Lcy03g001750.1 (mRNA) Sponge gourd (P93075) v1

Overview


Name	Lcy03g001750.1
Type	mRNA
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	RNA helicase
Location	Chr03: 6155900 .. 6157869 (+)
Sequence length	816
RNA-Seq Expression	Lcy03g001750.1
Synteny	Lcy03g001750.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAAGAAGGAGAAGAAGATGAAGAACAAAGCTCTTGTGGAGGCCCTAGGTTCTGAGGAAGCTTTTGAGCAGAAGAGTGACGAGTTGGTTCTTTCGGACAAGAAGAAGAAGAAGAAGAGTAAGAGTGATAAAGAAAGCATGAAGCGGAAAGCTGTAGAAGTGGCTGACGACGAGGATAGGAGCGAGACGAGCTCGGAGTTAGGCGAGCCTGTGAATTCGAGGTTGAAGAGCGGAAAGGAAAAGAAGAGCTCGAAGAAGGCAAGGTGGTTGAGTCTGACGAGGACGATGTGGAGCAGGAGCCCGAGGATCCGAATGCTGTTTCTAGGTTTCTGATTTCGGAACCGCTGAAGGCGAGGCTAAAGGAGAAGGGAATTACCGCGTTGTTTCCGATTCAGGCTATGACGTTTGACACCGTATATGACGGGTCTGATTTGGTTAGACAGGCCAGAACGGGACAGGTATAATTCGTTTGGTTGATTTTTTATTTGTTTTTCTGAGACTGTTAATTAAATGTTACGCTTTCCTCTTTAATATGAAACATAGAATTGGTATTATTTGGATTCAGACCAGCCGCTGAAAAACTGCAATAGAATCGAAGGTCGAGAATTTTTCTATTAGAAACTTGATTTGAAGCTCTTTTTTTCATGAGAAAATTCTTTTTTTCCACCTGAATGGTAGACTACCAGGCTCGCACGTTAGATGTTATGTTTTAGTTGTTTCCTGTCATGCAACTAAAACATTGCACGGTTAACTTTGTCTTGTCAAAACTTCTGTTATGTATAATCTTCATGTCTTTTGTGCAGGGTAAAACATTAGCTTTTGTTTTGCCCATCTTGGAGTCTTTAGTAAATGGGCCTTCTAAATCTTCGCGAGTGATTGGATATGGAAGGTCTCCTAGTGTTATAGTACTTTTACCCACTAGGGAGTTGGCTGACCAGGTATGGTTTGAAGATTTTTTTAATTTACATTTGTAATCTGATTTCAATATTTTGAAAAGTTGTGTTTTGACTAGGTGTTCGAGGACTTCAAATTTTATGGTCTAGCATTAGAGTCTTGTTGTTTATGTGGAGGAATGCTTTATGGGCCCCAAGAAACTAAATGAAGAAGGGGGGTTGACATAGTTGTTGGAACACCTGGTCGTGTAAAGGTAGAAATTTACAGTGAATTATGGAAAGCTTTGTTAAATGATGGGAAAACTTTAGTTCTTGCTTACTGACTTTGGTGTAGGATCACATTAACAGAAATAATATTGATATACGGTCTCTAAAGTTTAGGGTCCTTGATGAAGCTGATGAAATGTTGAGGATGGGTTTTGTTGAAGATGTTGAATTTATTCTTGGTATAGTATTTGGAATCTTAATTAATTGTTACACTGCGAGAGTGAAATCTATTTGCCTTTATTTCTTGATATTATGTCATTTATATTTACCTGACGTGGGCAAATCTATTTTGGTGGATTTTTAGGCAAGGTGGAGGATGTAAACAAAGTTCAAACACTTCTTTTCAGTGCCACTTTACCAGACTGGGTGAAAGATGTCAGTATTTGAACATAATATACAGTTACCGGGCTTCTTAATTTTAAACCTGCAATATAAATTAGCTGAAAAATTTGCTACAAAATGTACTTTTCTTTTGAAACTGTTATTGACAATTTGTTTTGCATTATTAGATTGCCTCTAGGTTCCTTAAAGCAAACAAGAAAACTGCAGATCTTGTTGGTAATGGAAAATGAAGGCCAGTAAGGATGTTAGGCATATTGTTATTCCTTGCTCTGATTTAGAAAGGTCTCGTCTTATTCCTGATATCATTCTATAGCAGGTTAGTGGTCTGATTTAGAAAGGTCTCGTCTTATTCCTGATATACAACAGTCTCAACGTTCGGTAATTTTCTAATTATCTTTTTATGTGTTTTTTTCAGGATTGATTGTGTGGCCATTTCTTGCGCAGGCTAGTCTTATTTATGGTTGA

mRNA sequence

ATGGAAAAGAAGGAGAAGAAGATGAAGAACAAAGCTCTTGTGGAGGCCCTAGGTTCTGAGGAAGCTTTTGAGCAGAAGAGTGACGAGTTGGTTCTTTCGGACAAGAAGAAGAAGAAGAAGAGTAAGAGTGATAAAGAAAGCATGAAGCGGAAAGCTGTAGAAGTGGCTGACGACGAGGATAGGAGCGAGACGAGCTCGGAGTTAGGCGAGCCTGTGAATTCGAGGTTGAAGAGCGGAAAGGAAAAGAAGAGCTCGAAGAAGGCAAGGTTTCTGATTTCGGAACCGCTGAAGGCGAGGCTAAAGGAGAAGGGAATTACCGCGTTGTTTCCGATTCAGGCTATGACGTTTGACACCGTATATGACGGGTCTGATTTGGTTAGACAGGCCAGAACGGGACAGGGTAAAACATTAGCTTTTGTTTTGCCCATCTTGGAGTCTTTAGTAAATGGGCCTTCTAAATCTTCGCGAGTGATTGGATATGGAAGGTCTCCTAGTGTTATAGTACTTTTACCCACTAGGGAGTTGGCTGACCAGGATCACATTAACAGAAATAATATTGATATACGGTCTCTAAAGTTTAGGGTCCTTGATGAAGCTGATGAAATGTTGAGGATGGGTTTTGTTGAAGATGTTGAATTTATTCTTGGCAAGGTGGAGGATGTAAACAAAGTTCAAACACTTCTTTTCAGTGCCACTTTACCAGACTGGGTGAAAGATATTGCCTCTAGGTTCCTTAAAGCAAACAAGAAAACTGCAGATCTTGTTGGATTGATTGTGTGGCCATTTCTTGCGCAGGCTAGTCTTATTTATGGTTGA

Coding sequence (CDS)

ATGGAAAAGAAGGAGAAGAAGATGAAGAACAAAGCTCTTGTGGAGGCCCTAGGTTCTGAGGAAGCTTTTGAGCAGAAGAGTGACGAGTTGGTTCTTTCGGACAAGAAGAAGAAGAAGAAGAGTAAGAGTGATAAAGAAAGCATGAAGCGGAAAGCTGTAGAAGTGGCTGACGACGAGGATAGGAGCGAGACGAGCTCGGAGTTAGGCGAGCCTGTGAATTCGAGGTTGAAGAGCGGAAAGGAAAAGAAGAGCTCGAAGAAGGCAAGGTTTCTGATTTCGGAACCGCTGAAGGCGAGGCTAAAGGAGAAGGGAATTACCGCGTTGTTTCCGATTCAGGCTATGACGTTTGACACCGTATATGACGGGTCTGATTTGGTTAGACAGGCCAGAACGGGACAGGGTAAAACATTAGCTTTTGTTTTGCCCATCTTGGAGTCTTTAGTAAATGGGCCTTCTAAATCTTCGCGAGTGATTGGATATGGAAGGTCTCCTAGTGTTATAGTACTTTTACCCACTAGGGAGTTGGCTGACCAGGATCACATTAACAGAAATAATATTGATATACGGTCTCTAAAGTTTAGGGTCCTTGATGAAGCTGATGAAATGTTGAGGATGGGTTTTGTTGAAGATGTTGAATTTATTCTTGGCAAGGTGGAGGATGTAAACAAAGTTCAAACACTTCTTTTCAGTGCCACTTTACCAGACTGGGTGAAAGATATTGCCTCTAGGTTCCTTAAAGCAAACAAGAAAACTGCAGATCTTGTTGGATTGATTGTGTGGCCATTTCTTGCGCAGGCTAGTCTTATTTATGGTTGA

Protein sequence

MEKKEKKMKNKALVEALGSEEAFEQKSDELVLSDKKKKKKSKSDKESMKRKAVEVADDEDRSETSSELGEPVNSRLKSGKEKKSSKKARFLISEPLKARLKEKGITALFPIQAMTFDTVYDGSDLVRQARTGQGKTLAFVLPILESLVNGPSKSSRVIGYGRSPSVIVLLPTRELADQDHINRNNIDIRSLKFRVLDEADEMLRMGFVEDVEFILGKVEDVNKVQTLLFSATLPDWVKDIASRFLKANKKTADLVGLIVWPFLAQASLIYG

Homology

[Close]

BLAST of Lcy03g001750.1 vs. ExPASy Swiss-Prot
Match: Q39189 (DEAD-box ATP-dependent RNA helicase 7 OS=Arabidopsis thaliana OX=3702 GN=RH7 PE=1 SV=2)

HSP 1 Score: 245.0 bits (624), Expect = 1.0e-63
Identity = 159/308 (51.62%), Postives = 199/308 (64.61%), Query Frame = 0


Query: 2   EKKEKKMKNKALVEA--LGSEEAFEQKSDELVLSDKKKKKKSKSDKESMKRKAVEVADDE 61
           +K+EKKMK K  ++   L S++  +++  +L  SD+++ +K KS K+  KRKA E  +DE
Sbjct: 9   KKEEKKMKKKMALDTPELDSKKGKKEQKLKLSDSDEEESEKKKSKKKDKKRKASE-EEDE 68

Query: 62  DRSETSSELGEPVNSRLKSGKE----KKSSKKARFLISEPLKARLKEKGITALFPIQAMT 121
            +S++SSE  +  + ++K G E       +  ++F IS PL+ +LK  GI ALFPIQA T
Sbjct: 69  VKSDSSSE-KKKSSKKVKLGVEDVEVDNPNAVSKFRISAPLREKLKANGIEALFPIQAST 128

Query: 122 FDTVYDGSDLVRQARTGQGKTLAFVLPILESLVNGPSKSSRVIGYGRSPSVIVLLPTREL 181
           FD V DG+DLV +ARTGQGKTLAFVLPILESLVNGP+KS R +GYGRSPSV+VLLPTREL
Sbjct: 129 FDMVLDGADLVGRARTGQGKTLAFVLPILESLVNGPAKSKRKMGYGRSPSVLVLLPTREL 188

Query: 182 ADQ-----------------------------------------------DHINRNNIDI 241
           A Q                                               DHI R N+D 
Sbjct: 189 AKQVAADFDAYGGSLGLSSCCLYGGDSYPVQEGKLKRGVDIVVGTPGRIKDHIERQNLDF 248

Query: 242 RSLKFRVLDEADEMLRMGFVEDVEFILGKVEDVNKVQTLLFSATLPDWVKDIASRFLKAN 257
             L+FRVLDEADEMLRMGFVEDVE ILGKVED  KVQTLLFSATLP WVK+I++RFLK +
Sbjct: 249 SYLQFRVLDEADEMLRMGFVEDVELILGKVEDSTKVQTLLFSATLPSWVKNISNRFLKRD 308

[Close]

BLAST of Lcy03g001750.1 vs. ExPASy Swiss-Prot
Match: Q650T9 (DEAD-box ATP-dependent RNA helicase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0520700 PE=2 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 3.4e-56
Identity = 148/304 (48.68%), Postives = 172/304 (56.58%), Query Frame = 0


Query: 2   EKKEKKMKNKALVEALGSEEAFEQKSDELVLSDKKKKKKSKSDKESMKRKAVEVADDEDR 61
           EKKEKK K K    +  S    E++S     SD+      K+ KE  K K V   ++ED 
Sbjct: 41  EKKEKKRKAKEPSPSSSSSSEEEERSS--TSSDEPAPAAKKAKKEKTKEKVVVEEEEEDD 100

Query: 62  SETSSELGEPVNSRLKSGKEKKSSKK--ARFLISEPLKARLKEKGITALFPIQAMTFDTV 121
            E              SG E  +     A F ISE L+ +LK KGI ALFPIQA TFD V
Sbjct: 101 DE---------GELTASGDEDPADPNALANFRISESLREKLKSKGIKALFPIQATTFDLV 160

Query: 122 YDGSDLVRQARTGQGKTLAFVLPILESLVNGPSKSSRVIGYGRSPSVIVLLPTRELADQ- 181
            DG DLV +ARTGQGKTLAFVLPILESLVNG  K+SR   YGR P+V+VLLPTRELA Q 
Sbjct: 161 LDGHDLVGRARTGQGKTLAFVLPILESLVNGTHKASRRTDYGRPPTVLVLLPTRELAKQV 220

Query: 182 ----------------------------------------------DHINRNNIDIRSLK 241
                                                         D + +  ++ RSLK
Sbjct: 221 HTDFAFYGATFGLSACCVYGGSDYRSQEMAIRKGVDIVVGTPGRVKDFVEKGTLNFRSLK 280

Query: 242 FRVLDEADEMLRMGFVEDVEFILGKVEDVNKVQTLLFSATLPDWVKDIASRFLKANKKTA 257
           FRVLDEADEML MGFV+DVE ILGKVEDV KVQTLLFSAT+P+WVK ++ RFLK+ KKT 
Sbjct: 281 FRVLDEADEMLNMGFVDDVELILGKVEDVTKVQTLLFSATIPEWVKKLSLRFLKSGKKTV 333

[Close]

BLAST of Lcy03g001750.1 vs. ExPASy Swiss-Prot
Match: Q41382 (DEAD-box ATP-dependent RNA helicase 7 OS=Spinacia oleracea OX=3562 GN=RH7 PE=2 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 7.6e-56
Identity = 154/315 (48.89%), Postives = 186/315 (59.05%), Query Frame = 0


Query: 3   KKEKKMKNKALVEALGSEEAFEQKSDELVLSDKKKKKKS---KSDKE----SMKRKAVEV 62
           KKEKKMK+    E L S++   +K      S KKK+K S   K DKE      KRKAV++
Sbjct: 17  KKEKKMKS----ETLDSDDTVVKKEKS---SSKKKEKSSSSGKKDKEEKEKKKKRKAVDL 76

Query: 63  ADDEDRSETSSELGEPVNSRLKSGK--------EKKSSKKARFLISEPLKARLKEKGITA 122
            D  D+S+ SSEL +  + + K  K         +  +  + F IS+PLK  L  KGI A
Sbjct: 77  DDSSDKSDNSSELVQADDLKPKKAKVMEEAVVEAEDPNSLSNFRISKPLKDVLISKGIKA 136

Query: 123 LFPIQAMTFDTVYDGSDLVRQARTGQGKTLAFVLPILESLVNGPSKSSRVIGYGRSPSVI 182
           LFPIQAMTFD V DG DLV +ARTGQGKTLAFVLPI+ESLVNG +K  R  G+GR PSV+
Sbjct: 137 LFPIQAMTFDNVIDGCDLVGRARTGQGKTLAFVLPIVESLVNGRTKDLRRSGHGRLPSVL 196

Query: 183 VLLPTRELADQ-----------------------------------------------DH 242
           VLLPTRELA Q                                               D 
Sbjct: 197 VLLPTRELATQVLADFQVYGGAVGLTACSVYGGAPFHSQISSLTRGVDIVVGTPGRVKDL 256

Query: 243 INRNNIDIRSLKFRVLDEADEMLRMGFVEDVEFILGKVEDVNKVQTLLFSATLPDWVKDI 256
           + +  + + SL FRVLDEADEML+MGFV+DVE ILGKV+ V+KVQTLLFSATLP WVK I
Sbjct: 257 LEKGVLKLGSLLFRVLDEADEMLKMGFVDDVELILGKVDHVSKVQTLLFSATLPSWVKQI 316

[Close]

BLAST of Lcy03g001750.1 vs. ExPASy Swiss-Prot
Match: Q9BQ39 (ATP-dependent RNA helicase DDX50 OS=Homo sapiens OX=9606 GN=DDX50 PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 6.5e-31
Identity = 104/304 (34.21%), Postives = 145/304 (47.70%), Query Frame = 0


Query: 4   KEKKMKNKALVEALGSEEAFEQKSDELVLSDKKKKK---KSKSDKESMKRKAVEVADDED 63
           K+ KMK K   +   +EE F + SDE   S K ++K       D+   K K V   D   
Sbjct: 62  KKSKMKEKLNGD---TEEGFNRLSDEFSKSHKSRRKDLPNGDIDEYEKKSKRVSSLDTST 121

Query: 64  RSETSSELGEPVNSRLKSGKEKKSSKKARFLISEPLKARLKEKGITALFPIQAMTFDTVY 123
              + ++L E +       +E+K    + F ISE     LK +G+T LFPIQ  TF  VY
Sbjct: 122 HKSSDNKLEETLT------REQKEGAFSNFPISEETIKLLKGRGVTYLFPIQVKTFGPVY 181

Query: 124 DGSDLVRQARTGQGKTLAFVLPILESLVNGPSKSSRVIGYGRSPSVIVLLPTRELADQ-- 183
           +G DL+ QARTG GKT +F +P++E L     ++   I   RSP V+VL PTRELA+Q  
Sbjct: 182 EGKDLIAQARTGTGKTFSFAIPLIERL----QRNQETIKKSRSPKVLVLAPTRELANQVA 241

Query: 184 -------------------------------------------DHINRNNIDIRSLKFRV 243
                                                      DH+    +D+  L+  V
Sbjct: 242 KDFKDITRKLSVACFYGGTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVV 301

Query: 244 LDEADEMLRMGFVEDVEFILG---KVEDVNKVQTLLFSATLPDWVKDIASRFLKANKKTA 257
           LDE D+ML +GF E VE I+    K +  +  QTLLFSAT P WV  +A +++K+  +  
Sbjct: 302 LDEVDQMLDLGFAEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQV 352

[Close]

BLAST of Lcy03g001750.1 vs. ExPASy Swiss-Prot
Match: Q99MJ9 (ATP-dependent RNA helicase DDX50 OS=Mus musculus OX=10090 GN=Ddx50 PE=2 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 3.2e-30
Identity = 94/279 (33.69%), Postives = 137/279 (49.10%), Query Frame = 0


Query: 27  SDELVLSDKKKKKK-SKSDKESMKRKAVEVADDEDRSETSSELGEPVNSRLKSGKEKKSS 86
           SDE   S K ++K     D +  ++++  V+  E+  ++S +  E +       +E+K  
Sbjct: 81  SDEFSPSHKSRRKDLPNGDVDEYEKRSKRVSSSENSHKSSDKAEETLT------REQKEG 140

Query: 87  KKARFLISEPLKARLKEKGITALFPIQAMTFDTVYDGSDLVRQARTGQGKTLAFVLPILE 146
             + F ISE     LK +G+T LFPIQ  TF  VY+G DL+ QARTG GKT +F +P++E
Sbjct: 141 AFSNFSISEETIKLLKGRGVTYLFPIQVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIE 200

Query: 147 SLVNGPSKSSRVIGYGRSPSVIVLLPTRELADQ--------------------------- 206
            L     ++   I   RSP V+VL PTRELA+Q                           
Sbjct: 201 RL----QRNQETIKKSRSPKVLVLAPTRELANQVAKDFKDITRKLSVACFYGGTSYQSQI 260

Query: 207 ------------------DHINRNNIDIRSLKFRVLDEADEMLRMGFVEDVEFILG---K 257
                             DH+    +D+  L+  VLDE D+ML +GF E VE I+    K
Sbjct: 261 NQIRNGIDILVGTPGRIKDHLQSGRLDLSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYK 320

[Close]

BLAST of Lcy03g001750.1 vs. ExPASy TrEMBL
Match: A0A6J1JZR9 (RNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111491171 PE=3 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 3.5e-104
Identity = 229/317 (72.24%), Postives = 238/317 (75.08%), Query Frame = 0


Query: 8   MKNKALVEALGSEEAFEQKSDELVLSDKKKKKKSKSDKESMKRKAVEVADDEDRSETSSE 67
           MKNKALVEALG+EEAFEQ+SD+LVLSD KKKKKSK DKES KRKAVEVADDEDRSETSSE
Sbjct: 1   MKNKALVEALGTEEAFEQRSDDLVLSD-KKKKKSKGDKESKKRKAVEVADDEDRSETSSE 60

Query: 68  LGEPVNSRLKSGKEKKSSKK---------------------ARFLISEPLKARLKEKGIT 127
           LGEPVNSRLKSGKEKKSSKK                     +RF ISEPLKARLKEKGI 
Sbjct: 61  LGEPVNSRLKSGKEKKSSKKTKVVESDEDDAEKEVEDPNAVSRFRISEPLKARLKEKGII 120

Query: 128 ALFPIQAMTFDTVYDGSDLVRQARTGQGKTLAFVLPILESLVNGPSKSSRVIGYGRSPSV 187
           ALFPIQAMTFDTVYDG DLV +ARTGQGKTLAFVLPILE L+NGPSKSSR  G+GRSPSV
Sbjct: 121 ALFPIQAMTFDTVYDGFDLVGRARTGQGKTLAFVLPILECLINGPSKSSRGTGFGRSPSV 180

Query: 188 IVLLPTRELADQ-----------------------------------------------D 247
           IVLLPTRELADQ                                               D
Sbjct: 181 IVLLPTRELADQVFEDFKFYGHALGLESCCLCGGMLYGPQETKLRRGVDIVIGTPGRVKD 240

Query: 248 HINRNNIDIRSLKFRVLDEADEMLRMGFVEDVEFILGKVEDVNKVQTLLFSATLPDWVKD 257
           HINRNNID+RSLKFRVLDEADEMLRMGFV+DVEFILGKVEDVNKVQTLLFSATLPDWVK 
Sbjct: 241 HINRNNIDLRSLKFRVLDEADEMLRMGFVDDVEFILGKVEDVNKVQTLLFSATLPDWVKT 300

[Close]

BLAST of Lcy03g001750.1 vs. ExPASy TrEMBL
Match: A0A6J1H389 (RNA helicase OS=Cucurbita moschata OX=3662 GN=LOC111459276 PE=3 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 3.5e-104
Identity = 229/317 (72.24%), Postives = 238/317 (75.08%), Query Frame = 0


Query: 8   MKNKALVEALGSEEAFEQKSDELVLSDKKKKKKSKSDKESMKRKAVEVADDEDRSETSSE 67
           MKNKALVEALG+EEAFEQ+SD+LVLSD KKKKKSK DKES KRKAVEVADDEDRSETSSE
Sbjct: 1   MKNKALVEALGTEEAFEQRSDDLVLSD-KKKKKSKGDKESKKRKAVEVADDEDRSETSSE 60

Query: 68  LGEPVNSRLKSGKEKKSSKK---------------------ARFLISEPLKARLKEKGIT 127
           LGEPVNSRLKSGKEKKSSKK                     +RF ISEPLKARLKEKGI 
Sbjct: 61  LGEPVNSRLKSGKEKKSSKKTKVIESDEDDAEKEVEDPNAVSRFRISEPLKARLKEKGII 120

Query: 128 ALFPIQAMTFDTVYDGSDLVRQARTGQGKTLAFVLPILESLVNGPSKSSRVIGYGRSPSV 187
           ALFPIQAMTFDTVYDG DLV +ARTGQGKTLAFVLPILE L+NGPSKSSR  G+GRSPSV
Sbjct: 121 ALFPIQAMTFDTVYDGFDLVGRARTGQGKTLAFVLPILECLINGPSKSSRGTGFGRSPSV 180

Query: 188 IVLLPTRELADQ-----------------------------------------------D 247
           IVLLPTRELADQ                                               D
Sbjct: 181 IVLLPTRELADQVFEDFKFYGQALGLESCCLCGGMLYGPQETKLRRGVDIVIGTPGRVKD 240

Query: 248 HINRNNIDIRSLKFRVLDEADEMLRMGFVEDVEFILGKVEDVNKVQTLLFSATLPDWVKD 257
           HINRNNID+RSLKFRVLDEADEMLRMGFV+DVEFILGKVEDVNKVQTLLFSATLPDWVK 
Sbjct: 241 HINRNNIDLRSLKFRVLDEADEMLRMGFVDDVEFILGKVEDVNKVQTLLFSATLPDWVKT 300

[Close]

BLAST of Lcy03g001750.1 vs. ExPASy TrEMBL
Match: A0A6J1ESS9 (RNA helicase OS=Cucurbita moschata OX=3662 GN=LOC111435671 PE=3 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 4.6e-104
Identity = 229/324 (70.68%), Postives = 243/324 (75.00%), Query Frame = 0


Query: 1   MEKKEKKMKNKALVEALGSEEAFEQKSDELVLSDKKKKKKSKSDKESMKRKAVEVADDED 60
           M+KKEKKMKNKALVEAL  E+AFEQKSDELVLSD KKKKKSK DKES KRKAVE ADD D
Sbjct: 1   MDKKEKKMKNKALVEALPLEDAFEQKSDELVLSD-KKKKKSKGDKESKKRKAVEEADDGD 60

Query: 61  RSETSSELGEPVNSRLKSGKEKKSSKK---------------------ARFLISEPLKAR 120
           RSETSSELGEPVNSRLK+GKEKKSSKK                     ++F ISEPL+AR
Sbjct: 61  RSETSSELGEPVNSRLKNGKEKKSSKKSKVVESDEDDVEKEADDQNAISKFRISEPLRAR 120

Query: 121 LKEKGITALFPIQAMTFDTVYDGSDLVRQARTGQGKTLAFVLPILESLVNGPSKSSRVIG 180
           LKEKGI++LFPIQAMTFDTVYDG DLV +ARTGQGKTLAFVLPILES+VNGPSKSS+V G
Sbjct: 121 LKEKGISSLFPIQAMTFDTVYDGFDLVGRARTGQGKTLAFVLPILESIVNGPSKSSKVTG 180

Query: 181 YGRSPSVIVLLPTRELADQ----------------------------------------- 240
           YGR+PSVIVLLPTRELADQ                                         
Sbjct: 181 YGRTPSVIVLLPTRELADQVFEDFKFYGHALGLESCCLCGGMLYGPQENKLRRGVDIVVG 240

Query: 241 ------DHINRNNIDIRSLKFRVLDEADEMLRMGFVEDVEFILGKVEDVNKVQTLLFSAT 257
                 DHINRNNID+RSLKFRVLDEADEMLRMGFV+DVEFILGKVEDVNKVQTLLFSAT
Sbjct: 241 TPGRVKDHINRNNIDLRSLKFRVLDEADEMLRMGFVDDVEFILGKVEDVNKVQTLLFSAT 300

[Close]

BLAST of Lcy03g001750.1 vs. ExPASy TrEMBL
Match: A0A6J1I4T2 (RNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111469154 PE=3 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 1.0e-103
Identity = 228/324 (70.37%), Postives = 242/324 (74.69%), Query Frame = 0


Query: 1   MEKKEKKMKNKALVEALGSEEAFEQKSDELVLSDKKKKKKSKSDKESMKRKAVEVADDED 60
           M+KKEKKMKNKALVEAL SE+AFEQKSDELVLSD KKKKKSK DKES KRKAVE ADD D
Sbjct: 1   MDKKEKKMKNKALVEALPSEDAFEQKSDELVLSD-KKKKKSKGDKESKKRKAVEEADDGD 60

Query: 61  RSETSSELGEPVNSRLKSGKEKKSSKK---------------------ARFLISEPLKAR 120
           RSETSSELGEPVNSRLK+GKEKKSSKK                     ++F ISEPL+AR
Sbjct: 61  RSETSSELGEPVNSRLKNGKEKKSSKKSKVVESDEDDVEKEADDQNAVSKFRISEPLRAR 120

Query: 121 LKEKGITALFPIQAMTFDTVYDGSDLVRQARTGQGKTLAFVLPILESLVNGPSKSSRVIG 180
           LKEKGI +LFPIQA+TFDTVYDG DLV +ARTGQGKTLAFVLPILES+VNGPSKSS+V G
Sbjct: 121 LKEKGIISLFPIQALTFDTVYDGFDLVGRARTGQGKTLAFVLPILESIVNGPSKSSKVTG 180

Query: 181 YGRSPSVIVLLPTRELADQ----------------------------------------- 240
           YGR+PSVIVLLPTRELADQ                                         
Sbjct: 181 YGRTPSVIVLLPTRELADQVFEDFKFYGHALGLESCCLCGGMLYGPQENKLRRGVDIVVG 240

Query: 241 ------DHINRNNIDIRSLKFRVLDEADEMLRMGFVEDVEFILGKVEDVNKVQTLLFSAT 257
                 DHINRNNID+RSLKFRVLDEADEMLRMGFV+DVEFILGKVEDVNKVQTLLFSAT
Sbjct: 241 TPGRVKDHINRNNIDLRSLKFRVLDEADEMLRMGFVDDVEFILGKVEDVNKVQTLLFSAT 300

[Close]

BLAST of Lcy03g001750.1 vs. ExPASy TrEMBL
Match: A0A1S3CTB6 (RNA helicase OS=Cucumis melo OX=3656 GN=LOC103504655 PE=3 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 3.6e-101
Identity = 226/324 (69.75%), Postives = 237/324 (73.15%), Query Frame = 0


Query: 1   MEKKEKKMKNKALVEALGSEEAFEQKSDELVLSDKKKKKKSKSDKESMKRKAVEVADDED 60
           M+KKEKKMKNKALVEAL S + FEQ +D L LSD KKKKKSKSDKES KRKAVEVADD D
Sbjct: 1   MDKKEKKMKNKALVEALASNDVFEQNADGLDLSD-KKKKKSKSDKESKKRKAVEVADDGD 60

Query: 61  RSETSSELGEPVNSRLKSGKEKKSSKK---------------------ARFLISEPLKAR 120
           RSETSSELGEPVNSR KSGKEKKSSKK                      RF ISEPLKA+
Sbjct: 61  RSETSSELGEPVNSRSKSGKEKKSSKKVKVVDSEDDDVEKEAENPNAVTRFRISEPLKAK 120

Query: 121 LKEKGITALFPIQAMTFDTVYDGSDLVRQARTGQGKTLAFVLPILESLVNGPSKSSRVIG 180
           LKEKGI++LFPIQAMTFDTVYDGSDLV +ARTGQGKTLAFVLPILESLVNGP KSSRV G
Sbjct: 121 LKEKGISSLFPIQAMTFDTVYDGSDLVGRARTGQGKTLAFVLPILESLVNGPFKSSRVTG 180

Query: 181 YGRSPSVIVLLPTRELADQ----------------------------------------- 240
           YGRSPSVIVLLPTRELADQ                                         
Sbjct: 181 YGRSPSVIVLLPTRELADQVFEDFKFYGRALGLDSCCLCGGMQYGPQEIALKRGVDIVVG 240

Query: 241 ------DHINRNNIDIRSLKFRVLDEADEMLRMGFVEDVEFILGKVEDVNKVQTLLFSAT 257
                 DHINRNNID+RSLKFRVLDEADEML MGFV+DVEFILGKV DVNKVQTLLFSAT
Sbjct: 241 TPGRVKDHINRNNIDLRSLKFRVLDEADEMLNMGFVDDVEFILGKVADVNKVQTLLFSAT 300

[Close]

BLAST of Lcy03g001750.1 vs. NCBI nr
Match: XP_023551208.1 (DEAD-box ATP-dependent RNA helicase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 389.8 bits (1000), Expect = 1.9e-104
Identity = 230/324 (70.99%), Postives = 244/324 (75.31%), Query Frame = 0


Query: 1   MEKKEKKMKNKALVEALGSEEAFEQKSDELVLSDKKKKKKSKSDKESMKRKAVEVADDED 60
           M+KKEKKMKNKALVEAL SE+AFEQKSDELVLSD KKKKKSK DKES KRKAVE ADD D
Sbjct: 1   MDKKEKKMKNKALVEALPSEDAFEQKSDELVLSD-KKKKKSKGDKESKKRKAVEEADDGD 60

Query: 61  RSETSSELGEPVNSRLKSGKEKKSSKK---------------------ARFLISEPLKAR 120
           RSETSSELGEPVNSRLK+GKEKKSSKK                     ++F ISEPL+AR
Sbjct: 61  RSETSSELGEPVNSRLKNGKEKKSSKKSKVVESDEDDVEKEADDPNAVSKFRISEPLRAR 120

Query: 121 LKEKGITALFPIQAMTFDTVYDGSDLVRQARTGQGKTLAFVLPILESLVNGPSKSSRVIG 180
           LKEKGI++LFPIQAMTFDTVYDG DLV +ARTGQGKTLAFVLPILES+VNGPSKSS+V G
Sbjct: 121 LKEKGISSLFPIQAMTFDTVYDGFDLVGRARTGQGKTLAFVLPILESIVNGPSKSSKVTG 180

Query: 181 YGRSPSVIVLLPTRELADQ----------------------------------------- 240
           YGR+PSVIVLLPTRELADQ                                         
Sbjct: 181 YGRTPSVIVLLPTRELADQVFEDFKFYGHALGLESCCLCGGMLYGPQENKLRRGVDIVVG 240

Query: 241 ------DHINRNNIDIRSLKFRVLDEADEMLRMGFVEDVEFILGKVEDVNKVQTLLFSAT 257
                 DHINRNNID+RSLKFRVLDEADEMLRMGFV+DVEFILGKVEDVNKVQTLLFSAT
Sbjct: 241 TPGRVKDHINRNNIDLRSLKFRVLDEADEMLRMGFVDDVEFILGKVEDVNKVQTLLFSAT 300

[Close]

BLAST of Lcy03g001750.1 vs. NCBI nr
Match: KAG6579664.1 (DEAD-box ATP-dependent RNA helicase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 389.8 bits (1000), Expect = 1.9e-104
Identity = 230/324 (70.99%), Postives = 244/324 (75.31%), Query Frame = 0


Query: 1   MEKKEKKMKNKALVEALGSEEAFEQKSDELVLSDKKKKKKSKSDKESMKRKAVEVADDED 60
           M+KKEKKMKNKALVEAL SE+AFEQKSDELVLSD KKKKKSK DKES KRKAVE ADD D
Sbjct: 1   MDKKEKKMKNKALVEALPSEDAFEQKSDELVLSD-KKKKKSKGDKESKKRKAVEEADDGD 60

Query: 61  RSETSSELGEPVNSRLKSGKEKKSSKK---------------------ARFLISEPLKAR 120
           RSETSSELGEPVNSRLK+GKEKKSSKK                     ++F ISEPL+AR
Sbjct: 61  RSETSSELGEPVNSRLKNGKEKKSSKKSKVVESDEDDVEKEADDQNAVSKFRISEPLRAR 120

Query: 121 LKEKGITALFPIQAMTFDTVYDGSDLVRQARTGQGKTLAFVLPILESLVNGPSKSSRVIG 180
           LKEKGI++LFPIQAMTFDTVYDG DLV +ARTGQGKTLAFVLPILES+VNGPSKSS+V G
Sbjct: 121 LKEKGISSLFPIQAMTFDTVYDGFDLVGRARTGQGKTLAFVLPILESIVNGPSKSSKVTG 180

Query: 181 YGRSPSVIVLLPTRELADQ----------------------------------------- 240
           YGR+PSVIVLLPTRELADQ                                         
Sbjct: 181 YGRTPSVIVLLPTRELADQVFEDFKFYGHALGLESCCLCGGMLYGPQENKLRRGVDIVVG 240

Query: 241 ------DHINRNNIDIRSLKFRVLDEADEMLRMGFVEDVEFILGKVEDVNKVQTLLFSAT 257
                 DHINRNNID+RSLKFRVLDEADEMLRMGFV+DVEFILGKVEDVNKVQTLLFSAT
Sbjct: 241 TPGRVKDHINRNNIDLRSLKFRVLDEADEMLRMGFVDDVEFILGKVEDVNKVQTLLFSAT 300

[Close]

BLAST of Lcy03g001750.1 vs. NCBI nr
Match: XP_022957864.1 (DEAD-box ATP-dependent RNA helicase 7-like [Cucurbita moschata] >KAG6605682.1 DEAD-box ATP-dependent RNA helicase 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035590.1 DEAD-box ATP-dependent RNA helicase 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 387.9 bits (995), Expect = 7.3e-104
Identity = 229/317 (72.24%), Postives = 238/317 (75.08%), Query Frame = 0


Query: 8   MKNKALVEALGSEEAFEQKSDELVLSDKKKKKKSKSDKESMKRKAVEVADDEDRSETSSE 67
           MKNKALVEALG+EEAFEQ+SD+LVLSD KKKKKSK DKES KRKAVEVADDEDRSETSSE
Sbjct: 1   MKNKALVEALGTEEAFEQRSDDLVLSD-KKKKKSKGDKESKKRKAVEVADDEDRSETSSE 60

Query: 68  LGEPVNSRLKSGKEKKSSKK---------------------ARFLISEPLKARLKEKGIT 127
           LGEPVNSRLKSGKEKKSSKK                     +RF ISEPLKARLKEKGI 
Sbjct: 61  LGEPVNSRLKSGKEKKSSKKTKVIESDEDDAEKEVEDPNAVSRFRISEPLKARLKEKGII 120

Query: 128 ALFPIQAMTFDTVYDGSDLVRQARTGQGKTLAFVLPILESLVNGPSKSSRVIGYGRSPSV 187
           ALFPIQAMTFDTVYDG DLV +ARTGQGKTLAFVLPILE L+NGPSKSSR  G+GRSPSV
Sbjct: 121 ALFPIQAMTFDTVYDGFDLVGRARTGQGKTLAFVLPILECLINGPSKSSRGTGFGRSPSV 180

Query: 188 IVLLPTRELADQ-----------------------------------------------D 247
           IVLLPTRELADQ                                               D
Sbjct: 181 IVLLPTRELADQVFEDFKFYGQALGLESCCLCGGMLYGPQETKLRRGVDIVIGTPGRVKD 240

Query: 248 HINRNNIDIRSLKFRVLDEADEMLRMGFVEDVEFILGKVEDVNKVQTLLFSATLPDWVKD 257
           HINRNNID+RSLKFRVLDEADEMLRMGFV+DVEFILGKVEDVNKVQTLLFSATLPDWVK 
Sbjct: 241 HINRNNIDLRSLKFRVLDEADEMLRMGFVDDVEFILGKVEDVNKVQTLLFSATLPDWVKT 300

[Close]

BLAST of Lcy03g001750.1 vs. NCBI nr
Match: XP_022995722.1 (DEAD-box ATP-dependent RNA helicase 7-like [Cucurbita maxima])

HSP 1 Score: 387.9 bits (995), Expect = 7.3e-104
Identity = 229/317 (72.24%), Postives = 238/317 (75.08%), Query Frame = 0


Query: 8   MKNKALVEALGSEEAFEQKSDELVLSDKKKKKKSKSDKESMKRKAVEVADDEDRSETSSE 67
           MKNKALVEALG+EEAFEQ+SD+LVLSD KKKKKSK DKES KRKAVEVADDEDRSETSSE
Sbjct: 1   MKNKALVEALGTEEAFEQRSDDLVLSD-KKKKKSKGDKESKKRKAVEVADDEDRSETSSE 60

Query: 68  LGEPVNSRLKSGKEKKSSKK---------------------ARFLISEPLKARLKEKGIT 127
           LGEPVNSRLKSGKEKKSSKK                     +RF ISEPLKARLKEKGI 
Sbjct: 61  LGEPVNSRLKSGKEKKSSKKTKVVESDEDDAEKEVEDPNAVSRFRISEPLKARLKEKGII 120

Query: 128 ALFPIQAMTFDTVYDGSDLVRQARTGQGKTLAFVLPILESLVNGPSKSSRVIGYGRSPSV 187
           ALFPIQAMTFDTVYDG DLV +ARTGQGKTLAFVLPILE L+NGPSKSSR  G+GRSPSV
Sbjct: 121 ALFPIQAMTFDTVYDGFDLVGRARTGQGKTLAFVLPILECLINGPSKSSRGTGFGRSPSV 180

Query: 188 IVLLPTRELADQ-----------------------------------------------D 247
           IVLLPTRELADQ                                               D
Sbjct: 181 IVLLPTRELADQVFEDFKFYGHALGLESCCLCGGMLYGPQETKLRRGVDIVIGTPGRVKD 240

Query: 248 HINRNNIDIRSLKFRVLDEADEMLRMGFVEDVEFILGKVEDVNKVQTLLFSATLPDWVKD 257
           HINRNNID+RSLKFRVLDEADEMLRMGFV+DVEFILGKVEDVNKVQTLLFSATLPDWVK 
Sbjct: 241 HINRNNIDLRSLKFRVLDEADEMLRMGFVDDVEFILGKVEDVNKVQTLLFSATLPDWVKT 300

[Close]

BLAST of Lcy03g001750.1 vs. NCBI nr
Match: XP_022928900.1 (DEAD-box ATP-dependent RNA helicase 7-like [Cucurbita moschata])

HSP 1 Score: 387.5 bits (994), Expect = 9.5e-104
Identity = 229/324 (70.68%), Postives = 243/324 (75.00%), Query Frame = 0


Query: 1   MEKKEKKMKNKALVEALGSEEAFEQKSDELVLSDKKKKKKSKSDKESMKRKAVEVADDED 60
           M+KKEKKMKNKALVEAL  E+AFEQKSDELVLSD KKKKKSK DKES KRKAVE ADD D
Sbjct: 1   MDKKEKKMKNKALVEALPLEDAFEQKSDELVLSD-KKKKKSKGDKESKKRKAVEEADDGD 60

Query: 61  RSETSSELGEPVNSRLKSGKEKKSSKK---------------------ARFLISEPLKAR 120
           RSETSSELGEPVNSRLK+GKEKKSSKK                     ++F ISEPL+AR
Sbjct: 61  RSETSSELGEPVNSRLKNGKEKKSSKKSKVVESDEDDVEKEADDQNAISKFRISEPLRAR 120

Query: 121 LKEKGITALFPIQAMTFDTVYDGSDLVRQARTGQGKTLAFVLPILESLVNGPSKSSRVIG 180
           LKEKGI++LFPIQAMTFDTVYDG DLV +ARTGQGKTLAFVLPILES+VNGPSKSS+V G
Sbjct: 121 LKEKGISSLFPIQAMTFDTVYDGFDLVGRARTGQGKTLAFVLPILESIVNGPSKSSKVTG 180

Query: 181 YGRSPSVIVLLPTRELADQ----------------------------------------- 240
           YGR+PSVIVLLPTRELADQ                                         
Sbjct: 181 YGRTPSVIVLLPTRELADQVFEDFKFYGHALGLESCCLCGGMLYGPQENKLRRGVDIVVG 240

Query: 241 ------DHINRNNIDIRSLKFRVLDEADEMLRMGFVEDVEFILGKVEDVNKVQTLLFSAT 257
                 DHINRNNID+RSLKFRVLDEADEMLRMGFV+DVEFILGKVEDVNKVQTLLFSAT
Sbjct: 241 TPGRVKDHINRNNIDLRSLKFRVLDEADEMLRMGFVDDVEFILGKVEDVNKVQTLLFSAT 300

[Close]

BLAST of Lcy03g001750.1 vs. TAIR 10
Match: AT5G62190.1 (DEAD box RNA helicase (PRH75) )

HSP 1 Score: 245.0 bits (624), Expect = 7.1e-65
Identity = 159/308 (51.62%), Postives = 199/308 (64.61%), Query Frame = 0


Query: 2   EKKEKKMKNKALVEA--LGSEEAFEQKSDELVLSDKKKKKKSKSDKESMKRKAVEVADDE 61
           +K+EKKMK K  ++   L S++  +++  +L  SD+++ +K KS K+  KRKA E  +DE
Sbjct: 9   KKEEKKMKKKMALDTPELDSKKGKKEQKLKLSDSDEEESEKKKSKKKDKKRKASE-EEDE 68

Query: 62  DRSETSSELGEPVNSRLKSGKE----KKSSKKARFLISEPLKARLKEKGITALFPIQAMT 121
            +S++SSE  +  + ++K G E       +  ++F IS PL+ +LK  GI ALFPIQA T
Sbjct: 69  VKSDSSSE-KKKSSKKVKLGVEDVEVDNPNAVSKFRISAPLREKLKANGIEALFPIQAST 128

Query: 122 FDTVYDGSDLVRQARTGQGKTLAFVLPILESLVNGPSKSSRVIGYGRSPSVIVLLPTREL 181
           FD V DG+DLV +ARTGQGKTLAFVLPILESLVNGP+KS R +GYGRSPSV+VLLPTREL
Sbjct: 129 FDMVLDGADLVGRARTGQGKTLAFVLPILESLVNGPAKSKRKMGYGRSPSVLVLLPTREL 188

Query: 182 ADQ-----------------------------------------------DHINRNNIDI 241
           A Q                                               DHI R N+D 
Sbjct: 189 AKQVAADFDAYGGSLGLSSCCLYGGDSYPVQEGKLKRGVDIVVGTPGRIKDHIERQNLDF 248

Query: 242 RSLKFRVLDEADEMLRMGFVEDVEFILGKVEDVNKVQTLLFSATLPDWVKDIASRFLKAN 257
             L+FRVLDEADEMLRMGFVEDVE ILGKVED  KVQTLLFSATLP WVK+I++RFLK +
Sbjct: 249 SYLQFRVLDEADEMLRMGFVEDVELILGKVEDSTKVQTLLFSATLPSWVKNISNRFLKRD 308

[Close]

BLAST of Lcy03g001750.1 vs. TAIR 10
Match: AT3G22330.1 (putative mitochondrial RNA helicase 2 )

HSP 1 Score: 120.9 bits (302), Expect = 1.5e-27
Identity = 74/210 (35.24%), Postives = 108/210 (51.43%), Query Frame = 0


Query: 92  ISEPLKARLKEKGITALFPIQAMTFDTVYDGSDLVRQARTGQGKTLAFVLPILESLVNGP 151
           IS  +   L  KGI  LFPIQ    +   +G D++ +ARTG GKTLAF +PI++ ++   
Sbjct: 111 ISPEIVKALSSKGIEKLFPIQKAVLEPAMEGRDMIGRARTGTGKTLAFGIPIIDKIIKYN 170

Query: 152 SKSSRVIGYGRSPSVIVLLPTRELADQ--------------------------------- 211
           +K  R    GR+P  +VL PTRELA Q                                 
Sbjct: 171 AKHGR----GRNPLCLVLAPTRELARQVEKEFRESAPSLDTICLYGGTPIGQQMRQLDYG 230

Query: 212 ------------DHINRNNIDIRSLKFRVLDEADEMLRMGFVEDVEFILGKVEDVNKVQT 257
                       D + R  +++  ++F VLDEAD+ML++GF EDVE IL K+ +  K Q+
Sbjct: 231 VDVAVGTPGRVIDLMKRGALNLSEVQFVVLDEADQMLQVGFAEDVEIILEKLPE--KRQS 290

[Close]

BLAST of Lcy03g001750.1 vs. TAIR 10
Match: AT3G22310.1 (putative mitochondrial RNA helicase 1 )

HSP 1 Score: 119.0 bits (297), Expect = 5.9e-27
Identity = 73/210 (34.76%), Postives = 108/210 (51.43%), Query Frame = 0


Query: 92  ISEPLKARLKEKGITALFPIQAMTFDTVYDGSDLVRQARTGQGKTLAFVLPILESLVNGP 151
           IS  +   LK +GI  LFPIQ    +   +G D++ +ARTG GKTLAF +PI++ ++   
Sbjct: 123 ISPEIVKALKGRGIEKLFPIQKAVLEPAMEGRDMIGRARTGTGKTLAFGIPIIDKIIKFN 182

Query: 152 SKSSRVIGYGRSPSVIVLLPTRELADQ--------------------------------- 211
           +K  R    G++P  +VL PTRELA Q                                 
Sbjct: 183 AKHGR----GKNPQCLVLAPTRELARQVEKEFRESAPSLDTICLYGGTPIGQQMRELNYG 242

Query: 212 ------------DHINRNNIDIRSLKFRVLDEADEMLRMGFVEDVEFILGKVEDVNKVQT 257
                       D + R  +++  ++F VLDEAD+ML++GF EDVE IL K+    K Q+
Sbjct: 243 IDVAVGTPGRIIDLMKRGALNLSEVQFVVLDEADQMLQVGFAEDVEIILQKLP--AKRQS 302

[Close]

BLAST of Lcy03g001750.1 vs. TAIR 10
Match: AT5G26742.1 (DEAD box RNA helicase (RH3) )

HSP 1 Score: 95.1 bits (235), Expect = 9.1e-20
Identity = 84/275 (30.55%), Postives = 123/275 (44.73%), Query Frame = 0


Query: 27  SDELVLSDKKKKKKSKSDKESMKRKAVEVADDEDRSETSSELGEPVNSRLKSGKEKKSSK 86
           S  L++        S   +E+ K   +   D+ D        G+  N     G+E   SK
Sbjct: 53  SSSLLVPSAVATPNSVLSEEAFKSLGLSDHDEYDLD------GDNNNVEADDGEELAISK 112

Query: 87  KARFLISEPLKARLKEKGITALFPIQAMTFDTVYDGSDLVRQARTGQGKTLAFVLPILES 146
            +   + + L+  L+++GIT LFPIQ         G D++ +A+TG GKTLAF +PI++ 
Sbjct: 113 LS---LPQRLEESLEKRGITHLFPIQRAVLVPALQGRDIIARAKTGTGKTLAFGIPIIKR 172

Query: 147 LVNGPSKSSRVIGYGRSPSVIVLLPTRELADQ---------------------------- 206
           L       +     GR P  +VL PTRELA Q                            
Sbjct: 173 LTEEAGDYTAFRRSGRLPKFLVLAPTRELAKQVEKEIKESAPYLSTVCVYGGVSYTIQQS 232

Query: 207 -----------------DHINRNNIDIRSLKFRVLDEADEMLRMGFVEDVEFILGKVEDV 257
                            D I   ++ +  +++ VLDEAD+ML +GF E VE IL  +   
Sbjct: 233 ALTRGVDVVVGTPGRIIDLIEGRSLKLGEVEYLVLDEADQMLAVGFEEAVESILENLP-- 292

[Close]

BLAST of Lcy03g001750.1 vs. TAIR 10
Match: AT5G26742.2 (DEAD box RNA helicase (RH3) )

HSP 1 Score: 95.1 bits (235), Expect = 9.1e-20
Identity = 84/275 (30.55%), Postives = 123/275 (44.73%), Query Frame = 0


Query: 27  SDELVLSDKKKKKKSKSDKESMKRKAVEVADDEDRSETSSELGEPVNSRLKSGKEKKSSK 86
           S  L++        S   +E+ K   +   D+ D        G+  N     G+E   SK
Sbjct: 53  SSSLLVPSAVATPNSVLSEEAFKSLGLSDHDEYDLD------GDNNNVEADDGEELAISK 112

Query: 87  KARFLISEPLKARLKEKGITALFPIQAMTFDTVYDGSDLVRQARTGQGKTLAFVLPILES 146
            +   + + L+  L+++GIT LFPIQ         G D++ +A+TG GKTLAF +PI++ 
Sbjct: 113 LS---LPQRLEESLEKRGITHLFPIQRAVLVPALQGRDIIARAKTGTGKTLAFGIPIIKR 172

Query: 147 LVNGPSKSSRVIGYGRSPSVIVLLPTRELADQ---------------------------- 206
           L       +     GR P  +VL PTRELA Q                            
Sbjct: 173 LTEEAGDYTAFRRSGRLPKFLVLAPTRELAKQVEKEIKESAPYLSTVCVYGGVSYTIQQS 232

Query: 207 -----------------DHINRNNIDIRSLKFRVLDEADEMLRMGFVEDVEFILGKVEDV 257
                            D I   ++ +  +++ VLDEAD+ML +GF E VE IL  +   
Sbjct: 233 ALTRGVDVVVGTPGRIIDLIEGRSLKLGEVEYLVLDEADQMLAVGFEEAVESILENLP-- 292

The following BLAST results are available for this feature:

BLAST of Lcy03g001750.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39189	1.0e-63	51.62	DEAD-box ATP-dependent RNA helicase 7 OS=Arabidopsis thaliana OX=3702 GN=RH7 PE=...	[more]
Q650T9	3.4e-56	48.68	DEAD-box ATP-dependent RNA helicase 7 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q41382	7.6e-56	48.89	DEAD-box ATP-dependent RNA helicase 7 OS=Spinacia oleracea OX=3562 GN=RH7 PE=2 S...	[more]
Q9BQ39	6.5e-31	34.21	ATP-dependent RNA helicase DDX50 OS=Homo sapiens OX=9606 GN=DDX50 PE=1 SV=1	[more]
Q99MJ9	3.2e-30	33.69	ATP-dependent RNA helicase DDX50 OS=Mus musculus OX=10090 GN=Ddx50 PE=2 SV=1	[more]

BLAST of Lcy03g001750.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JZR9	3.5e-104	72.24	RNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111491171 PE=3 SV=1	[more]
A0A6J1H389	3.5e-104	72.24	RNA helicase OS=Cucurbita moschata OX=3662 GN=LOC111459276 PE=3 SV=1	[more]
A0A6J1ESS9	4.6e-104	70.68	RNA helicase OS=Cucurbita moschata OX=3662 GN=LOC111435671 PE=3 SV=1	[more]
A0A6J1I4T2	1.0e-103	70.37	RNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111469154 PE=3 SV=1	[more]
A0A1S3CTB6	3.6e-101	69.75	RNA helicase OS=Cucumis melo OX=3656 GN=LOC103504655 PE=3 SV=1	[more]

BLAST of Lcy03g001750.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023551208.1	1.9e-104	70.99	DEAD-box ATP-dependent RNA helicase 7-like [Cucurbita pepo subsp. pepo]	[more]
KAG6579664.1	1.9e-104	70.99	DEAD-box ATP-dependent RNA helicase 7, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022957864.1	7.3e-104	72.24	DEAD-box ATP-dependent RNA helicase 7-like [Cucurbita moschata] >KAG6605682.1 DE...	[more]
XP_022995722.1	7.3e-104	72.24	DEAD-box ATP-dependent RNA helicase 7-like [Cucurbita maxima]	[more]
XP_022928900.1	9.5e-104	70.68	DEAD-box ATP-dependent RNA helicase 7-like [Cucurbita moschata]	[more]

BLAST of Lcy03g001750.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62190.1	7.1e-65	51.62	DEAD box RNA helicase (PRH75)	[more]
AT3G22330.1	1.5e-27	35.24	putative mitochondrial RNA helicase 2	[more]
AT3G22310.1	5.9e-27	34.76	putative mitochondrial RNA helicase 1	[more]
AT5G26742.1	9.1e-20	30.55	DEAD box RNA helicase (RH3)	[more]
AT5G26742.2	9.1e-20	30.55	DEAD box RNA helicase (RH3)	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014001	Helicase superfamily 1/2, ATP-binding domain	SMART	SM00487	ultradead3	coord: 104..261 e-value: 2.2E-13 score: 60.5
IPR014001	Helicase superfamily 1/2, ATP-binding domain	PROSITE	PS51192	HELICASE_ATP_BIND_1	coord: 162..251 score: 12.194107
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 179..253 e-value: 5.6E-18 score: 66.9
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 22..178 e-value: 3.9E-25 score: 90.4
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 89..246
IPR011545	DEAD/DEAH box helicase domain	PFAM	PF00270	DEAD	coord: 109..178 e-value: 6.2E-13 score: 48.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 21..59
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..84
None	No IPR available	PANTHER	PTHR47958	ATP-DEPENDENT RNA HELICASE DBP3	coord: 12..178
None	No IPR available	PANTHER	PTHR47958:SF24	DEAD (ASP-GLU-ALA-ASP) BOX HELICASE 21	coord: 12..178 coord: 178..256
None	No IPR available	PANTHER	PTHR47958	ATP-DEPENDENT RNA HELICASE DBP3	coord: 178..256
None	No IPR available	CDD	cd00268	DEADc	coord: 96..246 e-value: 3.89818E-54 score: 171.856
IPR000629	ATP-dependent RNA helicase DEAD-box, conserved site	PROSITE	PS00039	DEAD_ATP_HELICASE	coord: 195..203

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lcy03g001750	Lcy03g001750	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy03g001750.1.exon1	Lcy03g001750.1.exon1	exon
Lcy03g001750.1.exon2	Lcy03g001750.1.exon2	exon
Lcy03g001750.1.exon3	Lcy03g001750.1.exon3	exon
Lcy03g001750.1.exon4	Lcy03g001750.1.exon4	exon
Lcy03g001750.1.exon5	Lcy03g001750.1.exon5	exon
Lcy03g001750.1.exon6	Lcy03g001750.1.exon6	exon
Lcy03g001750.1.exon7	Lcy03g001750.1.exon7	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy03g001750.1.cds1	Lcy03g001750.1.cds1	CDS
Lcy03g001750.1.cds2	Lcy03g001750.1.cds2	CDS
Lcy03g001750.1.cds3	Lcy03g001750.1.cds3	CDS
Lcy03g001750.1.cds4	Lcy03g001750.1.cds4	CDS
Lcy03g001750.1.cds5	Lcy03g001750.1.cds5	CDS
Lcy03g001750.1.cds6	Lcy03g001750.1.cds6	CDS
Lcy03g001750.1.cds7	Lcy03g001750.1.cds7	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lcy03g001750.1	Lcy03g001750.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005730	nucleolus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003724	RNA helicase activity