Cucsa.232870 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.232870
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Calcium-binding EF-hand family protein, putative
Location	scaffold01989 : 647568 .. 648151 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAACGGCGAAGGTTGCGAAGTCGTCTAGGTTTCATCTAAAATGACTCGTCAATTATATGACAAGAAGAAATCAGGGTACAAGTTGCAAAAAGAAGAAATGAAAGAAATGTTTAGGGAGCATGATTTCAATGGCGACGGTCGCTTGAGCATTAAAGAATTGTCGAGAGCATTCGGCCAATTTGGCGCATTCTTCCCTCTCTACCGTGCAGCTTTTGGGTTGTTCCTTGCAGATGATGACGGCGATGGATTTATCAGTGAGCAAGAACTAGATAAAGTGGTAGATTATGCTATTAAATGTAAGTATAACCTACTTTAGTTATCTTTTCCATCCTGCTGTGGAAGGTTCCCTAGTGAGTGTGAGTTGTTGATGTAAAAAAATAATATACAGAAAGGTAATTTGTGTGTTCAAGAGATACCTTCCCCTATTATTGTAAAATGCCTTTCCTGATGGCTGATGCGAAGATGATGATGATAGCATTTCCAATAAAAATAAATTTTATGCTTCATTCTTGCTCTTGCATGATATGATAACAATTTTCTAAATATATGTATATTGTTACAAAATAATAGACGCACTTTATTT

mRNA sequence

AAAACGGCGAAGGTTGCGAAGTCGTCTAGGTTTCATCTAAAATGACTCGTCAATTATATGACAAGAAGAAATCAGGGTACAAGTTGCAAAAAGAAGAAATGAAAGAAATGTTTAGGGAGCATGATTTCAATGGCGACGGTCGCTTGAGCATTAAAGAATTGTCGAGAGCATTCGGCCAATTTGGCGCATTCTTCCCTCTCTACCGTGCAGCTTTTGGGTTGTTCCTTGCAGATGATGACGGCGATGGATTTATCAGTGAGCAAGAACTAGATAAAGTGGTAGATTATGCTATTAAATGTAAGTATAACCTACTTTAGTTATCTTTTCCATCCTGCTGTGGAAGGTTCCCTAGTGAGTGTGAGTTGTTGATGTAAAAAAATAATATACAGAAAGGTAATTTGTGTGTTCAAGAGATACCTTCCCCTATTATTGTAAAATGCCTTTCCTGATGGCTGATGCGAAGATGATGATGATAGCATTTCCAATAAAAATAAATTTTATGCTTCATTCTTGCTCTTGCATGATATGATAACAATTTTCTAAATATATGTATATTGTTACAAAATAATAGACGCACTTTATTT

Coding sequence (CDS)

ATGACTCGTCAATTATATGACAAGAAGAAATCAGGGTACAAGTTGCAAAAAGAAGAAATGAAAGAAATGTTTAGGGAGCATGATTTCAATGGCGACGGTCGCTTGAGCATTAAAGAATTGTCGAGAGCATTCGGCCAATTTGGCGCATTCTTCCCTCTCTACCGTGCAGCTTTTGGGTTGTTCCTTGCAGATGATGACGGCGATGGATTTATCAGTGAGCAAGAACTAGATAAAGTGGTAGATTATGCTATTAAATGTAAGTATAACCTACTTTAG

Protein sequence

MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGFISEQELDKVVDYAIKCKYNLL*

Homology

[Close]

BLAST of Cucsa.232870 vs. TrEMBL
Match: A0A0A0LKW4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 4.0e-45
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 1


Query: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60
          MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL
Sbjct: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60

Query: 61 FLADDDGDGFISEQELDKVVDYAIKCKYNLL 92
          FLADDDGDGFISEQELDKVVDYAIKCKYNLL
Sbjct: 61 FLADDDGDGFISEQELDKVVDYAIKCKYNLL 91

[Close]

BLAST of Cucsa.232870 vs. TrEMBL
Match: A0A0A0LRC1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.6e-17
Identity = 42/75 (56.00%), Postives = 61/75 (81.33%), Query Frame = 1


Query: 14 KLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGFISE 73
          +L +E++KE+FREHD +GDGRLS+ E+ +AFG FG+  P Y+A +G+  AD++GDGFI E
Sbjct: 9  QLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFIEE 68

Query: 74 QELDKVVDYAIKCKY 89
          +ELDK+VDYA K ++
Sbjct: 69 EELDKLVDYAQKFQH 83

[Close]

BLAST of Cucsa.232870 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 5.7e-15
Identity = 40/73 (54.79%), Postives = 52/73 (71.23%), Query Frame = 1


Query: 11 SGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGF 70
          S +   +EEMKE+FREHD +GDG LSI EL +A G  G   P Y+A +G+   D++GDG 
Sbjct: 7  SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66

Query: 71 ISEQELDKVVDYA 84
          ISE ELDK++DYA
Sbjct: 67 ISEDELDKLIDYA 79

[Close]

BLAST of Cucsa.232870 vs. TrEMBL
Match: A0A0A0KMQ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 6.3e-14
Identity = 39/82 (47.56%), Postives = 56/82 (68.29%), Query Frame = 1


Query: 4   QLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLA 63
           ++ D+K S   L +E +KE+F+ HD N DG L+  EL++AF   G+ FP Y+A +G+  A
Sbjct: 85  RVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLGSMFPFYKACYGMVYA 144

Query: 64  DDDGDGFISEQELDKVVDYAIK 86
           D + DG ISE ELDK++DYA K
Sbjct: 145 DANEDGLISEAELDKLIDYASK 166

[Close]

BLAST of Cucsa.232870 vs. TrEMBL
Match: A0A0A0LRC8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359950 PE=4 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 5.3e-13
Identity = 41/88 (46.59%), Postives = 58/88 (65.91%), Query Frame = 1


Query: 3   RQLYDK-----KKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAA 62
           +QL DK     K+S   L +E++KE+F+ HD N DG LSI+EL++AF   G+  P  +A 
Sbjct: 79  KQLLDKAQGSSKQSNVPLSREQIKEIFKYHDSNKDGFLSIRELTKAFSSMGSIVPFCKAR 138

Query: 63  FGLFLADDDGDGFISEQELDKVVDYAIK 86
           + +  AD D DG +SE ELDK++DYA K
Sbjct: 139 YAMAYADVDEDGLVSEAELDKLIDYAHK 166

[Close]

BLAST of Cucsa.232870 vs. NCBI nr
Match: gi|700207396|gb|KGN62515.1| (hypothetical protein Csa_2G358860 [Cucumis sativus])

HSP 1 Score: 188.3 bits (477), Expect = 5.8e-45
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 1


Query: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60
          MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL
Sbjct: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60

Query: 61 FLADDDGDGFISEQELDKVVDYAIKCKYNLL 92
          FLADDDGDGFISEQELDKVVDYAIKCKYNLL
Sbjct: 61 FLADDDGDGFISEQELDKVVDYAIKCKYNLL 91

[Close]

BLAST of Cucsa.232870 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 174.9 bits (442), Expect = 6.6e-41
Identity = 85/85 (100.00%), Postives = 85/85 (100.00%), Query Frame = 1


Query: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60
          MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL
Sbjct: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60

Query: 61 FLADDDGDGFISEQELDKVVDYAIK 86
          FLADDDGDGFISEQELDKVVDYAIK
Sbjct: 61 FLADDDGDGFISEQELDKVVDYAIK 85

[Close]

BLAST of Cucsa.232870 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 98.6 bits (244), Expect = 6.0e-18
Identity = 44/83 (53.01%), Postives = 64/83 (77.11%), Query Frame = 1


Query: 6   YDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADD 65
           Y  K +  +L +E++KE+FREHD +GDGRLS+ E+ +AFG FG+  P Y+A +G+  AD+
Sbjct: 82  YAIKSTRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 141

Query: 66  DGDGFISEQELDKVVDYAIKCKY 89
           +GDGFI E+ELDK+VDYA K ++
Sbjct: 142 NGDGFIEEEELDKLVDYAQKFQH 164

HSP 2 Score: 96.7 bits (239), Expect = 2.3e-17
Identity = 42/75 (56.00%), Postives = 61/75 (81.33%), Query Frame = 1


Query: 14 KLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGFISE 73
          +L +E++KE+FREHD +GDGRLS+ E+ +AFG FG+  P Y+A +G+  AD++GDGFI E
Sbjct: 9  QLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFIEE 68

Query: 74 QELDKVVDYAIKCKY 89
          +ELDK+VDYA K ++
Sbjct: 69 EELDKLVDYAQKFQH 83

[Close]

BLAST of Cucsa.232870 vs. NCBI nr
Match: gi|700207406|gb|KGN62525.1| (hypothetical protein Csa_2G359940 [Cucumis sativus])

HSP 1 Score: 88.2 bits (217), Expect = 8.2e-15
Identity = 40/73 (54.79%), Postives = 52/73 (71.23%), Query Frame = 1


Query: 11 SGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGF 70
          S +   +EEMKE+FREHD +GDG LSI EL +A G  G   P Y+A +G+   D++GDG 
Sbjct: 7  SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66

Query: 71 ISEQELDKVVDYA 84
          ISE ELDK++DYA
Sbjct: 67 ISEDELDKLIDYA 79

[Close]

BLAST of Cucsa.232870 vs. NCBI nr
Match: gi|700194510|gb|KGN49687.1| (hypothetical protein Csa_5G067410 [Cucumis sativus])

HSP 1 Score: 84.7 bits (208), Expect = 9.0e-14
Identity = 39/82 (47.56%), Postives = 56/82 (68.29%), Query Frame = 1


Query: 4   QLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLA 63
           ++ D+K S   L +E +KE+F+ HD N DG L+  EL++AF   G+ FP Y+A +G+  A
Sbjct: 85  RVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLGSMFPFYKACYGMVYA 144

Query: 64  DDDGDGFISEQELDKVVDYAIK 86
           D + DG ISE ELDK++DYA K
Sbjct: 145 DANEDGLISEAELDKLIDYASK 166

The following BLAST results are available for this feature:

BLAST of Cucsa.232870 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LKW4_CUCSA	4.0e-45	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1	[more]
A0A0A0LRC1_CUCSA	1.6e-17	56.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1	[more]
A0A0A0LKX1_CUCSA	5.7e-15	54.79	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1	[more]
A0A0A0KMQ1_CUCSA	6.3e-14	47.56	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1	[more]
A0A0A0LRC8_CUCSA	5.3e-13	46.59	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359950 PE=4 SV=1	[more]

BLAST of Cucsa.232870 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700207396\|gb\|KGN62515.1\|	5.8e-45	100.00	hypothetical protein Csa_2G358860 [Cucumis sativus]	[more]
gi\|449470198\|ref\|XP_004152805.1\|	6.6e-41	100.00	PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus]	[more]
gi\|449470198\|ref\|XP_004152805.1\|	6.0e-18	53.01	PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus]	[more]
gi\|700207406\|gb\|KGN62525.1\|	8.2e-15	54.79	hypothetical protein Csa_2G359940 [Cucumis sativus]	[more]
gi\|700194510\|gb\|KGN49687.1\|	9.0e-14	47.56	hypothetical protein Csa_5G067410 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.232870.1	Cucsa.232870.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 18..75 score: 1.
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 53..83 score: 80.0coord: 19..47 score: 2.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 15..50 score: 14.541coord: 62..86 score: 7
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 10..80 score: 1.2
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 12..77 score: 1.36
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 64..76 score: -coord: 28..40 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 15..84 score: 1.8
None	No IPR available	PANTHER	PTHR10891:SF592	CALCIUM-BINDING PROTEIN CML38-RELATED	coord: 15..84 score: 1.8

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.232870	Cla020682	Watermelon (97103) v1	cgywmB434
Cucsa.232870	Csa2G358860	Cucumber (Chinese Long) v2	cgycuB326
Cucsa.232870	Csa4G639730	Cucumber (Chinese Long) v2	cgycuB328
Cucsa.232870	MELO3C010869	Melon (DHL92) v3.5.1	cgymeB389
Cucsa.232870	ClCG05G019800	Watermelon (Charleston Gray)	cgywcgB406
Cucsa.232870	CSPI02G19170	Wild cucumber (PI 183967)	cgycpiB341
Cucsa.232870	CSPI04G24010	Wild cucumber (PI 183967)	cgycpiB343
Cucsa.232870	Lsi08G014620	Bottle gourd (USVL1VR-Ls)	cgylsiB379
Cucsa.232870	MELO3C010869.2	Melon (DHL92) v3.6.1	cgymedB387
Cucsa.232870	CsaV3_2G027870	Cucumber (Chinese Long) v3	cgycucB347
Cucsa.232870	Bhi04G000306	Wax gourd	cgywgoB458
Cucsa.232870	Carg11845	Silver-seed gourd	carcgyB0222
Cucsa.232870	Carg10597	Silver-seed gourd	carcgyB0874

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.232870	Watermelon (97103) v2	cgywmbB408
Cucsa.232870	Watermelon (97103) v2	cgywmbB409
Cucsa.232870	Wax gourd	cgywgoB461
Cucsa.232870	Cucurbita maxima (Rimu)	cgycmaB0637
Cucsa.232870	Cucurbita maxima (Rimu)	cgycmaB0638
Cucsa.232870	Cucurbita maxima (Rimu)	cgycmaB0639
Cucsa.232870	Cucurbita maxima (Rimu)	cgycmaB0642
Cucsa.232870	Cucurbita moschata (Rifu)	cgycmoB0640
Cucsa.232870	Cucurbita moschata (Rifu)	cgycmoB0641
Cucsa.232870	Cucurbita moschata (Rifu)	cgycmoB0642
Cucsa.232870	Cucurbita moschata (Rifu)	cgycmoB0643
Cucsa.232870	Melon (DHL92) v3.5.1	cgymeB391
Cucsa.232870	Watermelon (Charleston Gray)	cgywcgB405
Cucsa.232870	Watermelon (Charleston Gray)	cgywcgB407
Cucsa.232870	Watermelon (97103) v1	cgywmB433
Cucsa.232870	Watermelon (97103) v1	cgywmB435
Cucsa.232870	Cucurbita pepo (Zucchini)	cgycpeB0611
Cucsa.232870	Cucurbita pepo (Zucchini)	cgycpeB0612
Cucsa.232870	Cucurbita pepo (Zucchini)	cgycpeB0613
Cucsa.232870	Cucurbita pepo (Zucchini)	cgycpeB0614
Cucsa.232870	Bottle gourd (USVL1VR-Ls)	cgylsiB378
Cucsa.232870	Melon (DHL92) v3.6.1	cgymedB389
Cucsa.232870	Cucumber (Chinese Long) v3	cgycucB349