MELO3C008466.2 (gene) Melon (DHL92) v3.6.1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	MELO3C008466.2
Type	gene
Organism	Cucumis melo (Melon (DHL92) v3.6.1)
Description	Calcium-binding family protein
Location	chr03 : 7094344 .. 7095141 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTGACAACAATAATTCCACACGTATTATATATTTTGGTAATAGTCTTTCTTGTGTGTTTACATTTCAATTCTAAACCGAAGAACAAAAAGCACCATGTCTCCAATTAGCTCCAAAGATTTGCATAGGATCTTCAAGAAGCTAGACAAGAACTGTGATGGCCTCCTCTGCCTACAAGAGCTCAAGTGGCTCTTGGATAGGATTGGCATCCAATTAACCATGGAGGAGCTGGAATCATTTCTAGAGAAACCCAGCCTCGACTTCGACGAGTTCTTGTTCTTTTACGAGTCAATATCAAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGTGGCGGGGTGTGTGCAAGATGATCATGAGGATGATACTGAGATTGTATATATGGCTTTTAAAGTGTTTGACATGAATGGTGATGGCTTCATTTCTTGCGACGAGCTCGAAAACGTGTTGGTGAGGCTCGAGCTTTGGGATGCAAGTAGAAGCGAGGTAGATTATTGTAGAAGCATGATCCAAGCTTATGACACCAATTTGGATGGAAAGCTGGATTTTGAGGAGTTTAAGAACATGATGTTGCTCACAACATAGTTCTTCTTTTAGAGTTTCAAAAAAGAAAAGGAAAAAAAAAAAAAATCCCCTTTTTTGTTATATTGTTGTTAAATATAGATATTCGTTTGGTCTGTTTATTTGCAGGTACAATTTGAGAAGCTGGGAGGCTTCAAGAATAAAAATTTTGGTCATGGAGTTCATGTAATTATATTTTTGTTGCTCCAATCAGATTATGTATTTCCACTCTCCTCTATAA

mRNA sequence

ATTGACAACAATAATTCCACACGTATTATATATTTTGGTAATAGTCTTTCTTGTGTGTTTACATTTCAATTCTAAACCGAAGAACAAAAAGCACCATGTCTCCAATTAGCTCCAAAGATTTGCATAGGATCTTCAAGAAGCTAGACAAGAACTGTGATGGCCTCCTCTGCCTACAAGAGCTCAAGTGGCTCTTGGATAGGATTGGCATCCAATTAACCATGGAGGAGCTGGAATCATTTCTAGAGAAACCCAGCCTCGACTTCGACGAGTTCTTGTTCTTTTACGAGTCAATATCAAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGTGGCGGGGTGTGTGCAAGATGATCATGAGGATGATACTGAGATTGTATATATGGCTTTTAAAGTGTTTGACATGAATGGTGATGGCTTCATTTCTTGCGACGAGCTCGAAAACGTGTTGGTGAGGCTCGAGCTTTGGGATGCAAGTAGAAGCGAGGTAGATTATTGTAGAAGCATGATCCAAGCTTATGACACCAATTTGGATGGAAAGCTGGATTTTGAGGAGTTTAAGAACATGATGTTGCTCACAACATAGTTCTTCTTTTAGAGTTTCAAAAAAGAAAAGGAAAAAAAAAAAAAATCCCCTTTTTTGTTATATTGTTGTTAAATATAGATATTCGTTTGGTCTGTTTATTTGCAGGTACAATTTGAGAAGCTGGGAGGCTTCAAGAATAAAAATTTTGGTCATGGAGTTCATGTAATTATATTTTTGTTGCTCCAATCAGATTATGTATTTCCACTCTCCTCTATAA

Coding sequence (CDS)

ATGTCTCCAATTAGCTCCAAAGATTTGCATAGGATCTTCAAGAAGCTAGACAAGAACTGTGATGGCCTCCTCTGCCTACAAGAGCTCAAGTGGCTCTTGGATAGGATTGGCATCCAATTAACCATGGAGGAGCTGGAATCATTTCTAGAGAAACCCAGCCTCGACTTCGACGAGTTCTTGTTCTTTTACGAGTCAATATCAAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGTGGCGGGGTGTGTGCAAGATGATCATGAGGATGATACTGAGATTGTATATATGGCTTTTAAAGTGTTTGACATGAATGGTGATGGCTTCATTTCTTGCGACGAGCTCGAAAACGTGTTGGTGAGGCTCGAGCTTTGGGATGCAAGTAGAAGCGAGGTAGATTATTGTAGAAGCATGATCCAAGCTTATGACACCAATTTGGATGGAAAGCTGGATTTTGAGGAGTTTAAGAACATGATGTTGCTCACAACATAG

Protein sequence

MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFLFFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT

Homology

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BLAST of MELO3C008466.2 vs. NCBI nr
Match: XP_008441608.1 (PREDICTED: probable calcium-binding protein CML44 [Cucumis melo])

HSP 1 Score: 333.2 bits (853), Expect = 5.0e-88
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60
           MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120
           FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120

Query: 121 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 162
           ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

[Close]

BLAST of MELO3C008466.2 vs. NCBI nr
Match: XP_004149438.1 (PREDICTED: probable calcium-binding protein CML44 [Cucumis sativus] >KGN47684.1 hypothetical protein Csa_6G376250 [Cucumis sativus])

HSP 1 Score: 305.4 bits (781), Expect = 1.1e-79
Identity = 148/165 (89.70%), Postives = 156/165 (94.55%), Query Frame = 0


Query: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60
           M PISSKDLHRIFKKLDKNCDGL+CLQEL WLLD IGIQLTMEELESFLE+PSLDFDEFL
Sbjct: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVAGCVQDDH----EDDTEIVYMAFKVFDMNGDGFISCDELENV 120
           FFYESISKQNKGECKGGVAGCVQD+     +DD EIVY+AFKVFDMNGDGFISCDELENV
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120

Query: 121 LVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 162
           LV+LELWDASRS+VDYCRSMI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 165

[Close]

BLAST of MELO3C008466.2 vs. NCBI nr
Match: XP_023551499.1 (probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 263.5 bits (672), Expect = 4.8e-67
Identity = 132/158 (83.54%), Postives = 141/158 (89.24%), Query Frame = 0


Query: 3   PISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFLFF 62
           PISS DL RIF +LDKN DGL+C+QELKWLLD IGIQLTMEELESFLEKPSLDFDEFLFF
Sbjct: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63

Query: 63  YESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 122
           YESISKQNKGECKGG+      D E+D EIVYMAFKVFD+NGDGFISC+ELENVL RLEL
Sbjct: 64  YESISKQNKGECKGGLVD-EHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 123

Query: 123 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLT 161
           WDA+RSEVD CRSMI+AYDTNLDGKLDFEEF NMML T
Sbjct: 124 WDANRSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

[Close]

BLAST of MELO3C008466.2 vs. NCBI nr
Match: XP_022939513.1 (probable calcium-binding protein CML44 [Cucurbita moschata])

HSP 1 Score: 262.3 bits (669), Expect = 1.1e-66
Identity = 131/158 (82.91%), Postives = 141/158 (89.24%), Query Frame = 0


Query: 3   PISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFLFF 62
           PISS DL RIF +LDKN DGL+C+QELKWLLD IGIQLTMEELESFLEKPSLDFDEFLFF
Sbjct: 4   PISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFF 63

Query: 63  YESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 122
           YESISKQNKGECKGG+      D E+D EIVYMAFKVFD+NGDGFISC+ELENVL RLEL
Sbjct: 64  YESISKQNKGECKGGLVD-EHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 123

Query: 123 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLT 161
           WDA++SEVD CRSMI+AYDTNLDGKLDFEEF NMML T
Sbjct: 124 WDANKSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

[Close]

BLAST of MELO3C008466.2 vs. NCBI nr
Match: XP_022993348.1 (probable calcium-binding protein CML44 [Cucurbita maxima])

HSP 1 Score: 260.4 bits (664), Expect = 4.1e-66
Identity = 132/161 (81.99%), Postives = 142/161 (88.20%), Query Frame = 0


Query: 3   PISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFLFF 62
           PISS DL RIF +LDKN DGL+C+QELKWLLD IGIQLTMEELESFLEKPSLDFDEFL F
Sbjct: 4   PISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLIF 63

Query: 63  YESISKQNKGECKGGVAGCVQDDHED---DTEIVYMAFKVFDMNGDGFISCDELENVLVR 122
           YES+SKQNKGECKGG+     D+HED   D EIVYMAFKVFD+NGDGFISC+ELENVL R
Sbjct: 64  YESMSKQNKGECKGGLV----DEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSR 123

Query: 123 LELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLT 161
           LELWDA+RSEVD CRSMI+AYDTNLDGKLDFEEF NMML T
Sbjct: 124 LELWDANRSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

[Close]

BLAST of MELO3C008466.2 vs. TAIR10
Match: AT1G21550.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 127.5 bits (319), Expect = 7.5e-30
Identity = 74/156 (47.44%), Postives = 99/156 (63.46%), Query Frame = 0


Query: 4   ISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIG-IQLTMEELESFLEKPSLDFDEFLFF 63
           I++ DL R+FK LDKN DGL+ L EL W+LD++G  + T +ELE  + K SLD DEFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 64  YESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 123
           Y      +KG  K        D   D+ E +  AF VFD+NGDG+IS +EL +VL RL  
Sbjct: 66  YYDAVLDSKGSKKN------IDVVADNDEAIARAFNVFDVNGDGYISAEELRDVLERLGF 125

Query: 124 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMML 159
            + +++    C  MI+ +D NLDG +DFEEFKNM+L
Sbjct: 126 EEEAKAW--DCGRMIRVHDKNLDGFVDFEEFKNMIL 153

[Close]

BLAST of MELO3C008466.2 vs. TAIR10
Match: AT5G44460.1 (calmodulin like 43)

HSP 1 Score: 98.6 bits (244), Expect = 3.7e-21
Identity = 60/155 (38.71%), Postives = 86/155 (55.48%), Query Frame = 0


Query: 9   LHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFL------EKPSLDFDEFLFF 68
           LHR+F   DKN DG + ++EL   L R+G+     +L+S +      +K  L FD+F   
Sbjct: 29  LHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFDDFAAL 88

Query: 69  YESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 128
           ++++ +       GG   C     E D E    AF VFD +GDGFIS  EL+ VL +L L
Sbjct: 89  HKTLDE----SFFGGEGSCCDGSPESDLE---EAFNVFDEDGDGFISAVELQKVLKKLGL 148

Query: 129 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
            +A   E++    MI + D+N DG++DF EFKNMM
Sbjct: 149 PEA--GEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

[Close]

BLAST of MELO3C008466.2 vs. TAIR10
Match: AT4G20780.1 (calmodulin like 42)

HSP 1 Score: 88.2 bits (217), Expect = 5.0e-18
Identity = 57/157 (36.31%), Postives = 85/157 (54.14%), Query Frame = 0


Query: 9   LHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLE------KPSLDFDEFLFF 68
           L RIF   DKN DG + ++EL   L R+G+   + +L+S +E         L+FD+F   
Sbjct: 30  LQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFDDFSSL 89

Query: 69  YESI--SKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 128
           ++++  S                   E+++++   AFKVFD NGDGFIS  EL+ VL +L
Sbjct: 90  HKTLDDSXXXXXXXXXXXXXXXXXXXENESDLA-EAFKVFDENGDGFISARELQTVLKKL 149

Query: 129 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
            L +    E++    MI + D N DG++DF EFKNMM
Sbjct: 150 GLPEG--GEMERVEKMIVSVDRNQDGRVDFFEFKNMM 183

[Close]

BLAST of MELO3C008466.2 vs. TAIR10
Match: AT4G12860.1 (EF hand calcium-binding protein family)

HSP 1 Score: 80.1 bits (196), Expect = 1.4e-15
Identity = 51/156 (32.69%), Postives = 80/156 (51.28%), Query Frame = 0


Query: 8   DLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEK------PSLDFDEFLF 67
           +L R+F+  DKN DG +   ELK     +GI +   E+   + K       ++D DEF  
Sbjct: 5   ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGS 64

Query: 68  FYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLE 127
            Y+ + ++             +++ ED  E    AF+VFD NGDGFI+ +EL +VL  + 
Sbjct: 65  LYQEMVEE-------------KEEEEDMRE----AFRVFDQNGDGFITDEELRSVLASMG 124

Query: 128 LWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
           L       ++ C+ MI   D + DG ++F+EFK MM
Sbjct: 125 L--KQGRTLEDCKKMISKVDVDGDGMVNFKEFKQMM 141

[Close]

BLAST of MELO3C008466.2 vs. TAIR10
Match: AT1G24620.1 (EF hand calcium-binding protein family)

HSP 1 Score: 79.0 bits (193), Expect = 3.1e-15
Identity = 51/153 (33.33%), Postives = 76/153 (49.67%), Query Frame = 0


Query: 7   KDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFLFFYESI 66
           ++L  +FKK D N DG +  +EL  ++  +G ++  EELE  + +     D ++ F E +
Sbjct: 36  RELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFV 95

Query: 67  SKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLELWDAS 126
               KG            D  D  E +  AF V+D++G+G IS +EL  VL  L      
Sbjct: 96  ELNTKGM-----------DQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSL----GD 155

Query: 127 RSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLL 160
              +  CR MI   D + DG +DFEEFK MM +
Sbjct: 156 ECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTM 173

[Close]

BLAST of MELO3C008466.2 vs. Swiss-Prot
Match: sp|Q9LPK5|CML44_ARATH (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 PE=2 SV=2)

HSP 1 Score: 127.5 bits (319), Expect = 1.4e-28
Identity = 74/156 (47.44%), Postives = 99/156 (63.46%), Query Frame = 0


Query: 4   ISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIG-IQLTMEELESFLEKPSLDFDEFLFF 63
           I++ DL R+FK LDKN DGL+ L EL W+LD++G  + T +ELE  + K SLD DEFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 64  YESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 123
           Y      +KG  K        D   D+ E +  AF VFD+NGDG+IS +EL +VL RL  
Sbjct: 66  YYDAVLDSKGSKKN------IDVVADNDEAIARAFNVFDVNGDGYISAEELRDVLERLGF 125

Query: 124 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMML 159
            + +++    C  MI+ +D NLDG +DFEEFKNM+L
Sbjct: 126 EEEAKAW--DCGRMIRVHDKNLDGFVDFEEFKNMIL 153

[Close]

BLAST of MELO3C008466.2 vs. Swiss-Prot
Match: sp|Q9FI19|CML43_ARATH (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 6.7e-20
Identity = 60/155 (38.71%), Postives = 86/155 (55.48%), Query Frame = 0


Query: 9   LHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFL------EKPSLDFDEFLFF 68
           LHR+F   DKN DG + ++EL   L R+G+     +L+S +      +K  L FD+F   
Sbjct: 29  LHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFDDFAAL 88

Query: 69  YESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRLEL 128
           ++++ +       GG   C     E D E    AF VFD +GDGFIS  EL+ VL +L L
Sbjct: 89  HKTLDE----SFFGGEGSCCDGSPESDLE---EAFNVFDEDGDGFISAVELQKVLKKLGL 148

Query: 129 WDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
            +A   E++    MI + D+N DG++DF EFKNMM
Sbjct: 149 PEA--GEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

[Close]

BLAST of MELO3C008466.2 vs. Swiss-Prot
Match: sp|Q2QVI1|CML28_ORYSJ (Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 GN=CML28 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.8e-17
Identity = 61/168 (36.31%), Postives = 85/168 (50.60%), Query Frame = 0


Query: 4   ISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEK------PSLDFD 63
           + S +L ++FK  DKN DG +  +EL       GI +  +EL++ ++K        +D +
Sbjct: 1   MDSTELRKVFKMFDKNGDGRITKKELGESFKNFGIFIPDDELDATMDKIDANGDGCVDVE 60

Query: 64  EFLFFYESI--------SKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFIS 123
           EF   Y SI        + +      GG  G   D+ E   E    AF VFD NGDGFI+
Sbjct: 61  EFGLLYRSILGDDAAGRAPRTAAAAIGGEGGAPDDEDEGMRE----AFNVFDQNGDGFIT 120

Query: 124 CDELENVLVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
            DEL +VL  L L     +  D CR MI   D + DG++DF+EFK MM
Sbjct: 121 VDELRSVLSSLGLKHGRTA--DDCRRMISMVDADGDGRVDFKEFKQMM 162

[Close]

BLAST of MELO3C008466.2 vs. Swiss-Prot
Match: sp|P43187|ALLB3_BETPN (Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 4.1e-17
Identity = 57/164 (34.76%), Postives = 91/164 (55.49%), Query Frame = 0


Query: 9   LHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLE------KPSLDFDEFLFF 68
           L RIF   DKN DG++ + EL   L+ +G++  + ELES ++         L F++F+  
Sbjct: 41  LRRIFDLFDKNSDGIITVDELSRALNLLGLETDLSELESTVKSFTREGNIGLQFEDFISL 100

Query: 69  YESISKQNKGECKGGVAGCVQDDHEDDTEIVYMA---------FKVFDMNGDGFISCDEL 128
           ++S++     +      G  +DD+E+D     ++         FKVFD +GDG+IS  EL
Sbjct: 101 HQSLN-----DSYFAYGGEDEDDNEEDMRKSILSQEEADSFGGFKVFDEDGDGYISAREL 160

Query: 129 ENVLVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
           + VL +L    +  SE+D    MI + D+N DG++DF EFK+MM
Sbjct: 161 QMVLGKLGF--SEGSEIDRVEKMIVSVDSNRDGRVDFFEFKDMM 197

[Close]

BLAST of MELO3C008466.2 vs. Swiss-Prot
Match: sp|Q9SVG9|CML42_ARATH (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 9.1e-17
Identity = 57/157 (36.31%), Postives = 85/157 (54.14%), Query Frame = 0


Query: 9   LHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLE------KPSLDFDEFLFF 68
           L RIF   DKN DG + ++EL   L R+G+   + +L+S +E         L+FD+F   
Sbjct: 30  LQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFDDFSSL 89

Query: 69  YESI--SKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 128
           ++++  S                   E+++++   AFKVFD NGDGFIS  EL+ VL +L
Sbjct: 90  HKTLDDSXXXXXXXXXXXXXXXXXXXENESDLA-EAFKVFDENGDGFISARELQTVLKKL 149

Query: 129 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMM 158
            L +    E++    MI + D N DG++DF EFKNMM
Sbjct: 150 GLPEG--GEMERVEKMIVSVDRNQDGRVDFFEFKNMM 183

[Close]

BLAST of MELO3C008466.2 vs. TrEMBL
Match: tr|A0A1S3B3T2|A0A1S3B3T2_CUCME (probable calcium-binding protein CML44 OS=Cucumis melo OX=3656 GN=LOC103485689 PE=4 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 3.3e-88
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60
           MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120
           FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120

Query: 121 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 162
           ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

[Close]

BLAST of MELO3C008466.2 vs. TrEMBL
Match: tr|A0A0A0KFQ6|A0A0A0KFQ6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376250 PE=4 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 7.3e-80
Identity = 148/165 (89.70%), Postives = 156/165 (94.55%), Query Frame = 0


Query: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60
           M PISSKDLHRIFKKLDKNCDGL+CLQEL WLLD IGIQLTMEELESFLE+PSLDFDEFL
Sbjct: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGVAGCVQDDH----EDDTEIVYMAFKVFDMNGDGFISCDELENV 120
           FFYESISKQNKGECKGGVAGCVQD+     +DD EIVY+AFKVFDMNGDGFISCDELENV
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120

Query: 121 LVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 162
           LV+LELWDASRS+VDYCRSMI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LVKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 165

[Close]

BLAST of MELO3C008466.2 vs. TrEMBL
Match: tr|A0A061FHR5|A0A061FHR5_THECC (Calcium-binding EF-hand family protein OS=Theobroma cacao OX=3641 GN=TCM_035222 PE=4 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 7.7e-45
Identity = 100/164 (60.98%), Postives = 124/164 (75.61%), Query Frame = 0


Query: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60
           MSP+S  DL RIF+KLDKN DGL+ L+EL WLL RIG+Q ++EEL SF+ KP LDF+EFL
Sbjct: 29  MSPLSKNDLQRIFEKLDKNGDGLVSLEELNWLLVRIGVQFSLEELGSFVGKPCLDFNEFL 88

Query: 61  FFYESISKQNKGECKGGVAGCVQDD----HEDDTEIVYMAFKVFDMNGDGFISCDELENV 120
           FFY+SIS QN        AG  ++D     ++D+++V  AFKVFD+NGDGFISC+EL++V
Sbjct: 89  FFYDSISNQNDDGKNMEPAGSGEEDGLIQADEDSDLV-KAFKVFDLNGDGFISCEELQSV 148

Query: 121 LVRLELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLT 161
           LVRL LWD S  +   CR+MI  YDTNLDG +DFEEFKNMML T
Sbjct: 149 LVRLGLWDESSGK--DCRNMICFYDTNLDGMVDFEEFKNMMLHT 189

[Close]

BLAST of MELO3C008466.2 vs. TrEMBL
Match: tr|A0A0A0KLD7|A0A0A0KLD7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G173500 PE=4 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.2e-43
Identity = 94/128 (73.44%), Postives = 111/128 (86.72%), Query Frame = 0


Query: 1   MSP-ISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEF 60
           MSP ISS+DL+RIFKKLDKN DGL+CLQELKWL DR+G++LTMEELESFLEKPSLDFD+F
Sbjct: 1   MSPTISSEDLYRIFKKLDKNGDGLICLQELKWLFDRVGVELTMEELESFLEKPSLDFDQF 60

Query: 61  LFFYESISKQNKGEC-KGGVAGCV-QDDHEDDTEIVYMAFKVFDM-NGDGFISCDELENV 120
           LF Y+SISKQNKGEC +  V GC+ +DDHE+D E++ MAFKVF+M + DGFISCD LENV
Sbjct: 61  LFCYKSISKQNKGECEEEDVLGCLEEDDHEEDMEMICMAFKVFEMSDDDGFISCDGLENV 120

Query: 121 LVRLELWD 125
           L RL  +D
Sbjct: 121 LARLNEYD 128

[Close]

BLAST of MELO3C008466.2 vs. TrEMBL
Match: tr|A0A2P6PXJ9|A0A2P6PXJ9_ROSCH (Putative EF-hand domain pair protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr6g0296831 PE=4 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.2e-42
Identity = 92/161 (57.14%), Postives = 121/161 (75.16%), Query Frame = 0


Query: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60
           MSP+SS DL RIF+KLDKN DG + L+EL WLL+RIG++ +++ELES + KPSLD++EFL
Sbjct: 1   MSPLSSNDLERIFEKLDKNGDGQVSLEELNWLLERIGVEFSLQELESLVGKPSLDWNEFL 60

Query: 61  FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120
           FFY+SIS  +  +   G      +DH+D++++V  AF VFD+NGDGFISC+ELENVL RL
Sbjct: 61  FFYKSISTPDSNDGDDGHGDGSANDHDDESDLV-KAFNVFDINGDGFISCEELENVLRRL 120

Query: 121 ELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 162
            + +   +    CRSMI  YDTNLDG LDF+EFK+MML  T
Sbjct: 121 GVME--ENSCRNCRSMIHVYDTNLDGLLDFQEFKHMMLHNT 158

The following BLAST results are available for this feature:

BLAST of MELO3C008466.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008441608.1	5.0e-88	100.00	PREDICTED: probable calcium-binding protein CML44 [Cucumis melo]	[more]
XP_004149438.1	1.1e-79	89.70	PREDICTED: probable calcium-binding protein CML44 [Cucumis sativus] >KGN47684.1 ...	[more]
XP_023551499.1	4.8e-67	83.54	probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo]	[more]
XP_022939513.1	1.1e-66	82.91	probable calcium-binding protein CML44 [Cucurbita moschata]	[more]
XP_022993348.1	4.1e-66	81.99	probable calcium-binding protein CML44 [Cucurbita maxima]	[more]

BLAST of MELO3C008466.2 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G21550.1	7.5e-30	47.44	Calcium-binding EF-hand family protein	[more]
AT5G44460.1	3.7e-21	38.71	calmodulin like 43	[more]
AT4G20780.1	5.0e-18	36.31	calmodulin like 42	[more]
AT4G12860.1	1.4e-15	32.69	EF hand calcium-binding protein family	[more]
AT1G24620.1	3.1e-15	33.33	EF hand calcium-binding protein family	[more]

BLAST of MELO3C008466.2 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9LPK5\|CML44_ARATH	1.4e-28	47.44	Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 ...	[more]
sp\|Q9FI19\|CML43_ARATH	6.7e-20	38.71	Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ...	[more]
sp\|Q2QVI1\|CML28_ORYSJ	1.8e-17	36.31	Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
sp\|P43187\|ALLB3_BETPN	4.1e-17	34.76	Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1	[more]
sp\|Q9SVG9\|CML42_ARATH	9.1e-17	36.31	Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1	[more]

BLAST of MELO3C008466.2 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3B3T2\|A0A1S3B3T2_CUCME	3.3e-88	100.00	probable calcium-binding protein CML44 OS=Cucumis melo OX=3656 GN=LOC103485689 P...	[more]
tr\|A0A0A0KFQ6\|A0A0A0KFQ6_CUCSA	7.3e-80	89.70	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376250 PE=4 SV=1	[more]
tr\|A0A061FHR5\|A0A061FHR5_THECC	7.7e-45	60.98	Calcium-binding EF-hand family protein OS=Theobroma cacao OX=3641 GN=TCM_035222 ...	[more]
tr\|A0A0A0KLD7\|A0A0A0KLD7_CUCSA	4.2e-43	73.44	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G173500 PE=4 SV=1	[more]
tr\|A0A2P6PXJ9\|A0A2P6PXJ9_ROSCH	1.2e-42	57.14	Putative EF-hand domain pair protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr6...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS
IPR002048	EF_hand_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0046872	metal ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C008466.2.1	MELO3C008466.2.1	mRNA

InterPro

Analysis Name: InterPro Annotations of melon v3.6.1
Date Performed: 2018-09-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 8..36 e-value: 0.011 score: 24.9 coord: 132..160 e-value: 0.01 score: 25.0 coord: 92..120 e-value: 0.037 score: 23.2
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 94..157 e-value: 2.2E-9 score: 37.6
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 12..30 e-value: 0.0024 score: 17.2
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 128..161 score: 13.593
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 4..39 score: 11.863
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 88..123 score: 12.365
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 8..60 e-value: 2.81107E-7 score: 43.3053
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 96..157 e-value: 8.30158E-12 score: 56.0169
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 1..64 e-value: 4.5E-9 score: 38.2
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 65..157 e-value: 9.6E-19 score: 69.5
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 1..160
None	No IPR available	PANTHER	PTHR10891:SF530	CALCIUM-BINDING PROTEIN CML44-RELATED	coord: 1..160
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 17..29
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 101..113
IPR011992	EF-hand domain pair	SUPERFAMILY	SSF47473	EF-hand	coord: 5..158

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
MELO3C008466.2	Csa6G376250	Cucumber (Chinese Long) v2	cumedB449
MELO3C008466.2	CSPI06G19980	Wild cucumber (PI 183967)	cpimedB458
MELO3C008466.2	Cucsa.328100	Cucumber (Gy14) v1	cgymedB540
MELO3C008466.2	CmaCh15G006470	Cucurbita maxima (Rimu)	cmamedB307
MELO3C008466.2	CmaCh04G023940	Cucurbita maxima (Rimu)	cmamedB763
MELO3C008466.2	CmoCh04G025100	Cucurbita moschata (Rifu)	cmomedB760
MELO3C008466.2	CmoCh15G006570	Cucurbita moschata (Rifu)	cmomedB293
MELO3C008466.2	Lsi08G004690	Bottle gourd (USVL1VR-Ls)	lsimedB494
MELO3C008466.2	Cp4.1LG01g20760	Cucurbita pepo (Zucchini)	cpemedB466
MELO3C008466.2	Cp4.1LG13g04330	Cucurbita pepo (Zucchini)	cpemedB200
MELO3C008466.2	CsGy6G019460	Cucumber (Gy14) v2	cgybmedB407
MELO3C008466.2	Cla013901	Watermelon (97103) v1	medwmB301
MELO3C008466.2	ClCG08G006060	Watermelon (Charleston Gray)	medwcgB293
MELO3C008466.2	Cla97C08G148820	Watermelon (97103) v2	medwmbB294
MELO3C008466.2	Bhi04G001477	Wax gourd	medwgoB324
MELO3C008466.2	Carg01562	Silver-seed gourd	carmedB0835
MELO3C008466.2	Carg15752	Silver-seed gourd	carmedB0066
MELO3C008466.2	CsaV3_6G034010	Cucumber (Chinese Long) v3	cucmedB467

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None