Sed0016476.1 (mRNA) Chayote v1

Overview
NameSed0016476.1
TypemRNA
OrganismSechium edule (Chayote v1)
DescriptionRNase H domain-containing protein
LocationLG07: 18235620 .. 18236135 (+)
Sequence length516
RNA-Seq ExpressionSed0016476.1
SyntenySed0016476.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACCTTTTTACAAAATAAATACGGATGCGGCCACCAATATTAAGAGACGGAGAAGTGGTTATGGTGCTATTGCGCGGAATGAGGGAGGGGAGATTATGTATGCTATTTCACAACCAATAGAATGGGTGGTAGATGCAGAACTGGTGGAAGCTTTAGCTATTCGTAATAGTGTAGAATTTGCTTTGGATATGGGATTTCACTTTATTGAGGTGGAGTCCAATGCTCAAAGAGTTACTGGAATGTTAACTGGGAAAATTGATTGCAGAACAGAGGTGGAGCAAATTGTGGAGGAAACTAGAGTTCTTGCTCATTGTTTAAAGTCTGTCAAATTTTCCTGGTGTCGACATCAAGCAAATAAACTAGCACATTTCCTTGCAAAATTGGCGGCCCTTGGAGAGAATGCGAACTGGTGGATGGAGGAGTGTCCTCCGTGTATGTTGGATATTTATCGCTCTAAATTGGGGATTGCGACGAGTCGTGTTGTGGGTGCTGCCAACCTCGATTTTCTTTAA

mRNA sequence

ATGGCACCTTTTTACAAAATAAATACGGATGCGGCCACCAATATTAAGAGACGGAGAAGTGGTTATGGTGCTATTGCGCGGAATGAGGGAGGGGAGATTATGTATGCTATTTCACAACCAATAGAATGGGTGGTAGATGCAGAACTGGTGGAAGCTTTAGCTATTCGTAATAGTGTAGAATTTGCTTTGGATATGGGATTTCACTTTATTGAGGTGGAGTCCAATGCTCAAAGAGTTACTGGAATGTTAACTGGGAAAATTGATTGCAGAACAGAGGTGGAGCAAATTGTGGAGGAAACTAGAGTTCTTGCTCATTGTTTAAAGTCTGTCAAATTTTCCTGGTGTCGACATCAAGCAAATAAACTAGCACATTTCCTTGCAAAATTGGCGGCCCTTGGAGAGAATGCGAACTGGTGGATGGAGGAGTGTCCTCCGTGTATGTTGGATATTTATCGCTCTAAATTGGGGATTGCGACGAGTCGTGTTGTGGGTGCTGCCAACCTCGATTTTCTTTAA

Coding sequence (CDS)

ATGGCACCTTTTTACAAAATAAATACGGATGCGGCCACCAATATTAAGAGACGGAGAAGTGGTTATGGTGCTATTGCGCGGAATGAGGGAGGGGAGATTATGTATGCTATTTCACAACCAATAGAATGGGTGGTAGATGCAGAACTGGTGGAAGCTTTAGCTATTCGTAATAGTGTAGAATTTGCTTTGGATATGGGATTTCACTTTATTGAGGTGGAGTCCAATGCTCAAAGAGTTACTGGAATGTTAACTGGGAAAATTGATTGCAGAACAGAGGTGGAGCAAATTGTGGAGGAAACTAGAGTTCTTGCTCATTGTTTAAAGTCTGTCAAATTTTCCTGGTGTCGACATCAAGCAAATAAACTAGCACATTTCCTTGCAAAATTGGCGGCCCTTGGAGAGAATGCGAACTGGTGGATGGAGGAGTGTCCTCCGTGTATGTTGGATATTTATCGCTCTAAATTGGGGATTGCGACGAGTCGTGTTGTGGGTGCTGCCAACCTCGATTTTCTTTAA

Protein sequence

MAPFYKINTDAATNIKRRRSGYGAIARNEGGEIMYAISQPIEWVVDAELVEALAIRNSVEFALDMGFHFIEVESNAQRVTGMLTGKIDCRTEVEQIVEETRVLAHCLKSVKFSWCRHQANKLAHFLAKLAALGENANWWMEECPPCMLDIYRSKLGIATSRVVGAANLDFL
Homology
BLAST of Sed0016476.1 vs. NCBI nr
Match: XP_023904177.1 (uncharacterized protein LOC112015942 [Quercus suber])

HSP 1 Score: 103.2 bits (256), Expect = 2.3e-18
Identity = 57/147 (38.78%), Postives = 83/147 (56.46%), Query Frame = 0

Query: 3   PFYKINTDAATNIKRRRSGYGAIARNEGGEIMYAISQPIEWVVDAELVEALAIRNSVEFA 62
           PF+K+N DAA      RSG+GA+ RNE GE+M A+S     V  ++  E LA R ++EFA
Sbjct: 356 PFFKMNFDAAIFNDSGRSGFGAVIRNELGEVMAALSAIGPSVSSSDEAETLACRKAMEFA 415

Query: 63  LDMGFHFIEVESNAQRVTGMLTGKIDCRTEVEQIVEETRVLAHCLKSVKFSWCRHQANKL 122
           +D GF+ + +E ++  V   L+      + V  +V + + L   L+ V FSW     N++
Sbjct: 416 VDAGFNELIIEGDSINVIKALSSSSPDLSVVGNVVADIKCLISGLRRVSFSWVNRDCNRV 475

Query: 123 AHFLAKLAALGENANWWMEECPPCMLD 150
           AH LAK A   E   +WME+ PP  LD
Sbjct: 476 AHALAKFANSLEEDLYWMEDAPPVALD 502

BLAST of Sed0016476.1 vs. NCBI nr
Match: XP_023914985.1 (cytochrome P450 CYP82D47-like [Quercus suber])

HSP 1 Score: 97.1 bits (240), Expect = 1.6e-16
Identity = 51/167 (30.54%), Postives = 89/167 (53.29%), Query Frame = 0

Query: 4   FYKINTDAATNIKRRRSGYGAIARNEGGEIMYAISQPIEWVVDAELVEALAIRNSVEFAL 63
           ++K N D A   +   +G G +  +  G ++ A+S+ +E V +AE++E LAI+ ++ FA+
Sbjct: 8   WFKANVDGAIFKESNSAGIGVVVCDSQGWVLAALSEKVEGVQEAEVIETLAIQWAIRFAI 67

Query: 64  DMGFHFIEVESNAQRVTGMLTGKIDCRTEVEQIVEETRVLAHCLKSVKFSWCRHQANKLA 123
           +  F+ + +ES++  V   +    D    V  I+E+ + LA  LKS  F   + +AN++A
Sbjct: 68  ETSFNCVIIESDSLGVVKAIQDSADSTCHVGHIIEDVKQLAKALKSCDFQHTKREANQIA 127

Query: 124 HFLAKLAALGENANWWMEECPPCMLDIY------RSKLGIATSRVVG 165
           H L + A   +    W+EE PPC+ D+       R  L I T  + G
Sbjct: 128 HTLVRNAIKVDTVLAWLEEIPPCVADVVISDWIGRLSLNIITKVIAG 174

BLAST of Sed0016476.1 vs. NCBI nr
Match: XP_030945898.1 (uncharacterized protein LOC115970400 [Quercus lobata])

HSP 1 Score: 96.7 bits (239), Expect = 2.1e-16
Identity = 54/157 (34.39%), Postives = 81/157 (51.59%), Query Frame = 0

Query: 1   MAPFYKINTDAATNIKRRRSGYGAIARNEGGEIMYAISQPIEWVVDAELVEALAIRNSVE 60
           + P YK N DAA   +   +G G + R+ GG I+ A+S+ I+     EL EALA R +V 
Sbjct: 21  LEPVYKANFDAAMFEQLGLAGLGVVLRDSGGHIIAALSEKIKLPQTVELAEALAARRAVL 80

Query: 61  FALDMGFHFIEVESNAQRVTGMLTGKIDCRTEVEQIVEETRVLAHCLKSVKFSWCRHQAN 120
           FA +     + VE +  RV   L  +  C T    + EETR L  CL+   F   + + N
Sbjct: 81  FATEFSIFKVMVEGDCLRVIQALNEQGSCLTMFGHVAEETRRLGSCLELCSFHHVKREGN 140

Query: 121 KLAHFLAKLAALGENANWWMEECPPCMLDIYRSKLGI 158
           KLAH LA+ A L  + + W+EE P  +  +++    +
Sbjct: 141 KLAHSLARRAVLPADVDVWVEELPEDLDAVFQGDFAV 177

BLAST of Sed0016476.1 vs. NCBI nr
Match: XP_030936648.1 (uncharacterized protein LOC115961887 [Quercus lobata])

HSP 1 Score: 95.1 bits (235), Expect = 6.1e-16
Identity = 56/146 (38.36%), Postives = 83/146 (56.85%), Query Frame = 0

Query: 4   FYKINTDAATNIKRRRSGYGAIARNEGGEIMYAISQPIEWVVDAELVEALAIRNSVEFAL 63
           F+K+N D A   +   SGYGA+ RNE GE+M A++     V D+E VE +A R ++EFA+
Sbjct: 28  FFKLNFDGACFDEGAASGYGAVIRNEKGEVMAALAARGGAVRDSEEVEVMACRKALEFAI 87

Query: 64  DMGFHFIEVESNAQRVTGMLTGKIDCRTEVEQIVEETRVLAHCLKSVKFSWCRHQANKLA 123
           D GF  I +E N   V  M++      + +  I E+   LA   +S+ ++  R  AN +A
Sbjct: 88  DAGFTEIVLEGNNALVMKMISQAQPDLSRLGLIYEDIWCLAAGFRSISYNCIRRSANGVA 147

Query: 124 HFLAKLAALGENANWWMEECPPCMLD 150
           H LA+ A L +N   W+EE PP  +D
Sbjct: 148 HSLARFARLLDNEIVWLEEDPPPAVD 173

BLAST of Sed0016476.1 vs. NCBI nr
Match: XP_023887146.1 (uncharacterized protein LOC111999255 [Quercus suber])

HSP 1 Score: 94.7 bits (234), Expect = 8.0e-16
Identity = 55/150 (36.67%), Postives = 86/150 (57.33%), Query Frame = 0

Query: 6   KINTDAATNIKRRRSGYGAIARNEGGEIMYAISQPIEWVVDAELVEALAIRNSVEFALDM 65
           KIN D AT      +G GAI RN+ G +M A +  I      E+VE LA R+++ FA ++
Sbjct: 93  KINFDGATFSDLSSAGLGAIIRNDLGPVMAAFTHLIPLPTSVEMVEVLAARSALCFAKEL 152

Query: 66  GFHFIEVESNAQRVTGMLTGKIDCRTEVEQIVEETRVLAHCLKSVKFSWCRHQANKLAHF 125
           GF+ I VE +++ V   L+      + +  IV++ +VLA  L +V F+  R Q N++AH 
Sbjct: 153 GFNNIIVEGDSEIVIKALSTNGPSFSSIGHIVKDVKVLASSLGNVSFNHTRRQGNRVAHG 212

Query: 126 LAKLAALGENANWWMEECPPCMLDIYRSKL 156
           LA+LA    +   WME+ PP +  +Y S++
Sbjct: 213 LARLACNFIHFQSWMEDVPPAVEGVYASEI 242

BLAST of Sed0016476.1 vs. ExPASy TrEMBL
Match: A0A7N2LIC5 (RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 4.1e-18
Identity = 56/142 (39.44%), Postives = 77/142 (54.23%), Query Frame = 0

Query: 3   PFYKINTDAATNIKRRRSGYGAIARNEGGEIMYAISQPIEWVVDAELVEALAIRNSVEFA 62
           P YK N DAA       +G G + R+ GG ++ A+S+ I+     EL EALA R +V FA
Sbjct: 18  PVYKANFDAALFEHVGLAGLGVVLRDSGGNVIAALSEKIKLPQTVELAEALAARRAVIFA 77

Query: 63  LDMGFHFIEVESNAQRVTGMLTGKIDCRTEVEQIVEETRVLAHCLKSVKFSWCRHQANKL 122
            ++    + VE +  RV   L G+  C T    +VEETR L  CL+   F   + + NKL
Sbjct: 78  AELSIFKVMVEGDCLRVIQALNGQGSCLTMFGHVVEETRRLGSCLELCSFHHVKREGNKL 137

Query: 123 AHFLAKLAALGENANWWMEECP 145
           AH LA+ A L  N + W+EE P
Sbjct: 138 AHSLARRAVLSANVDVWVEELP 159

BLAST of Sed0016476.1 vs. ExPASy TrEMBL
Match: A0A2N9IYC8 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS57142 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 5.4e-18
Identity = 53/141 (37.59%), Postives = 79/141 (56.03%), Query Frame = 0

Query: 5    YKINTDAATNIKRRRSGYGAIARNEGGEIMYAISQPIEWVVDAELVEALAIRNSVEFALD 64
            YK+N D A    R  +G G I RN  GE+M A+SQ I +    E VEA A R++++FA D
Sbjct: 1519 YKVNYDGAVFNDRNEAGIGVIIRNHQGEVMGALSQRIPYPHSVEAVEASAARSAIQFAKD 1578

Query: 65   MGFHFIEVESNAQRVTGMLTGKIDCRTEVEQIVEETRVLAHCLKSVKFSWCRHQANKLAH 124
            +GF  I++E ++  +   +     C T    I+E+ R +A  L+SV+F   + + N +AH
Sbjct: 1579 LGFMNIDLEGDSTSIVEAILLTTPCSTIYGNIIEDIRQIARGLQSVQFLHIKREGNVMAH 1638

Query: 125  FLAKLAALGENANWWMEECPP 146
             LAK A + +    WME  PP
Sbjct: 1639 LLAKRAGINKPFETWMESVPP 1659

BLAST of Sed0016476.1 vs. ExPASy TrEMBL
Match: A0A7N2L9Q3 (RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 7.1e-18
Identity = 56/158 (35.44%), Postives = 83/158 (52.53%), Query Frame = 0

Query: 3   PFYKINTDAATNIKRRRSGYGAIARNEGGEIMYAISQPIEWVVDAELVEALAIRNSVEFA 62
           P YK N DAA   +   +G G + R+ GG I+ A+S+ I+     EL EALA R +V FA
Sbjct: 54  PVYKANFDAALFEQLGLAGLGVVLRDSGGHIIAALSEKIKLPQTVELAEALAARRAVLFA 113

Query: 63  LDMGFHFIEVESNAQRVTGMLTGKIDCRTEVEQIVEETRVLAHCLKSVKFSWCRHQANKL 122
            ++    + VE +  RV   L  +  C T    +VEETR L  CL+   F   + + NKL
Sbjct: 114 TELSIFKVMVEGDCLRVIQALNEQGSCLTMFGHVVEETRRLGSCLELCSFHHVKREGNKL 173

Query: 123 AHFLAKLAALGENANWWMEECPPCMLDIYRSKLGIATS 161
           AH LA+ A L  + + W+EE P  +  +++    +  S
Sbjct: 174 AHSLARRAVLSADVDVWVEELPEDLDAVFQGDCAVVVS 211

BLAST of Sed0016476.1 vs. ExPASy TrEMBL
Match: A0A2N9EYC3 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS7737 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 1.6e-17
Identity = 53/141 (37.59%), Postives = 78/141 (55.32%), Query Frame = 0

Query: 5    YKINTDAATNIKRRRSGYGAIARNEGGEIMYAISQPIEWVVDAELVEALAIRNSVEFALD 64
            YK+N D A    R  +G G I RN  GE+M A+SQ I +    E VEA A R++++FA D
Sbjct: 1476 YKVNYDGAVFNDRNEAGIGVIIRNHRGEVMGALSQRIPYPHSVEAVEASAARSAIQFAKD 1535

Query: 65   MGFHFIEVESNAQRVTGMLTGKIDCRTEVEQIVEETRVLAHCLKSVKFSWCRHQANKLAH 124
             GF  I++E ++  +   +     C T    I+E+ R +A  L+SV+F   + + N +AH
Sbjct: 1536 SGFMNIDLEGDSTSIVEAILLTTPCSTIYGNIIEDIRQIARGLQSVQFLHVKREGNVMAH 1595

Query: 125  FLAKLAALGENANWWMEECPP 146
             LAK A + +    WME  PP
Sbjct: 1596 LLAKRAGINKPFETWMETVPP 1616

BLAST of Sed0016476.1 vs. ExPASy TrEMBL
Match: A0A7N2N656 (RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 2.7e-17
Identity = 55/142 (38.73%), Postives = 77/142 (54.23%), Query Frame = 0

Query: 3   PFYKINTDAATNIKRRRSGYGAIARNEGGEIMYAISQPIEWVVDAELVEALAIRNSVEFA 62
           P YK N DAA       +G G + R+ GG ++ A+S+ I+     EL EALA R +V FA
Sbjct: 18  PVYKANFDAALFEHVGLAGLGVVLRDSGGNVIAALSEKIKLPQTVELAEALAARRAVIFA 77

Query: 63  LDMGFHFIEVESNAQRVTGMLTGKIDCRTEVEQIVEETRVLAHCLKSVKFSWCRHQANKL 122
            ++    + VE +  RV   L G+  C T    +VEETR L  CL+   F   + + NKL
Sbjct: 78  AELSIFKVMVEGDCLRVIQALNGQGSCLTMFGHVVEETRRLGSCLELCSFHHVKREGNKL 137

Query: 123 AHFLAKLAALGENANWWMEECP 145
           AH LA+ A L  + + W+EE P
Sbjct: 138 AHSLARRAVLSADVDVWVEEQP 159

BLAST of Sed0016476.1 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 48.1 bits (113), Expect = 8.0e-06
Identity = 34/123 (27.64%), Postives = 59/123 (47.97%), Query Frame = 0

Query: 6   KINTDAATNIKRRRSGYGAIARNEGGEIMYAISQPIEWVVDAELVEALAIRNSVEFALDM 65
           K NTDA   ++  R G G I RNE G +++  ++ +    +    E  A+R +V      
Sbjct: 146 KCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLEAELEALRWAVLTMSRF 205

Query: 66  GFHFIEVESNAQRVTGMLTGKIDCRTEVEQIVEETRVLAHCLKSVKFSWCRHQANKLAHF 125
            +  I  ES+AQ +  +L    D    ++  +E+ + L H  + VKF +     NK+A  
Sbjct: 206 NYKRIIFESDAQALVNLLNSD-DFWPTLQPALEDIQQLLHHFEEVKFEFTPRGGNKVADR 265

Query: 126 LAK 129
           +A+
Sbjct: 266 IAR 267

BLAST of Sed0016476.1 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 43.1 bits (100), Expect = 2.6e-04
Identity = 31/127 (24.41%), Postives = 60/127 (47.24%), Query Frame = 0

Query: 2   APFYKINTDAATNIKRRRSGYGAIARNEGGEIMYAISQPIEWVVDAELVEALAIRNSVEF 61
           A + K N DA  ++++  +  G I RN  G  +   S  +    +    E  A+  +++ 
Sbjct: 331 ATYVKCNFDAGFDVQKLEATGGWIIRNHYGTPISWGSMKLAHTSNPLEAETKALLAALQQ 390

Query: 62  ALDMGFHFIEVESNAQRVTGMLTGKIDCRTEVEQIVEETRVLAHCLKSVKFSWCRHQANK 121
               G+  + +E + Q +  ++ G I   + +   +E+    A+   S++F + R + NK
Sbjct: 391 TWIRGYTQVFMEGDCQTLINLING-ISFHSSLANHLEDISFWANKFASIQFGFIRRKGNK 450

Query: 122 LAHFLAK 129
           LAH LAK
Sbjct: 451 LAHVLAK 456

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023904177.12.3e-1838.78uncharacterized protein LOC112015942 [Quercus suber][more]
XP_023914985.11.6e-1630.54cytochrome P450 CYP82D47-like [Quercus suber][more]
XP_030945898.12.1e-1634.39uncharacterized protein LOC115970400 [Quercus lobata][more]
XP_030936648.16.1e-1638.36uncharacterized protein LOC115961887 [Quercus lobata][more]
XP_023887146.18.0e-1636.67uncharacterized protein LOC111999255 [Quercus suber][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A7N2LIC54.1e-1839.44RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
A0A2N9IYC85.4e-1837.59Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A7N2L9Q37.1e-1835.44RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
A0A2N9EYC31.6e-1737.59Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A7N2N6562.7e-1738.73RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G34320.18.0e-0627.64Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT3G09510.12.6e-0424.41Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 8..130
e-value: 1.7E-20
score: 73.1
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 5..136
e-value: 3.8E-15
score: 58.0
NoneNo IPR availablePANTHERPTHR47723OS05G0353850 PROTEINcoord: 3..154
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 7..128
e-value: 1.0539E-16
score: 70.0356
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 4..133

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Sed0016476Sed0016476gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0016476.1-exonSed0016476.1-exon-LG07:18235620..18236135exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0016476.1-cdsSed0016476.1-cds-LG07:18235620..18236135CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Sed0016476.1Sed0016476.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity