CSPI02G19170 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI02G19170
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Calcium-binding EF-hand family protein, putative
Location	Chr2 : 17512682 .. 17513350 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACCAAGAGGCTACGCTTTTTAAGGACCCTTCCCTTCCACTTTCTTCTCAACAGTTCTTCAATCGAAGAAAAACGGCGAAGGTTGCGAAGTCGTCTAGGTTTCATCTAAAATGACTCGTCAATTATATGACAAGAAGAAATCAGGGTACAAGTTGCAAAAAGAAGAAATGAAAGAAATGTTTAGGGAGCATGATTTCAATGGCGACGGTCGCTTGAGCATTAAAGAATTGTCGAGAGCATTCGGCCAATTTGGCGCATTCTTCCCTCTCTACCGTGCAGCTTTTGGGTTGTTCCTTGCAGATGATGACGGCGATGGATTTATCAGTGAGCAAGAACTAGATAAAGTGGTAGATTATGCTATTAAATGTAAGTATAACCTACTTTAGTTATCTTTTCCATCCTGCTGTGGAAGGTTCCCTAGTGAGTGTGAGTTGTTGATGTAAAAAAATAATATACAGAAAGGTAATTTGTGTGTTCAAGAGATACCTTCCCCTATTATTGTAAAATGCCTTTCCTGATGGCTGATGCGAAGATGATGATGATAGCATTTCCAATAAAAATAAATTTTATGCTTCATTCTTGCTCTTGCATGATATGATAACAATTTTCTAAATATATGTATATTGTTACAAAATAATAGACGCACTTTATTTTAACAATGTACCAGGA

mRNA sequence

ATGACTCGTCAATTATATGACAAGAAGAAATCAGGGTACAAGTTGCAAAAAGAAGAAATGAAAGAAATGTTTAGGGAGCATGATTTCAATGGCGACGGTCGCTTGAGCATTAAAGAATTGTCGAGAGCATTCGGCCAATTTGGCGCATTCTTCCCTCTCTACCGTGCAGCTTTTGGGTTGTTCCTTGCAGATGATGACGGCGATGGATTTATCAGTGAGCAAGAACTAGATAAAGTGGTAGATTATGCTATTAAATGTAAGTATAACCTACTTTAG

Coding sequence (CDS)

ATGACTCGTCAATTATATGACAAGAAGAAATCAGGGTACAAGTTGCAAAAAGAAGAAATGAAAGAAATGTTTAGGGAGCATGATTTCAATGGCGACGGTCGCTTGAGCATTAAAGAATTGTCGAGAGCATTCGGCCAATTTGGCGCATTCTTCCCTCTCTACCGTGCAGCTTTTGGGTTGTTCCTTGCAGATGATGACGGCGATGGATTTATCAGTGAGCAAGAACTAGATAAAGTGGTAGATTATGCTATTAAATGTAAGTATAACCTACTTTAG

Homology

[Close]

BLAST of CSPI02G19170 vs. TrEMBL
Match: A0A0A0LKW4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 2.0e-44
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 1


Query: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60
          MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL
Sbjct: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60

Query: 61 FLADDDGDGFISEQELDKVVDYAIKCKYNLL 92
          FLADDDGDGFISEQELDKVVDYAIKCKYNLL
Sbjct: 61 FLADDDGDGFISEQELDKVVDYAIKCKYNLL 91

[Close]

BLAST of CSPI02G19170 vs. TrEMBL
Match: A0A0A0LRC1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 3.6e-17
Identity = 42/75 (56.00%), Postives = 61/75 (81.33%), Query Frame = 1


Query: 14 KLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGFISE 73
          +L +E++KE+FREHD +GDGRLS+ E+ +AFG FG+  P Y+A +G+  AD++GDGFI E
Sbjct: 9  QLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFIEE 68

Query: 74 QELDKVVDYAIKCKY 89
          +ELDK+VDYA K ++
Sbjct: 69 EELDKLVDYAQKFQH 83

[Close]

BLAST of CSPI02G19170 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.7e-14
Identity = 40/73 (54.79%), Postives = 52/73 (71.23%), Query Frame = 1


Query: 11 SGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGF 70
          S +   +EEMKE+FREHD +GDG LSI EL +A G  G   P Y+A +G+   D++GDG 
Sbjct: 7  SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66

Query: 71 ISEQELDKVVDYA 84
          ISE ELDK++DYA
Sbjct: 67 ISEDELDKLIDYA 79

[Close]

BLAST of CSPI02G19170 vs. TrEMBL
Match: A0A0A0KMQ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 4.1e-13
Identity = 39/82 (47.56%), Postives = 56/82 (68.29%), Query Frame = 1


Query: 4   QLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLA 63
           ++ D+K S   L +E +KE+F+ HD N DG L+  EL++AF   G+ FP Y+A +G+  A
Sbjct: 85  RVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLGSMFPFYKACYGMVYA 144

Query: 64  DDDGDGFISEQELDKVVDYAIK 86
           D + DG ISE ELDK++DYA K
Sbjct: 145 DANEDGLISEAELDKLIDYASK 166

[Close]

BLAST of CSPI02G19170 vs. TrEMBL
Match: M5XHL4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013918mg PE=4 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 4.5e-12
Identity = 40/83 (48.19%), Postives = 57/83 (68.67%), Query Frame = 1


Query: 9  KKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGD 68
          K++ +   KE+++ +F+ HD NGDG+LS  EL  AF + G+ +P  RA F    ADDDGD
Sbjct: 13 KRAKFPYTKEQIRNVFKRHDKNGDGQLSKDELDAAFKELGSIWPPGRAWFAQRYADDDGD 72

Query: 69 GFIS-EQELDKVVDYAIKCKYNL 91
          GFIS ++EL K+V YA++ KY L
Sbjct: 73 GFISIDKELSKLVQYALELKYTL 95

[Close]

BLAST of CSPI02G19170 vs. NCBI nr
Match: gi|700207396|gb|KGN62515.1| (hypothetical protein Csa_2G358860 [Cucumis sativus])

HSP 1 Score: 186.0 bits (471), Expect = 2.9e-44
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 1


Query: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60
          MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL
Sbjct: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60

Query: 61 FLADDDGDGFISEQELDKVVDYAIKCKYNLL 92
          FLADDDGDGFISEQELDKVVDYAIKCKYNLL
Sbjct: 61 FLADDDGDGFISEQELDKVVDYAIKCKYNLL 91

[Close]

BLAST of CSPI02G19170 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 171.8 bits (434), Expect = 5.6e-40
Identity = 85/85 (100.00%), Postives = 85/85 (100.00%), Query Frame = 1


Query: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60
          MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL
Sbjct: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60

Query: 61 FLADDDGDGFISEQELDKVVDYAIK 86
          FLADDDGDGFISEQELDKVVDYAIK
Sbjct: 61 FLADDDGDGFISEQELDKVVDYAIK 85

[Close]

BLAST of CSPI02G19170 vs. NCBI nr
Match: gi|700207397|gb|KGN62516.1| (hypothetical protein Csa_2G358870 [Cucumis sativus])

HSP 1 Score: 95.5 bits (236), Expect = 5.1e-17
Identity = 42/75 (56.00%), Postives = 61/75 (81.33%), Query Frame = 1


Query: 14 KLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGFISE 73
          +L +E++KE+FREHD +GDGRLS+ E+ +AFG FG+  P Y+A +G+  AD++GDGFI E
Sbjct: 9  QLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFIEE 68

Query: 74 QELDKVVDYAIKCKY 89
          +ELDK+VDYA K ++
Sbjct: 69 EELDKLVDYAQKFQH 83

[Close]

BLAST of CSPI02G19170 vs. NCBI nr
Match: gi|700207406|gb|KGN62525.1| (hypothetical protein Csa_2G359940 [Cucumis sativus])

HSP 1 Score: 86.7 bits (213), Expect = 2.4e-14
Identity = 40/73 (54.79%), Postives = 52/73 (71.23%), Query Frame = 1


Query: 11 SGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGF 70
          S +   +EEMKE+FREHD +GDG LSI EL +A G  G   P Y+A +G+   D++GDG 
Sbjct: 7  SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66

Query: 71 ISEQELDKVVDYA 84
          ISE ELDK++DYA
Sbjct: 67 ISEDELDKLIDYA 79

[Close]

BLAST of CSPI02G19170 vs. NCBI nr
Match: gi|700194510|gb|KGN49687.1| (hypothetical protein Csa_5G067410 [Cucumis sativus])

HSP 1 Score: 82.0 bits (201), Expect = 5.9e-13
Identity = 39/82 (47.56%), Postives = 56/82 (68.29%), Query Frame = 1


Query: 4   QLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLA 63
           ++ D+K S   L +E +KE+F+ HD N DG L+  EL++AF   G+ FP Y+A +G+  A
Sbjct: 85  RVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLGSMFPFYKACYGMVYA 144

Query: 64  DDDGDGFISEQELDKVVDYAIK 86
           D + DG ISE ELDK++DYA K
Sbjct: 145 DANEDGLISEAELDKLIDYASK 166

The following BLAST results are available for this feature:

BLAST of CSPI02G19170 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of CSPI02G19170 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LKW4_CUCSA	2.0e-44	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1	[more]
A0A0A0LRC1_CUCSA	3.6e-17	56.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1	[more]
A0A0A0LKX1_CUCSA	1.7e-14	54.79	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1	[more]
A0A0A0KMQ1_CUCSA	4.1e-13	47.56	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1	[more]
M5XHL4_PRUPE	4.5e-12	48.19	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013918mg PE=4 SV=1	[more]

BLAST of CSPI02G19170 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CSPI02G19170 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700207396\|gb\|KGN62515.1\|	2.9e-44	100.00	hypothetical protein Csa_2G358860 [Cucumis sativus]	[more]
gi\|449470198\|ref\|XP_004152805.1\|	5.6e-40	100.00	PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus]	[more]
gi\|700207397\|gb\|KGN62516.1\|	5.1e-17	56.00	hypothetical protein Csa_2G358870 [Cucumis sativus]	[more]
gi\|700207406\|gb\|KGN62525.1\|	2.4e-14	54.79	hypothetical protein Csa_2G359940 [Cucumis sativus]	[more]
gi\|700194510\|gb\|KGN49687.1\|	5.9e-13	47.56	hypothetical protein Csa_5G067410 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G19170.1	CSPI02G19170.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 18..75 score: 1.
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 53..83 score: 80.0coord: 19..47 score: 2.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 62..86 score: 7.428coord: 15..50 score: 14
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 10..80 score: 1.2
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 12..77 score: 1.36
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 28..40 score: -coord: 64..76 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 15..84 score: 1.8
None	No IPR available	PANTHER	PTHR10891:SF592	CALCIUM-BINDING PROTEIN CML38-RELATED	coord: 15..84 score: 1.8

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI02G19170	Cla022567	Watermelon (97103) v1	cpiwmB116
CSPI02G19170	Csa2G358860	Cucumber (Chinese Long) v2	cpicuB065
CSPI02G19170	MELO3C010868	Melon (DHL92) v3.5.1	cpimeB118
CSPI02G19170	ClCG08G015820	Watermelon (Charleston Gray)	cpiwcgB172
CSPI02G19170	MELO3C010869.2	Melon (DHL92) v3.6.1	cpimedB114
CSPI02G19170	CsaV3_2G027870	Cucumber (Chinese Long) v3	cpicucB081
CSPI02G19170	Bhi04G000312	Wax gourd	cpiwgoB159
CSPI02G19170	Cucsa.232870	Cucumber (Gy14) v1	cgycpiB341
CSPI02G19170	CmaCh12G005370	Cucurbita maxima (Rimu)	cmacpiB175
CSPI02G19170	CmoCh12G004810	Cucurbita moschata (Rifu)	cmocpiB160
CSPI02G19170	Cp4.1LG07g05370	Cucurbita pepo (Zucchini)	cpecpiB820

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI02G19170	Cucumber (Gy14) v1	cgycpiB331
CSPI02G19170	Cucurbita maxima (Rimu)	cmacpiB011
CSPI02G19170	Cucurbita maxima (Rimu)	cmacpiB439
CSPI02G19170	Cucurbita maxima (Rimu)	cmacpiB513
CSPI02G19170	Cucurbita maxima (Rimu)	cmacpiB793
CSPI02G19170	Cucurbita moschata (Rifu)	cmocpiB002
CSPI02G19170	Cucurbita moschata (Rifu)	cmocpiB428
CSPI02G19170	Cucurbita moschata (Rifu)	cmocpiB782
CSPI02G19170	Cucumber (Chinese Long) v2	cpicuB080
CSPI02G19170	Cucumber (Chinese Long) v2	cpicuB096
CSPI02G19170	Melon (DHL92) v3.5.1	cpimeB105
CSPI02G19170	Melon (DHL92) v3.5.1	cpimeB148
CSPI02G19170	Watermelon (Charleston Gray)	cpiwcgB136
CSPI02G19170	Watermelon (Charleston Gray)	cpiwcgB144
CSPI02G19170	Watermelon (97103) v1	cpiwmB129
CSPI02G19170	Watermelon (97103) v1	cpiwmB153
CSPI02G19170	Cucurbita pepo (Zucchini)	cpecpiB092
CSPI02G19170	Cucurbita pepo (Zucchini)	cpecpiB541
CSPI02G19170	Cucurbita pepo (Zucchini)	cpecpiB773
CSPI02G19170	Bottle gourd (USVL1VR-Ls)	cpilsiB114
CSPI02G19170	Bottle gourd (USVL1VR-Ls)	cpilsiB141
CSPI02G19170	Melon (DHL92) v3.6.1	cpimedB100
CSPI02G19170	Melon (DHL92) v3.6.1	cpimedB142
CSPI02G19170	Cucumber (Gy14) v2	cgybcpiB057
CSPI02G19170	Cucumber (Gy14) v2	cgybcpiB163
CSPI02G19170	Cucumber (Gy14) v2	cgybcpiB305
CSPI02G19170	Silver-seed gourd	carcpiB0209
CSPI02G19170	Silver-seed gourd	carcpiB0806
CSPI02G19170	Cucumber (Chinese Long) v3	cpicucB103
CSPI02G19170	Watermelon (97103) v2	cpiwmbB137
CSPI02G19170	Watermelon (97103) v2	cpiwmbB166
CSPI02G19170	Wax gourd	cpiwgoB198
CSPI02G19170	Wild cucumber (PI 183967)	cpicpiB074
CSPI02G19170	Wild cucumber (PI 183967)	cpicpiB091