Cp4.1LG07g05370 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG07g05370
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionCalcium-binding EF-hand family protein, putative
LocationCp4.1LG07 : 3101766 .. 3102038 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAGTAAATATGGCAAGAACAACTCAAGCTCCATGTTGCCAAGAGAGGAGATAAAAGCAATATTTAAGGAACATGATTTCAATGGCGATGGCCGATTGAGCATCCAAGAATTGTCGAGAGCGTTCGGAATGCTGGGCGCATTTTTACCTCTCTTCCGTGCAGTCCGTGGCATGCTCATTGCTGATGTCAATGGAGATGGATATATCACCGAGCATGAACTGGATAAGGTCGTGGATTATGCTATAAAATGTACGTATAACATAATCTAA

mRNA sequence

ATGGCTAGTAAATATGGCAAGAACAACTCAAGCTCCATGTTGCCAAGAGAGGAGATAAAAGCAATATTTAAGGAACATGATTTCAATGGCGATGGCCGATTGAGCATCCAAGAATTGTCGAGAGCGTTCGGAATGCTGGGCGCATTTTTACCTCTCTTCCGTGCAGTCCGTGGCATGCTCATTGCTGATGTCAATGGAGATGGATATATCACCGAGCATGAACTGGATAAGGTCGTGGATTATGCTATAAAATGTACGTATAACATAATCTAA

Coding sequence (CDS)

ATGGCTAGTAAATATGGCAAGAACAACTCAAGCTCCATGTTGCCAAGAGAGGAGATAAAAGCAATATTTAAGGAACATGATTTCAATGGCGATGGCCGATTGAGCATCCAAGAATTGTCGAGAGCGTTCGGAATGCTGGGCGCATTTTTACCTCTCTTCCGTGCAGTCCGTGGCATGCTCATTGCTGATGTCAATGGAGATGGATATATCACCGAGCATGAACTGGATAAGGTCGTGGATTATGCTATAAAATGTACGTATAACATAATCTAA

Protein sequence

MASKYGKNNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADVNGDGYITEHELDKVVDYAIKCTYNII
Homology
[Close]
BLAST of Cp4.1LG07g05370 vs. TrEMBL
Match: A0A0A0LKW4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 1.6e-25
Identity = 56/86 (65.12%), Postives = 69/86 (80.23%), Query Frame = 1 

Query: 5  YGKNNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADV 64
          Y K  S   L +EE+K +F+EHDFNGDGRLSI+ELSRAFG  GAF PL+RA  G+ +AD 
Sbjct: 6  YDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADD 65

Query: 65 NGDGYITEHELDKVVDYAIKCTYNII 91
          +GDG+I+E ELDKVVDYAIKC YN++
Sbjct: 66 DGDGFISEQELDKVVDYAIKCKYNLL 91

[Close]
BLAST of Cp4.1LG07g05370 vs. TrEMBL
Match: A0A0A0LRC1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 2.3e-16
Identity = 43/71 (60.56%), Postives = 54/71 (76.06%), Query Frame = 1 

Query: 14 LPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADVNGDGYITEH 73
          L RE+IK IF+EHD +GDGRLS+ E+ +AFG  G+ LP ++A  GM  AD NGDG+I E 
Sbjct: 10 LSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFIEEE 69

Query: 74 ELDKVVDYAIK 85
          ELDK+VDYA K
Sbjct: 70 ELDKLVDYAQK 80

[Close]
BLAST of Cp4.1LG07g05370 vs. TrEMBL
Match: A0A0D2QZC2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_010G019300 PE=4 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 2.8e-14
Identity = 40/72 (55.56%), Postives = 54/72 (75.00%), Query Frame = 1 

Query: 18  EIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADVNGDGYITEHELDK 77
           ++KA FK  D N DGRLSI+EL +AF  LGA++P +RA RG+ +AD NGDGY+++ ELD 
Sbjct: 30  QLKAAFKSFDVNKDGRLSIEELRKAFASLGAYIPGWRARRGLSVADGNGDGYVSDDELDD 89

Query: 78  VVDYAIKCTYNI 90
           +V YA+K  Y I
Sbjct: 90  LVKYAMKQGYTI 101

[Close]
BLAST of Cp4.1LG07g05370 vs. TrEMBL
Match: A0A061FBR8_THECC (Calcium-binding EF-hand family protein, putative OS=Theobroma cacao GN=TCM_033854 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 8.0e-14
Identity = 40/84 (47.62%), Postives = 58/84 (69.05%), Query Frame = 1 

Query: 4  KYGKNNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIAD 63
          K  +  +  +L   E+++IFK  D+N DGRLS QEL +AF  LG+ LP +RA RG+  AD
Sbjct: 14 KCRRKTTPMLLTGTELRSIFKSFDYNQDGRLSKQELRKAFASLGSHLPGWRAGRGLHHAD 73

Query: 64 VNGDGYITEHELDKVVDYAIKCTY 88
           N DGY+++ ELD +V+YA++C Y
Sbjct: 74 ANRDGYVSDDELDDLVEYALECGY 97

[Close]
BLAST of Cp4.1LG07g05370 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 8.0e-14
Identity = 39/67 (58.21%), Postives = 49/67 (73.13%), Query Frame = 1 

Query: 16 REEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADVNGDGYITEHEL 75
          REE+K IF+EHD +GDG LSI EL +A G LG  +P ++A  GM   D NGDG I+E EL
Sbjct: 13 REEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGLISEDEL 72

Query: 76 DKVVDYA 83
          DK++DYA
Sbjct: 73 DKLIDYA 79

[Close]
BLAST of Cp4.1LG07g05370 vs. TAIR10
Match: AT1G76650.1 (AT1G76650.1 calmodulin-like 38)

HSP 1 Score: 47.4 bits (111), Expect = 5.5e-06
Identity = 24/72 (33.33%), Postives = 41/72 (56.94%), Query Frame = 1 

Query: 8   NNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADVNGD 67
           N+S+      E++A+F   D N DGR+S +EL ++F  LG  L    AV  + ++D +GD
Sbjct: 33  NSSNGEDKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGD 92

Query: 68  GYITEHELDKVV 80
           G +   E  +++
Sbjct: 93  GMLDFEEFSQLI 104

[Close]
BLAST of Cp4.1LG07g05370 vs. NCBI nr
Match: gi|700207396|gb|KGN62515.1| (hypothetical protein Csa_2G358860 [Cucumis sativus])

HSP 1 Score: 123.2 bits (308), Expect = 2.2e-25
Identity = 56/86 (65.12%), Postives = 69/86 (80.23%), Query Frame = 1 

Query: 5  YGKNNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADV 64
          Y K  S   L +EE+K +F+EHDFNGDGRLSI+ELSRAFG  GAF PL+RA  G+ +AD 
Sbjct: 6  YDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADD 65

Query: 65 NGDGYITEHELDKVVDYAIKCTYNII 91
          +GDG+I+E ELDKVVDYAIKC YN++
Sbjct: 66 DGDGFISEQELDKVVDYAIKCKYNLL 91

[Close]
BLAST of Cp4.1LG07g05370 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 115.5 bits (288), Expect = 4.7e-23
Identity = 54/82 (65.85%), Postives = 65/82 (79.27%), Query Frame = 1 

Query: 5  YGKNNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADV 64
          Y K  S   L +EE+K +F+EHDFNGDGRLSI+ELSRAFG  GAF PL+RA  G+ +AD 
Sbjct: 6  YDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADD 65

Query: 65 NGDGYITEHELDKVVDYAIKCT 87
          +GDG+I+E ELDKVVDYAIK T
Sbjct: 66 DGDGFISEQELDKVVDYAIKST 87

[Close]
BLAST of Cp4.1LG07g05370 vs. NCBI nr
Match: gi|700207397|gb|KGN62516.1| (hypothetical protein Csa_2G358870 [Cucumis sativus])

HSP 1 Score: 92.8 bits (229), Expect = 3.2e-16
Identity = 43/71 (60.56%), Postives = 54/71 (76.06%), Query Frame = 1 

Query: 14 LPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADVNGDGYITEH 73
          L RE+IK IF+EHD +GDGRLS+ E+ +AFG  G+ LP ++A  GM  AD NGDG+I E 
Sbjct: 10 LSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFIEEE 69

Query: 74 ELDKVVDYAIK 85
          ELDK+VDYA K
Sbjct: 70 ELDKLVDYAQK 80

[Close]
BLAST of Cp4.1LG07g05370 vs. NCBI nr
Match: gi|823232722|ref|XP_012449049.1| (PREDICTED: polcalcin Che a 3-like [Gossypium raimondii])

HSP 1 Score: 85.9 bits (211), Expect = 4.0e-14
Identity = 40/72 (55.56%), Postives = 54/72 (75.00%), Query Frame = 1 

Query: 18  EIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIADVNGDGYITEHELDK 77
           ++KA FK  D N DGRLSI+EL +AF  LGA++P +RA RG+ +AD NGDGY+++ ELD 
Sbjct: 30  QLKAAFKSFDVNKDGRLSIEELRKAFASLGAYIPGWRARRGLSVADGNGDGYVSDDELDD 89

Query: 78  VVDYAIKCTYNI 90
           +V YA+K  Y I
Sbjct: 90  LVKYAMKQGYTI 101

[Close]
BLAST of Cp4.1LG07g05370 vs. NCBI nr
Match: gi|590592372|ref|XP_007017261.1| (Calcium-binding EF-hand family protein, putative [Theobroma cacao])

HSP 1 Score: 84.3 bits (207), Expect = 1.2e-13
Identity = 40/84 (47.62%), Postives = 58/84 (69.05%), Query Frame = 1 

Query: 4  KYGKNNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLFRAVRGMLIAD 63
          K  +  +  +L   E+++IFK  D+N DGRLS QEL +AF  LG+ LP +RA RG+  AD
Sbjct: 14 KCRRKTTPMLLTGTELRSIFKSFDYNQDGRLSKQELRKAFASLGSHLPGWRAGRGLHHAD 73

Query: 64 VNGDGYITEHELDKVVDYAIKCTY 88
           N DGY+++ ELD +V+YA++C Y
Sbjct: 74 ANRDGYVSDDELDDLVEYALECGY 97

The following BLAST results are available for this feature:
BLAST of Cp4.1LG07g05370 vs. Swiss-Prot
Match NameE-valueIdentityDescription
BLAST of Cp4.1LG07g05370 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LKW4_CUCSA1.6e-2565.12Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358860 PE=4 SV=1[more]
A0A0A0LRC1_CUCSA2.3e-1660.56Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1[more]
A0A0D2QZC2_GOSRA2.8e-1455.56Uncharacterized protein OS=Gossypium raimondii GN=B456_010G019300 PE=4 SV=1[more]
A0A061FBR8_THECC8.0e-1447.62Calcium-binding EF-hand family protein, putative OS=Theobroma cacao GN=TCM_03385... [more]
A0A0A0LKX1_CUCSA8.0e-1458.21Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1[more]
BLAST of Cp4.1LG07g05370 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G76650.15.5e-0633.33 calmodulin-like 38[more]
BLAST of Cp4.1LG07g05370 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700207396|gb|KGN62515.1|2.2e-2565.12hypothetical protein Csa_2G358860 [Cucumis sativus][more]
gi|449470198|ref|XP_004152805.1|4.7e-2365.85PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus][more]
gi|700207397|gb|KGN62516.1|3.2e-1660.56hypothetical protein Csa_2G358870 [Cucumis sativus][more]
gi|823232722|ref|XP_012449049.1|4.0e-1455.56PREDICTED: polcalcin Che a 3-like [Gossypium raimondii][more]
gi|590592372|ref|XP_007017261.1|1.2e-1347.62Calcium-binding EF-hand family protein, putative [Theobroma cacao][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR018247EF_Hand_1_Ca_BS
IPR011992EF-hand-dom_pair
IPR002048EF_hand_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG07g05370.1Cp4.1LG07g05370.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 62..79
score: 0.
IPR002048EF-hand domainPFAMPF13405EF-hand_6coord: 18..47
score: 1.
IPR002048EF-hand domainSMARTSM00054efh_1coord: 18..46
score: 1.5E-4coord: 54..82
score:
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 14..49
score: 14.151coord: 63..85
score: 7
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 16..78
score: 1.1
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 11..76
score: 2.57
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 27..39
score: -coord: 63..75
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 8..83
score: 9.1
NoneNo IPR availablePANTHERPTHR10891:SF592CALCIUM-BINDING PROTEIN CML38-RELATEDcoord: 8..83
score: 9.1

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG07g05370Cla022567Watermelon (97103) v1cpewmB782
Cp4.1LG07g05370Csa2G358860Cucumber (Chinese Long) v2cpecuB819
Cp4.1LG07g05370MELO3C010868Melon (DHL92) v3.5.1cpemeB754
Cp4.1LG07g05370ClCG08G015860Watermelon (Charleston Gray)cpewcgB748
Cp4.1LG07g05370CSPI02G19170Wild cucumber (PI 183967)cpecpiB820
Cp4.1LG07g05370Lsi08G014660Bottle gourd (USVL1VR-Ls)cpelsiB685
Cp4.1LG07g05370CsaV3_4G034260Cucumber (Chinese Long) v3cpecucB1025
Cp4.1LG07g05370CsaV3_2G027870Cucumber (Chinese Long) v3cpecucB1013
Cp4.1LG07g05370CsGy4G022320Cucumber (Gy14) v2cgybcpeB572
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG07g05370Cucurbita pepo (Zucchini)cpecpeB146
Cp4.1LG07g05370Cucurbita pepo (Zucchini)cpecpeB471
Cp4.1LG07g05370Cucurbita pepo (Zucchini)cpecpeB500
Cp4.1LG07g05370Cucurbita pepo (Zucchini)cpecpeB510
Cp4.1LG07g05370Cucumber (Gy14) v1cgycpeB0614
Cp4.1LG07g05370Cucurbita maxima (Rimu)cmacpeB065
Cp4.1LG07g05370Cucurbita maxima (Rimu)cmacpeB203
Cp4.1LG07g05370Cucurbita maxima (Rimu)cmacpeB516
Cp4.1LG07g05370Cucurbita maxima (Rimu)cmacpeB797
Cp4.1LG07g05370Cucurbita moschata (Rifu)cmocpeB037
Cp4.1LG07g05370Cucurbita moschata (Rifu)cmocpeB174
Cp4.1LG07g05370Cucurbita moschata (Rifu)cmocpeB474
Cp4.1LG07g05370Cucurbita moschata (Rifu)cmocpeB750
Cp4.1LG07g05370Wild cucumber (PI 183967)cpecpiB833
Cp4.1LG07g05370Cucumber (Chinese Long) v2cpecuB832
Cp4.1LG07g05370Bottle gourd (USVL1VR-Ls)cpelsiB676
Cp4.1LG07g05370Watermelon (Charleston Gray)cpewcgB738
Cp4.1LG07g05370Watermelon (97103) v1cpewmB795
Cp4.1LG07g05370Melon (DHL92) v3.5.1cpemeB784
Cp4.1LG07g05370Cucumber (Gy14) v2cgybcpeB270
Cp4.1LG07g05370Cucumber (Gy14) v2cgybcpeB988
Cp4.1LG07g05370Melon (DHL92) v3.6.1cpemedB883
Cp4.1LG07g05370Melon (DHL92) v3.6.1cpemedB890
Cp4.1LG07g05370Melon (DHL92) v3.6.1cpemedB921
Cp4.1LG07g05370Silver-seed gourdcarcpeB0113
Cp4.1LG07g05370Silver-seed gourdcarcpeB0268
Cp4.1LG07g05370Silver-seed gourdcarcpeB1084
Cp4.1LG07g05370Wax gourdcpewgoB1028
Cp4.1LG07g05370Wax gourdcpewgoB1067