Cucsat.G19868.T5 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G19868.T5
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionvesicle-associated protein 2-2 isoform X1
Locationctg4: 2336253 .. 2341845 (+)
RNA-Seq ExpressionCucsat.G19868.T5
SyntenyCucsat.G19868.T5
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAAAAGAAAAAGCCTCGAAATTGGAGCCAATTTTGTTAAATTATTTGAAAAGAAAAGAGAAAGGGAAGAAAGTTCAAACGGGAATTGGATTCTGCCGCACCGGCTCAAAGCTAAGATCACACCACTGCTGGTACAACTAACACTACCTCTCTCTCTCTCTCTACTTTGCTTTCACTCAGTTTAATCCTCCATTCCACCAACTAGATCATTCATTCTCACCTTCCTCTCTCTAGAATTTTCCAGGGTTTCTCTCTCTCTCTCCCTTCATATGGAATTGTAAATTCCACCAAAATCTTTGGACTCTCTTCCAATTTCTGCATTCCATTCTCTTTTATTTATTCCATTTCAGTTTATCTTTCTCTCTCTATCTCCGTTCCCGCGGAACTGGTGAGTCTTTTGGATTCTTTTTCCGCTTCTTGTTTTATATTCTGCGTGGAAGAATCTTATTGTTTGGTTATTTTTGTTGTTTGAAATTCATGGTATCTTGTTAAGAACGCAGTCTGTGTGTTTTTTCCTGAATTTCTGCTGGTTTCTTGAATATTGGAGTTTCTTTTTTGCCTCTCGATTTGTGTTTGATTTTGTTGAGTTTGGAAATGGGTTGGAGTTTGTAATCGGATTAGTGTTTGGAGTGACTCTGTTCGTTGACTAATGATTTATGGTTCGAGTAGTGTTTGGTTTGTAACTTTGAACGATCTGTTTTGGAATAAGCCCAGCGGTGCGTCTTTGATTTTGATCTTAGTGGGTTTGGTTTGACTTTCTCAACCCAGTTTTTTTTTTTCCTTGGAAAGTTGGGGAAATGGGATATTATTATTATTTTATTTTAGCCAGGAACAGGGTGGATTGGAAACACGTGGTTTTTAGCTTCTATGATCTGTAATTATATGATGAAATACTTGGGATTATTCTATGTTTCTTTTTTTCTTTCTTTTTATTTTTTATTTTTTGAATTTTCTTCTAAATCAAATTTAATTTGTTTTTCCCGATTATAAGAAAATAACGTTTCTGTGTGCAGAGTAATTGTTTCCCAGGCTTTGAATGCAGAAGTATTAATTTTGAATTGTGTATTGTGAGGAAGAGACATGAGTACGAAACTCTTGCATATCCAGCCTAAGGAATTACAATTCTTATGTAAGTGCATTACTAGTAATACTAGTCATTGCTTGCTTTTTGAAAAAGAATTGATGTAAAGTACCTTGCTACTTTCAGTTGAATTGAGGAAACAAAGTACGTGCACAGTTCAACTATCCAATAATACCCATCATCATGTTGCCTTTAAAGTATGAACAACCTTCTTTTCTTTTTGTTCATCTCTTGTTTCTTTGATAATTCATATGATTTTTCATGTCATGTGTGCAATGTTATTATTATGTTTGGCAGGTTAAAACTACGTCTCCTAAGAAATATTGTGTTCGACCAAATGTTGGGATAATTTTGCCAAAATCAACATGTGAATTTTCAGGTACATTCTTTATAATTTTCTTCTAATCTAACATATTTGGCCACCTGGGACATTTGATTCCCGGTCCCCCAGAAACAAAAAAGAGAGTGTTTCTAGATATTTTAACGAAGTTACTACCATGATGATTGACGTTTCAATGAAGTTGATTCTTCCATCTAGTACTTGACATGTACAGTATCTGATCGGCTCAATGTCTTTCTTTAGTTGAATCAAAATTATGAGGACTATCTGGAAAGTTTTAAAAGGGAAATTTGAGAACATATCACTTTAGTGTTTTGAGGAATAAAAACTAAGAATGTCTAATATCATGTCATGATTCAATCCTGCATTTAACAACTATTTCTCCCTTACAGTTTCTTTTAGGAAGACTGTGCAACTGTCTTACTGTAATACTTGCGTCCTTTTTTTTTCTTTGCTTTTGTTCTTGTTTTTCCTAAAAAGAAAACCTAAACCTGGAAGTTGGCTGTGTATTGTTGCTTTTCCTCCTCTTTTTAGAAGCTAATTTCTTCTATGATTCTGACGTAGTTATAATGCAAGCTCAACGGGCTGCCCCACCTGATATGTTATGTAAAGACAAGTTTTTAATTCAAAGCACAATCGTTCCTTCAGGGATAACAGAAGAGGACATCACTGCAAGTATGGTAAGTTTTTTGGATACATTCAGCTTTCATGCCTTGGGTTTTGAACTGTTTACATTTCTTTCCAGTTTTCAAAAGATGGAGGCAAATATATTGAAGAGGATAAGCTCAAAGTGGCGCTCATTAGTCCGCTGAATTCCCCAATACTCTCGCCTTCTGATGGAGCATTGGACGCTAGAATGGATGACGTTATGAAATTGAATGAAGATAGTGGACCATTAAGTGATGGGATGGACACCAATGAAACCCCAAAGCTGAAAGATCAAAATGGTGCATCAGATGAGGTACTTCCCTCCGTGGTTCCAATCCTAAGATATCAAAATGGACTATCAAATGAGGGCATGACCCATGAGGCTTCAAGATTCAAAGATGATTTTGTTGCATTGAAGGGGGAGCGGACCATTGAAGCTTTAAAGCCATCTGATCAAAATGGAGCATTCAGGGAGGAGGTTCTGCCTCATGAGTTTTCAAAACTGAAGGATCCAAATGAAACATCAAAGGAAGAGCTACCTCGTGAAGCATTAAAACCGAGAGATCAAAATCGAGCATTAAAGGAAGAGGAGTTGAGTGTTGAATCACCAACAAAAAAAGTTCAAAATGGTGCACTGAAGTGGGGAGCCTACGGAAGTTCACATCTGGAGAATCCAGTACCAGACACAAAAGTAAGAAAAAACTTTCCTTATCAAATTTGATGATATACTGACTAATTTTTGTTGTTCTAAGAAATTTTTGGAGCCAGAGGTCTCTTTATAGTAACCTCTTCCACATTTCCTAGGGAGGGTGCCTTATCTTTTCCTTGTTTTTCCCAGTAGAAAGTGTTTCTTTTCTAGAGAGGAAAAAAAAAAATTGTAGTAGGAAACTGTAGACCGTGTCATGATTTAGGCATTGTTCAATTTGAGATTTTCAGTTTCCTGTGCATTGGCTTCTAGAACATTGAAGTTTTTCTCTTGTGCATCAGTTTCCTGAGGATGCGGATGAATCCCTAGAGCTAAACAATAGCATGTCAAGATTACAAAAGACTGCTGAGATAAAGCAAGGCACAGAATTGGAACCTTTGAAACTGAAAAAAGCGGAACAGCTACAGTTAGTTAAGGACATTGATGAGATGAAATCGAAGCTACATGAAATTGAATTAAAGCTTGGAGAGGTGATTTTTTATTGCCTTTTGTGCTTTTCTCGTTCTTGTGCTGCCTTTAAGCTATTATTGAAAATCTGATCATTGAGATTGTTGATCCTCACCTAATTCAATTAAGAAATAGGACTGTGATTGATTTTTAATTTTGTTGATGCGCATGCGTATTCAATTTATCTATGAACTTACCATAACAGTTGATTTGTAGAAAGAACCAGCGACCTATGTCAAACAACTTTTAGATAAAAAACTGAAATTTATTCAGAAAAAGAGAGCTTGTTAAATCATCATTTGACCCTAAAGCTTAAACTAGTGGGTTATGGTAAATTTTATTATACAAACACTTAAACACTCTTACCCACTTTTGGGCTTGAAATTTTGTAGATGACCGAATGAATAGAAATTAATATTATGTGGAGAGAAAATGACATTACACCTATAGAAGGGAGGGGAGGAGAGGGGGGGGAGGGGAGGGGGAGGGGAGGGCGACGAGTTCTCCCTAAAAATCTTTGTAGAATATTAAGAAAATCACTCCCAAAAGAATTAGTTCTGAAACCATTAGAAAGAAATCTTCATAAAGAAGCCAAAAAATCAAGCCTTGTCCCGAAAATGATCAAGAATCTTATATGCCGTTTGAAAGTTTGCACTCTGTTTGTTAAAACAATTTCCTGATGAGATTCATTTCACCCTGTTGCAAATTGCACTCCCTGAAATTTGTTAAACTTGGTTCATTTGCTAATTCATTTGGATGTTCAGTAATGCAACTTTTGTGGTTGATTTTGATTTTTCACTGCATCTAGATAAGCTTGGTTTTGTGATACAGGTTGATTCTTAAAATTTGTTTAAACTTGGTCTCAGTTGCCGGTTATCATCTGTTTTTTTTCCTTTTCCCTTCGAGGTTTGCAATGTTTTTCCTTAATTAGATGAAGAATTAGGCGAGGGCTTCCTTTAATCATAATCATCTCAGTACCCAAGAAATGAAATGTTGATGGAAAGGAAAGAAACTACTTCACTAATATTCATTTTTCATTTGCCGTATTATTGTACTTTTTATTGACCAACACAATTGTTAATATGTTTTTTTGCCACATCATTTAATAGGCTCAAGGTACCATTTCAATGCTGTCTGAAGCGAGGAGATTAAGTGCTCAAGAAGTGAAAATCTTGAAGGAGAAATTGGTAACTTCCTGGACCTTATTTTTCTGTTAGTGGATGTTCTTCTTCCATGTTAGAGTCTTATGATGTCCATTTGATATTCAAGCCTTGCCACCCCAAGGGACCCTAATATTAGAGAGATTAGGTACCTTTTTCCCCAGCCGTAAGGATTGGAGATGTATTCAAGGTACTAGGTTGAAACTTCTCGCTTGAAAATTTGAAATCGTTACTGTCTCACTTTGGAGTGGTTGAAGTCCTCTCAGGGTGTACGGGCCTCTCAGTTTTAGTTGGTGGAATGGGATATTATGGCCCACGGGGCTCTAACTATAACAGATTAACTCAGCGCCCCAGCACCACCTCCTTACAATTTAGTTTGAGGTGTACTAATACTAATGCAAAGTAGAAGGAATAGAGTGCCTAAATGTCTGTTGTCCCATCAAGAAAAAATAGTGCAGGGGTAGCTTTAGTGGAGCCATCTCAAAAGTAATATTGTTATTTAGTTCACTTGTTTTGATCTTTTTGGACACTCTGCTCTCAGAAATTCATTCTTAACTGTACTTGTGCTTCCGGATTTTGTATACTATTGGATTTCAGCTGGTTCCTCAATAGCTGCATTCATGGTTTCTCTCACTTCCATTTCTTCTATCATTACTTCTCGTGACTCAGTGTTTTATCTTACTATTTTCTCAGCTGGAACTGAGTAGGAGGGGCAGGGCGAGCAATCAAGTCGGTTTTCCTCCTTTGTTTATCTGTATGGTGGCGTTAATTTGCATTGTGTTCGGATTCGTTTTGCACCATTGAAGTGTAAGGTGGGGAGGAGATGAGATGAGGATTGCAGAAAATATGTAAAGAAAAATATTTTACTTATGTAGATAGATCAGTAGCTAGTGCGGTGTGTTGTCAATAAGATGCACATTTTGATTTTGGGCACTGGAAGTTTGCTGCTCTTAGTATGCAATTCAGATTTTGGGTATAAATCTAACACGATGCTCATCTGCTTTTACCAATGTTTGAAGATGAGTTGTGATGGTTGACCAAATGTTGGGTTTCATATTGGTCTTCTTTTCTCTCTTTTCCCTTTTTCTTTTTGTGATTTGTTTCTGAGTTAGTCTATGTGCTGATTCTAACCGAATTTGAACTTTTTTTTTTTTGTATTGGAAAGTCATACTTGTTCAGTTGTGTTCTAATATGAGCATTTTTGGTCTTC

Coding sequence (CDS)

ATGGATGACGTTATGAAATTGAATGAAGATAGTGGACCATTAAGTGATGGGATGGACACCAATGAAACCCCAAAGCTGAAAGATCAAAATGGTGCATCAGATGAGGTACTTCCCTCCGTGGTTCCAATCCTAAGATATCAAAATGGACTATCAAATGAGGGCATGACCCATGAGGCTTCAAGATTCAAAGATGATTTTGTTGCATTGAAGGGGGAGCGGACCATTGAAGCTTTAAAGCCATCTGATCAAAATGGAGCATTCAGGGAGGAGGTTCTGCCTCATGAGTTTTCAAAACTGAAGGATCCAAATGAAACATCAAAGGAAGAGCTACCTCGTGAAGCATTAAAACCGAGAGATCAAAATCGAGCATTAAAGGAAGAGGAGTTGAGTGTTGAATCACCAACAAAAAAAGTTCAAAATGGTGCACTGAAGTGGGGAGCCTACGGAAGTTCACATCTGGAGAATCCAGTACCAGACACAAAATTTCCTGAGGATGCGGATGAATCCCTAGAGCTAAACAATAGCATGTCAAGATTACAAAAGACTGCTGAGATAAAGCAAGGCACAGAATTGGAACCTTTGAAACTGAAAAAAGCGGAACAGCTACAGTTAGTTAAGGACATTGATGAGATGAAATCGAAGCTACATGAAATTGAATTAAAGCTTGGAGAGGCTCAAGGTACCATTTCAATGCTGTCTGAAGCGAGGAGATTAAGTGCTCAAGAAGTGAAAATCTTGAAGGAGAAATTGCTGGAACTGAGTAGGAGGGGCAGGGCGAGCAATCAAGTCGGTTTTCCTCCTTTGTTTATCTGTATGGTGGCGTTAATTTGCATTGTGTTCGGATTCGTTTTGCACCATTGA

Protein sequence

MDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVLPSVVPILRYQNGLSNEGMTHEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFSKLKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPVPDTKFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRASNQVGFPPLFICMVALICIVFGFVLHH
Homology
BLAST of Cucsat.G19868.T5 vs. ExPASy Swiss-Prot
Match: B9DHD7 (Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 4.8e-08
Identity = 42/113 (37.17%), Postives = 68/113 (60.18%), Query Frame = 0

Query: 174 NSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLS 233
           +S  ++ K  ++ Q  + +   ++  ++L+LVKDI+EMK K+  +E KL +A  TIS L 
Sbjct: 273 DSEPKISKERDVVQLQKTDGQNVRGLDELKLVKDIEEMKLKVDALESKLKQADSTISKLM 332

Query: 234 EARRLSAQEVKILKEKLLELSRRGRASNQV--GFPPLFICMVALICIVFGFVL 285
           E R +S+Q  + L+ +L EL R  +   +V  GFP L++C+VA I  V G  L
Sbjct: 333 EERSISSQHRQSLQHELAEL-RTKKIVKEVHNGFPLLYVCVVAFIAYVIGHFL 384

BLAST of Cucsat.G19868.T5 vs. NCBI nr
Match: XP_011648789.1 (vesicle-associated protein 2-2 isoform X1 [Cucumis sativus] >XP_011648790.1 vesicle-associated protein 2-2 isoform X1 [Cucumis sativus] >XP_031737157.1 vesicle-associated protein 2-2 isoform X1 [Cucumis sativus])

HSP 1 Score: 546 bits (1408), Expect = 2.05e-193
Identity = 285/286 (99.65%), Postives = 286/286 (100.00%), Query Frame = 0

Query: 1   MDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVLPSVVPILRYQNGLSNEGMTHEAS 60
           MDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVLPSVVPILRYQNGLSNEGMTHEAS
Sbjct: 144 MDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVLPSVVPILRYQNGLSNEGMTHEAS 203

Query: 61  RFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFSKLKDPNETSKEELPREALKPRDQ 120
           RFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFS+LKDPNETSKEELPREALKPRDQ
Sbjct: 204 RFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFSRLKDPNETSKEELPREALKPRDQ 263

Query: 121 NRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPVPDTKFPEDADESLELNNSMSRLQ 180
           NRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPVPDTKFPEDADESLELNNSMSRLQ
Sbjct: 264 NRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPVPDTKFPEDADESLELNNSMSRLQ 323

Query: 181 KTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA 240
           KTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA
Sbjct: 324 KTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA 383

Query: 241 QEVKILKEKLLELSRRGRASNQVGFPPLFICMVALICIVFGFVLHH 286
           QEVKILKEKLLELSRRGRASNQVGFPPLFICMVALICIVFGFVLHH
Sbjct: 384 QEVKILKEKLLELSRRGRASNQVGFPPLFICMVALICIVFGFVLHH 429

BLAST of Cucsat.G19868.T5 vs. NCBI nr
Match: XP_008455504.1 (PREDICTED: vesicle-associated protein 2-2 isoform X1 [Cucumis melo])

HSP 1 Score: 500 bits (1288), Expect = 3.71e-175
Identity = 263/286 (91.96%), Postives = 271/286 (94.76%), Query Frame = 0

Query: 1   MDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVLPSVVPILRYQNGLSNEGMTHEAS 60
           MDDVMKLNE SGPL DGMDTNE PKLKDQNGASDEVLPS+VPI+RYQNG SNE MT EAS
Sbjct: 144 MDDVMKLNEHSGPLRDGMDTNEIPKLKDQNGASDEVLPSLVPIVRYQNGASNEDMTLEAS 203

Query: 61  RFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFSKLKDPNETSKEELPREALKPRDQ 120
           RFKDDFVALKGERTIEALKPSDQNGAFREEVL HE S+ KDPNETSKEELPRE LKPRDQ
Sbjct: 204 RFKDDFVALKGERTIEALKPSDQNGAFREEVLLHESSRPKDPNETSKEELPREVLKPRDQ 263

Query: 121 NRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPVPDTKFPEDADESLELNNSMSRLQ 180
           NRALKEEELSVESP +KVQNGAL+WGAYGSSHLE+PVPDTKFPED D+SLELNNSM RLQ
Sbjct: 264 NRALKEEELSVESPKRKVQNGALEWGAYGSSHLEDPVPDTKFPEDVDKSLELNNSMPRLQ 323

Query: 181 KTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA 240
           KTAEIKQGTELEP+KLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA
Sbjct: 324 KTAEIKQGTELEPVKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA 383

Query: 241 QEVKILKEKLLELSRRGRASNQVGFPPLFICMVALICIVFGFVLHH 286
           QEVKILKEKLLELSRRGR SNQVGFPPLFICMVALICIV GFVLHH
Sbjct: 384 QEVKILKEKLLELSRRGRTSNQVGFPPLFICMVALICIVLGFVLHH 429

BLAST of Cucsat.G19868.T5 vs. NCBI nr
Match: XP_004139205.1 (vesicle-associated protein 2-2 isoform X2 [Cucumis sativus] >KAE8651570.1 hypothetical protein Csa_019344 [Cucumis sativus])

HSP 1 Score: 459 bits (1182), Expect = 1.44e-159
Identity = 250/286 (87.41%), Postives = 251/286 (87.76%), Query Frame = 0

Query: 1   MDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVLPSVVPILRYQNGLSNEGMTHEAS 60
           MDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDE                         
Sbjct: 144 MDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDE------------------------- 203

Query: 61  RFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFSKLKDPNETSKEELPREALKPRDQ 120
                     GERTIEALKPSDQNGAFREEVLPHEFS+LKDPNETSKEELPREALKPRDQ
Sbjct: 204 ----------GERTIEALKPSDQNGAFREEVLPHEFSRLKDPNETSKEELPREALKPRDQ 263

Query: 121 NRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPVPDTKFPEDADESLELNNSMSRLQ 180
           NRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPVPDTKFPEDADESLELNNSMSRLQ
Sbjct: 264 NRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPVPDTKFPEDADESLELNNSMSRLQ 323

Query: 181 KTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA 240
           KTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA
Sbjct: 324 KTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA 383

Query: 241 QEVKILKEKLLELSRRGRASNQVGFPPLFICMVALICIVFGFVLHH 286
           QEVKILKEKLLELSRRGRASNQVGFPPLFICMVALICIVFGFVLHH
Sbjct: 384 QEVKILKEKLLELSRRGRASNQVGFPPLFICMVALICIVFGFVLHH 394

BLAST of Cucsat.G19868.T5 vs. NCBI nr
Match: XP_038890494.1 (vesicle-associated protein 2-2-like [Benincasa hispida] >XP_038890495.1 vesicle-associated protein 2-2-like [Benincasa hispida])

HSP 1 Score: 444 bits (1142), Expect = 7.84e-153
Identity = 244/296 (82.43%), Postives = 259/296 (87.50%), Query Frame = 0

Query: 1   MDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVLPSVVPILRYQNGLSNEGMTHEAS 60
           MD+VMKLNE SGPL DGMDTNETPKLKDQNGASDEVL S V  L  QNG SNEGMTHEAS
Sbjct: 144 MDEVMKLNEHSGPLRDGMDTNETPKLKDQNGASDEVLLSGVSTLGDQNGPSNEGMTHEAS 203

Query: 61  RFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFSKLKDPNETSKEELPREALKPRDQ 120
           RFKDD+VALKGERTIEALKPSDQNGAFREEVLP E S+LKDPN TSKEE PREALKPRDQ
Sbjct: 204 RFKDDYVALKGERTIEALKPSDQNGAFREEVLPRESSRLKDPNGTSKEEQPREALKPRDQ 263

Query: 121 NRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPVPDT----------KFPEDADESL 180
           NRALKEE +SVESP K+VQNGAL+ GAY +SHLE+ VPDT          KFPED DE+L
Sbjct: 264 NRALKEE-MSVESPKKRVQNGALESGAYETSHLEDSVPDTITIQPPSFVLKFPEDVDETL 323

Query: 181 ELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTIS 240
           ELNN MSR QKTAEIKQ  ELEP+ LK+AEQL++VKDIDEMKSKL+EIELKLGEAQ TIS
Sbjct: 324 ELNNRMSRFQKTAEIKQAAELEPVTLKRAEQLKIVKDIDEMKSKLNEIELKLGEAQVTIS 383

Query: 241 MLSEARRLSAQEVKILKEKLLELSRRGRASNQVGFPPLFICMVALICIVFGFVLHH 286
           MLSEARRLSAQEVK LKEKL+ELSRRGR SNQVGFPPLFICMVALICIVFGFVLHH
Sbjct: 384 MLSEARRLSAQEVKNLKEKLMELSRRGRVSNQVGFPPLFICMVALICIVFGFVLHH 438

BLAST of Cucsat.G19868.T5 vs. NCBI nr
Match: KAA0063645.1 (vesicle-associated protein 2-2 isoform X1 [Cucumis melo var. makuwa] >TYK18380.1 vesicle-associated protein 2-2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 428 bits (1101), Expect = 1.01e-146
Identity = 227/250 (90.80%), Postives = 235/250 (94.00%), Query Frame = 0

Query: 1   MDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVLPSVVPILRYQNGLSNEGMTHEAS 60
           MDDVMKLNE SGPL DGMDTNE PKLKDQNG SDEV PS+VPI+RYQNG SNE MT EAS
Sbjct: 144 MDDVMKLNEHSGPLRDGMDTNEIPKLKDQNGTSDEVFPSLVPIVRYQNGASNEDMTLEAS 203

Query: 61  RFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFSKLKDPNETSKEELPREALKPRDQ 120
           RFKDDFVALKGERTIEALKPSDQNGAFREEVL HE S+ KDPNETSKEELPRE LKPRDQ
Sbjct: 204 RFKDDFVALKGERTIEALKPSDQNGAFREEVLLHESSRPKDPNETSKEELPREVLKPRDQ 263

Query: 121 NRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPVPDTKFPEDADESLELNNSMSRLQ 180
           NRALKEEELSVESP +KVQNGAL+WGAYGSSHLE+PVPDTKFPED D+SLELNNSM RLQ
Sbjct: 264 NRALKEEELSVESPKRKVQNGALEWGAYGSSHLEDPVPDTKFPEDVDKSLELNNSMPRLQ 323

Query: 181 KTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA 240
           KTAEIKQGTELEP+KLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA
Sbjct: 324 KTAEIKQGTELEPVKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA 383

Query: 241 QEVKILKEKL 250
           QEVKILKEKL
Sbjct: 384 QEVKILKEKL 393

BLAST of Cucsat.G19868.T5 vs. ExPASy TrEMBL
Match: A0A0A0LIH5 (MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G013300 PE=4 SV=1)

HSP 1 Score: 546 bits (1408), Expect = 9.95e-194
Identity = 285/286 (99.65%), Postives = 286/286 (100.00%), Query Frame = 0

Query: 1   MDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVLPSVVPILRYQNGLSNEGMTHEAS 60
           MDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVLPSVVPILRYQNGLSNEGMTHEAS
Sbjct: 144 MDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVLPSVVPILRYQNGLSNEGMTHEAS 203

Query: 61  RFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFSKLKDPNETSKEELPREALKPRDQ 120
           RFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFS+LKDPNETSKEELPREALKPRDQ
Sbjct: 204 RFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFSRLKDPNETSKEELPREALKPRDQ 263

Query: 121 NRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPVPDTKFPEDADESLELNNSMSRLQ 180
           NRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPVPDTKFPEDADESLELNNSMSRLQ
Sbjct: 264 NRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPVPDTKFPEDADESLELNNSMSRLQ 323

Query: 181 KTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA 240
           KTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA
Sbjct: 324 KTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA 383

Query: 241 QEVKILKEKLLELSRRGRASNQVGFPPLFICMVALICIVFGFVLHH 286
           QEVKILKEKLLELSRRGRASNQVGFPPLFICMVALICIVFGFVLHH
Sbjct: 384 QEVKILKEKLLELSRRGRASNQVGFPPLFICMVALICIVFGFVLHH 429

BLAST of Cucsat.G19868.T5 vs. ExPASy TrEMBL
Match: A0A1S3C2B8 (vesicle-associated protein 2-2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495652 PE=4 SV=1)

HSP 1 Score: 500 bits (1288), Expect = 1.80e-175
Identity = 263/286 (91.96%), Postives = 271/286 (94.76%), Query Frame = 0

Query: 1   MDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVLPSVVPILRYQNGLSNEGMTHEAS 60
           MDDVMKLNE SGPL DGMDTNE PKLKDQNGASDEVLPS+VPI+RYQNG SNE MT EAS
Sbjct: 144 MDDVMKLNEHSGPLRDGMDTNEIPKLKDQNGASDEVLPSLVPIVRYQNGASNEDMTLEAS 203

Query: 61  RFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFSKLKDPNETSKEELPREALKPRDQ 120
           RFKDDFVALKGERTIEALKPSDQNGAFREEVL HE S+ KDPNETSKEELPRE LKPRDQ
Sbjct: 204 RFKDDFVALKGERTIEALKPSDQNGAFREEVLLHESSRPKDPNETSKEELPREVLKPRDQ 263

Query: 121 NRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPVPDTKFPEDADESLELNNSMSRLQ 180
           NRALKEEELSVESP +KVQNGAL+WGAYGSSHLE+PVPDTKFPED D+SLELNNSM RLQ
Sbjct: 264 NRALKEEELSVESPKRKVQNGALEWGAYGSSHLEDPVPDTKFPEDVDKSLELNNSMPRLQ 323

Query: 181 KTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA 240
           KTAEIKQGTELEP+KLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA
Sbjct: 324 KTAEIKQGTELEPVKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA 383

Query: 241 QEVKILKEKLLELSRRGRASNQVGFPPLFICMVALICIVFGFVLHH 286
           QEVKILKEKLLELSRRGR SNQVGFPPLFICMVALICIV GFVLHH
Sbjct: 384 QEVKILKEKLLELSRRGRTSNQVGFPPLFICMVALICIVLGFVLHH 429

BLAST of Cucsat.G19868.T5 vs. ExPASy TrEMBL
Match: A0A5D3D4C3 (Vesicle-associated protein 2-2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G001030 PE=4 SV=1)

HSP 1 Score: 428 bits (1101), Expect = 4.87e-147
Identity = 227/250 (90.80%), Postives = 235/250 (94.00%), Query Frame = 0

Query: 1   MDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVLPSVVPILRYQNGLSNEGMTHEAS 60
           MDDVMKLNE SGPL DGMDTNE PKLKDQNG SDEV PS+VPI+RYQNG SNE MT EAS
Sbjct: 144 MDDVMKLNEHSGPLRDGMDTNEIPKLKDQNGTSDEVFPSLVPIVRYQNGASNEDMTLEAS 203

Query: 61  RFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFSKLKDPNETSKEELPREALKPRDQ 120
           RFKDDFVALKGERTIEALKPSDQNGAFREEVL HE S+ KDPNETSKEELPRE LKPRDQ
Sbjct: 204 RFKDDFVALKGERTIEALKPSDQNGAFREEVLLHESSRPKDPNETSKEELPREVLKPRDQ 263

Query: 121 NRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPVPDTKFPEDADESLELNNSMSRLQ 180
           NRALKEEELSVESP +KVQNGAL+WGAYGSSHLE+PVPDTKFPED D+SLELNNSM RLQ
Sbjct: 264 NRALKEEELSVESPKRKVQNGALEWGAYGSSHLEDPVPDTKFPEDVDKSLELNNSMPRLQ 323

Query: 181 KTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA 240
           KTAEIKQGTELEP+KLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA
Sbjct: 324 KTAEIKQGTELEPVKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA 383

Query: 241 QEVKILKEKL 250
           QEVKILKEKL
Sbjct: 384 QEVKILKEKL 393

BLAST of Cucsat.G19868.T5 vs. ExPASy TrEMBL
Match: A0A1S3C0N6 (vesicle-associated protein 2-2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495652 PE=4 SV=1)

HSP 1 Score: 424 bits (1091), Expect = 4.71e-146
Identity = 233/286 (81.47%), Postives = 239/286 (83.57%), Query Frame = 0

Query: 1   MDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVLPSVVPILRYQNGLSNEGMTHEAS 60
           MDDVMKLNE SGPL DGMDTNE PKLKDQNGASDE                         
Sbjct: 144 MDDVMKLNEHSGPLRDGMDTNEIPKLKDQNGASDE------------------------- 203

Query: 61  RFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFSKLKDPNETSKEELPREALKPRDQ 120
                     GERTIEALKPSDQNGAFREEVL HE S+ KDPNETSKEELPRE LKPRDQ
Sbjct: 204 ----------GERTIEALKPSDQNGAFREEVLLHESSRPKDPNETSKEELPREVLKPRDQ 263

Query: 121 NRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPVPDTKFPEDADESLELNNSMSRLQ 180
           NRALKEEELSVESP +KVQNGAL+WGAYGSSHLE+PVPDTKFPED D+SLELNNSM RLQ
Sbjct: 264 NRALKEEELSVESPKRKVQNGALEWGAYGSSHLEDPVPDTKFPEDVDKSLELNNSMPRLQ 323

Query: 181 KTAEIKQGTELEPLKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA 240
           KTAEIKQGTELEP+KLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA
Sbjct: 324 KTAEIKQGTELEPVKLKKAEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSA 383

Query: 241 QEVKILKEKLLELSRRGRASNQVGFPPLFICMVALICIVFGFVLHH 286
           QEVKILKEKLLELSRRGR SNQVGFPPLFICMVALICIV GFVLHH
Sbjct: 384 QEVKILKEKLLELSRRGRTSNQVGFPPLFICMVALICIVLGFVLHH 394

BLAST of Cucsat.G19868.T5 vs. ExPASy TrEMBL
Match: A0A6J1G3C7 (vesicle-associated protein 2-2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450409 PE=4 SV=1)

HSP 1 Score: 392 bits (1007), Expect = 3.41e-132
Identity = 225/328 (68.60%), Postives = 250/328 (76.22%), Query Frame = 0

Query: 1   MDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVLPSVVPILRYQNGLSNEGMTHEAS 60
           MD+VMKLNE SGPL DG+ TN+TPKLKDQNG+SDEV  S V  LR QNG SNEGMT EAS
Sbjct: 145 MDEVMKLNEHSGPLRDGIATNKTPKLKDQNGSSDEVRSSGVSSLRDQNGQSNEGMTDEAS 204

Query: 61  RFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFSKLKDPNETSKEELPREALKPRDQ 120
           RFKDDFVALKG RTIEALKPSDQNGA R+EVLPHE S+L D N TSKEELP EALKP DQ
Sbjct: 205 RFKDDFVALKGVRTIEALKPSDQNGALRKEVLPHETSRLMDQNGTSKEELPHEALKPSDQ 264

Query: 121 NRALKEEELSVESPTKKVQNGALKWGAYGSS----------------------------- 180
           N ALKEE LSVE P K+VQNGAL W AY +S                             
Sbjct: 265 NGALKEE-LSVEVPKKRVQNGALDWRAYETSNVENLPPTVVDVQSPRPFERIFPEGGLAY 324

Query: 181 ---HLENPVPDT----------KFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKK 240
              H+E+ VP+T          KFP+  DESLELNNS+SRLQK+AEIK+  EL+ + LKK
Sbjct: 325 KTSHVEDQVPETVAIRPPDFSVKFPDSVDESLELNNSVSRLQKSAEIKKAAELDLVTLKK 384

Query: 241 AEQLQLVKDIDEMKSKLHEIELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGR 286
           AEQL+LVKDI+EMKSKL+EIELKLGEAQGTISML E+RRLSAQEVK LKEKL+ELSR+GR
Sbjct: 385 AEQLKLVKDIEEMKSKLNEIELKLGEAQGTISMLFESRRLSAQEVKHLKEKLMELSRKGR 444

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B9DHD74.8e-0837.17Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
XP_011648789.12.05e-19399.65vesicle-associated protein 2-2 isoform X1 [Cucumis sativus] >XP_011648790.1 vesi... [more]
XP_008455504.13.71e-17591.96PREDICTED: vesicle-associated protein 2-2 isoform X1 [Cucumis melo][more]
XP_004139205.11.44e-15987.41vesicle-associated protein 2-2 isoform X2 [Cucumis sativus] >KAE8651570.1 hypoth... [more]
XP_038890494.17.84e-15382.43vesicle-associated protein 2-2-like [Benincasa hispida] >XP_038890495.1 vesicle-... [more]
KAA0063645.11.01e-14690.80vesicle-associated protein 2-2 isoform X1 [Cucumis melo var. makuwa] >TYK18380.1... [more]
Match NameE-valueIdentityDescription
A0A0A0LIH59.95e-19499.65MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G013300 PE=4 SV... [more]
A0A1S3C2B81.80e-17591.96vesicle-associated protein 2-2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349565... [more]
A0A5D3D4C34.87e-14790.80Vesicle-associated protein 2-2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3C0N64.71e-14681.47vesicle-associated protein 2-2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349565... [more]
A0A6J1G3C73.41e-13268.60vesicle-associated protein 2-2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 208..228
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 99..136
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..34
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 99..133
NoneNo IPR availablePANTHERPTHR10809:SF45VESICLE-ASSOCIATED PROTEIN 2-2coord: 92..285
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 92..285

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G19868Cucsat.G19868gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G19868.T5.E1Cucsat.G19868.T5.E1exon
Cucsat.G19868.T5.E2Cucsat.G19868.T5.E2exon
Cucsat.G19868.T5.E3Cucsat.G19868.T5.E3exon
Cucsat.G19868.T5.E4Cucsat.G19868.T5.E4exon
Cucsat.G19868.T5.E5Cucsat.G19868.T5.E5exon
Cucsat.G19868.T5.E6Cucsat.G19868.T5.E6exon
Cucsat.G19868.T5.E7Cucsat.G19868.T5.E7exon
Cucsat.G19868.T5.E8Cucsat.G19868.T5.E8exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G19868.T5.C1Cucsat.G19868.T5.C1CDS
Cucsat.G19868.T5.C2Cucsat.G19868.T5.C2CDS
Cucsat.G19868.T5.C3Cucsat.G19868.T5.C3CDS
Cucsat.G19868.T5.C4Cucsat.G19868.T5.C4CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G19868.T5Cucsat.G19868.T5-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0061817 endoplasmic reticulum-plasma membrane tethering
biological_process GO:0090158 endoplasmic reticulum membrane organization
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane