Cucsat.G12789.T4 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G12789.T4
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Tubulin alpha chain
Location	ctg1838: 4393936 .. 4396791 (-)
RNA-Seq Expression	Cucsat.G12789.T4
Synteny	Cucsat.G12789.T4

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTCGTTTATGTTTTTCTTTGGAATTTTGGCTGCTTTTTCCCGATCTCTTGATTTGTTGAGGGATTTGAGTCTTTATGAGAGATCAAAGGAGGAATATGGAAATGGGTTTTAATTTTCATTTTAATTGGATGTACCTCTATGGATCTCCTTCTTGAACTTGAAACTAATTGAAAACTCGCGTCGAGCAATAACCCAATTGATTTGGTAATCCTTTGCTCCGGGTGTTTCAAATCTAATGGCCAATGTTTGGTGTTTGTTAAGGCTAGATAGTTTGTGGTGATGTCTCTGTTGAGGAAATGAATTGTTGGGGTCTTCTTGGAGGACCTTCTCAATGAATTAATTAATTAATGTTGTTTTTTTATGAAATTTAAGAATGGATCTTTTGTTTTATGTAGTTTCTTTGATAGTGGCATAAGAAGTCGTCGTTTGATTTTATGTTCCCTTTCAAGCTATTGGTATGGCTAATTGCCGTGAATGATCTCTTATTCTAACGATGATTTGTTACCTGTGTATGCTGCAGTGACACATCCGTCGGTGTTGCACATGATGCTTTCAACACGTTTTTCAGCGAAACTGGTTCGGGCAAGCATGTTCCTAGAGCAATATTTGTGGATCTGGAACCAACTGTTATCGATGAAGTGAGGACCGGAACATACCGTCAGCTTTTCCATCCGGAACAGCTCATATCTGGTAAAGAAGATGCTGCAAATAATTTTGCGAGAGGACATTATACAGGTACTTGTGTTTGTGTTCCTTCAAGTTTTTCTATAGTTTCCTTTTTCACTATTGTCATTTTTTACTAAATCTACATTATTGCATCATTCCAGTGGGCAGGGAAATAGTTGATCTATGCCTCGACCGAGTGCGAAAATTAGCCGACAATTGCACTGGTTTACAAGGGTTCTTGGTATTCAATGCTGTTGGTGGTGGCACTGGATCTGGATTGGGGTCATTACTTTTGGAACGCTTGTCTGTGGATTACGGAAAGAAGTCAAAGCTTGGATTTTCCATATATCCTTCTCCACAGGTAAGACACAAATTATAAAGGCTTTGGTAGTATACACCAAACATTTTTCATAAATTATGGTGACAAAACACTATGAAAAGGCCACTGACCTTTTGTCAAGATTTTTCGTATTAAAAGGTTTCTTATCTGGAAAAAATTTGTTTCTGATGAGAAATACTGAGTTGACGTGTATTCTTCTCCACAGATTTTTCCTCATTCTCACTGTCCATCATAAATCTAAATGCTACCTTCTTTGTGTGCAGGTATCTACTGCTGTGGTAGAACCCTACAACAGTGTGCTTTCCACTCATTCTCTGCTAGAACACACAGATGTTGCTGTTCTTCTCGATAATGAAGCCATTTACGATATCTGTAGGAGATCATTGGATATTGAAAGACCAACTTACACCAACTTGAACCGATTGATATCCCAAGTTATCTCTTCTTTAACCACTTCCTTGCGGTTTGATGGTGCTATCAACGTGGATATAACTGAGTTTCAAACTAACCTTGTACCTTATCCACGTATCCATTTCATGCTCTCTTCTTATGCTCCTGTGATTTCTTCTGCAAAAGCTTACCATGAGCAGCTATCCGTCCCTGAAATTACCAATGCTGTATTTGATCCCTCAAGCATGATGGTCAAGTGCGATCCCAGGCACGGAAAGTACATGGCATGCTGTTTGATGTATAGAGGAGATGTTGTACCCAAGGATGTGAATGCTGCTGTTGCTACTATCAAAACAAAGCGAACAGTTCAATTTGTCGACTGGTGAGTTAACTGCTCTGTATTGTTAGATTATGAAGCCAATTATACTTTTTTAACTCTGCAGAAATAAAAAAATTAATTTGGCTCAGCATATGATTGAAATCAAGCTATAAAGTATGATGCACATCCATAAGTAACCATCCTCAAAGGATGCTAGTTTAGTTTGTCAACTTTAGAAAAGGTTCTTGCAAATCATAGGGCATACGTAATTTTCTAAATGTTTTGCTATGAATTAAAGTTATGCAATGGGAATTTTCCAGGTGTCCTACTGGCTTCAAATGCGGTATTAACTATCAGCCACCAAGTGTTGTTCCTGGAGGCGATCTTGCCAAGGTGCAGCGAGCTGTCTGTATGATAAGCAATAGCACAGCCGTTGCCGAAGTATTTTCAAGAATTGACCACAAATTCGATCTTATGTATGCTAAGCGCGCATTTGTCCATTGGTATGTGGGGGAGGGCATGGAGGAAGGCGAATTCTCCGAAGCCCGTGAGGATCTCGCTGCACTAGAAAAGGACTACGAGGAAGTCGGAGCAGAAGATGTCGATGAAGAGGAAGAAGGGGATGATTATTGAGCTCGTGAATAATAATATACCCTGTGTATCTTTTTTTTCTTTTCGTTGTTTTTGTGTTTGTTTCTTGTTAAGTCAAGTTGTGCAATGTTTCATACATGGTGATATATCTTGAAGGTGTGGTTTATGTTCTTTTGTATGATTCTGGGGTCTGTCATTCTATTGATTTCGAAATTTTACAGTGTTTAGAAACTACTTTGCAAAGTTTATTATGAGGAGACGAGGTTTCTGTAAAGATCTTTCCGAGGAGTTCCCTTTGAAACTTTCTCGGATTTTCACCTCATGGAATCCACAATTCGTATTGCGAAGGCCAAGTGTTTTTGGCATTGCTTCTAAGTAATTTTGGATAACAATATGTACTTCTGATTTAATTATTTCCTGTTGAAGAAATATGTAATAAGCCCTTATCTGAAGGCTTTGTTTCGATTATTTTTTTTGAATGATTTTTTAAGGATTTGAGAATTTCTAAATTTTGAACATTTGAAATCAATTAGAATGGTTAATATTAT

Coding sequence (CDS)

ATGCTCTCTTCTTATGCTCCTGTGATTTCTTCTGCAAAAGCTTACCATGAGCAGCTATCCGTCCCTGAAATTACCAATGCTGTATTTGATCCCTCAAGCATGATGGTCAAGTGCGATCCCAGGCACGGAAAGTACATGGCATGCTGTTTGATGTATAGAGGAGATGTTGTACCCAAGGATGTGAATGCTGCTGTTGCTACTATCAAAACAAAGCGAACAGTTCAATTTGTCGACTGGTGTCCTACTGGCTTCAAATGCGGTATTAACTATCAGCCACCAAGTGTTGTTCCTGGAGGCGATCTTGCCAAGGTGCAGCGAGCTGTCTGTATGATAAGCAATAGCACAGCCGTTGCCGAAGTATTTTCAAGAATTGACCACAAATTCGATCTTATGTATGCTAAGCGCGCATTTGTCCATTGGTATGTGGGGGAGGGCATGGAGGAAGGCGAATTCTCCGAAGCCCGTGAGGATCTCGCTGCACTAGAAAAGGACTACGAGGAAGTCGGAGCAGAAGATGTCGATGAAGAGGAAGAAGGGGATGATTATTGA

Protein sequence

MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGDDY

Homology

[Close]

BLAST of Cucsat.G12789.T4 vs. ExPASy Swiss-Prot
Match: Q43473 (Tubulin alpha-1 chain OS=Hordeum vulgare OX=4513 GN=TUBA1 PE=2 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 5.1e-96
Identity = 173/183 (94.54%), Postives = 178/183 (97.27%), Query Frame = 0


Query: 1   MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 60
           MLSSYAPVIS+ KAYHEQLSVPEITNAVF+PSSMM KCDPRHGKYMACCLMYRGDVVPKD
Sbjct: 268 MLSSYAPVISAEKAYHEQLSVPEITNAVFEPSSMMAKCDPRHGKYMACCLMYRGDVVPKD 327

Query: 61  VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 120
           VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISN+TAVAEV
Sbjct: 328 VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNNTAVAEV 387

Query: 121 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEE-EEG 180
           FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAE  D+E +EG
Sbjct: 388 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEGADDEGDEG 447

Query: 181 DDY 183
           DDY
Sbjct: 448 DDY 450

[Close]

BLAST of Cucsat.G12789.T4 vs. ExPASy Swiss-Prot
Match: Q02245 (Tubulin alpha-5 chain OS=Zea mays OX=4577 GN=TUBA5 PE=2 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 5.1e-96
Identity = 173/183 (94.54%), Postives = 178/183 (97.27%), Query Frame = 0


Query: 1   MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 60
           MLSSYAPVIS+ KAYHEQLSVPEITNAVF+PSSMM KCDPRHGKYMACCLMYRGDVVPKD
Sbjct: 268 MLSSYAPVISAEKAYHEQLSVPEITNAVFEPSSMMAKCDPRHGKYMACCLMYRGDVVPKD 327

Query: 61  VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 120
           VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISN+TAVAEV
Sbjct: 328 VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNNTAVAEV 387

Query: 121 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEE-EEG 180
           FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAE  D+E +EG
Sbjct: 388 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEGADDEGDEG 447

Query: 181 DDY 183
           DDY
Sbjct: 448 DDY 450

[Close]

BLAST of Cucsat.G12789.T4 vs. ExPASy Swiss-Prot
Match: P33627 (Tubulin alpha-6 chain OS=Zea mays OX=4577 GN=TUBA6 PE=2 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 5.1e-96
Identity = 173/183 (94.54%), Postives = 178/183 (97.27%), Query Frame = 0


Query: 1   MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 60
           MLSSYAPVIS+ KAYHEQLSVPEITNAVF+PSSMM KCDPRHGKYMACCLMYRGDVVPKD
Sbjct: 268 MLSSYAPVISAEKAYHEQLSVPEITNAVFEPSSMMAKCDPRHGKYMACCLMYRGDVVPKD 327

Query: 61  VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 120
           VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISN+TAVAEV
Sbjct: 328 VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNNTAVAEV 387

Query: 121 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEE-EEG 180
           FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAE  D+E +EG
Sbjct: 388 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEGADDEGDEG 447

Query: 181 DDY 183
           DDY
Sbjct: 448 DDY 450

[Close]

BLAST of Cucsat.G12789.T4 vs. ExPASy Swiss-Prot
Match: O22349 (Tubulin alpha-3 chain OS=Eleusine indica OX=29674 GN=TUBA3 PE=2 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 1.5e-95
Identity = 172/183 (93.99%), Postives = 178/183 (97.27%), Query Frame = 0


Query: 1   MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 60
           MLSSYAPVIS+ KAYHEQLSVPEITNAVF+PSSMM KCDPRHGKYMACCLMYRGDVVPKD
Sbjct: 268 MLSSYAPVISAEKAYHEQLSVPEITNAVFEPSSMMAKCDPRHGKYMACCLMYRGDVVPKD 327

Query: 61  VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 120
           VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISN+TAVAEV
Sbjct: 328 VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNNTAVAEV 387

Query: 121 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEE-EEG 180
           FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAE  D+E +EG
Sbjct: 388 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEGADDEGDEG 447

Query: 181 DDY 183
           +DY
Sbjct: 448 EDY 450

[Close]

BLAST of Cucsat.G12789.T4 vs. ExPASy Swiss-Prot
Match: P28752 (Tubulin alpha-1 chain OS=Oryza sativa subsp. japonica OX=39947 GN=TUBA1 PE=1 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 3.3e-95
Identity = 171/183 (93.44%), Postives = 178/183 (97.27%), Query Frame = 0


Query: 1   MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 60
           MLSSYAPVIS+ KAYHEQLSVPEITNAVF+PSSMM KCDPRHGKYMACCLMYRGDVVPKD
Sbjct: 268 MLSSYAPVISAEKAYHEQLSVPEITNAVFEPSSMMAKCDPRHGKYMACCLMYRGDVVPKD 327

Query: 61  VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 120
           VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISN+TAVAEV
Sbjct: 328 VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNNTAVAEV 387

Query: 121 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEE-EEG 180
           FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAE  D+E ++G
Sbjct: 388 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEGADDENDDG 447

Query: 181 DDY 183
           +DY
Sbjct: 448 EDY 450

[Close]

BLAST of Cucsat.G12789.T4 vs. NCBI nr
Match: XP_004141134.1 (tubulin alpha-6 chain [Cucumis sativus] >KGN59749.1 hypothetical protein Csa_001017 [Cucumis sativus])

HSP 1 Score: 367 bits (941), Expect = 5.57e-124
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 1   MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 60
           MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD
Sbjct: 268 MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 327

Query: 61  VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 120
           VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV
Sbjct: 328 VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 387

Query: 121 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGD 180
           FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGD
Sbjct: 388 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGD 447

Query: 181 DY 182
           DY
Sbjct: 448 DY 449

[Close]

BLAST of Cucsat.G12789.T4 vs. NCBI nr
Match: XP_022930580.1 (tubulin alpha-3 chain-like [Cucurbita moschata] >XP_022980617.1 tubulin alpha-3 chain-like [Cucurbita maxima] >XP_023526047.1 tubulin alpha-3 chain-like [Cucurbita pepo subsp. pepo] >KAG6581659.1 Tubulin alpha-3 chain, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018153.1 Tubulin alpha-3 chain [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 365 bits (937), Expect = 2.25e-123
Identity = 181/182 (99.45%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 1   MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 60
           MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD
Sbjct: 268 MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 327

Query: 61  VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 120
           VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV
Sbjct: 328 VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 387

Query: 121 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGD 180
           FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEG+
Sbjct: 388 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGE 447

Query: 181 DY 182
           DY
Sbjct: 448 DY 449

[Close]

BLAST of Cucsat.G12789.T4 vs. NCBI nr
Match: XP_008464962.1 (PREDICTED: tubulin alpha-3 chain-like [Cucumis melo] >XP_022932678.1 tubulin alpha-3 chain-like [Cucurbita moschata] >XP_022972128.1 tubulin alpha-3 chain-like [Cucurbita maxima] >XP_023539676.1 tubulin alpha-3 chain-like [Cucurbita pepo subsp. pepo] >KAA0057190.1 tubulin alpha-3 chain-like [Cucumis melo var. makuwa] >TYK18771.1 tubulin alpha-3 chain-like [Cucumis melo var. makuwa])

HSP 1 Score: 365 bits (937), Expect = 2.25e-123
Identity = 181/182 (99.45%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 1   MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 60
           MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD
Sbjct: 268 MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 327

Query: 61  VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 120
           VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV
Sbjct: 328 VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 387

Query: 121 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGD 180
           FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEG+
Sbjct: 388 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGE 447

Query: 181 DY 182
           DY
Sbjct: 448 DY 449

[Close]

BLAST of Cucsat.G12789.T4 vs. NCBI nr
Match: XP_038904017.1 (tubulin alpha-5 chain-like [Benincasa hispida])

HSP 1 Score: 365 bits (937), Expect = 2.25e-123
Identity = 181/182 (99.45%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 1   MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 60
           MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD
Sbjct: 268 MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 327

Query: 61  VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 120
           VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV
Sbjct: 328 VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 387

Query: 121 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGD 180
           FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEG+
Sbjct: 388 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGE 447

Query: 181 DY 182
           DY
Sbjct: 448 DY 449

[Close]

BLAST of Cucsat.G12789.T4 vs. NCBI nr
Match: XP_022155952.1 (tubulin alpha-3 chain-like [Momordica charantia])

HSP 1 Score: 365 bits (936), Expect = 3.19e-123
Identity = 180/182 (98.90%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 1   MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 60
           MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD
Sbjct: 268 MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 327

Query: 61  VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 120
           VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV
Sbjct: 328 VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 387

Query: 121 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGD 180
           FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAED+DEEEEG+
Sbjct: 388 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDIDEEEEGE 447

Query: 181 DY 182
           DY
Sbjct: 448 DY 449

[Close]

BLAST of Cucsat.G12789.T4 vs. ExPASy TrEMBL
Match: A0A0A0LFM5 (Tubulin alpha chain OS=Cucumis sativus OX=3659 GN=Csa_3G842710 PE=3 SV=1)

HSP 1 Score: 367 bits (941), Expect = 2.70e-124
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 1   MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 60
           MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD
Sbjct: 268 MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 327

Query: 61  VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 120
           VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV
Sbjct: 328 VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 387

Query: 121 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGD 180
           FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGD
Sbjct: 388 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGD 447

Query: 181 DY 182
           DY
Sbjct: 448 DY 449

[Close]

BLAST of Cucsat.G12789.T4 vs. ExPASy TrEMBL
Match: A0A5D3D5F0 (Tubulin alpha chain OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold257G00780 PE=3 SV=1)

HSP 1 Score: 365 bits (937), Expect = 1.09e-123
Identity = 181/182 (99.45%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 1   MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 60
           MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD
Sbjct: 268 MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 327

Query: 61  VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 120
           VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV
Sbjct: 328 VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 387

Query: 121 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGD 180
           FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEG+
Sbjct: 388 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGE 447

Query: 181 DY 182
           DY
Sbjct: 448 DY 449

[Close]

BLAST of Cucsat.G12789.T4 vs. ExPASy TrEMBL
Match: A0A6J1I8Z7 (Tubulin alpha chain OS=Cucurbita maxima OX=3661 GN=LOC111470761 PE=3 SV=1)

HSP 1 Score: 365 bits (937), Expect = 1.09e-123
Identity = 181/182 (99.45%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 1   MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 60
           MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD
Sbjct: 268 MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 327

Query: 61  VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 120
           VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV
Sbjct: 328 VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 387

Query: 121 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGD 180
           FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEG+
Sbjct: 388 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGE 447

Query: 181 DY 182
           DY
Sbjct: 448 DY 449

[Close]

BLAST of Cucsat.G12789.T4 vs. ExPASy TrEMBL
Match: A0A6J1IRT1 (Tubulin alpha chain OS=Cucurbita maxima OX=3661 GN=LOC111479935 PE=3 SV=1)

HSP 1 Score: 365 bits (937), Expect = 1.09e-123
Identity = 181/182 (99.45%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 1   MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 60
           MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD
Sbjct: 268 MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 327

Query: 61  VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 120
           VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV
Sbjct: 328 VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 387

Query: 121 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGD 180
           FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEG+
Sbjct: 388 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGE 447

Query: 181 DY 182
           DY
Sbjct: 448 DY 449

[Close]

BLAST of Cucsat.G12789.T4 vs. ExPASy TrEMBL
Match: A0A6J1EX84 (Tubulin alpha chain OS=Cucurbita moschata OX=3662 GN=LOC111436987 PE=3 SV=1)

HSP 1 Score: 365 bits (937), Expect = 1.09e-123
Identity = 181/182 (99.45%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 1   MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 60
           MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD
Sbjct: 268 MLSSYAPVISSAKAYHEQLSVPEITNAVFDPSSMMVKCDPRHGKYMACCLMYRGDVVPKD 327

Query: 61  VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 120
           VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV
Sbjct: 328 VNAAVATIKTKRTVQFVDWCPTGFKCGINYQPPSVVPGGDLAKVQRAVCMISNSTAVAEV 387

Query: 121 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGD 180
           FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEG+
Sbjct: 388 FSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEDVDEEEEGE 447

Query: 181 DY 182
           DY
Sbjct: 448 DY 449

The following BLAST results are available for this feature:

BLAST of Cucsat.G12789.T4 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43473	5.1e-96	94.54	Tubulin alpha-1 chain OS=Hordeum vulgare OX=4513 GN=TUBA1 PE=2 SV=1	[more]
Q02245	5.1e-96	94.54	Tubulin alpha-5 chain OS=Zea mays OX=4577 GN=TUBA5 PE=2 SV=1	[more]
P33627	5.1e-96	94.54	Tubulin alpha-6 chain OS=Zea mays OX=4577 GN=TUBA6 PE=2 SV=1	[more]
O22349	1.5e-95	93.99	Tubulin alpha-3 chain OS=Eleusine indica OX=29674 GN=TUBA3 PE=2 SV=1	[more]
P28752	3.3e-95	93.44	Tubulin alpha-1 chain OS=Oryza sativa subsp. japonica OX=39947 GN=TUBA1 PE=1 SV=...	[more]

BLAST of Cucsat.G12789.T4 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141134.1	5.57e-124	100.00	tubulin alpha-6 chain [Cucumis sativus] >KGN59749.1 hypothetical protein Csa_001...	[more]
XP_022930580.1	2.25e-123	99.45	tubulin alpha-3 chain-like [Cucurbita moschata] >XP_022980617.1 tubulin alpha-3 ...	[more]
XP_008464962.1	2.25e-123	99.45	PREDICTED: tubulin alpha-3 chain-like [Cucumis melo] >XP_022932678.1 tubulin alp...	[more]
XP_038904017.1	2.25e-123	99.45	tubulin alpha-5 chain-like [Benincasa hispida]	[more]
XP_022155952.1	3.19e-123	98.90	tubulin alpha-3 chain-like [Momordica charantia]	[more]

BLAST of Cucsat.G12789.T4 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LFM5	2.70e-124	100.00	Tubulin alpha chain OS=Cucumis sativus OX=3659 GN=Csa_3G842710 PE=3 SV=1	[more]
A0A5D3D5F0	1.09e-123	99.45	Tubulin alpha chain OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold257G...	[more]
A0A6J1I8Z7	1.09e-123	99.45	Tubulin alpha chain OS=Cucurbita maxima OX=3661 GN=LOC111470761 PE=3 SV=1	[more]
A0A6J1IRT1	1.09e-123	99.45	Tubulin alpha chain OS=Cucurbita maxima OX=3661 GN=LOC111479935 PE=3 SV=1	[more]
A0A6J1EX84	1.09e-123	99.45	Tubulin alpha chain OS=Cucurbita moschata OX=3662 GN=LOC111436987 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 151..178
None	No IPR available	PANTHER	PTHR11588:SF373	TUBULIN ALPHA CHAIN	coord: 1..180
IPR002452	Alpha tubulin	PRINTS	PR01162	ALPHATUBULIN	coord: 7..22 score: 75.26 coord: 118..134 score: 83.59 coord: 155..171 score: 90.81 coord: 54..75 score: 87.24 coord: 93..112 score: 86.34 coord: 80..93 score: 93.1
IPR018316	Tubulin/FtsZ, 2-layer sandwich domain	SMART	SM00865	Tubulin_C_4	coord: 1..126 e-value: 3.0E-31 score: 119.8
IPR018316	Tubulin/FtsZ, 2-layer sandwich domain	PFAM	PF03953	Tubulin_C	coord: 2..125 e-value: 1.4E-47 score: 161.0
IPR023123	Tubulin, C-terminal	GENE3D	1.10.287.600	Helix hairpin bin	coord: 117..169 e-value: 3.2E-32 score: 111.9
IPR037103	Tubulin/FtsZ-like, C-terminal domain	GENE3D	3.30.1330.20		coord: 2..116 e-value: 1.9E-60 score: 203.8
IPR000217	Tubulin	PANTHER	PTHR11588	TUBULIN	coord: 1..180
IPR008280	Tubulin/FtsZ, C-terminal	SUPERFAMILY	55307	Tubulin C-terminal domain-like	coord: 2..170

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G12789	Cucsat.G12789	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12789.T4.E1	Cucsat.G12789.T4.E1	exon
Cucsat.G12789.T4.E2	Cucsat.G12789.T4.E2	exon
Cucsat.G12789.T4.E3	Cucsat.G12789.T4.E3	exon
Cucsat.G12789.T4.E4	Cucsat.G12789.T4.E4	exon
Cucsat.G12789.T4.E5	Cucsat.G12789.T4.E5	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12789.T4.C2	Cucsat.G12789.T4.C2	CDS
Cucsat.G12789.T4.C1	Cucsat.G12789.T4.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G12789.T4	Cucsat.G12789.T4-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007017	microtubule-based process
biological_process	GO:0000226	microtubule cytoskeleton organization
biological_process	GO:0000278	mitotic cell cycle
cellular_component	GO:0005874	microtubule
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005525	GTP binding
molecular_function	GO:0005200	structural constituent of cytoskeleton
molecular_function	GO:0003924	GTPase activity