Tan0021194 (gene) Snake gourd v1

Overview
NameTan0021194
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionMethyltransf_11 domain-containing protein
LocationLG08: 3158207 .. 3162510 (+)
RNA-Seq ExpressionTan0021194
SyntenyTan0021194
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGTCATTAAAACCAGCTCCTTAATCTTGCTGCTGATTCCTGAAAGGGGAAGAAGAACCAGAGGTCCGAGAAGCTTCCAATCCATTTTCATGGCGGTAGCTATTTTCTTTCCTCCTCAAACTTCATACAACAATCGCCATTATCTTTCTCGCGATTTCCGATTTCGGTCTCCTCTTCGCTTGTCCACTCGTCGTTGTGTCTCCTCTACCGTTCGGGCGAGCTCGACGCTCGTTCTTGAGTCGGTAAGCTCCACCTTAACTTTCTTGTTCCAATTTTCGTTTATAATTCGAATTTTTGATGTTATTGCTGTTAAAACGAATCTCTGTTGTGCGTTGAAGTTGAATTGTATTGTGATATGGAGTTGACGATTCGGACATGTATGTTTCGGGATTGACAATAGGCTGAATTTTGCAATAATTTGGAGAGGAGTATGTAGATGCTACCCCGTGTTTGGCTTTTTAGGGAAATTATTGTTTGAGGAATTTGTAATTTCAACTCAACTTCGAACTGTTTCATTTAGTCGAATTTGGAGTTTCTATTATTATTATTATTTGTTTCCCTCAGGATTTAGGCATTCAACAGGATCAGAATTCAAAAATTGACGTGTTTTCATGCCCTGTTTGTTTTGAGCCACTGTTGAGAAAAGGCCCGCCAGGTTTTAACTTGTAAGTTGACTTAATGTGATGTATTCCATCTTCTGATATTTAACTTGTTGCATTTTATGATATTTCGACCCGAGTGAAGTGGAAAATTTGCAGGCCAGCTATTTATAGGTCTGGTTTCAAGTGTAGAAGATGCAATAAGTCATACACTAGCAAAAACATCTTTTTGGATCTCACAGTTACTTCTGGAATGAAGGAATATGTTGAAGTCAAACCTGGGGGAACTGAGCTGTTTCGGTATCTTTCTGATATTTTCTTTCATTTTTTCTTCTAAATGTTTCATTCACTTCTAAGTTTTCTGATTTTGCTTGCTTCATCTTAAGTGTTAAGTTGATGTCACCCATTACAACTTGTCTAACGAAAGGAAGTGGTACTGTGTTATTGCCATTTTTTCCCCTGAAGGAGACAATTCTCTATTGCTTATGGTTTTTGTACTTTGTAGGAGTCCACTCGTTTCATTTCTGTATGAAAGAGGATGGCGACAAAACTTTAACCGAAGTGGTTTCCCCGGCCTCGATGAAGAAGTATGCTTCCTCTCAATCATAACACTACCATTCAAACGTACGAATATTTTATATTGATATTTTGATGCCGTTCAAAAGATTACTCGTGGAGAAAAACAAGTGTTATCGTATTGTTCATGAGTTGCCTTGGCATTGGATATATGGCCTTTTCATTTCCTTGAATAATGTGAATATGGGCTGATATTTTTCTTTAAATATATGCCATCGCAGTTTAAAATGGCCATGGAGTATTTTAAATCTGTTGAAGGTGGTCTTTTAGTGGATGCAAGCTGTGGTAGTGGTTTGTTCTCCAGAAAATTTGCAAAATCTGGGTCTTTTTCAGGTGTTATTGCACTTGATTTTTCTGAGAATATGCTCCTCCAATGTTATGATTTCATAAAGAAAGATGCTGCGCTTTTGAACAGGTGATTTTATTTTATGACTTTTATACTCTTGTTTAATTAATTCCTTAGAACACAACTGATATACCCTGCTAGCTTGTGTATTAAGTCCTCTCAACTTGCAAGAAAAGTGAAGGCTATCCTAATTGGTATATCTTCTTTTGGAAAATATGCAGTAATCTTGCTCTTGTGAGGGCAGATATCTCTAGGCTTCCCTTTTCATCAGGATCAGTGGATGCTGTTCATGCTGGTGCAGCATTGCATTGCTGGCCATCTCCTTCCAATGCTGTAAGCATTTTGTCTTGTAGGATCATATTATATCATTTGATTTCCTACCATTAGTTGTAATCCGCTGAATGCAACCAGGTTATAGAATATCACTGTCAACTTCATCCCATTATTTTCTTGATCAAAAAAAAAAACTTCATCCCATTATTTTCACTATAGAGTTTCTCTTCTCAACCAGTGTATGTGATTGAATTCATTTAAAGTTTCTCTTTCCATGCTCGCTACAATTCCTCGTGGTACTTAGTCCATTGAAAGGAAGCGATCAAAGCTTTCCCTTCATAATAGTTATATGCACTCAATTCACAAAATAATAATATGTTATTGTGTCTGAAACAACGGCAGGTTCCACTGGGCAATTGGTTTTCTGATATATCTTCTTGACTAATGCATTTCTTTTAGGAAGTTCTATTTGAAATCTTTAGGAATTAGCTCCTGAGTATTTTTGTATGTTTTTAGCAACGAGGCATCCATTTATGATATTTTCAGCTCAGATTCTCATTGTTCAGATGATAAGGTCAATATTTTTCAACTTCTTTAGTAATGGTTTCTTTTAACTCTTCTTATCAGATTGCTGAGATCACTCGTATAATGCGGAGTGGTGGAGTATTTGTAGGAACTACTTTTTTGCGGTACACTTCATCCACTCCCTGGTATATAAGATTTTCTAGAGAGGTATACCCTTAATCTATTCTCTCTCATTTTTGCACTTCTAGATATCACTAGTCAGAAGCAGTTATGACAAACATCTGTGTTATCTGATTGATATTTCTGTTCCAGAGTCATGAATTTGGTAATCATGCATTTATATACACGAAATCCGTCCTTGCATATTTTCCTTTCCAATTAACTATGGTTAATTTCTTGATTCTGTTACTTCAAAATGACAAAAAATGTATTTGGGCACCCTGTAAGGTGGTATAAACAAACTAGCTTCCTTCATGGTTAATTGAACTTCTTGCTGGCTTATATTTTGTCATTTTCATTGGACTGAGTATTGGTATTTATTAGAGTCCATAAATCTTCTGGAACCATACATAAACATGAACTAACATAGTTTGTTTTACTTTCTCAAGTTTATAGAAGATCTCCATTTATCAGTTCGGACAACTTTTTCTCATGTAAAGATTGATATCTGTGTGGAATGTGTTCAAAACTCAGTTTCTTTTGTGCTTGCGCCACCATTTTTTTGGGTTGCTTTTGGATGAGAGGCCAACTTTTATGGAGATAAATGAAAGGATACAGAGGAGGCCGAAAAAGCCATGCCAAAAGCGGAGATTAAATAACTCACAATAAACTTACAAAAAGAACTCATCTAGTAAAGATAACTATGAAAAAAATTTAAACTAAAGTACTAAAATGTGTCAAATCCCAAACACATCATCCCACGACCTACATAATCCAACCAACATTCCCCTATTTCTTTCTAGTCAAACAAACTACAGTATGATGAAGAAGCTAACTTGTTGGAAAGCACAACCTTCTAGGAAAAGGAGGATCCAAGACGACGCCCTCCATCATCCTGTAGATACCGACCTCTAAACTACAAATAATCGATACCAACTGATCCAAAATAAACATCTAAATTGCTACTTACCATATTATGATAAATAATTGTATTGAGGCGACGAGTGAATCTGTGATAAGTATTAGATAACCTGAGGTTCCATCTCAAAACCAATTGGTAATGAGAAGAGTAGCTCATGTATCTTATAAATTGTGAGGTCACCTTACATTTTTTCAATGTAGTATCCTCAACATGCCCTCTCAAGATTGTGCCTGTTTGGATTCACCATTAAGTTGGAATTTTGATCGAATACCCGTTTGGGTTTTATGGGCTATGATACCATATTAGATAACAGGAAGTTCCATCTCAAAACCAATTGAGAATGGGAAGAATAACCATGTATATTATAAATCGTGAGATCATTCTACATTTTTCTAGTGAGAGATCCTCAACAATAAGTTCTCCTCTTTGAACAAACTGCATTAATGTTTTGTATATGGAGATGACACTCACGAGATTCAAGGTTCCTCTAATTCTGAAGGATGCAATGATTTTCACATTTTTTCTATATTATCAAGATGGCATTTGAGGCTATAAGTTTTGTGTGCTGCAGCGTGCTTTTCAGAATTTTGGCTACTTAACAGAGGAAGAAATAGAGGATTTGTGCAAATCCTGCGGTCTGATAAACTATTCAAGCAAAATTCAGCAATCTTTCATTATGTTCTCTGCTCAGAAGCCTTGAAAGTAAGTTCTCAGTTTCATTCTCATTTTCATTGTGATGGGTATGTTCATAATCAAACTCTATAGAATTATATTTGATAATTGAATACCATACCATGTAAATTGTTGAAACTGAAAAGTATAATTAATTGAATTGAATAGAATTATTTGTTAATATACTCCAACTGTTTCAAGATTGAGAAGAAACGTGTAAATTGTAATAATGC

mRNA sequence

CGTCATTAAAACCAGCTCCTTAATCTTGCTGCTGATTCCTGAAAGGGGAAGAAGAACCAGAGGTCCGAGAAGCTTCCAATCCATTTTCATGGCGGTAGCTATTTTCTTTCCTCCTCAAACTTCATACAACAATCGCCATTATCTTTCTCGCGATTTCCGATTTCGGTCTCCTCTTCGCTTGTCCACTCGTCGTTGTGTCTCCTCTACCGTTCGGGCGAGCTCGACGCTCGTTCTTGAGTCGGATTTAGGCATTCAACAGGATCAGAATTCAAAAATTGACGTGTTTTCATGCCCTGTTTGTTTTGAGCCACTGTTGAGAAAAGGCCCGCCAGGTTTTAACTTGCCAGCTATTTATAGGTCTGGTTTCAAGTGTAGAAGATGCAATAAGTCATACACTAGCAAAAACATCTTTTTGGATCTCACAGTTACTTCTGGAATGAAGGAATATGTTGAAGTCAAACCTGGGGGAACTGAGCTGTTTCGGAGTCCACTCGTTTCATTTCTGTATGAAAGAGGATGGCGACAAAACTTTAACCGAAGTGGTTTCCCCGGCCTCGATGAAGAATTTAAAATGGCCATGGAGTATTTTAAATCTGTTGAAGGTGGTCTTTTAGTGGATGCAAGCTGTGGTAGTGGTTTGTTCTCCAGAAAATTTGCAAAATCTGGGTCTTTTTCAGGTGTTATTGCACTTGATTTTTCTGAGAATATGCTCCTCCAATGTTATGATTTCATAAAGAAAGATGCTGCGCTTTTGAACAGTAATCTTGCTCTTGTGAGGGCAGATATCTCTAGGCTTCCCTTTTCATCAGGATCAGTGGATGCTGTTCATGCTGGTGCAGCATTGCATTGCTGGCCATCTCCTTCCAATGCTATTGCTGAGATCACTCGTATAATGCGGAGTGGTGGAGTATTTGTAGGAACTACTTTTTTGCGGTACACTTCATCCACTCCCTGGTATATAAGATTTTCTAGAGAGCGTGCTTTTCAGAATTTTGGCTACTTAACAGAGGAAGAAATAGAGGATTTGTGCAAATCCTGCGGTCTGATAAACTATTCAAGCAAAATTCAGCAATCTTTCATTATGTTCTCTGCTCAGAAGCCTTGAAAGTAAGTTCTCAGTTTCATTCTCATTTTCATTGTGATGGGTATGTTCATAATCAAACTCTATAGAATTATATTTGATAATTGAATACCATACCATGTAAATTGTTGAAACTGAAAAGTATAATTAATTGAATTGAATAGAATTATTTGTTAATATACTCCAACTGTTTCAAGATTGAGAAGAAACGTGTAAATTGTAATAATGC

Coding sequence (CDS)

ATGGCGGTAGCTATTTTCTTTCCTCCTCAAACTTCATACAACAATCGCCATTATCTTTCTCGCGATTTCCGATTTCGGTCTCCTCTTCGCTTGTCCACTCGTCGTTGTGTCTCCTCTACCGTTCGGGCGAGCTCGACGCTCGTTCTTGAGTCGGATTTAGGCATTCAACAGGATCAGAATTCAAAAATTGACGTGTTTTCATGCCCTGTTTGTTTTGAGCCACTGTTGAGAAAAGGCCCGCCAGGTTTTAACTTGCCAGCTATTTATAGGTCTGGTTTCAAGTGTAGAAGATGCAATAAGTCATACACTAGCAAAAACATCTTTTTGGATCTCACAGTTACTTCTGGAATGAAGGAATATGTTGAAGTCAAACCTGGGGGAACTGAGCTGTTTCGGAGTCCACTCGTTTCATTTCTGTATGAAAGAGGATGGCGACAAAACTTTAACCGAAGTGGTTTCCCCGGCCTCGATGAAGAATTTAAAATGGCCATGGAGTATTTTAAATCTGTTGAAGGTGGTCTTTTAGTGGATGCAAGCTGTGGTAGTGGTTTGTTCTCCAGAAAATTTGCAAAATCTGGGTCTTTTTCAGGTGTTATTGCACTTGATTTTTCTGAGAATATGCTCCTCCAATGTTATGATTTCATAAAGAAAGATGCTGCGCTTTTGAACAGTAATCTTGCTCTTGTGAGGGCAGATATCTCTAGGCTTCCCTTTTCATCAGGATCAGTGGATGCTGTTCATGCTGGTGCAGCATTGCATTGCTGGCCATCTCCTTCCAATGCTATTGCTGAGATCACTCGTATAATGCGGAGTGGTGGAGTATTTGTAGGAACTACTTTTTTGCGGTACACTTCATCCACTCCCTGGTATATAAGATTTTCTAGAGAGCGTGCTTTTCAGAATTTTGGCTACTTAACAGAGGAAGAAATAGAGGATTTGTGCAAATCCTGCGGTCTGATAAACTATTCAAGCAAAATTCAGCAATCTTTCATTATGTTCTCTGCTCAGAAGCCTTGA

Protein sequence

MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQNSKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEYVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASCGSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSSGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRERAFQNFGYLTEEEIEDLCKSCGLINYSSKIQQSFIMFSAQKP
Homology
BLAST of Tan0021194 vs. ExPASy Swiss-Prot
Match: Q0WPT7 (Uncharacterized methyltransferase At2g41040, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g41040 PE=1 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 2.8e-116
Identity = 212/332 (63.86%), Postives = 255/332 (76.81%), Query Frame = 0

Query: 14  NNRHYLSRDFRFRSP-LRLSTRRCVSSTVRASSTLVLESDLGIQQDQNSKID-----VFS 73
           N   + S D   +SP LR S    V S   +++   +     I +++  KI+     VF+
Sbjct: 23  NRSSFFSSD--SQSPLLRFSASTSVRSRFPSAAISAVAPKSDINKNETPKIEIEETQVFA 82

Query: 74  CPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEYVEVKPGG 133
           CPVC+EPL+RKGP G NL AIYRSGFKC +CNK+Y+SK+ +LDLTVT+ + +Y EVKP  
Sbjct: 83  CPVCYEPLMRKGPSGINLQAIYRSGFKCGQCNKTYSSKDEYLDLTVTADLDDYNEVKPIT 142

Query: 134 TELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASCGSGLFSR 193
           TELFRSPLVSFLYERGWRQ F RSGFPG DEEF+MA EYFK  EGGLLVD SCGSGLFSR
Sbjct: 143 TELFRSPLVSFLYERGWRQAFKRSGFPGPDEEFRMAEEYFKEAEGGLLVDVSCGSGLFSR 202

Query: 194 KFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNS-NLALVRADISRLPFSSGSVDAV 253
           KFA+SG +SGVIALD+SENML QC +FIK D    NS N+A+VRAD+SRLPF SGSVDAV
Sbjct: 203 KFAQSGKYSGVIALDYSENMLRQCKEFIKNDNTFDNSTNIAVVRADVSRLPFPSGSVDAV 262

Query: 254 HAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRERAFQNFGYLT 313
           HAGAALHCWPSP+NAIAEI R++RSGGVFVGTTFLRY+ STPW IR  + R  Q++ YL 
Sbjct: 263 HAGAALHCWPSPTNAIAEICRVLRSGGVFVGTTFLRYSPSTPWIIRPFQSRILQSYNYLM 322

Query: 314 EEEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 339
           ++EI+D+C SCGL +Y   IQ SFIMF+A+KP
Sbjct: 323 QDEIKDVCTSCGLTDYEDYIQDSFIMFTARKP 352

BLAST of Tan0021194 vs. ExPASy Swiss-Prot
Match: Q8LBV4 (Uncharacterized methyltransferase At1g78140, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g78140 PE=1 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 9.8e-77
Identity = 157/323 (48.61%), Postives = 203/323 (62.85%), Query Frame = 0

Query: 37  VSSTVRASSTLVLESDLGIQQDQNSKID--------------VFSCPVCFEPLL-RKGPP 96
           VS +  ASS     + + ++ + NS +D              + +CP+C+  L     P 
Sbjct: 38  VSRSAHASS-----ASVSVETNSNSNVDFVIEKKDKNRGEKKILACPICYNSLAWISQPN 97

Query: 97  GFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEYVEVKPGGTELFRSPLVSFLYE 156
           G    A      +C  C +SY+     LDL V SG K Y E  P  TELFR+PLVSFLYE
Sbjct: 98  GLIESAASGIQVQCNTCKRSYSGNETHLDLAVASGSKRYSEPMPLSTELFRTPLVSFLYE 157

Query: 157 RGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASCGSGLFSRKFAKSGSFSGVIAL 216
           RGWRQNF   GFPG ++EF+MA  Y K V GG ++DASCGSG+FSR F +S  FS VIAL
Sbjct: 158 RGWRQNFIWGGFPGPEKEFEMAKAYLKPVLGGNIIDASCGSGMFSRLFTRSDLFSLVIAL 217

Query: 217 DFSENMLLQCYDFIKKDAALLN-SNLALVRADISRLPFSSGSVDAVHAGAALHCWPSPSN 276
           D+SENML QCY+ + K+    N   L LVRADI+RLPF SGSVDAVHAGAALHCWPSPS+
Sbjct: 218 DYSENMLRQCYELLNKEENFPNKEKLVLVRADIARLPFLSGSVDAVHAGAALHCWPSPSS 277

Query: 277 AIAEITRIMRSGGVFVGTTFLRYTSSTPW--YIRFSRERAFQNFG---YLTEEEIEDLCK 336
           A+AEI+R++R GGVFV TTF+ Y     +  +++  R+   +  G   +L E E+ED+CK
Sbjct: 278 AVAEISRVLRPGGVFVATTFI-YDGPFSFIPFLKNLRQEIMRYSGSHIFLNERELEDICK 337

Query: 337 SCGLINYSSKIQQSFIMFSAQKP 339
           +CGL+N++      FIM SA KP
Sbjct: 338 ACGLVNFTRVRNGPFIMLSATKP 354

BLAST of Tan0021194 vs. ExPASy Swiss-Prot
Match: Q5HP74 (Demethylmenaquinone methyltransferase OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) OX=176279 GN=menG PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 9.0e-06
Identity = 39/117 (33.33%), Postives = 57/117 (48.72%), Query Frame = 0

Query: 161 KMAMEYFKSVEGGLLVDASCGSGLFSRKFAKS-GSFSGVIALDFSENMLLQCYDFIKKDA 220
           K  M      +G   +D  CG+  ++   +++ GS   V  LDFSENML    +  K+  
Sbjct: 39  KHVMSTMNVQKGSKALDVCCGTADWTIALSEAVGSKGQVTGLDFSENML----EVGKQKT 98

Query: 221 ALLNSNLALVRADISRLPFSSGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFV 277
           A L  N+ LV  D   LPF   S D V  G  L   P   +A+ E+ R+++ GG+ V
Sbjct: 99  ASL-ENIQLVHGDAMNLPFDDNSFDYVTIGFGLRNVPDYLSALKEMHRVLKPGGMVV 150

BLAST of Tan0021194 vs. ExPASy Swiss-Prot
Match: Q8CSH9 (Demethylmenaquinone methyltransferase OS=Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) OX=176280 GN=menG PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 9.0e-06
Identity = 39/117 (33.33%), Postives = 57/117 (48.72%), Query Frame = 0

Query: 161 KMAMEYFKSVEGGLLVDASCGSGLFSRKFAKS-GSFSGVIALDFSENMLLQCYDFIKKDA 220
           K  M      +G   +D  CG+  ++   +++ GS   V  LDFSENML    +  K+  
Sbjct: 39  KHVMSTMNVQKGSKALDVCCGTADWTIALSEAVGSKGQVTGLDFSENML----EVGKQKT 98

Query: 221 ALLNSNLALVRADISRLPFSSGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFV 277
           A L  N+ LV  D   LPF   S D V  G  L   P   +A+ E+ R+++ GG+ V
Sbjct: 99  ASL-ENIQLVHGDAMNLPFDDNSFDYVTIGFGLRNVPDYLSALKEMHRVLKPGGMVV 150

BLAST of Tan0021194 vs. ExPASy Swiss-Prot
Match: O14321 (Sterol 24-C-methyltransferase erg6 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=erg6 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 2.0e-05
Identity = 42/151 (27.81%), Postives = 74/151 (49.01%), Query Frame = 0

Query: 135 LVSFLYERGWRQNFNRSGFPGLDEEFKMAM---EYFKSVEGGL-----LVDASCGSGLFS 194
           L + LYE GW Q+F+ S F    E F  ++   E++ +   G+     ++D  CG G  +
Sbjct: 81  LATDLYEYGWSQSFHFSRF-YKGEAFAQSIARHEHYLAYRMGIKPGSRVLDVGCGVGGPA 140

Query: 195 RKFAKSGSFSG--VIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSSGSVD 254
           R+  +   F+G  ++ L+ ++  + +C ++  K    L+     V+ D   +PF   + D
Sbjct: 141 REITE---FTGCNLVGLNNNDYQISRCNNYAVK--RNLDKKQVFVKGDFMHMPFEDNTFD 200

Query: 255 AVHAGAALHCWPSPSNAIAEITRIMRSGGVF 276
            V+A  A    PS      EI R+++ GGVF
Sbjct: 201 YVYAIEATVHAPSLEGVYGEIFRVLKPGGVF 225

BLAST of Tan0021194 vs. NCBI nr
Match: XP_038889838.1 (uncharacterized methyltransferase At2g41040, chloroplastic [Benincasa hispida])

HSP 1 Score: 656.0 bits (1691), Expect = 1.8e-184
Identity = 323/338 (95.56%), Postives = 328/338 (97.04%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MAVA FFP QTSYNNRH+LSRD RFRSPLR STRR   STVRASS LVLESDLGIQQDQN
Sbjct: 1   MAVATFFPSQTSYNNRHFLSRDIRFRSPLRFSTRRSFFSTVRASSGLVLESDLGIQQDQN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
           SKIDVFSCPVC+EPLLRKGPPG NLPAIYRSGFKCRRCNK+YTSKNIFLDLTVTSGMKEY
Sbjct: 61  SKIDVFSCPVCYEPLLRKGPPGLNLPAIYRSGFKCRRCNKTYTSKNIFLDLTVTSGMKEY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKD+ LLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDSTLLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRERAFQ 300
           GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRERAFQ
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRERAFQ 300

Query: 301 NFGYLTEEEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 339
           NFGYLTEEEIEDLCKSCGLINYSSK+QQSFIMFSAQKP
Sbjct: 301 NFGYLTEEEIEDLCKSCGLINYSSKMQQSFIMFSAQKP 338

BLAST of Tan0021194 vs. NCBI nr
Match: XP_023536953.1 (uncharacterized methyltransferase At2g41040, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 642.1 bits (1655), Expect = 2.7e-180
Identity = 316/338 (93.49%), Postives = 325/338 (96.15%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MAVA FFP QTS NNRH+LSRD RFRSPLR+STRR  S TVRASSTLVLESDL IQQD+N
Sbjct: 1   MAVATFFPYQTSINNRHFLSRDTRFRSPLRVSTRRSFSFTVRASSTLVLESDLSIQQDRN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
           +KIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKC RCNKSYTSKNIFLDLTVTSGMKEY
Sbjct: 61  AKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCGRCNKSYTSKNIFLDLTVTSGMKEY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPG DEEFKMAMEYFKS EGGLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSAEGGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKSG+FSGVIALDFSENMLLQCYDFIKKDA LLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSGAFSGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRERAFQ 300
           GSVDAVHAGAALHCWPSPSNAIAEITR+MRSGGVFVGTTFLRYTSSTPW++R SRERAFQ
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRVMRSGGVFVGTTFLRYTSSTPWFVRISRERAFQ 300

Query: 301 NFGYLTEEEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 339
           NFGYLTE+EIEDLCKSCGLINYSSKIQQSFIMFSAQKP
Sbjct: 301 NFGYLTEKEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 338

BLAST of Tan0021194 vs. NCBI nr
Match: XP_022965827.1 (uncharacterized methyltransferase At2g41040, chloroplastic [Cucurbita maxima])

HSP 1 Score: 641.0 bits (1652), Expect = 5.9e-180
Identity = 316/338 (93.49%), Postives = 323/338 (95.56%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MA A FFP QTS+NNRHYLSRD RFRSPLR+STRR  S TVRASSTLVLESDL IQQD+N
Sbjct: 1   MAAATFFPYQTSFNNRHYLSRDTRFRSPLRVSTRRSFSFTVRASSTLVLESDLSIQQDRN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
           SKIDVFSCPVCFEPLLR GPPGFNLPAIYRSGFKC RCNKSYTSKNIFLDLTVTSGMKEY
Sbjct: 61  SKIDVFSCPVCFEPLLRTGPPGFNLPAIYRSGFKCGRCNKSYTSKNIFLDLTVTSGMKEY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPG DEEFKMAMEYFKS EGGLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSAEGGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKSG+FSGVIALDFSENMLLQCYDFIKKDA LLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSGAFSGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRERAFQ 300
           GSVDAVHAGAALHCWPSPSNAIAEITR MRSGGVFVGTTFLRYTSSTPW++R SRERAFQ
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRAMRSGGVFVGTTFLRYTSSTPWFVRISRERAFQ 300

Query: 301 NFGYLTEEEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 339
           NFGYLTE+EIEDLCKSCGLINYSSKIQQSFIMFSAQKP
Sbjct: 301 NFGYLTEKEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 338

BLAST of Tan0021194 vs. NCBI nr
Match: XP_022142970.1 (uncharacterized methyltransferase At2g41040, chloroplastic [Momordica charantia])

HSP 1 Score: 637.1 bits (1642), Expect = 8.6e-179
Identity = 314/338 (92.90%), Postives = 324/338 (95.86%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MAVA  FP QTSYNNR YLSRD RFRSPLRLSTRR   S +RASSTLVLESDLGIQQDQN
Sbjct: 1   MAVATLFPSQTSYNNRRYLSRDSRFRSPLRLSTRRSF-SFIRASSTLVLESDLGIQQDQN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
           SK+D+FSCPVC+EPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVT+GM++Y
Sbjct: 61  SKVDLFSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTAGMRDY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPG DEEFKMAMEYFKS EGGLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSAEGGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKSGSFSGV+ALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSGSFSGVVALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRERAFQ 300
           GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSR+R  Q
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRQRFSQ 300

Query: 301 NFGYLTEEEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 339
            FGYLTEEEIEDLCKSCGLINYSSK+QQSFIMFSAQKP
Sbjct: 301 EFGYLTEEEIEDLCKSCGLINYSSKVQQSFIMFSAQKP 337

BLAST of Tan0021194 vs. NCBI nr
Match: XP_004139262.1 (uncharacterized methyltransferase At2g41040, chloroplastic [Cucumis sativus])

HSP 1 Score: 636.7 bits (1641), Expect = 1.1e-178
Identity = 312/338 (92.31%), Postives = 323/338 (95.56%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MAVA FFP QTSY NRHYLSRD +FRSPLRLST R + ST+RASS LVLESD GIQQDQN
Sbjct: 1   MAVATFFPSQTSYKNRHYLSRDIQFRSPLRLSTPRSILSTIRASSALVLESDSGIQQDQN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
            KIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY
Sbjct: 61  LKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVS+LYERGWRQNFN+SGFPGLDEEFKMAMEYFKSVEGGLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSYLYERGWRQNFNQSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKSGSF+GVIALDFSENMLLQCYDFIKKDA LLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSGSFAGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRERAFQ 300
           GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSST WY+RF RER FQ
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTLWYLRFLRERGFQ 300

Query: 301 NFGYLTEEEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 339
            +GYLTEEEIE+LCKSCGLINYSSK+Q+SFIMFSAQKP
Sbjct: 301 PYGYLTEEEIEELCKSCGLINYSSKVQRSFIMFSAQKP 338

BLAST of Tan0021194 vs. ExPASy TrEMBL
Match: A0A6J1HLD0 (uncharacterized methyltransferase At2g41040, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111465594 PE=4 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 2.9e-180
Identity = 316/338 (93.49%), Postives = 323/338 (95.56%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MA A FFP QTS+NNRHYLSRD RFRSPLR+STRR  S TVRASSTLVLESDL IQQD+N
Sbjct: 1   MAAATFFPYQTSFNNRHYLSRDTRFRSPLRVSTRRSFSFTVRASSTLVLESDLSIQQDRN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
           SKIDVFSCPVCFEPLLR GPPGFNLPAIYRSGFKC RCNKSYTSKNIFLDLTVTSGMKEY
Sbjct: 61  SKIDVFSCPVCFEPLLRTGPPGFNLPAIYRSGFKCGRCNKSYTSKNIFLDLTVTSGMKEY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPG DEEFKMAMEYFKS EGGLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSAEGGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKSG+FSGVIALDFSENMLLQCYDFIKKDA LLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSGAFSGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRERAFQ 300
           GSVDAVHAGAALHCWPSPSNAIAEITR MRSGGVFVGTTFLRYTSSTPW++R SRERAFQ
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRAMRSGGVFVGTTFLRYTSSTPWFVRISRERAFQ 300

Query: 301 NFGYLTEEEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 339
           NFGYLTE+EIEDLCKSCGLINYSSKIQQSFIMFSAQKP
Sbjct: 301 NFGYLTEKEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 338

BLAST of Tan0021194 vs. ExPASy TrEMBL
Match: A0A6J1CMF3 (uncharacterized methyltransferase At2g41040, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111012967 PE=4 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 4.1e-179
Identity = 314/338 (92.90%), Postives = 324/338 (95.86%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MAVA  FP QTSYNNR YLSRD RFRSPLRLSTRR   S +RASSTLVLESDLGIQQDQN
Sbjct: 1   MAVATLFPSQTSYNNRRYLSRDSRFRSPLRLSTRRSF-SFIRASSTLVLESDLGIQQDQN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
           SK+D+FSCPVC+EPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVT+GM++Y
Sbjct: 61  SKVDLFSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTAGMRDY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPG DEEFKMAMEYFKS EGGLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSAEGGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKSGSFSGV+ALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSGSFSGVVALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRERAFQ 300
           GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSR+R  Q
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRQRFSQ 300

Query: 301 NFGYLTEEEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 339
            FGYLTEEEIEDLCKSCGLINYSSK+QQSFIMFSAQKP
Sbjct: 301 EFGYLTEEEIEDLCKSCGLINYSSKVQQSFIMFSAQKP 337

BLAST of Tan0021194 vs. ExPASy TrEMBL
Match: A0A1S3C4D9 (uncharacterized methyltransferase At2g41040, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496525 PE=4 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 7.1e-179
Identity = 312/338 (92.31%), Postives = 322/338 (95.27%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MAVA FFP QTSYNN HYLSR+ +FR PLRLST R   ST+RASS LVLESD G QQDQN
Sbjct: 1   MAVATFFPSQTSYNNSHYLSRNIQFRYPLRLSTPRSFLSTIRASSALVLESDSGTQQDQN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
           SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY
Sbjct: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVS+LYERGWRQNFN+SGFPGLDEEFKMAMEYFKSVEGGLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSYLYERGWRQNFNQSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDA LLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRERAFQ 300
           GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWY+RF RER FQ
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYLRFLRERGFQ 300

Query: 301 NFGYLTEEEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 339
            +GYLTEEEIE+LCKSCGLINYSSK+Q+SFIMFSAQKP
Sbjct: 301 PYGYLTEEEIEELCKSCGLINYSSKVQRSFIMFSAQKP 338

BLAST of Tan0021194 vs. ExPASy TrEMBL
Match: A0A0A0LG50 (Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G009410 PE=4 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 1.3e-177
Identity = 312/339 (92.04%), Postives = 323/339 (95.28%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MAVA FFP QTSY NRHYLSRD +FRSPLRLST R + ST+RASS LVLESD GIQQDQN
Sbjct: 1   MAVATFFPSQTSYKNRHYLSRDIQFRSPLRLSTPRSILSTIRASSALVLESDSGIQQDQN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
            KIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY
Sbjct: 61  LKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVS+LYERGWRQNFN+SGFPGLDEEFKMAMEYFKSVEGGLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSYLYERGWRQNFNQSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKSGSF+GVIALDFSENMLLQCYDFIKKDA LLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSGSFAGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRE-RAF 300
           GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSST WY+RF RE R F
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTLWYLRFLREQRGF 300

Query: 301 QNFGYLTEEEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 339
           Q +GYLTEEEIE+LCKSCGLINYSSK+Q+SFIMFSAQKP
Sbjct: 301 QPYGYLTEEEIEELCKSCGLINYSSKVQRSFIMFSAQKP 339

BLAST of Tan0021194 vs. ExPASy TrEMBL
Match: A0A6J1FCL5 (uncharacterized methyltransferase At2g41040, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111444178 PE=4 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 1.3e-177
Identity = 312/338 (92.31%), Postives = 322/338 (95.27%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MAVA FFP QT+ NNRH+LS D RFRSPLR+S+RR  S TVRASSTLVLESDL IQQD+N
Sbjct: 1   MAVATFFPCQTAINNRHFLSLDTRFRSPLRVSSRRSFSFTVRASSTLVLESDLSIQQDRN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
           SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKC RCNKSYTSKNIFLDLTVTSGMKEY
Sbjct: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCGRCNKSYTSKNIFLDLTVTSGMKEY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPG DEEFKMAMEYFKS E GLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSAEDGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKS +FSGVIALDFSENMLLQCYDFIKKDA LLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSEAFSGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRERAFQ 300
           GSVDAVHAGAALHCWPSPSNAIAEITR+MRSGGVFVGTTFLRYTSSTPW++R SRERAFQ
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRVMRSGGVFVGTTFLRYTSSTPWFVRISRERAFQ 300

Query: 301 NFGYLTEEEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 339
           NFGYLTE+EIEDLCKSCGLINYSSKIQQSFIMFSAQKP
Sbjct: 301 NFGYLTEKEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 338

BLAST of Tan0021194 vs. TAIR 10
Match: AT2G41040.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 419.9 bits (1078), Expect = 2.0e-117
Identity = 212/332 (63.86%), Postives = 255/332 (76.81%), Query Frame = 0

Query: 14  NNRHYLSRDFRFRSP-LRLSTRRCVSSTVRASSTLVLESDLGIQQDQNSKID-----VFS 73
           N   + S D   +SP LR S    V S   +++   +     I +++  KI+     VF+
Sbjct: 23  NRSSFFSSD--SQSPLLRFSASTSVRSRFPSAAISAVAPKSDINKNETPKIEIEETQVFA 82

Query: 74  CPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEYVEVKPGG 133
           CPVC+EPL+RKGP G NL AIYRSGFKC +CNK+Y+SK+ +LDLTVT+ + +Y EVKP  
Sbjct: 83  CPVCYEPLMRKGPSGINLQAIYRSGFKCGQCNKTYSSKDEYLDLTVTADLDDYNEVKPIT 142

Query: 134 TELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASCGSGLFSR 193
           TELFRSPLVSFLYERGWRQ F RSGFPG DEEF+MA EYFK  EGGLLVD SCGSGLFSR
Sbjct: 143 TELFRSPLVSFLYERGWRQAFKRSGFPGPDEEFRMAEEYFKEAEGGLLVDVSCGSGLFSR 202

Query: 194 KFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNS-NLALVRADISRLPFSSGSVDAV 253
           KFA+SG +SGVIALD+SENML QC +FIK D    NS N+A+VRAD+SRLPF SGSVDAV
Sbjct: 203 KFAQSGKYSGVIALDYSENMLRQCKEFIKNDNTFDNSTNIAVVRADVSRLPFPSGSVDAV 262

Query: 254 HAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRERAFQNFGYLT 313
           HAGAALHCWPSP+NAIAEI R++RSGGVFVGTTFLRY+ STPW IR  + R  Q++ YL 
Sbjct: 263 HAGAALHCWPSPTNAIAEICRVLRSGGVFVGTTFLRYSPSTPWIIRPFQSRILQSYNYLM 322

Query: 314 EEEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 339
           ++EI+D+C SCGL +Y   IQ SFIMF+A+KP
Sbjct: 323 QDEIKDVCTSCGLTDYEDYIQDSFIMFTARKP 352

BLAST of Tan0021194 vs. TAIR 10
Match: AT1G78140.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 288.5 bits (737), Expect = 7.0e-78
Identity = 157/323 (48.61%), Postives = 203/323 (62.85%), Query Frame = 0

Query: 37  VSSTVRASSTLVLESDLGIQQDQNSKID--------------VFSCPVCFEPLL-RKGPP 96
           VS +  ASS     + + ++ + NS +D              + +CP+C+  L     P 
Sbjct: 38  VSRSAHASS-----ASVSVETNSNSNVDFVIEKKDKNRGEKKILACPICYNSLAWISQPN 97

Query: 97  GFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEYVEVKPGGTELFRSPLVSFLYE 156
           G    A      +C  C +SY+     LDL V SG K Y E  P  TELFR+PLVSFLYE
Sbjct: 98  GLIESAASGIQVQCNTCKRSYSGNETHLDLAVASGSKRYSEPMPLSTELFRTPLVSFLYE 157

Query: 157 RGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASCGSGLFSRKFAKSGSFSGVIAL 216
           RGWRQNF   GFPG ++EF+MA  Y K V GG ++DASCGSG+FSR F +S  FS VIAL
Sbjct: 158 RGWRQNFIWGGFPGPEKEFEMAKAYLKPVLGGNIIDASCGSGMFSRLFTRSDLFSLVIAL 217

Query: 217 DFSENMLLQCYDFIKKDAALLN-SNLALVRADISRLPFSSGSVDAVHAGAALHCWPSPSN 276
           D+SENML QCY+ + K+    N   L LVRADI+RLPF SGSVDAVHAGAALHCWPSPS+
Sbjct: 218 DYSENMLRQCYELLNKEENFPNKEKLVLVRADIARLPFLSGSVDAVHAGAALHCWPSPSS 277

Query: 277 AIAEITRIMRSGGVFVGTTFLRYTSSTPW--YIRFSRERAFQNFG---YLTEEEIEDLCK 336
           A+AEI+R++R GGVFV TTF+ Y     +  +++  R+   +  G   +L E E+ED+CK
Sbjct: 278 AVAEISRVLRPGGVFVATTFI-YDGPFSFIPFLKNLRQEIMRYSGSHIFLNERELEDICK 337

Query: 337 SCGLINYSSKIQQSFIMFSAQKP 339
           +CGL+N++      FIM SA KP
Sbjct: 338 ACGLVNFTRVRNGPFIMLSATKP 354

BLAST of Tan0021194 vs. TAIR 10
Match: AT5G13710.1 (sterol methyltransferase 1 )

HSP 1 Score: 43.1 bits (100), Expect = 5.1e-04
Identity = 42/149 (28.19%), Postives = 70/149 (46.98%), Query Frame = 0

Query: 135 LVSFLYERGWRQNFN---RSGFPGLDEEFKMAMEYFKSVEGGL-----LVDASCGSGLFS 194
           L +  YE GW ++F+   R     L E  K   E+F +++ G+     ++D  CG G   
Sbjct: 51  LATSFYEYGWGESFHFAQRWKGESLRESIK-RHEHFLALQLGIQPGQKVLDVGCGIGGPL 110

Query: 195 RKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSSGSVDAV 254
           R+ A+  S S V  L+ +E  + +  +  +   A ++     V+AD  ++PF   S DAV
Sbjct: 111 REIARF-SNSVVTGLNNNEYQITRGKELNR--LAGVDKTCNFVKADFMKMPFPENSFDAV 170

Query: 255 HAGAALHCWPSPSNAIAEITRIMRSGGVF 276
           +A  A    P       EI R+++ G  F
Sbjct: 171 YAIEATCHAPDAYGCYKEIYRVLKPGQCF 195

BLAST of Tan0021194 vs. TAIR 10
Match: AT5G13710.2 (sterol methyltransferase 1 )

HSP 1 Score: 43.1 bits (100), Expect = 5.1e-04
Identity = 42/149 (28.19%), Postives = 70/149 (46.98%), Query Frame = 0

Query: 135 LVSFLYERGWRQNFN---RSGFPGLDEEFKMAMEYFKSVEGGL-----LVDASCGSGLFS 194
           L +  YE GW ++F+   R     L E  K   E+F +++ G+     ++D  CG G   
Sbjct: 51  LATSFYEYGWGESFHFAQRWKGESLRESIK-RHEHFLALQLGIQPGQKVLDVGCGIGGPL 110

Query: 195 RKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSSGSVDAV 254
           R+ A+  S S V  L+ +E  + +  +  +   A ++     V+AD  ++PF   S DAV
Sbjct: 111 REIARF-SNSVVTGLNNNEYQITRGKELNR--LAGVDKTCNFVKADFMKMPFPENSFDAV 170

Query: 255 HAGAALHCWPSPSNAIAEITRIMRSGGVF 276
           +A  A    P       EI R+++ G  F
Sbjct: 171 YAIEATCHAPDAYGCYKEIYRVLKPGQCF 195

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0WPT72.8e-11663.86Uncharacterized methyltransferase At2g41040, chloroplastic OS=Arabidopsis thalia... [more]
Q8LBV49.8e-7748.61Uncharacterized methyltransferase At1g78140, chloroplastic OS=Arabidopsis thalia... [more]
Q5HP749.0e-0633.33Demethylmenaquinone methyltransferase OS=Staphylococcus epidermidis (strain ATCC... [more]
Q8CSH99.0e-0633.33Demethylmenaquinone methyltransferase OS=Staphylococcus epidermidis (strain ATCC... [more]
O143212.0e-0527.81Sterol 24-C-methyltransferase erg6 OS=Schizosaccharomyces pombe (strain 972 / AT... [more]
Match NameE-valueIdentityDescription
XP_038889838.11.8e-18495.56uncharacterized methyltransferase At2g41040, chloroplastic [Benincasa hispida][more]
XP_023536953.12.7e-18093.49uncharacterized methyltransferase At2g41040, chloroplastic [Cucurbita pepo subsp... [more]
XP_022965827.15.9e-18093.49uncharacterized methyltransferase At2g41040, chloroplastic [Cucurbita maxima][more]
XP_022142970.18.6e-17992.90uncharacterized methyltransferase At2g41040, chloroplastic [Momordica charantia][more]
XP_004139262.11.1e-17892.31uncharacterized methyltransferase At2g41040, chloroplastic [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A6J1HLD02.9e-18093.49uncharacterized methyltransferase At2g41040, chloroplastic OS=Cucurbita maxima O... [more]
A0A6J1CMF34.1e-17992.90uncharacterized methyltransferase At2g41040, chloroplastic OS=Momordica charanti... [more]
A0A1S3C4D97.1e-17992.31uncharacterized methyltransferase At2g41040, chloroplastic isoform X2 OS=Cucumis... [more]
A0A0A0LG501.3e-17792.04Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G00... [more]
A0A6J1FCL51.3e-17792.31uncharacterized methyltransferase At2g41040, chloroplastic OS=Cucurbita moschata... [more]
Match NameE-valueIdentityDescription
AT2G41040.12.0e-11763.86S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G78140.17.0e-7848.61S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G13710.15.1e-0428.19sterol methyltransferase 1 [more]
AT5G13710.25.1e-0428.19sterol methyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013216Methyltransferase type 11PFAMPF08241Methyltransf_11coord: 177..276
e-value: 8.3E-18
score: 64.8
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 131..299
e-value: 6.7E-28
score: 99.6
NoneNo IPR availablePANTHERPTHR43591:SF66SUBFAMILY NOT NAMEDcoord: 24..292
NoneNo IPR availablePANTHERPTHR43591METHYLTRANSFERASEcoord: 24..292
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 175..281
e-value: 1.6621E-11
score: 58.2102
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 139..291

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0021194.1Tan0021194.1mRNA
Tan0021194.2Tan0021194.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0006744 ubiquinone biosynthetic process
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0050661 NADP binding
molecular_function GO:0004499 N,N-dimethylaniline monooxygenase activity