Cucsat.G15307.T4 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G15307.T4
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Cytochrome P450
Location	ctg2009: 550454 .. 551259 (-)
RNA-Seq Expression	Cucsat.G15307.T4
Synteny	Cucsat.G15307.T4

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTTTGTTGTGGTGTGTGTGTGTGTGTGGTTAATTACAGAACAAGAAGAGATAGCAAGAAGTAAAAAATGTGGAGAGTTGCTTAATTGGGAAGATCTTGCCAAGATGAAGTACACATGGAGAGTTGCATTAGAGACCCTAAGGTTGGTGGCACCTATATTTGGAGGCTTCAGAAAGGCTATGAATGATATTCAATTTGGTGGCTACCTTATTCCTAAAGGATGGCAAGTAAGTCAAACTTTCTTCCTTTTTTGACTTGAGGATCTCAAGTGAAACTTTATGTAACATCTAATAAACGTATTCATCCAACCACACTATTGGAATTCAGATATTTTGGACATCACCAGTTACTCATTTGGATGACACCATATTTAGAGAACCATCAAAATTCGACCCAGATAGGTTCAAAAATCCAGCGTCCATTCCACCGTATTGCTTCCTTGGATTTGGCAGCGGTCCGAGAATTTGTCCAGGCAATGAGTTTGCTAGGGTTGAGACTCTTGTTACCATACATTACTTGATCACCCAATTCACATGGAAACTACTTTTGGATGACCATTTTATCAGAGATCCAATGCCAACACCTACCAAAGGAATGCCTATCAAAATTTGGCCCAGATCACAATCTTTCATGGCAGTGTAGAATGCGTAGAAATGAGAATCATGGTTAGTGACATATTTGGGAGTGAGAAGATCTCAAAAATTGTTAAAATCACCTGTCTTGTTTAAAATCATTCGGATAGTATGTAAAAAATAAGTTCGGGCGTAAAATCAAACATTAAATTATTGAGAAATGTGTTTTTTCA

Coding sequence (CDS)

TTTTTTGTTGTGGTGTGTGTGTGTGTGTGGTTAATTACAGAACAAGAAGAGATAGCAAGAAGTAAAAAATGTGGAGAGTTGCTTAATTGGGAAGATCTTGCCAAGATGAAGTACACATGGAGAGTTGCATTAGAGACCCTAAGGTTGGTGGCACCTATATTTGGAGGCTTCAGAAAGGCTATGAATGATATTCAATTTGGTGGCTACCTTATTCCTAAAGGATGGCAAATATTTTGGACATCACCAGTTACTCATTTGGATGACACCATATTTAGAGAACCATCAAAATTCGACCCAGATAGGTTCAAAAATCCAGCGTCCATTCCACCGTATTGCTTCCTTGGATTTGGCAGCGGTCCGAGAATTTGTCCAGGCAATGAGTTTGCTAGGGTTGAGACTCTTGTTACCATACATTACTTGATCACCCAATTCACATGGAAACTACTTTTGGATGACCATTTTATCAGAGATCCAATGCCAACACCTACCAAAGGAATGCCTATCAAAATTTGGCCCAGATCACAATCTTTCATGGCAGTGTAG

Protein sequence

FFVVVCVCVWLITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV

Homology

[Close]

BLAST of Cucsat.G15307.T4 vs. ExPASy Swiss-Prot
Match: A0A0S2II38 (Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=1 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 4.2e-50
Identity = 83/160 (51.88%), Postives = 118/160 (73.75%), Query Frame = 0


Query: 14  EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYLIPK 73
           EQ EIA+SKK GELLNWED+ KMKY+W VA E LRL  P+ G FR+A+ D  + G+ IPK
Sbjct: 320 EQMEIAKSKKAGELLNWEDIQKMKYSWNVACEVLRLAPPLQGAFREALTDFSYNGFSIPK 379

Query: 74  GWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFARVET 133
           GW+++W++  TH +  +F EP KFDP RF+  A  PPY F+ FG GPR+CPG E+AR+E 
Sbjct: 380 GWKLYWSANSTHRNSEVFPEPLKFDPSRFEG-AGPPPYSFVPFGGGPRMCPGKEYARLEI 439

Query: 134 LVTIHYLITQFTW-KLLLDDHFIRDPMPTPTKGMPIKIWP 173
           LV +H+++ +F W K++ D+  + +PMP P KG+P++++P
Sbjct: 440 LVFMHHVVKRFKWEKVIPDEKIVVNPMPIPAKGLPVRLFP 478

[Close]

BLAST of Cucsat.G15307.T4 vs. ExPASy Swiss-Prot
Match: I7C6E8 (Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 2.3e-48
Identity = 80/160 (50.00%), Postives = 117/160 (73.12%), Query Frame = 0


Query: 14  EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYLIPK 73
           EQ EI +SKK GELL WED+ KMKY+W VA E LRL  P+ G FR+A++D  + G+ IPK
Sbjct: 320 EQMEIVKSKKAGELLKWEDIQKMKYSWNVACEVLRLAPPLQGAFREALSDFTYNGFSIPK 379

Query: 74  GWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFARVET 133
           GW+++W++  TH++  +F EP KFDP RF + A  PP+ F+ FG GPR+CPG E+AR+E 
Sbjct: 380 GWKLYWSANSTHINSEVFPEPLKFDPSRF-DGAGPPPFSFVPFGGGPRMCPGKEYARLEI 439

Query: 134 LVTIHYLITQFTW-KLLLDDHFIRDPMPTPTKGMPIKIWP 173
           LV +H+L+ +F W K++ D+  + +PMP P  G+P++++P
Sbjct: 440 LVFMHHLVKRFKWEKVIPDEKIVVNPMPIPANGLPVRLFP 478

[Close]

BLAST of Cucsat.G15307.T4 vs. ExPASy Swiss-Prot
Match: K4CEE8 (Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A46 PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 4.3e-47
Identity = 81/160 (50.62%), Postives = 116/160 (72.50%), Query Frame = 0


Query: 14  EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYLIPK 73
           EQ EIA+SK  GELL+WED+ KMKY+W VA E LRL  P+ G FR+A+ D  F G+ IPK
Sbjct: 319 EQIEIAKSKGPGELLSWEDIKKMKYSWNVACEVLRLAPPLQGAFREALVDFTFNGFSIPK 378

Query: 74  GWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFARVET 133
           GW+I+W++  TH++  +F +P KFDP RF+      PY F+ FG GPR+CPG E+AR+E 
Sbjct: 379 GWKIYWSANSTHINPEVFVDPLKFDPSRFEGNGP-APYTFVPFGGGPRMCPGKEYARLEI 438

Query: 134 LVTIHYLITQFTW-KLLLDDHFIRDPMPTPTKGMPIKIWP 173
           LV +H+L+ +F+W K++ D+  + +PMP P  G+PI+++P
Sbjct: 439 LVFMHHLVKRFSWKKIIRDEKIVVNPMPIPANGLPIRLYP 477

[Close]

BLAST of Cucsat.G15307.T4 vs. ExPASy Swiss-Prot
Match: A5BFI4 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2)

HSP 1 Score: 186.4 bits (472), Expect = 2.8e-46
Identity = 82/160 (51.25%), Postives = 111/160 (69.38%), Query Frame = 0


Query: 14  EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYLIPK 73
           EQ EIA SK  GELLNW+D+ KM+Y+W VA E +RL  P+ G FR+A+ D  F G+ IPK
Sbjct: 319 EQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQGAFREAITDFVFNGFSIPK 378

Query: 74  GWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFARVET 133
           GW+++W++  TH     F +P KFDP RF+  A   PY F+ FG GPR+CPG E+AR+E 
Sbjct: 379 GWKLYWSANSTHKSLECFPQPEKFDPTRFEG-AGPAPYTFVPFGGGPRMCPGKEYARLEI 438

Query: 134 LVTIHYLITQFTW-KLLLDDHFIRDPMPTPTKGMPIKIWP 173
           L+ +H L+ +F W KLL D+  I DPMP P KG+P+++ P
Sbjct: 439 LIFMHNLVKRFKWDKLLPDEKIIVDPMPMPAKGLPVRLHP 477

[Close]

BLAST of Cucsat.G15307.T4 vs. ExPASy Swiss-Prot
Match: Q2MJ20 (Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 3.6e-46
Identity = 82/160 (51.25%), Postives = 111/160 (69.38%), Query Frame = 0


Query: 14  EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYLIPK 73
           EQ EIA+SK  GELLNW+DL KMKY+W VA E +RL  P+ GGFR+A+ D  F G+ IPK
Sbjct: 318 EQMEIAKSKPAGELLNWDDLKKMKYSWNVACEVMRLSPPLQGGFREAITDFMFNGFSIPK 377

Query: 74  GWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFARVET 133
           GW+++W++  TH +   F  P KFDP RF+      PY F+ FG GPR+CPG E+AR+E 
Sbjct: 378 GWKLYWSANSTHKNAECFPMPEKFDPTRFEGNGP-APYTFVPFGGGPRMCPGKEYARLEI 437

Query: 134 LVTIHYLITQFTW-KLLLDDHFIRDPMPTPTKGMPIKIWP 173
           LV +H L+ +F W K++ D+  I DP P P K +PI+++P
Sbjct: 438 LVFMHNLVKRFKWEKVIPDEKIIVDPFPIPAKDLPIRLYP 476

[Close]

BLAST of Cucsat.G15307.T4 vs. NCBI nr
Match: XP_004140824.1 (cytochrome P450 716B1 [Cucumis sativus] >KGN57442.1 hypothetical protein Csa_010272 [Cucumis sativus])

HSP 1 Score: 365 bits (937), Expect = 5.20e-123
Identity = 167/170 (98.24%), Postives = 169/170 (99.41%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL
Sbjct: 309 VLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 368

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 130
           IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR
Sbjct: 369 IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 428

Query: 131 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV 180
           VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV
Sbjct: 429 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV 478

[Close]

BLAST of Cucsat.G15307.T4 vs. NCBI nr
Match: XP_008457111.1 (PREDICTED: cytochrome P450 716B1-like [Cucumis melo] >KAA0047356.1 cytochrome P450 716B1-like protein [Cucumis melo var. makuwa] >TYK14034.1 cytochrome P450 716B1-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 351 bits (901), Expect = 1.88e-117
Identity = 161/169 (95.27%), Postives = 165/169 (97.63%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL
Sbjct: 309 VLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 368

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 130
           IPKGWQIFWTSPVTHLDD IFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR
Sbjct: 369 IPKGWQIFWTSPVTHLDDNIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 428

Query: 131 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMA 179
           VETLVTIHYLITQFTWKL+ DDHFIRDPMPTPTKGMPIKIWPR Q+FMA
Sbjct: 429 VETLVTIHYLITQFTWKLISDDHFIRDPMPTPTKGMPIKIWPR-QTFMA 476

[Close]

BLAST of Cucsat.G15307.T4 vs. NCBI nr
Match: XP_038899130.1 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Benincasa hispida])

HSP 1 Score: 322 bits (824), Expect = 7.56e-106
Identity = 149/174 (85.63%), Postives = 155/174 (89.08%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ EQEEIARSKKCGELL WEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDI+FGGYL
Sbjct: 308 VLQEQEEIARSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIEFGGYL 367

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPG----- 130
           IPKGWQIFW SPVTHLD TIFREP KFDP+RF NP SIPPYCF+GFG GPRICPG     
Sbjct: 368 IPKGWQIFWASPVTHLDGTIFREPLKFDPNRFDNPTSIPPYCFVGFGGGPRICPGGPRIC 427

Query: 131 --NEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSF 177
             NEFARVETLVTIHYLIT+FTWKLLLDDHFIRDPMPTPTKGMPIKI PR  S 
Sbjct: 428 PGNEFARVETLVTIHYLITRFTWKLLLDDHFIRDPMPTPTKGMPIKICPRETSM 481

[Close]

BLAST of Cucsat.G15307.T4 vs. NCBI nr
Match: XP_022139355.1 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Momordica charantia])

HSP 1 Score: 308 bits (789), Expect = 1.26e-100
Identity = 138/167 (82.63%), Postives = 150/167 (89.82%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ EQEEIARSK+CGELL WEDLAKMKYTWRVALETLRLVAP+FGGFRKAM DI+FGGYL
Sbjct: 310 VLQEQEEIARSKECGELLTWEDLAKMKYTWRVALETLRLVAPVFGGFRKAMKDIEFGGYL 369

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 130
           IPKGWQIFW SP+THLDDTIF EP KFDP+RF+N AS+P YCF+ FG GPRICPG EFAR
Sbjct: 370 IPKGWQIFWASPMTHLDDTIFEEPLKFDPNRFENQASVPXYCFVAFGGGPRICPGYEFAR 429

Query: 131 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSF 177
           VETLVTIHYLITQFTWKLLLDD FIRDPMP PTKG+PIK+ PR  S 
Sbjct: 430 VETLVTIHYLITQFTWKLLLDDLFIRDPMPVPTKGLPIKVCPRETSM 476

[Close]

BLAST of Cucsat.G15307.T4 vs. NCBI nr
Match: KAE8125584.1 (hypothetical protein FH972_020367 [Carpinus fangiana])

HSP 1 Score: 267 bits (683), Expect = 9.80e-88
Identity = 115/163 (70.55%), Postives = 140/163 (85.89%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ E EEIA++K  GE L WEDL KMKYTWRVA+ETLR+V P+FGGFRKA  DI++GGYL
Sbjct: 80  VLQEHEEIAKTKLSGEYLTWEDLTKMKYTWRVAMETLRMVPPVFGGFRKAQKDIEYGGYL 139

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 130
           IPKGWQIFWT+P+TH+D++IF+EPSKF P RF++ ASIPPY F+ FG GPRICPG EFAR
Sbjct: 140 IPKGWQIFWTTPMTHMDNSIFQEPSKFYPSRFESQASIPPYSFVAFGGGPRICPGYEFAR 199

Query: 131 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPR 173
           +ETLVTIHYL+TQFTWKL  D+ F RDP+P PT+G+PIK+ PR
Sbjct: 200 IETLVTIHYLVTQFTWKLCSDNTFSRDPIPIPTQGLPIKLVPR 242

[Close]

BLAST of Cucsat.G15307.T4 vs. ExPASy TrEMBL
Match: A0A0A0LA02 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G186690 PE=3 SV=1)

HSP 1 Score: 365 bits (937), Expect = 2.52e-123
Identity = 167/170 (98.24%), Postives = 169/170 (99.41%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL
Sbjct: 309 VLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 368

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 130
           IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR
Sbjct: 369 IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 428

Query: 131 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV 180
           VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV
Sbjct: 429 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMAV 478

[Close]

BLAST of Cucsat.G15307.T4 vs. ExPASy TrEMBL
Match: A0A5A7TZG7 (Cytochrome P450 716B1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold363G00020 PE=3 SV=1)

HSP 1 Score: 351 bits (901), Expect = 9.11e-118
Identity = 161/169 (95.27%), Postives = 165/169 (97.63%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL
Sbjct: 309 VLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 368

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 130
           IPKGWQIFWTSPVTHLDD IFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR
Sbjct: 369 IPKGWQIFWTSPVTHLDDNIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 428

Query: 131 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMA 179
           VETLVTIHYLITQFTWKL+ DDHFIRDPMPTPTKGMPIKIWPR Q+FMA
Sbjct: 429 VETLVTIHYLITQFTWKLISDDHFIRDPMPTPTKGMPIKIWPR-QTFMA 476

[Close]

BLAST of Cucsat.G15307.T4 vs. ExPASy TrEMBL
Match: A0A1S3C4V0 (cytochrome P450 716B1-like OS=Cucumis melo OX=3656 GN=LOC103496862 PE=3 SV=1)

HSP 1 Score: 351 bits (901), Expect = 9.11e-118
Identity = 161/169 (95.27%), Postives = 165/169 (97.63%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ EQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL
Sbjct: 309 VLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 368

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 130
           IPKGWQIFWTSPVTHLDD IFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR
Sbjct: 369 IPKGWQIFWTSPVTHLDDNIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 428

Query: 131 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSFMA 179
           VETLVTIHYLITQFTWKL+ DDHFIRDPMPTPTKGMPIKIWPR Q+FMA
Sbjct: 429 VETLVTIHYLITQFTWKLISDDHFIRDPMPTPTKGMPIKIWPR-QTFMA 476

[Close]

BLAST of Cucsat.G15307.T4 vs. ExPASy TrEMBL
Match: A0A6J1CC35 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like OS=Momordica charantia OX=3673 GN=LOC111010306 PE=3 SV=1)

HSP 1 Score: 308 bits (789), Expect = 6.10e-101
Identity = 138/167 (82.63%), Postives = 150/167 (89.82%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ EQEEIARSK+CGELL WEDLAKMKYTWRVALETLRLVAP+FGGFRKAM DI+FGGYL
Sbjct: 310 VLQEQEEIARSKECGELLTWEDLAKMKYTWRVALETLRLVAPVFGGFRKAMKDIEFGGYL 369

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 130
           IPKGWQIFW SP+THLDDTIF EP KFDP+RF+N AS+P YCF+ FG GPRICPG EFAR
Sbjct: 370 IPKGWQIFWASPMTHLDDTIFEEPLKFDPNRFENQASVPXYCFVAFGGGPRICPGYEFAR 429

Query: 131 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPRSQSF 177
           VETLVTIHYLITQFTWKLLLDD FIRDPMP PTKG+PIK+ PR  S 
Sbjct: 430 VETLVTIHYLITQFTWKLLLDDLFIRDPMPVPTKGLPIKVCPRETSM 476

[Close]

BLAST of Cucsat.G15307.T4 vs. ExPASy TrEMBL
Match: A0A5N6RXE7 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_020367 PE=3 SV=1)

HSP 1 Score: 267 bits (683), Expect = 4.75e-88
Identity = 115/163 (70.55%), Postives = 140/163 (85.89%), Query Frame = 0


Query: 11  LITEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIQFGGYL 70
           ++ E EEIA++K  GE L WEDL KMKYTWRVA+ETLR+V P+FGGFRKA  DI++GGYL
Sbjct: 80  VLQEHEEIAKTKLSGEYLTWEDLTKMKYTWRVAMETLRMVPPVFGGFRKAQKDIEYGGYL 139

Query: 71  IPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRICPGNEFAR 130
           IPKGWQIFWT+P+TH+D++IF+EPSKF P RF++ ASIPPY F+ FG GPRICPG EFAR
Sbjct: 140 IPKGWQIFWTTPMTHMDNSIFQEPSKFYPSRFESQASIPPYSFVAFGGGPRICPGYEFAR 199

Query: 131 VETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPR 173
           +ETLVTIHYL+TQFTWKL  D+ F RDP+P PT+G+PIK+ PR
Sbjct: 200 IETLVTIHYLVTQFTWKLCSDNTFSRDPIPIPTQGLPIKLVPR 242

The following BLAST results are available for this feature:

BLAST of Cucsat.G15307.T4 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A0S2II38	4.2e-50	51.88	Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=...	[more]
I7C6E8	2.3e-48	50.00	Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1	[more]
K4CEE8	4.3e-47	50.63	Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A46 PE=1 S...	[more]
A5BFI4	2.8e-46	51.25	Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2	[more]
Q2MJ20	3.6e-46	51.25	Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV...	[more]

BLAST of Cucsat.G15307.T4 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140824.1	5.20e-123	98.24	cytochrome P450 716B1 [Cucumis sativus] >KGN57442.1 hypothetical protein Csa_010...	[more]
XP_008457111.1	1.88e-117	95.27	PREDICTED: cytochrome P450 716B1-like [Cucumis melo] >KAA0047356.1 cytochrome P4...	[more]
XP_038899130.1	7.56e-106	85.63	LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Benincasa hispida]	[more]
XP_022139355.1	1.26e-100	82.63	LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Momordica charantia]	[more]
KAE8125584.1	9.80e-88	70.55	hypothetical protein FH972_020367 [Carpinus fangiana]	[more]

BLAST of Cucsat.G15307.T4 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LA02	2.52e-123	98.24	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G186690 PE=3 SV=1	[more]
A0A5A7TZG7	9.11e-118	95.27	Cytochrome P450 716B1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3C4V0	9.11e-118	95.27	cytochrome P450 716B1-like OS=Cucumis melo OX=3656 GN=LOC103496862 PE=3 SV=1	[more]
A0A6J1CC35	6.10e-101	82.63	LOW QUALITY PROTEIN: cytochrome P450 716B1-like OS=Momordica charantia OX=3673 G...	[more]
A0A5N6RXE7	4.75e-88	70.55	Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_020367 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002397	Cytochrome P450, B-class	PRINTS	PR00359	BP450	coord: 86..101 score: 34.17 coord: 114..123 score: 47.56 coord: 58..85 score: 23.89 coord: 123..134 score: 39.07 coord: 41..52 score: 31.48
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 11..172 e-value: 2.6E-46 score: 160.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 15..173
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 15..149 e-value: 2.5E-35 score: 122.1
None	No IPR available	PANTHER	PTHR24286:SF237	OS07G0520300 PROTEIN	coord: 11..173
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 11..173
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 116..125

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G15307	Cucsat.G15307	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G15307.T4.E1	Cucsat.G15307.T4.E1	exon
Cucsat.G15307.T4.E2	Cucsat.G15307.T4.E2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G15307.T4.C2	Cucsat.G15307.T4.C2	CDS
Cucsat.G15307.T4.C1	Cucsat.G15307.T4.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G15307.T4	Cucsat.G15307.T4-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen