PI0021973.2 (mRNA) Melon (PI 482460) v1

Overview


Name	PI0021973.2
Type	mRNA
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	cytochrome b5-like
Location	chr05: 16573017 .. 16576646 (+)
Sequence length	999
RNA-Seq Expression	PI0021973.2
Synteny	PI0021973.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCCTCCTCCTTCTTCAAAAAAAAACAAAAACTGTCTTACACTGAACCAAACCAAAGAGATCAAGTGTCGGCTGTTTCCTGTTTGAACACAAACCAAGAAATCACTGAGGACCGGTAACTCACACCCACCACCAGAATTCAAATTCAATTCAATTCAATGCTTCTTCCCTGTCTCCCACATTGACGCATTTACTCTCTACTCTTTTTATTTCTGCACCGCCCTCGCTAGTTTCTGCACCGCCCTCACTAGTTTCTGCACTCCCCTTTGGTTCTCCCATTTCTCCGCCGTCTCCGGGCAAATTGGCAAATGGGGGGTGATGGAAAGCTCTTTACATTGGCTGAAGTCTCTCAGCACAATAATCCCAAGGACTGTTGGTTGATCATCAATGGCAAGGTTTTTCTTCATACCCTTTTCTTTGTTTTTGTTTTTGGGGTTTCTTCTTTTTATTATGCTTGCAGGAGTTTGATTCTCTGAAGTTGTTTCTTCTTCTGTTTTTGTTTTCTCTTGAAATCTCACATGCATTCTGGACAGACCCATTATTTTTCACTGTTGAATCAATATGTTTATCCATGGCTGCTAAACTGCTGCATGGTGAGCTTTGCCAATGATGTTGTATTGGGCTGGCTAATGAAGGAATTTAGTTGCAGAGTATAGTGTAAATTGAACTATTCCTTTACCTTTTTTCTTGGGAATCCAAAGGGATTCAGGCTTGATTTTGTAGCTTTTAAAATAATGGCAGCAAACGGTGCTTTAATACGAAGGAAATTAGTGTTTGGTGTATTTAAAACTTAAACGTGAAAAAGTAGGATAAAATAGGAGTTTCCAGGTGTTGGTTAGAAGGCATCATATTCTTTGGCATTATAATCAGGTTCACCAAACAATAAATACAGTTAGCATTCCCTCTAGTCAGTCACTTTTATCGTTGGCAAAATCAATTTCAAGCGCAGCCTCAATCGTTGAAGAGTGATGTTTCATCCTCACCGGGGCTTAGATGATTGATTTTTGGTTGAGTTGCTTTCATGATAGAAGTTGCTAGAGACTGAGCAAGGGGCATGCTACGAAACCAACAATAAAAAAAAAAAAGAAATCGACACAAAGATATATGTTGTTTACTAACAGTGTGTTAGCTACGTCTAGGATGTTGGCAGGTTAAAGGGTTTATATATAGCACACCCCTCTAAACTTTAGGGCCAAAACTGTAAATTACTTAAATGTGTATACAATACAACATAGGTTAAAAATGACTGGGAGCAAGACTCCATGATGGATCATTTGAAACACTAGATAACAGATTCAAGACATTCAAACATGGCAAATGCCTTAGTCACATGGTTTTTTTAAGTTGAAGAGCATGAGTTTGTGTAATATGTGTTAATAAGTTATGTAAAGGAATGGGGTTCTATAAATGTGTACTCATATCACGTATATTTGGTTTAGGAGTTTATGATCTATAAAATATTATACATAGTGTATTTTATAAGCCTGAGTATGTGTAACTTTGGCTAGTGTAGTTCATGAGGTTAACAAACAAATTTGCAGACGAAGATAAACAAACTCACATGATGGCAAACATAGACTTGGCTTCTTAATTCCAGATATATATTCCTTGCTCTGCTGTGCTCACCTTACCAAGTATATAGGACTGAACTGGAGATTTATGATTTATTGATCTTAAAATGCTCTGTGTTTAGTGTCTTGAATGGACTTATTTTGTGTCTTTGATTCCTTTTAGGACCAGGATCTCATTGTATGGATAGAATCTGAGCCTTCTTCATGTTTGGCAGTCCTGTTATGAAACAAATGCTCTCATATTTTTACCAATTCACTGACAGGATTATCAGTGTAGGAAAACTTTGTTTGAGAAGCGGTTAATATGAAGCTCAATAACTCGAAAATGAACTGATCCAAAGATCTCATTTTATTTTTGTGTGTGTGAATACTCTTGTCTACCGAGGGCACTCTTATGCATATACTATGTGAGAACTGGACTCCAGTGGGGGTTGGACATCCAAAACTTTGTTCAAACCTAACCAATAAAAAACCAGGCGTCTGGCGGATGAATGGTGCTATGCTGTCTGGTGGGTCAATGTTTGTCACTGCAGGCCTATCTGCCATAAAAAATTTCTTCATTCTTGATTCTTCTGATTCCATTTGGTGACTTTTTCCTTTGGCATCCATAATATGATCTCAAAGAAATGTTCTGAAGCCTCAGTCTGTTGAATTTGCACATTCTATTGATGTTAAAAATTTCTTGGAAATACCGACTTATGAAAGATTCATGACATATGAAGAGTTTTAAAATACCGACTTCCCCTACCTCGGCTTGAAAGTCCCAGATATTTCAAGTAGTATCTCTAGATTTAAAGTCCTTTACACTAGAGCCATCCTTTGGGTAAAATCAGAAATTTACTGTGTCCAATTAGTAGTGATAAGCTCTTGCTGGTTGTACTTGTTCATCAATGTGCTATAAACTCTGCTTTCGTCCTTATCAATGTAATGTGAAATCTCTTTCTTGGCCTACCATATCAATAAAGGATAAAGATACTCTAGGAATGCTTGTGTTGGAGAATGTCAAGTACTAGTTTCTCAACCAAAACTTGATAACCAACCATTAGCCAACTCAAGTTTCTTAAGGCCCTAAGTGTAAAGTAAGAGGCAGGAGGATAAACATGAAAATGCATGAAACTTCCCTCAAGTTAACCCGATTCCTACTCGGAAGTGAATATTAATTATATCTTCTCAAAGAACAACACTTGCGCATTTCGACTATTGTTTTGCTTCTAAAATTTCTTAAAGAATAGTAATAGAAAGTCGAATATTTTGATTTTCTCACTTGTACTATGAGCCCAGAATGCGATGGATAAGCTTTCAGTTGTTTTGGCAGTTGGCCTGGAATTAAACTAAATGAGTCGTGCACAGATTTTATCTTTGATGGGTACTGAATTTTTAGGTTGAGGTTACCATTTGTCCATCTTGATTATCTCAACAGGTTTACAATGTAACCGAGTTCTTGGAAGACCATCCTGGTGGCGACGAGGTGTTGTTGTCTGGCACAGGTAATAAATCTTCCAGTCATACAGCATTAGTTATTTACAATGTACATCGAGTGTTTGTGCATCGTTTACGCACTATGTAATTTTTATTACCACAGGTAAGGATGCAACTGATGATTTTGAAGACGTGGGTCATAGTGAGAGCGCAAGAGAAATGATGAGCAAATACTACGTTGGAGAGATCGACATCTCGACAATCCCAGAAAGGTCGACATACACAGCTCCAAAGCAGCCTCATTACAATCAGGATAAGACAACCGAATTCATCATTAAGCTCCTCCAGTTCTTGGTTCCTCTTGCAATCTTGGGATTGGCGGTCGGTATCCGCTTTTACAGCAAATCAAGCTGAGGTTCTTGTTGAGATATTAGGTGATGTAGTCTTATGATCAATTACCCAACCGCTGCATTGTGTTTGTAAAACTGAGAAATTTTCTAGTCCGATGCTCTACTTTTTTTTCTATTCCTTTTAGGTCTTTTACATTTTAGTTCTGCTAAAGTAAATGTTATTTTAGTTAAAGAAAAGAGTTGTTTGTAATCATGGAGTTGGTGGGTTTTATCAGTATTTTACTAAGGAAA

mRNA sequence

CTCCTCCTCCTTCTTCAAAAAAAAACAAAAACTGTCTTACACTGAACCAAACCAAAGAGATCAAGTGTCGGCTGTTTCCTGTTTGAACACAAACCAAGAAATCACTGAGGACCGGTAACTCACACCCACCACCAGAATTCAAATTCAATTCAATTCAATGCTTCTTCCCTGTCTCCCACATTGACGCATTTACTCTCTACTCTTTTTATTTCTGCACCGCCCTCGCTAGTTTCTGCACCGCCCTCACTAGTTTCTGCACTCCCCTTTGGTTCTCCCATTTCTCCGCCGTCTCCGGGCAAATTGGCAAATGGGGGGTGATGGAAAGCTCTTTACATTGGCTGAAGTCTCTCAGCACAATAATCCCAAGGACTGTTGGTTGATCATCAATGGCAAGACCCATTATTTTTCACTGTTGAATCAATATGTTTATCCATGGCTGCTAAACTGCTGCATGGTTTACAATGTAACCGAGTTCTTGGAAGACCATCCTGGTGGCGACGAGGTGTTGTTGTCTGGCACAGGTAAGGATGCAACTGATGATTTTGAAGACGTGGGTCATAGTGAGAGCGCAAGAGAAATGATGAGCAAATACTACGTTGGAGAGATCGACATCTCGACAATCCCAGAAAGGTCGACATACACAGCTCCAAAGCAGCCTCATTACAATCAGGATAAGACAACCGAATTCATCATTAAGCTCCTCCAGTTCTTGGTTCCTCTTGCAATCTTGGGATTGGCGGTCGGTATCCGCTTTTACAGCAAATCAAGCTGAGGTTCTTGTTGAGATATTAGGTGATGTAGTCTTATGATCAATTACCCAACCGCTGCATTGTGTTTGTAAAACTGAGAAATTTTCTAGTCCGATGCTCTACTTTTTTTTCTATTCCTTTTAGGTCTTTTACATTTTAGTTCTGCTAAAGTAAATGTTATTTTAGTTAAAGAAAAGAGTTGTTTGTAATCATGGAGTTGGTGGGTTTTATCAGTATTTTACTAAGGAAA

Coding sequence (CDS)

ATGGGGGGTGATGGAAAGCTCTTTACATTGGCTGAAGTCTCTCAGCACAATAATCCCAAGGACTGTTGGTTGATCATCAATGGCAAGACCCATTATTTTTCACTGTTGAATCAATATGTTTATCCATGGCTGCTAAACTGCTGCATGGTTTACAATGTAACCGAGTTCTTGGAAGACCATCCTGGTGGCGACGAGGTGTTGTTGTCTGGCACAGGTAAGGATGCAACTGATGATTTTGAAGACGTGGGTCATAGTGAGAGCGCAAGAGAAATGATGAGCAAATACTACGTTGGAGAGATCGACATCTCGACAATCCCAGAAAGGTCGACATACACAGCTCCAAAGCAGCCTCATTACAATCAGGATAAGACAACCGAATTCATCATTAAGCTCCTCCAGTTCTTGGTTCCTCTTGCAATCTTGGGATTGGCGGTCGGTATCCGCTTTTACAGCAAATCAAGCTGA

Protein sequence

MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYNQDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS

Homology

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BLAST of PI0021973.2 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 2.5e-56
Identity = 107/153 (69.93%), Postives = 121/153 (79.08%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MG + K+FTL+EVS+HN   DCW++INGK                    VYNVT+FLEDH
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGK--------------------VYNVTKFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGD+VLLS TGKDATDDFEDVGHSESAREMM +YYVGEID +TIP++  YT PKQPHYN
Sbjct: 61  PGGDDVLLSSTGKDATDDFEDVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKS 154
           QDKT+EFIIKLLQFLVPLAILGLAVGIR Y+KS
Sbjct: 121 QDKTSEFIIKLLQFLVPLAILGLAVGIRIYTKS 133

[Close]

BLAST of PI0021973.2 vs. ExPASy Swiss-Prot
Match: P49099 (Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.1e-54
Identity = 104/154 (67.53%), Postives = 116/154 (75.32%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGG  K+FTLAEVS HNN KDCWLII+GK                    VYNVT+FLEDH
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGK--------------------VYNVTKFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGG EVLLS TGKDATDDFED+GHS SAR M+ +YYVG+ID STIP +  YT PKQPHYN
Sbjct: 61  PGGGEVLLSATGKDATDDFEDIGHSSSARAMLDEYYVGDIDSSTIPTKVKYTPPKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFI+KLLQFLVPL ILG+A G+ FY+K S
Sbjct: 121 QDKTTEFIVKLLQFLVPLIILGVAFGVHFYTKQS 134

[Close]

BLAST of PI0021973.2 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.1e-54
Identity = 102/154 (66.23%), Postives = 120/154 (77.92%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGG+ K+FTLAEVSQHNN KDCWL+I+GK                    VY+VT+FL+DH
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGK--------------------VYDVTKFLDDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLS TGKDATDDFEDVGHS SAR M+ +YYVG+ID +TIP ++ YT P QPHYN
Sbjct: 61  PGGDEVLLSATGKDATDDFEDVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKT+EF++KLLQFLVPL ILG+A GIRFY+K S
Sbjct: 121 QDKTSEFVVKLLQFLVPLIILGVAFGIRFYTKQS 134

[Close]

BLAST of PI0021973.2 vs. ExPASy Swiss-Prot
Match: O04354 (Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 3.2e-51
Identity = 99/150 (66.00%), Postives = 114/150 (76.00%), Query Frame = 0


Query: 5   GKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDHPGGD 64
           GK+FTLAEV+QHNN KDCWLIINGK                    VY+VT+FLEDHPGGD
Sbjct: 2   GKIFTLAEVAQHNNSKDCWLIINGK--------------------VYDVTKFLEDHPGGD 61

Query: 65  EVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYNQDKT 124
           +VLLS TGKDATDDFED+GHS SA+ M+ +YYVG+ID S+IP +  YT PKQP YN DKT
Sbjct: 62  DVLLSATGKDATDDFEDIGHSSSAKAMLDEYYVGDIDSSSIPSQVKYTPPKQPLYNPDKT 121

Query: 125 TEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
            EF+IKLLQFLVPL IL  A+GIRFY+KSS
Sbjct: 122 REFVIKLLQFLVPLVILAGAIGIRFYTKSS 131

[Close]

BLAST of PI0021973.2 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 199.5 bits (506), Expect = 2.7e-50
Identity = 98/148 (66.22%), Postives = 112/148 (75.68%), Query Frame = 0


Query: 6   KLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDHPGGDE 65
           K++TL EV++HN+  DCWLII GK                    VYNV++FLEDHPGGD+
Sbjct: 7   KVYTLEEVAKHNSKDDCWLIIGGK--------------------VYNVSKFLEDHPGGDD 66

Query: 66  VLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYNQDKTT 125
           VLLS TGKDATDDFEDVGHS +AR MM +YYVG+ID STIP R+ Y  PKQPHYNQDKT 
Sbjct: 67  VLLSSTGKDATDDFEDVGHSTTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTP 126

Query: 126 EFIIKLLQFLVPLAILGLAVGIRFYSKS 154
           EFIIK+LQFLVPLAILGLAV IR Y+KS
Sbjct: 127 EFIIKILQFLVPLAILGLAVAIRIYTKS 134

[Close]

BLAST of PI0021973.2 vs. ExPASy TrEMBL
Match: A0A5D3CSF5 (Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G00160 PE=3 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.4e-65
Identity = 128/154 (83.12%), Postives = 132/154 (85.71%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGGDGKL+TLAEVSQHNNPKDCWLIINGK                    VYNVTEFLEDH
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGK--------------------VYNVTEFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYY+GEIDISTIPE+ TYT PKQPHYN
Sbjct: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFIIKLLQFLVPLAILGLAVGIRFY+KSS
Sbjct: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYNKSS 134

[Close]

BLAST of PI0021973.2 vs. ExPASy TrEMBL
Match: A0A1S3C5R9 (cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103496974 PE=3 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.4e-65
Identity = 128/154 (83.12%), Postives = 132/154 (85.71%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGGDGKL+TLAEVSQHNNPKDCWLIINGK                    VYNVTEFLEDH
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGK--------------------VYNVTEFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYY+GEIDISTIPE+ TYT PKQPHYN
Sbjct: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFIIKLLQFLVPLAILGLAVGIRFY+KSS
Sbjct: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYNKSS 134

[Close]

BLAST of PI0021973.2 vs. ExPASy TrEMBL
Match: A0A0A0LHS9 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G138200 PE=3 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 6.9e-65
Identity = 128/154 (83.12%), Postives = 130/154 (84.42%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGGDGKLFT AEVSQHNNPKDCWLIINGK                    VYNVTEFLEDH
Sbjct: 1   MGGDGKLFTFAEVSQHNNPKDCWLIINGK--------------------VYNVTEFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHSESARE MSKYYVGEIDIST+PER TYT PKQPHYN
Sbjct: 61  PGGDEVLLSGTGKDATDDFEDVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFIIKLLQFLVPLAILGLAVGIRFY+KSS
Sbjct: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYNKSS 134

[Close]

BLAST of PI0021973.2 vs. ExPASy TrEMBL
Match: A0A6J1CAX1 (cytochrome b5-like OS=Momordica charantia OX=3673 GN=LOC111009917 PE=3 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.5e-62
Identity = 122/154 (79.22%), Postives = 130/154 (84.42%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGGDGK+FTLAEVSQHN PKDCWLIINGK                    VYNVTEFLEDH
Sbjct: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGK--------------------VYNVTEFLEDH 109

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHS+SARE M+KYYVGEIDISTIPE+ TY+A KQPHYN
Sbjct: 110 PGGDEVLLSGTGKDATDDFEDVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYN 169

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFI+KLLQFLVPLAILGLAVGIRFY+KS+
Sbjct: 170 QDKTTEFIVKLLQFLVPLAILGLAVGIRFYTKST 183

[Close]

BLAST of PI0021973.2 vs. ExPASy TrEMBL
Match: A0A6J1KXV9 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111499200 PE=3 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.7e-61
Identity = 123/154 (79.87%), Postives = 128/154 (83.12%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGG GK+FTLAEVSQHN  +DCWLIINGK                    VYNVTEFLEDH
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGK--------------------VYNVTEFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHSESARE M+KYYVGEIDISTIPER TYTA KQPHYN
Sbjct: 61  PGGDEVLLSGTGKDATDDFEDVGHSESARESMNKYYVGEIDISTIPERLTYTASKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFI+KLLQFLVPLAILGLAVGIRFYSKS+
Sbjct: 121 QDKTTEFIVKLLQFLVPLAILGLAVGIRFYSKST 134

[Close]

BLAST of PI0021973.2 vs. NCBI nr
Match: XP_008457249.1 (PREDICTED: cytochrome b5-like [Cucumis melo] >KAA0033284.1 cytochrome b5-like [Cucumis melo var. makuwa] >TYK14823.1 cytochrome b5-like [Cucumis melo var. makuwa])

HSP 1 Score: 258.1 bits (658), Expect = 4.9e-65
Identity = 128/154 (83.12%), Postives = 132/154 (85.71%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGGDGKL+TLAEVSQHNNPKDCWLIINGK                    VYNVTEFLEDH
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGK--------------------VYNVTEFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYY+GEIDISTIPE+ TYT PKQPHYN
Sbjct: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFIIKLLQFLVPLAILGLAVGIRFY+KSS
Sbjct: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYNKSS 134

[Close]

BLAST of PI0021973.2 vs. NCBI nr
Match: XP_004138957.1 (cytochrome b5 [Cucumis sativus] >KGN61465.1 hypothetical protein Csa_005989 [Cucumis sativus])

HSP 1 Score: 256.5 bits (654), Expect = 1.4e-64
Identity = 128/154 (83.12%), Postives = 130/154 (84.42%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGGDGKLFT AEVSQHNNPKDCWLIINGK                    VYNVTEFLEDH
Sbjct: 1   MGGDGKLFTFAEVSQHNNPKDCWLIINGK--------------------VYNVTEFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHSESARE MSKYYVGEIDIST+PER TYT PKQPHYN
Sbjct: 61  PGGDEVLLSGTGKDATDDFEDVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFIIKLLQFLVPLAILGLAVGIRFY+KSS
Sbjct: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYNKSS 134

[Close]

BLAST of PI0021973.2 vs. NCBI nr
Match: XP_022138846.1 (cytochrome b5-like [Momordica charantia])

HSP 1 Score: 248.1 bits (632), Expect = 5.1e-62
Identity = 122/154 (79.22%), Postives = 130/154 (84.42%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGGDGK+FTLAEVSQHN PKDCWLIINGK                    VYNVTEFLEDH
Sbjct: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGK--------------------VYNVTEFLEDH 109

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHS+SARE M+KYYVGEIDISTIPE+ TY+A KQPHYN
Sbjct: 110 PGGDEVLLSGTGKDATDDFEDVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYN 169

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFI+KLLQFLVPLAILGLAVGIRFY+KS+
Sbjct: 170 QDKTTEFIVKLLQFLVPLAILGLAVGIRFYTKST 183

[Close]

BLAST of PI0021973.2 vs. NCBI nr
Match: XP_038906915.1 (cytochrome b5-like [Benincasa hispida])

HSP 1 Score: 247.7 bits (631), Expect = 6.7e-62
Identity = 127/154 (82.47%), Postives = 128/154 (83.12%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           M GD KLFTLAEVSQHNN KDCWLIINGK                    VYNVTEFLEDH
Sbjct: 1   MDGDRKLFTLAEVSQHNNRKDCWLIINGK--------------------VYNVTEFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKY VGEIDISTIPERSTYT  KQPHYN
Sbjct: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYSVGEIDISTIPERSTYTTSKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKS+
Sbjct: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKST 134

[Close]

BLAST of PI0021973.2 vs. NCBI nr
Match: XP_023006481.1 (cytochrome b5-like [Cucurbita maxima])

HSP 1 Score: 244.6 bits (623), Expect = 5.6e-61
Identity = 123/154 (79.87%), Postives = 128/154 (83.12%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGG GK+FTLAEVSQHN  +DCWLIINGK                    VYNVTEFLEDH
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGK--------------------VYNVTEFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHSESARE M+KYYVGEIDISTIPER TYTA KQPHYN
Sbjct: 61  PGGDEVLLSGTGKDATDDFEDVGHSESARESMNKYYVGEIDISTIPERLTYTASKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFI+KLLQFLVPLAILGLAVGIRFYSKS+
Sbjct: 121 QDKTTEFIVKLLQFLVPLAILGLAVGIRFYSKST 134

[Close]

BLAST of PI0021973.2 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 219.5 bits (558), Expect = 1.8e-57
Identity = 107/153 (69.93%), Postives = 121/153 (79.08%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MG + K+FTL+EVS+HN   DCW++INGK                    VYNVT+FLEDH
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGK--------------------VYNVTKFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGD+VLLS TGKDATDDFEDVGHSESAREMM +YYVGEID +TIP++  YT PKQPHYN
Sbjct: 61  PGGDDVLLSSTGKDATDDFEDVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKS 154
           QDKT+EFIIKLLQFLVPLAILGLAVGIR Y+KS
Sbjct: 121 QDKTSEFIIKLLQFLVPLAILGLAVGIRIYTKS 133

[Close]

BLAST of PI0021973.2 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 187.6 bits (475), Expect = 7.6e-48
Identity = 90/152 (59.21%), Postives = 111/152 (73.03%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           M  D K+ +  EVS+HN  KDCWLII+GK                    VY+VT F++DH
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGK--------------------VYDVTPFMDDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLS TGKDAT+DFEDVGHS++AR+MM KY++GEID S++P   TY AP+QP YN
Sbjct: 61  PGGDEVLLSSTGKDATNDFEDVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSK 153
           QDKT EFIIK+LQFLVP+ ILGLA+ +R Y+K
Sbjct: 121 QDKTPEFIIKILQFLVPILILGLALVVRHYTK 132

[Close]

BLAST of PI0021973.2 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 181.4 bits (459), Expect = 5.5e-46
Identity = 86/155 (55.48%), Postives = 115/155 (74.19%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGGDGK+FTL+EVSQH++ KDCW++I+GK                    VY+VT+FL+DH
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGK--------------------VYDVTKFLDDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPH-- 120
           PGGDEV+L+ TGKDATDDFEDVGHS +A+ M+ +YYVG+ID +T+P ++ +  P      
Sbjct: 61  PGGDEVILTSTGKDATDDFEDVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAV 120

Query: 121 YNQDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKS 154
             QDK+++F+IKLLQFLVPL ILGLA GIR+Y+K+
Sbjct: 121 ATQDKSSDFVIKLLQFLVPLLILGLAFGIRYYTKT 135

[Close]

BLAST of PI0021973.2 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 118.6 bits (296), Expect = 4.3e-27
Identity = 60/147 (40.82%), Postives = 89/147 (60.54%), Query Frame = 0


Query: 7   LFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDHPGGDEV 66
           L +  +V++H    DCW++I+GK                    VY+++ F+++HPGGD V
Sbjct: 4   LISFHDVAKHKCKNDCWILIHGK--------------------VYDISTFMDEHPGGDNV 63

Query: 67  LLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYNQDKTT- 126
           LL+ TGKDA+ DFEDV HS+ A+E+M KY +G++D ST+P    Y  P +      +TT 
Sbjct: 64  LLAVTGKDASIDFEDVNHSKDAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTK 123

Query: 127 -EFIIKLLQFLVPLAILGLAVGIRFYS 152
            E   KLL +L+PL ILG+A  +RFY+
Sbjct: 124 EESGKKLLIYLIPLLILGVAFALRFYN 130

[Close]

BLAST of PI0021973.2 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 115.9 bits (289), Expect = 2.8e-26
Identity = 56/147 (38.10%), Postives = 91/147 (61.90%), Query Frame = 0


Query: 6   KLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDHPGGDE 65
           KL+++ E + HN   DCW++I+GK                    VY+V+ ++++HPGGD+
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGK--------------------VYDVSSYMDEHPGGDD 65

Query: 66  VLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYNQDKTT 125
           VLL+  GKDATDDFED GHS+ ARE+M KY++GE+D S++PE      P+   Y +D+  
Sbjct: 66  VLLAVAGKDATDDFEDAGHSKDARELMEKYFIGELDESSLPE-----IPELKIYKKDQPQ 125

Query: 126 EFIIKLLQ-----FLVPLAILGLAVGI 148
           + + KL       ++VP++I+ ++V +
Sbjct: 126 DSVQKLFDLTKQYWVVPVSIITISVAV 127

The following BLAST results are available for this feature:

BLAST of PI0021973.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48845	2.5e-56	69.93	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]
P49099	1.1e-54	67.53	Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P49098	1.1e-54	66.23	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
O04354	3.2e-51	66.00	Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1	[more]
P49100	2.7e-50	66.22	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]

BLAST of PI0021973.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CSF5	2.4e-65	83.12	Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G0...	[more]
A0A1S3C5R9	2.4e-65	83.12	cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103496974 PE=3 SV=1	[more]
A0A0A0LHS9	6.9e-65	83.12	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A6J1CAX1	2.5e-62	79.22	cytochrome b5-like OS=Momordica charantia OX=3673 GN=LOC111009917 PE=3 SV=1	[more]
A0A6J1KXV9	2.7e-61	79.87	cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111499200 PE=3 SV=1	[more]

BLAST of PI0021973.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008457249.1	4.9e-65	83.12	PREDICTED: cytochrome b5-like [Cucumis melo] >KAA0033284.1 cytochrome b5-like [C...	[more]
XP_004138957.1	1.4e-64	83.12	cytochrome b5 [Cucumis sativus] >KGN61465.1 hypothetical protein Csa_005989 [Cuc...	[more]
XP_022138846.1	5.1e-62	79.22	cytochrome b5-like [Momordica charantia]	[more]
XP_038906915.1	6.7e-62	82.47	cytochrome b5-like [Benincasa hispida]	[more]
XP_023006481.1	5.6e-61	79.87	cytochrome b5-like [Cucurbita maxima]	[more]

BLAST of PI0021973.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32720.1	1.8e-57	69.93	cytochrome B5 isoform B	[more]
AT5G53560.1	7.6e-48	59.21	cytochrome B5 isoform E	[more]
AT5G48810.1	5.5e-46	55.48	cytochrome B5 isoform D	[more]
AT2G46650.1	4.3e-27	40.82	cytochrome B5 isoform C	[more]
AT1G26340.1	2.8e-26	38.10	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 88..100 score: 39.04 coord: 75..82 score: 48.11 coord: 50..60 score: 61.02 coord: 60..74 score: 53.54
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..101 e-value: 1.1E-27 score: 107.9
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 47..100 e-value: 1.1E-16 score: 60.7
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..101 score: 27.790995
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 3..105 e-value: 3.6E-32 score: 112.2
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 6..107
None	No IPR available	PIRSR	PIRSR005149-50	PIRSR005149-50	coord: 8..109 e-value: 3.1E-12 score: 43.9
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..31
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 42..152
None	No IPR available	PANTHER	PTHR19359:SF110	BNAC04G43300D PROTEIN	coord: 1..31
None	No IPR available	PANTHER	PTHR19359:SF110	BNAC04G43300D PROTEIN	coord: 42..152
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 56..63

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
PI0021973	PI0021973	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0021973.2-five_prime_utr	PI0021973.2-five_prime_utr-chr05:16573017..16573323	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0021973.2-exon	PI0021973.2-exon-chr05:16573017..16573410	exon
PI0021973.2-exon	PI0021973.2-exon-chr05:16573549..16573608	exon
PI0021973.2-exon	PI0021973.2-exon-chr05:16576007..16576073	exon
PI0021973.2-exon	PI0021973.2-exon-chr05:16576169..16576646	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0021973.2-cds	PI0021973.2-cds-chr05:16573324..16573410	CDS
PI0021973.2-cds	PI0021973.2-cds-chr05:16573549..16573608	CDS
PI0021973.2-cds	PI0021973.2-cds-chr05:16576007..16576073	CDS
PI0021973.2-cds	PI0021973.2-cds-chr05:16576169..16576419	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0021973.2-three_prime_utr	PI0021973.2-three_prime_utr-chr05:16576420..16576646	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
PI0021973.2	PI0021973.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043447	alkane biosynthetic process
biological_process	GO:0042761	very long-chain fatty acid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding