Cla020682 (gene) Watermelon (97103) v1

Overview
NameCla020682
Typegene
OrganismCitrullus. lanatus (Watermelon (97103) v1)
DescriptionPolcalcin Bet v 4 (AHRD V1 ***- POLC4_BETPN); contains Interpro domain(s) IPR011992 EF-Hand type
LocationChr5 : 27749131 .. 27749403 (-)
Genome Viewer
Gene Expression
   



Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAGGAAAAGCAAAACGTACCATCCCAGGGCGAAGCTATCAAGAGAGCAGTTACTTGTAATCTTCAGAAACCACGACAGCGACAAGGACGGCCGCCTCAGCACCACCGAACTCACCGAAGCCTTCCGATATATCGGCGCCTTTCTCCCGTCCTACAGAGCTCGCCACGCTCTTTTTCATTCCGATTCCGACAGAGATGGATTTATCGGAGAGAAGGATTTCGAGTTGCTCCTCAATTATGCCGAGCAACGCCAATATACCATCATTTGA

mRNA sequence

ATGGCGAGGAAAAGCAAAACGTACCATCCCAGGGCGAAGCTATCAAGAGAGCAGTTACTTGTAATCTTCAGAAACCACGACAGCGACAAGGACGGCCGCCTCAGCACCACCGAACTCACCGAAGCCTTCCGATATATCGGCGCCTTTCTCCCGTCCTACAGAGCTCGCCACGCTCTTTTTCATTCCGATTCCGACAGAGATGGATTTATCGGAGAGAAGGATTTCGAGTTGCTCCTCAATTATGCCGAGCAACGCCAATATACCATCATTTGA

Coding sequence (CDS)

ATGGCGAGGAAAAGCAAAACGTACCATCCCAGGGCGAAGCTATCAAGAGAGCAGTTACTTGTAATCTTCAGAAACCACGACAGCGACAAGGACGGCCGCCTCAGCACCACCGAACTCACCGAAGCCTTCCGATATATCGGCGCCTTTCTCCCGTCCTACAGAGCTCGCCACGCTCTTTTTCATTCCGATTCCGACAGAGATGGATTTATCGGAGAGAAGGATTTCGAGTTGCTCCTCAATTATGCCGAGCAACGCCAATATACCATCATTTGA

Protein sequence

MARKSKTYHPRAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSDRDGFIGEKDFELLLNYAEQRQYTII
Homology
[Close]
BLAST of Cla020682 vs. TrEMBL
Match: A0A0A0KZR6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G639730 PE=4 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 2.5e-39
Identity = 81/90 (90.00%), Postives = 87/90 (96.67%), Query Frame = 1 

Query: 1  MARKSKTYHPRAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALF 60
          M+RKSK Y+PRAKLSREQLL+IFRNHDSDKDGRLSTTELTEAFRYIGAFLP+YRARHAL 
Sbjct: 1  MSRKSKNYYPRAKLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPTYRARHALL 60

Query: 61 HSDSDRDGFIGEKDFELLLNYAEQRQYTII 91
          H+DSDRDGFIGEKDFE L+NYAEQRQYTII
Sbjct: 61 HADSDRDGFIGEKDFESLVNYAEQRQYTII 90

[Close]
BLAST of Cla020682 vs. TrEMBL
Match: A5AWP8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_023174 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 3.4e-12
Identity = 37/84 (44.05%), Postives = 57/84 (67.86%), Query Frame = 1 

Query: 6  KTYHPRAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSD 65
          KT   ++ L  +QL  IF+ HD++ DG+L+  EL +AF+++GA +P +RA  AL H+D+D
Sbjct: 7  KTIPSKSPLPEQQLKKIFKQHDTNGDGQLNKAELKQAFQHLGALIPGWRAHRALHHADTD 66

Query: 66 RDGFIGEKDFELLLNYAEQRQYTI 90
           DG I EK+ + L+ YA +  YTI
Sbjct: 67 GDGCISEKEMKDLVEYAAKFGYTI 90

[Close]
BLAST of Cla020682 vs. TrEMBL
Match: W9RZZ8_9ROSA (Putative calcium-binding protein CML27 OS=Morus notabilis GN=L484_006332 PE=4 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 4.4e-12
Identity = 37/77 (48.05%), Postives = 51/77 (66.23%), Query Frame = 1 

Query: 11  RAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSDRDGFI 70
           +A  + EQL  +F+ HD++KDG LS  ELT+AF  +G+F PS+RA  AL H D +RDGF+
Sbjct: 24  KAPYNEEQLKDVFKKHDTNKDGLLSREELTKAFSSLGSFFPSWRASRALSHVDKNRDGFV 83

Query: 71  GEKDFELLLNYAEQRQY 88
            E +F  L+ Y  Q  Y
Sbjct: 84  DENEFSDLVRYVAQLGY 100

[Close]
BLAST of Cla020682 vs. TrEMBL
Match: A0A0A0LRC1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.7e-11
Identity = 35/77 (45.45%), Postives = 53/77 (68.83%), Query Frame = 1 

Query: 11 RAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSDRDGFI 70
          R +LSRE +  IFR HD D DGRLS  E+ +AF   G+ LP Y+A + + H+D + DGFI
Sbjct: 7  RLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFI 66

Query: 71 GEKDFELLLNYAEQRQY 88
           E++ + L++YA++ Q+
Sbjct: 67 EEEELDKLVDYAQKFQH 83

[Close]
BLAST of Cla020682 vs. TrEMBL
Match: A0A0A0LKW4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 4.9e-11
Identity = 35/78 (44.87%), Postives = 52/78 (66.67%), Query Frame = 1 

Query: 13 KLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSDRDGFIGE 72
          KL +E++  +FR HD + DGRLS  EL+ AF   GAF P YRA   LF +D D DGFI E
Sbjct: 14 KLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGFISE 73

Query: 73 KDFELLLNYAEQRQYTII 91
          ++ + +++YA + +Y ++
Sbjct: 74 QELDKVVDYAIKCKYNLL 91

[Close]
BLAST of Cla020682 vs. NCBI nr
Match: gi|700200027|gb|KGN55185.1| (hypothetical protein Csa_4G639730 [Cucumis sativus])

HSP 1 Score: 169.1 bits (427), Expect = 3.6e-39
Identity = 81/90 (90.00%), Postives = 87/90 (96.67%), Query Frame = 1 

Query: 1  MARKSKTYHPRAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALF 60
          M+RKSK Y+PRAKLSREQLL+IFRNHDSDKDGRLSTTELTEAFRYIGAFLP+YRARHAL 
Sbjct: 1  MSRKSKNYYPRAKLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPTYRARHALL 60

Query: 61 HSDSDRDGFIGEKDFELLLNYAEQRQYTII 91
          H+DSDRDGFIGEKDFE L+NYAEQRQYTII
Sbjct: 61 HADSDRDGFIGEKDFESLVNYAEQRQYTII 90

[Close]
BLAST of Cla020682 vs. NCBI nr
Match: gi|147866710|emb|CAN78405.1| (hypothetical protein VITISV_023174 [Vitis vinifera])

HSP 1 Score: 79.0 bits (193), Expect = 4.8e-12
Identity = 37/84 (44.05%), Postives = 57/84 (67.86%), Query Frame = 1 

Query: 6  KTYHPRAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSD 65
          KT   ++ L  +QL  IF+ HD++ DG+L+  EL +AF+++GA +P +RA  AL H+D+D
Sbjct: 7  KTIPSKSPLPEQQLKKIFKQHDTNGDGQLNKAELKQAFQHLGALIPGWRAHRALHHADTD 66

Query: 66 RDGFIGEKDFELLLNYAEQRQYTI 90
           DG I EK+ + L+ YA +  YTI
Sbjct: 67 GDGCISEKEMKDLVEYAAKFGYTI 90

[Close]
BLAST of Cla020682 vs. NCBI nr
Match: gi|703145697|ref|XP_010108601.1| (putative calcium-binding protein CML27 [Morus notabilis])

HSP 1 Score: 78.6 bits (192), Expect = 6.3e-12
Identity = 37/77 (48.05%), Postives = 51/77 (66.23%), Query Frame = 1 

Query: 11  RAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSDRDGFI 70
           +A  + EQL  +F+ HD++KDG LS  ELT+AF  +G+F PS+RA  AL H D +RDGF+
Sbjct: 24  KAPYNEEQLKDVFKKHDTNKDGLLSREELTKAFSSLGSFFPSWRASRALSHVDKNRDGFV 83

Query: 71  GEKDFELLLNYAEQRQY 88
            E +F  L+ Y  Q  Y
Sbjct: 84  DENEFSDLVRYVAQLGY 100

[Close]
BLAST of Cla020682 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 76.6 bits (187), Expect = 2.4e-11
Identity = 35/77 (45.45%), Postives = 53/77 (68.83%), Query Frame = 1 

Query: 11  RAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSDRDGFI 70
           R +LSRE +  IFR HD D DGRLS  E+ +AF   G+ LP Y+A + + H+D + DGFI
Sbjct: 88  RLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFI 147

Query: 71  GEKDFELLLNYAEQRQY 88
            E++ + L++YA++ Q+
Sbjct: 148 EEEELDKLVDYAQKFQH 164

[Close]
BLAST of Cla020682 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 72.4 bits (176), Expect = 4.5e-10
Identity = 34/70 (48.57%), Postives = 47/70 (67.14%), Query Frame = 1 

Query: 13 KLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSDRDGFIGE 72
          KL +E++  +FR HD + DGRLS  EL+ AF   GAF P YRA   LF +D D DGFI E
Sbjct: 14 KLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGFISE 73

Query: 73 KDFELLLNYA 83
          ++ + +++YA
Sbjct: 74 QELDKVVDYA 83


HSP 2 Score: 76.6 bits (187), Expect = 2.4e-11
Identity = 35/77 (45.45%), Postives = 53/77 (68.83%), Query Frame = 1 

Query: 11 RAKLSREQLLVIFRNHDSDKDGRLSTTELTEAFRYIGAFLPSYRARHALFHSDSDRDGFI 70
          R +LSRE +  IFR HD D DGRLS  E+ +AF   G+ LP Y+A + + H+D + DGFI
Sbjct: 7  RLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFI 66

Query: 71 GEKDFELLLNYAEQRQY 88
           E++ + L++YA++ Q+
Sbjct: 67 EEEELDKLVDYAQKFQH 83

The following BLAST results are available for this feature:
BLAST of Cla020682 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KZR6_CUCSA2.5e-3990.00Uncharacterized protein OS=Cucumis sativus GN=Csa_4G639730 PE=4 SV=1[more]
A5AWP8_VITVI3.4e-1244.05Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_023174 PE=4 SV=1[more]
W9RZZ8_9ROSA4.4e-1248.05Putative calcium-binding protein CML27 OS=Morus notabilis GN=L484_006332 PE=4 SV... [more]
A0A0A0LRC1_CUCSA1.7e-1145.45Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1[more]
A0A0A0LKW4_CUCSA4.9e-1144.87Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1[more]
BLAST of Cla020682 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700200027|gb|KGN55185.1|3.6e-3990.00hypothetical protein Csa_4G639730 [Cucumis sativus][more]
gi|147866710|emb|CAN78405.1|4.8e-1244.05hypothetical protein VITISV_023174 [Vitis vinifera][more]
gi|703145697|ref|XP_010108601.1|6.3e-1248.05putative calcium-binding protein CML27 [Morus notabilis][more]
gi|449470198|ref|XP_004152805.1|2.4e-1145.45PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus][more]
gi|449470198|ref|XP_004152805.1|4.5e-1048.57PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Unigene
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
WMU29773watermelon unigene v2 vs TrEMBLtranscribed_cluster
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla020682Cla020682.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
WMU29773WMU29773transcribed_cluster


InterPro
Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13405EF-hand_6coord: 20..45
score: 4.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 63..85
score: 6.758coord: 14..49
score: 12
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 16..79
score: 2.2
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 13..81
score: 7.29
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 27..39
score: -coord: 63..75
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 14..82
score: 3.5
NoneNo IPR availablePANTHERPTHR10891:SF592CALCIUM-BINDING PROTEIN CML38-RELATEDcoord: 14..82
score: 3.5

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cla020682Cla97C05G101420Watermelon (97103) v2wmwmbB207
Cla020682Cla97C08G159380Watermelon (97103) v2wmwmbB222
Cla020682Csa4G639730Cucumber (Chinese Long) v2cuwmB319
Cla020682MELO3C016745Melon (DHL92) v3.5.1mewmB527
Cla020682ClCG08G015800Watermelon (Charleston Gray)wcgwmB412
Cla020682ClCG05G019800Watermelon (Charleston Gray)wcgwmB285
Cla020682CSPI02G19280Wild cucumber (PI 183967)cpiwmB129
Cla020682CSPI04G24010Wild cucumber (PI 183967)cpiwmB324
Cla020682Cucsa.232870Cucumber (Gy14) v1cgywmB434
Cla020682CmaCh05G001890Cucurbita maxima (Rimu)cmawmB758
Cla020682CmaCh01G018470Cucurbita maxima (Rimu)cmawmB440
Cla020682CmaCh12G005410Cucurbita maxima (Rimu)cmawmB159
Cla020682CmoCh01G019060Cucurbita moschata (Rifu)cmowmB434
Cla020682CmoCh09G002130Cucurbita moschata (Rifu)cmowmB018
Cla020682Lsi04G015710Bottle gourd (USVL1VR-Ls)lsiwmB316
Cla020682Lsi08G014600Bottle gourd (USVL1VR-Ls)lsiwmB480
Cla020682Cp4.1LG07g05260Cucurbita pepo (Zucchini)cpewmB795
Cla020682Cp4.1LG11g01520Cucurbita pepo (Zucchini)cpewmB108
Cla020682CsGy2G019150Cucumber (Gy14) v2cgybwmB119
Cla020682MELO3C010876.2Melon (DHL92) v3.6.1medwmB311
Cla020682MELO3C016745.2Melon (DHL92) v3.6.1medwmB512
Cla020682Carg11845Silver-seed gourdcarwmB0173
Cla020682Carg10597Silver-seed gourdcarwmB0769
Cla020682Carg08773Silver-seed gourdcarwmB0102
Cla020682CsaV3_4G034250Cucumber (Chinese Long) v3cucwmB332
Cla020682CsaV3_2G027970Cucumber (Chinese Long) v3cucwmB138
Cla020682Bhi04G000312Wax gourdwgowmB329
Cla020682Bhi09G002739Wax gourdwgowmB623
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cla020682Cla022561Watermelon (97103) v1wmwmB059
The following block(s) are covering this gene:
GeneOrganismBlock
Cla020682Cucumber (Gy14) v1cgywmB196
Cla020682Cucurbita maxima (Rimu)cmawmB026
Cla020682Cucurbita moschata (Rifu)cmowmB145
Cla020682Cucurbita moschata (Rifu)cmowmB753
Cla020682Melon (DHL92) v3.5.1mewmB317
Cla020682Cucumber (Chinese Long) v2cuwmB126
Cla020682Cucurbita pepo (Zucchini)cpewmB559
Cla020682Cucurbita pepo (Zucchini)cpewmB765
Cla020682Cucumber (Gy14) v2cgybwmB295