Cucsat.G16573.T3 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G16573.T3
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionRibonuclease
Locationctg24: 884872 .. 887643 (+)
RNA-Seq ExpressionCucsat.G16573.T3
SyntenyCucsat.G16573.T3
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTAAACCTATAGCAAGAAAATTTGTGCTTGCACGCGGGAACAACCTAAAAGAAACACTAAATAGAAGAAAAAGCGGGAGGAGATTAGCTGATAAACTGCAATGTCTTCTCAAGATTCACTCCCAAAATGGACTTCCAAGCCTTGTGTAATGGGCATAGATGAAGCTGGTCGAGGCCCTGTTTTAGGTTTTCTATTTTCCTATTTGTTTTATTCCATTTCTGTCTCTTTTAACTCCGTTTCGATCATCATATTTTGGGATACTTTTTTTATTTCAGGTCCAATGGTGTATGGATGTTTGTACTGCGCCAGTTCCTATTTGAAACAACTTTCGTCTTTGAATTTTGCAGGTTTGCCTAATTTATGCTTCTATTCATTTTCTTTTTGACTGTTAATCATCATTCCCTGCTTCGTTTACTTCTCTTCCATGTAGACTCGAAAACTTTGAAAGAAGAGAAGAGGGAGGAATTGTTTGAAGATTTGAAGGCTAATGAGTCAATTGGATGGGCTGTTGATGTCATTGATCCTCGAGAACTTTCTTCGAAGATGCTAAATAAGTCAGTCTAAATGGTTTATGAATTCTTGCATTTGCACAAACACTCTACTTTCCACTACACCTTTTGAATTCGAAGGTACAAAGAATGGGATTCAGCTGAAATCTCCTTTTTTCTGGAACTTAAGTTGACTGATTAAGATGAAATCATTTAATTTCTAGTAATTCTTTAAACTAAGACAATGAAACTTATCTTCATTCAGAGTTAGAGTCAGCAAAAGACTGAACGTGGATAATGATGCTCTCCCTCCCTCCCCCTTCTAGTTTTGTTAATTTATGCACATCAAAGGGCAACTATGGCTTGGACTAAAGTTTTGTTTTTTTTCTCCTGCCTACTTTATCAAGAATATTACTTGTAAAGTTTATGAAATATGATGCTATCATAATTAATCATATGAGAATTATCACACCTGGGTTTTTGTTTCTGATTTCTTTTTTCTTTATTGCTCAACAGAGTAAAGATCAACCTAAATGAAATATCACATGACTCTGCGATGGGTCTGGTCACCAAGGTTCTAAGCATGGGAGTTCTTTTAACTGAGGTAACTCTTTCCCTTGTTTCTTCATTGACTGATGAAAAGAAACTAAACTTGTTAAGAATACAGTCCCGATAGAGATAGAAGAGGAAATAGCATTTGAAGAGGAAATAGCATTTGAAGAGGAGATAGCATATGGAATCTTGAACTTCATTTGTTATCATGTCATAACTTTTAACTTTTTTGGAGGGCAAAATATAAAAATTTCAAACTACAGATTTTTAATGCTTTGTTAATTATAGATCTAGACCAAAGTCCACAAGGAGGTAAAATGAACAAGTTTTATTTCTTGATTACAGTAGCACGGCGTGACACATTCTTTATTAGATAGATGTGTAAAAATTAAGTTTTGGATGTCATGTTCTTGATTTTTTTTTCAAGTCCATAATAGGGAGGGAAAGAAAATCCCCATTCAGCAAATCTCATCTTTATTTTCAGGTTTATGTGGATACTGTTGGAGATCCTGAAAAGTACAGGATCAAGTTGTCTGAAAAATTTCCTAATATAAAATTTGTAGTTGCAAAGAAAGCTGATAGCTTGTATCCTGTTGTCAGTGGGGCAAGCATAGTCGCAAAGGTAATGCTAATGCTTCCTTTTAGGATATAGCATTCTAACTGTTTCCCACACTTCAACATCAATGTGACTTGTAGGTCACAAGAGATCGTGCATTGCGGGAATGGGCATTGGAGGAAACAGCTGAAAACATGCATAGAAACTTTGGTTCTGGATACCCTGGAGGTCTATTTCATATGCTAAATATTTTTTGCAGCATCCTATAATCTGATTTTCGTTGCACAGGGTGATATCAATAATGCTCTTATGATTTAATCTATATAGATCCAACAACTAAATGTTGGTTAGAAGATCACAAGCACTCGGTATTTGGGTTCCCTACACTAGTCCGCTTCAGTTGGGGTACCTGCACTTCATATTTCAAAGACGTTGTTGAAGTATCATGGTTAGTTTTTACTCTGCCTTGTAAGAATTGTAGCTCTTTGCAGAATTAGCTCATTAAGCCATATAGAATTTCTTTTATGTTTGCCACGGTATGGAAAGGACAGGCTTTGTTCTAGCCTATAGTTTGTTTGCTGCACTCCACTTCTATTGTAGAATAGTTGGACAACAATTTAGGTTGGTTCCTGATGTCAACTTCTTGATACACTTTTATTGTAGAACTTTGGTATTAAGTTCTTTCGATTGTGTATTCACATTGATGTCATATTGTTATATTATTGTATTTACATGCTTGATTTACAGGGAGTCGGATAAAGTGGATGAAGATGGGGCAACGGGTGGGAGTGGTAAGAAACAATTAAGATTAAGCAATGTTGGCATCACCACATCCAAGAGAAAGAGTGAAGAAATTGAATCAAGTGGCAAAGGACGGTGCAAGTTTTTCCAGGCTAGAAAACTTGAACAACTCTCTCGTTTTTGAATGTTTGATTACATTCTCCAATTGTTAAAGATTAACTAAGTAGCTTATAACCATTACTTTGGCTGTGTACCAACTGGTTATTGTGCTTGGAAAACTCTTTCTTAATGATGTGTAACAGTTACTTTGTAATCGCCTAAAAGTAGAATGCTGTTATACATTTCTCTTTTATCTTTTTTCCCACTTTTTCAAATGGCTCCTAATTTGAACATTTGGAGAAGTGTTTTTAGGTCTAAAAATCACTTCTCCCTC

Coding sequence (CDS)

ATGGGTCTGGTCACCAAGGTTCTAAGCATGGGAGTTCTTTTAACTGAGGTTTATGTGGATACTGTTGGAGATCCTGAAAAGTACAGGATCAAGTTGTCTGAAAAATTTCCTAATATAAAATTTGTAGTTGCAAAGAAAGCTGATAGCTTGTATCCTGTTGTCAGTGGGGCAAGCATAGTCGCAAAGGTCACAAGAGATCGTGCATTGCGGGAATGGGCATTGGAGGAAACAGCTGAAAACATGCATAGAAACTTTGGTTCTGGATACCCTGGAGATCCAACAACTAAATGTTGGTTAGAAGATCACAAGCACTCGGTATTTGGGTTCCCTACACTAGTCCGCTTCAGTTGGGGTACCTGCACTTCATATTTCAAAGACGTTGTTGAAGTATCATGGGAGTCGGATAAAGTGGATGAAGATGGGGCAACGGGTGGGAGTGGTAAGAAACAATTAAGATTAAGCAATGTTGGCATCACCACATCCAAGAGAAAGAGTGAAGAAATTGAATCAAGTGGCAAAGGACGGTGCAAGTTTTTCCAGGCTAGAAAACTTGAACAACTCTCTCGTTTTTGA

Protein sequence

MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIVAKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESSGKGRCKFFQARKLEQLSRF
Homology
BLAST of Cucsat.G16573.T3 vs. ExPASy Swiss-Prot
Match: Q9SEZ6 (Ribonuclease H2 subunit A OS=Arabidopsis thaliana OX=3702 GN=At2g25100 PE=2 SV=2)

HSP 1 Score: 285.4 bits (729), Expect = 4.7e-76
Identity = 135/190 (71.05%), Postives = 166/190 (87.37%), Query Frame = 0

Query: 1   MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV 60
           MGL+ +VL MGVLLTE Y+DTVGDP+KYRIKLSE+FP+IKFVV+KKADSL+P+VSGASIV
Sbjct: 108 MGLIKRVLDMGVLLTEAYLDTVGDPDKYRIKLSERFPSIKFVVSKKADSLFPIVSGASIV 167

Query: 61  AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 120
           AKVTRDRAL+EW +EET E+++RNFGSGYPGDP TK WL  HKHSVFGFP+LVRFSWGTC
Sbjct: 168 AKVTRDRALKEWLVEETGEDINRNFGSGYPGDPETKAWLVQHKHSVFGFPSLVRFSWGTC 227

Query: 121 TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESSGKGRCKFFQ 180
           T++ K  VEV+WE+D+ +E G  G S K+Q +LS+ G  T +++SEEIESSGKGRCKF Q
Sbjct: 228 TTHLKGEVEVAWEADENEESG-NGSSSKRQAKLSSFGFKTCEKRSEEIESSGKGRCKFLQ 287

Query: 181 ARKLEQLSRF 191
           ARK++QL++F
Sbjct: 288 ARKIQQLTQF 296

BLAST of Cucsat.G16573.T3 vs. ExPASy Swiss-Prot
Match: Q5U209 (Ribonuclease H2 subunit A OS=Rattus norvegicus OX=10116 GN=Rnaseh2a PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 2.6e-34
Identity = 67/140 (47.86%), Postives = 91/140 (65.00%), Query Frame = 0

Query: 2   GLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIVA 61
           GL+   +   V +T+V+VDTVG PE Y+ +L ++FP I+  V  KADSL+PVVS ASI+A
Sbjct: 124 GLIQHAMDQNVKVTQVFVDTVGMPETYQARLQQRFPGIEVTVKAKADSLFPVVSAASIIA 183

Query: 62  KVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCT 121
           KV RD+A++ W   E+ + +  ++GSGYP DP TK WL  H   VFGFP  VRFSW T  
Sbjct: 184 KVARDQAVKNWQFVESLQGLDSDYGSGYPNDPKTKAWLRKHVDPVFGFPQFVRFSWSTAQ 243

Query: 122 SYF-KDVVEVSWESDKVDED 141
           +   K+   V+WE    +ED
Sbjct: 244 AILEKEAESVTWEDSAAEED 263

BLAST of Cucsat.G16573.T3 vs. ExPASy Swiss-Prot
Match: Q9VPP5 (Ribonuclease H2 subunit A OS=Drosophila melanogaster OX=7227 GN=CG13690 PE=2 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 4.4e-34
Identity = 88/190 (46.32%), Postives = 110/190 (57.89%), Query Frame = 0

Query: 1   MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV 60
           MGL+ + +  GV + EVYVDTVG PEKY+ KL ++FP+ K  VAKKADS YP+VS ASI 
Sbjct: 168 MGLIQQAIDAGVNIAEVYVDTVGPPEKYQEKLLKRFPSFKITVAKKADSTYPIVSAASIC 227

Query: 61  AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 120
           AKVTRD AL+ W+  E        FGSGYPGDP T+ +L ++   VFGFP LVRFSW T 
Sbjct: 228 AKVTRDHALKVWSFPEGLVIKDNEFGSGYPGDPVTRRFLTEYIDLVFGFPRLVRFSWSTA 287

Query: 121 TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESSGKGRCKFFQ 180
            +   D      E D+ D +       K  L     G T    KS EI    +  C+FF+
Sbjct: 288 ENALADKA-YDMEFDEPDSEKPKYAGTK--LTKFFKGTT----KSGEII---REECRFFK 347

Query: 181 ARKLEQLSRF 191
            R LE +  F
Sbjct: 348 QRHLESVMEF 347

BLAST of Cucsat.G16573.T3 vs. ExPASy Swiss-Prot
Match: Q9CWY8 (Ribonuclease H2 subunit A OS=Mus musculus OX=10090 GN=Rnaseh2a PE=1 SV=2)

HSP 1 Score: 144.8 bits (364), Expect = 9.8e-34
Identity = 68/140 (48.57%), Postives = 90/140 (64.29%), Query Frame = 0

Query: 2   GLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIVA 61
           GL+   L   V +T+V+VDTVG PE Y+ +L + FP I+  V  KADSL+PVVS ASI A
Sbjct: 124 GLIQYALDQNVNVTQVFVDTVGMPETYQARLQQHFPGIEVTVKAKADSLFPVVSAASIFA 183

Query: 62  KVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCT 121
           KV RD+A++ W   E  +++  ++GSGYP DP TK WL  H   VFGFP  VRFSW T  
Sbjct: 184 KVARDKAVKNWQFVENLQDLDSDYGSGYPNDPKTKAWLRKHVDPVFGFPQFVRFSWSTAQ 243

Query: 122 SYF-KDVVEVSWESDKVDED 141
           +   K+  +V WE  + +ED
Sbjct: 244 AILEKEAEDVIWEDSEAEED 263

BLAST of Cucsat.G16573.T3 vs. ExPASy Swiss-Prot
Match: O75792 (Ribonuclease H2 subunit A OS=Homo sapiens OX=9606 GN=RNASEH2A PE=1 SV=2)

HSP 1 Score: 143.7 bits (361), Expect = 2.2e-33
Identity = 68/133 (51.13%), Postives = 90/133 (67.67%), Query Frame = 0

Query: 2   GLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIVA 61
           GL+   L  GV +T+V+VDTVG PE Y+ +L + FP I+  V  KAD+LYPVVS ASI A
Sbjct: 123 GLIQYALDQGVNVTQVFVDTVGMPETYQARLQQSFPGIEVTVKAKADALYPVVSAASICA 182

Query: 62  KVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC- 121
           KV RD+A+++W   E  +++  ++GSGYP DP TK WL++H   VFGFP  VRFSW T  
Sbjct: 183 KVARDQAVKKWQFVEKLQDLDTDYGSGYPNDPKTKAWLKEHVEPVFGFPQFVRFSWRTAQ 242

Query: 122 TSYFKDVVEVSWE 134
           T   K+  +V WE
Sbjct: 243 TILEKEAEDVIWE 255

BLAST of Cucsat.G16573.T3 vs. NCBI nr
Match: XP_004152096.1 (ribonuclease H2 subunit A [Cucumis sativus] >KGN53090.1 hypothetical protein Csa_014407 [Cucumis sativus])

HSP 1 Score: 384 bits (987), Expect = 4.77e-133
Identity = 190/190 (100.00%), Postives = 190/190 (100.00%), Query Frame = 0

Query: 1   MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV 60
           MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV
Sbjct: 108 MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV 167

Query: 61  AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 120
           AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC
Sbjct: 168 AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 227

Query: 121 TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESSGKGRCKFFQ 180
           TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESSGKGRCKFFQ
Sbjct: 228 TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESSGKGRCKFFQ 287

Query: 181 ARKLEQLSRF 190
           ARKLEQLSRF
Sbjct: 288 ARKLEQLSRF 297

BLAST of Cucsat.G16573.T3 vs. NCBI nr
Match: XP_008453984.1 (PREDICTED: ribonuclease H2 subunit A isoform X2 [Cucumis melo])

HSP 1 Score: 381 bits (978), Expect = 2.07e-132
Identity = 188/190 (98.95%), Postives = 189/190 (99.47%), Query Frame = 0

Query: 1   MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV 60
           MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV
Sbjct: 60  MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV 119

Query: 61  AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 120
           AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC
Sbjct: 120 AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 179

Query: 121 TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESSGKGRCKFFQ 180
           TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIES+ KGRCKFFQ
Sbjct: 180 TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQ 239

Query: 181 ARKLEQLSRF 190
           ARKLEQLSRF
Sbjct: 240 ARKLEQLSRF 249

BLAST of Cucsat.G16573.T3 vs. NCBI nr
Match: XP_008453982.1 (PREDICTED: ribonuclease H2 subunit A isoform X1 [Cucumis melo] >KAA0044595.1 ribonuclease H2 subunit A isoform X1 [Cucumis melo var. makuwa] >TYK16989.1 ribonuclease H2 subunit A isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 381 bits (978), Expect = 1.12e-131
Identity = 188/190 (98.95%), Postives = 189/190 (99.47%), Query Frame = 0

Query: 1   MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV 60
           MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV
Sbjct: 108 MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV 167

Query: 61  AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 120
           AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC
Sbjct: 168 AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 227

Query: 121 TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESSGKGRCKFFQ 180
           TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIES+ KGRCKFFQ
Sbjct: 228 TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQ 287

Query: 181 ARKLEQLSRF 190
           ARKLEQLSRF
Sbjct: 288 ARKLEQLSRF 297

BLAST of Cucsat.G16573.T3 vs. NCBI nr
Match: KAG6582048.1 (Ribonuclease H2 subunit A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 373 bits (958), Expect = 1.24e-128
Identity = 181/190 (95.26%), Postives = 187/190 (98.42%), Query Frame = 0

Query: 1   MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV 60
           MGLVTKVL MGVLLTEVYVDTVGDPEKYRIKLSEKFPN+KFVVAKKADSLYPVVSGASIV
Sbjct: 108 MGLVTKVLGMGVLLTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIV 167

Query: 61  AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 120
           AKVTRDR LR+WALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC
Sbjct: 168 AKVTRDRVLRDWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 227

Query: 121 TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESSGKGRCKFFQ 180
           TSYFKD+VEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIES+ KGRCKFFQ
Sbjct: 228 TSYFKDIVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQ 287

Query: 181 ARKLEQLSRF 190
           ARKLEQL++F
Sbjct: 288 ARKLEQLTQF 297

BLAST of Cucsat.G16573.T3 vs. NCBI nr
Match: KAG7018477.1 (Ribonuclease H2 subunit A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 373 bits (957), Expect = 1.43e-128
Identity = 181/190 (95.26%), Postives = 186/190 (97.89%), Query Frame = 0

Query: 1   MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV 60
           MGLVTKVL MGVLLTEVYVDTVGDPEKYRIKLSEKFPN+KFVVAKKADSLYPVVSGASIV
Sbjct: 102 MGLVTKVLGMGVLLTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIV 161

Query: 61  AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 120
           AKVTRDR LR+WALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC
Sbjct: 162 AKVTRDRVLRDWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 221

Query: 121 TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESSGKGRCKFFQ 180
           TSYFKD+VEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIES+ KGRCKFFQ
Sbjct: 222 TSYFKDIVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQ 281

Query: 181 ARKLEQLSRF 190
           ARKLEQL+ F
Sbjct: 282 ARKLEQLTHF 291

BLAST of Cucsat.G16573.T3 vs. ExPASy TrEMBL
Match: A0A0A0KTZ4 (Ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_4G015800 PE=3 SV=1)

HSP 1 Score: 384 bits (987), Expect = 2.31e-133
Identity = 190/190 (100.00%), Postives = 190/190 (100.00%), Query Frame = 0

Query: 1   MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV 60
           MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV
Sbjct: 108 MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV 167

Query: 61  AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 120
           AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC
Sbjct: 168 AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 227

Query: 121 TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESSGKGRCKFFQ 180
           TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESSGKGRCKFFQ
Sbjct: 228 TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESSGKGRCKFFQ 287

Query: 181 ARKLEQLSRF 190
           ARKLEQLSRF
Sbjct: 288 ARKLEQLSRF 297

BLAST of Cucsat.G16573.T3 vs. ExPASy TrEMBL
Match: A0A1S3BYD1 (Ribonuclease OS=Cucumis melo OX=3656 GN=LOC103494542 PE=3 SV=1)

HSP 1 Score: 381 bits (978), Expect = 1.00e-132
Identity = 188/190 (98.95%), Postives = 189/190 (99.47%), Query Frame = 0

Query: 1   MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV 60
           MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV
Sbjct: 60  MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV 119

Query: 61  AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 120
           AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC
Sbjct: 120 AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 179

Query: 121 TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESSGKGRCKFFQ 180
           TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIES+ KGRCKFFQ
Sbjct: 180 TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQ 239

Query: 181 ARKLEQLSRF 190
           ARKLEQLSRF
Sbjct: 240 ARKLEQLSRF 249

BLAST of Cucsat.G16573.T3 vs. ExPASy TrEMBL
Match: A0A5A7TT92 (Ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001310 PE=3 SV=1)

HSP 1 Score: 381 bits (978), Expect = 5.42e-132
Identity = 188/190 (98.95%), Postives = 189/190 (99.47%), Query Frame = 0

Query: 1   MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV 60
           MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV
Sbjct: 108 MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV 167

Query: 61  AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 120
           AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC
Sbjct: 168 AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 227

Query: 121 TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESSGKGRCKFFQ 180
           TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIES+ KGRCKFFQ
Sbjct: 228 TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQ 287

Query: 181 ARKLEQLSRF 190
           ARKLEQLSRF
Sbjct: 288 ARKLEQLSRF 297

BLAST of Cucsat.G16573.T3 vs. ExPASy TrEMBL
Match: A0A1S3BXP1 (Ribonuclease OS=Cucumis melo OX=3656 GN=LOC103494542 PE=3 SV=1)

HSP 1 Score: 381 bits (978), Expect = 5.42e-132
Identity = 188/190 (98.95%), Postives = 189/190 (99.47%), Query Frame = 0

Query: 1   MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV 60
           MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV
Sbjct: 108 MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV 167

Query: 61  AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 120
           AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC
Sbjct: 168 AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 227

Query: 121 TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESSGKGRCKFFQ 180
           TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIES+ KGRCKFFQ
Sbjct: 228 TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQ 287

Query: 181 ARKLEQLSRF 190
           ARKLEQLSRF
Sbjct: 288 ARKLEQLSRF 297

BLAST of Cucsat.G16573.T3 vs. ExPASy TrEMBL
Match: A0A6J1GTR5 (Ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111457459 PE=3 SV=1)

HSP 1 Score: 373 bits (957), Expect = 8.54e-129
Identity = 181/190 (95.26%), Postives = 186/190 (97.89%), Query Frame = 0

Query: 1   MGLVTKVLSMGVLLTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIV 60
           MGLVTKVL MGVLLTEVYVDTVGDPEKYRIKLSEKFPN+KFVVAKKADSLYPVVSGASIV
Sbjct: 108 MGLVTKVLGMGVLLTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIV 167

Query: 61  AKVTRDRALREWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 120
           AKVTRDR LR+WALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC
Sbjct: 168 AKVTRDRVLRDWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC 227

Query: 121 TSYFKDVVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESSGKGRCKFFQ 180
           TSYFKD+VEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIES+ KGRCKFFQ
Sbjct: 228 TSYFKDIVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQ 287

Query: 181 ARKLEQLSRF 190
           ARKLEQL+ F
Sbjct: 288 ARKLEQLTHF 297

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SEZ64.7e-7671.05Ribonuclease H2 subunit A OS=Arabidopsis thaliana OX=3702 GN=At2g25100 PE=2 SV=2[more]
Q5U2092.6e-3447.86Ribonuclease H2 subunit A OS=Rattus norvegicus OX=10116 GN=Rnaseh2a PE=2 SV=1[more]
Q9VPP54.4e-3446.32Ribonuclease H2 subunit A OS=Drosophila melanogaster OX=7227 GN=CG13690 PE=2 SV=... [more]
Q9CWY89.8e-3448.57Ribonuclease H2 subunit A OS=Mus musculus OX=10090 GN=Rnaseh2a PE=1 SV=2[more]
O757922.2e-3351.13Ribonuclease H2 subunit A OS=Homo sapiens OX=9606 GN=RNASEH2A PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_004152096.14.77e-133100.00ribonuclease H2 subunit A [Cucumis sativus] >KGN53090.1 hypothetical protein Csa... [more]
XP_008453984.12.07e-13298.95PREDICTED: ribonuclease H2 subunit A isoform X2 [Cucumis melo][more]
XP_008453982.11.12e-13198.95PREDICTED: ribonuclease H2 subunit A isoform X1 [Cucumis melo] >KAA0044595.1 rib... [more]
KAG6582048.11.24e-12895.26Ribonuclease H2 subunit A, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7018477.11.43e-12895.26Ribonuclease H2 subunit A [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A0A0KTZ42.31e-133100.00Ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_4G015800 PE=3 SV=1[more]
A0A1S3BYD11.00e-13298.95Ribonuclease OS=Cucumis melo OX=3656 GN=LOC103494542 PE=3 SV=1[more]
A0A5A7TT925.42e-13298.95Ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001310 ... [more]
A0A1S3BXP15.42e-13298.95Ribonuclease OS=Cucumis melo OX=3656 GN=LOC103494542 PE=3 SV=1[more]
A0A6J1GTR58.54e-12995.26Ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111457459 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..79
e-value: 1.9E-24
score: 88.4
IPR024567Ribonuclease HII/HIII domainPFAMPF01351RNase_HIIcoord: 4..120
e-value: 2.4E-23
score: 83.0
IPR023160Ribonuclease HII, helix-loop-helix cap domain superfamilyGENE3D1.10.10.460Ribonuclease hii. Domain 2coord: 80..127
e-value: 3.4E-20
score: 73.4
IPR001352Ribonuclease HII/HIIIPANTHERPTHR10954RIBONUCLEASE H2 SUBUNIT Acoord: 1..190
NoneNo IPR availablePANTHERPTHR10954:SF19RIBONUCLEASEcoord: 1..190
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 7..126

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G16573Cucsat.G16573gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G16573.T3.E1Cucsat.G16573.T3.E1exon
Cucsat.G16573.T3.E2Cucsat.G16573.T3.E2exon
Cucsat.G16573.T3.E3Cucsat.G16573.T3.E3exon
Cucsat.G16573.T3.E4Cucsat.G16573.T3.E4exon
Cucsat.G16573.T3.E5Cucsat.G16573.T3.E5exon
Cucsat.G16573.T3.E6Cucsat.G16573.T3.E6exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G16573.T3.C1Cucsat.G16573.T3.C1CDS
Cucsat.G16573.T3.C2Cucsat.G16573.T3.C2CDS
Cucsat.G16573.T3.C3Cucsat.G16573.T3.C3CDS
Cucsat.G16573.T3.C4Cucsat.G16573.T3.C4CDS
Cucsat.G16573.T3.C5Cucsat.G16573.T3.C5CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G16573.T3Cucsat.G16573.T3-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043137 DNA replication, removal of RNA primer
biological_process GO:0006298 mismatch repair
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0032299 ribonuclease H2 complex
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003723 RNA binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016740 transferase activity