PI0021973.2 (mRNA) Melon (PI 482460) v1

Overview
NamePI0021973.2
TypemRNA
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptioncytochrome b5-like
Locationchr05: 16573017 .. 16576646 (+)
Sequence length999
RNA-Seq ExpressionPI0021973.2
SyntenyPI0021973.2
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCTCCTCCTTCTTCAAAAAAAAACAAAAACTGTCTTACACTGAACCAAACCAAAGAGATCAAGTGTCGGCTGTTTCCTGTTTGAACACAAACCAAGAAATCACTGAGGACCGGTAACTCACACCCACCACCAGAATTCAAATTCAATTCAATTCAATGCTTCTTCCCTGTCTCCCACATTGACGCATTTACTCTCTACTCTTTTTATTTCTGCACCGCCCTCGCTAGTTTCTGCACCGCCCTCACTAGTTTCTGCACTCCCCTTTGGTTCTCCCATTTCTCCGCCGTCTCCGGGCAAATTGGCAAATGGGGGGTGATGGAAAGCTCTTTACATTGGCTGAAGTCTCTCAGCACAATAATCCCAAGGACTGTTGGTTGATCATCAATGGCAAGGTTTTTCTTCATACCCTTTTCTTTGTTTTTGTTTTTGGGGTTTCTTCTTTTTATTATGCTTGCAGGAGTTTGATTCTCTGAAGTTGTTTCTTCTTCTGTTTTTGTTTTCTCTTGAAATCTCACATGCATTCTGGACAGACCCATTATTTTTCACTGTTGAATCAATATGTTTATCCATGGCTGCTAAACTGCTGCATGGTGAGCTTTGCCAATGATGTTGTATTGGGCTGGCTAATGAAGGAATTTAGTTGCAGAGTATAGTGTAAATTGAACTATTCCTTTACCTTTTTTCTTGGGAATCCAAAGGGATTCAGGCTTGATTTTGTAGCTTTTAAAATAATGGCAGCAAACGGTGCTTTAATACGAAGGAAATTAGTGTTTGGTGTATTTAAAACTTAAACGTGAAAAAGTAGGATAAAATAGGAGTTTCCAGGTGTTGGTTAGAAGGCATCATATTCTTTGGCATTATAATCAGGTTCACCAAACAATAAATACAGTTAGCATTCCCTCTAGTCAGTCACTTTTATCGTTGGCAAAATCAATTTCAAGCGCAGCCTCAATCGTTGAAGAGTGATGTTTCATCCTCACCGGGGCTTAGATGATTGATTTTTGGTTGAGTTGCTTTCATGATAGAAGTTGCTAGAGACTGAGCAAGGGGCATGCTACGAAACCAACAATAAAAAAAAAAAAGAAATCGACACAAAGATATATGTTGTTTACTAACAGTGTGTTAGCTACGTCTAGGATGTTGGCAGGTTAAAGGGTTTATATATAGCACACCCCTCTAAACTTTAGGGCCAAAACTGTAAATTACTTAAATGTGTATACAATACAACATAGGTTAAAAATGACTGGGAGCAAGACTCCATGATGGATCATTTGAAACACTAGATAACAGATTCAAGACATTCAAACATGGCAAATGCCTTAGTCACATGGTTTTTTTAAGTTGAAGAGCATGAGTTTGTGTAATATGTGTTAATAAGTTATGTAAAGGAATGGGGTTCTATAAATGTGTACTCATATCACGTATATTTGGTTTAGGAGTTTATGATCTATAAAATATTATACATAGTGTATTTTATAAGCCTGAGTATGTGTAACTTTGGCTAGTGTAGTTCATGAGGTTAACAAACAAATTTGCAGACGAAGATAAACAAACTCACATGATGGCAAACATAGACTTGGCTTCTTAATTCCAGATATATATTCCTTGCTCTGCTGTGCTCACCTTACCAAGTATATAGGACTGAACTGGAGATTTATGATTTATTGATCTTAAAATGCTCTGTGTTTAGTGTCTTGAATGGACTTATTTTGTGTCTTTGATTCCTTTTAGGACCAGGATCTCATTGTATGGATAGAATCTGAGCCTTCTTCATGTTTGGCAGTCCTGTTATGAAACAAATGCTCTCATATTTTTACCAATTCACTGACAGGATTATCAGTGTAGGAAAACTTTGTTTGAGAAGCGGTTAATATGAAGCTCAATAACTCGAAAATGAACTGATCCAAAGATCTCATTTTATTTTTGTGTGTGTGAATACTCTTGTCTACCGAGGGCACTCTTATGCATATACTATGTGAGAACTGGACTCCAGTGGGGGTTGGACATCCAAAACTTTGTTCAAACCTAACCAATAAAAAACCAGGCGTCTGGCGGATGAATGGTGCTATGCTGTCTGGTGGGTCAATGTTTGTCACTGCAGGCCTATCTGCCATAAAAAATTTCTTCATTCTTGATTCTTCTGATTCCATTTGGTGACTTTTTCCTTTGGCATCCATAATATGATCTCAAAGAAATGTTCTGAAGCCTCAGTCTGTTGAATTTGCACATTCTATTGATGTTAAAAATTTCTTGGAAATACCGACTTATGAAAGATTCATGACATATGAAGAGTTTTAAAATACCGACTTCCCCTACCTCGGCTTGAAAGTCCCAGATATTTCAAGTAGTATCTCTAGATTTAAAGTCCTTTACACTAGAGCCATCCTTTGGGTAAAATCAGAAATTTACTGTGTCCAATTAGTAGTGATAAGCTCTTGCTGGTTGTACTTGTTCATCAATGTGCTATAAACTCTGCTTTCGTCCTTATCAATGTAATGTGAAATCTCTTTCTTGGCCTACCATATCAATAAAGGATAAAGATACTCTAGGAATGCTTGTGTTGGAGAATGTCAAGTACTAGTTTCTCAACCAAAACTTGATAACCAACCATTAGCCAACTCAAGTTTCTTAAGGCCCTAAGTGTAAAGTAAGAGGCAGGAGGATAAACATGAAAATGCATGAAACTTCCCTCAAGTTAACCCGATTCCTACTCGGAAGTGAATATTAATTATATCTTCTCAAAGAACAACACTTGCGCATTTCGACTATTGTTTTGCTTCTAAAATTTCTTAAAGAATAGTAATAGAAAGTCGAATATTTTGATTTTCTCACTTGTACTATGAGCCCAGAATGCGATGGATAAGCTTTCAGTTGTTTTGGCAGTTGGCCTGGAATTAAACTAAATGAGTCGTGCACAGATTTTATCTTTGATGGGTACTGAATTTTTAGGTTGAGGTTACCATTTGTCCATCTTGATTATCTCAACAGGTTTACAATGTAACCGAGTTCTTGGAAGACCATCCTGGTGGCGACGAGGTGTTGTTGTCTGGCACAGGTAATAAATCTTCCAGTCATACAGCATTAGTTATTTACAATGTACATCGAGTGTTTGTGCATCGTTTACGCACTATGTAATTTTTATTACCACAGGTAAGGATGCAACTGATGATTTTGAAGACGTGGGTCATAGTGAGAGCGCAAGAGAAATGATGAGCAAATACTACGTTGGAGAGATCGACATCTCGACAATCCCAGAAAGGTCGACATACACAGCTCCAAAGCAGCCTCATTACAATCAGGATAAGACAACCGAATTCATCATTAAGCTCCTCCAGTTCTTGGTTCCTCTTGCAATCTTGGGATTGGCGGTCGGTATCCGCTTTTACAGCAAATCAAGCTGAGGTTCTTGTTGAGATATTAGGTGATGTAGTCTTATGATCAATTACCCAACCGCTGCATTGTGTTTGTAAAACTGAGAAATTTTCTAGTCCGATGCTCTACTTTTTTTTCTATTCCTTTTAGGTCTTTTACATTTTAGTTCTGCTAAAGTAAATGTTATTTTAGTTAAAGAAAAGAGTTGTTTGTAATCATGGAGTTGGTGGGTTTTATCAGTATTTTACTAAGGAAA

mRNA sequence

CTCCTCCTCCTTCTTCAAAAAAAAACAAAAACTGTCTTACACTGAACCAAACCAAAGAGATCAAGTGTCGGCTGTTTCCTGTTTGAACACAAACCAAGAAATCACTGAGGACCGGTAACTCACACCCACCACCAGAATTCAAATTCAATTCAATTCAATGCTTCTTCCCTGTCTCCCACATTGACGCATTTACTCTCTACTCTTTTTATTTCTGCACCGCCCTCGCTAGTTTCTGCACCGCCCTCACTAGTTTCTGCACTCCCCTTTGGTTCTCCCATTTCTCCGCCGTCTCCGGGCAAATTGGCAAATGGGGGGTGATGGAAAGCTCTTTACATTGGCTGAAGTCTCTCAGCACAATAATCCCAAGGACTGTTGGTTGATCATCAATGGCAAGACCCATTATTTTTCACTGTTGAATCAATATGTTTATCCATGGCTGCTAAACTGCTGCATGGTTTACAATGTAACCGAGTTCTTGGAAGACCATCCTGGTGGCGACGAGGTGTTGTTGTCTGGCACAGGTAAGGATGCAACTGATGATTTTGAAGACGTGGGTCATAGTGAGAGCGCAAGAGAAATGATGAGCAAATACTACGTTGGAGAGATCGACATCTCGACAATCCCAGAAAGGTCGACATACACAGCTCCAAAGCAGCCTCATTACAATCAGGATAAGACAACCGAATTCATCATTAAGCTCCTCCAGTTCTTGGTTCCTCTTGCAATCTTGGGATTGGCGGTCGGTATCCGCTTTTACAGCAAATCAAGCTGAGGTTCTTGTTGAGATATTAGGTGATGTAGTCTTATGATCAATTACCCAACCGCTGCATTGTGTTTGTAAAACTGAGAAATTTTCTAGTCCGATGCTCTACTTTTTTTTCTATTCCTTTTAGGTCTTTTACATTTTAGTTCTGCTAAAGTAAATGTTATTTTAGTTAAAGAAAAGAGTTGTTTGTAATCATGGAGTTGGTGGGTTTTATCAGTATTTTACTAAGGAAA

Coding sequence (CDS)

ATGGGGGGTGATGGAAAGCTCTTTACATTGGCTGAAGTCTCTCAGCACAATAATCCCAAGGACTGTTGGTTGATCATCAATGGCAAGACCCATTATTTTTCACTGTTGAATCAATATGTTTATCCATGGCTGCTAAACTGCTGCATGGTTTACAATGTAACCGAGTTCTTGGAAGACCATCCTGGTGGCGACGAGGTGTTGTTGTCTGGCACAGGTAAGGATGCAACTGATGATTTTGAAGACGTGGGTCATAGTGAGAGCGCAAGAGAAATGATGAGCAAATACTACGTTGGAGAGATCGACATCTCGACAATCCCAGAAAGGTCGACATACACAGCTCCAAAGCAGCCTCATTACAATCAGGATAAGACAACCGAATTCATCATTAAGCTCCTCCAGTTCTTGGTTCCTCTTGCAATCTTGGGATTGGCGGTCGGTATCCGCTTTTACAGCAAATCAAGCTGA

Protein sequence

MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYNQDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS
Homology
BLAST of PI0021973.2 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 2.5e-56
Identity = 107/153 (69.93%), Postives = 121/153 (79.08%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MG + K+FTL+EVS+HN   DCW++INGK                    VYNVT+FLEDH
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGK--------------------VYNVTKFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGD+VLLS TGKDATDDFEDVGHSESAREMM +YYVGEID +TIP++  YT PKQPHYN
Sbjct: 61  PGGDDVLLSSTGKDATDDFEDVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKS 154
           QDKT+EFIIKLLQFLVPLAILGLAVGIR Y+KS
Sbjct: 121 QDKTSEFIIKLLQFLVPLAILGLAVGIRIYTKS 133

BLAST of PI0021973.2 vs. ExPASy Swiss-Prot
Match: P49099 (Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.1e-54
Identity = 104/154 (67.53%), Postives = 116/154 (75.32%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGG  K+FTLAEVS HNN KDCWLII+GK                    VYNVT+FLEDH
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGK--------------------VYNVTKFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGG EVLLS TGKDATDDFED+GHS SAR M+ +YYVG+ID STIP +  YT PKQPHYN
Sbjct: 61  PGGGEVLLSATGKDATDDFEDIGHSSSARAMLDEYYVGDIDSSTIPTKVKYTPPKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFI+KLLQFLVPL ILG+A G+ FY+K S
Sbjct: 121 QDKTTEFIVKLLQFLVPLIILGVAFGVHFYTKQS 134

BLAST of PI0021973.2 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.1e-54
Identity = 102/154 (66.23%), Postives = 120/154 (77.92%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGG+ K+FTLAEVSQHNN KDCWL+I+GK                    VY+VT+FL+DH
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGK--------------------VYDVTKFLDDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLS TGKDATDDFEDVGHS SAR M+ +YYVG+ID +TIP ++ YT P QPHYN
Sbjct: 61  PGGDEVLLSATGKDATDDFEDVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKT+EF++KLLQFLVPL ILG+A GIRFY+K S
Sbjct: 121 QDKTSEFVVKLLQFLVPLIILGVAFGIRFYTKQS 134

BLAST of PI0021973.2 vs. ExPASy Swiss-Prot
Match: O04354 (Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 3.2e-51
Identity = 99/150 (66.00%), Postives = 114/150 (76.00%), Query Frame = 0

Query: 5   GKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDHPGGD 64
           GK+FTLAEV+QHNN KDCWLIINGK                    VY+VT+FLEDHPGGD
Sbjct: 2   GKIFTLAEVAQHNNSKDCWLIINGK--------------------VYDVTKFLEDHPGGD 61

Query: 65  EVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYNQDKT 124
           +VLLS TGKDATDDFED+GHS SA+ M+ +YYVG+ID S+IP +  YT PKQP YN DKT
Sbjct: 62  DVLLSATGKDATDDFEDIGHSSSAKAMLDEYYVGDIDSSSIPSQVKYTPPKQPLYNPDKT 121

Query: 125 TEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
            EF+IKLLQFLVPL IL  A+GIRFY+KSS
Sbjct: 122 REFVIKLLQFLVPLVILAGAIGIRFYTKSS 131

BLAST of PI0021973.2 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 199.5 bits (506), Expect = 2.7e-50
Identity = 98/148 (66.22%), Postives = 112/148 (75.68%), Query Frame = 0

Query: 6   KLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDHPGGDE 65
           K++TL EV++HN+  DCWLII GK                    VYNV++FLEDHPGGD+
Sbjct: 7   KVYTLEEVAKHNSKDDCWLIIGGK--------------------VYNVSKFLEDHPGGDD 66

Query: 66  VLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYNQDKTT 125
           VLLS TGKDATDDFEDVGHS +AR MM +YYVG+ID STIP R+ Y  PKQPHYNQDKT 
Sbjct: 67  VLLSSTGKDATDDFEDVGHSTTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTP 126

Query: 126 EFIIKLLQFLVPLAILGLAVGIRFYSKS 154
           EFIIK+LQFLVPLAILGLAV IR Y+KS
Sbjct: 127 EFIIKILQFLVPLAILGLAVAIRIYTKS 134

BLAST of PI0021973.2 vs. ExPASy TrEMBL
Match: A0A5D3CSF5 (Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G00160 PE=3 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.4e-65
Identity = 128/154 (83.12%), Postives = 132/154 (85.71%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGGDGKL+TLAEVSQHNNPKDCWLIINGK                    VYNVTEFLEDH
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGK--------------------VYNVTEFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYY+GEIDISTIPE+ TYT PKQPHYN
Sbjct: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFIIKLLQFLVPLAILGLAVGIRFY+KSS
Sbjct: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYNKSS 134

BLAST of PI0021973.2 vs. ExPASy TrEMBL
Match: A0A1S3C5R9 (cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103496974 PE=3 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.4e-65
Identity = 128/154 (83.12%), Postives = 132/154 (85.71%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGGDGKL+TLAEVSQHNNPKDCWLIINGK                    VYNVTEFLEDH
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGK--------------------VYNVTEFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYY+GEIDISTIPE+ TYT PKQPHYN
Sbjct: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFIIKLLQFLVPLAILGLAVGIRFY+KSS
Sbjct: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYNKSS 134

BLAST of PI0021973.2 vs. ExPASy TrEMBL
Match: A0A0A0LHS9 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G138200 PE=3 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 6.9e-65
Identity = 128/154 (83.12%), Postives = 130/154 (84.42%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGGDGKLFT AEVSQHNNPKDCWLIINGK                    VYNVTEFLEDH
Sbjct: 1   MGGDGKLFTFAEVSQHNNPKDCWLIINGK--------------------VYNVTEFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHSESARE MSKYYVGEIDIST+PER TYT PKQPHYN
Sbjct: 61  PGGDEVLLSGTGKDATDDFEDVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFIIKLLQFLVPLAILGLAVGIRFY+KSS
Sbjct: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYNKSS 134

BLAST of PI0021973.2 vs. ExPASy TrEMBL
Match: A0A6J1CAX1 (cytochrome b5-like OS=Momordica charantia OX=3673 GN=LOC111009917 PE=3 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.5e-62
Identity = 122/154 (79.22%), Postives = 130/154 (84.42%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGGDGK+FTLAEVSQHN PKDCWLIINGK                    VYNVTEFLEDH
Sbjct: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGK--------------------VYNVTEFLEDH 109

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHS+SARE M+KYYVGEIDISTIPE+ TY+A KQPHYN
Sbjct: 110 PGGDEVLLSGTGKDATDDFEDVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYN 169

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFI+KLLQFLVPLAILGLAVGIRFY+KS+
Sbjct: 170 QDKTTEFIVKLLQFLVPLAILGLAVGIRFYTKST 183

BLAST of PI0021973.2 vs. ExPASy TrEMBL
Match: A0A6J1KXV9 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111499200 PE=3 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.7e-61
Identity = 123/154 (79.87%), Postives = 128/154 (83.12%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGG GK+FTLAEVSQHN  +DCWLIINGK                    VYNVTEFLEDH
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGK--------------------VYNVTEFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHSESARE M+KYYVGEIDISTIPER TYTA KQPHYN
Sbjct: 61  PGGDEVLLSGTGKDATDDFEDVGHSESARESMNKYYVGEIDISTIPERLTYTASKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFI+KLLQFLVPLAILGLAVGIRFYSKS+
Sbjct: 121 QDKTTEFIVKLLQFLVPLAILGLAVGIRFYSKST 134

BLAST of PI0021973.2 vs. NCBI nr
Match: XP_008457249.1 (PREDICTED: cytochrome b5-like [Cucumis melo] >KAA0033284.1 cytochrome b5-like [Cucumis melo var. makuwa] >TYK14823.1 cytochrome b5-like [Cucumis melo var. makuwa])

HSP 1 Score: 258.1 bits (658), Expect = 4.9e-65
Identity = 128/154 (83.12%), Postives = 132/154 (85.71%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGGDGKL+TLAEVSQHNNPKDCWLIINGK                    VYNVTEFLEDH
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGK--------------------VYNVTEFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYY+GEIDISTIPE+ TYT PKQPHYN
Sbjct: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFIIKLLQFLVPLAILGLAVGIRFY+KSS
Sbjct: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYNKSS 134

BLAST of PI0021973.2 vs. NCBI nr
Match: XP_004138957.1 (cytochrome b5 [Cucumis sativus] >KGN61465.1 hypothetical protein Csa_005989 [Cucumis sativus])

HSP 1 Score: 256.5 bits (654), Expect = 1.4e-64
Identity = 128/154 (83.12%), Postives = 130/154 (84.42%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGGDGKLFT AEVSQHNNPKDCWLIINGK                    VYNVTEFLEDH
Sbjct: 1   MGGDGKLFTFAEVSQHNNPKDCWLIINGK--------------------VYNVTEFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHSESARE MSKYYVGEIDIST+PER TYT PKQPHYN
Sbjct: 61  PGGDEVLLSGTGKDATDDFEDVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFIIKLLQFLVPLAILGLAVGIRFY+KSS
Sbjct: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYNKSS 134

BLAST of PI0021973.2 vs. NCBI nr
Match: XP_022138846.1 (cytochrome b5-like [Momordica charantia])

HSP 1 Score: 248.1 bits (632), Expect = 5.1e-62
Identity = 122/154 (79.22%), Postives = 130/154 (84.42%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGGDGK+FTLAEVSQHN PKDCWLIINGK                    VYNVTEFLEDH
Sbjct: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGK--------------------VYNVTEFLEDH 109

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHS+SARE M+KYYVGEIDISTIPE+ TY+A KQPHYN
Sbjct: 110 PGGDEVLLSGTGKDATDDFEDVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYN 169

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFI+KLLQFLVPLAILGLAVGIRFY+KS+
Sbjct: 170 QDKTTEFIVKLLQFLVPLAILGLAVGIRFYTKST 183

BLAST of PI0021973.2 vs. NCBI nr
Match: XP_038906915.1 (cytochrome b5-like [Benincasa hispida])

HSP 1 Score: 247.7 bits (631), Expect = 6.7e-62
Identity = 127/154 (82.47%), Postives = 128/154 (83.12%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           M GD KLFTLAEVSQHNN KDCWLIINGK                    VYNVTEFLEDH
Sbjct: 1   MDGDRKLFTLAEVSQHNNRKDCWLIINGK--------------------VYNVTEFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKY VGEIDISTIPERSTYT  KQPHYN
Sbjct: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYSVGEIDISTIPERSTYTTSKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKS+
Sbjct: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKST 134

BLAST of PI0021973.2 vs. NCBI nr
Match: XP_023006481.1 (cytochrome b5-like [Cucurbita maxima])

HSP 1 Score: 244.6 bits (623), Expect = 5.6e-61
Identity = 123/154 (79.87%), Postives = 128/154 (83.12%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGG GK+FTLAEVSQHN  +DCWLIINGK                    VYNVTEFLEDH
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGK--------------------VYNVTEFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLSGTGKDATDDFEDVGHSESARE M+KYYVGEIDISTIPER TYTA KQPHYN
Sbjct: 61  PGGDEVLLSGTGKDATDDFEDVGHSESARESMNKYYVGEIDISTIPERLTYTASKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKSS 155
           QDKTTEFI+KLLQFLVPLAILGLAVGIRFYSKS+
Sbjct: 121 QDKTTEFIVKLLQFLVPLAILGLAVGIRFYSKST 134

BLAST of PI0021973.2 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 219.5 bits (558), Expect = 1.8e-57
Identity = 107/153 (69.93%), Postives = 121/153 (79.08%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MG + K+FTL+EVS+HN   DCW++INGK                    VYNVT+FLEDH
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGK--------------------VYNVTKFLEDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGD+VLLS TGKDATDDFEDVGHSESAREMM +YYVGEID +TIP++  YT PKQPHYN
Sbjct: 61  PGGDDVLLSSTGKDATDDFEDVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKS 154
           QDKT+EFIIKLLQFLVPLAILGLAVGIR Y+KS
Sbjct: 121 QDKTSEFIIKLLQFLVPLAILGLAVGIRIYTKS 133

BLAST of PI0021973.2 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 187.6 bits (475), Expect = 7.6e-48
Identity = 90/152 (59.21%), Postives = 111/152 (73.03%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           M  D K+ +  EVS+HN  KDCWLII+GK                    VY+VT F++DH
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGK--------------------VYDVTPFMDDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYN 120
           PGGDEVLLS TGKDAT+DFEDVGHS++AR+MM KY++GEID S++P   TY AP+QP YN
Sbjct: 61  PGGDEVLLSSTGKDATNDFEDVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYN 120

Query: 121 QDKTTEFIIKLLQFLVPLAILGLAVGIRFYSK 153
           QDKT EFIIK+LQFLVP+ ILGLA+ +R Y+K
Sbjct: 121 QDKTPEFIIKILQFLVPILILGLALVVRHYTK 132

BLAST of PI0021973.2 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 181.4 bits (459), Expect = 5.5e-46
Identity = 86/155 (55.48%), Postives = 115/155 (74.19%), Query Frame = 0

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDH 60
           MGGDGK+FTL+EVSQH++ KDCW++I+GK                    VY+VT+FL+DH
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGK--------------------VYDVTKFLDDH 60

Query: 61  PGGDEVLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPH-- 120
           PGGDEV+L+ TGKDATDDFEDVGHS +A+ M+ +YYVG+ID +T+P ++ +  P      
Sbjct: 61  PGGDEVILTSTGKDATDDFEDVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAV 120

Query: 121 YNQDKTTEFIIKLLQFLVPLAILGLAVGIRFYSKS 154
             QDK+++F+IKLLQFLVPL ILGLA GIR+Y+K+
Sbjct: 121 ATQDKSSDFVIKLLQFLVPLLILGLAFGIRYYTKT 135

BLAST of PI0021973.2 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 118.6 bits (296), Expect = 4.3e-27
Identity = 60/147 (40.82%), Postives = 89/147 (60.54%), Query Frame = 0

Query: 7   LFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDHPGGDEV 66
           L +  +V++H    DCW++I+GK                    VY+++ F+++HPGGD V
Sbjct: 4   LISFHDVAKHKCKNDCWILIHGK--------------------VYDISTFMDEHPGGDNV 63

Query: 67  LLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYNQDKTT- 126
           LL+ TGKDA+ DFEDV HS+ A+E+M KY +G++D ST+P    Y  P +      +TT 
Sbjct: 64  LLAVTGKDASIDFEDVNHSKDAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTK 123

Query: 127 -EFIIKLLQFLVPLAILGLAVGIRFYS 152
            E   KLL +L+PL ILG+A  +RFY+
Sbjct: 124 EESGKKLLIYLIPLLILGVAFALRFYN 130

BLAST of PI0021973.2 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 115.9 bits (289), Expect = 2.8e-26
Identity = 56/147 (38.10%), Postives = 91/147 (61.90%), Query Frame = 0

Query: 6   KLFTLAEVSQHNNPKDCWLIINGKTHYFSLLNQYVYPWLLNCCMVYNVTEFLEDHPGGDE 65
           KL+++ E + HN   DCW++I+GK                    VY+V+ ++++HPGGD+
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGK--------------------VYDVSSYMDEHPGGDD 65

Query: 66  VLLSGTGKDATDDFEDVGHSESAREMMSKYYVGEIDISTIPERSTYTAPKQPHYNQDKTT 125
           VLL+  GKDATDDFED GHS+ ARE+M KY++GE+D S++PE      P+   Y +D+  
Sbjct: 66  VLLAVAGKDATDDFEDAGHSKDARELMEKYFIGELDESSLPE-----IPELKIYKKDQPQ 125

Query: 126 EFIIKLLQ-----FLVPLAILGLAVGI 148
           + + KL       ++VP++I+ ++V +
Sbjct: 126 DSVQKLFDLTKQYWVVPVSIITISVAV 127

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O488452.5e-5669.93Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1[more]
P490991.1e-5467.53Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
P490981.1e-5466.23Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
O043543.2e-5166.00Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1[more]
P491002.7e-5066.22Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A5D3CSF52.4e-6583.12Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G0... [more]
A0A1S3C5R92.4e-6583.12cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103496974 PE=3 SV=1[more]
A0A0A0LHS96.9e-6583.12Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ... [more]
A0A6J1CAX12.5e-6279.22cytochrome b5-like OS=Momordica charantia OX=3673 GN=LOC111009917 PE=3 SV=1[more]
A0A6J1KXV92.7e-6179.87cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111499200 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008457249.14.9e-6583.12PREDICTED: cytochrome b5-like [Cucumis melo] >KAA0033284.1 cytochrome b5-like [C... [more]
XP_004138957.11.4e-6483.12cytochrome b5 [Cucumis sativus] >KGN61465.1 hypothetical protein Csa_005989 [Cuc... [more]
XP_022138846.15.1e-6279.22cytochrome b5-like [Momordica charantia][more]
XP_038906915.16.7e-6282.47cytochrome b5-like [Benincasa hispida][more]
XP_023006481.15.6e-6179.87cytochrome b5-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G32720.11.8e-5769.93cytochrome B5 isoform B [more]
AT5G53560.17.6e-4859.21cytochrome B5 isoform E [more]
AT5G48810.15.5e-4655.48cytochrome B5 isoform D [more]
AT2G46650.14.3e-2740.82cytochrome B5 isoform C [more]
AT1G26340.12.8e-2638.10cytochrome B5 isoform A [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001199Cytochrome b5-like heme/steroid binding domainPRINTSPR00363CYTOCHROMEB5coord: 88..100
score: 39.04
coord: 75..82
score: 48.11
coord: 50..60
score: 61.02
coord: 60..74
score: 53.54
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 8..101
e-value: 1.1E-27
score: 107.9
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 47..100
e-value: 1.1E-16
score: 60.7
IPR001199Cytochrome b5-like heme/steroid binding domainPROSITEPS50255CYTOCHROME_B5_2coord: 5..101
score: 27.790995
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilyGENE3D3.10.120.10coord: 3..105
e-value: 3.6E-32
score: 112.2
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilySUPERFAMILY55856Cytochrome b5-like heme/steroid binding domaincoord: 6..107
NoneNo IPR availablePIRSRPIRSR005149-50PIRSR005149-50coord: 8..109
e-value: 3.1E-12
score: 43.9
NoneNo IPR availablePANTHERPTHR19359CYTOCHROME B5coord: 1..31
NoneNo IPR availablePANTHERPTHR19359CYTOCHROME B5coord: 42..152
NoneNo IPR availablePANTHERPTHR19359:SF110BNAC04G43300D PROTEINcoord: 1..31
NoneNo IPR availablePANTHERPTHR19359:SF110BNAC04G43300D PROTEINcoord: 42..152
IPR018506Cytochrome b5, heme-binding sitePROSITEPS00191CYTOCHROME_B5_1coord: 56..63

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
PI0021973PI0021973gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
PI0021973.2-five_prime_utrPI0021973.2-five_prime_utr-chr05:16573017..16573323five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
PI0021973.2-exonPI0021973.2-exon-chr05:16573017..16573410exon
PI0021973.2-exonPI0021973.2-exon-chr05:16573549..16573608exon
PI0021973.2-exonPI0021973.2-exon-chr05:16576007..16576073exon
PI0021973.2-exonPI0021973.2-exon-chr05:16576169..16576646exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
PI0021973.2-cdsPI0021973.2-cds-chr05:16573324..16573410CDS
PI0021973.2-cdsPI0021973.2-cds-chr05:16573549..16573608CDS
PI0021973.2-cdsPI0021973.2-cds-chr05:16576007..16576073CDS
PI0021973.2-cdsPI0021973.2-cds-chr05:16576169..16576419CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
PI0021973.2-three_prime_utrPI0021973.2-three_prime_utr-chr05:16576420..16576646three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
PI0021973.2PI0021973.2-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043447 alkane biosynthetic process
biological_process GO:0042761 very long-chain fatty acid biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0020037 heme binding
molecular_function GO:0046872 metal ion binding