ClCG08G015820 (gene) Watermelon (Charleston Gray) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	ClCG08G015820
Type	gene
Organism	Citrullus lanatus (Watermelon (Charleston Gray))
Description	Calcium-binding EF-hand family protein LENGTH=161
Location	CG_Chr08 : 28265326 .. 28265544 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGAAATCTTCCGAGAGCACGACATCGACGGAGACGGTTACCTCAGCATCAGTGAGCTCATCAAAGCCATGGGGTTTTTGGGTCACTCTATACCATTCTACAAAGCTCATTACGGTATGGCTTATTCTGATGAGAATGGTGACGGCTTCATCTCTGAAGATGAGCTTGACAAACTCATTGATTATGTCGAAAAGTTTCAGTACAATAAGCGTTAA

mRNA sequence

ATGAAGGAAATCTTCCGAGAGCACGACATCGACGGAGACGGTTACCTCAGCATCAGTGAGCTCATCAAAGCCATGGGGTTTTTGGGTCACTCTATACCATTCTACAAAGCTCATTACGGTATGGCTTATTCTGATGAGAATGGTGACGGCTTCATCTCTGAAGATGAGCTTGACAAACTCATTGATTATGTCGAAAAGTTTCAGTACAATAAGCGTTAA

Coding sequence (CDS)

ATGAAGGAAATCTTCCGAGAGCACGACATCGACGGAGACGGTTACCTCAGCATCAGTGAGCTCATCAAAGCCATGGGGTTTTTGGGTCACTCTATACCATTCTACAAAGCTCATTACGGTATGGCTTATTCTGATGAGAATGGTGACGGCTTCATCTCTGAAGATGAGCTTGACAAACTCATTGATTATGTCGAAAAGTTTCAGTACAATAAGCGTTAA

Protein sequence

MKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYSDENGDGFISEDELDKLIDYVEKFQYNKR

Homology

[Close]

BLAST of ClCG08G015820 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 2.9e-30
Identity = 65/72 (90.28%), Postives = 67/72 (93.06%), Query Frame = 1


Query: 1  MKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYSDENGDGFISEDELDKL 60
          MKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAY DENGDG ISEDELDKL
Sbjct: 16 MKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGLISEDELDKL 75

Query: 61 IDYVEKFQYNKR 73
          IDY E+FQ  +R
Sbjct: 76 IDYAERFQKRRR 87

[Close]

BLAST of ClCG08G015820 vs. TrEMBL
Match: A0A0A0LRC1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 3.4e-23
Identity = 49/72 (68.06%), Postives = 63/72 (87.50%), Query Frame = 1


Query: 1  MKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYSDENGDGFISEDELDKL 60
          +KEIFREHDIDGDG LS++E++KA G  G  +PFYKAHYGMA++DENGDGFI E+ELDKL
Sbjct: 15 IKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFIEEEELDKL 74

Query: 61 IDYVEKFQYNKR 73
          +DY +KFQ+ ++
Sbjct: 75 VDYAQKFQHKRK 86

[Close]

BLAST of ClCG08G015820 vs. TrEMBL
Match: A0A0A0KIY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 1.5e-15
Identity = 41/72 (56.94%), Postives = 55/72 (76.39%), Query Frame = 1


Query: 1   MKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYSDENGDGFISEDELDKL 60
           +KEIF+ HD D DG+L++ E+ KA  FLG  +P YKA++GM Y+D N DG IS+DEL+KL
Sbjct: 106 IKEIFQYHDSDNDGFLNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKL 165

Query: 61  IDYVEKFQYNKR 73
           +DY  KF +NKR
Sbjct: 166 VDYAYKF-FNKR 176

[Close]

BLAST of ClCG08G015820 vs. TrEMBL
Match: A0A0A0LKW4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 4.5e-15
Identity = 40/70 (57.14%), Postives = 52/70 (74.29%), Query Frame = 1


Query: 1  MKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYSDENGDGFISEDELDKL 60
          MKE+FREHD +GDG LSI EL +A G  G   P Y+A +G+  +D++GDGFISE ELDK+
Sbjct: 20 MKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGFISEQELDKV 79

Query: 61 IDYVEKFQYN 71
          +DY  K +YN
Sbjct: 80 VDYAIKCKYN 89

[Close]

BLAST of ClCG08G015820 vs. TrEMBL
Match: A0A0A0KMQ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 3.8e-14
Identity = 41/66 (62.12%), Postives = 47/66 (71.21%), Query Frame = 1


Query: 1   MKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYSDENGDGFISEDELDKL 60
           +KEIF+ HD + DG+L+  EL KA  FLG   PFYKA YGM Y+D N DG ISE ELDKL
Sbjct: 101 IKEIFQYHDNNNDGFLNRMELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKL 160

Query: 61  IDYVEK 67
           IDY  K
Sbjct: 161 IDYASK 166

[Close]

BLAST of ClCG08G015820 vs. NCBI nr
Match: gi|700207406|gb|KGN62525.1| (hypothetical protein Csa_2G359940 [Cucumis sativus])

HSP 1 Score: 138.7 bits (348), Expect = 4.1e-30
Identity = 65/72 (90.28%), Postives = 67/72 (93.06%), Query Frame = 1


Query: 1  MKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYSDENGDGFISEDELDKL 60
          MKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAY DENGDG ISEDELDKL
Sbjct: 16 MKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGLISEDELDKL 75

Query: 61 IDYVEKFQYNKR 73
          IDY E+FQ  +R
Sbjct: 76 IDYAERFQKRRR 87

[Close]

BLAST of ClCG08G015820 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 115.2 bits (287), Expect = 4.9e-23
Identity = 49/72 (68.06%), Postives = 63/72 (87.50%), Query Frame = 1


Query: 1   MKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYSDENGDGFISEDELDKL 60
           +KEIFREHDIDGDG LS++E++KA G  G  +PFYKAHYGMA++DENGDGFI E+ELDKL
Sbjct: 96  IKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFIEEEELDKL 155

Query: 61  IDYVEKFQYNKR 73
           +DY +KFQ+ ++
Sbjct: 156 VDYAQKFQHKRK 167

[Close]

BLAST of ClCG08G015820 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 83.6 bits (205), Expect = 1.6e-13
Identity = 38/66 (57.58%), Postives = 49/66 (74.24%), Query Frame = 1


Query: 1  MKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYSDENGDGFISEDELDKL 60
          MKE+FREHD +GDG LSI EL +A G  G   P Y+A +G+  +D++GDGFISE ELDK+
Sbjct: 20 MKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGFISEQELDKV 79

Query: 61 IDYVEK 67
          +DY  K
Sbjct: 80 VDYAIK 85

HSP 2 Score: 115.2 bits (287), Expect = 4.9e-23
Identity = 49/72 (68.06%), Postives = 63/72 (87.50%), Query Frame = 1


Query: 1  MKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYSDENGDGFISEDELDKL 60
          +KEIFREHDIDGDG LS++E++KA G  G  +PFYKAHYGMA++DENGDGFI E+ELDKL
Sbjct: 15 IKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFIEEEELDKL 74

Query: 61 IDYVEKFQYNKR 73
          +DY +KFQ+ ++
Sbjct: 75 VDYAQKFQHKRK 86

[Close]

BLAST of ClCG08G015820 vs. NCBI nr
Match: gi|700194513|gb|KGN49690.1| (hypothetical protein Csa_5G068170 [Cucumis sativus])

HSP 1 Score: 89.7 bits (221), Expect = 2.2e-15
Identity = 41/72 (56.94%), Postives = 55/72 (76.39%), Query Frame = 1


Query: 1   MKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYSDENGDGFISEDELDKL 60
           +KEIF+ HD D DG+L++ E+ KA  FLG  +P YKA++GM Y+D N DG IS+DEL+KL
Sbjct: 106 IKEIFQYHDSDNDGFLNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKL 165

Query: 61  IDYVEKFQYNKR 73
           +DY  KF +NKR
Sbjct: 166 VDYAYKF-FNKR 176

[Close]

BLAST of ClCG08G015820 vs. NCBI nr
Match: gi|700207396|gb|KGN62515.1| (hypothetical protein Csa_2G358860 [Cucumis sativus])

HSP 1 Score: 88.2 bits (217), Expect = 6.4e-15
Identity = 40/70 (57.14%), Postives = 52/70 (74.29%), Query Frame = 1


Query: 1  MKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYSDENGDGFISEDELDKL 60
          MKE+FREHD +GDG LSI EL +A G  G   P Y+A +G+  +D++GDGFISE ELDK+
Sbjct: 20 MKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGFISEQELDKV 79

Query: 61 IDYVEKFQYN 71
          +DY  K +YN
Sbjct: 80 VDYAIKCKYN 89

The following BLAST results are available for this feature:

BLAST of ClCG08G015820 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LKX1_CUCSA	2.9e-30	90.28	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1	[more]
A0A0A0LRC1_CUCSA	3.4e-23	68.06	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1	[more]
A0A0A0KIY4_CUCSA	1.5e-15	56.94	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1	[more]
A0A0A0LKW4_CUCSA	4.5e-15	57.14	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1	[more]
A0A0A0KMQ1_CUCSA	3.8e-14	62.12	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1	[more]

BLAST of ClCG08G015820 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700207406\|gb\|KGN62525.1\|	4.1e-30	90.28	hypothetical protein Csa_2G359940 [Cucumis sativus]	[more]
gi\|449470198\|ref\|XP_004152805.1\|	4.9e-23	68.06	PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus]	[more]
gi\|449470198\|ref\|XP_004152805.1\|	1.6e-13	57.58	PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus]	[more]
gi\|700194513\|gb\|KGN49690.1\|	2.2e-15	56.94	hypothetical protein Csa_5G068170 [Cucumis sativus]	[more]
gi\|700207396\|gb\|KGN62515.1\|	6.4e-15	57.14	hypothetical protein Csa_2G358860 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG08G015820.1	ClCG08G015820.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 2..25 score: 1.
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 45..60 score: 0
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 1..31 score: 14.374coord: 45..67 score: 8
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 2..62 score: 1.3
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 1..60 score: 2.88
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 9..21 score: -coord: 45..57 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 1..63 score: 2.1
None	No IPR available	PANTHER	PTHR10891:SF592	CALCIUM-BINDING PROTEIN CML38-RELATED	coord: 1..63 score: 2.1

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
ClCG08G015820	Cla022563	Watermelon (97103) v1	wcgwmB400
ClCG08G015820	Cla97C08G159390	Watermelon (97103) v2	wcgwmbB336
ClCG08G015820	Bhi04G000306	Wax gourd	wcgwgoB612
ClCG08G015820	CSPI02G19170	Wild cucumber (PI 183967)	cpiwcgB172
ClCG08G015820	CmaCh12G005390	Cucurbita maxima (Rimu)	cmawcgB169
ClCG08G015820	CmoCh12G004830	Cucurbita moschata (Rifu)	cmowcgB161
ClCG08G015820	MELO3C010868.2	Melon (DHL92) v3.6.1	medwcgB288
ClCG08G015820	CsaV3_2G027870	Cucumber (Chinese Long) v3	cucwcgB176

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
ClCG08G015820	Silver-seed gourd	carwcgB0178
ClCG08G015820	Silver-seed gourd	carwcgB0752
ClCG08G015820	Cucumber (Chinese Long) v3	cucwcgB350
ClCG08G015820	Watermelon (97103) v2	wcgwmbB327
ClCG08G015820	Wax gourd	wcgwgoB656
ClCG08G015820	Watermelon (Charleston Gray)	wcgwcgB138
ClCG08G015820	Watermelon (Charleston Gray)	wcgwcgB166
ClCG08G015820	Cucumber (Gy14) v1	cgywcgB195
ClCG08G015820	Cucumber (Gy14) v1	cgywcgB399
ClCG08G015820	Cucumber (Gy14) v1	cgywcgB407
ClCG08G015820	Cucurbita maxima (Rimu)	cmawcgB029
ClCG08G015820	Cucurbita maxima (Rimu)	cmawcgB140
ClCG08G015820	Cucurbita maxima (Rimu)	cmawcgB434
ClCG08G015820	Cucurbita maxima (Rimu)	cmawcgB712
ClCG08G015820	Cucurbita moschata (Rifu)	cmowcgB023
ClCG08G015820	Cucurbita moschata (Rifu)	cmowcgB123
ClCG08G015820	Cucurbita moschata (Rifu)	cmowcgB435
ClCG08G015820	Cucurbita moschata (Rifu)	cmowcgB716
ClCG08G015820	Wild cucumber (PI 183967)	cpiwcgB350
ClCG08G015820	Wild cucumber (PI 183967)	cpiwcgB601
ClCG08G015820	Cucumber (Chinese Long) v2	cuwcgB169
ClCG08G015820	Cucumber (Chinese Long) v2	cuwcgB332
ClCG08G015820	Cucumber (Chinese Long) v2	cuwcgB568
ClCG08G015820	Melon (DHL92) v3.5.1	mewcgB295
ClCG08G015820	Melon (DHL92) v3.5.1	mewcgB223
ClCG08G015820	Melon (DHL92) v3.5.1	mewcgB502
ClCG08G015820	Watermelon (97103) v1	wcgwmB412
ClCG08G015820	Watermelon (97103) v1	wcgwmB421
ClCG08G015820	Cucurbita pepo (Zucchini)	cpewcgB125
ClCG08G015820	Cucurbita pepo (Zucchini)	cpewcgB528
ClCG08G015820	Cucurbita pepo (Zucchini)	cpewcgB624
ClCG08G015820	Cucurbita pepo (Zucchini)	cpewcgB708
ClCG08G015820	Cucurbita pepo (Zucchini)	cpewcgB748
ClCG08G015820	Bottle gourd (USVL1VR-Ls)	lsiwcgB305
ClCG08G015820	Bottle gourd (USVL1VR-Ls)	lsiwcgB438
ClCG08G015820	Cucumber (Gy14) v2	cgybwcgB155
ClCG08G015820	Cucumber (Gy14) v2	cgybwcgB309
ClCG08G015820	Cucumber (Gy14) v2	cgybwcgB529
ClCG08G015820	Melon (DHL92) v3.6.1	medwcgB214
ClCG08G015820	Melon (DHL92) v3.6.1	medwcgB496