Homology
BLAST of Spg028666 vs. NCBI nr
Match:
XP_038877934.1 (RNA-dependent RNA polymerase 1-like isoform X1 [Benincasa hispida] >XP_038877936.1 RNA-dependent RNA polymerase 1-like isoform X1 [Benincasa hispida] >XP_038877937.1 RNA-dependent RNA polymerase 1-like isoform X1 [Benincasa hispida] >XP_038877938.1 RNA-dependent RNA polymerase 1-like isoform X1 [Benincasa hispida])
HSP 1 Score: 2032.7 bits (5265), Expect = 0.0e+00
Identity = 983/1115 (88.16%), Postives = 1042/1115 (93.45%), Query Frame = 0
Query: 1 MGKTIQLYGFPPGVLQEAVKAFVERFTGEGTIDAIKIKRSKGRGRRVYATIQFTDDEGAK 60
MGKTIQL+GFP GVLQE+VK FVERFTG+GTIDAI KRSKGRGRR+YA IQFTD+EGAK
Sbjct: 1 MGKTIQLFGFPSGVLQESVKTFVERFTGKGTIDAINTKRSKGRGRRMYAIIQFTDEEGAK 60
Query: 61 LIISLANRRLCYDTSYLKAREWKQDILPDPLVFEYNFKGLRLHLGCQISKERFSVLWTES 120
IIS A L Y TSYLKAREWK DILPDP VFEYNFKGLRLHLGCQISKE FS+LWTES
Sbjct: 61 SIISKATEGLFYGTSYLKAREWKHDILPDPPVFEYNFKGLRLHLGCQISKESFSMLWTES 120
Query: 121 TVSVDFGFERRELKFFISHPHVDYMLVLRYENIWQVELHKPQGQSVDYLLIQLFGAPRIY 180
VSVDFGFE+R+L FFIS+PHVDYMLVLRYENIWQVELHKPQGQSVDYLLIQLFGAPRIY
Sbjct: 121 NVSVDFGFEQRKLYFFISYPHVDYMLVLRYENIWQVELHKPQGQSVDYLLIQLFGAPRIY 180
Query: 181 ERDTKSYRPITEDPFFTFSEEIDTKWFRATDFTPSCCIGQSAALCLEIPYGRQLPNFHDN 240
ERD S I DPF FS E+DT+WFRATDFTPSCCIGQSAA+CLEIPYGRQLPNFHD
Sbjct: 181 ERDAISLGHIIGDPFLNFSMEVDTQWFRATDFTPSCCIGQSAAICLEIPYGRQLPNFHDK 240
Query: 241 FAYYKEIKGKLTLVSGSTYSSNVNLVPVVRPPLNINLPYAILFKINLLVQQGCLPGPALD 300
FAY+KE GK +LVSGSTYSSNVNLVPVVRPPL INLPYAILFKINLLVQQGCLPGPALD
Sbjct: 241 FAYFKEFNGKFSLVSGSTYSSNVNLVPVVRPPLTINLPYAILFKINLLVQQGCLPGPALD 300
Query: 301 ISFYQMVDPQIHNIAFIDHALKKLYHLKECCYNPSKWLDDEYRKYFRLKNPPLPPIFTLN 360
I+FYQMVDP+I+NIAFIDHALKKL HLKECCYNPSKWLD+EYRKYF+ KNPP PPI +L+
Sbjct: 301 INFYQMVDPRIYNIAFIDHALKKLLHLKECCYNPSKWLDEEYRKYFKSKNPPQPPILSLS 360
Query: 361 EGLVYVHRVQVTPCKVYFCGPEVNVSNRVLRGYPKYLDNFLRVSFVDEELDKMYSTELSP 420
EGLVYVHRVQVTPCKVYFCGPEVNVSNRVLR YP YLDNFLRVSFVDEELDKMYSTELSP
Sbjct: 361 EGLVYVHRVQVTPCKVYFCGPEVNVSNRVLRRYPDYLDNFLRVSFVDEELDKMYSTELSP 420
Query: 421 RESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDGLTAAK 480
R SSSLEDGKTKIFKRILSVL+DGITIGDKKFEFLAYSSSQLRENA WMFAPRDGLTAA+
Sbjct: 421 RASSSLEDGKTKIFKRILSVLRDGITIGDKKFEFLAYSSSQLRENAAWMFAPRDGLTAAR 480
Query: 481 IRKGMGDFHSIRNVAKYAARLGQSFGSSTETLSVSRREVEVIPDIEVESGSGVNYVFSDG 540
IR+ MGDFH IRNVAKYAARLGQSFGSSTETLSVSRREV +IPDIEVESG GVNYVFSDG
Sbjct: 481 IRRWMGDFHCIRNVAKYAARLGQSFGSSTETLSVSRREVNLIPDIEVESGGGVNYVFSDG 540
Query: 541 IGKISASFARKVAQKCGIRHTPSAFQIRYAGFKGVISIDPTSSVKLSLRNSMLKYESADT 600
IGKIS SFA+KVA+KCGIRH PSAFQIRYAGFKGVIS+DPTSSVKLSLRNSMLKYES DT
Sbjct: 541 IGKISVSFAKKVAKKCGIRHMPSAFQIRYAGFKGVISVDPTSSVKLSLRNSMLKYESTDT 600
Query: 601 KLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQKELIEQLDTIFTDPMKAQQALEL 660
KLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQ+ELI++LDTIF+DPMKAQ+ALEL
Sbjct: 601 KLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQRELIDELDTIFSDPMKAQRALEL 660
Query: 661 MSPGENTNILKEMILCGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPNGRAMMGCLD 720
MSPGENT ILKEM+LCGYRPDSEPFL MMLHTFRESKLLELRRKSRIFIPNGRAM+GCLD
Sbjct: 661 MSPGENTKILKEMMLCGYRPDSEPFLWMMLHTFRESKLLELRRKSRIFIPNGRAMLGCLD 720
Query: 721 ETGNLDYGEVFVQCSAHQKLHNDHIIFRRSKSNRHFIVTGTVVVAKNPCLHPGDVRVLTA 780
ET NL+YGEVFVQCSAHQ+L +DHIIF+R KSNRHFIVTGTVVVAKNPCLHPGDVRVLTA
Sbjct: 721 ETRNLEYGEVFVQCSAHQQLRDDHIIFKRRKSNRHFIVTGTVVVAKNPCLHPGDVRVLTA 780
Query: 781 VDVPSLHHMVDCVVFPQRGSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP 840
VDVP+LHHMVDCVVFPQ+GSRPHPNECSGSDLDGDIYFVCWDPDLIPP QVEPMDYTPVP
Sbjct: 781 VDVPALHHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDPDLIPPLQVEPMDYTPVP 840
Query: 841 SKLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADREPEKAMSKTCIKLAKLFSIAV 900
SKLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFAD+EPEKAMS CI+LAKLFSIAV
Sbjct: 841 SKLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADKEPEKAMSNPCIELAKLFSIAV 900
Query: 901 DFPKTGVPALISADLRVQAYPDFMDKADKVSYKSDNVLGKLFRMLDNVGPKIK-IRSLTF 960
DFPKTG+PALI A+LRVQ YPDFMDKADKV+Y+S NVLGKLFRMLDN+GP IK IRS +
Sbjct: 901 DFPKTGIPALIPANLRVQEYPDFMDKADKVTYESKNVLGKLFRMLDNIGPNIKNIRSFAY 960
Query: 961 TREEAQQAYDPDMEVEGFEEYLDDAFYHKNNYDMRLGNLMNYYKIKTEAELISGGSLTKS 1020
T + A++AYDPDMEVEGFEEYLDDA YHKNNYDMRLGNLM+YYKIKTEAELISGGSLT S
Sbjct: 961 TPDVAREAYDPDMEVEGFEEYLDDAIYHKNNYDMRLGNLMHYYKIKTEAELISGGSLTSS 1020
Query: 1021 LSFTKKNEAESITMAVKSLRKEARGWFNENAELHFGHDKNVYARASAWYFVTYHHSYWGW 1080
LSFTKKNEAESITMAVKSLRKEARGWFNENA+LH+GHD NVYARASAWYFVTYHH+YWGW
Sbjct: 1021 LSFTKKNEAESITMAVKSLRKEARGWFNENADLHYGHDTNVYARASAWYFVTYHHTYWGW 1080
Query: 1081 YDDGKKHGHFLSFPWCVHDKLIRIKKRKMNARARY 1115
D K HGHFLSFPWC++DKLIRIK RK N+RARY
Sbjct: 1081 SDGRKNHGHFLSFPWCIYDKLIRIKNRKTNSRARY 1115
BLAST of Spg028666 vs. NCBI nr
Match:
XP_022943176.1 (RNA-dependent RNA polymerase 1-like [Cucurbita moschata])
HSP 1 Score: 2018.8 bits (5229), Expect = 0.0e+00
Identity = 980/1115 (87.89%), Postives = 1042/1115 (93.45%), Query Frame = 0
Query: 1 MGKTIQLYGFPPGVLQEAVKAFVERFTGEGTIDAIKIKRSKGRGRRVYATIQFTDDEGAK 60
MGKTIQLYGFP GVLQE+VKAFVERFTGEG+IDAI +KRS+GRG+R+YA IQF DD GAK
Sbjct: 1 MGKTIQLYGFPSGVLQESVKAFVERFTGEGSIDAINVKRSRGRGKRLYAIIQFIDDVGAK 60
Query: 61 LIISLANRRLCYDTSYLKAREWKQDILPDPLVFEYNFKGLRLHLGCQISKERFSVLWTES 120
LIISLAN L Y TSYLKAREWKQDIL DPLVFEYNFKGL LHLGCQIS+E F VLW ES
Sbjct: 61 LIISLANASLRYGTSYLKAREWKQDILQDPLVFEYNFKGLELHLGCQISREEFFVLWRES 120
Query: 121 TVSVDFGFERRELKFFISHPHVDYMLVLRYENIWQVELHKPQGQSVDYLLIQLFGAPRIY 180
VSV+F FERR+LKFFISHP VDYMLVLRYENIWQVELHKPQGQS+DYLLIQL GAPRIY
Sbjct: 121 NVSVNFEFERRDLKFFISHPRVDYMLVLRYENIWQVELHKPQGQSIDYLLIQLVGAPRIY 180
Query: 181 ERDTKSYRPITEDPFFTFSEEIDTKWFRATDFTPSCCIGQSAALCLEIPYGRQLPNFHDN 240
ERDTK + I+EDPF +FS+E+DTKWFRATDFTPSCCIGQS ALCLEIPYGR LPNFHD
Sbjct: 181 ERDTKLFGHISEDPFLSFSKEMDTKWFRATDFTPSCCIGQSGALCLEIPYGRLLPNFHDK 240
Query: 241 FAYYKEIKGKLTLVSGSTYSSNVNLVPVVRPPLNINLPYAILFKINLLVQQGCLPGPALD 300
FAY+ EIKGK TL SGSTYSSNVNLVPVVRPPL+I+LPYAILFKINLLVQQGCLPGP+LD
Sbjct: 241 FAYFNEIKGKFTLASGSTYSSNVNLVPVVRPPLSIHLPYAILFKINLLVQQGCLPGPSLD 300
Query: 301 ISFYQMVDPQIHNIAFIDHALKKLYHLKECCYNPSKWLDDEYRKYFRLKNPPLPPIFTLN 360
ISFY+MVDPQIHNIAFID AL+KLY LKECCYNPSKWL +EYRKY +L NPP PP +L+
Sbjct: 301 ISFYEMVDPQIHNIAFIDRALRKLYDLKECCYNPSKWLGEEYRKYSKLTNPPQPPNLSLD 360
Query: 361 EGLVYVHRVQVTPCKVYFCGPEVNVSNRVLRGYPKYLDNFLRVSFVDEELDKMYSTELSP 420
EGLVYVHRVQVTPC+VYFCGPEVNVSNRVLR YP+YLDNFLRVSFVDEELDK+YSTELSP
Sbjct: 361 EGLVYVHRVQVTPCRVYFCGPEVNVSNRVLRRYPEYLDNFLRVSFVDEELDKLYSTELSP 420
Query: 421 RESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDGLTAAK 480
RESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDG+TAAK
Sbjct: 421 RESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDGITAAK 480
Query: 481 IRKGMGDFHSIRNVAKYAARLGQSFGSSTETLSVSRREVEVIPDIEVESGSGVNYVFSDG 540
IRK MGDFHSIRNVAKYAARLGQSFGSSTET SVSRREV+VIPDIEV+S +G+NYVFSDG
Sbjct: 481 IRKWMGDFHSIRNVAKYAARLGQSFGSSTETASVSRREVKVIPDIEVKSDAGINYVFSDG 540
Query: 541 IGKISASFARKVAQKCGIRHTPSAFQIRYAGFKGVISIDPTSSVKLSLRNSMLKYESADT 600
IGKISASFAR+VA+KC IRHTPSAFQIRYAGFKGVIS+DPTS VKLSLRNSMLKYES DT
Sbjct: 541 IGKISASFAREVARKCHIRHTPSAFQIRYAGFKGVISVDPTSFVKLSLRNSMLKYESTDT 600
Query: 601 KLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQKELIEQLDTIFTDPMKAQQALEL 660
KLDVLSWS+YHPC+LNRQLITLLSTLGVQD +FESKQKELI+QLDTIF DP+KAQQALEL
Sbjct: 601 KLDVLSWSRYHPCYLNRQLITLLSTLGVQDDIFESKQKELIDQLDTIFMDPLKAQQALEL 660
Query: 661 MSPGENTNILKEMILCGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPNGRAMMGCLD 720
MSPGENTNILKE+I CGYRPDSEPFL MMLHTFRESKLLELRRKSRIFIPNGRAMMGCLD
Sbjct: 661 MSPGENTNILKEIISCGYRPDSEPFLYMMLHTFRESKLLELRRKSRIFIPNGRAMMGCLD 720
Query: 721 ETGNLDYGEVFVQCSAHQKLHNDHIIFRRSKSNRHFIVTGTVVVAKNPCLHPGDVRVLTA 780
ETGNL+YGEVFVQCSAHQ+LHNDHIIF+RSKSN+HFIVTGTVVVAKNPCLHPGDVRVLTA
Sbjct: 721 ETGNLEYGEVFVQCSAHQQLHNDHIIFKRSKSNQHFIVTGTVVVAKNPCLHPGDVRVLTA 780
Query: 781 VDVPSLHHMVDCVVFPQRGSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP 840
VDVPSLHHMVDC+VFPQ+G RPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP
Sbjct: 781 VDVPSLHHMVDCLVFPQKGPRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP 840
Query: 841 SKLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADREPEKAMSKTCIKLAKLFSIAV 900
S+LLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFAD PEKAMS+ CIKLAKLFSIAV
Sbjct: 841 SRLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADSRPEKAMSEPCIKLAKLFSIAV 900
Query: 901 DFPKTGVPALISADLRVQAYPDFMDKADKVSYKSDNVLGKLFRMLDNVGPKIKIRSLTFT 960
DFPKTGVPALISADLRVQ YPDFMDK DK SYKS NVLGKLFRMLD++ P+I I+S+ FT
Sbjct: 901 DFPKTGVPALISADLRVQVYPDFMDKPDKQSYKSHNVLGKLFRMLDDIEPRISIKSVKFT 960
Query: 961 REEAQQAYDPDMEVEGFEEYLDDAFYHKNNYDMRLGNLMNYYKIKTEAELISGGSLTKSL 1020
RE A+QAYDPDMEV+GFEEYLDDA YHKNNYDMRLGNLM YYKIKTEAELISG SLTKS
Sbjct: 961 REIARQAYDPDMEVKGFEEYLDDALYHKNNYDMRLGNLMKYYKIKTEAELISGVSLTKSS 1020
Query: 1021 SFTKKNEAESITMAVKSLRKEARGWFNENAELHFGHDKNVYARASAWYFVTYHHSYWGWY 1080
SFTKKNEAESI MAVKSLRKEARGWFNENA LHFGHD N YARASAWYFVTYH+SYWGWY
Sbjct: 1021 SFTKKNEAESIIMAVKSLRKEARGWFNENAGLHFGHDNNAYARASAWYFVTYHYSYWGWY 1080
Query: 1081 DDGKKHGHFLSFPWCVHDKLIRIKKRKMNARARYR 1116
+ K HFLSFPWCV+DKLIRIKKRK+ ARAR+R
Sbjct: 1081 GE-KNESHFLSFPWCVYDKLIRIKKRKIGARARHR 1114
BLAST of Spg028666 vs. NCBI nr
Match:
XP_023539816.1 (RNA-dependent RNA polymerase 1-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 2016.9 bits (5224), Expect = 0.0e+00
Identity = 980/1115 (87.89%), Postives = 1041/1115 (93.36%), Query Frame = 0
Query: 1 MGKTIQLYGFPPGVLQEAVKAFVERFTGEGTIDAIKIKRSKGRGRRVYATIQFTDDEGAK 60
MGKTIQLYGFP GVLQE+VKAFVERFTGEG+IDAI +KRS+GRG+R+YA IQF DD GAK
Sbjct: 1 MGKTIQLYGFPSGVLQESVKAFVERFTGEGSIDAINVKRSRGRGKRLYAIIQFIDDVGAK 60
Query: 61 LIISLANRRLCYDTSYLKAREWKQDILPDPLVFEYNFKGLRLHLGCQISKERFSVLWTES 120
LIISLAN L Y TSYLKAREWKQDIL DPLVFEYNFKGL LHLGCQIS+E F VLW ES
Sbjct: 61 LIISLANASLRYGTSYLKAREWKQDILQDPLVFEYNFKGLELHLGCQISREEFFVLWRES 120
Query: 121 TVSVDFGFERRELKFFISHPHVDYMLVLRYENIWQVELHKPQGQSVDYLLIQLFGAPRIY 180
VSV+F FERR+LKFFISH H DYMLVLRYENIWQVELHKP GQS+DYLLIQL GAPRIY
Sbjct: 121 NVSVNFEFERRDLKFFISHSHADYMLVLRYENIWQVELHKPPGQSIDYLLIQLVGAPRIY 180
Query: 181 ERDTKSYRPITEDPFFTFSEEIDTKWFRATDFTPSCCIGQSAALCLEIPYGRQLPNFHDN 240
ERDTK + I+EDPF +FS+E+DT+WFRATDFTPSCCIGQS ALCLEIPYGR LPNFHD
Sbjct: 181 ERDTKLFGHISEDPFLSFSKEMDTEWFRATDFTPSCCIGQSGALCLEIPYGRLLPNFHDK 240
Query: 241 FAYYKEIKGKLTLVSGSTYSSNVNLVPVVRPPLNINLPYAILFKINLLVQQGCLPGPALD 300
FAY+KEIKGK TL SGSTYSSNVNLVPVVRPPL+I+LPYAILFKINLLVQQGCLPGPALD
Sbjct: 241 FAYFKEIKGKFTLASGSTYSSNVNLVPVVRPPLSIHLPYAILFKINLLVQQGCLPGPALD 300
Query: 301 ISFYQMVDPQIHNIAFIDHALKKLYHLKECCYNPSKWLDDEYRKYFRLKNPPLPPIFTLN 360
ISFY+MVDPQIHNIAFID AL+KLY LKECCYNPSKWL +EYRKY +L NPP PP +L+
Sbjct: 301 ISFYEMVDPQIHNIAFIDRALRKLYDLKECCYNPSKWLGEEYRKYSKLTNPPQPPNLSLD 360
Query: 361 EGLVYVHRVQVTPCKVYFCGPEVNVSNRVLRGYPKYLDNFLRVSFVDEELDKMYSTELSP 420
EGLVYVHRVQVTPC+VYFCGPEVNVSNRVLR YP+YLDNFLRVSFVDEELDK+YSTELSP
Sbjct: 361 EGLVYVHRVQVTPCRVYFCGPEVNVSNRVLRRYPEYLDNFLRVSFVDEELDKLYSTELSP 420
Query: 421 RESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDGLTAAK 480
RESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDG+TAAK
Sbjct: 421 RESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDGITAAK 480
Query: 481 IRKGMGDFHSIRNVAKYAARLGQSFGSSTETLSVSRREVEVIPDIEVESGSGVNYVFSDG 540
IRK MGDFHSIRNVAKYAARLGQSFGSSTET SVSRREVEVIPDIEV+S +G+NYVFSDG
Sbjct: 481 IRKWMGDFHSIRNVAKYAARLGQSFGSSTETASVSRREVEVIPDIEVKSDAGINYVFSDG 540
Query: 541 IGKISASFARKVAQKCGIRHTPSAFQIRYAGFKGVISIDPTSSVKLSLRNSMLKYESADT 600
IGKISASFAR+VA+KC IRHTPSAFQIRYAGFKGVIS+DPTS VKLSLRNSMLKYES DT
Sbjct: 541 IGKISASFAREVARKCRIRHTPSAFQIRYAGFKGVISVDPTSFVKLSLRNSMLKYESTDT 600
Query: 601 KLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQKELIEQLDTIFTDPMKAQQALEL 660
KLDVLSWS+YHPC+LNRQLITLLSTLGVQD +FESKQKELI+QLDTIF DP+KAQQALEL
Sbjct: 601 KLDVLSWSRYHPCYLNRQLITLLSTLGVQDDIFESKQKELIDQLDTIFMDPLKAQQALEL 660
Query: 661 MSPGENTNILKEMILCGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPNGRAMMGCLD 720
MSPGENTNILKE+I CGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPNGRAMMGCLD
Sbjct: 661 MSPGENTNILKEIISCGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPNGRAMMGCLD 720
Query: 721 ETGNLDYGEVFVQCSAHQKLHNDHIIFRRSKSNRHFIVTGTVVVAKNPCLHPGDVRVLTA 780
ETGNL+YGEVFVQCSAHQ+LHNDHIIF+RSKSN+HFIVTGTVVVAKNPCLHPGDVRVLTA
Sbjct: 721 ETGNLEYGEVFVQCSAHQQLHNDHIIFKRSKSNQHFIVTGTVVVAKNPCLHPGDVRVLTA 780
Query: 781 VDVPSLHHMVDCVVFPQRGSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP 840
VDVPSLHHMVDC+VFPQ+G RPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPV
Sbjct: 781 VDVPSLHHMVDCLVFPQKGPRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVL 840
Query: 841 SKLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADREPEKAMSKTCIKLAKLFSIAV 900
S+LLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFAD PEKAMS+ CIKLAKLFSIAV
Sbjct: 841 SRLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADSRPEKAMSEPCIKLAKLFSIAV 900
Query: 901 DFPKTGVPALISADLRVQAYPDFMDKADKVSYKSDNVLGKLFRMLDNVGPKIKIRSLTFT 960
DFPKTGVPALISADLRVQ YPDFMDK DK SYKS NVLGKLFRMLD++ P+I I+S+ FT
Sbjct: 901 DFPKTGVPALISADLRVQVYPDFMDKPDKQSYKSHNVLGKLFRMLDDIEPRISIKSVKFT 960
Query: 961 REEAQQAYDPDMEVEGFEEYLDDAFYHKNNYDMRLGNLMNYYKIKTEAELISGGSLTKSL 1020
RE A+QAYDPDMEV+GFEEYLDDA YHKNNYDMRLGNLM YYKIKTEAELISG SLTKS
Sbjct: 961 REIARQAYDPDMEVDGFEEYLDDALYHKNNYDMRLGNLMKYYKIKTEAELISGVSLTKSS 1020
Query: 1021 SFTKKNEAESITMAVKSLRKEARGWFNENAELHFGHDKNVYARASAWYFVTYHHSYWGWY 1080
SFTKKNEAESI MAVKSLRKEARGWFNENA LHFGHD N YARASAWYFVTYH+SYWGWY
Sbjct: 1021 SFTKKNEAESIIMAVKSLRKEARGWFNENAGLHFGHDNNAYARASAWYFVTYHYSYWGWY 1080
Query: 1081 DDGKKHGHFLSFPWCVHDKLIRIKKRKMNARARYR 1116
+ K HFLSFPWCV+DKLIRIKKRK+ ARAR+R
Sbjct: 1081 GE-KNESHFLSFPWCVYDKLIRIKKRKIGARARHR 1114
BLAST of Spg028666 vs. NCBI nr
Match:
KAG6599932.1 (RNA-dependent RNA polymerase 1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 2016.9 bits (5224), Expect = 0.0e+00
Identity = 980/1115 (87.89%), Postives = 1043/1115 (93.54%), Query Frame = 0
Query: 1 MGKTIQLYGFPPGVLQEAVKAFVERFTGEGTIDAIKIKRSKGRGRRVYATIQFTDDEGAK 60
MGKTIQLYGFP GVLQE+VKAFVERFTGEG+IDAI +KRS+GRG+R+YA IQF DD GAK
Sbjct: 1 MGKTIQLYGFPSGVLQESVKAFVERFTGEGSIDAINVKRSRGRGKRLYAIIQFIDDVGAK 60
Query: 61 LIISLANRRLCYDTSYLKAREWKQDILPDPLVFEYNFKGLRLHLGCQISKERFSVLWTES 120
LIISLAN L Y TSYLKAREWKQDIL DPLVFEYNFKGL LHLGCQIS+E F VLW ES
Sbjct: 61 LIISLANASLRYGTSYLKAREWKQDILQDPLVFEYNFKGLELHLGCQISREEFFVLWRES 120
Query: 121 TVSVDFGFERRELKFFISHPHVDYMLVLRYENIWQVELHKPQGQSVDYLLIQLFGAPRIY 180
VSV+F FERR+LKFFISHP VDYMLVLRYENIWQVELHKPQGQS+DYLLIQL GAPRIY
Sbjct: 121 NVSVNFEFERRDLKFFISHPRVDYMLVLRYENIWQVELHKPQGQSIDYLLIQLVGAPRIY 180
Query: 181 ERDTKSYRPITEDPFFTFSEEIDTKWFRATDFTPSCCIGQSAALCLEIPYGRQLPNFHDN 240
ERDTK + I+EDPF +FS+E+DTKWFRATDFTPSCCIGQS ALCLEIPYGR LPNFHD
Sbjct: 181 ERDTKLFGHISEDPFLSFSKEMDTKWFRATDFTPSCCIGQSGALCLEIPYGRLLPNFHDK 240
Query: 241 FAYYKEIKGKLTLVSGSTYSSNVNLVPVVRPPLNINLPYAILFKINLLVQQGCLPGPALD 300
FAY+KEIKGK TL SGSTYSSNVNLVPVVRPPL+I+LPYAILFKINLLVQQGCLPGP+LD
Sbjct: 241 FAYFKEIKGKFTLASGSTYSSNVNLVPVVRPPLSIHLPYAILFKINLLVQQGCLPGPSLD 300
Query: 301 ISFYQMVDPQIHNIAFIDHALKKLYHLKECCYNPSKWLDDEYRKYFRLKNPPLPPIFTLN 360
ISFY+MVDPQIHNIAFID AL+KLY LKECCYNPSKWL +EYRKY +L+NPP PP +L+
Sbjct: 301 ISFYEMVDPQIHNIAFIDRALRKLYDLKECCYNPSKWLGEEYRKYSKLRNPPQPPNLSLD 360
Query: 361 EGLVYVHRVQVTPCKVYFCGPEVNVSNRVLRGYPKYLDNFLRVSFVDEELDKMYSTELSP 420
EGLVYVHRVQVTPC+VYFCGPEVNVSNRVLR YP+YLDNFLRVSFVDEELDK+YSTELSP
Sbjct: 361 EGLVYVHRVQVTPCRVYFCGPEVNVSNRVLRRYPEYLDNFLRVSFVDEELDKLYSTELSP 420
Query: 421 RESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDGLTAAK 480
RESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPR+G+TAAK
Sbjct: 421 RESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRNGITAAK 480
Query: 481 IRKGMGDFHSIRNVAKYAARLGQSFGSSTETLSVSRREVEVIPDIEVESGSGVNYVFSDG 540
IRK MGDFHSIRNVAKYAARLGQSFGSSTET SVSRREVEVIPDIEV+S +G+NYVFSDG
Sbjct: 481 IRKWMGDFHSIRNVAKYAARLGQSFGSSTETASVSRREVEVIPDIEVKSDAGINYVFSDG 540
Query: 541 IGKISASFARKVAQKCGIRHTPSAFQIRYAGFKGVISIDPTSSVKLSLRNSMLKYESADT 600
IGKISASFAR+VA+KC IRHTPSAFQIRYAGFKGVIS+DPTS VKLSLRNSMLKYES DT
Sbjct: 541 IGKISASFAREVARKCRIRHTPSAFQIRYAGFKGVISVDPTSFVKLSLRNSMLKYESTDT 600
Query: 601 KLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQKELIEQLDTIFTDPMKAQQALEL 660
KLDVLSWS+YHPC+LNRQLITLLSTLGVQD +FESKQKELI+QLDTIF DP+KAQQALEL
Sbjct: 601 KLDVLSWSRYHPCYLNRQLITLLSTLGVQDDIFESKQKELIDQLDTIFMDPLKAQQALEL 660
Query: 661 MSPGENTNILKEMILCGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPNGRAMMGCLD 720
MSPGENTNILKE+I CGYRPDSEPFL MML+TFRESKLLELRRKSRIFIPNGRAMMGCLD
Sbjct: 661 MSPGENTNILKEIISCGYRPDSEPFLYMMLYTFRESKLLELRRKSRIFIPNGRAMMGCLD 720
Query: 721 ETGNLDYGEVFVQCSAHQKLHNDHIIFRRSKSNRHFIVTGTVVVAKNPCLHPGDVRVLTA 780
ETGNL+YGEVFVQCSAHQ+LHNDHIIF+RSKSN+HFIVTGTVVVAKNPCLHPGDVRVLTA
Sbjct: 721 ETGNLEYGEVFVQCSAHQQLHNDHIIFKRSKSNQHFIVTGTVVVAKNPCLHPGDVRVLTA 780
Query: 781 VDVPSLHHMVDCVVFPQRGSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP 840
VDVPSLHHMVDC+VFPQ+G RPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPV
Sbjct: 781 VDVPSLHHMVDCLVFPQKGPRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVL 840
Query: 841 SKLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADREPEKAMSKTCIKLAKLFSIAV 900
S+LLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFAD PEKAMS+ CIKLAKLFSIAV
Sbjct: 841 SRLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADSRPEKAMSEPCIKLAKLFSIAV 900
Query: 901 DFPKTGVPALISADLRVQAYPDFMDKADKVSYKSDNVLGKLFRMLDNVGPKIKIRSLTFT 960
DFPKTGVPALISADLRVQ YPDFMDK DK SYKS NVLGKLFRMLD++ P+I I+S+ FT
Sbjct: 901 DFPKTGVPALISADLRVQVYPDFMDKPDKQSYKSHNVLGKLFRMLDDIEPRISIKSVKFT 960
Query: 961 REEAQQAYDPDMEVEGFEEYLDDAFYHKNNYDMRLGNLMNYYKIKTEAELISGGSLTKSL 1020
RE A+QAYDPDMEVEGFEEYLDDA YHKNNYDMRLGNLM YYKIKTEAELISG SLTKS
Sbjct: 961 REIARQAYDPDMEVEGFEEYLDDALYHKNNYDMRLGNLMKYYKIKTEAELISGVSLTKSS 1020
Query: 1021 SFTKKNEAESITMAVKSLRKEARGWFNENAELHFGHDKNVYARASAWYFVTYHHSYWGWY 1080
SFTKKNEAESI MAVKSLRKEARGWFNENA LHFGHD N YARASAWYFVTYH+SYWGWY
Sbjct: 1021 SFTKKNEAESIIMAVKSLRKEARGWFNENAGLHFGHDNNAYARASAWYFVTYHYSYWGWY 1080
Query: 1081 DDGKKHGHFLSFPWCVHDKLIRIKKRKMNARARYR 1116
+ K HFLSFPWCV+DKLIRIKKRK+ ARAR+R
Sbjct: 1081 GE-KNESHFLSFPWCVYDKLIRIKKRKIGARARHR 1114
BLAST of Spg028666 vs. NCBI nr
Match:
NP_001267682.1 (RNA-dependent RNA polymerase 1-like [Cucumis sativus] >XP_011654424.1 RNA-dependent RNA polymerase 1-like isoform X1 [Cucumis sativus] >ADU77016.1 RNA-dependent RNA polymerase 1b [Cucumis sativus] >KGN50762.1 hypothetical protein Csa_005874 [Cucumis sativus])
HSP 1 Score: 2007.6 bits (5200), Expect = 0.0e+00
Identity = 974/1115 (87.35%), Postives = 1035/1115 (92.83%), Query Frame = 0
Query: 1 MGKTIQLYGFPPGVLQEAVKAFVERFTGEGTIDAIKIKRSKGRGRRVYATIQFTDDEGAK 60
MGKTIQL+GFP GVLQE+VK FVE TG GTIDAI KRSKG GRRVYA IQFTD+EGAK
Sbjct: 1 MGKTIQLFGFPSGVLQESVKTFVEGITGTGTIDAINTKRSKGGGRRVYAIIQFTDEEGAK 60
Query: 61 LIISLANRRLCYDTSYLKAREWKQDILPDPLVFEYNFKGLRLHLGCQISKERFSVLWTES 120
IIS A RLCY TSYLKARE K DILPDPLVF+YNFK LRLHLGCQISKE FSVLWTES
Sbjct: 61 SIISKATERLCYGTSYLKAREMKHDILPDPLVFDYNFKALRLHLGCQISKESFSVLWTES 120
Query: 121 TVSVDFGFERRELKFFISHPHVDYMLVLRYENIWQVELHKPQGQSVDYLLIQLFGAPRIY 180
VSVDFGFE R+L FFIS+P VDYMLVLRYENIWQVELHKP GQSVDYLLIQLFGAPRIY
Sbjct: 121 NVSVDFGFELRKLYFFISYPRVDYMLVLRYENIWQVELHKPHGQSVDYLLIQLFGAPRIY 180
Query: 181 ERDTKSYRPITEDPFFTFSEEIDTKWFRATDFTPSCCIGQSAALCLEIPYGRQLPNFHDN 240
ERD +S+ ITEDPF FS EIDT+WFRATDFTPSC IGQSAALCLEIPYGRQLPNFHD
Sbjct: 181 ERDARSFGLITEDPFLNFSTEIDTQWFRATDFTPSCSIGQSAALCLEIPYGRQLPNFHDK 240
Query: 241 FAYYKEIKGKLTLVSGSTYSSNVNLVPVVRPPLNINLPYAILFKINLLVQQGCLPGPALD 300
FAY+KEIKGK TLVSGSTYSSNVNLVPVV PP INLPY ILFKINLLVQQGCLPGPALD
Sbjct: 241 FAYFKEIKGKFTLVSGSTYSSNVNLVPVVTPPRTINLPYTILFKINLLVQQGCLPGPALD 300
Query: 301 ISFYQMVDPQIHNIAFIDHALKKLYHLKECCYNPSKWLDDEYRKYFRLKNPPLPPIFTLN 360
ISFYQMVD QI+N A IDHALKKL HLKECCYNPSKWLD+EYRKYF+LKNPP PPI TLN
Sbjct: 301 ISFYQMVDSQIYNTAVIDHALKKLLHLKECCYNPSKWLDEEYRKYFKLKNPPQPPILTLN 360
Query: 361 EGLVYVHRVQVTPCKVYFCGPEVNVSNRVLRGYPKYLDNFLRVSFVDEELDKMYSTELSP 420
EGLVYVHRVQVTPCKVYFCGPEVN+SNRVLR YP Y+DNFLRVSFVDEEL KMYSTELSP
Sbjct: 361 EGLVYVHRVQVTPCKVYFCGPEVNISNRVLRRYPDYIDNFLRVSFVDEELGKMYSTELSP 420
Query: 421 RESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDGLTAAK 480
R SSSLEDGKTKIFKRILSVL+DGITIGDKKFEFLAYSSSQLRENA WMFAP++ LTAAK
Sbjct: 421 RASSSLEDGKTKIFKRILSVLRDGITIGDKKFEFLAYSSSQLRENAAWMFAPKNELTAAK 480
Query: 481 IRKGMGDFHSIRNVAKYAARLGQSFGSSTETLSVSRREVEVIPDIEVESGSGVNYVFSDG 540
IR+ MGDFH+IRNVAKYAARLGQSFGSSTETLSVSRREV+VIPDIEVESGSGVNYVFSDG
Sbjct: 481 IRQWMGDFHNIRNVAKYAARLGQSFGSSTETLSVSRREVKVIPDIEVESGSGVNYVFSDG 540
Query: 541 IGKISASFARKVAQKCGIRHTPSAFQIRYAGFKGVISIDPTSSVKLSLRNSMLKYESADT 600
IGKI+ASFARKVA+KCGIRHTPSAFQIRYAGFKGVIS+DPTSSVKLSLRNSMLKYES DT
Sbjct: 541 IGKIAASFARKVAKKCGIRHTPSAFQIRYAGFKGVISVDPTSSVKLSLRNSMLKYESTDT 600
Query: 601 KLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQKELIEQLDTIFTDPMKAQQALEL 660
KLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQ+ELI++LDTIF+DP+KAQQALEL
Sbjct: 601 KLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQQELIDELDTIFSDPLKAQQALEL 660
Query: 661 MSPGENTNILKEMILCGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPNGRAMMGCLD 720
MSPGENT ILKEM+LCGY+PDSEPFL MMLHTFRESKL+ELR KSRIFIPNGRAMMGCLD
Sbjct: 661 MSPGENTKILKEMMLCGYKPDSEPFLRMMLHTFRESKLMELRMKSRIFIPNGRAMMGCLD 720
Query: 721 ETGNLDYGEVFVQCSAHQKLHNDHIIFRRSKSNRHFIVTGTVVVAKNPCLHPGDVRVLTA 780
ET NL+YGEVFVQCSAHQ+LH+D +IF+R KSNRHFIVTGTVVVAKNPCLHPGDVRVLTA
Sbjct: 721 ETRNLEYGEVFVQCSAHQQLHDDRVIFKRIKSNRHFIVTGTVVVAKNPCLHPGDVRVLTA 780
Query: 781 VDVPSLHHMVDCVVFPQRGSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP 840
VDVPSLHHM+DCVVFPQ+GSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP
Sbjct: 781 VDVPSLHHMIDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP 840
Query: 841 SKLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADREPEKAMSKTCIKLAKLFSIAV 900
S++LDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFAD+EP+KAMS CI+LAKLFSIAV
Sbjct: 841 SQVLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADKEPKKAMSNPCIQLAKLFSIAV 900
Query: 901 DFPKTGVPALISADLRVQAYPDFMDKADKVSYKSDNVLGKLFRMLDNVGPKIK-IRSLTF 960
DFPKTGVPALI A+LRV+ YPDFMDKADKV+Y+S+NVLGKLFRMLD++GP IK IRS +
Sbjct: 901 DFPKTGVPALIPANLRVKEYPDFMDKADKVTYESENVLGKLFRMLDSIGPNIKNIRSFNY 960
Query: 961 TREEAQQAYDPDMEVEGFEEYLDDAFYHKNNYDMRLGNLMNYYKIKTEAELISGGSLTKS 1020
T E A+Q YDPDMEVEGFEEYLDDA YHKNNYDMRLGNLM+Y+KIKTEAELISGGSLT S
Sbjct: 961 TPEMARQDYDPDMEVEGFEEYLDDAIYHKNNYDMRLGNLMHYHKIKTEAELISGGSLTSS 1020
Query: 1021 LSFTKKNEAESITMAVKSLRKEARGWFNENAELHFGHDKNVYARASAWYFVTYHHSYWGW 1080
LSFT KNEAESI +AVKSLRKEARGWFNE A+LH+GH NVYARASAWYFVTYHH+YWGW
Sbjct: 1021 LSFTMKNEAESIILAVKSLRKEARGWFNEKADLHYGHHTNVYARASAWYFVTYHHTYWGW 1080
Query: 1081 YDDGKKHGHFLSFPWCVHDKLIRIKKRKMNARARY 1115
D K HGHFLSFPWCV+DKLIRIK RK+N RARY
Sbjct: 1081 SDGRKNHGHFLSFPWCVYDKLIRIKHRKINCRARY 1115
BLAST of Spg028666 vs. ExPASy Swiss-Prot
Match:
Q9LQV2 (RNA-dependent RNA polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=RDR1 PE=2 SV=1)
HSP 1 Score: 1355.5 bits (3507), Expect = 0.0e+00
Identity = 680/1117 (60.88%), Postives = 841/1117 (75.29%), Query Frame = 0
Query: 1 MGKTIQLYGFPPGVLQEAVKAFVERFTGEGTIDAIKIKRSKGRGRRVYATIQFTDDEGAK 60
MGKTIQ++GFP GV E VK F+ER TG GT+ AIK+++ K G RVYA +QFT + +
Sbjct: 1 MGKTIQVFGFPNGVSAEEVKKFLERLTGSGTVYAIKVRQPKKGGPRVYAIVQFTSERHTR 60
Query: 61 LIISLANRRLCYDTSYLKAREWKQDILPDPLVFEYNFKGLRLHLGCQISKERFSVLWTES 120
LII+ A RL Y SYLKA E +QDI+P P + GL++ GCQ+S ++F LW+
Sbjct: 61 LIITAAAERLYYGRSYLKAFEVEQDIVPKPRASLHTISGLKMFFGCQVSTKKFLTLWSAQ 120
Query: 121 TVSVDFGFERRELKFFISHPHVDYMLVLRYENIWQVELHKPQGQSVDYLLIQLFGAPRIY 180
V V FG R+L F S DY L L YENIWQ++LH PQG+S +L+IQ+ GAP+I+
Sbjct: 121 DVCVSFGIGMRKLHFSFSWYQKDYRLELSYENIWQIDLHSPQGRSSKFLVIQVIGAPKIF 180
Query: 181 ERDTKSYRPITEDPFFTFSEEIDTKWFRATDFTPSCCIGQSAALCLEIPYGRQLPNFHDN 240
E++ + + F +S+ D +W R TDFT S CIGQS A CLE+P +P+F +N
Sbjct: 181 EKEDQPINLLFGIMDF-YSDGSDEQWIRTTDFTSSSCIGQSTAFCLELPVHLNVPDFREN 240
Query: 241 FAYYKEIKGKLTLV-SGSTYSSNVN-LVPVVRPPLNINLPYAILFKINLLVQQGCLPGPA 300
FA Y E + L+ SGS+YSSN N LVPVV PP +LP+ ILFK+N LVQ CL GPA
Sbjct: 241 FANYAEHRASSFLIESGSSYSSNANTLVPVVDPPPGFSLPFEILFKLNTLVQNACLSGPA 300
Query: 301 LDISFYQMVDPQIHNIAFIDHALKKLYHLKECCYNPSKWLDDEYRKYFRLKNPPLPPIFT 360
LD+ FY++++ + ++ A IDH L+KL+HL ECCY P+ WL DEY+K+ PL P +
Sbjct: 301 LDLDFYRLLNQKKYDRALIDHCLEKLFHLGECCYEPAHWLRDEYKKWISKGKLPLSPTIS 360
Query: 361 LNEGLVYVHRVQVTPCKVYFCGPEVNVSNRVLRGYPKYLDNFLRVSFVDEELDKMYSTEL 420
L++GLVY++RVQVTP +VYF GPEVNVSNRVLR Y KY++NFLRVSFVDE+L+K+ S +L
Sbjct: 361 LDDGLVYMYRVQVTPARVYFSGPEVNVSNRVLRHYSKYINNFLRVSFVDEDLEKVRSMDL 420
Query: 421 SPRESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDGLTA 480
SPR S+ +TK++ RI SVL+DGI IGDKKFEFLA+SSSQLREN+ WMFAP D +TA
Sbjct: 421 SPRSST---QRRTKLYDRIYSVLRDGIVIGDKKFEFLAFSSSQLRENSAWMFAPIDRITA 480
Query: 481 AKIRKGMGDFHSIRNVAKYAARLGQSFGSSTETLSVSRREVEVIPDIEVESGSGVNYVFS 540
A IR MGDF IRNVAKYAARLGQSF SS ETL+V E+EVIPD+E+ S G YVFS
Sbjct: 481 AHIRAWMGDFDHIRNVAKYAARLGQSFSSSRETLNVRSDEIEVIPDVEIIS-LGTRYVFS 540
Query: 541 DGIGKISASFARKVAQKCGI-RHTPSAFQIRYAGFKGVISIDPTSSVKLSLRNSMLKYES 600
DGIGKISA FARKVA+KCG+ +PSAFQIRY G+KGV+++DP SS KLSLR SM K+ES
Sbjct: 541 DGIGKISAEFARKVARKCGLTEFSPSAFQIRYGGYKGVVAVDPNSSKKLSLRKSMSKFES 600
Query: 601 ADTKLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQKELIEQLDTIFTDPMKAQQA 660
+TKLDVL+WSKY PC++NRQLITLLSTLGV D VFE KQ+E++++LD I T P++A +A
Sbjct: 601 ENTKLDVLAWSKYQPCYMNRQLITLLSTLGVTDSVFEKKQREVVDRLDAILTHPLEAHEA 660
Query: 661 LELMSPGENTNILKEMILCGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPNGRAMMG 720
L LM+PGENTNILK +ILCGY+PD+EPFLSMML FR SKLLELR K+RIFI GR+MMG
Sbjct: 661 LGLMAPGENTNILKALILCGYKPDAEPFLSMMLQNFRASKLLELRTKTRIFISGGRSMMG 720
Query: 721 CLDETGNLDYGEVFVQCSAHQKLHNDHIIFRRSKSNRHFIVTGTVVVAKNPCLHPGDVRV 780
CLDET L+YG+V VQ S + R FI+TG VVVAKNPCLHPGDVRV
Sbjct: 721 CLDETRTLEYGQVVVQYSDPM------------RPGRRFIITGPVVVAKNPCLHPGDVRV 780
Query: 781 LTAVDVPSLHHMVDCVVFPQRGSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYT 840
L AV+VP+L+HMVDCVVFPQ+G RPHPNECSGSDLDGDIYFVCWD +L+PP+ EPMDYT
Sbjct: 781 LQAVNVPALNHMVDCVVFPQKGLRPHPNECSGSDLDGDIYFVCWDQELVPPRTSEPMDYT 840
Query: 841 PVPSKLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADREPEKAMSKTCIKLAKLFS 900
P P+++LDHDVT+EEV+EYFANY+VNDSLGIIANAHTAFAD+EP KA S CI+LAK FS
Sbjct: 841 PEPTQILDHDVTIEEVEEYFANYIVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKFS 900
Query: 901 IAVDFPKTGVPALISADLRVQAYPDFMDKADKVSYKSDNVLGKLFR-MLDNVGPKIKIRS 960
AVDFPKTGV A+I L V+ YPDFM+K DK +Y+S NV+GKLFR + + P I I+S
Sbjct: 901 TAVDFPKTGVAAVIPQHLYVKEYPDFMEKPDKPTYESKNVIGKLFREVKERAPPLISIKS 960
Query: 961 LTFTREEAQQAYDPDMEVEGFEEYLDDAFYHKNNYDMRLGNLMNYYKIKTEAELISGGSL 1020
FT + A ++YD DMEV+GFEEY+D+AFY K NYD +LGNLM+YY IKTEAE++SGG +
Sbjct: 961 --FTLDVASKSYDKDMEVDGFEEYVDEAFYQKANYDFKLGNLMDYYGIKTEAEILSGGIM 1020
Query: 1021 TKSLSFTKKNEAESITMAVKSLRKEARGWFNENAELHFGHDKNVYARASAWYFVTYHHSY 1080
S SFTK+ +AESI AV++LRKE FN + E ++N A+ASAWY VTYH SY
Sbjct: 1021 RMSKSFTKRRDAESIGRAVRALRKETLSLFNASEE-----EENESAKASAWYHVTYHSSY 1080
Query: 1081 WGWYDDGKKHGHFLSFPWCVHDKLIRIKKRKMNARAR 1114
WG Y++G HFLSF WCV+DKL+RIKK + R R
Sbjct: 1081 WGLYNEGLNRDHFLSFAWCVYDKLVRIKKTNLGRRQR 1093
BLAST of Spg028666 vs. ExPASy Swiss-Prot
Match:
Q0DXS3 (Probable RNA-dependent RNA polymerase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR1 PE=2 SV=2)
HSP 1 Score: 904.8 bits (2337), Expect = 9.5e-262
Identity = 454/739 (61.43%), Postives = 574/739 (77.67%), Query Frame = 0
Query: 376 VYFCGPEVNVSNRVLRGYPKYLDNFLRVSFVDEELDKMYSTELSPRESSSLEDGKTKIFK 435
VYF GPE+NVSNRV+R + ++NFLR+SFVDE+ +K+ +T+LSPR +S + +T ++K
Sbjct: 9 VYFYGPEINVSNRVVRNFSSDIENFLRISFVDEDCEKLRATDLSPRSASGHDANRTALYK 68
Query: 436 RILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDGLTAAKIRKGMGDFHSIRNVA 495
R+LSVL DGITIG K FEFLA+SSSQLR+N+ WMFA R GL A+ IR MGDF +IRNVA
Sbjct: 69 RVLSVLSDGITIGGKNFEFLAFSSSQLRDNSAWMFASRQGLAASDIRTWMGDFRNIRNVA 128
Query: 496 KYAARLGQSFGSSTETLSVSRREVEVIPDIEVESGSGVNYVFSDGIGKISASFARKVAQK 555
KYAARLGQSF SSTETL V + EVE I DI+ +G +VFSDGIGKIS++FA +VA K
Sbjct: 129 KYAARLGQSFSSSTETLKVQKYEVEEISDIK----NGTQHVFSDGIGKISSAFANEVAMK 188
Query: 556 CGI-RHTPSAFQIRYAGFKGVISIDPTSSVKLSLRNSMLKYESADTKLDVLSWSKYHPCF 615
C + R PSAFQIRY G+KGV+++DPTS KLSLR SMLK++S + +DVL++SKY P F
Sbjct: 189 CNLKRFAPSAFQIRYGGYKGVVAVDPTSRWKLSLRKSMLKFQSDNITVDVLAYSKYQPGF 248
Query: 616 LNRQLITLLSTLGVQDHVFESKQKELIEQLDTIFTDPMKAQQALELMSPGENTNILKEMI 675
LNRQLITLLSTLGV+D VFE KQ+E + QL+ + TDP A +A+ELM GE TN +KE++
Sbjct: 249 LNRQLITLLSTLGVRDSVFEQKQEEAVNQLNKMVTDPQAAIEAIELMPMGEITNAVKELL 308
Query: 676 LCGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPNGRAMMGCLDETGNLDYGEVFVQC 735
LCGY+PD EP+LSM+L TFR SKLLEL+ KSRI IP GRAMMGCLDET L YG+VF++
Sbjct: 309 LCGYQPDDEPYLSMLLQTFRASKLLELKTKSRILIPKGRAMMGCLDETRTLKYGQVFIRA 368
Query: 736 SAHQKLHNDHIIFRRSKSNRHFIVTGTVVVAKNPCLHPGDVRVLTAVDVPSLHHMVDCVV 795
++ ND N F VTG VV+AKNPCLHPGD+R+L AVDVP LHHM +CVV
Sbjct: 369 TSGV---ND---------NDRFTVTGKVVIAKNPCLHPGDIRILHAVDVPVLHHMFNCVV 428
Query: 796 FPQRGSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVPSKLLDHDVTMEEVQ 855
FPQ+G RPHPNECSGSDLDGDIYFV WDP LIPP+ V PMDYTP P++ LDHDVT+EEV+
Sbjct: 429 FPQQGPRPHPNECSGSDLDGDIYFVSWDPSLIPPRMVTPMDYTPAPTETLDHDVTIEEVE 488
Query: 856 EYFANYMVNDSLGIIANAHTAFADREPEKAMSKTCIKLAKLFSIAVDFPKTGVPALISAD 915
EYF NY+VN+SLG+IANAH FAD+E KA S CI+LAKLFSIAVDFPKTGVPALI +
Sbjct: 489 EYFTNYIVNESLGMIANAHVVFADKEDLKAESSPCIELAKLFSIAVDFPKTGVPALIPPE 548
Query: 916 LRVQAYPDFMDKADKVSYKSDNVLGKLFRMLDNVGPKIKIRSLTFTREEAQQAYDPDMEV 975
L V+ YPDFM+K DKV+Y+S V+GKL+R + P IK FTRE A+++YD DM V
Sbjct: 549 LHVKEYPDFMEKLDKVTYESKGVIGKLYREIKKHTPHIK----HFTREVARRSYDTDMIV 608
Query: 976 EGFEEYLDDAFYHKNNYDMRLGNLMNYYKIKTEAELISGGSLTKSLSFTKKNEAESITMA 1035
+G+E+Y+ +A K+ YD +LGNLM++Y IK+EAE+ISG L + +FTKK++A++I +A
Sbjct: 609 DGYEDYITEAMALKDEYDFKLGNLMDHYGIKSEAEIISGCILKMAKNFTKKSDADAIRLA 668
Query: 1036 VKSLRKEARGWFNENA--ELHFGHDKNVYARASAWYFVTYHHSYWGWYDDGKKHGHFLSF 1095
V+SLRKEAR F+E + + GHD + A+ASAWY VTYH +WG Y++G + HF+SF
Sbjct: 669 VRSLRKEARSRFSEMSLDDNGHGHDAS-EAKASAWYHVTYHPEFWGCYNEGYERPHFISF 726
Query: 1096 PWCVHDKLIRIKKRKMNAR 1112
PWC+++KL+RIK+R+ R
Sbjct: 729 PWCIYEKLLRIKQRRKFVR 726
BLAST of Spg028666 vs. ExPASy Swiss-Prot
Match:
O82504 (RNA-dependent RNA polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=RDR2 PE=1 SV=1)
HSP 1 Score: 761.1 bits (1964), Expect = 1.7e-218
Identity = 435/1125 (38.67%), Postives = 666/1125 (59.20%), Query Frame = 0
Query: 4 TIQLYGFPPGVLQEAVKAFVERFTGEGTIDAIKIKRSKGRGR-RVYATIQFTDDE-GAKL 63
T+++ P ++ + + F+E GE T+ A++I ++ + R +A +QFT E ++
Sbjct: 11 TVKISNVPQTIVADELLRFLELHLGEDTVFALEIPTTRDNWKPRDFARVQFTTLEVKSRA 70
Query: 64 IISLANRRLCYDTSYLKAREWKQDILPDPLVFEYNFKGLRLHLGCQISKE-RFSVLWTES 123
+ + +L + T L+ E DI+P P+ + L +G S E RF L
Sbjct: 71 QLLSSQSKLLFKTHNLRLSEAYDDIIPRPVDPRKRLDDIVLTVGFPESDEKRFCALEKWD 130
Query: 124 TVSVDFGFERRELKFFISHPHVDYMLVLRYENIWQVELHKPQGQS--VDYLLIQLFGAPR 183
V E+R ++F++ Y + +R+E+I + G + +D L++L P+
Sbjct: 131 GVRCWILTEKRRVEFWVWESGDCYKIEVRFEDIIETLSCCVNGDASEIDAFLLKLKYGPK 190
Query: 184 IYERDTKSYRPITEDPFFTF-SEEIDTKWFRATDFTPSCCIGQSAALCLEIPYGRQLPNF 243
+++R T + + F E+ D W R TDF+ S IG S CLE+ G + +
Sbjct: 191 VFKRVTVHIATKFKSDRYRFCKEDFDFMWIRTTDFSGSKSIGTSTCFCLEVHNGSTMLDI 250
Query: 244 HDNFAYYKEIKGKLTLVSGSTYSSNVNLVPVVRPP-LNINLPYAILFKINLLVQ-QGCLP 303
YY+E LT V G T++S +VP++ L + PY ILF++N LV Q
Sbjct: 251 FSGLPYYREDTLSLTYVDGKTFASAAQIVPLLNAAILGLEFPYEILFQLNALVHAQKISL 310
Query: 304 GPALDISFYQMVDPQIHNIAFIDHALKKLYHLKECCYNPSKWLDDEYRKYF-RLKNPPLP 363
A D+ +++ A + LKKL+ CY+P ++ + + ++K+ P
Sbjct: 311 FAASDMELIKILRGMSLETALV--ILKKLHQQSSICYDPVFFVKTQMQSVVKKMKHSPAS 370
Query: 364 PIFTLNE-GLVYVHRVQVTPCKVYFCGPEVNVSNRVLRGYPKYLDNFLRVSFVDEELDKM 423
L E ++ R VTP K+Y GPE+ +N V++ + +++ +F+RV+FV+E+ K+
Sbjct: 371 AYKRLTEQNIMSCQRAYVTPSKIYLLGPELETANYVVKNFAEHVSDFMRVTFVEEDWSKL 430
Query: 424 YSTELS--PRESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFA 483
+ LS +E ++ +T I+ R+LS+L +GIT+G K+FEFLA+S+SQLR N+VWMFA
Sbjct: 431 PANALSVNSKEGYFVKPSRTNIYNRVLSILGEGITVGPKRFEFLAFSASQLRGNSVWMFA 490
Query: 484 PRDGLTAAKIRKGMGDFHSIRNVAKYAARLGQSFGSSTETLSVSRREVEVIPDIEVESGS 543
+ + A IR+ MG F IR+++K AAR+GQ F +S +TL V ++VE IPDIEV +
Sbjct: 491 SNEKVKAEDIREWMGCFRKIRSISKCAARMGQLFSASRQTLIVRAQDVEQIPDIEVTT-D 550
Query: 544 GVNYVFSDGIGKISASFARKVAQKCGIRHTPSAFQIRYAGFKGVISIDPTSSVKLSLRNS 603
G +Y FSDGIGKIS +FA++VAQKCG+ H PSAFQIRY G+KGVI++D +S KLSLR+S
Sbjct: 551 GADYCFSDGIGKISLAFAKQVAQKCGLSHVPSAFQIRYGGYKGVIAVDRSSFRKLSLRDS 610
Query: 604 MLKYESADTKLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQKELIEQLDTIFTDP 663
MLK++S + L+V W++ PCFLNR++I LLSTLG++D +FE+ Q + L + D
Sbjct: 611 MLKFDSNNRMLNVTRWTESMPCFLNREIICLLSTLGIEDAMFEAMQAVHLSMLGNMLEDR 670
Query: 664 MKAQQALELMSPGENTNILKEMILCGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPN 723
A L+ +S + N+L +M+L GY P SEP+LSMML ES+L EL+ + RI +P
Sbjct: 671 DAALNVLQKLSGENSKNLLVKMLLQGYAPSSEPYLSMMLRVHHESQLSELKSRCRILVPK 730
Query: 724 GRAMMGCLDETGNLDYGEVFVQCS-AHQKLHNDHIIFRRSKSNRHFIVTGTVVVAKNPCL 783
GR ++GC+DE G L+YG+V+V+ + +L + + R +V G VVV KNPCL
Sbjct: 731 GRILIGCMDEMGILEYGQVYVRVTLTKAELKSRDQSYFRKIDEETSVVIGKVVVTKNPCL 790
Query: 784 HPGDVRVLTAVDVPSLHH--MVDCVVFPQRGSRPHPNECSGSDLDGDIYFVCWDPDLIPP 843
HPGD+RVL A+ +DC++FPQ+G RPHPNECSG DLDGD +FV WD +IP
Sbjct: 791 HPGDIRVLDAIYEVHFEEKGYLDCIIFPQKGERPHPNECSGGDLDGDQFFVSWDEKIIPS 850
Query: 844 QQVEPMDYTPVPSKLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADREPEKAMSKT 903
+ PMDY +L+DHDVT+EE+ ++F +YM++D+LG+I+ AH ADR+PEKA S+
Sbjct: 851 EMDPPMDYAGSRPRLMDHDVTLEEIHKFFVDYMISDTLGVISTAHLVHADRDPEKARSQK 910
Query: 904 CIKLAKLFSIAVDFPKTGVPALISADLRVQAYPDFMDKADKVSYKSDNVLGKLFRMLDNV 963
C++LA L S AVDF KTG PA + L+ + +PDF+++ +K +Y S++V GKL+R + +
Sbjct: 911 CLELANLHSRAVDFAKTGAPAEMPYALKPREFPDFLERFEKPTYISESVFGKLYRAVKS- 970
Query: 964 GPKIKIRSLTFTREEAQQ----AYDPDMEVEGFEEYLDDAFYHKNNYDMRLGNLMNYYKI 1023
SL + EA+ AYD +E GFE +++ A H++ Y +L +LM YY
Sbjct: 971 -------SLAQRKPEAESEDTVAYDVTLEEAGFESFIETAKAHRDMYGEKLTSLMIYYGA 1030
Query: 1024 KTEAELISGGSLTKSLSFTKKNE-----AESITMAVKSLRKEARGWFNENAELHFGHDKN 1083
E E+++G TK + + N + IT++VK L KEA GWF ++ E ++
Sbjct: 1031 ANEEEILTGILKTKEMYLARDNRRYGDMKDRITLSVKDLHKEAMGWFEKSCE----DEQQ 1090
Query: 1084 VYARASAWYFVTYHHSYWGWYDDGKKHGHFLSFPWCVHDKLIRIK 1105
ASAWY+VTY+ + + D K FLSFPW V D L+ IK
Sbjct: 1091 KKKLASAWYYVTYNPN----HRDEKL--TFLSFPWIVGDVLLDIK 1114
BLAST of Spg028666 vs. ExPASy Swiss-Prot
Match:
Q7XM31 (Probable RNA-dependent RNA polymerase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR2 PE=2 SV=1)
HSP 1 Score: 724.2 bits (1868), Expect = 2.3e-207
Identity = 424/1136 (37.32%), Postives = 640/1136 (56.34%), Query Frame = 0
Query: 4 TIQLYGFPPGVLQEAVKAFVERFTG-EGTIDAIKIKRS-KGRGRRVYATIQFTDDEGAKL 63
++++ PP + + AF + G A +I + +G R + T+QF A
Sbjct: 11 SLRVSNIPPSAVAAELLAFFDSAVAVAGGAFACEIAAAHRGWLSRGHGTVQFGSAAAAAA 70
Query: 64 IISLAN--RRLCYDTSYLKAREWKQDILPDPLVFEYNFKGLRLHLGCQISKERFSVLWTE 123
LA+ R + + L D+LP G L +G ++++ F
Sbjct: 71 AAGLASSGRLPRFLGALLSVSPSPVDLLPRASDLSLRAAGAGLVVGDRVAERVFEAADAW 130
Query: 124 STVSVDFGFERRELKFFISHPHVDYMLVLRYENIWQVELHKPQGQSVDYLLIQLFGAPRI 183
V + +R + ++ H Y L + +E++ G +L+QL APRI
Sbjct: 131 DGVRAEVIPGKRRVDLYLEHDSQRYKLEVLFEDMKDCLGCTLDGMGA--ILLQLNYAPRI 190
Query: 184 YE--RDTKSYRPITEDPFFTFSEEIDTKWFRATDFTPSCCIGQSAALCLEIPYGRQLPNF 243
+ +D F E+ W RA DFTP+ G+ + L L++ + +
Sbjct: 191 HTAISGPAVNSRFMDDRFHACKEDAKFSWVRALDFTPNYSFGRCSTLVLKLGKSALVSDI 250
Query: 244 HDNFAYYKEIKGKLTLVSGSTYSSNVNLVPVVRPPLNINLPYAILFKINLLVQQGCLPGP 303
+ + + G+LT+ S ++ N+VP+V P + ++PY +LF++N L+ G +
Sbjct: 251 LKSLPFSGNL-GELTMNSMDGVGASSNVVPLVHCPRDYSVPYEVLFRLNSLMHMGKIVAK 310
Query: 304 ALDISFYQMVDPQIHNIAFIDHALKKLYHLKECCYNPSKWLDDEYRKYFRLKNPPLPPIF 363
++ ++ + +++ +K++ L+ CY P +++ E R N +
Sbjct: 311 HVNADLFKALQELPVDVS--RRIFEKMHKLESTCYGPLQFIQQEAYSMKRSHN-----VL 370
Query: 364 TLNEG-------LVYVHRVQVTPCKVYFCGPEVNVSNRVLRGYPKYLDNFLRVSFVDEEL 423
NEG L+ +RV +TP K++ GPE V+N V++ + Y +F+RV+FVDE+
Sbjct: 371 LSNEGEGEGERKLMKCYRVNITPSKIFCFGPEEEVTNYVVKHHSAYASDFVRVTFVDEDW 430
Query: 424 DKMYSTELSPRESSSL--EDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVW 483
K+ S +S R + KT ++ RILS+LK G +IG K FEFLA+S+SQLR N+VW
Sbjct: 431 SKLSSNAISARIEQGFFSKPFKTGLYYRILSILKKGFSIGPKNFEFLAFSASQLRGNSVW 490
Query: 484 MFAPRDGLTAAKIRKGMGDFHSIRNVAKYAARLGQSFGSSTETLSVSRREVEVIPDIEVE 543
MFA L A IR+ MG F +IR+V+K AAR+GQ F SS +T V R +VEVIPDIE+
Sbjct: 491 MFASNASLNAGGIRRWMGHFENIRSVSKCAARMGQLFSSSRQTFEVLRWDVEVIPDIEIT 550
Query: 544 SGSGVNYVFSDGIGKISASFARKVAQKCGIRHT--PSAFQIRYAGFKGVISIDPTSSVKL 603
+ G Y+FSDGIGKIS FA++VA G+ T PSAFQIRY G+KGVI+IDP SS+ L
Sbjct: 551 T-DGSKYIFSDGIGKISLRFAKRVAHHVGLDPTNLPSAFQIRYGGYKGVIAIDPMSSIDL 610
Query: 604 SLRNSMLKYESADTKLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQKELIEQLDT 663
SLR SM K+ES L++ SWSK PC++NR++I+LLSTLG++D +F + Q++ + + +
Sbjct: 611 SLRPSMKKFESESRMLNITSWSKSQPCYVNREIISLLSTLGIRDEIFVAMQQDEMRETEE 670
Query: 664 IFTDPMKAQQALELMSPGENTNILKEMILCGYRPDSEPFLSMMLHTFRESKLLELRRKSR 723
+ T+ A L + G T +M+L GY P SEP+LSM+L +E++L ++R + +
Sbjct: 671 MLTNKEVALSVLGKLG-GSETKTAVKMLLQGYEPSSEPYLSMILKAHQENRLTDIRTRCK 730
Query: 724 IFIPNGRAMMGCLDETGNLDYGEVFVQCSAHQKLHND-HIIFRRSKSNRHFIVTGTVVVA 783
I +P GR ++GCLDETG L+YG+V+++ + + K D + + + + V G V +
Sbjct: 731 IHVPKGRVLIGCLDETGVLEYGQVYIRITKNSKEQKDSNQSYFYNDDGKTATVVGKVAIT 790
Query: 784 KNPCLHPGDVRVLTAVDVPSLHHMVDCVVFPQRGSRPHPNECSGSDLDGDIYFVCWDPDL 843
KNPCLHPGD+RVL A+ P L MVDC+VFPQRG RPHPNECSG DLDGD+YF+ WD L
Sbjct: 791 KNPCLHPGDIRVLEAIYDPDLVGMVDCLVFPQRGERPHPNECSGGDLDGDLYFITWDDKL 850
Query: 844 IPPQQVEPMDYTPVPSKLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADREPEKAM 903
IP + PMDYT +++DH VT+EE+Q++F +YM+NDSLG I+ AH ADR P KA
Sbjct: 851 IPEKVDTPMDYTATRPRIMDHVVTLEEIQKHFVDYMINDSLGAISTAHLIHADRSPLKAR 910
Query: 904 SKTCIKLAKLFSIAVDFPKTGVPALISADLRVQAYPDFMDKADKVSYKSDNVLGKLFR-M 963
S C++LA L S+AVDF KTG PA + LR + YPDFM++ +K Y S+ VLGKL+R
Sbjct: 911 SPECLQLATLHSMAVDFAKTGAPAEMPRTLRPREYPDFMERWEKPMYISNGVLGKLYRSA 970
Query: 964 LDNVGPKIKIRSLTFTREEAQQAYDPDMEVEGFEEYLDDAFYHKNNYDMRLGNLMNYYKI 1023
+ ++ +L+ + + YDPD+EV G +E+L A + Y+ +L LMNYY+
Sbjct: 971 MGHMEKSGDSGALSSSSAQPSPTYDPDLEVPGSDEFLQAAEEYYELYEEKLTTLMNYYRA 1030
Query: 1024 KTEAELISGGSLTKSLSFTKKNE-----AESITMAVKSLRKEARGWFNENAELHFGHDKN 1083
+ E E+++G K L + N+ + I AV +L +EARGW L +++
Sbjct: 1031 ELEDEILTGNIRNKMLYLKRDNKRYFEMKDRIVAAVDALHREARGWL-----LSSRKEED 1090
Query: 1084 VYARASAWYFVTYHHSYWGWYDDGKKHGHFLSFPWCVHDKLIRIKKRKMNARARYR 1116
ASAWY VTYH D ++ F SFPW D L+ IK R R +
Sbjct: 1091 ASRMASAWYRVTYH-------PDRRRGKRFWSFPWIACDNLLAIKASSQLRRRRQK 1122
BLAST of Spg028666 vs. ExPASy Swiss-Prot
Match:
Q9SG02 (RNA-dependent RNA polymerase 6 OS=Arabidopsis thaliana OX=3702 GN=RDR6 PE=1 SV=1)
HSP 1 Score: 572.8 bits (1475), Expect = 8.6e-162
Identity = 386/1074 (35.94%), Postives = 570/1074 (53.07%), Query Frame = 0
Query: 95 YNFKGLRLHLGCQISKERFSVLWTESTV---------SVDFGFERR---ELKFFISHP-- 154
Y G+ L +G +S++ F V W V + F F + K + H
Sbjct: 130 YKLAGITLEIGTLVSRDDFFVSWRAEGVDFLVDPFDNTCKFCFRKSTAFSFKDAVMHAVI 189
Query: 155 HVDYMLVLRYENIWQVELHKPQGQSVDYLLIQLFGAPRIYERDTKSYRPITEDPFFTFSE 214
+ DY L L +I V +K V L++QL +PR++ YR +D + T
Sbjct: 190 NCDYKLELLVRDIQTVRQYKTLHGFV--LILQLASSPRVW------YRTADDDIYDTVPG 249
Query: 215 EI---DTKWFRATDFTPSCCIGQ--SAALCLEIPYGRQLPNFHDNFAYYKEIKGKLTLVS 274
++ D W R TDFT IG+ S + + Y +L D F + + ++
Sbjct: 250 DLLDDDDPWIRTTDFTQVGAIGRCHSYRVLISPRYENKLRTALDYFRMRRVQEERV---- 309
Query: 275 GSTYSSNVNLVPVVRPPLNIN---------LPYAILFKINLLVQQGCLPGPALDISFYQM 334
+ + P P++ + + + I+F +N ++ +G L F+ +
Sbjct: 310 --RWPPRIRNEPCFGEPVSDHFFCIHHKEGISFEIMFLVNSVLHRGVFNQFQLTERFFDL 369
Query: 335 V--DPQIHNIAFIDHALKKLYHLKECCYNPSKWLDDEYRKYFRLKNPPLPPIFTLNEGLV 394
+ P+ NIA +LK L K ++ K L + + + KNP L +E +
Sbjct: 370 LRNQPKDVNIA----SLKHLCTYKRPVFDAYKRL--KLVQEWIQKNPKLLGSHEQSEDIS 429
Query: 395 YVHRVQVTPCKVYFCGPEVNVSNRVLRGYPKYLDNFLRVSFVDEELDKMYSTELS----- 454
+ R+ +TP + Y PEV +SNRVLR Y + FLRV+F+DE + + S LS
Sbjct: 430 EIRRLVITPTRAYCLPPEVELSNRVLRRYKAVAERFLRVTFMDESMQTINSNVLSYFVAP 489
Query: 455 --PRESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDGLT 514
+SS KT +FKR+ S+L DG + +K+ FLA+S++QLR+ + W FA
Sbjct: 490 IVKDLTSSSFSQKTYVFKRVKSILTDGFKLCGRKYSFLAFSANQLRDRSAWFFAEDGKTR 549
Query: 515 AAKIRKGMGDFHSIRNVAKYAARLGQSFGSSTETLSVSRREVEV-IPDIEVESGSGVNYV 574
+ I+ MG F +NVAK AAR+G F S+ T+ V EV+ +PDIE YV
Sbjct: 550 VSDIKTWMGKFKD-KNVAKCAARMGLCFSSTYATVDVMPHEVDTEVPDIERN-----GYV 609
Query: 575 FSDGIGKISASFARKVAQ--KCGIRHTPSAFQIRYAGFKGVISIDPTSS--VKLSLRNSM 634
FSDGIG I+ A +V + K + ++P A+QIRYAGFKGV++ P+ S ++L+LR+SM
Sbjct: 610 FSDGIGTITPDLADEVMEKLKLDVHYSPCAYQIRYAGFKGVVARWPSKSDGIRLALRDSM 669
Query: 635 LKYESADTKLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQKELIEQLDTIFTDPM 694
K+ S T L++ SW+++ P FLNRQ+ITLLS LGV D +F Q+ ++ +L+ I D
Sbjct: 670 KKFFSKHTILEICSWTRFQPGFLNRQIITLLSVLGVPDEIFWDMQESMLYKLNRILDDTD 729
Query: 695 KAQQALELMSPGENTNILKEMILCGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPNG 754
A + L S E N M+ G++P +EP L ML + R ++L LR KSRIF+ +G
Sbjct: 730 VAFEVL-TASCAEQGNTAAIMLSAGFKPKTEPHLRGMLSSVRIAQLWGLREKSRIFVTSG 789
Query: 755 RAMMGCLDETGNLDYGEVFVQCS--AHQKLHNDH-IIFRRSKSNRHFIVTGTVVVAKNPC 814
R +MGCLDE G L++G+ F+Q S + + + H F+ +K++ +V G V +AKNPC
Sbjct: 790 RWLMGCLDEAGILEHGQCFIQVSKPSIENCFSKHGSRFKETKTDLE-VVKGYVAIAKNPC 849
Query: 815 LHPGDVRVLTAVDVPSLHHMVDCVVFPQRGSRPHPNECSGSDLDGDIYFVCWDPDLIPP- 874
LHPGDVR+L AVDVP LHHM DC++FPQ+G RPH NE SGSDLDGD+YFV WD LIPP
Sbjct: 850 LHPGDVRILEAVDVPQLHHMYDCLIFPQKGDRPHTNEASGSDLDGDLYFVAWDQKLIPPN 909
Query: 875 -QQVEPMDYTPVPSKLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADREPEKAMSK 934
+ M Y K L V +++ ++FA + N+ LG I NAH ADR AM +
Sbjct: 910 RKSYPAMHYDAAEEKSLGRAVNHQDIIDFFARNLANEQLGTICNAHVVHADRSEYGAMDE 969
Query: 935 TCIKLAKLFSIAVDFPKTGVPALISADLRVQAYPDFMDKADKVSYKSDNVLGKLFRMLDN 994
C+ LA+L + AVDFPKTG + L+ + YPDFM K D +YKS+ +LG+L+R +
Sbjct: 970 ECLLLAELAATAVDFPKTGKIVSMPFHLKPKLYPDFMGKEDYQTYKSNKILGRLYRRVKE 1029
Query: 995 VGPKIKIRSLTFTREEAQQAYDPDMEVEGFEEYLDDAFYHKNNYDMRLGNLMNYYKIKTE 1054
V + S + + + YD +E+ GFE+ + +A+ HK YD +L L+ YK++ E
Sbjct: 1030 VYDEDAEASSEESTDPSAIPYDAVLEIPGFEDLIPEAWGHKCLYDGQLIGLLGQYKVQKE 1089
Query: 1055 AELISG--GSLTKSLSFTKKNEAESITMAVKSLRKEARGWFNENAELHFG---HDKNVY- 1107
E+++G S+ K S + E + + SL+KE R F E H +KN+
Sbjct: 1090 EEIVTGHIWSMPKYTSKKQGELKERLKHSYNSLKKEFRKVFEETIPDHENLSEEEKNILY 1149
BLAST of Spg028666 vs. ExPASy TrEMBL
Match:
A0A6J1FR02 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111447986 PE=3 SV=1)
HSP 1 Score: 2018.8 bits (5229), Expect = 0.0e+00
Identity = 980/1115 (87.89%), Postives = 1042/1115 (93.45%), Query Frame = 0
Query: 1 MGKTIQLYGFPPGVLQEAVKAFVERFTGEGTIDAIKIKRSKGRGRRVYATIQFTDDEGAK 60
MGKTIQLYGFP GVLQE+VKAFVERFTGEG+IDAI +KRS+GRG+R+YA IQF DD GAK
Sbjct: 1 MGKTIQLYGFPSGVLQESVKAFVERFTGEGSIDAINVKRSRGRGKRLYAIIQFIDDVGAK 60
Query: 61 LIISLANRRLCYDTSYLKAREWKQDILPDPLVFEYNFKGLRLHLGCQISKERFSVLWTES 120
LIISLAN L Y TSYLKAREWKQDIL DPLVFEYNFKGL LHLGCQIS+E F VLW ES
Sbjct: 61 LIISLANASLRYGTSYLKAREWKQDILQDPLVFEYNFKGLELHLGCQISREEFFVLWRES 120
Query: 121 TVSVDFGFERRELKFFISHPHVDYMLVLRYENIWQVELHKPQGQSVDYLLIQLFGAPRIY 180
VSV+F FERR+LKFFISHP VDYMLVLRYENIWQVELHKPQGQS+DYLLIQL GAPRIY
Sbjct: 121 NVSVNFEFERRDLKFFISHPRVDYMLVLRYENIWQVELHKPQGQSIDYLLIQLVGAPRIY 180
Query: 181 ERDTKSYRPITEDPFFTFSEEIDTKWFRATDFTPSCCIGQSAALCLEIPYGRQLPNFHDN 240
ERDTK + I+EDPF +FS+E+DTKWFRATDFTPSCCIGQS ALCLEIPYGR LPNFHD
Sbjct: 181 ERDTKLFGHISEDPFLSFSKEMDTKWFRATDFTPSCCIGQSGALCLEIPYGRLLPNFHDK 240
Query: 241 FAYYKEIKGKLTLVSGSTYSSNVNLVPVVRPPLNINLPYAILFKINLLVQQGCLPGPALD 300
FAY+ EIKGK TL SGSTYSSNVNLVPVVRPPL+I+LPYAILFKINLLVQQGCLPGP+LD
Sbjct: 241 FAYFNEIKGKFTLASGSTYSSNVNLVPVVRPPLSIHLPYAILFKINLLVQQGCLPGPSLD 300
Query: 301 ISFYQMVDPQIHNIAFIDHALKKLYHLKECCYNPSKWLDDEYRKYFRLKNPPLPPIFTLN 360
ISFY+MVDPQIHNIAFID AL+KLY LKECCYNPSKWL +EYRKY +L NPP PP +L+
Sbjct: 301 ISFYEMVDPQIHNIAFIDRALRKLYDLKECCYNPSKWLGEEYRKYSKLTNPPQPPNLSLD 360
Query: 361 EGLVYVHRVQVTPCKVYFCGPEVNVSNRVLRGYPKYLDNFLRVSFVDEELDKMYSTELSP 420
EGLVYVHRVQVTPC+VYFCGPEVNVSNRVLR YP+YLDNFLRVSFVDEELDK+YSTELSP
Sbjct: 361 EGLVYVHRVQVTPCRVYFCGPEVNVSNRVLRRYPEYLDNFLRVSFVDEELDKLYSTELSP 420
Query: 421 RESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDGLTAAK 480
RESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDG+TAAK
Sbjct: 421 RESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDGITAAK 480
Query: 481 IRKGMGDFHSIRNVAKYAARLGQSFGSSTETLSVSRREVEVIPDIEVESGSGVNYVFSDG 540
IRK MGDFHSIRNVAKYAARLGQSFGSSTET SVSRREV+VIPDIEV+S +G+NYVFSDG
Sbjct: 481 IRKWMGDFHSIRNVAKYAARLGQSFGSSTETASVSRREVKVIPDIEVKSDAGINYVFSDG 540
Query: 541 IGKISASFARKVAQKCGIRHTPSAFQIRYAGFKGVISIDPTSSVKLSLRNSMLKYESADT 600
IGKISASFAR+VA+KC IRHTPSAFQIRYAGFKGVIS+DPTS VKLSLRNSMLKYES DT
Sbjct: 541 IGKISASFAREVARKCHIRHTPSAFQIRYAGFKGVISVDPTSFVKLSLRNSMLKYESTDT 600
Query: 601 KLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQKELIEQLDTIFTDPMKAQQALEL 660
KLDVLSWS+YHPC+LNRQLITLLSTLGVQD +FESKQKELI+QLDTIF DP+KAQQALEL
Sbjct: 601 KLDVLSWSRYHPCYLNRQLITLLSTLGVQDDIFESKQKELIDQLDTIFMDPLKAQQALEL 660
Query: 661 MSPGENTNILKEMILCGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPNGRAMMGCLD 720
MSPGENTNILKE+I CGYRPDSEPFL MMLHTFRESKLLELRRKSRIFIPNGRAMMGCLD
Sbjct: 661 MSPGENTNILKEIISCGYRPDSEPFLYMMLHTFRESKLLELRRKSRIFIPNGRAMMGCLD 720
Query: 721 ETGNLDYGEVFVQCSAHQKLHNDHIIFRRSKSNRHFIVTGTVVVAKNPCLHPGDVRVLTA 780
ETGNL+YGEVFVQCSAHQ+LHNDHIIF+RSKSN+HFIVTGTVVVAKNPCLHPGDVRVLTA
Sbjct: 721 ETGNLEYGEVFVQCSAHQQLHNDHIIFKRSKSNQHFIVTGTVVVAKNPCLHPGDVRVLTA 780
Query: 781 VDVPSLHHMVDCVVFPQRGSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP 840
VDVPSLHHMVDC+VFPQ+G RPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP
Sbjct: 781 VDVPSLHHMVDCLVFPQKGPRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP 840
Query: 841 SKLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADREPEKAMSKTCIKLAKLFSIAV 900
S+LLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFAD PEKAMS+ CIKLAKLFSIAV
Sbjct: 841 SRLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADSRPEKAMSEPCIKLAKLFSIAV 900
Query: 901 DFPKTGVPALISADLRVQAYPDFMDKADKVSYKSDNVLGKLFRMLDNVGPKIKIRSLTFT 960
DFPKTGVPALISADLRVQ YPDFMDK DK SYKS NVLGKLFRMLD++ P+I I+S+ FT
Sbjct: 901 DFPKTGVPALISADLRVQVYPDFMDKPDKQSYKSHNVLGKLFRMLDDIEPRISIKSVKFT 960
Query: 961 REEAQQAYDPDMEVEGFEEYLDDAFYHKNNYDMRLGNLMNYYKIKTEAELISGGSLTKSL 1020
RE A+QAYDPDMEV+GFEEYLDDA YHKNNYDMRLGNLM YYKIKTEAELISG SLTKS
Sbjct: 961 REIARQAYDPDMEVKGFEEYLDDALYHKNNYDMRLGNLMKYYKIKTEAELISGVSLTKSS 1020
Query: 1021 SFTKKNEAESITMAVKSLRKEARGWFNENAELHFGHDKNVYARASAWYFVTYHHSYWGWY 1080
SFTKKNEAESI MAVKSLRKEARGWFNENA LHFGHD N YARASAWYFVTYH+SYWGWY
Sbjct: 1021 SFTKKNEAESIIMAVKSLRKEARGWFNENAGLHFGHDNNAYARASAWYFVTYHYSYWGWY 1080
Query: 1081 DDGKKHGHFLSFPWCVHDKLIRIKKRKMNARARYR 1116
+ K HFLSFPWCV+DKLIRIKKRK+ ARAR+R
Sbjct: 1081 GE-KNESHFLSFPWCVYDKLIRIKKRKIGARARHR 1114
BLAST of Spg028666 vs. ExPASy TrEMBL
Match:
E9NWK7 (RNA-dependent RNA polymerase OS=Cucumis sativus OX=3659 GN=Csa_5G239640 PE=2 SV=1)
HSP 1 Score: 2007.6 bits (5200), Expect = 0.0e+00
Identity = 974/1115 (87.35%), Postives = 1035/1115 (92.83%), Query Frame = 0
Query: 1 MGKTIQLYGFPPGVLQEAVKAFVERFTGEGTIDAIKIKRSKGRGRRVYATIQFTDDEGAK 60
MGKTIQL+GFP GVLQE+VK FVE TG GTIDAI KRSKG GRRVYA IQFTD+EGAK
Sbjct: 1 MGKTIQLFGFPSGVLQESVKTFVEGITGTGTIDAINTKRSKGGGRRVYAIIQFTDEEGAK 60
Query: 61 LIISLANRRLCYDTSYLKAREWKQDILPDPLVFEYNFKGLRLHLGCQISKERFSVLWTES 120
IIS A RLCY TSYLKARE K DILPDPLVF+YNFK LRLHLGCQISKE FSVLWTES
Sbjct: 61 SIISKATERLCYGTSYLKAREMKHDILPDPLVFDYNFKALRLHLGCQISKESFSVLWTES 120
Query: 121 TVSVDFGFERRELKFFISHPHVDYMLVLRYENIWQVELHKPQGQSVDYLLIQLFGAPRIY 180
VSVDFGFE R+L FFIS+P VDYMLVLRYENIWQVELHKP GQSVDYLLIQLFGAPRIY
Sbjct: 121 NVSVDFGFELRKLYFFISYPRVDYMLVLRYENIWQVELHKPHGQSVDYLLIQLFGAPRIY 180
Query: 181 ERDTKSYRPITEDPFFTFSEEIDTKWFRATDFTPSCCIGQSAALCLEIPYGRQLPNFHDN 240
ERD +S+ ITEDPF FS EIDT+WFRATDFTPSC IGQSAALCLEIPYGRQLPNFHD
Sbjct: 181 ERDARSFGLITEDPFLNFSTEIDTQWFRATDFTPSCSIGQSAALCLEIPYGRQLPNFHDK 240
Query: 241 FAYYKEIKGKLTLVSGSTYSSNVNLVPVVRPPLNINLPYAILFKINLLVQQGCLPGPALD 300
FAY+KEIKGK TLVSGSTYSSNVNLVPVV PP INLPY ILFKINLLVQQGCLPGPALD
Sbjct: 241 FAYFKEIKGKFTLVSGSTYSSNVNLVPVVTPPRTINLPYTILFKINLLVQQGCLPGPALD 300
Query: 301 ISFYQMVDPQIHNIAFIDHALKKLYHLKECCYNPSKWLDDEYRKYFRLKNPPLPPIFTLN 360
ISFYQMVD QI+N A IDHALKKL HLKECCYNPSKWLD+EYRKYF+LKNPP PPI TLN
Sbjct: 301 ISFYQMVDSQIYNTAVIDHALKKLLHLKECCYNPSKWLDEEYRKYFKLKNPPQPPILTLN 360
Query: 361 EGLVYVHRVQVTPCKVYFCGPEVNVSNRVLRGYPKYLDNFLRVSFVDEELDKMYSTELSP 420
EGLVYVHRVQVTPCKVYFCGPEVN+SNRVLR YP Y+DNFLRVSFVDEEL KMYSTELSP
Sbjct: 361 EGLVYVHRVQVTPCKVYFCGPEVNISNRVLRRYPDYIDNFLRVSFVDEELGKMYSTELSP 420
Query: 421 RESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDGLTAAK 480
R SSSLEDGKTKIFKRILSVL+DGITIGDKKFEFLAYSSSQLRENA WMFAP++ LTAAK
Sbjct: 421 RASSSLEDGKTKIFKRILSVLRDGITIGDKKFEFLAYSSSQLRENAAWMFAPKNELTAAK 480
Query: 481 IRKGMGDFHSIRNVAKYAARLGQSFGSSTETLSVSRREVEVIPDIEVESGSGVNYVFSDG 540
IR+ MGDFH+IRNVAKYAARLGQSFGSSTETLSVSRREV+VIPDIEVESGSGVNYVFSDG
Sbjct: 481 IRQWMGDFHNIRNVAKYAARLGQSFGSSTETLSVSRREVKVIPDIEVESGSGVNYVFSDG 540
Query: 541 IGKISASFARKVAQKCGIRHTPSAFQIRYAGFKGVISIDPTSSVKLSLRNSMLKYESADT 600
IGKI+ASFARKVA+KCGIRHTPSAFQIRYAGFKGVIS+DPTSSVKLSLRNSMLKYES DT
Sbjct: 541 IGKIAASFARKVAKKCGIRHTPSAFQIRYAGFKGVISVDPTSSVKLSLRNSMLKYESTDT 600
Query: 601 KLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQKELIEQLDTIFTDPMKAQQALEL 660
KLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQ+ELI++LDTIF+DP+KAQQALEL
Sbjct: 601 KLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQQELIDELDTIFSDPLKAQQALEL 660
Query: 661 MSPGENTNILKEMILCGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPNGRAMMGCLD 720
MSPGENT ILKEM+LCGY+PDSEPFL MMLHTFRESKL+ELR KSRIFIPNGRAMMGCLD
Sbjct: 661 MSPGENTKILKEMMLCGYKPDSEPFLRMMLHTFRESKLMELRMKSRIFIPNGRAMMGCLD 720
Query: 721 ETGNLDYGEVFVQCSAHQKLHNDHIIFRRSKSNRHFIVTGTVVVAKNPCLHPGDVRVLTA 780
ET NL+YGEVFVQCSAHQ+LH+D +IF+R KSNRHFIVTGTVVVAKNPCLHPGDVRVLTA
Sbjct: 721 ETRNLEYGEVFVQCSAHQQLHDDRVIFKRIKSNRHFIVTGTVVVAKNPCLHPGDVRVLTA 780
Query: 781 VDVPSLHHMVDCVVFPQRGSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP 840
VDVPSLHHM+DCVVFPQ+GSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP
Sbjct: 781 VDVPSLHHMIDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP 840
Query: 841 SKLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADREPEKAMSKTCIKLAKLFSIAV 900
S++LDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFAD+EP+KAMS CI+LAKLFSIAV
Sbjct: 841 SQVLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADKEPKKAMSNPCIQLAKLFSIAV 900
Query: 901 DFPKTGVPALISADLRVQAYPDFMDKADKVSYKSDNVLGKLFRMLDNVGPKIK-IRSLTF 960
DFPKTGVPALI A+LRV+ YPDFMDKADKV+Y+S+NVLGKLFRMLD++GP IK IRS +
Sbjct: 901 DFPKTGVPALIPANLRVKEYPDFMDKADKVTYESENVLGKLFRMLDSIGPNIKNIRSFNY 960
Query: 961 TREEAQQAYDPDMEVEGFEEYLDDAFYHKNNYDMRLGNLMNYYKIKTEAELISGGSLTKS 1020
T E A+Q YDPDMEVEGFEEYLDDA YHKNNYDMRLGNLM+Y+KIKTEAELISGGSLT S
Sbjct: 961 TPEMARQDYDPDMEVEGFEEYLDDAIYHKNNYDMRLGNLMHYHKIKTEAELISGGSLTSS 1020
Query: 1021 LSFTKKNEAESITMAVKSLRKEARGWFNENAELHFGHDKNVYARASAWYFVTYHHSYWGW 1080
LSFT KNEAESI +AVKSLRKEARGWFNE A+LH+GH NVYARASAWYFVTYHH+YWGW
Sbjct: 1021 LSFTMKNEAESIILAVKSLRKEARGWFNEKADLHYGHHTNVYARASAWYFVTYHHTYWGW 1080
Query: 1081 YDDGKKHGHFLSFPWCVHDKLIRIKKRKMNARARY 1115
D K HGHFLSFPWCV+DKLIRIK RK+N RARY
Sbjct: 1081 SDGRKNHGHFLSFPWCVYDKLIRIKHRKINCRARY 1115
BLAST of Spg028666 vs. ExPASy TrEMBL
Match:
A0A6J1K2Q9 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111490531 PE=3 SV=1)
HSP 1 Score: 1998.8 bits (5177), Expect = 0.0e+00
Identity = 973/1115 (87.26%), Postives = 1037/1115 (93.00%), Query Frame = 0
Query: 1 MGKTIQLYGFPPGVLQEAVKAFVERFTGEGTIDAIKIKRSKGRGRRVYATIQFTDDEGAK 60
MGKTIQLYGFP GVLQE+VKAFVERFTGEG+IDAI +KRS+GRG+R+YA IQF DD GAK
Sbjct: 1 MGKTIQLYGFPSGVLQESVKAFVERFTGEGSIDAINVKRSRGRGKRLYAIIQFIDDVGAK 60
Query: 61 LIISLANRRLCYDTSYLKAREWKQDILPDPLVFEYNFKGLRLHLGCQISKERFSVLWTES 120
LIISLAN L Y TS LKAREWKQDIL DPLVFEYNFKGL LHLGCQIS+E F VLWTES
Sbjct: 61 LIISLANASLRYGTSSLKAREWKQDILQDPLVFEYNFKGLELHLGCQISREEFFVLWTES 120
Query: 121 TVSVDFGFERRELKFFISHPHVDYMLVLRYENIWQVELHKPQGQSVDYLLIQLFGAPRIY 180
VSV+F FERR+LKFFISHP VDYMLVLRYENIWQVELHKP GQS+DYLLIQL GAPRIY
Sbjct: 121 NVSVNFEFERRDLKFFISHPLVDYMLVLRYENIWQVELHKPPGQSIDYLLIQLVGAPRIY 180
Query: 181 ERDTKSYRPITEDPFFTFSEEIDTKWFRATDFTPSCCIGQSAALCLEIPYGRQLPNFHDN 240
ERDTK + I+EDPF +FS+E+DT+WFRATDFTPSCCIGQS ALCLEIPYGRQLPNFHD
Sbjct: 181 ERDTKLFGHISEDPFLSFSKEMDTEWFRATDFTPSCCIGQSGALCLEIPYGRQLPNFHDK 240
Query: 241 FAYYKEIKGKLTLVSGSTYSSNVNLVPVVRPPLNINLPYAILFKINLLVQQGCLPGPALD 300
FAY+KEIKGK TL +GSTYSSNVNLVPVVRPPL+I+LPYAILFKINLLVQQGCLPGPALD
Sbjct: 241 FAYFKEIKGKFTLATGSTYSSNVNLVPVVRPPLSIHLPYAILFKINLLVQQGCLPGPALD 300
Query: 301 ISFYQMVDPQIHNIAFIDHALKKLYHLKECCYNPSKWLDDEYRKYFRLKNPPLPPIFTLN 360
ISFY+MVDPQIHNIAFID AL+KLY LKECCYNPSKWL +EYRKY +L NPP PP +L+
Sbjct: 301 ISFYEMVDPQIHNIAFIDRALRKLYDLKECCYNPSKWLGEEYRKYSKLTNPPQPPNLSLD 360
Query: 361 EGLVYVHRVQVTPCKVYFCGPEVNVSNRVLRGYPKYLDNFLRVSFVDEELDKMYSTELSP 420
EGLVYVHRVQVTPC+VYFCGPEVNVSNRVLR YP+YLDNFLRVSFVDEELD++YSTELSP
Sbjct: 361 EGLVYVHRVQVTPCRVYFCGPEVNVSNRVLRRYPEYLDNFLRVSFVDEELDRLYSTELSP 420
Query: 421 RESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDGLTAAK 480
RESSSL DGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDG+TAAK
Sbjct: 421 RESSSLGDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDGITAAK 480
Query: 481 IRKGMGDFHSIRNVAKYAARLGQSFGSSTETLSVSRREVEVIPDIEVESGSGVNYVFSDG 540
IRK MGDFHSIRNVAKYAARLGQSFGSSTET SVSRREVEVIPDIEVES +G+NYVFSDG
Sbjct: 481 IRKWMGDFHSIRNVAKYAARLGQSFGSSTETASVSRREVEVIPDIEVESDAGINYVFSDG 540
Query: 541 IGKISASFARKVAQKCGIRHTPSAFQIRYAGFKGVISIDPTSSVKLSLRNSMLKYESADT 600
IGKISASFAR+VA+KC IRHTPSAFQIRYAGFKGVIS+DPTS VKLSLRNSMLKYES D
Sbjct: 541 IGKISASFAREVARKCRIRHTPSAFQIRYAGFKGVISVDPTSFVKLSLRNSMLKYESTDM 600
Query: 601 KLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQKELIEQLDTIFTDPMKAQQALEL 660
KLDVLSWS+YHPC+LNRQLITLLSTLGVQD +FESKQKELI+QLDTIF +P+KAQQALEL
Sbjct: 601 KLDVLSWSRYHPCYLNRQLITLLSTLGVQDDIFESKQKELIDQLDTIFMNPLKAQQALEL 660
Query: 661 MSPGENTNILKEMILCGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPNGRAMMGCLD 720
MSPGENTNILKE+I CGYRPDSEPFL MMLHTFRESKLLELRRKSRIFIPNGRAMMGCLD
Sbjct: 661 MSPGENTNILKEIISCGYRPDSEPFLYMMLHTFRESKLLELRRKSRIFIPNGRAMMGCLD 720
Query: 721 ETGNLDYGEVFVQCSAHQKLHNDHIIFRRSKSNRHFIVTGTVVVAKNPCLHPGDVRVLTA 780
ETGNL+YGEVFVQCSAHQ+LHNDHIIF+RSKSN+HFIVTGTVVVAKNPCLHPGDVRVLTA
Sbjct: 721 ETGNLEYGEVFVQCSAHQQLHNDHIIFKRSKSNQHFIVTGTVVVAKNPCLHPGDVRVLTA 780
Query: 781 VDVPSLHHMVDCVVFPQRGSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP 840
VDVP+LHHMVDC+VFPQ+G RPHPNECSGSDLDGDIYFVCWDPDLIP QQVEPMDYTPV
Sbjct: 781 VDVPALHHMVDCLVFPQKGPRPHPNECSGSDLDGDIYFVCWDPDLIPHQQVEPMDYTPVL 840
Query: 841 SKLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADREPEKAMSKTCIKLAKLFSIAV 900
S+LLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFAD PEKAMS+ CIKLAKLFSIAV
Sbjct: 841 SRLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADSWPEKAMSEPCIKLAKLFSIAV 900
Query: 901 DFPKTGVPALISADLRVQAYPDFMDKADKVSYKSDNVLGKLFRMLDNVGPKIKIRSLTFT 960
DFPKTGVPALISADLRVQ YPDFMDK DK YKS NVLGKLFRMLD++ P+I I+S+ FT
Sbjct: 901 DFPKTGVPALISADLRVQVYPDFMDKPDKQRYKSHNVLGKLFRMLDDIEPRISIKSVKFT 960
Query: 961 REEAQQAYDPDMEVEGFEEYLDDAFYHKNNYDMRLGNLMNYYKIKTEAELISGGSLTKSL 1020
RE A+QAYDPDMEVEGFEEYLDDA YHKNNYDMRLGNLM YYKIKTEAELISG SLTKS
Sbjct: 961 REIARQAYDPDMEVEGFEEYLDDALYHKNNYDMRLGNLMKYYKIKTEAELISGVSLTKSS 1020
Query: 1021 SFTKKNEAESITMAVKSLRKEARGWFNENAELHFGHDKNVYARASAWYFVTYHHSYWGWY 1080
SFTKKNEAESI MAVKSLRKEARGWFNENA LHFGHD N YARASAWYFVTYH+SYWGW+
Sbjct: 1021 SFTKKNEAESIIMAVKSLRKEARGWFNENAGLHFGHDNNAYARASAWYFVTYHYSYWGWF 1080
Query: 1081 DDGKKHGHFLSFPWCVHDKLIRIKKRKMNARARYR 1116
+ K HFLSFPWCV+DKLIRIKKRK+ RAR+R
Sbjct: 1081 GE-KNESHFLSFPWCVYDKLIRIKKRKIGPRARHR 1114
BLAST of Spg028666 vs. ExPASy TrEMBL
Match:
H3K3Y9 (RNA-dependent RNA polymerase OS=Cucumis sativus OX=3659 GN=RDR1 PE=2 SV=1)
HSP 1 Score: 1995.3 bits (5168), Expect = 0.0e+00
Identity = 969/1115 (86.91%), Postives = 1031/1115 (92.47%), Query Frame = 0
Query: 1 MGKTIQLYGFPPGVLQEAVKAFVERFTGEGTIDAIKIKRSKGRGRRVYATIQFTDDEGAK 60
MGKTIQL+GFP GVLQE+VK FVE TG GTIDAI KRSKG GRRVYA IQFTD+EGAK
Sbjct: 1 MGKTIQLFGFPSGVLQESVKTFVEGITGTGTIDAINTKRSKGGGRRVYAIIQFTDEEGAK 60
Query: 61 LIISLANRRLCYDTSYLKAREWKQDILPDPLVFEYNFKGLRLHLGCQISKERFSVLWTES 120
IIS A RLCY TSYLKARE K DILPDPLVF+YNFK LRLHLGCQISKE FSVLWTES
Sbjct: 61 SIISKATERLCYGTSYLKAREMKHDILPDPLVFDYNFKALRLHLGCQISKESFSVLWTES 120
Query: 121 TVSVDFGFERRELKFFISHPHVDYMLVLRYENIWQVELHKPQGQSVDYLLIQLFGAPRIY 180
VSVDFGF+ R+L FFIS+P VDYMLVLRYENIWQVELHKP GQSVDYLLIQLFGAPRIY
Sbjct: 121 NVSVDFGFDVRKLYFFISYPRVDYMLVLRYENIWQVELHKPHGQSVDYLLIQLFGAPRIY 180
Query: 181 ERDTKSYRPITEDPFFTFSEEIDTKWFRATDFTPSCCIGQSAALCLEIPYGRQLPNFHDN 240
ERD +S+ ITEDPF FS EIDT+WFRATDFTPSC IGQSAALCLEI YGRQLPNFHD
Sbjct: 181 ERDARSFGLITEDPFLNFSTEIDTQWFRATDFTPSCSIGQSAALCLEILYGRQLPNFHDK 240
Query: 241 FAYYKEIKGKLTLVSGSTYSSNVNLVPVVRPPLNINLPYAILFKINLLVQQGCLPGPALD 300
FAY+KEIKGK TLVSGSTYSSNVNLVPVV PP INLPY ILFKINLLVQQGCLPGPALD
Sbjct: 241 FAYFKEIKGKFTLVSGSTYSSNVNLVPVVTPPRTINLPYTILFKINLLVQQGCLPGPALD 300
Query: 301 ISFYQMVDPQIHNIAFIDHALKKLYHLKECCYNPSKWLDDEYRKYFRLKNPPLPPIFTLN 360
ISFYQMVD QI+N A IDHALKKL HLKECCYNPSKWLD+EYRKYF+LKNPP PPI TLN
Sbjct: 301 ISFYQMVDSQIYNTAVIDHALKKLLHLKECCYNPSKWLDEEYRKYFKLKNPPQPPILTLN 360
Query: 361 EGLVYVHRVQVTPCKVYFCGPEVNVSNRVLRGYPKYLDNFLRVSFVDEELDKMYSTELSP 420
EGLVYVHRVQVTPCKVYFCGPEVN+SNRVLR YP Y+DNFLRVSFVDEEL KMYSTELSP
Sbjct: 361 EGLVYVHRVQVTPCKVYFCGPEVNISNRVLRRYPDYIDNFLRVSFVDEELGKMYSTELSP 420
Query: 421 RESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDGLTAAK 480
R SSSLEDGKTKI KRILSVL+DGITIGDKKFEFLAYSSSQLRENA WMFAP++ LTAAK
Sbjct: 421 RASSSLEDGKTKILKRILSVLRDGITIGDKKFEFLAYSSSQLRENAAWMFAPKNELTAAK 480
Query: 481 IRKGMGDFHSIRNVAKYAARLGQSFGSSTETLSVSRREVEVIPDIEVESGSGVNYVFSDG 540
IR+ MGDFH+IRNVAKYAARLGQ FGSSTETLSVSRREV+VIPDIEVESGSGVNYVFSDG
Sbjct: 481 IRQWMGDFHNIRNVAKYAARLGQPFGSSTETLSVSRREVKVIPDIEVESGSGVNYVFSDG 540
Query: 541 IGKISASFARKVAQKCGIRHTPSAFQIRYAGFKGVISIDPTSSVKLSLRNSMLKYESADT 600
IGKI+ASFARKVA+KCGIRHTPSAFQIRYAGFKGVIS+DPTSSVKLSLRNSMLKYES DT
Sbjct: 541 IGKIAASFARKVAKKCGIRHTPSAFQIRYAGFKGVISVDPTSSVKLSLRNSMLKYESTDT 600
Query: 601 KLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQKELIEQLDTIFTDPMKAQQALEL 660
KLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQ+ELI++LDTIF+DP+KAQQALEL
Sbjct: 601 KLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQQELIDELDTIFSDPLKAQQALEL 660
Query: 661 MSPGENTNILKEMILCGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPNGRAMMGCLD 720
MSPGENT ILKEM+LCGY+PDSEPFL MMLHTFRESKL+ELR KSRIFIPNGRAMMGCLD
Sbjct: 661 MSPGENTKILKEMMLCGYKPDSEPFLRMMLHTFRESKLMELRMKSRIFIPNGRAMMGCLD 720
Query: 721 ETGNLDYGEVFVQCSAHQKLHNDHIIFRRSKSNRHFIVTGTVVVAKNPCLHPGDVRVLTA 780
ET NL+YGEVF QCSAHQ+LH+D +IF+R KSNRHFIVTGTVVVAKNPCLHPGDVRVLTA
Sbjct: 721 ETRNLEYGEVFWQCSAHQQLHDDRVIFKRIKSNRHFIVTGTVVVAKNPCLHPGDVRVLTA 780
Query: 781 VDVPSLHHMVDCVVFPQRGSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP 840
VDVPSLHHM+DCVVFPQ+GSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP
Sbjct: 781 VDVPSLHHMIDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP 840
Query: 841 SKLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADREPEKAMSKTCIKLAKLFSIAV 900
S++LDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFAD+EP+KAMS CI+LAKLFSIAV
Sbjct: 841 SQVLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADKEPKKAMSNPCIQLAKLFSIAV 900
Query: 901 DFPKTGVPALISADLRVQAYPDFMDKADKVSYKSDNVLGKLFRMLDNVGPKIK-IRSLTF 960
DFPKTGVPALI A+LRV+ YPDFMDKADKV+Y+S+NVLGKLFRMLD++GP IK IRS +
Sbjct: 901 DFPKTGVPALIPANLRVKEYPDFMDKADKVTYESENVLGKLFRMLDSIGPNIKNIRSFNY 960
Query: 961 TREEAQQAYDPDMEVEGFEEYLDDAFYHKNNYDMRLGNLMNYYKIKTEAELISGGSLTKS 1020
T E A+Q YDPDMEVEGFEEYLDDA YHKNNYDMRLGNLM+Y+KIKTEAELISGGSLT S
Sbjct: 961 TPEMARQDYDPDMEVEGFEEYLDDAIYHKNNYDMRLGNLMHYHKIKTEAELISGGSLTSS 1020
Query: 1021 LSFTKKNEAESITMAVKSLRKEARGWFNENAELHFGHDKNVYARASAWYFVTYHHSYWGW 1080
LSFT KNEAESI +AVKSLRKEARGWFNE A+LH+GH NVYARASAWYFVTYHH+YWGW
Sbjct: 1021 LSFTMKNEAESIILAVKSLRKEARGWFNEKADLHYGHHTNVYARASAWYFVTYHHTYWGW 1080
Query: 1081 YDDGKKHGHFLSFPWCVHDKLIRIKKRKMNARARY 1115
D K HGHFLSFPWCV+DKLIRIK RK+N RARY
Sbjct: 1081 SDGRKNHGHFLSFPWCVYDKLIRIKHRKINCRARY 1115
BLAST of Spg028666 vs. ExPASy TrEMBL
Match:
A0A0S1YCZ7 (RNA-dependent RNA polymerase OS=Cucumis sativus OX=3659 GN=RDR1b PE=2 SV=1)
HSP 1 Score: 1994.2 bits (5165), Expect = 0.0e+00
Identity = 970/1115 (87.00%), Postives = 1031/1115 (92.47%), Query Frame = 0
Query: 1 MGKTIQLYGFPPGVLQEAVKAFVERFTGEGTIDAIKIKRSKGRGRRVYATIQFTDDEGAK 60
MGKTIQL+GFP GVLQE+VK FVE TG GTIDAI KRSKG GRRVYA IQFTD+EGAK
Sbjct: 1 MGKTIQLFGFPSGVLQESVKTFVEGITGTGTIDAINTKRSKGGGRRVYAIIQFTDEEGAK 60
Query: 61 LIISLANRRLCYDTSYLKAREWKQDILPDPLVFEYNFKGLRLHLGCQISKERFSVLWTES 120
IIS A RLCY TSYLKARE K DILPDPLVF+YNFK LRLHLGCQISKE FSVLWTES
Sbjct: 61 SIISKATERLCYGTSYLKAREMKHDILPDPLVFDYNFKALRLHLGCQISKESFSVLWTES 120
Query: 121 TVSVDFGFERRELKFFISHPHVDYMLVLRYENIWQVELHKPQGQSVDYLLIQLFGAPRIY 180
VSVDFGFE R+L FFIS+P VDYMLVLRYENIWQVELHKP GQSVDYLLIQLFGAPRIY
Sbjct: 121 NVSVDFGFELRKLYFFISYPRVDYMLVLRYENIWQVELHKPHGQSVDYLLIQLFGAPRIY 180
Query: 181 ERDTKSYRPITEDPFFTFSEEIDTKWFRATDFTPSCCIGQSAALCLEIPYGRQLPNFHDN 240
ERD +S+ ITEDPF FS EIDT+WFRATDFTPSC IGQSAALCLEIPYGRQLPNFHD
Sbjct: 181 ERDARSFGLITEDPFLNFSTEIDTQWFRATDFTPSCSIGQSAALCLEIPYGRQLPNFHDK 240
Query: 241 FAYYKEIKGKLTLVSGSTYSSNVNLVPVVRPPLNINLPYAILFKINLLVQQGCLPGPALD 300
FAY+KEIKGK TLVSGSTYSSNVNLVPVV PP INLPY ILFKINLLVQQGCLPGPALD
Sbjct: 241 FAYFKEIKGKFTLVSGSTYSSNVNLVPVVTPPRTINLPYTILFKINLLVQQGCLPGPALD 300
Query: 301 ISFYQMVDPQIHNIAFIDHALKKLYHLKECCYNPSKWLDDEYRKYFRLKNPPLPPIFTLN 360
ISFYQMVD QI+N A IDHALKKL HLKECCYNPS WLD+EYRKYF+LKNPP PPI TLN
Sbjct: 301 ISFYQMVDSQIYNTAVIDHALKKLLHLKECCYNPSIWLDEEYRKYFKLKNPPQPPILTLN 360
Query: 361 EGLVYVHRVQVTPCKVYFCGPEVNVSNRVLRGYPKYLDNFLRVSFVDEELDKMYSTELSP 420
EGLVYVHRVQVTPCKVYFCGPEVN+SNRVLR P Y+DNFLRVSFVDEEL KMYSTELSP
Sbjct: 361 EGLVYVHRVQVTPCKVYFCGPEVNISNRVLRRCPDYIDNFLRVSFVDEELGKMYSTELSP 420
Query: 421 RESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDGLTAAK 480
R SSSLEDGKTKIFKRILSVL+DGITIGDKKFEFLAYSSSQLRENA WMFAP++ LTAAK
Sbjct: 421 RASSSLEDGKTKIFKRILSVLRDGITIGDKKFEFLAYSSSQLRENAAWMFAPKNELTAAK 480
Query: 481 IRKGMGDFHSIRNVAKYAARLGQSFGSSTETLSVSRREVEVIPDIEVESGSGVNYVFSDG 540
IR+ MGDFH+IRNVAKYAARLGQSFGSSTETLSVSRREV+VIPDIEVESGSGVNYVFSDG
Sbjct: 481 IRQWMGDFHNIRNVAKYAARLGQSFGSSTETLSVSRREVKVIPDIEVESGSGVNYVFSDG 540
Query: 541 IGKISASFARKVAQKCGIRHTPSAFQIRYAGFKGVISIDPTSSVKLSLRNSMLKYESADT 600
IGKI+ASFARKVA+KCGIRHTPSAFQIRYAGFKGVIS+DPTSSVKLSLRNSMLKYES DT
Sbjct: 541 IGKIAASFARKVAKKCGIRHTPSAFQIRYAGFKGVISVDPTSSVKLSLRNSMLKYESTDT 600
Query: 601 KLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQKELIEQLDTIFTDPMKAQQALEL 660
KLDVLSWSKYHPCFLNRQLIT LSTLGVQDHVFESKQ+ELI++LDTIF+DP+KAQQALEL
Sbjct: 601 KLDVLSWSKYHPCFLNRQLITFLSTLGVQDHVFESKQQELIDELDTIFSDPLKAQQALEL 660
Query: 661 MSPGENTNILKEMILCGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPNGRAMMGCLD 720
MSPGENT ILKEM+LCGY+PDSEPFL MMLHTFRESKL+ELR KSRIFIPNGRAMMGCLD
Sbjct: 661 MSPGENTKILKEMMLCGYKPDSEPFLRMMLHTFRESKLMELRMKSRIFIPNGRAMMGCLD 720
Query: 721 ETGNLDYGEVFVQCSAHQKLHNDHIIFRRSKSNRHFIVTGTVVVAKNPCLHPGDVRVLTA 780
ET NL+YGEVFVQCSAHQ+LH+D +IF+R KSNRHFIVTGTVVVAKNPCLHPGDVRVLTA
Sbjct: 721 ETRNLEYGEVFVQCSAHQQLHDDRVIFKRIKSNRHFIVTGTVVVAKNPCLHPGDVRVLTA 780
Query: 781 VDVPSLHHMVDCVVFPQRGSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP 840
VDVPSLHHM+DCVVFPQ+GSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP
Sbjct: 781 VDVPSLHHMIDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYTPVP 840
Query: 841 SKLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADREPEKAMSKTCIKLAKLFSIAV 900
S++LDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFAD+EP+KAMS CI+LAKLFSIAV
Sbjct: 841 SQVLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADKEPKKAMSNPCIQLAKLFSIAV 900
Query: 901 DFPKTGVPALISADLRVQAYPDFMDKADKVSYKSDNVLGKLFRMLDNVGPKIK-IRSLTF 960
DFPKTGVPALI A+LRV+ YPDFMDKADKV+Y+S+NVLGKLFRMLD++GP IK IRS +
Sbjct: 901 DFPKTGVPALIPANLRVKEYPDFMDKADKVTYESENVLGKLFRMLDSIGPNIKNIRSFNY 960
Query: 961 TREEAQQAYDPDMEVEGFEEYLDDAFYHKNNYDMRLGNLMNYYKIKTEAELISGGSLTKS 1020
T E A+Q YDPDMEVEGFEEYLDDA YHKNNYDMRLGNLM+Y+KIKTEAELISGGSLT S
Sbjct: 961 TPEMARQDYDPDMEVEGFEEYLDDAIYHKNNYDMRLGNLMHYHKIKTEAELISGGSLTSS 1020
Query: 1021 LSFTKKNEAESITMAVKSLRKEARGWFNENAELHFGHDKNVYARASAWYFVTYHHSYWGW 1080
LSFT KNEAESI +AVKSLRKEARGWFNE A+LH+GH NVYARASAWYFVTYHH+YWGW
Sbjct: 1021 LSFTMKNEAESIILAVKSLRKEARGWFNEKADLHYGHHTNVYARASAWYFVTYHHTYWGW 1080
Query: 1081 YDDGKKHGHFLSFPWCVHDKLIRIKKRKMNARARY 1115
D K HGHFLSFP CV+DKLIRIK RK+N RARY
Sbjct: 1081 SDGRKNHGHFLSFPRCVYDKLIRIKHRKINCRARY 1115
BLAST of Spg028666 vs. TAIR 10
Match:
AT1G14790.1 (RNA-dependent RNA polymerase 1 )
HSP 1 Score: 1355.5 bits (3507), Expect = 0.0e+00
Identity = 680/1117 (60.88%), Postives = 841/1117 (75.29%), Query Frame = 0
Query: 1 MGKTIQLYGFPPGVLQEAVKAFVERFTGEGTIDAIKIKRSKGRGRRVYATIQFTDDEGAK 60
MGKTIQ++GFP GV E VK F+ER TG GT+ AIK+++ K G RVYA +QFT + +
Sbjct: 1 MGKTIQVFGFPNGVSAEEVKKFLERLTGSGTVYAIKVRQPKKGGPRVYAIVQFTSERHTR 60
Query: 61 LIISLANRRLCYDTSYLKAREWKQDILPDPLVFEYNFKGLRLHLGCQISKERFSVLWTES 120
LII+ A RL Y SYLKA E +QDI+P P + GL++ GCQ+S ++F LW+
Sbjct: 61 LIITAAAERLYYGRSYLKAFEVEQDIVPKPRASLHTISGLKMFFGCQVSTKKFLTLWSAQ 120
Query: 121 TVSVDFGFERRELKFFISHPHVDYMLVLRYENIWQVELHKPQGQSVDYLLIQLFGAPRIY 180
V V FG R+L F S DY L L YENIWQ++LH PQG+S +L+IQ+ GAP+I+
Sbjct: 121 DVCVSFGIGMRKLHFSFSWYQKDYRLELSYENIWQIDLHSPQGRSSKFLVIQVIGAPKIF 180
Query: 181 ERDTKSYRPITEDPFFTFSEEIDTKWFRATDFTPSCCIGQSAALCLEIPYGRQLPNFHDN 240
E++ + + F +S+ D +W R TDFT S CIGQS A CLE+P +P+F +N
Sbjct: 181 EKEDQPINLLFGIMDF-YSDGSDEQWIRTTDFTSSSCIGQSTAFCLELPVHLNVPDFREN 240
Query: 241 FAYYKEIKGKLTLV-SGSTYSSNVN-LVPVVRPPLNINLPYAILFKINLLVQQGCLPGPA 300
FA Y E + L+ SGS+YSSN N LVPVV PP +LP+ ILFK+N LVQ CL GPA
Sbjct: 241 FANYAEHRASSFLIESGSSYSSNANTLVPVVDPPPGFSLPFEILFKLNTLVQNACLSGPA 300
Query: 301 LDISFYQMVDPQIHNIAFIDHALKKLYHLKECCYNPSKWLDDEYRKYFRLKNPPLPPIFT 360
LD+ FY++++ + ++ A IDH L+KL+HL ECCY P+ WL DEY+K+ PL P +
Sbjct: 301 LDLDFYRLLNQKKYDRALIDHCLEKLFHLGECCYEPAHWLRDEYKKWISKGKLPLSPTIS 360
Query: 361 LNEGLVYVHRVQVTPCKVYFCGPEVNVSNRVLRGYPKYLDNFLRVSFVDEELDKMYSTEL 420
L++GLVY++RVQVTP +VYF GPEVNVSNRVLR Y KY++NFLRVSFVDE+L+K+ S +L
Sbjct: 361 LDDGLVYMYRVQVTPARVYFSGPEVNVSNRVLRHYSKYINNFLRVSFVDEDLEKVRSMDL 420
Query: 421 SPRESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDGLTA 480
SPR S+ +TK++ RI SVL+DGI IGDKKFEFLA+SSSQLREN+ WMFAP D +TA
Sbjct: 421 SPRSST---QRRTKLYDRIYSVLRDGIVIGDKKFEFLAFSSSQLRENSAWMFAPIDRITA 480
Query: 481 AKIRKGMGDFHSIRNVAKYAARLGQSFGSSTETLSVSRREVEVIPDIEVESGSGVNYVFS 540
A IR MGDF IRNVAKYAARLGQSF SS ETL+V E+EVIPD+E+ S G YVFS
Sbjct: 481 AHIRAWMGDFDHIRNVAKYAARLGQSFSSSRETLNVRSDEIEVIPDVEIIS-LGTRYVFS 540
Query: 541 DGIGKISASFARKVAQKCGI-RHTPSAFQIRYAGFKGVISIDPTSSVKLSLRNSMLKYES 600
DGIGKISA FARKVA+KCG+ +PSAFQIRY G+KGV+++DP SS KLSLR SM K+ES
Sbjct: 541 DGIGKISAEFARKVARKCGLTEFSPSAFQIRYGGYKGVVAVDPNSSKKLSLRKSMSKFES 600
Query: 601 ADTKLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQKELIEQLDTIFTDPMKAQQA 660
+TKLDVL+WSKY PC++NRQLITLLSTLGV D VFE KQ+E++++LD I T P++A +A
Sbjct: 601 ENTKLDVLAWSKYQPCYMNRQLITLLSTLGVTDSVFEKKQREVVDRLDAILTHPLEAHEA 660
Query: 661 LELMSPGENTNILKEMILCGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPNGRAMMG 720
L LM+PGENTNILK +ILCGY+PD+EPFLSMML FR SKLLELR K+RIFI GR+MMG
Sbjct: 661 LGLMAPGENTNILKALILCGYKPDAEPFLSMMLQNFRASKLLELRTKTRIFISGGRSMMG 720
Query: 721 CLDETGNLDYGEVFVQCSAHQKLHNDHIIFRRSKSNRHFIVTGTVVVAKNPCLHPGDVRV 780
CLDET L+YG+V VQ S + R FI+TG VVVAKNPCLHPGDVRV
Sbjct: 721 CLDETRTLEYGQVVVQYSDPM------------RPGRRFIITGPVVVAKNPCLHPGDVRV 780
Query: 781 LTAVDVPSLHHMVDCVVFPQRGSRPHPNECSGSDLDGDIYFVCWDPDLIPPQQVEPMDYT 840
L AV+VP+L+HMVDCVVFPQ+G RPHPNECSGSDLDGDIYFVCWD +L+PP+ EPMDYT
Sbjct: 781 LQAVNVPALNHMVDCVVFPQKGLRPHPNECSGSDLDGDIYFVCWDQELVPPRTSEPMDYT 840
Query: 841 PVPSKLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADREPEKAMSKTCIKLAKLFS 900
P P+++LDHDVT+EEV+EYFANY+VNDSLGIIANAHTAFAD+EP KA S CI+LAK FS
Sbjct: 841 PEPTQILDHDVTIEEVEEYFANYIVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKFS 900
Query: 901 IAVDFPKTGVPALISADLRVQAYPDFMDKADKVSYKSDNVLGKLFR-MLDNVGPKIKIRS 960
AVDFPKTGV A+I L V+ YPDFM+K DK +Y+S NV+GKLFR + + P I I+S
Sbjct: 901 TAVDFPKTGVAAVIPQHLYVKEYPDFMEKPDKPTYESKNVIGKLFREVKERAPPLISIKS 960
Query: 961 LTFTREEAQQAYDPDMEVEGFEEYLDDAFYHKNNYDMRLGNLMNYYKIKTEAELISGGSL 1020
FT + A ++YD DMEV+GFEEY+D+AFY K NYD +LGNLM+YY IKTEAE++SGG +
Sbjct: 961 --FTLDVASKSYDKDMEVDGFEEYVDEAFYQKANYDFKLGNLMDYYGIKTEAEILSGGIM 1020
Query: 1021 TKSLSFTKKNEAESITMAVKSLRKEARGWFNENAELHFGHDKNVYARASAWYFVTYHHSY 1080
S SFTK+ +AESI AV++LRKE FN + E ++N A+ASAWY VTYH SY
Sbjct: 1021 RMSKSFTKRRDAESIGRAVRALRKETLSLFNASEE-----EENESAKASAWYHVTYHSSY 1080
Query: 1081 WGWYDDGKKHGHFLSFPWCVHDKLIRIKKRKMNARAR 1114
WG Y++G HFLSF WCV+DKL+RIKK + R R
Sbjct: 1081 WGLYNEGLNRDHFLSFAWCVYDKLVRIKKTNLGRRQR 1093
BLAST of Spg028666 vs. TAIR 10
Match:
AT4G11130.1 (RNA-dependent RNA polymerase 2 )
HSP 1 Score: 761.1 bits (1964), Expect = 1.2e-219
Identity = 435/1125 (38.67%), Postives = 666/1125 (59.20%), Query Frame = 0
Query: 4 TIQLYGFPPGVLQEAVKAFVERFTGEGTIDAIKIKRSKGRGR-RVYATIQFTDDE-GAKL 63
T+++ P ++ + + F+E GE T+ A++I ++ + R +A +QFT E ++
Sbjct: 11 TVKISNVPQTIVADELLRFLELHLGEDTVFALEIPTTRDNWKPRDFARVQFTTLEVKSRA 70
Query: 64 IISLANRRLCYDTSYLKAREWKQDILPDPLVFEYNFKGLRLHLGCQISKE-RFSVLWTES 123
+ + +L + T L+ E DI+P P+ + L +G S E RF L
Sbjct: 71 QLLSSQSKLLFKTHNLRLSEAYDDIIPRPVDPRKRLDDIVLTVGFPESDEKRFCALEKWD 130
Query: 124 TVSVDFGFERRELKFFISHPHVDYMLVLRYENIWQVELHKPQGQS--VDYLLIQLFGAPR 183
V E+R ++F++ Y + +R+E+I + G + +D L++L P+
Sbjct: 131 GVRCWILTEKRRVEFWVWESGDCYKIEVRFEDIIETLSCCVNGDASEIDAFLLKLKYGPK 190
Query: 184 IYERDTKSYRPITEDPFFTF-SEEIDTKWFRATDFTPSCCIGQSAALCLEIPYGRQLPNF 243
+++R T + + F E+ D W R TDF+ S IG S CLE+ G + +
Sbjct: 191 VFKRVTVHIATKFKSDRYRFCKEDFDFMWIRTTDFSGSKSIGTSTCFCLEVHNGSTMLDI 250
Query: 244 HDNFAYYKEIKGKLTLVSGSTYSSNVNLVPVVRPP-LNINLPYAILFKINLLVQ-QGCLP 303
YY+E LT V G T++S +VP++ L + PY ILF++N LV Q
Sbjct: 251 FSGLPYYREDTLSLTYVDGKTFASAAQIVPLLNAAILGLEFPYEILFQLNALVHAQKISL 310
Query: 304 GPALDISFYQMVDPQIHNIAFIDHALKKLYHLKECCYNPSKWLDDEYRKYF-RLKNPPLP 363
A D+ +++ A + LKKL+ CY+P ++ + + ++K+ P
Sbjct: 311 FAASDMELIKILRGMSLETALV--ILKKLHQQSSICYDPVFFVKTQMQSVVKKMKHSPAS 370
Query: 364 PIFTLNE-GLVYVHRVQVTPCKVYFCGPEVNVSNRVLRGYPKYLDNFLRVSFVDEELDKM 423
L E ++ R VTP K+Y GPE+ +N V++ + +++ +F+RV+FV+E+ K+
Sbjct: 371 AYKRLTEQNIMSCQRAYVTPSKIYLLGPELETANYVVKNFAEHVSDFMRVTFVEEDWSKL 430
Query: 424 YSTELS--PRESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFA 483
+ LS +E ++ +T I+ R+LS+L +GIT+G K+FEFLA+S+SQLR N+VWMFA
Sbjct: 431 PANALSVNSKEGYFVKPSRTNIYNRVLSILGEGITVGPKRFEFLAFSASQLRGNSVWMFA 490
Query: 484 PRDGLTAAKIRKGMGDFHSIRNVAKYAARLGQSFGSSTETLSVSRREVEVIPDIEVESGS 543
+ + A IR+ MG F IR+++K AAR+GQ F +S +TL V ++VE IPDIEV +
Sbjct: 491 SNEKVKAEDIREWMGCFRKIRSISKCAARMGQLFSASRQTLIVRAQDVEQIPDIEVTT-D 550
Query: 544 GVNYVFSDGIGKISASFARKVAQKCGIRHTPSAFQIRYAGFKGVISIDPTSSVKLSLRNS 603
G +Y FSDGIGKIS +FA++VAQKCG+ H PSAFQIRY G+KGVI++D +S KLSLR+S
Sbjct: 551 GADYCFSDGIGKISLAFAKQVAQKCGLSHVPSAFQIRYGGYKGVIAVDRSSFRKLSLRDS 610
Query: 604 MLKYESADTKLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQKELIEQLDTIFTDP 663
MLK++S + L+V W++ PCFLNR++I LLSTLG++D +FE+ Q + L + D
Sbjct: 611 MLKFDSNNRMLNVTRWTESMPCFLNREIICLLSTLGIEDAMFEAMQAVHLSMLGNMLEDR 670
Query: 664 MKAQQALELMSPGENTNILKEMILCGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPN 723
A L+ +S + N+L +M+L GY P SEP+LSMML ES+L EL+ + RI +P
Sbjct: 671 DAALNVLQKLSGENSKNLLVKMLLQGYAPSSEPYLSMMLRVHHESQLSELKSRCRILVPK 730
Query: 724 GRAMMGCLDETGNLDYGEVFVQCS-AHQKLHNDHIIFRRSKSNRHFIVTGTVVVAKNPCL 783
GR ++GC+DE G L+YG+V+V+ + +L + + R +V G VVV KNPCL
Sbjct: 731 GRILIGCMDEMGILEYGQVYVRVTLTKAELKSRDQSYFRKIDEETSVVIGKVVVTKNPCL 790
Query: 784 HPGDVRVLTAVDVPSLHH--MVDCVVFPQRGSRPHPNECSGSDLDGDIYFVCWDPDLIPP 843
HPGD+RVL A+ +DC++FPQ+G RPHPNECSG DLDGD +FV WD +IP
Sbjct: 791 HPGDIRVLDAIYEVHFEEKGYLDCIIFPQKGERPHPNECSGGDLDGDQFFVSWDEKIIPS 850
Query: 844 QQVEPMDYTPVPSKLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADREPEKAMSKT 903
+ PMDY +L+DHDVT+EE+ ++F +YM++D+LG+I+ AH ADR+PEKA S+
Sbjct: 851 EMDPPMDYAGSRPRLMDHDVTLEEIHKFFVDYMISDTLGVISTAHLVHADRDPEKARSQK 910
Query: 904 CIKLAKLFSIAVDFPKTGVPALISADLRVQAYPDFMDKADKVSYKSDNVLGKLFRMLDNV 963
C++LA L S AVDF KTG PA + L+ + +PDF+++ +K +Y S++V GKL+R + +
Sbjct: 911 CLELANLHSRAVDFAKTGAPAEMPYALKPREFPDFLERFEKPTYISESVFGKLYRAVKS- 970
Query: 964 GPKIKIRSLTFTREEAQQ----AYDPDMEVEGFEEYLDDAFYHKNNYDMRLGNLMNYYKI 1023
SL + EA+ AYD +E GFE +++ A H++ Y +L +LM YY
Sbjct: 971 -------SLAQRKPEAESEDTVAYDVTLEEAGFESFIETAKAHRDMYGEKLTSLMIYYGA 1030
Query: 1024 KTEAELISGGSLTKSLSFTKKNE-----AESITMAVKSLRKEARGWFNENAELHFGHDKN 1083
E E+++G TK + + N + IT++VK L KEA GWF ++ E ++
Sbjct: 1031 ANEEEILTGILKTKEMYLARDNRRYGDMKDRITLSVKDLHKEAMGWFEKSCE----DEQQ 1090
Query: 1084 VYARASAWYFVTYHHSYWGWYDDGKKHGHFLSFPWCVHDKLIRIK 1105
ASAWY+VTY+ + + D K FLSFPW V D L+ IK
Sbjct: 1091 KKKLASAWYYVTYNPN----HRDEKL--TFLSFPWIVGDVLLDIK 1114
BLAST of Spg028666 vs. TAIR 10
Match:
AT3G49500.1 (RNA-dependent RNA polymerase 6 )
HSP 1 Score: 572.8 bits (1475), Expect = 6.1e-163
Identity = 386/1074 (35.94%), Postives = 570/1074 (53.07%), Query Frame = 0
Query: 95 YNFKGLRLHLGCQISKERFSVLWTESTV---------SVDFGFERR---ELKFFISHP-- 154
Y G+ L +G +S++ F V W V + F F + K + H
Sbjct: 130 YKLAGITLEIGTLVSRDDFFVSWRAEGVDFLVDPFDNTCKFCFRKSTAFSFKDAVMHAVI 189
Query: 155 HVDYMLVLRYENIWQVELHKPQGQSVDYLLIQLFGAPRIYERDTKSYRPITEDPFFTFSE 214
+ DY L L +I V +K V L++QL +PR++ YR +D + T
Sbjct: 190 NCDYKLELLVRDIQTVRQYKTLHGFV--LILQLASSPRVW------YRTADDDIYDTVPG 249
Query: 215 EI---DTKWFRATDFTPSCCIGQ--SAALCLEIPYGRQLPNFHDNFAYYKEIKGKLTLVS 274
++ D W R TDFT IG+ S + + Y +L D F + + ++
Sbjct: 250 DLLDDDDPWIRTTDFTQVGAIGRCHSYRVLISPRYENKLRTALDYFRMRRVQEERV---- 309
Query: 275 GSTYSSNVNLVPVVRPPLNIN---------LPYAILFKINLLVQQGCLPGPALDISFYQM 334
+ + P P++ + + + I+F +N ++ +G L F+ +
Sbjct: 310 --RWPPRIRNEPCFGEPVSDHFFCIHHKEGISFEIMFLVNSVLHRGVFNQFQLTERFFDL 369
Query: 335 V--DPQIHNIAFIDHALKKLYHLKECCYNPSKWLDDEYRKYFRLKNPPLPPIFTLNEGLV 394
+ P+ NIA +LK L K ++ K L + + + KNP L +E +
Sbjct: 370 LRNQPKDVNIA----SLKHLCTYKRPVFDAYKRL--KLVQEWIQKNPKLLGSHEQSEDIS 429
Query: 395 YVHRVQVTPCKVYFCGPEVNVSNRVLRGYPKYLDNFLRVSFVDEELDKMYSTELS----- 454
+ R+ +TP + Y PEV +SNRVLR Y + FLRV+F+DE + + S LS
Sbjct: 430 EIRRLVITPTRAYCLPPEVELSNRVLRRYKAVAERFLRVTFMDESMQTINSNVLSYFVAP 489
Query: 455 --PRESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRENAVWMFAPRDGLT 514
+SS KT +FKR+ S+L DG + +K+ FLA+S++QLR+ + W FA
Sbjct: 490 IVKDLTSSSFSQKTYVFKRVKSILTDGFKLCGRKYSFLAFSANQLRDRSAWFFAEDGKTR 549
Query: 515 AAKIRKGMGDFHSIRNVAKYAARLGQSFGSSTETLSVSRREVEV-IPDIEVESGSGVNYV 574
+ I+ MG F +NVAK AAR+G F S+ T+ V EV+ +PDIE YV
Sbjct: 550 VSDIKTWMGKFKD-KNVAKCAARMGLCFSSTYATVDVMPHEVDTEVPDIERN-----GYV 609
Query: 575 FSDGIGKISASFARKVAQ--KCGIRHTPSAFQIRYAGFKGVISIDPTSS--VKLSLRNSM 634
FSDGIG I+ A +V + K + ++P A+QIRYAGFKGV++ P+ S ++L+LR+SM
Sbjct: 610 FSDGIGTITPDLADEVMEKLKLDVHYSPCAYQIRYAGFKGVVARWPSKSDGIRLALRDSM 669
Query: 635 LKYESADTKLDVLSWSKYHPCFLNRQLITLLSTLGVQDHVFESKQKELIEQLDTIFTDPM 694
K+ S T L++ SW+++ P FLNRQ+ITLLS LGV D +F Q+ ++ +L+ I D
Sbjct: 670 KKFFSKHTILEICSWTRFQPGFLNRQIITLLSVLGVPDEIFWDMQESMLYKLNRILDDTD 729
Query: 695 KAQQALELMSPGENTNILKEMILCGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPNG 754
A + L S E N M+ G++P +EP L ML + R ++L LR KSRIF+ +G
Sbjct: 730 VAFEVL-TASCAEQGNTAAIMLSAGFKPKTEPHLRGMLSSVRIAQLWGLREKSRIFVTSG 789
Query: 755 RAMMGCLDETGNLDYGEVFVQCS--AHQKLHNDH-IIFRRSKSNRHFIVTGTVVVAKNPC 814
R +MGCLDE G L++G+ F+Q S + + + H F+ +K++ +V G V +AKNPC
Sbjct: 790 RWLMGCLDEAGILEHGQCFIQVSKPSIENCFSKHGSRFKETKTDLE-VVKGYVAIAKNPC 849
Query: 815 LHPGDVRVLTAVDVPSLHHMVDCVVFPQRGSRPHPNECSGSDLDGDIYFVCWDPDLIPP- 874
LHPGDVR+L AVDVP LHHM DC++FPQ+G RPH NE SGSDLDGD+YFV WD LIPP
Sbjct: 850 LHPGDVRILEAVDVPQLHHMYDCLIFPQKGDRPHTNEASGSDLDGDLYFVAWDQKLIPPN 909
Query: 875 -QQVEPMDYTPVPSKLLDHDVTMEEVQEYFANYMVNDSLGIIANAHTAFADREPEKAMSK 934
+ M Y K L V +++ ++FA + N+ LG I NAH ADR AM +
Sbjct: 910 RKSYPAMHYDAAEEKSLGRAVNHQDIIDFFARNLANEQLGTICNAHVVHADRSEYGAMDE 969
Query: 935 TCIKLAKLFSIAVDFPKTGVPALISADLRVQAYPDFMDKADKVSYKSDNVLGKLFRMLDN 994
C+ LA+L + AVDFPKTG + L+ + YPDFM K D +YKS+ +LG+L+R +
Sbjct: 970 ECLLLAELAATAVDFPKTGKIVSMPFHLKPKLYPDFMGKEDYQTYKSNKILGRLYRRVKE 1029
Query: 995 VGPKIKIRSLTFTREEAQQAYDPDMEVEGFEEYLDDAFYHKNNYDMRLGNLMNYYKIKTE 1054
V + S + + + YD +E+ GFE+ + +A+ HK YD +L L+ YK++ E
Sbjct: 1030 VYDEDAEASSEESTDPSAIPYDAVLEIPGFEDLIPEAWGHKCLYDGQLIGLLGQYKVQKE 1089
Query: 1055 AELISG--GSLTKSLSFTKKNEAESITMAVKSLRKEARGWFNENAELHFG---HDKNVY- 1107
E+++G S+ K S + E + + SL+KE R F E H +KN+
Sbjct: 1090 EEIVTGHIWSMPKYTSKKQGELKERLKHSYNSLKKEFRKVFEETIPDHENLSEEEKNILY 1149
BLAST of Spg028666 vs. TAIR 10
Match:
AT2G19920.1 (RNA-dependent RNA polymerase family protein )
HSP 1 Score: 123.6 bits (309), Expect = 9.8e-28
Identity = 162/619 (26.17%), Postives = 258/619 (41.68%), Query Frame = 0
Query: 420 PRESSSLEDGKTKIFKRILSVLKDGITIGDKKFEFLAYSSSQLRE-------NAVWMFAP 479
P+ES+ ++ + + + K+GI +G ++++F + E V +
Sbjct: 258 PKESTYCDNP----YSKYKEIAKNGIMVGLRRYQFFVFKDGGKAEKKKRNSTKQVKCYFI 317
Query: 480 RDGLTAAK------IRKGMG---------DFHSIRNVAKYAARLGQSFGSSTETLSVSRR 539
R G TA+ I GM H++ + A Y AR S T+ L V
Sbjct: 318 RTGSTASSDMENPYILSGMSIHEARMHFMHVHTLPSPANYMARFSLIL-SKTKKLEVDMT 377
Query: 540 EVEV--IPDI--------EVESGSGVNYVFSDGIGKISASFARKV--------------A 599
E+ V I DI +V +G + SDG G IS AR
Sbjct: 378 EITVMQIDDIHCHDQSNNDVLDKNGKPRIHSDGTGYISEDLARMCPLNIFKGKSMRSNNI 437
Query: 600 QKCGIRHTPSAFQIRY----AGFKGVISID---PTSSVKLSLRNSMLK-YE-------SA 659
Q ++ P QIR + KG+ ++ P +V+ +R SM+K Y+ S
Sbjct: 438 QGTCVQEPPLLIQIRMFNDGSAVKGIFLLNKNLPPQTVQ--VRPSMIKVYKDKNLSNFST 497
Query: 660 DTKLDVLSWSK-YHPCFLNRQLITLLSTLGVQDHVFESKQKELIEQLDTIFTDPMKAQQA 719
L+V++ S L++ L+ LLS GV + F +E+ TIF A +A
Sbjct: 498 FNSLEVVTTSNPPKRAKLSKNLVALLSYGGVPNDFFLDILLNTLEKKKTIFFKVRAAGKA 557
Query: 720 LELMSPGENTNILKEMILCGYRPDSEPFLSMMLHTFRESKLLELR----RKSRIFIPNGR 779
++ N L +MI+ G P EP+L L SKLL+L + ++ I
Sbjct: 558 ALHYGNMDDKNAL-QMIMAGI-PLDEPYLKHYL-----SKLLKLEKDDLKAGKLPIDESY 617
Query: 780 AMMGCLDETGNLDYGEVFVQCSAHQKLHNDHIIFRRSKSNRHFIVTGTVVVAKNPCLHPG 839
+MG +D TG L EV + V+V +NP LH G
Sbjct: 618 YLMGTVDPTGELKEDEVSGLAKSQD-----------------------VLVYRNPGLHFG 677
Query: 840 DVRVLTAVDVPSLHHMVD----CVVFPQRGSRPHPNECSGSDLDGDIYFVCWDPDLIPPQ 899
D+ +L A V SL V V FPQ+G R +E +G D DGD+YF+ +P L+ +
Sbjct: 678 DIHILKATYVKSLEQYVGNSKYGVFFPQKGPRSLGDEIAGGDFDGDMYFISRNPKLL--E 737
Query: 900 QVEPMD-----------YTPVPSKLLDHDVTMEEVQEYF--ANYMVNDSLGIIANAHTAF 947
+P + YT L + EE+ + F + + +G A++ A
Sbjct: 738 HYKPSEPWVSSSPRSKIYTGRQPSELSPEQLEEELFKIFLKTGFSPSSVIGQAADSWLAI 797
BLAST of Spg028666 vs. TAIR 10
Match:
AT2G19910.1 (RNA-dependent RNA polymerase family protein )
HSP 1 Score: 118.2 bits (295), Expect = 4.1e-26
Identity = 107/394 (27.16%), Postives = 173/394 (43.91%), Query Frame = 0
Query: 615 LNRQLITLLSTLGVQDHVFESKQKELIEQLDTIFTDPMKAQQALELMSPGENTNILKEMI 674
L+R L+ LLS GV + F + + +E+ TIF A +A + T +MI
Sbjct: 517 LSRNLVALLSYGGVPNDFFLNILRNTLEESKTIFYSERAAFKAAINYGDDQYT---ADMI 576
Query: 675 LCGYRPDSEPFLSMMLHTFRESKLLELRRKSRIFIPNGRAMMGCLDETGNLDYGEVFVQC 734
L G P EP+L L +++ L + R I +MG +D TG L E+ V
Sbjct: 577 LVGI-PLDEPYLKDRLSYLLKTERNAL-KAGRFPIDESYYIMGTVDPTGELKENEICVIL 636
Query: 735 SAHQKLHNDHIIFRRSKSNRHFIVTGTVVVAKNPCLHPGDVRVLTAVDVPSLHHMVD--- 794
+ Q ++G V+V +NP LH GD+ VL A V +L V
Sbjct: 637 HSGQ-------------------ISGDVLVYRNPGLHFGDIHVLKATYVKALEDYVGNAK 696
Query: 795 -CVVFPQRGSRPHPNECSGSDLDGDIYFVCWDPDLIPP-QQVEPMDYTPVPSKL------ 854
V FPQ+G R +E +G D DGD+YF+ +P L+ + EP + PSK+
Sbjct: 697 FAVFFPQKGPRSLGDEIAGGDFDGDMYFISRNPKLLEHFKPSEPWVSSSKPSKIYCGRKP 756
Query: 855 --LDHDVTMEEVQEYF--ANYMVNDSLGIIANAHTAFAD---------REPEKAMSKTCI 914
L + EE+ + F A + D +G+ A+ D + + K +
Sbjct: 757 SELSEEELEEELFKMFLKARFCKRDVIGMAADCWLGIMDPFLTLGDESAKEKYERKKNIL 816
Query: 915 KLAKLFSIAVDFPKTGVPALISADLRVQAYPDFMDKADKVSYKSDNVLGKLFRMLDNVG- 974
KL ++ A+D PK G + DL ++ +P +M++ K ++S ++LG +F +D+
Sbjct: 817 KLIDIYYDALDAPKKGAKVDLPPDLEIKNFPHYMERDPKRDFRSTSILGLIFDTVDSHNA 876
Query: 975 ---PKIKIRSLTFTREEAQQAYDPDMEVEGFEEY 981
P +I L + +E D +E Y
Sbjct: 877 EEPPPSEISKLWYFEDEPVPKSHMDKFTSWYENY 886
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038877934.1 | 0.0e+00 | 88.16 | RNA-dependent RNA polymerase 1-like isoform X1 [Benincasa hispida] >XP_038877936... | [more] |
XP_022943176.1 | 0.0e+00 | 87.89 | RNA-dependent RNA polymerase 1-like [Cucurbita moschata] | [more] |
XP_023539816.1 | 0.0e+00 | 87.89 | RNA-dependent RNA polymerase 1-like [Cucurbita pepo subsp. pepo] | [more] |
KAG6599932.1 | 0.0e+00 | 87.89 | RNA-dependent RNA polymerase 1, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
NP_001267682.1 | 0.0e+00 | 87.35 | RNA-dependent RNA polymerase 1-like [Cucumis sativus] >XP_011654424.1 RNA-depend... | [more] |
Match Name | E-value | Identity | Description | |
Q9LQV2 | 0.0e+00 | 60.88 | RNA-dependent RNA polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=RDR1 PE=2 SV=1 | [more] |
Q0DXS3 | 9.5e-262 | 61.43 | Probable RNA-dependent RNA polymerase 1 OS=Oryza sativa subsp. japonica OX=39947... | [more] |
O82504 | 1.7e-218 | 38.67 | RNA-dependent RNA polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=RDR2 PE=1 SV=1 | [more] |
Q7XM31 | 2.3e-207 | 37.32 | Probable RNA-dependent RNA polymerase 2 OS=Oryza sativa subsp. japonica OX=39947... | [more] |
Q9SG02 | 8.6e-162 | 35.94 | RNA-dependent RNA polymerase 6 OS=Arabidopsis thaliana OX=3702 GN=RDR6 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1FR02 | 0.0e+00 | 87.89 | RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111447986 PE=3 ... | [more] |
E9NWK7 | 0.0e+00 | 87.35 | RNA-dependent RNA polymerase OS=Cucumis sativus OX=3659 GN=Csa_5G239640 PE=2 SV=... | [more] |
A0A6J1K2Q9 | 0.0e+00 | 87.26 | RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111490531 PE=3 SV... | [more] |
H3K3Y9 | 0.0e+00 | 86.91 | RNA-dependent RNA polymerase OS=Cucumis sativus OX=3659 GN=RDR1 PE=2 SV=1 | [more] |
A0A0S1YCZ7 | 0.0e+00 | 87.00 | RNA-dependent RNA polymerase OS=Cucumis sativus OX=3659 GN=RDR1b PE=2 SV=1 | [more] |