CSPI06G19980 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI06G19980
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionCalcium-binding EF-hand family protein
LocationChr6 : 17929297 .. 17930074 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCACACACATATTATATATTTTGGTAATAAACTTTCTCGTCTGTGTTTACATTTCAATTCAAAACCAAAGAAACAAAAACACCATGCCTCCAATTAGCTCCAAAGACTTGCACAGGATCTTCAAGAAGCTAGACAAGAATTGTGATGGCCTCATTTGCCTTCAAGAGCTCAACTGGCTCTTGGATAGCATTGGCATCCAATTAACCATGGAGGAGTTGGAATCATTTCTAGAGAGACCAAGCCTCGACTTCGACGAGTTCTTGTTCTTTTACGAGTCAATATCCAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGTGGCAGGGTGTGTGCAAGATAATGATAATGATCAGGATCAGGATGATATGGAGATTGTATATTTGGCTTTTAAAGTGTTTGACATGAATGGTGATGGCTTCATTTCTTGCGACGAGCTCGAAAACGTGTTGGTGAAGCTCGAGCTTTGGGATGCAAGTAGAAGCGATGTAGATTATTGTAGAAGCATGATCCGAGCTTATGACACCAATTTGGATGGAAAGCTGGATTTTGAGGAGTTTAAGAACATGATGTTGCTCACAACATAGTTCTTCTTTTAGAGTTTCAGAAAAGACTAGAAAAAACATATCCCCTTTGTTATATTATTGTTAGATAGAGATGGAGTTTGGTCCGTTTATTTACATGTACAATTTGAGAAGCTGGGAAGCTTCAAGAATAAAAATTTTGGTCATGGAGTTCATGTAATTATATTTTTGGTGCTCCAATAAAATAATGCATTTCCA

mRNA sequence

ATGCCTCCAATTAGCTCCAAAGACTTGCACAGGATCTTCAAGAAGCTAGACAAGAATTGTGATGGCCTCATTTGCCTTCAAGAGCTCAACTGGCTCTTGGATAGCATTGGCATCCAATTAACCATGGAGGAGTTGGAATCATTTCTAGAGAGACCAAGCCTCGACTTCGACGAGTTCTTGTTCTTTTACGAGTCAATATCCAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGTGGCAGGGTGTGTGCAAGATAATGATAATGATCAGGATCAGGATGATATGGAGATTGTATATTTGGCTTTTAAAGTGTTTGACATGAATGGTGATGGCTTCATTTCTTGCGACGAGCTCGAAAACGTGTTGGTGAAGCTCGAGCTTTGGGATGCAAGTAGAAGCGATGTAGATTATTGTAGAAGCATGATCCGAGCTTATGACACCAATTTGGATGGAAAGCTGGATTTTGAGGAGTTTAAGAACATGATGTTGCTCACAACATAG

Coding sequence (CDS)

ATGCCTCCAATTAGCTCCAAAGACTTGCACAGGATCTTCAAGAAGCTAGACAAGAATTGTGATGGCCTCATTTGCCTTCAAGAGCTCAACTGGCTCTTGGATAGCATTGGCATCCAATTAACCATGGAGGAGTTGGAATCATTTCTAGAGAGACCAAGCCTCGACTTCGACGAGTTCTTGTTCTTTTACGAGTCAATATCCAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGTGGCAGGGTGTGTGCAAGATAATGATAATGATCAGGATCAGGATGATATGGAGATTGTATATTTGGCTTTTAAAGTGTTTGACATGAATGGTGATGGCTTCATTTCTTGCGACGAGCTCGAAAACGTGTTGGTGAAGCTCGAGCTTTGGGATGCAAGTAGAAGCGATGTAGATTATTGTAGAAGCATGATCCGAGCTTATGACACCAATTTGGATGGAAAGCTGGATTTTGAGGAGTTTAAGAACATGATGTTGCTCACAACATAG
Homology
[Close]
BLAST of CSPI06G19980 vs. Swiss-Prot
Match: CML44_ARATH (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana GN=CML44 PE=2 SV=2)

HSP 1 Score: 127.9 bits (320), Expect = 1.1e-28
Identity = 77/166 (46.39%), Postives = 98/166 (59.04%), Query Frame = 1 

Query: 4   ISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIG-IQLTMEELESFLERPSLDFDEFLFF 63
           I++ DL R+FK LDKN DGL+ L EL W+LD +G  + T +ELE  + + SLD DEFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 64  YESISKQNKGECKGGVAGCVQDNDNDQDQDDMEIVYLAFKVFDMNGDGFISCDELENVLV 123
           Y      +KG  K      V DND        E +  AF VFD+NGDG+IS +EL +VL 
Sbjct: 66  YYDAVLDSKGSKKN--IDVVADND--------EAIARAFNVFDVNGDGYISAEELRDVLE 125

Query: 124 KL------ELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMML 163
           +L      + WD        C  MIR +D NLDG +DFEEFKNM+L
Sbjct: 126 RLGFEEEAKAWD--------CGRMIRVHDKNLDGFVDFEEFKNMIL 153

[Close]
BLAST of CSPI06G19980 vs. Swiss-Prot
Match: CML43_ARATH (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana GN=CML43 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.7e-18
Identity = 59/166 (35.54%), Postives = 92/166 (55.42%), Query Frame = 1 

Query: 2   PPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFL------ERPSLD 61
           P +++  LHR+F   DKN DG I ++EL+  L  +G+     +L+S +      ++  L 
Sbjct: 22  PSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLR 81

Query: 62  FDEFLFFYESISKQNKGECKGGVAGCVQDNDNDQDQDDMEIVYLAFKVFDMNGDGFISCD 121
           FD+F   ++++ +       GG   C  D   + D ++      AF VFD +GDGFIS  
Sbjct: 82  FDDFAALHKTLDE----SFFGGEGSCC-DGSPESDLEE------AFNVFDEDGDGFISAV 141

Query: 122 ELENVLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
           EL+ VL KL L +A   +++    MI + D+N DG++DF EFKNMM
Sbjct: 142 ELQKVLKKLGLPEA--GEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

[Close]
BLAST of CSPI06G19980 vs. Swiss-Prot
Match: CML28_ORYSJ (Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica GN=CML28 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.1e-17
Identity = 58/164 (35.37%), Postives = 84/164 (51.22%), Query Frame = 1 

Query: 4   ISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLER------PSLDFD 63
           + S +L ++FK  DKN DG I  +EL     + GI +  +EL++ +++        +D +
Sbjct: 1   MDSTELRKVFKMFDKNGDGRITKKELGESFKNFGIFIPDDELDATMDKIDANGDGCVDVE 60

Query: 64  EFLFFYESISKQNKGECKGGVAGCVQDNDNDQDQDDMEIVYLAFKVFDMNGDGFISCDEL 123
           EF   Y SI   +        A      +     D+ E +  AF VFD NGDGFI+ DEL
Sbjct: 61  EFGLLYRSILGDDAAGRAPRTAAAAIGGEGGAPDDEDEGMREAFNVFDQNGDGFITVDEL 120

Query: 124 ENVLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
            +VL  L L     +  D CR MI   D + DG++DF+EFK MM
Sbjct: 121 RSVLSSLGLKHGRTA--DDCRRMISMVDADGDGRVDFKEFKQMM 162

[Close]
BLAST of CSPI06G19980 vs. Swiss-Prot
Match: ALLB3_BETPN (Calcium-binding allergen Bet v 3 OS=Betula pendula GN=BETVIII PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 3.2e-17
Identity = 60/168 (35.71%), Postives = 93/168 (55.36%), Query Frame = 1 

Query: 9   LHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLE------RPSLDFDEFLFF 68
           L RIF   DKN DG+I + EL+  L+ +G++  + ELES ++         L F++F+  
Sbjct: 41  LRRIFDLFDKNSDGIITVDELSRALNLLGLETDLSELESTVKSFTREGNIGLQFEDFISL 100

Query: 69  YESISKQNKGECKGGVAGCVQDNDNDQDQDDMEIVYLA---------FKVFDMNGDGFIS 128
           ++S++  +     GG        D D +++DM    L+         FKVFD +GDG+IS
Sbjct: 101 HQSLN--DSYFAYGG-------EDEDDNEEDMRKSILSQEEADSFGGFKVFDEDGDGYIS 160

Query: 129 CDELENVLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
             EL+ VL KL    +  S++D    MI + D+N DG++DF EFK+MM
Sbjct: 161 ARELQMVLGKLGF--SEGSEIDRVEKMIVSVDSNRDGRVDFFEFKDMM 197

[Close]
BLAST of CSPI06G19980 vs. Swiss-Prot
Match: CAST_SOLTU (Calcium-binding protein CAST OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 7.1e-17
Identity = 63/169 (37.28%), Postives = 96/169 (56.80%), Query Frame = 1 

Query: 2   PPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFL------ERPSLD 61
           P ++S  L RIF   D+N D LI ++EL+  L+ +G+   + E+ES +      E   L 
Sbjct: 34  PSLNSIRLRRIFDVFDRNHDCLISVEELSQALNLLGLDADLSEIESMVKLHIKPENTGLR 93

Query: 62  FDEFLFFYESISKQ---NKGECKGGVAGCVQDNDNDQDQDDMEIVYLAFKVFDMNGDGFI 121
           F++F   + S++     +K E K G+     + D  QD+ D++    AF VFD NGDGFI
Sbjct: 94  FEDFETLHRSLNDVFFGSKCEDKLGL-----NPDPAQDESDLKE---AFDVFDENGDGFI 153

Query: 122 SCDELENVLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
           S  EL+ VL KL L +   S++D    MI + + + DG++DF EFK+MM
Sbjct: 154 SAKELQVVLEKLGLPEG--SEIDRVEMMISSVEQDHDGRVDFFEFKDMM 192

[Close]
BLAST of CSPI06G19980 vs. TrEMBL
Match: A0A0A0KFQ6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G376250 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 1.5e-90
Identity = 164/165 (99.39%), Postives = 164/165 (99.39%), Query Frame = 1 

Query: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60
           MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL
Sbjct: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQDQDDMEIVYLAFKVFDMNGDGFISCDELENV 120
           FFYESISKQNKGECKGGVAGCVQDNDNDQ QDDMEIVYLAFKVFDMNGDGFISCDELENV
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120

Query: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 165

[Close]
BLAST of CSPI06G19980 vs. TrEMBL
Match: W9RRJ8_9ROSA (Putative calcium-binding protein CML44 OS=Morus notabilis GN=L484_003448 PE=4 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 1.6e-44
Identity = 98/168 (58.33%), Postives = 126/168 (75.00%), Query Frame = 1 

Query: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQL---TMEELESFLERPSLDFD 60
           M  +S+ DL+RIFKKLDKN DGL+ + ELNWLL+ IG+ +   ++ ELES + + SL FD
Sbjct: 1   MCSLSANDLNRIFKKLDKNGDGLVSVDELNWLLERIGVDIQLISLTELESLVGKSSLSFD 60

Query: 61  EFLFFYESISKQNKGECKGGVAGCVQDNDNDQDQDDMEIVYLAFKVFDMNGDGFISCDEL 120
           EFLFFY+SIS  N GE KGG     QD+D+D D +D ++V  AFKVFD+N DGFISC+EL
Sbjct: 61  EFLFFYKSISNPNNGESKGGDIDQDQDHDHDHDGEDSDLV-KAFKVFDINDDGFISCEEL 120

Query: 121 ENVLVKLELW-DASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLT 165
           ++VL++L L  D + S  D C+SMI A+D N DG+LDFEEFKNMMLLT
Sbjct: 121 QSVLIRLGLMEDHNSSGRDNCKSMIGAFDANSDGQLDFEEFKNMMLLT 167

[Close]
BLAST of CSPI06G19980 vs. TrEMBL
Match: A0A061FHR5_THECC (Calcium-binding EF-hand family protein OS=Theobroma cacao GN=TCM_035222 PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 2.8e-44
Identity = 99/164 (60.37%), Postives = 123/164 (75.00%), Query Frame = 1 

Query: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60
           M P+S  DL RIF+KLDKN DGL+ L+ELNWLL  IG+Q ++EEL SF+ +P LDF+EFL
Sbjct: 29  MSPLSKNDLQRIFEKLDKNGDGLVSLEELNWLLVRIGVQFSLEELGSFVGKPCLDFNEFL 88

Query: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQDQDDMEIVYLAFKVFDMNGDGFISCDELENV 120
           FFY+SIS QN        AG  +++   Q  +D ++V  AFKVFD+NGDGFISC+EL++V
Sbjct: 89  FFYDSISNQNDDGKNMEPAGSGEEDGLIQADEDSDLV-KAFKVFDLNGDGFISCEELQSV 148

Query: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLT 165
           LV+L LWD S S  D CR+MI  YDTNLDG +DFEEFKNMML T
Sbjct: 149 LVRLGLWDES-SGKD-CRNMICFYDTNLDGMVDFEEFKNMMLHT 189

[Close]
BLAST of CSPI06G19980 vs. TrEMBL
Match: A0A0D2MEI7_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_002G223000 PE=4 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 6.8e-43
Identity = 96/173 (55.49%), Postives = 120/173 (69.36%), Query Frame = 1 

Query: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60
           M P+S  DL RIF+KLDKN DGL+ L+ELNWLL+ IG+Q ++EELES + +P L F+EFL
Sbjct: 1   MSPLSKNDLRRIFEKLDKNGDGLVSLEELNWLLERIGVQFSLEELESLVGKPCLGFEEFL 60

Query: 61  FFYESISKQN-----------KGECKGGVAGCVQDNDNDQDQDDMEIVYLAFKVFDMNGD 120
           FFY+SIS  N           + E    V G   D+D+D D  D ++   AFKVFD+NGD
Sbjct: 61  FFYDSISNHNTKIEAAFDDEEEEEEIRMVHGGGDDDDDDDDHGDSDLA-KAFKVFDLNGD 120

Query: 121 GFISCDELENVLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMML 163
           GFISC+EL++VLV+L LWD        CR+MI  YDTNLDG +DFEEFKNMML
Sbjct: 121 GFISCEELQSVLVRLGLWDEKNG--KDCRNMICFYDTNLDGMIDFEEFKNMML 170

[Close]
BLAST of CSPI06G19980 vs. TrEMBL
Match: V4T321_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10002693mg PE=4 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 4.4e-42
Identity = 93/169 (55.03%), Postives = 117/169 (69.23%), Query Frame = 1 

Query: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60
           M P+++ DL RIF+KLD+N DGL+ L+ELNW+L+ IG+  T+EELE+ +E+PSL FDEFL
Sbjct: 1   MAPLTANDLQRIFEKLDRNGDGLVSLEELNWVLERIGVHFTLEELEALVEKPSLCFDEFL 60

Query: 61  FFYESISKQNKGECKGGVAGCVQDNDN--DQDQDDMEI--VYLAFKVFDMNGDGFISCDE 120
           FF +SI   N        A   Q+ D   D D  D EI  +Y AF VFD+NGDGFI+ +E
Sbjct: 61  FFCDSIISNNNNNNPNAAADASQNADEAADDDSGDEEISDLYKAFSVFDVNGDGFITSEE 120

Query: 121 LENVLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           L++VL +L LWD   S    CRSMI  YDTN DG LDFEEFKNMML +T
Sbjct: 121 LQSVLARLGLWDEKSS--QDCRSMIYVYDTNSDGVLDFEEFKNMMLRST 167

[Close]
BLAST of CSPI06G19980 vs. TAIR10
Match: AT1G21550.1 (AT1G21550.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 127.9 bits (320), Expect = 5.9e-30
Identity = 77/166 (46.39%), Postives = 98/166 (59.04%), Query Frame = 1 

Query: 4   ISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIG-IQLTMEELESFLERPSLDFDEFLFF 63
           I++ DL R+FK LDKN DGL+ L EL W+LD +G  + T +ELE  + + SLD DEFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 64  YESISKQNKGECKGGVAGCVQDNDNDQDQDDMEIVYLAFKVFDMNGDGFISCDELENVLV 123
           Y      +KG  K      V DND        E +  AF VFD+NGDG+IS +EL +VL 
Sbjct: 66  YYDAVLDSKGSKKN--IDVVADND--------EAIARAFNVFDVNGDGYISAEELRDVLE 125

Query: 124 KL------ELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMML 163
           +L      + WD        C  MIR +D NLDG +DFEEFKNM+L
Sbjct: 126 RLGFEEEAKAWD--------CGRMIRVHDKNLDGFVDFEEFKNMIL 153

[Close]
BLAST of CSPI06G19980 vs. TAIR10
Match: AT4G20780.1 (AT4G20780.1 calmodulin like 42)

HSP 1 Score: 97.1 bits (240), Expect = 1.1e-20
Identity = 61/166 (36.75%), Postives = 95/166 (57.23%), Query Frame = 1 

Query: 2   PPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLE------RPSLD 61
           P +++  L RIF   DKN DG I ++EL+  L  +G+   + +L+S +E         L+
Sbjct: 23  PSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLN 82

Query: 62  FDEFLFFYESISKQNKGECKGGVAGCVQDNDNDQDQDDMEIVYLAFKVFDMNGDGFISCD 121
           FD+F   ++++     G   GG  G  +D+ +   +++ ++   AFKVFD NGDGFIS  
Sbjct: 83  FDDFSSLHKTLDDSFFGGACGG--GENEDDPSSAAENESDLAE-AFKVFDENGDGFISAR 142

Query: 122 ELENVLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
           EL+ VL KL L +    +++    MI + D N DG++DF EFKNMM
Sbjct: 143 ELQTVLKKLGLPEG--GEMERVEKMIVSVDRNQDGRVDFFEFKNMM 183

[Close]
BLAST of CSPI06G19980 vs. TAIR10
Match: AT5G44460.1 (AT5G44460.1 calmodulin like 43)

HSP 1 Score: 94.0 bits (232), Expect = 9.5e-20
Identity = 59/166 (35.54%), Postives = 92/166 (55.42%), Query Frame = 1 

Query: 2   PPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFL------ERPSLD 61
           P +++  LHR+F   DKN DG I ++EL+  L  +G+     +L+S +      ++  L 
Sbjct: 22  PSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLR 81

Query: 62  FDEFLFFYESISKQNKGECKGGVAGCVQDNDNDQDQDDMEIVYLAFKVFDMNGDGFISCD 121
           FD+F   ++++ +       GG   C  D   + D ++      AF VFD +GDGFIS  
Sbjct: 82  FDDFAALHKTLDE----SFFGGEGSCC-DGSPESDLEE------AFNVFDEDGDGFISAV 141

Query: 122 ELENVLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
           EL+ VL KL L +A   +++    MI + D+N DG++DF EFKNMM
Sbjct: 142 ELQKVLKKLGLPEA--GEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

[Close]
BLAST of CSPI06G19980 vs. TAIR10
Match: AT1G05990.1 (AT1G05990.1 EF hand calcium-binding protein family)

HSP 1 Score: 87.4 bits (215), Expect = 8.9e-18
Identity = 56/160 (35.00%), Postives = 88/160 (55.00%), Query Frame = 1 

Query: 8   DLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSL------DFDEFLF 67
           +L R+F+  DKN DG I  +EL+  L S+GI +  +EL   +E+  +      D DEF  
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGE 64

Query: 68  FYESISKQNKGECKGGVAGCVQDNDNDQDQDDMEIVYLAFKVFDMNGDGFISCDELENVL 127
            Y++I                  ++ D++++DM+    AF VFD NGDGFI+ DEL+ VL
Sbjct: 65  LYKTIM-----------------DEEDEEEEDMKE---AFNVFDQNGDGFITVDELKAVL 124

Query: 128 VKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
             L L       +D C+ MI+  D + DG+++++EF+ MM
Sbjct: 125 SSLGL--KQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMM 142

[Close]
BLAST of CSPI06G19980 vs. TAIR10
Match: AT2G43290.1 (AT2G43290.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 86.7 bits (213), Expect = 1.5e-17
Identity = 54/164 (32.93%), Postives = 88/164 (53.66%), Query Frame = 1 

Query: 4   ISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLER------PSLDFD 63
           I   +L R+F+  DKN DG I  +ELN  L+++GI +  ++L   + +        +D D
Sbjct: 61  IDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDID 120

Query: 64  EFLFFYESISKQNKGECKGGVAGCVQDNDNDQDQDDMEIVYLAFKVFDMNGDGFISCDEL 123
           EF   Y SI  ++              ND + +++DM+    AF VFD +GDGFI+ +EL
Sbjct: 121 EFESLYSSIVDEH-------------HNDGETEEEDMKD---AFNVFDQDGDGFITVEEL 180

Query: 124 ENVLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
           ++V+  L L       +D C+ MI   D + DG+++++EF  MM
Sbjct: 181 KSVMASLGL--KQGKTLDGCKKMIMQVDADGDGRVNYKEFLQMM 206

[Close]
BLAST of CSPI06G19980 vs. NCBI nr
Match: gi|449463432|ref|XP_004149438.1| (PREDICTED: probable calcium-binding protein CML44 [Cucumis sativus])

HSP 1 Score: 340.1 bits (871), Expect = 2.2e-90
Identity = 164/165 (99.39%), Postives = 164/165 (99.39%), Query Frame = 1 

Query: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60
           MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL
Sbjct: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQDQDDMEIVYLAFKVFDMNGDGFISCDELENV 120
           FFYESISKQNKGECKGGVAGCVQDNDNDQ QDDMEIVYLAFKVFDMNGDGFISCDELENV
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120

Query: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 165

[Close]
BLAST of CSPI06G19980 vs. NCBI nr
Match: gi|659081990|ref|XP_008441608.1| (PREDICTED: probable calcium-binding protein CML44 [Cucumis melo])

HSP 1 Score: 306.6 bits (784), Expect = 2.6e-80
Identity = 148/165 (89.70%), Postives = 156/165 (94.55%), Query Frame = 1 

Query: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60
           M PISSKDLHRIFKKLDKNCDGL+CLQEL WLLD IGIQLTMEELESFLE+PSLDFDEFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQDQDDMEIVYLAFKVFDMNGDGFISCDELENV 120
           FFYESISKQNKGECKGGVAGCVQD+     +DD EIVY+AFKVFDMNGDGFISCDELENV
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDD----HEDDTEIVYMAFKVFDMNGDGFISCDELENV 120

Query: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           LV+LELWDASRS+VDYCRSMI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

[Close]
BLAST of CSPI06G19980 vs. NCBI nr
Match: gi|703133631|ref|XP_010105440.1| (putative calcium-binding protein CML44 [Morus notabilis])

HSP 1 Score: 187.2 bits (474), Expect = 2.3e-44
Identity = 98/168 (58.33%), Postives = 126/168 (75.00%), Query Frame = 1 

Query: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQL---TMEELESFLERPSLDFD 60
           M  +S+ DL+RIFKKLDKN DGL+ + ELNWLL+ IG+ +   ++ ELES + + SL FD
Sbjct: 1   MCSLSANDLNRIFKKLDKNGDGLVSVDELNWLLERIGVDIQLISLTELESLVGKSSLSFD 60

Query: 61  EFLFFYESISKQNKGECKGGVAGCVQDNDNDQDQDDMEIVYLAFKVFDMNGDGFISCDEL 120
           EFLFFY+SIS  N GE KGG     QD+D+D D +D ++V  AFKVFD+N DGFISC+EL
Sbjct: 61  EFLFFYKSISNPNNGESKGGDIDQDQDHDHDHDGEDSDLV-KAFKVFDINDDGFISCEEL 120

Query: 121 ENVLVKLELW-DASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLT 165
           ++VL++L L  D + S  D C+SMI A+D N DG+LDFEEFKNMMLLT
Sbjct: 121 QSVLIRLGLMEDHNSSGRDNCKSMIGAFDANSDGQLDFEEFKNMMLLT 167

[Close]
BLAST of CSPI06G19980 vs. NCBI nr
Match: gi|590599643|ref|XP_007019238.1| (Calcium-binding EF-hand family protein [Theobroma cacao])

HSP 1 Score: 186.4 bits (472), Expect = 4.0e-44
Identity = 99/164 (60.37%), Postives = 123/164 (75.00%), Query Frame = 1 

Query: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60
           M P+S  DL RIF+KLDKN DGL+ L+ELNWLL  IG+Q ++EEL SF+ +P LDF+EFL
Sbjct: 29  MSPLSKNDLQRIFEKLDKNGDGLVSLEELNWLLVRIGVQFSLEELGSFVGKPCLDFNEFL 88

Query: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQDQDDMEIVYLAFKVFDMNGDGFISCDELENV 120
           FFY+SIS QN        AG  +++   Q  +D ++V  AFKVFD+NGDGFISC+EL++V
Sbjct: 89  FFYDSISNQNDDGKNMEPAGSGEEDGLIQADEDSDLV-KAFKVFDLNGDGFISCEELQSV 148

Query: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLT 165
           LV+L LWD S S  D CR+MI  YDTNLDG +DFEEFKNMML T
Sbjct: 149 LVRLGLWDES-SGKD-CRNMICFYDTNLDGMVDFEEFKNMMLHT 189

[Close]
BLAST of CSPI06G19980 vs. NCBI nr
Match: gi|823136399|ref|XP_012467960.1| (PREDICTED: probable calcium-binding protein CML44 [Gossypium raimondii])

HSP 1 Score: 181.8 bits (460), Expect = 9.8e-43
Identity = 96/173 (55.49%), Postives = 120/173 (69.36%), Query Frame = 1 

Query: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60
           M P+S  DL RIF+KLDKN DGL+ L+ELNWLL+ IG+Q ++EELES + +P L F+EFL
Sbjct: 1   MSPLSKNDLRRIFEKLDKNGDGLVSLEELNWLLERIGVQFSLEELESLVGKPCLGFEEFL 60

Query: 61  FFYESISKQN-----------KGECKGGVAGCVQDNDNDQDQDDMEIVYLAFKVFDMNGD 120
           FFY+SIS  N           + E    V G   D+D+D D  D ++   AFKVFD+NGD
Sbjct: 61  FFYDSISNHNTKIEAAFDDEEEEEEIRMVHGGGDDDDDDDDHGDSDLA-KAFKVFDLNGD 120

Query: 121 GFISCDELENVLVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMML 163
           GFISC+EL++VLV+L LWD        CR+MI  YDTNLDG +DFEEFKNMML
Sbjct: 121 GFISCEELQSVLVRLGLWDEKNG--KDCRNMICFYDTNLDGMIDFEEFKNMML 170

The following BLAST results are available for this feature:
BLAST of CSPI06G19980 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML44_ARATH1.1e-2846.39Probable calcium-binding protein CML44 OS=Arabidopsis thaliana GN=CML44 PE=2 SV=... [more]
CML43_ARATH1.7e-1835.54Probable calcium-binding protein CML43 OS=Arabidopsis thaliana GN=CML43 PE=2 SV=... [more]
CML28_ORYSJ1.1e-1735.37Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica GN=CML28 ... [more]
ALLB3_BETPN3.2e-1735.71Calcium-binding allergen Bet v 3 OS=Betula pendula GN=BETVIII PE=1 SV=1[more]
CAST_SOLTU7.1e-1737.28Calcium-binding protein CAST OS=Solanum tuberosum PE=2 SV=1[more]
BLAST of CSPI06G19980 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KFQ6_CUCSA1.5e-9099.39Uncharacterized protein OS=Cucumis sativus GN=Csa_6G376250 PE=4 SV=1[more]
W9RRJ8_9ROSA1.6e-4458.33Putative calcium-binding protein CML44 OS=Morus notabilis GN=L484_003448 PE=4 SV... [more]
A0A061FHR5_THECC2.8e-4460.37Calcium-binding EF-hand family protein OS=Theobroma cacao GN=TCM_035222 PE=4 SV=... [more]
A0A0D2MEI7_GOSRA6.8e-4355.49Uncharacterized protein OS=Gossypium raimondii GN=B456_002G223000 PE=4 SV=1[more]
V4T321_9ROSI4.4e-4255.03Uncharacterized protein OS=Citrus clementina GN=CICLE_v10002693mg PE=4 SV=1[more]
BLAST of CSPI06G19980 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G21550.15.9e-3046.39 Calcium-binding EF-hand family protein[more]
AT4G20780.11.1e-2036.75 calmodulin like 42[more]
AT5G44460.19.5e-2035.54 calmodulin like 43[more]
AT1G05990.18.9e-1835.00 EF hand calcium-binding protein family[more]
AT2G43290.11.5e-1732.93 Calcium-binding EF-hand family protein[more]
BLAST of CSPI06G19980 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449463432|ref|XP_004149438.1|2.2e-9099.39PREDICTED: probable calcium-binding protein CML44 [Cucumis sativus][more]
gi|659081990|ref|XP_008441608.1|2.6e-8089.70PREDICTED: probable calcium-binding protein CML44 [Cucumis melo][more]
gi|703133631|ref|XP_010105440.1|2.3e-4458.33putative calcium-binding protein CML44 [Morus notabilis][more]
gi|590599643|ref|XP_007019238.1|4.0e-4460.37Calcium-binding EF-hand family protein [Theobroma cacao][more]
gi|823136399|ref|XP_012467960.1|9.8e-4355.49PREDICTED: probable calcium-binding protein CML44 [Gossypium raimondii][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G19980.1CSPI06G19980.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 12..29
score: 0
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 98..161
score: 1.
IPR002048EF-hand domainSMARTSM00054efh_1coord: 96..124
score: 0.19coord: 136..164
score: 0.0051coord: 8..36
score: 0
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 132..165
score: 13.649coord: 92..127
score: 12.812coord: 4..39
score: 11
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 108..161
score: 1.2E-16coord: 60..107
score: 1.2E-4coord: 7..59
score: 5.
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 7..162
score: 7.0
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 105..117
score: -coord: 17..29
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 1..67
score: 2.0E-48coord: 88..162
score: 2.0
NoneNo IPR availablePANTHERPTHR10891:SF530SUBFAMILY NOT NAMEDcoord: 1..67
score: 2.0E-48coord: 88..162
score: 2.0