Cucsat.G21442.T2 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G21442.T2
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Mannan endo-1,4-beta-mannosidase
Location	ctg910: 3382575 .. 3385536 (+)
RNA-Seq Expression	Cucsat.G21442.T2
Synteny	Cucsat.G21442.T2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCCTTTGTAGAAAAACCCAATGGAAATCACTACTCAATTCGGCTTTGTGGTTTTAGCTTTCATCTCTTTCTTTTCATCTCTTTCTTATTCTTTGCCTCTTTCGACAAACGGGAGATGGATTGTCGACTCCGCCACCGGGCGTCGAGTGAAGCTTGTGTGCGTGAATTGGCCTTCTCACACGCAAAGCATGTTAATCGAAGGCCTTGATCGCAGACCATTGAAAGACCTAGCAAATGAGGTAGTGCGATTGAGGTTTAATTGCGTGAGGCTCACATATGCAACTCACATGTTTACTCGATATGCTAATAGGACAGTTGAAGAGAATTTTGACCTTCTTGATTTGAGAGCTGCTAAGGTGGGGTTGGCTTTGCATAATCCGTTTGTATTGAATATGACCATTTTTGAAGCTTATGAAGCTGTGGTTGATGTGCTCGGGACCAGTGGTTTGATGGTTATAGCTGACAATCATATTAGCCAACCAAGATGGTGTTGCTCTCTTGAGGATGGAAATGGTTTCTTTGGAGATCGATATTTCGACACGGAAGAATGGTTGGAAGGTCTTCGTTTGGTTGCCAGACGATTCTACAACAAATCAGCAGTACGTAAATTCAATCTTCATACTTTTGAAATGATTTTTGTGAAATATGAAACAAATACATAACATAGACACATACACATTTTTATAAAAGTAAGTTACAAGTATAGTTCATTTGTTAAATTAAAGATCAATCCAAATATTTATTAAAATAAGTTAGGGAGGACTCGTGGGTACTCAGTTTGAGTTGTATTAAAGCACTTTTTAGACCCAACTCTATTCAACCATAAAATATATTTACGCTGCTTTGGCTCAGGTTCCTCGAGTCACTCAAGTCATCTATTCATCAAATAATTGGGTTCTTAATATATATTTAGAAAATGAAAATAGTACTAGAAAGTAATTAATTAGTAAGTAAAATTATGTCTTTCTTTGATAAATTTATGGGACTTTCACCTTCACGTCCAAGCAGGTGGTAGCCATGAGCCTACGTAATGAACTCCGAGGAGCAAGCTCAAAATCGAAAGATTGGAACAAGTACATAACACAAGGAGCAACAACAATCCACAACATAAACCCAAAGATCTTGGTAATCATTTCCGGTCTAAACTTCGACAACGATCTAAGATGCCAAAGGCAATACCCCTTGCAACTAAACAACCTACACAACAAGCTAGTATTTGAAGTACACTTATATTCCTTTAGTGGAGAATCCCAATCAAAGTTCATCCATAACCCACTCAACAAAATTTGCTCAAAGGTCATCAATGGGTTTGTGGAGAGAGCTGAGTTTGTGATGGAAGGAGCTGAGGCAGTTCCTTTGTTTGTGAGTGAATTTGGGTTGGACCAAAGGGGAGTTAATGAGGCTGATGATAGGTTTTTGAGTTGCTTTAGTGCTCATCTTGTTGAGAAAGATTTGGATTGGGCATTATGGGGTTGGCAAGGGAGCTATTATTATAGACAGGGTAAAGTAGGACCTGAAGAAGTGTTTGGAGTTTTGAATTACAATTGGAGTGATGTTAGAAACCCTCATTTTTCTCAGATGTTTCAACTCTTGCAAACCATGTTACAAGGTATGATTTCAAGTTCTATTTGATGACGCTTTTGTTTCGGTTTTTTTATTTTATAAAACGACTAAGTTCATTTTTCACTATACATAAATGATAAACTACAAAGGACAAAATGAAATTTTAAAAATGAACTTTTGAATAGAACATACGGTTAATGATAGTCTATTTTCTCTTGTTACTTTGTCTATCGAAAATACTCTTATACTTTCAAAAGTTTTTTAAAAAATTTAAACTCCTAAACAAGAGTTCAAAAATACTTTTGTCATTAGCTTTTCATAGAAACGGTTAGTATTTTATTTCAGAAATACTCATAAATTGTCAAAATTAATATCAACAAAGCTCCTTTAAGCTATAATAAAAAGAAAATAAAAAGTTGTTAAAAAAATACTCTTACATCCGCATATGAACAAAATCCATTAACATTTGTGAATCATATTAATATTAAATTTTTAATGATTTTACAACTCAACTTTTTTATGATTCGATAATAGCAATTGTGGTAGTACTTTTAGAGCTAATAATTAAGTGTATAGGAACACTTCAAAAGAATTACGAATATAATAAAATCCATCAATGATAGATATTATCGCTGATAGACTCCTAATAATAAAGTCTATTACTTATAGGCTTCGAGAGTTGGACTTAAATTTTACTATATCTATACATTTCTTTTATATTATGATATATTTGAAAATATTTTGGATATAATCTCTATATTTGTAACTAGCTTAATTTGATTTATAGATCCAAATTCCAATTCCTCAAACACTTACGTGATGTATCATCCACAAAGTGGGCAATGTGTCCTAGTGCAAGACATGAAACATATGCAAATTTATCTCAACGATTGTTCAAATGCAAGCCATTGGAGTTATGAAGGAGATGGAACTCCAATAATGTTGGCATCCACTAATTTTTGTCTAAAAGCCAGTGGAGATGGGCTTCCACCATCACTCTCAAGGGATTGTTTTGGTGAACAAAGTGTTTGGACAGCCATTTCAGATTCTAAGCTTCATTTGGCCACACTAACAAAGCAAGGCAATAATGGTATGTGTTTAGAGAAAGAGAGCTCAAATTCTAGTAGGATTTTGATGAGGAGTTGCGTTTGTGTTGGTAATGATTCAAATTGTTTACAAGATACTCAAGCTCAATGGTTTCAACTTGTTGTTACAAATACTTTGTAAATTGTTCACATGGGGAGATGAAATGGTGAAATAAGAAAGAGGGATTATTTAGTTTCAATTATTTGTTTCATGAGGACCTTTTGCCTTTTCTCTTCAAGTATTTTAACGTATACATATCTTAGGATGATTTTGAGTTTGATTTTTAAGTAAATTTTGTCGTCTTTGTTCGAGT

Coding sequence (CDS)

ATGAGCCTACGTAATGAACTCCGAGGAGCAAGCTCAAAATCGAAAGATTGGAACAAGTACATAACACAAGGAGCAACAACAATCCACAACATAAACCCAAAGATCTTGGTAATCATTTCCGGTCTAAACTTCGACAACGATCTAAGATGCCAAAGGCAATACCCCTTGCAACTAAACAACCTACACAACAAGCTAGTATTTGAAGTACACTTATATTCCTTTAGTGGAGAATCCCAATCAAAGTTCATCCATAACCCACTCAACAAAATTTGCTCAAAGGTCATCAATGGGTTTGTGGAGAGAGCTGAGTTTGTGATGGAAGGAGCTGAGGCAGTTCCTTTGTTTGTGAGTGAATTTGGGTTGGACCAAAGGGGAGTTAATGAGGCTGATGATAGGTTTTTGAGTTGCTTTAGTGCTCATCTTGTTGAGAAAGATTTGGATTGGGCATTATGGGGTTGGCAAGGGAGCTATTATTATAGACAGGGTAAAGTAGGACCTGAAGAAGTGTTTGGAGTTTTGAATTACAATTGGAGTGATGTTAGAAACCCTCATTTTTCTCAGATGTTTCAACTCTTGCAAACCATGTTACAAGATCCAAATTCCAATTCCTCAAACACTTACGTGATGTATCATCCACAAAGTGGGCAATGTGTCCTAGTGCAAGACATGAAACATATGCAAATTTATCTCAACGATTGTTCAAATGCAAGCCATTGGAGTTATGAAGGAGATGGAACTCCAATAATGTTGGCATCCACTAATTTTTGTCTAAAAGCCAGTGGAGATGGGCTTCCACCATCACTCTCAAGGGATTGTTTTGGTGAACAAAGTGTTTGGACAGCCATTTCAGATTCTAAGCTTCATTTGGCCACACTAACAAAGCAAGGCAATAATGGTATGTGTTTAGAGAAAGAGAGCTCAAATTCTAGTAGGATTTTGATGAGGAGTTGCGTTTGTGTTGGTAATGATTCAAATTGTTTACAAGATACTCAAGCTCAATGGTTTCAACTTGTTGTTACAAATACTTTGTAA

Protein sequence

MSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAEAVPLFVSEFGLDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPEEVFGVLNYNWSDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVLVQDMKHMQIYLNDCSNASHWSYEGDGTPIMLASTNFCLKASGDGLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGMCLEKESSNSSRILMRSCVCVGNDSNCLQDTQAQWFQLVVTNTL

Homology

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BLAST of Cucsat.G21442.T2 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 9.3e-59
Identity = 137/355 (38.59%), Postives = 195/355 (54.93%), Query Frame = 0


Query: 1   MSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQRQYPLQLNN 60
           MSLRNELRG  S    W++++  GA T+H  NPK+LVI+SGL FD DL      P+ L  
Sbjct: 203 MSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSFLPVLPVTL-P 262

Query: 61  LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAE--AVPLFVSE 120
              K+V+E H YSF    ++       N +C      F     FV   A   A PLF+SE
Sbjct: 263 FKEKIVYEGHWYSFGVPWRTGL----PNDVCKNETGRFKSNVGFVTSSANATAAPLFMSE 322

Query: 121 FGLDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGP---EEVFGVLNY 180
           FG+DQR VN+ D+R+L+C  A+L E+DLDWALW   GSYYYR  K      EE +G  N+
Sbjct: 323 FGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQPVKDFEETYGFFNH 382

Query: 181 NWSDVRNPHFSQMFQLLQTMLQDPNSNSSNTY-VMYHPQSGQCVLVQDMKHMQIYLNDCS 240
           +WS +RNP F    + +Q  +QDP       Y ++YHP SG C  V+      ++L  C 
Sbjct: 383 DWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLC--VESGIGNTVHLGSCQ 442

Query: 241 NA-SHWSYEGD-GTPIMLASTNFCLKASGDGLPPSLSRDCFG-EQSVWTAISDSKLHLAT 300
           +  S W+Y+     PI L  ++ C+   G+GLP  ++ +C     ++W+ +S ++L L T
Sbjct: 443 SVRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWSTVSSAQLQLGT 502

Query: 301 --LTKQG-NNGMCLEKESSNSSRILMRSCVCVGNDSNCLQ--DTQAQWFQLVVTN 342
             L K G    MCL  + S S  I    C+C+  DS+C    + + QWF+++ TN
Sbjct: 503 RVLGKDGKEKWMCL--DGSKSPLISTNECICI-TDSHCYPKLNPEKQWFKVITTN 547

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BLAST of Cucsat.G21442.T2 vs. NCBI nr
Match: XP_004143723.1 (glycosyl hydrolase 5 family protein [Cucumis sativus] >KAE8647936.1 hypothetical protein Csa_000506 [Cucumis sativus])

HSP 1 Score: 715 bits (1846), Expect = 2.33e-257
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0


Query: 1   MSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQRQYPLQLNN 60
           MSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQRQYPLQLNN
Sbjct: 197 MSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQRQYPLQLNN 256

Query: 61  LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAEAVPLFVSEFG 120
           LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAEAVPLFVSEFG
Sbjct: 257 LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAEAVPLFVSEFG 316

Query: 121 LDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPEEVFGVLNYNWSDV 180
           LDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPEEVFGVLNYNWSDV
Sbjct: 317 LDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPEEVFGVLNYNWSDV 376

Query: 181 RNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVLVQDMKHMQIYLNDCSNASHWS 240
           RNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVLVQDMKHMQIYLNDCSNASHWS
Sbjct: 377 RNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVLVQDMKHMQIYLNDCSNASHWS 436

Query: 241 YEGDGTPIMLASTNFCLKASGDGLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGM 300
           YEGDGTPIMLASTNFCLKASGDGLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGM
Sbjct: 437 YEGDGTPIMLASTNFCLKASGDGLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGM 496

Query: 301 CLEKESSNSSRILMRSCVCVGNDSNCLQDTQAQWFQLVVTNTL 343
           CLEKESSNSSRILMRSCVCVGNDSNCLQDTQAQWFQLVVTNTL
Sbjct: 497 CLEKESSNSSRILMRSCVCVGNDSNCLQDTQAQWFQLVVTNTL 539

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BLAST of Cucsat.G21442.T2 vs. NCBI nr
Match: XP_008467306.1 (PREDICTED: major extracellular endoglucanase-like [Cucumis melo])

HSP 1 Score: 679 bits (1751), Expect = 6.71e-243
Identity = 324/343 (94.46%), Postives = 334/343 (97.38%), Query Frame = 0


Query: 1   MSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQRQYPLQLNN 60
           MSLRNELRGASSKSKDWNKY+TQGATTIHNINP ILVIISGLNFDNDLRCQRQYPLQLNN
Sbjct: 197 MSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNFDNDLRCQRQYPLQLNN 256

Query: 61  LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAEAVPLFVSEFG 120
           LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSK+INGFV+RAEFVMEGAEAVPLFVSEFG
Sbjct: 257 LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEFVMEGAEAVPLFVSEFG 316

Query: 121 LDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPEEVFGVLNYNWSDV 180
           LDQ GVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV  EEVFGVLNYNWSDV
Sbjct: 317 LDQTGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVELEEVFGVLNYNWSDV 376

Query: 181 RNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVLVQDMKHMQIYLNDCSNASHWS 240
           RNP FSQMFQLLQTMLQDPNSNSSNTY+MYHPQSGQCV V DMK  +I+LN+CSNASHWS
Sbjct: 377 RNPRFSQMFQLLQTMLQDPNSNSSNTYLMYHPQSGQCVQVHDMKQKEIFLNNCSNASHWS 436

Query: 241 YEGDGTPIMLASTNFCLKASGDGLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGM 300
           YEGDGTPIMLASTNFCLKA+G+GLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGM
Sbjct: 437 YEGDGTPIMLASTNFCLKANGNGLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGM 496

Query: 301 CLEKESSNSSRILMRSCVCVGNDSNCLQDTQAQWFQLVVTNTL 343
           CLEKESSNSSRILMRSCVCVG+DSNCLQDTQAQWFQLVVTNTL
Sbjct: 497 CLEKESSNSSRILMRSCVCVGSDSNCLQDTQAQWFQLVVTNTL 539

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BLAST of Cucsat.G21442.T2 vs. NCBI nr
Match: KAA0055112.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 676 bits (1744), Expect = 7.80e-242
Identity = 322/343 (93.88%), Postives = 334/343 (97.38%), Query Frame = 0


Query: 1   MSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQRQYPLQLNN 60
           MSLRNELRGASSKSKDWNKY+TQGATTIHNINP ILVIISGLNFDNDLRCQRQYPLQLNN
Sbjct: 197 MSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNFDNDLRCQRQYPLQLNN 256

Query: 61  LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAEAVPLFVSEFG 120
           LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSK+INGFV+RAEFVMEGAEAVPLFVSEFG
Sbjct: 257 LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEFVMEGAEAVPLFVSEFG 316

Query: 121 LDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPEEVFGVLNYNWSDV 180
           LDQ GVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV  EEVFGVLNYNWSDV
Sbjct: 317 LDQTGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVELEEVFGVLNYNWSDV 376

Query: 181 RNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVLVQDMKHMQIYLNDCSNASHWS 240
           RNP FSQMFQLLQTMLQDPNSNSSNTY+MYHPQSGQCV V DMK  +I+L++CSNASHWS
Sbjct: 377 RNPRFSQMFQLLQTMLQDPNSNSSNTYLMYHPQSGQCVQVHDMKKKEIFLDNCSNASHWS 436

Query: 241 YEGDGTPIMLASTNFCLKASGDGLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGM 300
           YEGDGTPIMLASTNFCLKA+G+GLPPSLSRDCFGEQS+WTAISDSKLHLATLTKQGNNGM
Sbjct: 437 YEGDGTPIMLASTNFCLKANGNGLPPSLSRDCFGEQSIWTAISDSKLHLATLTKQGNNGM 496

Query: 301 CLEKESSNSSRILMRSCVCVGNDSNCLQDTQAQWFQLVVTNTL 343
           CLEKESSNSSRILMRSCVCVG+DSNCLQDTQAQWFQLVVTNTL
Sbjct: 497 CLEKESSNSSRILMRSCVCVGSDSNCLQDTQAQWFQLVVTNTL 539

[Close]

BLAST of Cucsat.G21442.T2 vs. NCBI nr
Match: XP_038875504.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 651 bits (1679), Expect = 5.63e-232
Identity = 307/342 (89.77%), Postives = 324/342 (94.74%), Query Frame = 0


Query: 1   MSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQRQYPLQLNN 60
           MSLRNELRG  SKSKDWNKY+TQGATTIH INP IL+IISGLNFDNDLRCQRQ PL LNN
Sbjct: 195 MSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNFDNDLRCQRQNPLPLNN 254

Query: 61  LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAEAVPLFVSEFG 120
           LHNKLVFEVHLYSFSGESQSKFI+NPLNKICSK+INGFVERAEFVMEGAEAVPLFVSEFG
Sbjct: 255 LHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEFVMEGAEAVPLFVSEFG 314

Query: 121 LDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPEEVFGVLNYNWSDV 180
            DQRGVNEADDRFLSCF AHL +KDLDWALW WQGSYYYRQGK+ PEEVFGVLNYNW+DV
Sbjct: 315 FDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKIEPEEVFGVLNYNWTDV 374

Query: 181 RNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVLVQDMKHMQIYLNDCSNASHWS 240
           RNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCV V+DM   QIYLN+CSNASHWS
Sbjct: 375 RNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKDMMDKQIYLNNCSNASHWS 434

Query: 241 YEGDGTPIMLASTNFCLKASGDGLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGM 300
           +EGDGTPIML +TNFCLKA+G+GLPPSLS DCFGEQSVWTAISDSKLHLATLTKQGNNG+
Sbjct: 435 HEGDGTPIMLEATNFCLKANGNGLPPSLSSDCFGEQSVWTAISDSKLHLATLTKQGNNGL 494

Query: 301 CLEKESSNSSRILMRSCVCVGNDSNCLQDTQAQWFQLVVTNT 342
           CLEKESSNS+RI+M SCVCVGNDSNCLQDTQAQWF+LVVTNT
Sbjct: 495 CLEKESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFELVVTNT 536

[Close]

BLAST of Cucsat.G21442.T2 vs. NCBI nr
Match: XP_023534098.1 (uncharacterized protein LOC111795760 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 570 bits (1469), Expect = 4.77e-200
Identity = 269/343 (78.43%), Postives = 299/343 (87.17%), Query Frame = 0


Query: 1   MSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQRQYPLQLNN 60
           MSLRNELRGA S SKDWNKY+TQGATTIH+INP +LVI+SGLNFDNDLRCQR  PL LNN
Sbjct: 196 MSLRNELRGAKSSSKDWNKYMTQGATTIHDINPNLLVIVSGLNFDNDLRCQRHNPLLLNN 255

Query: 61  LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAEAVPLFVSEFG 120
           LHNKLVFEVHLYSFSG +++KFI NPLNKICS +INGFVERAEFVM+GAEAVPLFVSEFG
Sbjct: 256 LHNKLVFEVHLYSFSGATKTKFITNPLNKICSTIINGFVERAEFVMQGAEAVPLFVSEFG 315

Query: 121 LDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPEEVFGVLNYNWSDV 180
            DQRG N ADDRFLSCF AHL + DLDWALW WQGSYYYRQG+   +EVFGVLNYNWSDV
Sbjct: 316 FDQRGTNVADDRFLSCFVAHLAKTDLDWALWAWQGSYYYRQGQAQSDEVFGVLNYNWSDV 375

Query: 181 RNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVLVQDMKHMQIYLNDCSNASHWS 240
           RNP FS+ FQLLQTML+DPNSN+ N+YVMYHPQSGQCV V+DM   +IYLNDCSNASHWS
Sbjct: 376 RNPRFSKTFQLLQTMLRDPNSNAPNSYVMYHPQSGQCVRVKDMMSKEIYLNDCSNASHWS 435

Query: 241 YEGDGTPIMLASTNFCLKASGDGLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGM 300
           + GDGTPI L +T  CLKA GDGL P LSRDC   +S WT IS+SKLHLATLT+QGN G+
Sbjct: 436 HRGDGTPIELEATGLCLKADGDGLRPLLSRDCSSNESSWTTISNSKLHLATLTRQGN-GL 495

Query: 301 CLEKESSNSSRILMRSCVCVGNDSNCLQDTQAQWFQLVVTNTL 343
           CLEKESSNS+RI+M  CVCVG+DSNCL DT++QWF+LV TNTL
Sbjct: 496 CLEKESSNSTRIVMGRCVCVGDDSNCLDDTRSQWFELVATNTL 537

[Close]

BLAST of Cucsat.G21442.T2 vs. ExPASy TrEMBL
Match: A0A0A0KKZ0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G168960 PE=3 SV=1)

HSP 1 Score: 715 bits (1846), Expect = 7.81e-261
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0


Query: 1   MSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQRQYPLQLNN 60
           MSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQRQYPLQLNN
Sbjct: 1   MSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQRQYPLQLNN 60

Query: 61  LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAEAVPLFVSEFG 120
           LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAEAVPLFVSEFG
Sbjct: 61  LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAEAVPLFVSEFG 120

Query: 121 LDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPEEVFGVLNYNWSDV 180
           LDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPEEVFGVLNYNWSDV
Sbjct: 121 LDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPEEVFGVLNYNWSDV 180

Query: 181 RNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVLVQDMKHMQIYLNDCSNASHWS 240
           RNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVLVQDMKHMQIYLNDCSNASHWS
Sbjct: 181 RNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVLVQDMKHMQIYLNDCSNASHWS 240

Query: 241 YEGDGTPIMLASTNFCLKASGDGLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGM 300
           YEGDGTPIMLASTNFCLKASGDGLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGM
Sbjct: 241 YEGDGTPIMLASTNFCLKASGDGLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGM 300

Query: 301 CLEKESSNSSRILMRSCVCVGNDSNCLQDTQAQWFQLVVTNTL 343
           CLEKESSNSSRILMRSCVCVGNDSNCLQDTQAQWFQLVVTNTL
Sbjct: 301 CLEKESSNSSRILMRSCVCVGNDSNCLQDTQAQWFQLVVTNTL 343

[Close]

BLAST of Cucsat.G21442.T2 vs. ExPASy TrEMBL
Match: A0A1S3CTF8 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103504686 PE=3 SV=1)

HSP 1 Score: 679 bits (1751), Expect = 3.25e-243
Identity = 324/343 (94.46%), Postives = 334/343 (97.38%), Query Frame = 0


Query: 1   MSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQRQYPLQLNN 60
           MSLRNELRGASSKSKDWNKY+TQGATTIHNINP ILVIISGLNFDNDLRCQRQYPLQLNN
Sbjct: 197 MSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNFDNDLRCQRQYPLQLNN 256

Query: 61  LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAEAVPLFVSEFG 120
           LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSK+INGFV+RAEFVMEGAEAVPLFVSEFG
Sbjct: 257 LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEFVMEGAEAVPLFVSEFG 316

Query: 121 LDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPEEVFGVLNYNWSDV 180
           LDQ GVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV  EEVFGVLNYNWSDV
Sbjct: 317 LDQTGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVELEEVFGVLNYNWSDV 376

Query: 181 RNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVLVQDMKHMQIYLNDCSNASHWS 240
           RNP FSQMFQLLQTMLQDPNSNSSNTY+MYHPQSGQCV V DMK  +I+LN+CSNASHWS
Sbjct: 377 RNPRFSQMFQLLQTMLQDPNSNSSNTYLMYHPQSGQCVQVHDMKQKEIFLNNCSNASHWS 436

Query: 241 YEGDGTPIMLASTNFCLKASGDGLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGM 300
           YEGDGTPIMLASTNFCLKA+G+GLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGM
Sbjct: 437 YEGDGTPIMLASTNFCLKANGNGLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGM 496

Query: 301 CLEKESSNSSRILMRSCVCVGNDSNCLQDTQAQWFQLVVTNTL 343
           CLEKESSNSSRILMRSCVCVG+DSNCLQDTQAQWFQLVVTNTL
Sbjct: 497 CLEKESSNSSRILMRSCVCVGSDSNCLQDTQAQWFQLVVTNTL 539

[Close]

BLAST of Cucsat.G21442.T2 vs. ExPASy TrEMBL
Match: A0A5A7UH07 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00380 PE=3 SV=1)

HSP 1 Score: 676 bits (1744), Expect = 3.78e-242
Identity = 322/343 (93.88%), Postives = 334/343 (97.38%), Query Frame = 0


Query: 1   MSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQRQYPLQLNN 60
           MSLRNELRGASSKSKDWNKY+TQGATTIHNINP ILVIISGLNFDNDLRCQRQYPLQLNN
Sbjct: 197 MSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNFDNDLRCQRQYPLQLNN 256

Query: 61  LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAEAVPLFVSEFG 120
           LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSK+INGFV+RAEFVMEGAEAVPLFVSEFG
Sbjct: 257 LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEFVMEGAEAVPLFVSEFG 316

Query: 121 LDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPEEVFGVLNYNWSDV 180
           LDQ GVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV  EEVFGVLNYNWSDV
Sbjct: 317 LDQTGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVELEEVFGVLNYNWSDV 376

Query: 181 RNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVLVQDMKHMQIYLNDCSNASHWS 240
           RNP FSQMFQLLQTMLQDPNSNSSNTY+MYHPQSGQCV V DMK  +I+L++CSNASHWS
Sbjct: 377 RNPRFSQMFQLLQTMLQDPNSNSSNTYLMYHPQSGQCVQVHDMKKKEIFLDNCSNASHWS 436

Query: 241 YEGDGTPIMLASTNFCLKASGDGLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGM 300
           YEGDGTPIMLASTNFCLKA+G+GLPPSLSRDCFGEQS+WTAISDSKLHLATLTKQGNNGM
Sbjct: 437 YEGDGTPIMLASTNFCLKANGNGLPPSLSRDCFGEQSIWTAISDSKLHLATLTKQGNNGM 496

Query: 301 CLEKESSNSSRILMRSCVCVGNDSNCLQDTQAQWFQLVVTNTL 343
           CLEKESSNSSRILMRSCVCVG+DSNCLQDTQAQWFQLVVTNTL
Sbjct: 497 CLEKESSNSSRILMRSCVCVGSDSNCLQDTQAQWFQLVVTNTL 539

[Close]

BLAST of Cucsat.G21442.T2 vs. ExPASy TrEMBL
Match: A0A6J1K1F7 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111490847 PE=3 SV=1)

HSP 1 Score: 567 bits (1462), Expect = 2.67e-199
Identity = 267/343 (77.84%), Postives = 298/343 (86.88%), Query Frame = 0


Query: 1   MSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQRQYPLQLNN 60
           MSLRNELRGA S SKDWNKY+TQGATTIH+INP +LVI+SGLNFDNDLRCQR  PL LNN
Sbjct: 196 MSLRNELRGAKSSSKDWNKYMTQGATTIHDINPNLLVIVSGLNFDNDLRCQRHNPLPLNN 255

Query: 61  LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAEAVPLFVSEFG 120
           LHNKLVFEVHLYSFSG +++KFI NPLNKICS +INGFVERAEFVM+GAEAVPLFVSEFG
Sbjct: 256 LHNKLVFEVHLYSFSGATKTKFITNPLNKICSAIINGFVERAEFVMQGAEAVPLFVSEFG 315

Query: 121 LDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPEEVFGVLNYNWSDV 180
            DQRG N ADDRF SCF AHL   DLDWALW WQGSYYYRQG+   +EVFGVLNYNWSDV
Sbjct: 316 FDQRGTNVADDRFSSCFVAHLARTDLDWALWAWQGSYYYRQGQAQSDEVFGVLNYNWSDV 375

Query: 181 RNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVLVQDMKHMQIYLNDCSNASHWS 240
           RNPHFS+ FQLLQTML+DPNSN+ N+YVMYHPQSGQCV V+DM    IYLNDCSNASHWS
Sbjct: 376 RNPHFSKTFQLLQTMLRDPNSNAPNSYVMYHPQSGQCVRVKDMMSKDIYLNDCSNASHWS 435

Query: 241 YEGDGTPIMLASTNFCLKASGDGLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGM 300
           + GDGTPI L +T+ CLKA  DGL P LSRDC G++S WT IS+SKLHLAT T+QGN G+
Sbjct: 436 HRGDGTPIELEATSLCLKADRDGLRPLLSRDCSGDESAWTTISNSKLHLATFTRQGN-GL 495

Query: 301 CLEKESSNSSRILMRSCVCVGNDSNCLQDTQAQWFQLVVTNTL 343
           CLEKESSNS+RI+M  C+CVG+DSNCL DT++QWF+LV TNTL
Sbjct: 496 CLEKESSNSTRIVMGRCLCVGDDSNCLDDTRSQWFELVGTNTL 537

[Close]

BLAST of Cucsat.G21442.T2 vs. ExPASy TrEMBL
Match: A0A6J1H201 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459707 PE=3 SV=1)

HSP 1 Score: 565 bits (1457), Expect = 1.54e-198
Identity = 266/343 (77.55%), Postives = 298/343 (86.88%), Query Frame = 0


Query: 1   MSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQRQYPLQLNN 60
           MSLRNELRGA S SKDWNKY+TQGATTIH+INP +LVI+SGLNFDNDLRCQR  PL LNN
Sbjct: 196 MSLRNELRGAKSSSKDWNKYMTQGATTIHDINPNLLVIVSGLNFDNDLRCQRHNPLPLNN 255

Query: 61  LHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAEAVPLFVSEFG 120
           LHNKLVFEVHLYSFSG +++KFI NPLNKICS +INGFVERAEFVM+GAEAVPLFVSEFG
Sbjct: 256 LHNKLVFEVHLYSFSGATKTKFITNPLNKICSTIINGFVERAEFVMQGAEAVPLFVSEFG 315

Query: 121 LDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPEEVFGVLNYNWSDV 180
            DQRG N ADDRFLSCF AHL + DLDWALW WQGSYYYRQG+   +EVFG+LNYNWS V
Sbjct: 316 FDQRGTNVADDRFLSCFVAHLAKTDLDWALWAWQGSYYYRQGQAQFDEVFGILNYNWSGV 375

Query: 181 RNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVLVQDMKHMQIYLNDCSNASHWS 240
           RNP FS+ FQLLQTML+DPNSN+ N+YVMYHPQSGQCV V+DM   +IYLNDCSNASHWS
Sbjct: 376 RNPRFSKTFQLLQTMLRDPNSNAPNSYVMYHPQSGQCVRVKDMMSKEIYLNDCSNASHWS 435

Query: 241 YEGDGTPIMLASTNFCLKASGDGLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGM 300
           + GDGTPI L +T  CLKA GDGL P LSRDC   +S WT IS+SKLHLATLT+ GN G+
Sbjct: 436 HRGDGTPIELEATGLCLKADGDGLRPLLSRDCLSNESSWTTISNSKLHLATLTRHGN-GL 495

Query: 301 CLEKESSNSSRILMRSCVCVGNDSNCLQDTQAQWFQLVVTNTL 343
           CLEK+SSNS+RI+M  CVCVG+DSNCL DT++QWF+LVVTNTL
Sbjct: 496 CLEKDSSNSTRIVMGRCVCVGDDSNCLDDTRSQWFELVVTNTL 537

The following BLAST results are available for this feature:

BLAST of Cucsat.G21442.T2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0HLA0	9.3e-59	38.59	Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]

BLAST of Cucsat.G21442.T2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143723.1	2.33e-257	100.00	glycosyl hydrolase 5 family protein [Cucumis sativus] >KAE8647936.1 hypothetical...	[more]
XP_008467306.1	6.71e-243	94.46	PREDICTED: major extracellular endoglucanase-like [Cucumis melo]	[more]
KAA0055112.1	7.80e-242	93.88	major extracellular endoglucanase-like [Cucumis melo var. makuwa]	[more]
XP_038875504.1	5.63e-232	89.77	glycosyl hydrolase 5 family protein-like [Benincasa hispida]	[more]
XP_023534098.1	4.77e-200	78.43	uncharacterized protein LOC111795760 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G21442.T2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KKZ0	7.81e-261	100.00	Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G168960 PE=3...	[more]
A0A1S3CTF8	3.25e-243	94.46	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103504686 PE=3 SV...	[more]
A0A5A7UH07	3.78e-242	93.88	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A6J1K1F7	2.67e-199	77.84	Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111490847 PE=...	[more]
A0A6J1H201	1.54e-198	77.55	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459707 P...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 1..193 e-value: 2.3E-30 score: 107.9
None	No IPR available	GENE3D	2.80.10.50		coord: 211..325 e-value: 3.9E-5 score: 25.6
None	No IPR available	PANTHER	PTHR31263	CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)	coord: 1..339
IPR001547	Glycoside hydrolase, family 5	PFAM	PF00150	Cellulase	coord: 3..154 e-value: 1.2E-12 score: 47.9
IPR000772	Ricin B, lectin domain	PROSITE	PS50231	RICIN_B_LECTIN	coord: 204..337 score: 8.607
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 2..192
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 202..323

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G21442	Cucsat.G21442	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G21442.T2.E1	Cucsat.G21442.T2.E1	exon
Cucsat.G21442.T2.E2	Cucsat.G21442.T2.E2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G21442.T2.C1	Cucsat.G21442.T2.C1	CDS
Cucsat.G21442.T2.C2	Cucsat.G21442.T2.C2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G21442.T2	Cucsat.G21442.T2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0016985	mannan endo-1,4-beta-mannosidase activity