Cucsat.G15307 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G15307
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Cytochrome P450
Location	ctg2009: 550454 .. 551259 (-)
RNA-Seq Expression	Cucsat.G15307
Synteny	Cucsat.G15307

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTATGTTTATATATATAGTTAGTTTTTAAAGTTTGGTATTTTTTATTCTTATTTATCTTGAGATAGAGATTAGGAAATTGATTTTAATCATTGGTGTGCAAGTATATTTATTTGAAGCTGAAAGCATGCTATGAAGCAATACGATTTTCTCGTTTTCCAAAAATCAAAACTATGATAATTTACCTAACAATCTTTTTCTGTCTTCTAATCCCCATCTTCCTCTTCCTTCGAAGATCATCCAAAAAGCGGCAGCTTCCTCCAGGTTCGCTAGGGTTTCCATTGATCGGTCAGAGTCTCAGCCTCCTCCGCGCCATGCGAACCAACACCGCTGAACAATGGGCGCAGCAGAGGATCAGAAACTACGGGGCCATCTCCAAGCTAACCCTATTCGGAAAGCCAACTGTGTTCATCGGTGGGCAGAGCGGGAACAAGTTGATTTTCAGCGGGGATTGCGCAGTAGTTTCGAATCAGCAAAATGAGTCTCTTAGAGCAATTTTAGGGGAAAGGAATTTGTTGGAGCTGATTGGGGAAGATCATAAGCGTGTGAGAAATGCGTTGGTTTCGTTCTTGAAGCCGGAATGTTTGAAGGAATATGTGGGCAAAATGGATGAAGAAATCAGATCACATATCCGCATGCATTGGCACTGTAAACAAGAGGTTACTGTGAGTACACCTTTTCCTACTTTTTCTTTTTTCTTACGTTTTTCAAAATTGAAAATTGGTATTTCAATTTTCATTTCTGAGTTCTCAACAGATATACACATTTCTACTATTGTTTGAAAAAATAATAATAAAGAACCCCGTTGCCGGGGATAAAAAACGGTAATACCCACTGCCACACTACAACGACTTAATTTGTTACATTAACTAAATAAAGAATATATAAAACGAGTTTAAAAAAAAAAAAAAATTGAGAGTTGAATGTGTTATTATAATTATGATGTTTTGTCTATTGAGATGATGTTTATTATGCACATAGGTATTGCCATTGATGAAGACTCTCACCTTCAACATTGTATGTTCTCTTCTGTTTGGTCTTGAGCAAGGTACGAGAAGAGAAAGAATGATAGAATGCTTTCGAGTAATGATAAGCGGAGTATGGTCGATCCCGATAAACTTGCCGTTCACAAGGTACAACCAAAGCCGGCGTGCGAGTAGAAAAATTCAAGAGATGCTGAAGGAATTGTTGGATGAAAAGAGAGTTGAATTAGAAGAAAAAGGAGGCTCCTCACACCAAGATCTAATTACTTGTTTGTTAAGCATAAGGAATGAAGAAAATGAACTAGTATTGAGTGAAAAGGAAATTGTGCACAATATTATGTTGGTATTGGTTGCTGGATTTGATACTTCATCGGTTCTCATTACGTTCATGATGCGAAATTTAGCTAATAATCCAACTGTTTATGCAGCTGTTCTTCAAGGTATGCTGCTTCCAATTATCTTATACACTCTTTATATTACAATGACTGTATATATAATCATATCTTTCTTCAAGCTTATTTGTTTTTTGTTGTGGTGTGTGTGTGTGTGTGGTTAATTACAGAACAAGAAGAGATAGCAAGAAGTAAAAAATGTGGAGAGTTGCTTAATTGGGAAGATCTTGCCAAGATGAAGTACACATGGAGAGTTGCATTAGAGACCCTAAGGTTGGTGGCACCTATATTTGGAGGCTTCAGAAAGGCTATGAATGATATTCAATTTGGTGGCTACCTTATTCCTAAAGGATGGCAAGTAAGTCAAACTTTCTTCCTTTTTTGACTTGAGGATCTCAAGTGAAACTTTATGTAACATCTAATAAACGTATTCATCCAACCACACTATTGGAATTCAGATATTTTGGACATCACCAGTTACTCATTTGGATGACACCATATTTAGAGAACCATCAAAATTCGACCCAGATAGGTTCAAAAATCCAGCGTCCATTCCACCGTATTGCTTCCTTGGATTTGGCAGCGGTCCGAGAATTTGTCCAGGCAATGAGTTTGCTAGGGTTGAGACTCTTGTTACCATACATTACTTGATCACCCAATTCACATGGAAACTACTTTTGGATGACCATTTTATCAGAGATCCAATGCCAACACCTACCAAAGGAATGCCTATCAAAATTTGGCCCAGATCACAATCTTTCATGGCAGTGTAGAATGCGTAGAAATGAGAATCATGGTTAGTGACATATTTGGGAGTGAGAAGATCTCAAAAATTGTTAAAATCACCTGTCTTGTTTAAAATCATTCGGATAGTATGTAAAAAATAAGTTCGGGCGTAAAATCAAACATTAAATTATTGAGAAATGTGTTTTTTCAACTGATTTTGATAATGACAAAAGTTAATTCAACAAAGGATAGTTCTAGCAATTTTTCTATGCTTGAATAACGATACATTGCAATTCAACTTAACTCACAACCAATTAGTCCTAGAAACTAATTGTCCTCAAGCAATGAATCAAGAAAGAAATCAAAATTCATACTAATTAAAGAAAATGCTTTAAGGAAGAAATCAAAATTCATACTAATTAAAGAACATGCTTTAAGGAAGAAATCTAAATTCATACTAATTAAAGAAAATGCTTTCATCTTAGAATTGCATTAAGATCCAAAGCTTCTATGTTGAGACTCTCAAGAGCAAGGAATTGAAATTTAAATCGGGCATTGAAGAAAAATCAGTAGTATAGACATGCTAAAGATTAATACAAAGATTCACAATCCAATCATGCCCCAAAAAATGTGCTTAAGCATCGCTAGAAATTAAAATTAA

Coding sequence (CDS)

ATGATAATTTACCTAACAATCTTTTTCTGTCTTCTAATCCCCATCTTCCTCTTCCTTCGAAGATCATCCAAAAAGCGGCAGCTTCCTCCAGGTTCGCTAGGGTTTCCATTGATCGGTCAGAGTCTCAGCCTCCTCCGCGCCATGCGAACCAACACCGCTGAACAATGGGCGCAGCAGAGGATCAGAAACTACGGGGCCATCTCCAAGCTAACCCTATTCGGAAAGCCAACTGTGTTCATCGGTGGGCAGAGCGGGAACAAGTTGATTTTCAGCGGGGATTGCGCAGTAGTTTCGAATCAGCAAAATGAGTCTCTTAGAGCAATTTTAGGGGAAAGGAATTTGTTGGAGCTGATTGGGGAAGATCATAAGCGTGTGAGAAATGCGTTGGTTTCGTTCTTGAAGCCGGAATGTTTGAAGGAATATGTGGGCAAAATGGATGAAGAAATCAGATCACATATCCGCATGCATTGGCACTGTAAACAAGAGGTTACTGTATTGCCATTGATGAAGACTCTCACCTTCAACATTGTATGTTCTCTTCTGTTTGGTCTTGAGCAAGGTACGAGAAGAGAAAGAATGATAGAATGCTTTCGAGTAATGATAAGCGGAGTATGGTCGATCCCGATAAACTTGCCGTTCACAAGGTACAACCAAAGCCGGCGTGCGAGTAGAAAAATTCAAGAGATGCTGAAGGAATTGTTGGATGAAAAGAGAGTTGAATTAGAAGAAAAAGGAGGCTCCTCACACCAAGATCTAATTACTTGTTTGTTAAGCATAAGGAATGAAGAAAATGAACTAGTATTGAGTGAAAAGGAAATTGTGCACAATATTATGTTGGTATTGGTTGCTGGATTTGATACTTCATCGGTTCTCATTACGTTCATGATGCGAAATTTAGCTAATAATCCAACTGTTTATGCAGCTGTTCTTCAAGAACAAGAAGAGATAGCAAGAAGTAAAAAATGTGGAGAGTTGCTTAATTGGGAAGATCTTGCCAAGATGAAGTACACATGGAGAGTTGCATTAGAGACCCTAAGGTTGGTGGCACCTATATTTGGAGGCTTCAGAAAGGCTATGAATGATATTCAATTTGGTGGCTACCTTATTCCTAAAGGATGGCAAGTAAGTCAAACTTTCTTCCTTTTTTGA

Protein sequence

MIIYLTIFFCLLIPIFLFLRRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQRIRNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGEDHKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSLLFGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVELEEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYLIPKGWQVSQTFFLF

Homology

[Close]

BLAST of Cucsat.G15307 vs. ExPASy Swiss-Prot
Match: A0A0S2II38 (Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=1 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 4.2e-50
Identity = 83/160 (51.88%), Postives = 118/160 (73.75%), Query Frame = 0


Query: 14  EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYLIPK 73
           EQ EIA+SKK GELLNWED+ KMKY+W VA E LRL  P+ G FR+A+ D  + G+ IPK
Sbjct: 320 EQMEIAKSKKAGELLNWEDIQKMKYSWNVACEVLRLAPPLQGAFREALTDFSYNGFSIPK 379

Query: 74  GWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFARVET 133
           GW+++W++  TH +  +F EP KFDP RF+  A  PPY F+ FG GPR+CPG E+AR+E 
Sbjct: 380 GWKLYWSANSTHRNSEVFPEPLKFDPSRFEG-AGPPPYSFVPFGGGPRMCPGKEYARLEI 439

Query: 134 LVTIHYLITQFTW-KLLLDDHFIRDPMPTPTKGMPIKIWP 173
           LV +H+++ +F W K++ D+  + +PMP P KG+P++++P
Sbjct: 440 LVFMHHVVKRFKWEKVIPDEKIVVNPMPIPAKGLPVRLFP 478

[Close]

BLAST of Cucsat.G15307 vs. ExPASy Swiss-Prot
Match: I7C6E8 (Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 2.3e-48
Identity = 80/160 (50.00%), Postives = 117/160 (73.12%), Query Frame = 0


Query: 14  EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYLIPK 73
           EQ EI +SKK GELL WED+ KMKY+W VA E LRL  P+ G FR+A++D  + G+ IPK
Sbjct: 320 EQMEIVKSKKAGELLKWEDIQKMKYSWNVACEVLRLAPPLQGAFREALSDFTYNGFSIPK 379

Query: 74  GWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFARVET 133
           GW+++W++  TH++  +F EP KFDP RF + A  PP+ F+ FG GPR+CPG E+AR+E 
Sbjct: 380 GWKLYWSANSTHINSEVFPEPLKFDPSRF-DGAGPPPFSFVPFGGGPRMCPGKEYARLEI 439

Query: 134 LVTIHYLITQFTW-KLLLDDHFIRDPMPTPTKGMPIKIWP 173
           LV +H+L+ +F W K++ D+  + +PMP P  G+P++++P
Sbjct: 440 LVFMHHLVKRFKWEKVIPDEKIVVNPMPIPANGLPVRLFP 478

[Close]

BLAST of Cucsat.G15307 vs. ExPASy Swiss-Prot
Match: K4CEE8 (Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A46 PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 4.3e-47
Identity = 81/160 (50.62%), Postives = 116/160 (72.50%), Query Frame = 0


Query: 14  EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYLIPK 73
           EQ EIA+SK  GELL+WED+ KMKY+W VA E LRL  P+ G FR+A+ D  F G+ IPK
Sbjct: 319 EQIEIAKSKGPGELLSWEDIKKMKYSWNVACEVLRLAPPLQGAFREALVDFTFNGFSIPK 378

Query: 74  GWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFARVET 133
           GW+I+W++  TH++  +F +P KFDP RF+      PY F+ FG GPR+CPG E+AR+E 
Sbjct: 379 GWKIYWSANSTHINPEVFVDPLKFDPSRFEGNGP-APYTFVPFGGGPRMCPGKEYARLEI 438

Query: 134 LVTIHYLITQFTW-KLLLDDHFIRDPMPTPTKGMPIKIWP 173
           LV +H+L+ +F+W K++ D+  + +PMP P  G+PI+++P
Sbjct: 439 LVFMHHLVKRFSWKKIIRDEKIVVNPMPIPANGLPIRLYP 477

[Close]

BLAST of Cucsat.G15307 vs. ExPASy Swiss-Prot
Match: A5BFI4 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2)

HSP 1 Score: 186.4 bits (472), Expect = 2.8e-46
Identity = 82/160 (51.25%), Postives = 111/160 (69.38%), Query Frame = 0


Query: 14  EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYLIPK 73
           EQ EIA SK  GELLNW+D+ KM+Y+W VA E +RL  P+ G FR+A+ D  F G+ IPK
Sbjct: 319 EQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQGAFREAITDFVFNGFSIPK 378

Query: 74  GWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFARVET 133
           GW+++W++  TH     F +P KFDP RF+  A   PY F+ FG GPR+CPG E+AR+E 
Sbjct: 379 GWKLYWSANSTHKSLECFPQPEKFDPTRFEG-AGPAPYTFVPFGGGPRMCPGKEYARLEI 438

Query: 134 LVTIHYLITQFTW-KLLLDDHFIRDPMPTPTKGMPIKIWP 173
           L+ +H L+ +F W KLL D+  I DPMP P KG+P+++ P
Sbjct: 439 LIFMHNLVKRFKWDKLLPDEKIIVDPMPMPAKGLPVRLHP 477

[Close]

BLAST of Cucsat.G15307 vs. ExPASy Swiss-Prot
Match: Q2MJ20 (Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 3.6e-46
Identity = 82/160 (51.25%), Postives = 111/160 (69.38%), Query Frame = 0


Query: 14  EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYLIPK 73
           EQ EIA+SK  GELLNW+DL KMKY+W VA E +RL  P+ GGFR+A+ D  F G+ IPK
Sbjct: 318 EQMEIAKSKPAGELLNWDDLKKMKYSWNVACEVMRLSPPLQGGFREAITDFMFNGFSIPK 377

Query: 74  GWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFARVET 133
           GW+++W++  TH +   F  P KFDP RF+      PY F+ FG GPR+CPG E+AR+E 
Sbjct: 378 GWKLYWSANSTHKNAECFPMPEKFDPTRFEGNGP-APYTFVPFGGGPRMCPGKEYARLEI 437

Query: 134 LVTIHYLITQFTW-KLLLDDHFIRDPMPTPTKGMPIKIWP 173
           LV +H L+ +F W K++ D+  I DP P P K +PI+++P
Sbjct: 438 LVFMHNLVKRFKWEKVIPDEKIIVDPFPIPAKDLPIRLYP 476

[Close]

BLAST of Cucsat.G15307 vs. NCBI nr
Match: XP_004140824.1 (cytochrome P450 716B1 [Cucumis sativus] >KGN57442.1 hypothetical protein Csa_010272 [Cucumis sativus])

HSP 1 Score: 365 bits (937), Expect = 5.20e-123
Identity = 167/170 (98.24%), Postives = 169/170 (99.41%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL
Sbjct: 309 VLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 368

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 130
           IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR
Sbjct: 369 IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 428

Query: 131 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV 180
           VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV
Sbjct: 429 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV 478

[Close]

BLAST of Cucsat.G15307 vs. NCBI nr
Match: XP_008457111.1 (PREDICTED: cytochrome P450 716B1-like [Cucumis melo] >KAA0047356.1 cytochrome P450 716B1-like protein [Cucumis melo var. makuwa] >TYK14034.1 cytochrome P450 716B1-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 351 bits (901), Expect = 1.88e-117
Identity = 161/169 (95.27%), Postives = 165/169 (97.63%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL
Sbjct: 309 VLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 368

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 130
           IPKGWQIFWTSPVTHLDD IFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR
Sbjct: 369 IPKGWQIFWTSPVTHLDDNIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 428

Query: 131 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMA 179
           VETLVTIHYLITQFTWKL+ DDHFIRDPMPTPTKGMPIKIWPR Q+FMA
Sbjct: 429 VETLVTIHYLITQFTWKLISDDHFIRDPMPTPTKGMPIKIWPR-QTFMA 476

[Close]

BLAST of Cucsat.G15307 vs. NCBI nr
Match: XP_038899130.1 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Benincasa hispida])

HSP 1 Score: 322 bits (824), Expect = 7.56e-106
Identity = 149/174 (85.63%), Postives = 155/174 (89.08%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ EQEEIARSKKCGELL WEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDI+FGGYL
Sbjct: 308 VLQEQEEIARSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIEFGGYL 367

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPG----- 130
           IPKGWQIFW SPVTHLD TIFREP KFDP+RF NP SIPPYCF+GFG GPRICPG     
Sbjct: 368 IPKGWQIFWASPVTHLDGTIFREPLKFDPNRFDNPTSIPPYCFVGFGGGPRICPGGPRIC 427

Query: 131 --NEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSF 177
             NEFARVETLVTIHYLIT+FTWKLLLDDHFIRDPMPTPTKGMPIKI PR  S 
Sbjct: 428 PGNEFARVETLVTIHYLITRFTWKLLLDDHFIRDPMPTPTKGMPIKICPRETSM 481

[Close]

BLAST of Cucsat.G15307 vs. NCBI nr
Match: XP_022139355.1 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Momordica charantia])

HSP 1 Score: 308 bits (789), Expect = 1.26e-100
Identity = 138/167 (82.63%), Postives = 150/167 (89.82%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ EQEEIARSK+CGELL WEDLAKMKYTWRVALETLRLVAP+FGGFRKAM DI+FGGYL
Sbjct: 310 VLQEQEEIARSKECGELLTWEDLAKMKYTWRVALETLRLVAPVFGGFRKAMKDIEFGGYL 369

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 130
           IPKGWQIFW SP+THLDDTIF EP KFDP+RF+N AS+P YCF+ FG GPRICPG EFAR
Sbjct: 370 IPKGWQIFWASPMTHLDDTIFEEPLKFDPNRFENQASVPXYCFVAFGGGPRICPGYEFAR 429

Query: 131 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSF 177
           VETLVTIHYLITQFTWKLLLDD FIRDPMP PTKG+PIK+ PR  S 
Sbjct: 430 VETLVTIHYLITQFTWKLLLDDLFIRDPMPVPTKGLPIKVCPRETSM 476

[Close]

BLAST of Cucsat.G15307 vs. NCBI nr
Match: KAE8125584.1 (hypothetical protein FH972_020367 [Carpinus fangiana])

HSP 1 Score: 267 bits (683), Expect = 9.80e-88
Identity = 115/163 (70.55%), Postives = 140/163 (85.89%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ E EEIA++K  GE L WEDL KMKYTWRVA+ETLR+V P+FGGFRKA  DI++GGYL
Sbjct: 80  VLQEHEEIAKTKLSGEYLTWEDLTKMKYTWRVAMETLRMVPPVFGGFRKAQKDIEYGGYL 139

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 130
           IPKGWQIFWT+P+TH+D++IF+EPSKF P RF++ ASIPPY F+ FG GPRICPG EFAR
Sbjct: 140 IPKGWQIFWTTPMTHMDNSIFQEPSKFYPSRFESQASIPPYSFVAFGGGPRICPGYEFAR 199

Query: 131 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPR 173
           +ETLVTIHYL+TQFTWKL  D+ F RDP+P PT+G+PIK+ PR
Sbjct: 200 IETLVTIHYLVTQFTWKLCSDNTFSRDPIPIPTQGLPIKLVPR 242

[Close]

BLAST of Cucsat.G15307 vs. ExPASy TrEMBL
Match: A0A0A0LA02 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G186690 PE=3 SV=1)

HSP 1 Score: 365 bits (937), Expect = 2.52e-123
Identity = 167/170 (98.24%), Postives = 169/170 (99.41%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL
Sbjct: 309 VLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 368

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 130
           IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR
Sbjct: 369 IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 428

Query: 131 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV 180
           VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV
Sbjct: 429 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV 478

[Close]

BLAST of Cucsat.G15307 vs. ExPASy TrEMBL
Match: A0A5A7TZG7 (Cytochrome P450 716B1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold363G00020 PE=3 SV=1)

HSP 1 Score: 351 bits (901), Expect = 9.11e-118
Identity = 161/169 (95.27%), Postives = 165/169 (97.63%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL
Sbjct: 309 VLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 368

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 130
           IPKGWQIFWTSPVTHLDD IFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR
Sbjct: 369 IPKGWQIFWTSPVTHLDDNIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 428

Query: 131 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMA 179
           VETLVTIHYLITQFTWKL+ DDHFIRDPMPTPTKGMPIKIWPR Q+FMA
Sbjct: 429 VETLVTIHYLITQFTWKLISDDHFIRDPMPTPTKGMPIKIWPR-QTFMA 476

[Close]

BLAST of Cucsat.G15307 vs. ExPASy TrEMBL
Match: A0A1S3C4V0 (cytochrome P450 716B1-like OS=Cucumis melo OX=3656 GN=LOC103496862 PE=3 SV=1)

HSP 1 Score: 351 bits (901), Expect = 9.11e-118
Identity = 161/169 (95.27%), Postives = 165/169 (97.63%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL
Sbjct: 309 VLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 368

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 130
           IPKGWQIFWTSPVTHLDD IFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR
Sbjct: 369 IPKGWQIFWTSPVTHLDDNIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 428

Query: 131 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMA 179
           VETLVTIHYLITQFTWKL+ DDHFIRDPMPTPTKGMPIKIWPR Q+FMA
Sbjct: 429 VETLVTIHYLITQFTWKLISDDHFIRDPMPTPTKGMPIKIWPR-QTFMA 476

[Close]

BLAST of Cucsat.G15307 vs. ExPASy TrEMBL
Match: A0A6J1CC35 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like OS=Momordica charantia OX=3673 GN=LOC111010306 PE=3 SV=1)

HSP 1 Score: 308 bits (789), Expect = 6.10e-101
Identity = 138/167 (82.63%), Postives = 150/167 (89.82%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ EQEEIARSK+CGELL WEDLAKMKYTWRVALETLRLVAP+FGGFRKAM DI+FGGYL
Sbjct: 310 VLQEQEEIARSKECGELLTWEDLAKMKYTWRVALETLRLVAPVFGGFRKAMKDIEFGGYL 369

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 130
           IPKGWQIFW SP+THLDDTIF EP KFDP+RF+N AS+P YCF+ FG GPRICPG EFAR
Sbjct: 370 IPKGWQIFWASPMTHLDDTIFEEPLKFDPNRFENQASVPXYCFVAFGGGPRICPGYEFAR 429

Query: 131 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSF 177
           VETLVTIHYLITQFTWKLLLDD FIRDPMP PTKG+PIK+ PR  S 
Sbjct: 430 VETLVTIHYLITQFTWKLLLDDLFIRDPMPVPTKGLPIKVCPRETSM 476

[Close]

BLAST of Cucsat.G15307 vs. ExPASy TrEMBL
Match: A0A5N6RXE7 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_020367 PE=3 SV=1)

HSP 1 Score: 267 bits (683), Expect = 4.75e-88
Identity = 115/163 (70.55%), Postives = 140/163 (85.89%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ E EEIA++K  GE L WEDL KMKYTWRVA+ETLR+V P+FGGFRKA  DI++GGYL
Sbjct: 80  VLQEHEEIAKTKLSGEYLTWEDLTKMKYTWRVAMETLRMVPPVFGGFRKAQKDIEYGGYL 139

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 130
           IPKGWQIFWT+P+TH+D++IF+EPSKF P RF++ ASIPPY F+ FG GPRICPG EFAR
Sbjct: 140 IPKGWQIFWTTPMTHMDNSIFQEPSKFYPSRFESQASIPPYSFVAFGGGPRICPGYEFAR 199

Query: 131 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPR 173
           +ETLVTIHYL+TQFTWKL  D+ F RDP+P PT+G+PIK+ PR
Sbjct: 200 IETLVTIHYLVTQFTWKLCSDNTFSRDPIPIPTQGLPIKLVPR 242

The following BLAST results are available for this feature:

BLAST of Cucsat.G15307 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A0S2II38	4.2e-50	51.88	Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=...	[more]
I7C6E8	2.3e-48	50.00	Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1	[more]
K4CEE8	4.3e-47	50.63	Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A46 PE=1 S...	[more]
A5BFI4	2.8e-46	51.25	Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2	[more]
Q2MJ20	3.6e-46	51.25	Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV...	[more]

BLAST of Cucsat.G15307 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140824.1	5.20e-123	98.24	cytochrome P450 716B1 [Cucumis sativus] >KGN57442.1 hypothetical protein Csa_010...	[more]
XP_008457111.1	1.88e-117	95.27	PREDICTED: cytochrome P450 716B1-like [Cucumis melo] >KAA0047356.1 cytochrome P4...	[more]
XP_038899130.1	7.56e-106	85.63	LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Benincasa hispida]	[more]
XP_022139355.1	1.26e-100	82.63	LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Momordica charantia]	[more]
KAE8125584.1	9.80e-88	70.55	hypothetical protein FH972_020367 [Carpinus fangiana]	[more]

BLAST of Cucsat.G15307 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LA02	2.52e-123	98.24	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G186690 PE=3 SV=1	[more]
A0A5A7TZG7	9.11e-118	95.27	Cytochrome P450 716B1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3C4V0	9.11e-118	95.27	cytochrome P450 716B1-like OS=Cucumis melo OX=3656 GN=LOC103496862 PE=3 SV=1	[more]
A0A6J1CC35	6.10e-101	82.63	LOW QUALITY PROTEIN: cytochrome P450 716B1-like OS=Momordica charantia OX=3673 G...	[more]
A0A5N6RXE7	4.75e-88	70.55	Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_020367 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 24..44
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 4..279
None	No IPR available	PANTHER	PTHR24286:SF237	OS07G0520300 PROTEIN	coord: 4..279
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 100..126 score: 18.81 coord: 186..210 score: 28.21 coord: 229..252 score: 27.13 coord: 80..97 score: 26.65 coord: 219..229 score: 50.64
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 147..158 score: 34.88 coord: 229..240 score: 38.15 coord: 220..229 score: 54.7 coord: 91..108 score: 30.91
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 24..255 e-value: 5.1E-56 score: 190.3
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..277 e-value: 1.1E-76 score: 260.5
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 3..279
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 222..231

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G15307.T2	Cucsat.G15307.T2	mRNA
Cucsat.G15307.T1	Cucsat.G15307.T1	mRNA
Cucsat.G15307.T5	Cucsat.G15307.T5	mRNA
Cucsat.G15307.T3	Cucsat.G15307.T3	mRNA
Cucsat.G15307.T6	Cucsat.G15307.T6	mRNA
Cucsat.G15307.T4	Cucsat.G15307.T4	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen