Tan0021194.2 (mRNA) Snake gourd v1

Overview
NameTan0021194.2
TypemRNA
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionMethyltransf_11 domain-containing protein
LocationLG08: 3158207 .. 3162510 (+)
Sequence length2755
RNA-Seq ExpressionTan0021194.2
SyntenyTan0021194.2
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGTCATTAAAACCAGCTCCTTAATCTTGCTGCTGATTCCTGAAAGGGGAAGAAGAACCAGAGGTCCGAGAAGCTTCCAATCCATTTTCATGGCGGTAGCTATTTTCTTTCCTCCTCAAACTTCATACAACAATCGCCATTATCTTTCTCGCGATTTCCGATTTCGGTCTCCTCTTCGCTTGTCCACTCGTCGTTGTGTCTCCTCTACCGTTCGGGCGAGCTCGACGCTCGTTCTTGAGTCGGTAAGCTCCACCTTAACTTTCTTGTTCCAATTTTCGTTTATAATTCGAATTTTTGATGTTATTGCTGTTAAAACGAATCTCTGTTGTGCGTTGAAGTTGAATTGTATTGTGATATGGAGTTGACGATTCGGACATGTATGTTTCGGGATTGACAATAGGCTGAATTTTGCAATAATTTGGAGAGGAGTATGTAGATGCTACCCCGTGTTTGGCTTTTTAGGGAAATTATTGTTTGAGGAATTTGTAATTTCAACTCAACTTCGAACTGTTTCATTTAGTCGAATTTGGAGTTTCTATTATTATTATTATTTGTTTCCCTCAGGATTTAGGCATTCAACAGGATCAGAATTCAAAAATTGACGTGTTTTCATGCCCTGTTTGTTTTGAGCCACTGTTGAGAAAAGGCCCGCCAGGTTTTAACTTGTAAGTTGACTTAATGTGATGTATTCCATCTTCTGATATTTAACTTGTTGCATTTTATGATATTTCGACCCGAGTGAAGTGGAAAATTTGCAGGCCAGCTATTTATAGGTCTGGTTTCAAGTGTAGAAGATGCAATAAGTCATACACTAGCAAAAACATCTTTTTGGATCTCACAGTTACTTCTGGAATGAAGGAATATGTTGAAGTCAAACCTGGGGGAACTGAGCTGTTTCGGTATCTTTCTGATATTTTCTTTCATTTTTTCTTCTAAATGTTTCATTCACTTCTAAGTTTTCTGATTTTGCTTGCTTCATCTTAAGTGTTAAGTTGATGTCACCCATTACAACTTGTCTAACGAAAGGAAGTGGTACTGTGTTATTGCCATTTTTTCCCCTGAAGGAGACAATTCTCTATTGCTTATGGTTTTTGTACTTTGTAGGAGTCCACTCGTTTCATTTCTGTATGAAAGAGGATGGCGACAAAACTTTAACCGAAGTGGTTTCCCCGGCCTCGATGAAGAAGTATGCTTCCTCTCAATCATAACACTACCATTCAAACGTACGAATATTTTATATTGATATTTTGATGCCGTTCAAAAGATTACTCGTGGAGAAAAACAAGTGTTATCGTATTGTTCATGAGTTGCCTTGGCATTGGATATATGGCCTTTTCATTTCCTTGAATAATGTGAATATGGGCTGATATTTTTCTTTAAATATATGCCATCGCAGTTTAAAATGGCCATGGAGTATTTTAAATCTGTTGAAGGTGGTCTTTTAGTGGATGCAAGCTGTGGTAGTGGTTTGTTCTCCAGAAAATTTGCAAAATCTGGGTCTTTTTCAGGTGTTATTGCACTTGATTTTTCTGAGAATATGCTCCTCCAATGTTATGATTTCATAAAGAAAGATGCTGCGCTTTTGAACAGGTGATTTTATTTTATGACTTTTATACTCTTGTTTAATTAATTCCTTAGAACACAACTGATATACCCTGCTAGCTTGTGTATTAAGTCCTCTCAACTTGCAAGAAAAGTGAAGGCTATCCTAATTGGTATATCTTCTTTTGGAAAATATGCAGTAATCTTGCTCTTGTGAGGGCAGATATCTCTAGGCTTCCCTTTTCATCAGGATCAGTGGATGCTGTTCATGCTGGTGCAGCATTGCATTGCTGGCCATCTCCTTCCAATGCTGTAAGCATTTTGTCTTGTAGGATCATATTATATCATTTGATTTCCTACCATTAGTTGTAATCCGCTGAATGCAACCAGGTTATAGAATATCACTGTCAACTTCATCCCATTATTTTCTTGATCAAAAAAAAAAACTTCATCCCATTATTTTCACTATAGAGTTTCTCTTCTCAACCAGTGTATGTGATTGAATTCATTTAAAGTTTCTCTTTCCATGCTCGCTACAATTCCTCGTGGTACTTAGTCCATTGAAAGGAAGCGATCAAAGCTTTCCCTTCATAATAGTTATATGCACTCAATTCACAAAATAATAATATGTTATTGTGTCTGAAACAACGGCAGGTTCCACTGGGCAATTGGTTTTCTGATATATCTTCTTGACTAATGCATTTCTTTTAGGAAGTTCTATTTGAAATCTTTAGGAATTAGCTCCTGAGTATTTTTGTATGTTTTTAGCAACGAGGCATCCATTTATGATATTTTCAGCTCAGATTCTCATTGTTCAGATGATAAGGTCAATATTTTTCAACTTCTTTAGTAATGGTTTCTTTTAACTCTTCTTATCAGATTGCTGAGATCACTCGTATAATGCGGAGTGGTGGAGTATTTGTAGGAACTACTTTTTTGCGGTACACTTCATCCACTCCCTGGTATATAAGATTTTCTAGAGAGGTATACCCTTAATCTATTCTCTCTCATTTTTGCACTTCTAGATATCACTAGTCAGAAGCAGTTATGACAAACATCTGTGTTATCTGATTGATATTTCTGTTCCAGAGTCATGAATTTGGTAATCATGCATTTATATACACGAAATCCGTCCTTGCATATTTTCCTTTCCAATTAACTATGGTTAATTTCTTGATTCTGTTACTTCAAAATGACAAAAAATGTATTTGGGCACCCTGTAAGGTGGTATAAACAAACTAGCTTCCTTCATGGTTAATTGAACTTCTTGCTGGCTTATATTTTGTCATTTTCATTGGACTGAGTATTGGTATTTATTAGAGTCCATAAATCTTCTGGAACCATACATAAACATGAACTAACATAGTTTGTTTTACTTTCTCAAGTTTATAGAAGATCTCCATTTATCAGTTCGGACAACTTTTTCTCATGTAAAGATTGATATCTGTGTGGAATGTGTTCAAAACTCAGTTTCTTTTGTGCTTGCGCCACCATTTTTTTGGGTTGCTTTTGGATGAGAGGCCAACTTTTATGGAGATAAATGAAAGGATACAGAGGAGGCCGAAAAAGCCATGCCAAAAGCGGAGATTAAATAACTCACAATAAACTTACAAAAAGAACTCATCTAGTAAAGATAACTATGAAAAAAATTTAAACTAAAGTACTAAAATGTGTCAAATCCCAAACACATCATCCCACGACCTACATAATCCAACCAACATTCCCCTATTTCTTTCTAGTCAAACAAACTACAGTATGATGAAGAAGCTAACTTGTTGGAAAGCACAACCTTCTAGGAAAAGGAGGATCCAAGACGACGCCCTCCATCATCCTGTAGATACCGACCTCTAAACTACAAATAATCGATACCAACTGATCCAAAATAAACATCTAAATTGCTACTTACCATATTATGATAAATAATTGTATTGAGGCGACGAGTGAATCTGTGATAAGTATTAGATAACCTGAGGTTCCATCTCAAAACCAATTGGTAATGAGAAGAGTAGCTCATGTATCTTATAAATTGTGAGGTCACCTTACATTTTTTCAATGTAGTATCCTCAACATGCCCTCTCAAGATTGTGCCTGTTTGGATTCACCATTAAGTTGGAATTTTGATCGAATACCCGTTTGGGTTTTATGGGCTATGATACCATATTAGATAACAGGAAGTTCCATCTCAAAACCAATTGAGAATGGGAAGAATAACCATGTATATTATAAATCGTGAGATCATTCTACATTTTTCTAGTGAGAGATCCTCAACAATAAGTTCTCCTCTTTGAACAAACTGCATTAATGTTTTGTATATGGAGATGACACTCACGAGATTCAAGGTTCCTCTAATTCTGAAGGATGCAATGATTTTCACATTTTTTCTATATTATCAAGATGGCATTTGAGGCTATAAGTTTTGTGTGCTGCAGCGTGCTTTTCAGAATTTTGGCTACTTAACAGAGGAAGAAATAGAGGATTTGTGCAAATCCTGCGGTCTGATAAACTATTCAAGCAAAATTCAGCAATCTTTCATTATGTTCTCTGCTCAGAAGCCTTGAAAGTAAGTTCTCAGTTTCATTCTCATTTTCATTGTGATGGGTATGTTCATAATCAAACTCTATAGAATTATATTTGATAATTGAATACCATACCATGTAAATTGTTGAAACTGAAAAGTATAATTAATTGAATTGAATAGAATTATTTGTTAATATACTCCAACTGTTTCAAGATTGAGAAGAAACGTGTAAATTGTAATAATGC

mRNA sequence

CGTCATTAAAACCAGCTCCTTAATCTTGCTGCTGATTCCTGAAAGGGGAAGAAGAACCAGAGGTCCGAGAAGCTTCCAATCCATTTTCATGGCGGTAGCTATTTTCTTTCCTCCTCAAACTTCATACAACAATCGCCATTATCTTTCTCGCGATTTCCGATTTCGGTCTCCTCTTCGCTTGTCCACTCGTCGTTGTGTCTCCTCTACCGTTCGGGCGAGCTCGACGCTCGTTCTTGAGTCGGATTTAGGCATTCAACAGGATCAGAATTCAAAAATTGACGTGTTTTCATGCCCTGTTTGTTTTGAGCCACTGTTGAGAAAAGGCCCGCCAGGTTTTAACTTGCCAGCTATTTATAGGTCTGGTTTCAAGTGTAGAAGATGCAATAAGTCATACACTAGCAAAAACATCTTTTTGGATCTCACAGTTACTTCTGGAATGAAGGAATATGTTGAAGTCAAACCTGGGGGAACTGAGCTGTTTCGGAGTCCACTCGTTTCATTTCTGTATGAAAGAGGATGGCGACAAAACTTTAACCGAAGTGGTTTCCCCGGCCTCGATGAAGAATTTAAAATGGCCATGGAGTATTTTAAATCTGTTGAAGGTGGTCTTTTAGTGGATGCAAGCTGTGGTAGTGGTTTGTTCTCCAGAAAATTTGCAAAATCTGGGTCTTTTTCAGGTGTTATTGCACTTGATTTTTCTGAGAATATGCTCCTCCAATGTTATGATTTCATAAAGAAAGATGCTGCGCTTTTGAACAGTAATCTTGCTCTTGTGAGGGCAGATATCTCTAGGCTTCCCTTTTCATCAGGATCAGTGGATGCTGTTCATGCTGGTGCAGCATTGCATTGCTGGCCATCTCCTTCCAATGCTATTGCTGAGATCACTCGTATAATGCGGAGTGGTGGAGTATTTGTAGGAACTACTTTTTTGCGGTACACTTCATCCACTCCCTGGTATATAAGATTTTCTAGAGAGGTATACCCTTAATCTATTCTCTCTCATTTTTGCACTTCTAGATATCACTAGTCAGAAGCAGTTATGACAAACATCTGTGTTATCTGATTGATATTTCTGTTCCAGAGTCATGAATTTGGTAATCATGCATTTATATACACGAAATCCGTCCTTGCATATTTTCCTTTCCAATTAACTATGGTTAATTTCTTGATTCTGTTACTTCAAAATGACAAAAAATGTATTTGGGCACCCTGTAAGGTGGTATAAACAAACTAGCTTCCTTCATGGTTAATTGAACTTCTTGCTGGCTTATATTTTGTCATTTTCATTGGACTGAGTATTGGTATTTATTAGAGTCCATAAATCTTCTGGAACCATACATAAACATGAACTAACATAGTTTGTTTTACTTTCTCAAGTTTATAGAAGATCTCCATTTATCAGTTCGGACAACTTTTTCTCATGTAAAGATTGATATCTGTGTGGAATGTGTTCAAAACTCAGTTTCTTTTGTGCTTGCGCCACCATTTTTTTGGGTTGCTTTTGGATGAGAGGCCAACTTTTATGGAGATAAATGAAAGGATACAGAGGAGGCCGAAAAAGCCATGCCAAAAGCGGAGATTAAATAACTCACAATAAACTTACAAAAAGAACTCATCTAGTAAAGATAACTATGAAAAAAATTTAAACTAAAGTACTAAAATGTGTCAAATCCCAAACACATCATCCCACGACCTACATAATCCAACCAACATTCCCCTATTTCTTTCTAGTCAAACAAACTACAGTATGATGAAGAAGCTAACTTGTTGGAAAGCACAACCTTCTAGGAAAAGGAGGATCCAAGACGACGCCCTCCATCATCCTGTAGATACCGACCTCTAAACTACAAATAATCGATACCAACTGATCCAAAATAAACATCTAAATTGCTACTTACCATATTATGATAAATAATTGTATTGAGGCGACGAGTGAATCTGTGATAAGTATTAGATAACCTGAGGTTCCATCTCAAAACCAATTGGTAATGAGAAGAGTAGCTCATGTATCTTATAAATTGTGAGGTCACCTTACATTTTTTCAATGTAGTATCCTCAACATGCCCTCTCAAGATTGTGCCTGTTTGGATTCACCATTAAGTTGGAATTTTGATCGAATACCCGTTTGGGTTTTATGGGCTATGATACCATATTAGATAACAGGAAGTTCCATCTCAAAACCAATTGAGAATGGGAAGAATAACCATGTATATTATAAATCGTGAGATCATTCTACATTTTTCTAGTGAGAGATCCTCAACAATAAGTTCTCCTCTTTGAACAAACTGCATTAATGTTTTGTATATGGAGATGACACTCACGAGATTCAAGGTTCCTCTAATTCTGAAGGATGCAATGATTTTCACATTTTTTCTATATTATCAAGATGGCATTTGAGGCTATAAGTTTTGTGTGCTGCAGCGTGCTTTTCAGAATTTTGGCTACTTAACAGAGGAAGAAATAGAGGATTTGTGCAAATCCTGCGGTCTGATAAACTATTCAAGCAAAATTCAGCAATCTTTCATTATGTTCTCTGCTCAGAAGCCTTGAAAGTAAGTTCTCAGTTTCATTCTCATTTTCATTGTGATGGGTATGTTCATAATCAAACTCTATAGAATTATATTTGATAATTGAATACCATACCATGTAAATTGTTGAAACTGAAAAGTATAATTAATTGAATTGAATAGAATTATTTGTTAATATACTCCAACTGTTTCAAGATTGAGAAGAAACGTGTAAATTGTAATAATGC

Coding sequence (CDS)

ATGGCGGTAGCTATTTTCTTTCCTCCTCAAACTTCATACAACAATCGCCATTATCTTTCTCGCGATTTCCGATTTCGGTCTCCTCTTCGCTTGTCCACTCGTCGTTGTGTCTCCTCTACCGTTCGGGCGAGCTCGACGCTCGTTCTTGAGTCGGATTTAGGCATTCAACAGGATCAGAATTCAAAAATTGACGTGTTTTCATGCCCTGTTTGTTTTGAGCCACTGTTGAGAAAAGGCCCGCCAGGTTTTAACTTGCCAGCTATTTATAGGTCTGGTTTCAAGTGTAGAAGATGCAATAAGTCATACACTAGCAAAAACATCTTTTTGGATCTCACAGTTACTTCTGGAATGAAGGAATATGTTGAAGTCAAACCTGGGGGAACTGAGCTGTTTCGGAGTCCACTCGTTTCATTTCTGTATGAAAGAGGATGGCGACAAAACTTTAACCGAAGTGGTTTCCCCGGCCTCGATGAAGAATTTAAAATGGCCATGGAGTATTTTAAATCTGTTGAAGGTGGTCTTTTAGTGGATGCAAGCTGTGGTAGTGGTTTGTTCTCCAGAAAATTTGCAAAATCTGGGTCTTTTTCAGGTGTTATTGCACTTGATTTTTCTGAGAATATGCTCCTCCAATGTTATGATTTCATAAAGAAAGATGCTGCGCTTTTGAACAGTAATCTTGCTCTTGTGAGGGCAGATATCTCTAGGCTTCCCTTTTCATCAGGATCAGTGGATGCTGTTCATGCTGGTGCAGCATTGCATTGCTGGCCATCTCCTTCCAATGCTATTGCTGAGATCACTCGTATAATGCGGAGTGGTGGAGTATTTGTAGGAACTACTTTTTTGCGGTACACTTCATCCACTCCCTGGTATATAAGATTTTCTAGAGAGGTATACCCTTAA

Protein sequence

MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQNSKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEYVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASCGSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSSGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSREVYP
Homology
BLAST of Tan0021194.2 vs. ExPASy Swiss-Prot
Match: Q0WPT7 (Uncharacterized methyltransferase At2g41040, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g41040 PE=1 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 1.7e-101
Identity = 189/286 (66.08%), Postives = 222/286 (77.62%), Query Frame = 0

Query: 14  NNRHYLSRDFRFRSP-LRLSTRRCVSSTVRASSTLVLESDLGIQQDQNSKID-----VFS 73
           N   + S D   +SP LR S    V S   +++   +     I +++  KI+     VF+
Sbjct: 23  NRSSFFSSD--SQSPLLRFSASTSVRSRFPSAAISAVAPKSDINKNETPKIEIEETQVFA 82

Query: 74  CPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEYVEVKPGG 133
           CPVC+EPL+RKGP G NL AIYRSGFKC +CNK+Y+SK+ +LDLTVT+ + +Y EVKP  
Sbjct: 83  CPVCYEPLMRKGPSGINLQAIYRSGFKCGQCNKTYSSKDEYLDLTVTADLDDYNEVKPIT 142

Query: 134 TELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASCGSGLFSR 193
           TELFRSPLVSFLYERGWRQ F RSGFPG DEEF+MA EYFK  EGGLLVD SCGSGLFSR
Sbjct: 143 TELFRSPLVSFLYERGWRQAFKRSGFPGPDEEFRMAEEYFKEAEGGLLVDVSCGSGLFSR 202

Query: 194 KFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNS-NLALVRADISRLPFSSGSVDAV 253
           KFA+SG +SGVIALD+SENML QC +FIK D    NS N+A+VRAD+SRLPF SGSVDAV
Sbjct: 203 KFAQSGKYSGVIALDYSENMLRQCKEFIKNDNTFDNSTNIAVVRADVSRLPFPSGSVDAV 262

Query: 254 HAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIR 293
           HAGAALHCWPSP+NAIAEI R++RSGGVFVGTTFLRY+ STPW IR
Sbjct: 263 HAGAALHCWPSPTNAIAEICRVLRSGGVFVGTTFLRYSPSTPWIIR 306

BLAST of Tan0021194.2 vs. ExPASy Swiss-Prot
Match: Q8LBV4 (Uncharacterized methyltransferase At1g78140, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g78140 PE=1 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.6e-67
Identity = 136/261 (52.11%), Postives = 169/261 (64.75%), Query Frame = 0

Query: 37  VSSTVRASSTLVLESDLGIQQDQNSKID--------------VFSCPVCFEPLL-RKGPP 96
           VS +  ASS     + + ++ + NS +D              + +CP+C+  L     P 
Sbjct: 38  VSRSAHASS-----ASVSVETNSNSNVDFVIEKKDKNRGEKKILACPICYNSLAWISQPN 97

Query: 97  GFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEYVEVKPGGTELFRSPLVSFLYE 156
           G    A      +C  C +SY+     LDL V SG K Y E  P  TELFR+PLVSFLYE
Sbjct: 98  GLIESAASGIQVQCNTCKRSYSGNETHLDLAVASGSKRYSEPMPLSTELFRTPLVSFLYE 157

Query: 157 RGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASCGSGLFSRKFAKSGSFSGVIAL 216
           RGWRQNF   GFPG ++EF+MA  Y K V GG ++DASCGSG+FSR F +S  FS VIAL
Sbjct: 158 RGWRQNFIWGGFPGPEKEFEMAKAYLKPVLGGNIIDASCGSGMFSRLFTRSDLFSLVIAL 217

Query: 217 DFSENMLLQCYDFIKKDAALLN-SNLALVRADISRLPFSSGSVDAVHAGAALHCWPSPSN 276
           D+SENML QCY+ + K+    N   L LVRADI+RLPF SGSVDAVHAGAALHCWPSPS+
Sbjct: 218 DYSENMLRQCYELLNKEENFPNKEKLVLVRADIARLPFLSGSVDAVHAGAALHCWPSPSS 277

Query: 277 AIAEITRIMRSGGVFVGTTFL 282
           A+AEI+R++R GGVFV TTF+
Sbjct: 278 AVAEISRVLRPGGVFVATTFI 293

BLAST of Tan0021194.2 vs. ExPASy Swiss-Prot
Match: Q5HP74 (Demethylmenaquinone methyltransferase OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) OX=176279 GN=menG PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 8.0e-06
Identity = 39/117 (33.33%), Postives = 57/117 (48.72%), Query Frame = 0

Query: 161 KMAMEYFKSVEGGLLVDASCGSGLFSRKFAKS-GSFSGVIALDFSENMLLQCYDFIKKDA 220
           K  M      +G   +D  CG+  ++   +++ GS   V  LDFSENML    +  K+  
Sbjct: 39  KHVMSTMNVQKGSKALDVCCGTADWTIALSEAVGSKGQVTGLDFSENML----EVGKQKT 98

Query: 221 ALLNSNLALVRADISRLPFSSGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFV 277
           A L  N+ LV  D   LPF   S D V  G  L   P   +A+ E+ R+++ GG+ V
Sbjct: 99  ASL-ENIQLVHGDAMNLPFDDNSFDYVTIGFGLRNVPDYLSALKEMHRVLKPGGMVV 150

BLAST of Tan0021194.2 vs. ExPASy Swiss-Prot
Match: Q8CSH9 (Demethylmenaquinone methyltransferase OS=Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) OX=176280 GN=menG PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 8.0e-06
Identity = 39/117 (33.33%), Postives = 57/117 (48.72%), Query Frame = 0

Query: 161 KMAMEYFKSVEGGLLVDASCGSGLFSRKFAKS-GSFSGVIALDFSENMLLQCYDFIKKDA 220
           K  M      +G   +D  CG+  ++   +++ GS   V  LDFSENML    +  K+  
Sbjct: 39  KHVMSTMNVQKGSKALDVCCGTADWTIALSEAVGSKGQVTGLDFSENML----EVGKQKT 98

Query: 221 ALLNSNLALVRADISRLPFSSGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFV 277
           A L  N+ LV  D   LPF   S D V  G  L   P   +A+ E+ R+++ GG+ V
Sbjct: 99  ASL-ENIQLVHGDAMNLPFDDNSFDYVTIGFGLRNVPDYLSALKEMHRVLKPGGMVV 150

BLAST of Tan0021194.2 vs. ExPASy Swiss-Prot
Match: O14321 (Sterol 24-C-methyltransferase erg6 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=erg6 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 1.8e-05
Identity = 42/151 (27.81%), Postives = 74/151 (49.01%), Query Frame = 0

Query: 135 LVSFLYERGWRQNFNRSGFPGLDEEFKMAM---EYFKSVEGGL-----LVDASCGSGLFS 194
           L + LYE GW Q+F+ S F    E F  ++   E++ +   G+     ++D  CG G  +
Sbjct: 81  LATDLYEYGWSQSFHFSRF-YKGEAFAQSIARHEHYLAYRMGIKPGSRVLDVGCGVGGPA 140

Query: 195 RKFAKSGSFSG--VIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSSGSVD 254
           R+  +   F+G  ++ L+ ++  + +C ++  K    L+     V+ D   +PF   + D
Sbjct: 141 REITE---FTGCNLVGLNNNDYQISRCNNYAVK--RNLDKKQVFVKGDFMHMPFEDNTFD 200

Query: 255 AVHAGAALHCWPSPSNAIAEITRIMRSGGVF 276
            V+A  A    PS      EI R+++ GGVF
Sbjct: 201 YVYAIEATVHAPSLEGVYGEIFRVLKPGGVF 225

BLAST of Tan0021194.2 vs. NCBI nr
Match: XP_038889838.1 (uncharacterized methyltransferase At2g41040, chloroplastic [Benincasa hispida])

HSP 1 Score: 572.8 bits (1475), Expect = 1.8e-159
Identity = 282/296 (95.27%), Postives = 286/296 (96.62%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MAVA FFP QTSYNNRH+LSRD RFRSPLR STRR   STVRASS LVLESDLGIQQDQN
Sbjct: 1   MAVATFFPSQTSYNNRHFLSRDIRFRSPLRFSTRRSFFSTVRASSGLVLESDLGIQQDQN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
           SKIDVFSCPVC+EPLLRKGPPG NLPAIYRSGFKCRRCNK+YTSKNIFLDLTVTSGMKEY
Sbjct: 61  SKIDVFSCPVCYEPLLRKGPPGLNLPAIYRSGFKCRRCNKTYTSKNIFLDLTVTSGMKEY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKD+ LLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDSTLLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRE 297
           GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRE
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRE 296

BLAST of Tan0021194.2 vs. NCBI nr
Match: KGN60753.1 (hypothetical protein Csa_019482 [Cucumis sativus])

HSP 1 Score: 561.6 bits (1446), Expect = 4.0e-156
Identity = 276/296 (93.24%), Postives = 283/296 (95.61%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MAVA FFP QTSY NRHYLSRD +FRSPLRLST R + ST+RASS LVLESD GIQQDQN
Sbjct: 1   MAVATFFPSQTSYKNRHYLSRDIQFRSPLRLSTPRSILSTIRASSALVLESDSGIQQDQN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
            KIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY
Sbjct: 61  LKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVS+LYERGWRQNFN+SGFPGLDEEFKMAMEYFKSVEGGLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSYLYERGWRQNFNQSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKSGSF+GVIALDFSENMLLQCYDFIKKDA LLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSGSFAGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRE 297
           GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSST WY+RF RE
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTLWYLRFLRE 296

BLAST of Tan0021194.2 vs. NCBI nr
Match: XP_004139262.1 (uncharacterized methyltransferase At2g41040, chloroplastic [Cucumis sativus])

HSP 1 Score: 561.6 bits (1446), Expect = 4.0e-156
Identity = 276/296 (93.24%), Postives = 283/296 (95.61%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MAVA FFP QTSY NRHYLSRD +FRSPLRLST R + ST+RASS LVLESD GIQQDQN
Sbjct: 1   MAVATFFPSQTSYKNRHYLSRDIQFRSPLRLSTPRSILSTIRASSALVLESDSGIQQDQN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
            KIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY
Sbjct: 61  LKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVS+LYERGWRQNFN+SGFPGLDEEFKMAMEYFKSVEGGLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSYLYERGWRQNFNQSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKSGSF+GVIALDFSENMLLQCYDFIKKDA LLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSGSFAGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRE 297
           GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSST WY+RF RE
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTLWYLRFLRE 296

BLAST of Tan0021194.2 vs. NCBI nr
Match: XP_008456627.1 (PREDICTED: uncharacterized methyltransferase At2g41040, chloroplastic isoform X1 [Cucumis melo] >KAA0063556.1 putative methyltransferase [Cucumis melo var. makuwa] >TYJ97883.1 putative methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 561.2 bits (1445), Expect = 5.3e-156
Identity = 276/296 (93.24%), Postives = 282/296 (95.27%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MAVA FFP QTSYNN HYLSR+ +FR PLRLST R   ST+RASS LVLESD G QQDQN
Sbjct: 1   MAVATFFPSQTSYNNSHYLSRNIQFRYPLRLSTPRSFLSTIRASSALVLESDSGTQQDQN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
           SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY
Sbjct: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVS+LYERGWRQNFN+SGFPGLDEEFKMAMEYFKSVEGGLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSYLYERGWRQNFNQSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDA LLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRE 297
           GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWY+RF RE
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYLRFLRE 296

BLAST of Tan0021194.2 vs. NCBI nr
Match: XP_008456635.1 (PREDICTED: uncharacterized methyltransferase At2g41040, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 561.2 bits (1445), Expect = 5.3e-156
Identity = 276/296 (93.24%), Postives = 282/296 (95.27%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MAVA FFP QTSYNN HYLSR+ +FR PLRLST R   ST+RASS LVLESD G QQDQN
Sbjct: 1   MAVATFFPSQTSYNNSHYLSRNIQFRYPLRLSTPRSFLSTIRASSALVLESDSGTQQDQN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
           SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY
Sbjct: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVS+LYERGWRQNFN+SGFPGLDEEFKMAMEYFKSVEGGLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSYLYERGWRQNFNQSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDA LLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRE 297
           GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWY+RF RE
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYLRFLRE 296

BLAST of Tan0021194.2 vs. ExPASy TrEMBL
Match: A0A0A0LG50 (Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G009410 PE=4 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 2.0e-156
Identity = 276/296 (93.24%), Postives = 283/296 (95.61%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MAVA FFP QTSY NRHYLSRD +FRSPLRLST R + ST+RASS LVLESD GIQQDQN
Sbjct: 1   MAVATFFPSQTSYKNRHYLSRDIQFRSPLRLSTPRSILSTIRASSALVLESDSGIQQDQN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
            KIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY
Sbjct: 61  LKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVS+LYERGWRQNFN+SGFPGLDEEFKMAMEYFKSVEGGLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSYLYERGWRQNFNQSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKSGSF+GVIALDFSENMLLQCYDFIKKDA LLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSGSFAGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRE 297
           GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSST WY+RF RE
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTLWYLRFLRE 296

BLAST of Tan0021194.2 vs. ExPASy TrEMBL
Match: A0A5A7VAW1 (Putative methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00970 PE=4 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 2.6e-156
Identity = 276/296 (93.24%), Postives = 282/296 (95.27%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MAVA FFP QTSYNN HYLSR+ +FR PLRLST R   ST+RASS LVLESD G QQDQN
Sbjct: 1   MAVATFFPSQTSYNNSHYLSRNIQFRYPLRLSTPRSFLSTIRASSALVLESDSGTQQDQN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
           SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY
Sbjct: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVS+LYERGWRQNFN+SGFPGLDEEFKMAMEYFKSVEGGLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSYLYERGWRQNFNQSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDA LLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRE 297
           GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWY+RF RE
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYLRFLRE 296

BLAST of Tan0021194.2 vs. ExPASy TrEMBL
Match: A0A1S3C4D9 (uncharacterized methyltransferase At2g41040, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496525 PE=4 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 2.6e-156
Identity = 276/296 (93.24%), Postives = 282/296 (95.27%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MAVA FFP QTSYNN HYLSR+ +FR PLRLST R   ST+RASS LVLESD G QQDQN
Sbjct: 1   MAVATFFPSQTSYNNSHYLSRNIQFRYPLRLSTPRSFLSTIRASSALVLESDSGTQQDQN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
           SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY
Sbjct: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVS+LYERGWRQNFN+SGFPGLDEEFKMAMEYFKSVEGGLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSYLYERGWRQNFNQSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDA LLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRE 297
           GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWY+RF RE
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYLRFLRE 296

BLAST of Tan0021194.2 vs. ExPASy TrEMBL
Match: A0A1S3C3A2 (uncharacterized methyltransferase At2g41040, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496525 PE=4 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 2.6e-156
Identity = 276/296 (93.24%), Postives = 282/296 (95.27%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MAVA FFP QTSYNN HYLSR+ +FR PLRLST R   ST+RASS LVLESD G QQDQN
Sbjct: 1   MAVATFFPSQTSYNNSHYLSRNIQFRYPLRLSTPRSFLSTIRASSALVLESDSGTQQDQN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
           SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY
Sbjct: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVS+LYERGWRQNFN+SGFPGLDEEFKMAMEYFKSVEGGLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSYLYERGWRQNFNQSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDA LLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRE 297
           GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWY+RF RE
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYLRFLRE 296

BLAST of Tan0021194.2 vs. ExPASy TrEMBL
Match: A0A6J1CMF3 (uncharacterized methyltransferase At2g41040, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111012967 PE=4 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 3.3e-156
Identity = 276/296 (93.24%), Postives = 285/296 (96.28%), Query Frame = 0

Query: 1   MAVAIFFPPQTSYNNRHYLSRDFRFRSPLRLSTRRCVSSTVRASSTLVLESDLGIQQDQN 60
           MAVA  FP QTSYNNR YLSRD RFRSPLRLSTRR   S +RASSTLVLESDLGIQQDQN
Sbjct: 1   MAVATLFPSQTSYNNRRYLSRDSRFRSPLRLSTRRSF-SFIRASSTLVLESDLGIQQDQN 60

Query: 61  SKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEY 120
           SK+D+FSCPVC+EPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVT+GM++Y
Sbjct: 61  SKVDLFSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTAGMRDY 120

Query: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASC 180
           VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPG DEEFKMAMEYFKS EGGLLVDASC
Sbjct: 121 VEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSAEGGLLVDASC 180

Query: 181 GSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240
           GSGLFSRKFAKSGSFSGV+ALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS
Sbjct: 181 GSGLFSRKFAKSGSFSGVVALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSS 240

Query: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRE 297
           GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSR+
Sbjct: 241 GSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRQ 295

BLAST of Tan0021194.2 vs. TAIR 10
Match: AT2G41040.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 370.5 bits (950), Expect = 1.2e-102
Identity = 189/286 (66.08%), Postives = 222/286 (77.62%), Query Frame = 0

Query: 14  NNRHYLSRDFRFRSP-LRLSTRRCVSSTVRASSTLVLESDLGIQQDQNSKID-----VFS 73
           N   + S D   +SP LR S    V S   +++   +     I +++  KI+     VF+
Sbjct: 23  NRSSFFSSD--SQSPLLRFSASTSVRSRFPSAAISAVAPKSDINKNETPKIEIEETQVFA 82

Query: 74  CPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEYVEVKPGG 133
           CPVC+EPL+RKGP G NL AIYRSGFKC +CNK+Y+SK+ +LDLTVT+ + +Y EVKP  
Sbjct: 83  CPVCYEPLMRKGPSGINLQAIYRSGFKCGQCNKTYSSKDEYLDLTVTADLDDYNEVKPIT 142

Query: 134 TELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASCGSGLFSR 193
           TELFRSPLVSFLYERGWRQ F RSGFPG DEEF+MA EYFK  EGGLLVD SCGSGLFSR
Sbjct: 143 TELFRSPLVSFLYERGWRQAFKRSGFPGPDEEFRMAEEYFKEAEGGLLVDVSCGSGLFSR 202

Query: 194 KFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNS-NLALVRADISRLPFSSGSVDAV 253
           KFA+SG +SGVIALD+SENML QC +FIK D    NS N+A+VRAD+SRLPF SGSVDAV
Sbjct: 203 KFAQSGKYSGVIALDYSENMLRQCKEFIKNDNTFDNSTNIAVVRADVSRLPFPSGSVDAV 262

Query: 254 HAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIR 293
           HAGAALHCWPSP+NAIAEI R++RSGGVFVGTTFLRY+ STPW IR
Sbjct: 263 HAGAALHCWPSPTNAIAEICRVLRSGGVFVGTTFLRYSPSTPWIIR 306

BLAST of Tan0021194.2 vs. TAIR 10
Match: AT1G78140.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 257.7 bits (657), Expect = 1.2e-68
Identity = 136/261 (52.11%), Postives = 169/261 (64.75%), Query Frame = 0

Query: 37  VSSTVRASSTLVLESDLGIQQDQNSKID--------------VFSCPVCFEPLL-RKGPP 96
           VS +  ASS     + + ++ + NS +D              + +CP+C+  L     P 
Sbjct: 38  VSRSAHASS-----ASVSVETNSNSNVDFVIEKKDKNRGEKKILACPICYNSLAWISQPN 97

Query: 97  GFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKEYVEVKPGGTELFRSPLVSFLYE 156
           G    A      +C  C +SY+     LDL V SG K Y E  P  TELFR+PLVSFLYE
Sbjct: 98  GLIESAASGIQVQCNTCKRSYSGNETHLDLAVASGSKRYSEPMPLSTELFRTPLVSFLYE 157

Query: 157 RGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDASCGSGLFSRKFAKSGSFSGVIAL 216
           RGWRQNF   GFPG ++EF+MA  Y K V GG ++DASCGSG+FSR F +S  FS VIAL
Sbjct: 158 RGWRQNFIWGGFPGPEKEFEMAKAYLKPVLGGNIIDASCGSGMFSRLFTRSDLFSLVIAL 217

Query: 217 DFSENMLLQCYDFIKKDAALLN-SNLALVRADISRLPFSSGSVDAVHAGAALHCWPSPSN 276
           D+SENML QCY+ + K+    N   L LVRADI+RLPF SGSVDAVHAGAALHCWPSPS+
Sbjct: 218 DYSENMLRQCYELLNKEENFPNKEKLVLVRADIARLPFLSGSVDAVHAGAALHCWPSPSS 277

Query: 277 AIAEITRIMRSGGVFVGTTFL 282
           A+AEI+R++R GGVFV TTF+
Sbjct: 278 AVAEISRVLRPGGVFVATTFI 293

BLAST of Tan0021194.2 vs. TAIR 10
Match: AT5G13710.1 (sterol methyltransferase 1 )

HSP 1 Score: 43.1 bits (100), Expect = 4.5e-04
Identity = 42/149 (28.19%), Postives = 70/149 (46.98%), Query Frame = 0

Query: 135 LVSFLYERGWRQNFN---RSGFPGLDEEFKMAMEYFKSVEGGL-----LVDASCGSGLFS 194
           L +  YE GW ++F+   R     L E  K   E+F +++ G+     ++D  CG G   
Sbjct: 51  LATSFYEYGWGESFHFAQRWKGESLRESIK-RHEHFLALQLGIQPGQKVLDVGCGIGGPL 110

Query: 195 RKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSSGSVDAV 254
           R+ A+  S S V  L+ +E  + +  +  +   A ++     V+AD  ++PF   S DAV
Sbjct: 111 REIARF-SNSVVTGLNNNEYQITRGKELNR--LAGVDKTCNFVKADFMKMPFPENSFDAV 170

Query: 255 HAGAALHCWPSPSNAIAEITRIMRSGGVF 276
           +A  A    P       EI R+++ G  F
Sbjct: 171 YAIEATCHAPDAYGCYKEIYRVLKPGQCF 195

BLAST of Tan0021194.2 vs. TAIR 10
Match: AT5G13710.2 (sterol methyltransferase 1 )

HSP 1 Score: 43.1 bits (100), Expect = 4.5e-04
Identity = 42/149 (28.19%), Postives = 70/149 (46.98%), Query Frame = 0

Query: 135 LVSFLYERGWRQNFN---RSGFPGLDEEFKMAMEYFKSVEGGL-----LVDASCGSGLFS 194
           L +  YE GW ++F+   R     L E  K   E+F +++ G+     ++D  CG G   
Sbjct: 51  LATSFYEYGWGESFHFAQRWKGESLRESIK-RHEHFLALQLGIQPGQKVLDVGCGIGGPL 110

Query: 195 RKFAKSGSFSGVIALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFSSGSVDAV 254
           R+ A+  S S V  L+ +E  + +  +  +   A ++     V+AD  ++PF   S DAV
Sbjct: 111 REIARF-SNSVVTGLNNNEYQITRGKELNR--LAGVDKTCNFVKADFMKMPFPENSFDAV 170

Query: 255 HAGAALHCWPSPSNAIAEITRIMRSGGVF 276
           +A  A    P       EI R+++ G  F
Sbjct: 171 YAIEATCHAPDAYGCYKEIYRVLKPGQCF 195

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0WPT71.7e-10166.08Uncharacterized methyltransferase At2g41040, chloroplastic OS=Arabidopsis thalia... [more]
Q8LBV41.6e-6752.11Uncharacterized methyltransferase At1g78140, chloroplastic OS=Arabidopsis thalia... [more]
Q5HP748.0e-0633.33Demethylmenaquinone methyltransferase OS=Staphylococcus epidermidis (strain ATCC... [more]
Q8CSH98.0e-0633.33Demethylmenaquinone methyltransferase OS=Staphylococcus epidermidis (strain ATCC... [more]
O143211.8e-0527.81Sterol 24-C-methyltransferase erg6 OS=Schizosaccharomyces pombe (strain 972 / AT... [more]
Match NameE-valueIdentityDescription
XP_038889838.11.8e-15995.27uncharacterized methyltransferase At2g41040, chloroplastic [Benincasa hispida][more]
KGN60753.14.0e-15693.24hypothetical protein Csa_019482 [Cucumis sativus][more]
XP_004139262.14.0e-15693.24uncharacterized methyltransferase At2g41040, chloroplastic [Cucumis sativus][more]
XP_008456627.15.3e-15693.24PREDICTED: uncharacterized methyltransferase At2g41040, chloroplastic isoform X1... [more]
XP_008456635.15.3e-15693.24PREDICTED: uncharacterized methyltransferase At2g41040, chloroplastic isoform X2... [more]
Match NameE-valueIdentityDescription
A0A0A0LG502.0e-15693.24Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G00... [more]
A0A5A7VAW12.6e-15693.24Putative methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3C4D92.6e-15693.24uncharacterized methyltransferase At2g41040, chloroplastic isoform X2 OS=Cucumis... [more]
A0A1S3C3A22.6e-15693.24uncharacterized methyltransferase At2g41040, chloroplastic isoform X1 OS=Cucumis... [more]
A0A6J1CMF33.3e-15693.24uncharacterized methyltransferase At2g41040, chloroplastic OS=Momordica charanti... [more]
Match NameE-valueIdentityDescription
AT2G41040.11.2e-10266.08S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G78140.11.2e-6852.11S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G13710.14.5e-0428.19sterol methyltransferase 1 [more]
AT5G13710.24.5e-0428.19sterol methyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013216Methyltransferase type 11PFAMPF08241Methyltransf_11coord: 177..276
e-value: 8.3E-18
score: 64.8
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 131..299
e-value: 6.7E-28
score: 99.6
NoneNo IPR availablePANTHERPTHR43591:SF66SUBFAMILY NOT NAMEDcoord: 24..292
NoneNo IPR availablePANTHERPTHR43591METHYLTRANSFERASEcoord: 24..292
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 175..281
e-value: 1.6621E-11
score: 58.2102
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 139..291

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Tan0021194Tan0021194gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Tan0021194.2-five_prime_utrTan0021194.2-five_prime_utr-LG08:3158207..3158294five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Tan0021194.2-exonTan0021194.2-exon-LG08:3158207..3158447exon
Tan0021194.2-exonTan0021194.2-exon-LG08:3158770..3158870exon
Tan0021194.2-exonTan0021194.2-exon-LG08:3158964..3159104exon
Tan0021194.2-exonTan0021194.2-exon-LG08:3159310..3159391exon
Tan0021194.2-exonTan0021194.2-exon-LG08:3159602..3159795exon
Tan0021194.2-exonTan0021194.2-exon-LG08:3159948..3160059exon
Tan0021194.2-exonTan0021194.2-exon-LG08:3160627..3162510exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Tan0021194.2-cdsTan0021194.2-cds-LG08:3158295..3158447CDS
Tan0021194.2-cdsTan0021194.2-cds-LG08:3158770..3158870CDS
Tan0021194.2-cdsTan0021194.2-cds-LG08:3158964..3159104CDS
Tan0021194.2-cdsTan0021194.2-cds-LG08:3159310..3159391CDS
Tan0021194.2-cdsTan0021194.2-cds-LG08:3159602..3159795CDS
Tan0021194.2-cdsTan0021194.2-cds-LG08:3159948..3160059CDS
Tan0021194.2-cdsTan0021194.2-cds-LG08:3160627..3160743CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Tan0021194.2-three_prime_utrTan0021194.2-three_prime_utr-LG08:3160744..3162510three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Tan0021194.2Tan0021194.2-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0006744 ubiquinone biosynthetic process
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0050661 NADP binding
molecular_function GO:0004499 N,N-dimethylaniline monooxygenase activity