Cucsat.G21442 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G21442
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Mannan endo-1,4-beta-mannosidase
Location	ctg910: 3382515 .. 3384232 (+)
RNA-Seq Expression	Cucsat.G21442
Synteny	Cucsat.G21442

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGAATTACTCCATACTTTCTCATGAATATATGCCTCAACACTTTTTCTTTTTTCCTTTTTAAAACACATTCTAATGAACACTTGTTGGAATGCATGTTCATTCAAAAAGGTAAACCAAGCCTAAAGATTATTATTTATGATATGATCCCTCAATGCAGAAAAAGCCTGTTGAGTTTTTAAATTCCACAACACAAAGTTGGCCAACAACCTCCCATATTAACTTCTTTCTTGCCTTTGTTGATACCCACAAATATTTGCTTCAAGCACTATAAAAAATGGCTTTGGAGTACACCAAATTTCTCCTTTGTAGAAAAACCCAATGGAAATCACTACTCAATTCGGCTTTGTGGTTTTAGCTTTCATCTCTTTCTTTTCATCTCTTTCTTATTCTTTGCCTCTTTCGACAAACGGGAGATGGATTGTCGACTCCGCCACCGGGCGTCGAGTGAAGCTTGTGTGCGTGAATTGGCCTTCTCACACGCAAAGCATGTTAATCGAAGGCCTTGATCGCAGACCATTGAAAGACCTAGCAAATGAGGTAGTGCGATTGAGGTTTAATTGCGTGAGGCTCACATATGCAACTCACATGTTTACTCGATATGCTAATAGGACAGTTGAAGAGAATTTTGACCTTCTTGATTTGAGAGCTGCTAAGGTGGGGTTGGCTTTGCATAATCCGTTTGTATTGAATATGACCATTTTTGAAGCTTATGAAGCTGTGGTTGATGTGCTCGGGACCAGTGGTTTGATGGTTATAGCTGACAATCATATTAGCCAACCAAGATGGTGTTGCTCTCTTGAGGATGGAAATGGTTTCTTTGGAGATCGATATTTCGACACGGAAGAATGGTTGGAAGGTCTTCGTTTGGTTGCCAGACGATTCTACAACAAATCAGCAGTACGTAAATTCAATCTTCATACTTTTGAAATGATTTTTGTGAAATATGAAACAAATACATAACATAGACACATACACATTTTTATAAAAGTAAGTTACAAGTATAGTTCATTTGTTAAATTAAAGATCAATCCAAATATTTATTAAAATAAGTTAGGGAGGACTCGTGGGTACTCAGTTTGAGTTGTATTAAAGCACTTTTTAGACCCAACTCTATTCAACCATAAAATATATTTACGCTGCTTTGGCTCAGGTTCCTCGAGTCACTCAAGTCATCTATTCATCAAATAATTGGGTTCTTAATATATATTTAGAAAATGAAAATAGTACTAGAAAGTAATTAATTAGTAAGTAAAATTATGTCTTTCTTTGATAAATTTATGGGACTTTCACCTTCACGTCCAAGCAGGTGGTAGCCATGAGCCTACGTAATGAACTCCGAGGAGCAAGCTCAAAATCGAAAGATTGGAACAAGTACATAACACAAGGAGCAACAACAATCCACAACATAAACCCAAAGATCTTGGTAATCATTTCCGGTCTAAACTTCGACAACGATCTAAGATGCCAAAGGCAATACCCCTTGCAACTAAACAACCTACACAACAAGCTAGTATTTGAAGTACACTTATATTCCTTTAGTGGAGAATCCCAATCAAAGTTCATCCATAACCCACTCAACAAAATTTGCTCAAAGGTCATCAATGGGTTTGTGGAGAGAGCTGAGTTTGTGATGGAAGGAGCTGAGGCAGTTCCTTTGTTTGTGAGTGAATTTGGGTTGGACCAAAGGGGAGTTAATGAGGCTGATGATAGGTTTTTGAGTTGCTTTAGTGCTCATCTTGTTGAGAAAGATTTGGATTGGGCATTATGGGGTTGGCAAGGGAGCTATTATTATAGACAGGGTAAAGTAGGACCTGAAGAAGTGTTTGGAGTTTTGAATTACAATTGGAGTGATGTTAGAAACCCTCATTTTTCTCAGATGTTTCAACTCTTGCAAACCATGTTACAAGGTATGATTTCAAGTTCTATTTGATGACGCTTTTGTTTCGGTTTTTTTATTTTATAAAACGACTAAGTTCATTTTTCACTATACATAAATGATAAACTACAAAGGACAAAATGAAATTTTAAAAATGAACTTTTGAATAGAACATACGGTTAATGATAGTCTATTTTCTCTTGTTACTTTGTCTATCGAAAATACTCTTATACTTTCAAAAGTTTTTTAAAAAATTTAAACTCCTAAACAAGAGTTCAAAAATACTTTTGTCATTAGCTTTTCATAGAAACGGTTAGTATTTTATTTCAGAAATACTCATAAATTGTCAAAATTAATATCAACAAAGCTCCTTTAAGCTATAATAAAAAGAAAATAAAAAGTTGTTAAAAAAATACTCTTACATCCGCATATGAACAAAATCCATTAACATTTGTGAATCATATTAATATTAAATTTTTAATGATTTTACAACTCAACTTTTTTATGATTCGATAATAGCAATTGTGGTAGTACTTTTAGAGCTAATAATTAAGTGTATAGGAACACTTCAAAAGAATTACGAATATAATAAAATCCATCAATGATAGATATTATCGCTGATAGACTCCTAATAATAAAGTCTATTACTTATAGGCTTCGAGAGTTGGACTTAAATTTTACTATATCTATACATTTCTTTTATATTATGATATATTTGAAAATATTTTGGATATAATCTCTATATTTGTAACTAGCTTAATTTGATTTATAGATCCAAATTCCAATTCCTCAAACACTTACGTGATGTATCATCCACAAAGTGGGCAATGTGTCCTAGTGCAAGACATGAAACATATGCAAATTTATCTCAACGATTGTTCAAATGCAAGCCATTGGAGTTATGAAGGAGATGGAACTCCAATAATGTTGGCATCCACTAATTTTTGTCTAAAAGCCAGTGGAGATGGGCTTCCACCATCACTCTCAAGGGATTGTTTTGGTGAACAAAGTGTTTGGACAGCCATTTCAGATTCTAAGCTTCATTTGGCCACACTAACAAAGCAAGGCAATAATGGTATGTGTTTAGAGAAAGAGAGCTCAAATTCTAGTAGGATTTTGATGAGGAGTTGCGTTTGTGTTGGTAATGATTCAAATTGTTTACAAGATACTCAAGCTCAATGGTTTCAACTTGTTGTTACAAATACTTTGTAAATTGTTCACATGGGGAGATGAAATGGTGAAATAAGAAAGAGGGATTATTTAGTTTCAATTATTTGTTTCATGAGGACCTTTTGCCTTTTCTCTTCAAGTATTTTAACGTATACATATCTTAGGATGATTTTGAGTTTGATTTTTAAGTAAATTTTGTCGTCTTTGTTCGAGTGTATCCAAGTATTATTC

Coding sequence (CDS)

ATGGAAATCACTACTCAATTCGGCTTTGTGGTTTTAGCTTTCATCTCTTTCTTTTCATCTCTTTCTTATTCTTTGCCTCTTTCGACAAACGGGAGATGGATTGTCGACTCCGCCACCGGGCGTCGAGTGAAGCTTGTGTGCGTGAATTGGCCTTCTCACACGCAAAGCATGTTAATCGAAGGCCTTGATCGCAGACCATTGAAAGACCTAGCAAATGAGGTAGTGCGATTGAGGTTTAATTGCGTGAGGCTCACATATGCAACTCACATGTTTACTCGATATGCTAATAGGACAGTTGAAGAGAATTTTGACCTTCTTGATTTGAGAGCTGCTAAGGTGGGGTTGGCTTTGCATAATCCGTTTGTATTGAATATGACCATTTTTGAAGCTTATGAAGCTGTGGTTGATGTGCTCGGGACCAGTGGTTTGATGGTTATAGCTGACAATCATATTAGCCAACCAAGATGGTGTTGCTCTCTTGAGGATGGAAATGGTTTCTTTGGAGATCGATATTTCGACACGGAAGAATGGTTGGAAGGTCTTCGTTTGGTTGCCAGACGATTCTACAACAAATCAGCAGTGGTAGCCATGAGCCTACGTAATGAACTCCGAGGAGCAAGCTCAAAATCGAAAGATTGGAACAAGTACATAACACAAGGAGCAACAACAATCCACAACATAAACCCAAAGATCTTGGTAATCATTTCCGGTCTAAACTTCGACAACGATCTAAGATGCCAAAGGCAATACCCCTTGCAACTAAACAACCTACACAACAAGCTAGTATTTGAAGTACACTTATATTCCTTTAGTGGAGAATCCCAATCAAAGTTCATCCATAACCCACTCAACAAAATTTGCTCAAAGGTCATCAATGGGTTTGTGGAGAGAGCTGAGTTTGTGATGGAAGGAGCTGAGGCAGTTCCTTTGTTTGTGAGTGAATTTGGGTTGGACCAAAGGGGAGTTAATGAGGCTGATGATAGGTTTTTGAGTTGCTTTAGTGCTCATCTTGTTGAGAAAGATTTGGATTGGGCATTATGGGGTTGGCAAGGGAGCTATTATTATAGACAGGGTAAAGTAGGACCTGAAGAAGTGTTTGGAGTTTTGAATTACAATTGGAGTGATGTTAGAAACCCTCATTTTTCTCAGATGTTTCAACTCTTGCAAACCATGTTACAAGATCCAAATTCCAATTCCTCAAACACTTACGTGATGTATCATCCACAAAGTGGGCAATGTGTCCTAGTGCAAGACATGAAACATATGCAAATTTATCTCAACGATTGTTCAAATGCAAGCCATTGGAGTTATGAAGGAGATGGAACTCCAATAATGTTGGCATCCACTAATTTTTGTCTAAAAGCCAGTGGAGATGGGCTTCCACCATCACTCTCAAGGGATTGTTTTGGTGAACAAAGTGTTTGGACAGCCATTTCAGATTCTAAGCTTCATTTGGCCACACTAACAAAGCAAGGCAATAATGGTATGTGTTTAGAGAAAGAGAGCTCAAATTCTAGTAGGATTTTGATGAGGAGTTGCGTTTGTGTTGGTAATGATTCAAATTGTTTACAAGATACTCAAGCTCAATGGTTTCAACTTGTTGTTACAAATACTTTGTAA

Protein sequence

MEITTQFGFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDRRPLKDLANEVVRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRAAKVGLALHNPFVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDTEEWLEGLRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAEAVPLFVSEFGLDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPEEVFGVLNYNWSDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVLVQDMKHMQIYLNDCSNASHWSYEGDGTPIMLASTNFCLKASGDGLPPSLSRDCFGEQSVWTAISDSKLHLATLTKQGNNGMCLEKESSNSSRILMRSCVCVGNDSNCLQDTQAQWFQLVVTNTL

Homology

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BLAST of Cucsat.G21442 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 2.7e-97
Identity = 184/379 (48.55%), Postives = 238/379 (62.80%), Query Frame = 0


Query: 22  SYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDRRPLKDLANEVVRLRFNC 81
           S+SLPL T GRWIVD ATG RVKL CVNW  H +  L EGL+R P+  +A+ +  L FNC
Sbjct: 26  SHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVATVAHTISSLGFNC 85

Query: 82  VRLTYATHMFTR--YANRTVEENFDLLDLRAAKVGLALHNPFVLNMTIFEAYEAVVDVLG 141
           VRLTY+ HM TR  Y N TV + F  L+L  A  G+  +NP +L++    AY  VV  L 
Sbjct: 86  VRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLGHVAAYHHVVAALS 145

Query: 142 TSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDTEEWLEGLRLVARRFYNKSAVVAMSL 201
            +G+MVI DNH+S+P+WCC+++DGNGFFGDRYF+   W+EGL L+A  F N   VVAMSL
Sbjct: 146 EAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMATYFNNTPNVVAMSL 205

Query: 202 RNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQRQYPLQLNNLHN 261
           RNELRG  S    W++++  GA T+H  NPK+LVI+SGL FD DL      P+ L     
Sbjct: 206 RNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSFLPVLPVTL-PFKE 265

Query: 262 KLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAE--AVPLFVSEFGL 321
           K+V+E H YSF    ++       N +C      F     FV   A   A PLF+SEFG+
Sbjct: 266 KIVYEGHWYSFGVPWRTGL----PNDVCKNETGRFKSNVGFVTSSANATAAPLFMSEFGI 325

Query: 322 DQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGP---EEVFGVLNYNWS 381
           DQR VN+ D+R+L+C  A+L E+DLDWALW   GSYYYR  K      EE +G  N++WS
Sbjct: 326 DQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQPVKDFEETYGFFNHDWS 385

Query: 382 DVRNPHFSQMFQLLQTMLQ 394
            +RNP F    + +Q  +Q
Sbjct: 386 RIRNPDFISRLKEIQQPIQ 399

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BLAST of Cucsat.G21442 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 74.7 bits (182), Expect = 2.6e-12
Identity = 92/376 (24.47%), Postives = 150/376 (39.89%), Query Frame = 0


Query: 20  SLSYSLPLSTNGRWIVDSATGRRVKLVCVN-WPSHTQSMLIEGLDRRPLKDLANEVVRLR 79
           + SYS+    N R IVD  +G+ V+L  VN +   T + ++ GL  R  KD+  ++  L 
Sbjct: 23  AFSYSI---NNSRQIVDD-SGKVVQLKGVNVFGFETGNHVMHGLWARNWKDMIVQMQGLG 82

Query: 80  FNCVRLTYATHMFTRYANRTVEENFDLLDLRAAKVGLALHNPFVLNMTIFEAYEAVVDVL 139
           FN VRL +         + T+  + D              N  +  +T  +  + V+   
Sbjct: 83  FNAVRLPFCP---ATLRSDTMPASIDY-----------SRNADLQGLTSLQILDKVIAEF 142

Query: 140 GTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDTEEWLEGLRLVARRFYNKSAVVAMS 199
              G+ V+ D+H      C  + +    +    +   +WL  LR VA R+ N   V+ + 
Sbjct: 143 NARGMYVLLDHHTPD---CAGISE---LWYTGSYTEAQWLADLRFVANRYKNVPYVLGLD 202

Query: 200 LRNELRGAS-----SKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDL--------- 259
           L+NE  GA+     + + DWNK   +G+  +  + PK L+ + G+  DN +         
Sbjct: 203 LKNEPHGAATWGTGNAATDWNKAAERGSAAVLAVAPKWLIAVEGIT-DNPVCSTNGGIFW 262

Query: 260 --RCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVM 319
               Q      LN   N+L+   H+Y      QS F  N  N      +    ER     
Sbjct: 263 GGNLQPLACTPLNIPANRLLLAPHVYGPDVFVQSYF--NDSN--FPNNMPAIWERHFGQF 322

Query: 320 EGAEAVPLFVSEFGLDQRGVNEADDRFLSCFSAHLVEKDLDWAL-WGWQGSYYYRQGKVG 378
            G  A  L + EFG      +  D  +      +L  K ++    W W  +     G  G
Sbjct: 323 AGTHA--LLLGEFGGKYGEGDARDKTWQDALVKYLRSKGINQGFYWSWNPN----SGDTG 358

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BLAST of Cucsat.G21442 vs. NCBI nr
Match: XP_004143723.1 (glycosyl hydrolase 5 family protein [Cucumis sativus] >KAE8647936.1 hypothetical protein Csa_000506 [Cucumis sativus])

HSP 1 Score: 808 bits (2087), Expect = 4.73e-293
Identity = 394/399 (98.75%), Postives = 395/399 (99.00%), Query Frame = 0


Query: 1   MEITTQFGFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60
           MEITTQFGFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE
Sbjct: 1   MEITTQFGFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLANEVVRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRAAKVGLALHNP 120
           GLDRRPLKDLANEVVRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRAAKVGLA HNP
Sbjct: 61  GLDRRPLKDLANEVVRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRAAKVGLAFHNP 120

Query: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDTEEWLEG 180
           FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDTEEWLEG
Sbjct: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDTEEWLEG 180

Query: 181 LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNF 240
           LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNF
Sbjct: 181 LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNF 240

Query: 241 DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEF 300
           DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEF
Sbjct: 241 DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEF 300

Query: 301 VMEGAEAVPLFVSEFGLDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV 360
           VMEGAEAVPLFVSEFGLDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV
Sbjct: 301 VMEGAEAVPLFVSEFGLDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV 360

Query: 361 GPEEVFGVLNYNWSDVRNPHFSQMFQLLQTMLQGMISSS 399
           GPEEVFGVLNYNWSDVRNPHFSQMFQLLQTMLQ   S+S
Sbjct: 361 GPEEVFGVLNYNWSDVRNPHFSQMFQLLQTMLQDPNSNS 399

[Close]

BLAST of Cucsat.G21442 vs. NCBI nr
Match: KAA0055112.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 785 bits (2026), Expect = 9.21e-284
Identity = 382/399 (95.74%), Postives = 389/399 (97.49%), Query Frame = 0


Query: 1   MEITTQFGFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60
           M ITTQF FVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE
Sbjct: 1   MGITTQFSFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLANEVVRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRAAKVGLALHNP 120
           GLDRRPLKDLANEV+RLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRA+KVGLALHNP
Sbjct: 61  GLDRRPLKDLANEVMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLALHNP 120

Query: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDTEEWLEG 180
           FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFD+EEWLEG
Sbjct: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDSEEWLEG 180

Query: 181 LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNF 240
           LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKY+TQGATTIHNINP ILVIISGLNF
Sbjct: 181 LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNF 240

Query: 241 DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEF 300
           DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSK+INGFV+RAEF
Sbjct: 241 DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEF 300

Query: 301 VMEGAEAVPLFVSEFGLDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV 360
           VMEGAEAVPLFVSEFGLDQ GVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV
Sbjct: 301 VMEGAEAVPLFVSEFGLDQTGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV 360

Query: 361 GPEEVFGVLNYNWSDVRNPHFSQMFQLLQTMLQGMISSS 399
             EEVFGVLNYNWSDVRNP FSQMFQLLQTMLQ   S+S
Sbjct: 361 ELEEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNS 399

[Close]

BLAST of Cucsat.G21442 vs. NCBI nr
Match: XP_008467306.1 (PREDICTED: major extracellular endoglucanase-like [Cucumis melo])

HSP 1 Score: 780 bits (2013), Expect = 8.79e-282
Identity = 379/399 (94.99%), Postives = 387/399 (96.99%), Query Frame = 0


Query: 1   MEITTQFGFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60
           M ITTQF FVVLAFI FFSSLSYSLPLSTNGRWIVDSATG RVKLVCVNWPSHTQSMLIE
Sbjct: 1   MGITTQFSFVVLAFICFFSSLSYSLPLSTNGRWIVDSATGHRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLANEVVRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRAAKVGLALHNP 120
           GLDRRPLKDLANEV+RL+FNCVRLTYATHMFTRYANRTVEENFDLLDLRA+KVGLALHNP
Sbjct: 61  GLDRRPLKDLANEVMRLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLALHNP 120

Query: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDTEEWLEG 180
           FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFD+EEWLEG
Sbjct: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDSEEWLEG 180

Query: 181 LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNF 240
           LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKY+TQGATTIHNINP ILVIISGLNF
Sbjct: 181 LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNF 240

Query: 241 DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEF 300
           DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSK+INGFV+RAEF
Sbjct: 241 DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEF 300

Query: 301 VMEGAEAVPLFVSEFGLDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV 360
           VMEGAEAVPLFVSEFGLDQ GVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV
Sbjct: 301 VMEGAEAVPLFVSEFGLDQTGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV 360

Query: 361 GPEEVFGVLNYNWSDVRNPHFSQMFQLLQTMLQGMISSS 399
             EEVFGVLNYNWSDVRNP FSQMFQLLQTMLQ   S+S
Sbjct: 361 ELEEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNS 399

[Close]

BLAST of Cucsat.G21442 vs. NCBI nr
Match: XP_038875504.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 732 bits (1890), Expect = 4.34e-263
Identity = 352/396 (88.89%), Postives = 372/396 (93.94%), Query Frame = 0


Query: 4   TTQFGFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLD 63
           TTQ  FV LAFISFFS LSYS PLSTNGRWIVDSA GRR+KL+CVNWPSHTQSMLIEGLD
Sbjct: 4   TTQINFVFLAFISFFS-LSYSFPLSTNGRWIVDSA-GRRMKLMCVNWPSHTQSMLIEGLD 63

Query: 64  RRPLKDLANEVVRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRAAKVGLALHNPFVL 123
           RRPLKDLA+EVVRLRFNCVRLTYATHMFTRYANRTV+ENFDLLDL+A+K+GLALHNPF+L
Sbjct: 64  RRPLKDLADEVVRLRFNCVRLTYATHMFTRYANRTVQENFDLLDLKASKMGLALHNPFIL 123

Query: 124 NMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDTEEWLEGLRL 183
           NMT+F AYEA VD+LGTSGLMVIADNHISQP+WCCSL+DGNGFFGDRYFD EEWLEGLRL
Sbjct: 124 NMTVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLRL 183

Query: 184 VARRFYNKSAVVAMSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDND 243
           VARRFYNKS VVAMSLRNELRG  SKSKDWNKY+TQGATTIH INP IL+IISGLNFDND
Sbjct: 184 VARRFYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNFDND 243

Query: 244 LRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVME 303
           LRCQRQ PL LNNLHNKLVFEVHLYSFSGESQSKFI+NPLNKICSK+INGFVERAEFVME
Sbjct: 244 LRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEFVME 303

Query: 304 GAEAVPLFVSEFGLDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPE 363
           GAEAVPLFVSEFG DQRGVNEADDRFLSCF AHL +KDLDWALW WQGSYYYRQGK+ PE
Sbjct: 304 GAEAVPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKIEPE 363

Query: 364 EVFGVLNYNWSDVRNPHFSQMFQLLQTMLQGMISSS 399
           EVFGVLNYNW+DVRNPHFSQMFQLLQTMLQ   S+S
Sbjct: 364 EVFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNS 397

[Close]

BLAST of Cucsat.G21442 vs. NCBI nr
Match: XP_022995241.1 (uncharacterized protein LOC111490847 [Cucurbita maxima])

HSP 1 Score: 686 bits (1769), Expect = 1.12e-244
Identity = 328/385 (85.19%), Postives = 350/385 (90.91%), Query Frame = 0


Query: 9   FVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDRRPLK 68
           F++LAFIS FSSLS SLPLST GRWIVDSATGRRVKLVCVNWPSHTQSMLIEGL  RPLK
Sbjct: 8   FMLLAFISLFSSLSNSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLAHRPLK 67

Query: 69  DLANEVVRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRAAKVGLALHNPFVLNMTIF 128
           DLA+E+V L+FNCVRLTYATHMFTRYANRTVEENFDLLDLRA+K GLA HNPF+LNMTIF
Sbjct: 68  DLADELVNLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKAGLASHNPFILNMTIF 127

Query: 129 EAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDTEEWLEGLRLVARRF 188
           +AYEAVVDVLGTSGLMVIADNHISQPRWCCSL DGNGFFGDRYFD +EWLEGLRLVARRF
Sbjct: 128 DAYEAVVDVLGTSGLMVIADNHISQPRWCCSLNDGNGFFGDRYFDPQEWLEGLRLVARRF 187

Query: 189 YNKSAVVAMSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQR 248
            NK  VVAMSLRNELRGA S SKDWNKY+TQGATTIH+INP +LVI+SGLNFDNDLRCQR
Sbjct: 188 TNKLNVVAMSLRNELRGAKSSSKDWNKYMTQGATTIHDINPNLLVIVSGLNFDNDLRCQR 247

Query: 249 QYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAEAV 308
             PL LNNLHNKLVFEVHLYSFSG +++KFI NPLNKICS +INGFVERAEFVM+GAEAV
Sbjct: 248 HNPLPLNNLHNKLVFEVHLYSFSGATKTKFITNPLNKICSAIINGFVERAEFVMQGAEAV 307

Query: 309 PLFVSEFGLDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPEEVFGV 368
           PLFVSEFG DQRG N ADDRF SCF AHL   DLDWALW WQGSYYYRQG+   +EVFGV
Sbjct: 308 PLFVSEFGFDQRGTNVADDRFSSCFVAHLARTDLDWALWAWQGSYYYRQGQAQSDEVFGV 367

Query: 369 LNYNWSDVRNPHFSQMFQLLQTMLQ 393
           LNYNWSDVRNPHFS+ FQLLQTML+
Sbjct: 368 LNYNWSDVRNPHFSKTFQLLQTMLR 392

[Close]

BLAST of Cucsat.G21442 vs. ExPASy TrEMBL
Match: A0A5A7UH07 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00380 PE=3 SV=1)

HSP 1 Score: 785 bits (2026), Expect = 4.46e-284
Identity = 382/399 (95.74%), Postives = 389/399 (97.49%), Query Frame = 0


Query: 1   MEITTQFGFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60
           M ITTQF FVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE
Sbjct: 1   MGITTQFSFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLANEVVRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRAAKVGLALHNP 120
           GLDRRPLKDLANEV+RLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRA+KVGLALHNP
Sbjct: 61  GLDRRPLKDLANEVMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLALHNP 120

Query: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDTEEWLEG 180
           FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFD+EEWLEG
Sbjct: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDSEEWLEG 180

Query: 181 LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNF 240
           LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKY+TQGATTIHNINP ILVIISGLNF
Sbjct: 181 LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNF 240

Query: 241 DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEF 300
           DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSK+INGFV+RAEF
Sbjct: 241 DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEF 300

Query: 301 VMEGAEAVPLFVSEFGLDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV 360
           VMEGAEAVPLFVSEFGLDQ GVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV
Sbjct: 301 VMEGAEAVPLFVSEFGLDQTGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV 360

Query: 361 GPEEVFGVLNYNWSDVRNPHFSQMFQLLQTMLQGMISSS 399
             EEVFGVLNYNWSDVRNP FSQMFQLLQTMLQ   S+S
Sbjct: 361 ELEEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNS 399

[Close]

BLAST of Cucsat.G21442 vs. ExPASy TrEMBL
Match: A0A1S3CTF8 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103504686 PE=3 SV=1)

HSP 1 Score: 780 bits (2013), Expect = 4.26e-282
Identity = 379/399 (94.99%), Postives = 387/399 (96.99%), Query Frame = 0


Query: 1   MEITTQFGFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60
           M ITTQF FVVLAFI FFSSLSYSLPLSTNGRWIVDSATG RVKLVCVNWPSHTQSMLIE
Sbjct: 1   MGITTQFSFVVLAFICFFSSLSYSLPLSTNGRWIVDSATGHRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLANEVVRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRAAKVGLALHNP 120
           GLDRRPLKDLANEV+RL+FNCVRLTYATHMFTRYANRTVEENFDLLDLRA+KVGLALHNP
Sbjct: 61  GLDRRPLKDLANEVMRLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLALHNP 120

Query: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDTEEWLEG 180
           FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFD+EEWLEG
Sbjct: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDSEEWLEG 180

Query: 181 LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNF 240
           LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKY+TQGATTIHNINP ILVIISGLNF
Sbjct: 181 LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNF 240

Query: 241 DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEF 300
           DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSK+INGFV+RAEF
Sbjct: 241 DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEF 300

Query: 301 VMEGAEAVPLFVSEFGLDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV 360
           VMEGAEAVPLFVSEFGLDQ GVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV
Sbjct: 301 VMEGAEAVPLFVSEFGLDQTGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV 360

Query: 361 GPEEVFGVLNYNWSDVRNPHFSQMFQLLQTMLQGMISSS 399
             EEVFGVLNYNWSDVRNP FSQMFQLLQTMLQ   S+S
Sbjct: 361 ELEEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNS 399

[Close]

BLAST of Cucsat.G21442 vs. ExPASy TrEMBL
Match: A0A6J1K1F7 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111490847 PE=3 SV=1)

HSP 1 Score: 686 bits (1769), Expect = 5.43e-245
Identity = 328/385 (85.19%), Postives = 350/385 (90.91%), Query Frame = 0


Query: 9   FVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDRRPLK 68
           F++LAFIS FSSLS SLPLST GRWIVDSATGRRVKLVCVNWPSHTQSMLIEGL  RPLK
Sbjct: 8   FMLLAFISLFSSLSNSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLAHRPLK 67

Query: 69  DLANEVVRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRAAKVGLALHNPFVLNMTIF 128
           DLA+E+V L+FNCVRLTYATHMFTRYANRTVEENFDLLDLRA+K GLA HNPF+LNMTIF
Sbjct: 68  DLADELVNLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKAGLASHNPFILNMTIF 127

Query: 129 EAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDTEEWLEGLRLVARRF 188
           +AYEAVVDVLGTSGLMVIADNHISQPRWCCSL DGNGFFGDRYFD +EWLEGLRLVARRF
Sbjct: 128 DAYEAVVDVLGTSGLMVIADNHISQPRWCCSLNDGNGFFGDRYFDPQEWLEGLRLVARRF 187

Query: 189 YNKSAVVAMSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQR 248
            NK  VVAMSLRNELRGA S SKDWNKY+TQGATTIH+INP +LVI+SGLNFDNDLRCQR
Sbjct: 188 TNKLNVVAMSLRNELRGAKSSSKDWNKYMTQGATTIHDINPNLLVIVSGLNFDNDLRCQR 247

Query: 249 QYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAEAV 308
             PL LNNLHNKLVFEVHLYSFSG +++KFI NPLNKICS +INGFVERAEFVM+GAEAV
Sbjct: 248 HNPLPLNNLHNKLVFEVHLYSFSGATKTKFITNPLNKICSAIINGFVERAEFVMQGAEAV 307

Query: 309 PLFVSEFGLDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPEEVFGV 368
           PLFVSEFG DQRG N ADDRF SCF AHL   DLDWALW WQGSYYYRQG+   +EVFGV
Sbjct: 308 PLFVSEFGFDQRGTNVADDRFSSCFVAHLARTDLDWALWAWQGSYYYRQGQAQSDEVFGV 367

Query: 369 LNYNWSDVRNPHFSQMFQLLQTMLQ 393
           LNYNWSDVRNPHFS+ FQLLQTML+
Sbjct: 368 LNYNWSDVRNPHFSKTFQLLQTMLR 392

[Close]

BLAST of Cucsat.G21442 vs. ExPASy TrEMBL
Match: A0A6J1H201 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459707 PE=3 SV=1)

HSP 1 Score: 681 bits (1757), Expect = 3.63e-243
Identity = 327/384 (85.16%), Postives = 349/384 (90.89%), Query Frame = 0


Query: 10  VVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDRRPLKD 69
           ++LAFIS FSSLS SLPLST GRWIVDS TGRRVKLVCVNWPSHTQSMLIEGL  RPLKD
Sbjct: 9   MLLAFISLFSSLSNSLPLSTRGRWIVDSTTGRRVKLVCVNWPSHTQSMLIEGLANRPLKD 68

Query: 70  LANEVVRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRAAKVGLALHNPFVLNMTIFE 129
           LA+E+V L+FNCVRLTYATHMFTRYANRTVEENFDLLDLRA+KVGLA HNPFVLNMTIFE
Sbjct: 69  LADELVSLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLASHNPFVLNMTIFE 128

Query: 130 AYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDTEEWLEGLRLVARRFY 189
           AYE VVDVLGTSGLMVIADNHISQPRWCCSL DGNGFFGDRYFD +EWLEGLRLVARRF 
Sbjct: 129 AYETVVDVLGTSGLMVIADNHISQPRWCCSLNDGNGFFGDRYFDPQEWLEGLRLVARRFT 188

Query: 190 NKSAVVAMSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDLRCQRQ 249
           NKS VVAMSLRNELRGA S SKDWNKY+TQGATTIH+INP +LVI+SGLNFDNDLRCQR 
Sbjct: 189 NKSNVVAMSLRNELRGAKSSSKDWNKYMTQGATTIHDINPNLLVIVSGLNFDNDLRCQRH 248

Query: 250 YPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEGAEAVP 309
            PL LNNLHNKLVFEVHLYSFSG +++KFI NPLNKICS +INGFVERAEFVM+GAEAVP
Sbjct: 249 NPLPLNNLHNKLVFEVHLYSFSGATKTKFITNPLNKICSTIINGFVERAEFVMQGAEAVP 308

Query: 310 LFVSEFGLDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPEEVFGVL 369
           LFVSEFG DQRG N ADDRFLSCF AHL + DLDWALW WQGSYYYRQG+   +EVFG+L
Sbjct: 309 LFVSEFGFDQRGTNVADDRFLSCFVAHLAKTDLDWALWAWQGSYYYRQGQAQFDEVFGIL 368

Query: 370 NYNWSDVRNPHFSQMFQLLQTMLQ 393
           NYNWS VRNP FS+ FQLLQTML+
Sbjct: 369 NYNWSGVRNPRFSKTFQLLQTMLR 392

[Close]

BLAST of Cucsat.G21442 vs. ExPASy TrEMBL
Match: A0A6J1DXU9 (Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111025142 PE=3 SV=1)

HSP 1 Score: 669 bits (1726), Expect = 2.10e-238
Identity = 323/391 (82.61%), Postives = 345/391 (88.24%), Query Frame = 0


Query: 4   TTQFGFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLD 63
           +T+  FV LA IS  SSLSYSLPLST GRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLD
Sbjct: 3   STKETFVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLD 62

Query: 64  RRPLKDLANEVVRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRAAKVGLALHNPFVL 123
           RRPLKDLA+E VRLRFNCVRLTYATHMFTRYANRT+EENFDLLDL+A+K GLALHNPFVL
Sbjct: 63  RRPLKDLADEAVRLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKASKAGLALHNPFVL 122

Query: 124 NMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDTEEWLEGLRL 183
           NMTIFEAYEA VDVLG SGLMVIADNHISQPRWCCSL+DGNGFFGDRYFD EEWLEGLRL
Sbjct: 123 NMTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEGLRL 182

Query: 184 VARRFYNKSAVVAMSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDND 243
           VAR F+NKS+VV MSLRNELRGA S  KDWNK+IT+GA  IH INP  LVIISGLN+DND
Sbjct: 183 VARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNYDND 242

Query: 244 LRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVME 303
           LRCQ Q PL L NLHNKLV EVHLYSFSG+SQ+KF  NPLNKICSKV+NGFVERA FV+E
Sbjct: 243 LRCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGFVVE 302

Query: 304 GAEAVPLFVSEFGLDQR-GVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGP 363
           G  AVPL VSEFG+DQR   NEADDRF+SCF A+L   DLDWA+WGWQGSYYYRQG+  P
Sbjct: 303 GERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQARP 362

Query: 364 EEVFGVLNYNWSDVRNPHFSQMFQLLQTMLQ 393
            EVFGVLNY+WS VRNP FSQ FQLLQTMLQ
Sbjct: 363 REVFGVLNYDWSGVRNPRFSQRFQLLQTMLQ 393

The following BLAST results are available for this feature:

BLAST of Cucsat.G21442 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0HLA0	2.7e-97	48.55	Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
P19487	2.6e-12	24.47	Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra...	[more]

BLAST of Cucsat.G21442 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143723.1	4.73e-293	98.75	glycosyl hydrolase 5 family protein [Cucumis sativus] >KAE8647936.1 hypothetical...	[more]
KAA0055112.1	9.21e-284	95.74	major extracellular endoglucanase-like [Cucumis melo var. makuwa]	[more]
XP_008467306.1	8.79e-282	94.99	PREDICTED: major extracellular endoglucanase-like [Cucumis melo]	[more]
XP_038875504.1	4.34e-263	88.89	glycosyl hydrolase 5 family protein-like [Benincasa hispida]	[more]
XP_022995241.1	1.12e-244	85.19	uncharacterized protein LOC111490847 [Cucurbita maxima]	[more]

BLAST of Cucsat.G21442 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UH07	4.46e-284	95.74	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A1S3CTF8	4.26e-282	94.99	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103504686 PE=3 SV...	[more]
A0A6J1K1F7	5.43e-245	85.19	Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111490847 PE=...	[more]
A0A6J1H201	3.63e-243	85.16	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459707 P...	[more]
A0A6J1DXU9	2.10e-238	82.61	Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111025142 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 25..394 e-value: 4.6E-66 score: 225.4
None	No IPR available	PANTHER	PTHR31263	CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)	coord: 9..535
IPR001547	Glycoside hydrolase, family 5	PFAM	PF00150	Cellulase	coord: 66..350 e-value: 3.7E-24 score: 85.6
IPR000772	Ricin B, lectin domain	PROSITE	PS50231	RICIN_B_LECTIN	coord: 400..533 score: 8.607
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 398..519
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 26..388

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G21442.T1	Cucsat.G21442.T1	mRNA
Cucsat.G21442.T3	Cucsat.G21442.T3	mRNA
Cucsat.G21442.T2	Cucsat.G21442.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0016985	mannan endo-1,4-beta-mannosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds