (63.22%), Query Frame = 0


Query: 5  KSKTYKRVLVPFSEEQLASVFKNHDGDGDGKLTKEELKQAFDYLGSRFSSFRVEEALRAA 64
          K   YK   V ++++QL +VFK+HD DGDGKL++EELK+AF YLGSR+ S+R E+ALR  
Sbjct: 4  KGTVYKPTSVLYTKDQLMNVFKSHDADGDGKLSREELKKAFKYLGSRWCSYRTEQALRCT 63

Query: 65 XXXXXXXXXXXXXXKLIQYAKSRKYTL 92
                        KL+ Y   R+YT+
Sbjct: 64 DTNDDGYIDEKELEKLVDYTLERQYTI 90

[Close]

BLAST of Carg11845 vs. NCBI nr
Match: POF10331.1 (putative calcium-binding protein cml26 [Quercus suber])

HSP 1 Score: 83.2 bits (204), Expect = 5.1e-13
Identity = 53/87 (60.92%), Postives = 71/87 (81.61%), Query Frame = 0


Query: 5  KSKTYKRVLVPFSEEQLASVFKNHDGDGDGKLTKEELKQAFDYLGSRFSSFRVEEALRAA 64
          K+  +KR  VPF+E+QL ++FK+HD DGDGKL+++ELK AF YLGSR+ ++R ++ALR A
Sbjct: 4  KAMAFKRTSVPFNEQQLLNIFKSHDVDGDGKLSRDELKNAFKYLGSRWDAYRTDQALRQA 63

Query: 65 XXXXXXXXXXXXXXKLIQYAKSRKYTL 92
          XXXXXXXXXXXXXX L++YA    YT+
Sbjct: 64 XXXXXXXXXXXXXXXLVEYALDCGYTV 90

[Close]

BLAST of Carg11845 vs. NCBI nr
Match: POE80202.1 (putative calcium-binding protein cml26 [Quercus suber])

HSP 1 Score: 77.8 bits (190), Expect = 2.1e-11
Identity = 33/58 (56.90%), Postives = 48/58 (82.76%), Query Frame = 0


Query: 5  KSKTYKRVLVPFSEEQLASVFKNHDGDGDGKLTKEELKQAFDYLGSRFSSFRVEEALR 63
          K+  +KR  VPF+E+QL ++FK+HD DGDGKL+++ELK AF YLGSR+ ++R ++ALR
Sbjct: 4  KAMAFKRTSVPFNEQQLLNIFKSHDVDGDGKLSRDELKNAFKYLGSRWDAYRTDQALR 61

[Close]

BLAST of Carg11845 vs. TAIR10
Match: AT5G12180.1 (calcium-dependent protein kinase 17)

HSP 1 Score: 40.0 bits (92), Expect = 9.0e-04
Identity = 23/67 (34.33%), Postives = 34/67 (50.75%), Query Frame = 0


Query: 1   MGNSKSKTYKRVLVPFSEEQ---LASVFKNHDGDGDGKLTKEELKQAFDYLGSRFSSFRV 60
           M N K    + +    SEE+   L  +FK  D D  G +T EEL+Q     G+R S + V
Sbjct: 356 MNNFKKVALRVIAGCLSEEEIMGLKEMFKGMDTDSSGTITLEELRQGLAKQGTRLSEYEV 415

Query: 61  EEALRAA 65
           ++ + AA
Sbjct: 416 QQLMEAA 422

[Close]

BLAST of Carg11845 vs. TAIR10
Match: AT5G19360.1 (calcium-dependent protein kinase 34)

HSP 1 Score: 40.0 bits (92), Expect = 9.0e-04
Identity = 23/67 (34.33%), Postives = 34/67 (50.75%), Query Frame = 0


Query: 1   MGNSKSKTYKRVLVPFSEEQ---LASVFKNHDGDGDGKLTKEELKQAFDYLGSRFSSFRV 60
           M N K    + +    SEE+   L  +FK  D D  G +T EEL+Q     G+R S + V
Sbjct: 351 MNNFKKVALRVIAGCLSEEEIMGLKEMFKGMDTDNSGTITLEELRQGLAKQGTRLSEYEV 410

Query: 61  EEALRAA 65
           ++ + AA
Sbjct: 411 QQLMEAA 417

[Close]

BLAST of Carg11845 vs. TrEMBL
Match: tr|A0A0A0LRB6|A0A0A0LRB6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357340 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 2.2e-28
Identity = 68/91 (74.73%), Postives = 74/91 (81.32%), Query Frame = 0


Query: 1  MGNSKSKTYKRVLVPFSEEQLASVFKNHDGDGDGKLTKEELKQAFDYLGSRFSSFRVEEA 60
          MGNSKSKTYKRVLVPFSEEQLA+VFKNHD DGDG LTKEELKQAF+YLGSRF +FRVEEA
Sbjct: 1  MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEA 60

Query: 61 LRAAXXXXXXXXXXXXXXKLIQYAKSRKYTL 92
          LRAA              KLI+YAK+RKYT+
Sbjct: 61 LRAADTNGDGVISMDEMGKLIKYAKTRKYTI 91

[Close]

BLAST of Carg11845 vs. TrEMBL
Match: tr|A0A2P4LY04|A0A2P4LY04_QUESU (Calcium-binding allergen ole e 8 OS=Quercus suber OX=58331 GN=CFP56_50060 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.6e-13
Identity = 40/87 (45.98%), Postives = 55/87 (63.22%), Query Frame = 0


Query: 5  KSKTYKRVLVPFSEEQLASVFKNHDGDGDGKLTKEELKQAFDYLGSRFSSFRVEEALRAA 64
          K   YK   V ++++QL +VFK+HD DGDGKL++EELK+AF YLGSR+ S+R E+ALR  
Sbjct: 4  KGTVYKPTSVLYTKDQLMNVFKSHDADGDGKLSREELKKAFKYLGSRWCSYRTEQALRCT 63

Query: 65 XXXXXXXXXXXXXXKLIQYAKSRKYTL 92
                        KL+ Y   R+YT+
Sbjct: 64 DTNDDGYIDEKELEKLVDYTLERQYTI 90

[Close]

BLAST of Carg11845 vs. TrEMBL
Match: tr|A0A2P4LYQ6|A0A2P4LYQ6_QUESU (Putative calcium-binding protein cml26 OS=Quercus suber OX=58331 GN=CFP56_50059 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 3.4e-13
Identity = 53/87 (60.92%), Postives = 71/87 (81.61%), Query Frame = 0


Query: 5  KSKTYKRVLVPFSEEQLASVFKNHDGDGDGKLTKEELKQAFDYLGSRFSSFRVEEALRAA 64
          K+  +KR  VPF+E+QL ++FK+HD DGDGKL+++ELK AF YLGSR+ ++R ++ALR A
Sbjct: 4  KAMAFKRTSVPFNEQQLLNIFKSHDVDGDGKLSRDELKNAFKYLGSRWDAYRTDQALRQA 63

Query: 65 XXXXXXXXXXXXXXKLIQYAKSRKYTL 92
          XXXXXXXXXXXXXX L++YA    YT+
Sbjct: 64 XXXXXXXXXXXXXXXLVEYALDCGYTV 90

[Close]

BLAST of Carg11845 vs. TrEMBL
Match: tr|A0A2P4JHG4|A0A2P4JHG4_QUESU (Putative calcium-binding protein cml26 OS=Quercus suber OX=58331 GN=CFP56_74189 PE=4 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.4e-11
Identity = 33/58 (56.90%), Postives = 48/58 (82.76%), Query Frame = 0


Query: 5  KSKTYKRVLVPFSEEQLASVFKNHDGDGDGKLTKEELKQAFDYLGSRFSSFRVEEALR 63
          K+  +KR  VPF+E+QL ++FK+HD DGDGKL+++ELK AF YLGSR+ ++R ++ALR
Sbjct: 4  KAMAFKRTSVPFNEQQLLNIFKSHDVDGDGKLSRDELKNAFKYLGSRWDAYRTDQALR 61

[Close]

BLAST of Carg11845 vs. TrEMBL
Match: tr|A0A0A0LP43|A0A0A0LP43_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357350 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.2e-11
Identity = 40/83 (48.19%), Postives = 49/83 (59.04%), Query Frame = 0


Query: 1  MGNSKSKTYKRVLVPFSEEQLASVFKNHDGDGDGKLTKEELKQAFDYLGSRFSSFRVEEA 60
          MG S    +K      +E ++A VFKNHD DGDG+LTKEELK AF YLG +F++FRV +A
Sbjct: 1  MGTSNGLIFKCFPASLTEFEVAKVFKNHDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQA 60

Query: 61 LRAAXXXXXXXXXXXXXXKLIQY 84
          L AA              KLI Y
Sbjct: 61 LHAADDNCDDVISFEEMSKLIMY 83

The following BLAST results are available for this feature:

BLAST of Carg11845 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN62511.1	3.3e-28	74.73	hypothetical protein Csa_2G357340 [Cucumis sativus]	[more]
XP_023538265.1	2.1e-22	68.24	probable calcium-binding protein CML15 [Cucurbita pepo subsp. pepo]	[more]
POF10332.1	3.9e-13	45.98	calcium-binding allergen ole e 8 [Quercus suber]	[more]
POF10331.1	5.1e-13	60.92	putative calcium-binding protein cml26 [Quercus suber]	[more]
POE80202.1	2.1e-11	56.90	putative calcium-binding protein cml26 [Quercus suber]	[more]

BLAST of Carg11845 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G12180.1	9.0e-04	34.33	calcium-dependent protein kinase 17	[more]
AT5G19360.1	9.0e-04	34.33	calcium-dependent protein kinase 34	[more]

BLAST of Carg11845 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Carg11845 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LRB6\|A0A0A0LRB6_CUCSA	2.2e-28	74.73	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357340 PE=4 SV=1	[more]
tr\|A0A2P4LY04\|A0A2P4LY04_QUESU	2.6e-13	45.98	Calcium-binding allergen ole e 8 OS=Quercus suber OX=58331 GN=CFP56_50060 PE=4 S...	[more]
tr\|A0A2P4LYQ6\|A0A2P4LYQ6_QUESU	3.4e-13	60.92	Putative calcium-binding protein cml26 OS=Quercus suber OX=58331 GN=CFP56_50059 ...	[more]
tr\|A0A2P4JHG4\|A0A2P4JHG4_QUESU	1.4e-11	56.90	Putative calcium-binding protein cml26 OS=Quercus suber OX=58331 GN=CFP56_74189 ...	[more]
tr\|A0A0A0LP43\|A0A0A0LP43_CUCSA	3.2e-11	48.19	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357350 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS
IPR002048	EF_hand_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg11845-RA	Carg11845-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 56..84 e-value: 0.71 score: 18.9 coord: 20..48 e-value: 0.36 score: 19.9
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 18..81 e-value: 5.1E-12 score: 46.0
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 52..87 score: 10.943
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 16..51 score: 14.486
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 20..82 e-value: 2.30648E-12 score: 56.4021
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 1..89 e-value: 1.8E-15 score: 59.3
None	No IPR available	PANTHER	PTHR44455:SF5	SUBFAMILY NOT NAMED	coord: 12..86
None	No IPR available	PANTHER	PTHR44455	FAMILY NOT NAMED	coord: 12..86
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 65..77
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 29..41
IPR011992	EF-hand domain pair	SUPERFAMILY	SSF47473	EF-hand	coord: 8..81

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg11845	Cla022560	Watermelon (97103) v1	carwmB0172
Carg11845	Cla020682	Watermelon (97103) v1	carwmB0173
Carg11845	Csa4G639730	Cucumber (Chinese Long) v2	carcuB0204
Carg11845	Csa2G357340	Cucumber (Chinese Long) v2	carcuB0202
Carg11845	MELO3C016746	Melon (DHL92) v3.5.1	carmeB0187
Carg11845	MELO3C010868	Melon (DHL92) v3.5.1	carmeB0186
Carg11845	ClCG08G015790	Watermelon (Charleston Gray)	carwcgB0178
Carg11845	ClCG05G019800	Watermelon (Charleston Gray)	carwcgB0175
Carg11845	CSPI02G19130	Wild cucumber (PI 183967)	carcpiB0209
Carg11845	CSPI04G24010	Wild cucumber (PI 183967)	carcpiB0211
Carg11845	Cucsa.232870	Cucumber (Gy14) v1	carcgyB0222
Carg11845	CmaCh09G002210	Cucurbita maxima (Rimu)	carcmaB0247
Carg11845	CmaCh12G005380	Cucurbita maxima (Rimu)	carcmaB0249
Carg11845	CmaCh01G018470	Cucurbita maxima (Rimu)	carcmaB0251
Carg11845	CmaCh05G001890	Cucurbita maxima (Rimu)	carcmaB0256
Carg11845	CmoCh12G004770	Cucurbita moschata (Rifu)	carcmoB0257
Carg11845	CmoCh05G001880	Cucurbita moschata (Rifu)	carcmoB0263
Carg11845	CmoCh01G019050	Cucurbita moschata (Rifu)	carcmoB0259
Carg11845	CmoCh09G002130	Cucurbita moschata (Rifu)	carcmoB0255
Carg11845	Lsi04G015700	Bottle gourd (USVL1VR-Ls)	carlsiB162
Carg11845	Lsi08G014600	Bottle gourd (USVL1VR-Ls)	carlsiB164
Carg11845	Cp4.1LG07g05260	Cucurbita pepo (Zucchini)	carcpeB0268
Carg11845	Cp4.1LG06g01580	Cucurbita pepo (Zucchini)	carcpeB0267
Carg11845	Cp4.1LG02g07030	Cucurbita pepo (Zucchini)	carcpeB0265
Carg11845	Cp4.1LG11g01520	Cucurbita pepo (Zucchini)	carcpeB0263
Carg11845	CsGy2G019050	Cucumber (Gy14) v2	carcgybB0195
Carg11845	CsGy4G022320	Cucumber (Gy14) v2	carcgybB0197
Carg11845	MELO3C016746.2	Melon (DHL92) v3.6.1	carmedB0191
Carg11845	MELO3C010868.2	Melon (DHL92) v3.6.1	carmedB0190
Carg11845	CsaV3_4G034250	Cucumber (Chinese Long) v3	carcucB0211
Carg11845	CsaV3_2G027840	Cucumber (Chinese Long) v3	carcucB0209
Carg11845	Cla97C05G101420	Watermelon (97103) v2	carwmbB0187
Carg11845	Cla97C08G159390	Watermelon (97103) v2	carwmbB0190
Carg11845	Bhi04G000317	Wax gourd	carwgoB0231
Carg11845	Bhi09G002738	Wax gourd	carwgoB0236

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg11845	Carg26146	Silver-seed gourd	carcarB090
Carg11845	Carg08773	Silver-seed gourd	carcarB119
Carg11845	Carg10597	Silver-seed gourd	carcarB224

The following block(s) are covering this gene:


Gene	Organism	Block
Carg11845	Wax gourd	carwgoB0235
Carg11845	Silver-seed gourd	carcarB218
Carg11845	Silver-seed gourd	carcarB225
Carg11845	Cucumber (Gy14) v2	carcgybB0198
Carg11845	Cucumber (Gy14) v1	carcgyB0219
Carg11845	Cucurbita maxima (Rimu)	carcmaB0254
Carg11845	Cucurbita maxima (Rimu)	carcmaB0257
Carg11845	Cucurbita moschata (Rifu)	carcmoB0262
Carg11845	Cucurbita moschata (Rifu)	carcmoB0264
Carg11845	Cucurbita pepo (Zucchini)	carcpeB0261
Carg11845	Cucurbita pepo (Zucchini)	carcpeB0264
Carg11845	Wild cucumber (PI 183967)	carcpiB0212
Carg11845	Cucumber (Chinese Long) v2	carcuB0205
Carg11845	Cucumber (Chinese Long) v3	carcucB0212
Carg11845	Bottle gourd (USVL1VR-Ls)	carlsiB161
Carg11845	Melon (DHL92) v3.5.1	carmeB0188
Carg11845	Melon (DHL92) v3.6.1	carmedB0192
Carg11845	Watermelon (Charleston Gray)	carwcgB0173
Carg11845	Watermelon (97103) v1	carwmB0176
Carg11845	Watermelon (97103) v2	carwmbB0185