Spg005020 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg005020
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRibonuclease H
Locationscaffold4: 10856897 .. 10859420 (-)
RNA-Seq ExpressionSpg005020
SyntenySpg005020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAGATCTAAGTCTGGCGGACGACTTGGCCCTGAAAAATCCGGATCCAAGCAACAAAAGGGCCCACAGAAGTTTGCTTCCAAGGCCGGTGGCTGATGTGCGTGCCAAGGTCGGATCTAAGGGAAAGATGAGGGCCCGAATGCAGTCAGGAATTGAGCACGACGATGTCGATCGGGGGCTAAAGTTGCCAAAGTTGAGAAGGGCAGATAAAGAAGACCTAGAGGACTTGATAGGACAAGCAGCTCCACCGTATACCAATGACATCATGGAAGCAGAGGTACCCCAAAAGTTCAAGATACCAAATTTTCAGTAGTGCAATGGCAAGAAGGATCCAGTTCAACACTTAGATACCTACAGATCTTGGATGGATTTCCATGGGGTGTCTAAAGCAACCAAGTGTTGAGCTTTCTTCCTTACTCTAACGGGGATAGCCAGACAGTGGTTCAGAAAGCTACCTCGAAAGTCTATAGGGTCGTTCAAGGAGTTGGCACGGGCCTTTGTCACATAATTCCTTGGAGCAAGAGACCGGCGCAAGCCACAAATCAACCTACTGACCATTAAGCAAAGGCCAGGAGAAAGCCTGCGAGAATATATAACAAGACTCGGCAACGAGGTATTACAAGTGGAAGGATATGATGATGGAGTTGCACTAACAGCTGTTATTTCAGGTTTGCAGGACGAGAAACTTCTAAATTCTATTGGTGAAAATCAGCCAAGCACCTATGTGGAGTTCATGTCTAGGGCACAAAGATATATAAGCACTGAAGAGTTGTTGAAATCAAAGCAGATAGAAAGAGAAGCCCAGAGGATGGTTGTTTTTTATCGAAAAAAAATATAGAGGAAAAAAACGCCAGAGTGATGTGGGGAGCCAAGACCGTGCAAGATATTCCTCTAGTCGAGGCCGAGCAGATCCCAAAGAAGGTCGAGGGCAAGGTGACCTAATGATAGCGAGCATAGCTAGCTATTTTTCCTAATTAATTTAGCTAGTCTTTAATAATAAATTGGCCAAATTTATGCTTTTTATTGTTCTATTTGTAGGAAATATGTATTGGAGCTAAATTGAGAAATTGGGAATGATCGGGTGTTCGAGGCATGAAAAAAGGCCAAAGAAACGAAGTTGGATCGAGTTGGAAGCTTATTAGACTAGTGCAAACCGAGGACCAGCGTCGAGACCCTAGATCTTGGGCGTCTCGACGTTGCACACTTTCCTTCTCTAATTTGGGCGGCAGAAGACACAACGTCGGGACGTTGTGTCAATAGCATCTCGACGCTCTCGCATAATTCCTAATCAGAATGGGCGGTATCGCGACAGCGTCACGACGCTGTCGACTTTTTTTTTTTCTATAAATAGAATGTGATTATGGCTGATGAAGGGGACCTCTTTTGATGCAATTTTGGCCTAAATTCCGACTAATACCTTCTGTGGGAGCTGAAGCAAGTTGGAGGAGCGGATTTCGAAGGCTCGGGTTGTGGAGATCGTGACGGGGACGTGCGGTTTCGGCGACTTAGCGTTTAATCTCTTTTCCCTCTTGATTTTAGTTAGTTTTAATGGCTGATTTAGACTTAGTTTATACCGTAACGCGTTGTATGAATAGCTAAGTTCTTAATCTTAGGGATTGATGTAGCCGTTGGGCAATTTTGGATTGATTTATGGATGTATTTTATGCTTTCATTTATGCATAACGTGTTCTTAATGCTTTCAATTTACCGGCCATAAATTGATTGATGATTGTGTTTGAATCTTGCTCGGGAGAGGGGATTTAACTTAGAAATCGTTATGCATGACTAGGATTAATCTTGAGATCAGGAGATAATAGATAATTCTAGAAATCGTTAAAAAAGACTAACCTTAAAGCCTTGCATGTTAATGAATTTAGATAGGGAAATTGGGTTTATTGCATGTGAGAGGATTATGTATGCTCGGGAGAGGTATGTATGATTGTAGTTTTCCTAGATTTATGAATCATAGCATGTTAAGTATCCATTAATTAATCCATAATCCCAATTGGTGAAATCAACTCCCTAAGACTTTTGCCATCAATTGATTGTCACACTTTTAGCTTTGTGTTAATTTTGTTTCCATTCTCATCAAGCTTAATTCCCTAGATAAAATTGGATTAGAGTATTTACGGTAGTTAAATTGATTAGAAACTCAATCCAGAGGACGACATTCGAATTAAAATTCACTATATTATAACTTGACCCGTACACTTGCTGTAAGCAAAATCGCACATCACTTAAAAGGAAGATTTGAGAAGTACACCCCACTGTCCGTCCCGCCTTATCAAGTCTTAGCAGCAGTGCAGAACATAGATCTGTTGAAGCGCCCAGAAAGACTCAAGACTAATCCAGATAGAAGAAATAAGAACAAGTATTGCATGTTCCACTGTGATCATGGGCACACAACGCGAAATTGCATACAGTTACGAGATGAGATAGAAAGTTTAATCAATGAGGGTTACCTGAAGGAGTTTGTTAACGATGGCAACAATAAAAGACCAGCTAGAAGCGAACGAGGCTAA

mRNA sequence

ATGCCAGATCTAAGTCTGGCGGACGACTTGGCCCTGAAAAATCCGGATCCAAGCAACAAAAGGGCCCACAGAAGTTTGCTTCCAAGGCCGGTGGCTGATGTGCGTGCCAAGGTCGGATCTAAGGGAAAGATGAGGGCCCGAATGCAGTCAGGAATTGAGCACGACGATGTCGATCGGGGGCTAAAGTTGCCAAAGTTGAGAAGGGCAGATAAAGAAGACCTAGAGGACTTGATAGGACAAGCAGCTCCACCGTATACCAATGACATCATGGAAGCAGAGAGTGATGTGGGGAGCCAAGACCGTGCAAGATATTCCTCTAGTCGAGGCCGAGCAGATCCCAAAGAAGGTCGAGGGCAAGATAGGGAAATTGGGTTTATTGCATGTGAGAGGATTATGTATGCTCGGGAGAGCAAAATCGCACATCACTTAAAAGGAAGATTTGAGAAGTACACCCCACTGTCCGTCCCGCCTTATCAAGTCTTAGCAGCAGTGCAGAACATAGATCTGTTGAAGCGCCCAGAAAGACTCAAGACTAATCCAGATAGAAGAAATAAGAACAAGTATTGCATGTTCCACTGTGATCATGGGCACACAACGCGAAATTGCATACAGTTACGAGATGAGATAGAAAGTTTAATCAATGAGGGTTACCTGAAGGAGTTTGTTAACGATGGCAACAATAAAAGACCAGCTAGAAGCGAACGAGGCTAA

Coding sequence (CDS)

ATGCCAGATCTAAGTCTGGCGGACGACTTGGCCCTGAAAAATCCGGATCCAAGCAACAAAAGGGCCCACAGAAGTTTGCTTCCAAGGCCGGTGGCTGATGTGCGTGCCAAGGTCGGATCTAAGGGAAAGATGAGGGCCCGAATGCAGTCAGGAATTGAGCACGACGATGTCGATCGGGGGCTAAAGTTGCCAAAGTTGAGAAGGGCAGATAAAGAAGACCTAGAGGACTTGATAGGACAAGCAGCTCCACCGTATACCAATGACATCATGGAAGCAGAGAGTGATGTGGGGAGCCAAGACCGTGCAAGATATTCCTCTAGTCGAGGCCGAGCAGATCCCAAAGAAGGTCGAGGGCAAGATAGGGAAATTGGGTTTATTGCATGTGAGAGGATTATGTATGCTCGGGAGAGCAAAATCGCACATCACTTAAAAGGAAGATTTGAGAAGTACACCCCACTGTCCGTCCCGCCTTATCAAGTCTTAGCAGCAGTGCAGAACATAGATCTGTTGAAGCGCCCAGAAAGACTCAAGACTAATCCAGATAGAAGAAATAAGAACAAGTATTGCATGTTCCACTGTGATCATGGGCACACAACGCGAAATTGCATACAGTTACGAGATGAGATAGAAAGTTTAATCAATGAGGGTTACCTGAAGGAGTTTGTTAACGATGGCAACAATAAAAGACCAGCTAGAAGCGAACGAGGCTAA

Protein sequence

MPDLSLADDLALKNPDPSNKRAHRSLLPRPVADVRAKVGSKGKMRARMQSGIEHDDVDRGLKLPKLRRADKEDLEDLIGQAAPPYTNDIMEAESDVGSQDRARYSSSRGRADPKEGRGQDREIGFIACERIMYARESKIAHHLKGRFEKYTPLSVPPYQVLAAVQNIDLLKRPERLKTNPDRRNKNKYCMFHCDHGHTTRNCIQLRDEIESLINEGYLKEFVNDGNNKRPARSERG
Homology
BLAST of Spg005020 vs. NCBI nr
Match: XP_022159192.1 (uncharacterized protein LOC111025612 [Momordica charantia])

HSP 1 Score: 100.5 bits (249), Expect = 2.0e-17
Identity = 48/101 (47.52%), Postives = 67/101 (66.34%), Query Frame = 0

Query: 143 LKGRFEKYTPLSVPPYQVLAAVQNIDLLKRPERLKTNPDRRNKNKYCMFHCDHGHTTRNC 202
           L  +FEKYTP +VP  QVL  +++  LLK PE +K  P++R+K +YC+FH DHGH T +C
Sbjct: 77  LPRKFEKYTPTTVPLEQVLMEIKDQRLLKWPETMKAPPNKRSKGRYCLFHRDHGHATLDC 136

Query: 203 IQLRDEIESLINEGYLKEFVND--------GNNKRPARSER 236
             L++E+E LI +GYLKE+V+D         +NK P R  R
Sbjct: 137 FDLKEEVEGLIRKGYLKEYVDDLKATPNRENDNKSPTREIR 177

BLAST of Spg005020 vs. NCBI nr
Match: XP_022158830.1 (uncharacterized protein LOC111025293 [Momordica charantia])

HSP 1 Score: 99.8 bits (247), Expect = 3.4e-17
Identity = 55/133 (41.35%), Postives = 76/133 (57.14%), Query Frame = 0

Query: 110 RADPKEGRGQDREIGFIACERIMYARESKIAHHLKGRFEKYTPLSVPPYQVLAAVQNIDL 169
           R DPK  R  D+  G          R+         +FEKYTP +VP  QVL  +++  L
Sbjct: 364 RTDPKRERSGDKPQG-----SRWEKRDRSSQKDPPRKFEKYTPTTVPIEQVLMEIKDQRL 423

Query: 170 LKRPERLKTNPDRRNKNKYCMFHCDHGHTTRNCIQLRDEIESLINEGYLKEFV------- 229
           LK PER+K +  +R+K +YC+FH DHGH T++C  L++E+E LI  GYLKE+V       
Sbjct: 424 LKWPERMKASSAKRSKGRYCLFHRDHGHATQDCFDLKEEVEGLIRRGYLKEYVEEPKATQ 483

Query: 230 NDGNNKRPARSER 236
           N  ++K PAR  R
Sbjct: 484 NGESDKSPAREIR 491

BLAST of Spg005020 vs. NCBI nr
Match: XP_024042801.1 (uncharacterized protein LOC112099618 [Citrus clementina])

HSP 1 Score: 99.4 bits (246), Expect = 4.5e-17
Identity = 46/95 (48.42%), Postives = 66/95 (69.47%), Query Frame = 0

Query: 136 ESKIAHHLKGRFEKYTPLSVPPYQVLAAVQNIDLLKRPERLKTNPDRRNKNKYCMFHCDH 195
           E K    L+ RF +YTPL++P  QVL  ++N+DLL++P+ +K  P+RR+++KYC +H DH
Sbjct: 194 EKKGPKILQSRFPRYTPLNLPEEQVLMQIRNLDLLRKPKDIKIAPNRRDRSKYCRYHRDH 253

Query: 196 GHTTRNCIQLRDEIESLINEGYLKEFV-NDGNNKR 230
            H   +C  L++EI+SLI  GYLKEFV  DG   R
Sbjct: 254 RHNIEDCFDLKEEIKSLIQRGYLKEFVRKDGGRIR 288

BLAST of Spg005020 vs. NCBI nr
Match: MQL77781.1 (hypothetical protein [Colocasia esculenta])

HSP 1 Score: 99.0 bits (245), Expect = 5.9e-17
Identity = 46/89 (51.69%), Postives = 62/89 (69.66%), Query Frame = 0

Query: 147 FEKYTPLSVPPYQVLAAVQNIDLLKRPERLKTNPDRRNKNKYCMFHCDHGHTTRNCIQLR 206
           F  YTPL+V P Q+LA ++N   L+ P+R++++P +R++NKYC FH DHGH T  C QL+
Sbjct: 461 FTNYTPLTVAPEQILAEIRNEAFLRWPQRMRSDPRKRDQNKYCRFHRDHGHDTSECRQLK 520

Query: 207 DEIESLINEGYLKEFVNDGNNKRPARSER 236
           DEIE LI  GYL  FV   N +RP R +R
Sbjct: 521 DEIEDLIKRGYLGRFVR-RNEERPRRRDR 548

BLAST of Spg005020 vs. NCBI nr
Match: MQL93545.1 (hypothetical protein [Colocasia esculenta])

HSP 1 Score: 99.0 bits (245), Expect = 5.9e-17
Identity = 47/89 (52.81%), Postives = 61/89 (68.54%), Query Frame = 0

Query: 147 FEKYTPLSVPPYQVLAAVQNIDLLKRPERLKTNPDRRNKNKYCMFHCDHGHTTRNCIQLR 206
           F  YTPL+V P Q+LA ++N   ++ P+R++++P RR++NKYC FH DHGH T  C QL+
Sbjct: 422 FTNYTPLTVAPEQILAEIRNEAYVRWPQRMRSDPRRRDQNKYCRFHRDHGHDTSECRQLK 481

Query: 207 DEIESLINEGYLKEFVNDGNNKRPARSER 236
           DEIE LI  GYL  FV   N  RP R ER
Sbjct: 482 DEIEDLIKRGYLGRFVR-RNEDRPRRRER 509

BLAST of Spg005020 vs. ExPASy TrEMBL
Match: A0A6J1DZ52 (uncharacterized protein LOC111025612 OS=Momordica charantia OX=3673 GN=LOC111025612 PE=4 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 9.8e-18
Identity = 48/101 (47.52%), Postives = 67/101 (66.34%), Query Frame = 0

Query: 143 LKGRFEKYTPLSVPPYQVLAAVQNIDLLKRPERLKTNPDRRNKNKYCMFHCDHGHTTRNC 202
           L  +FEKYTP +VP  QVL  +++  LLK PE +K  P++R+K +YC+FH DHGH T +C
Sbjct: 77  LPRKFEKYTPTTVPLEQVLMEIKDQRLLKWPETMKAPPNKRSKGRYCLFHRDHGHATLDC 136

Query: 203 IQLRDEIESLINEGYLKEFVND--------GNNKRPARSER 236
             L++E+E LI +GYLKE+V+D         +NK P R  R
Sbjct: 137 FDLKEEVEGLIRKGYLKEYVDDLKATPNRENDNKSPTREIR 177

BLAST of Spg005020 vs. ExPASy TrEMBL
Match: A0A6J1DWY0 (uncharacterized protein LOC111025293 OS=Momordica charantia OX=3673 GN=LOC111025293 PE=4 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 1.7e-17
Identity = 55/133 (41.35%), Postives = 76/133 (57.14%), Query Frame = 0

Query: 110 RADPKEGRGQDREIGFIACERIMYARESKIAHHLKGRFEKYTPLSVPPYQVLAAVQNIDL 169
           R DPK  R  D+  G          R+         +FEKYTP +VP  QVL  +++  L
Sbjct: 364 RTDPKRERSGDKPQG-----SRWEKRDRSSQKDPPRKFEKYTPTTVPIEQVLMEIKDQRL 423

Query: 170 LKRPERLKTNPDRRNKNKYCMFHCDHGHTTRNCIQLRDEIESLINEGYLKEFV------- 229
           LK PER+K +  +R+K +YC+FH DHGH T++C  L++E+E LI  GYLKE+V       
Sbjct: 424 LKWPERMKASSAKRSKGRYCLFHRDHGHATQDCFDLKEEVEGLIRRGYLKEYVEEPKATQ 483

Query: 230 NDGNNKRPARSER 236
           N  ++K PAR  R
Sbjct: 484 NGESDKSPAREIR 491

BLAST of Spg005020 vs. ExPASy TrEMBL
Match: A0A6J1DDR5 (uncharacterized protein LOC111020115 OS=Momordica charantia OX=3673 GN=LOC111020115 PE=4 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.4e-16
Identity = 60/178 (33.71%), Postives = 98/178 (55.06%), Query Frame = 0

Query: 72  EDLEDLIGQAAP-PYTNDIMEAESDVGSQDRARYSSSRGRADPKEGR-GQDRE------- 131
           E L   +G+ AP  +T  + +A+  +  Q+  R  + R      +GR G+D+E       
Sbjct: 11  ETLTVKLGEEAPATFTEVLQKAKKVIDGQELIRIKTGRPEKKIDKGRAGKDKEKTDSKSK 70

Query: 132 -IGFIACERIMYARESKIAHHLKGRFEKYTPLSVPPYQVLAAVQNID---LLKRPERLKT 191
             G  +       R S   H+    +E+YTP ++P  ++L  ++  +   LLKRPE+L+ 
Sbjct: 71  DKGPSSSSSRDDYRRSDSTHNRSRPYERYTPTTIPISEILTNIEESEMEKLLKRPEKLRG 130

Query: 192 NPDRRNKNKYCMFHCDHGHTTRNCIQLRDEIESLINEGYLKEFV-NDGNNKRPARSER 236
           +P++RNK+KYC+FH DHGH+T NC +L+ +IE LI + Y K+FV    +N    + ER
Sbjct: 131 DPEKRNKDKYCLFHRDHGHSTSNCWELKRQIEDLIQDDYFKKFVGKPRSNSAEKKDER 188

BLAST of Spg005020 vs. ExPASy TrEMBL
Match: A0A6J1CNT2 (uncharacterized protein LOC111012805 OS=Momordica charantia OX=3673 GN=LOC111012805 PE=4 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 4.1e-16
Identity = 46/97 (47.42%), Postives = 64/97 (65.98%), Query Frame = 0

Query: 146 RFEKYTPLSVPPYQVLAAVQNIDLLKRPERLKTNPDRRNKNKYCMFHCDHGHTTRNCIQL 205
           +FEKYT  +VP  QVL  ++N  LLK PER+  +  +R+K +YC+FH DHGH T++C  L
Sbjct: 220 KFEKYTSTTVPLEQVLMEIKNQRLLKWPERMNASSTKRSKGRYCLFHWDHGHATQDCFDL 279

Query: 206 RDEIESLINEGYLKEFV-------NDGNNKRPARSER 236
           ++E+E LI  GYLKE+V       N  ++K PAR  R
Sbjct: 280 KEEVEGLILRGYLKEYVEEPKATQNGESDKSPAREIR 316

BLAST of Spg005020 vs. ExPASy TrEMBL
Match: A0A6J1DHB3 (uncharacterized protein LOC111020479 OS=Momordica charantia OX=3673 GN=LOC111020479 PE=4 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 5.4e-16
Identity = 49/138 (35.51%), Postives = 78/138 (56.52%), Query Frame = 0

Query: 100 DRARYSSSRGRADPKEGRGQDREIGFIACERIMYARESKIAHHLKGRFEKYTPLSVPPYQ 159
           D+ R    +G+AD K      R+ G  +    +  R S  +H+    +E YTP ++P ++
Sbjct: 303 DQGRAGKDKGKADSK-----SRDKGPSSSSSRVDYRRSNSSHNQSRPYEHYTPTTIPIFE 362

Query: 160 VLAAVQNI---DLLKRPERLKTNPDRRNKNKYCMFHCDHGHTTRNCIQLRDEIESLINEG 219
           +L  ++      LLKRPE+L+ +P++RN +KYC FH DHGH T N  +L+ +IE LI +G
Sbjct: 363 ILTNIEETGMEKLLKRPEKLRGDPEKRNTDKYCRFHRDHGHNTSNYWELKRQIEDLIQDG 422

Query: 220 YLKEFVNDGNNKRPARSE 235
           Y K+FV    +    + E
Sbjct: 423 YFKKFVGKPRSNSVEKKE 435

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022159192.12.0e-1747.52uncharacterized protein LOC111025612 [Momordica charantia][more]
XP_022158830.13.4e-1741.35uncharacterized protein LOC111025293 [Momordica charantia][more]
XP_024042801.14.5e-1748.42uncharacterized protein LOC112099618 [Citrus clementina][more]
MQL77781.15.9e-1751.69hypothetical protein [Colocasia esculenta][more]
MQL93545.15.9e-1752.81hypothetical protein [Colocasia esculenta][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DZ529.8e-1847.52uncharacterized protein LOC111025612 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A6J1DWY01.7e-1741.35uncharacterized protein LOC111025293 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A6J1DDR51.4e-1633.71uncharacterized protein LOC111020115 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A6J1CNT24.1e-1647.42uncharacterized protein LOC111012805 OS=Momordica charantia OX=3673 GN=LOC111012... [more]
A0A6J1DHB35.4e-1635.51uncharacterized protein LOC111020479 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 14..40
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..59
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 88..118

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg005020.1Spg005020.1mRNA