Moc05g17140 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g17140
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPol protein
Locationchr5: 12800694 .. 12803721 (+)
RNA-Seq ExpressionMoc05g17140
SyntenyMoc05g17140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGGTCCTTCTTTTTATAGCCTCGGGCAATCGACCAGGAAGAACGACGGTTAGGGATGGCAGAGACGCGCCACGCTGTACGGGAAGCATCGAGGAGCATGCCTCTAATAGTTGCGGGCCGACACCTCGTCCTACTGCTCAGAGCCTGTTGCCTGCAGCTATGAGGTATGCATGTCGTATTGATGGTATCCGCCACCCACTACGATTACGTACAACGTCCAATTGTCCACATTCCCTTATCTCTTCGTGAGAGGACATGTGTCCTGTTCGGCAATGCTAGTTTGTGGGGGAGGGCAACCGTATGTGGTCTTGCGTTCCAGAAGCAGACACACATCCTACTCGATTTCTTTCCCCCAACAAATATAAATAAAAACAAAGGAATCTCGAAACTGATGATTCGAAGAGGAAAAAAGATAGATCGATACAATAATTAATAGAAAACATATTACCTATGTGTAACGACAAAGTATAAATTGAAAGAAAAAAAGGTTGACCTTGAAATTGCCAGCAACAATTAGATCAAAGAGACGACAATGATGTATTCTAGTAATATATATATATATATATATATATATATATATATATATATATATATTTTAAATATTCCACTCATTTATAGATATTTTTTCATTTTTTTAAAAATTGTCGTATTTGTTTAGGAAATGATATGTTTCCAATTTCTTTTAGTTTTTCAAAAATTAACTTAATTATTTAATTTTCAAAAAATATTTTCCTTTTTTTAACCTCGGATAAATTTGGTAATAAATATATAATTGTTGCCAAAATCTGGTAACAAATAATTTAAAAAAAAAAAGAAAGAAAAGAAAGTGGATGAAGCTAGGGTCCCAAGGACGTCGGAGCCACCAGGGGGCTTGAGCACGCATGGACCACTGTGTGAATACATATTTTGTTGTTTAGAAATCAATCCGTATACATTAATTATATACTTTACGAAGTTTAATGGCCCTGGGTCCTTAATAAAACAGCCCCTGCTAGTAGGATCTTTAAACTTTTAATTTTATGTCTTATGTTCTTAAGTTTTAAAAAGAATTCAATAGGTCCTAAAAACTTTTAACATTATGTTTAATGGGTTTTAAATTTTAATAGGTATAAAAGAACAAGAGAATTTATTATTCAAGATCATTTTAAATATAAAATTGAAAGTTCAAATATCTATTAGACAATTTTTTAAAGTTATCTATCGAGTATTGTTTAGAAAAGTATTTTCCATCTACCGAGTATTGTTTAGAAAAGTATTTGCCAATCTCTAATATTTAACTTTAGTCTATTAGACACGACCTTAAAATTTTATGAATAATTTCACCCAAATACTCTTAAGGAAAAAAAAATCACCCAAATACTTTTTAGATTAGCTTTTTGTTTTTGTTTAATTACTTTCAACAGCCTTAAATTTTGAACATAGCAATCAATGATGACCAACATCCATGACTGAACGGTTAAGATATTTTGCTATTTCTTAGGGGTATCTATTAATAATGTATTGGACGAGTTGTTTTGAAAACTTATTTCCACATATCTCTAATGTTTACAAATTTTATAGTTTTAGATTTTTGGATTTATAAATTTTCACTATTGCCAAATTTGTCCGCATTTATATTTATTATAATTAGTTTTTTTATTTAAATGTAAAAGATTTAATGTTTCAAATTTGATTTTATAAACGTTAAATTATTGTGGGACCCAAATCAGTTATTCTTGTAATCTCCTAATTTACTCTTTAATATATTTTACTAAAGAAGGAAATTTATTACAAAATATTTTATGATTTCATTCCAATTTTTTTTTTCGCTCAATTTCAATATATTTCAAATGCAATATTTGGCTCAATCTCACTAAAATTTGTCGAAGAGCACTACATTTTAGGAGAGTAAACTTGCCGGCATGATGAGGTAAGACGACAATGGAGTAATGCGACTTCATCCTTTATTTATATATATATATATATATATCTTCTTTTTTGTTTATCGCCTAAATTTTCATTTATATGCAGTTATAAACTATATTCGACTATAAACTATAGCCATTATACCATAAACTTATATGAAGATATAAACTATACTTGGCTATAAACTATAACTTACTATAAAGTATATACGACTATATCCCTCTATAATTAACAAATAAAACAAATAGCCTACATAATCAAATACATACGACGTCATCATCGAATATTTTTTCAATACCCCAAAATCGAACACCCAAAATCCGAAATAAAAAACAAACCTAGAATATTTAACTTACATAATCAATTGCAAACGGGCTGCCGAAGACGGTAACGACAAAAAACTGAGAACACTAAGAAGACGAGGAGATGGCCTGCTGGAAACAATGGTGAAAGCAAGAGAAAAAGAAGATAATGAAGAAGAGGAAGAGATAAGTAAAAATTTGGGAAGTTTTTTTTTTAGGAAACTGAAAAGGTGCATAAATCTAAGAATTTAAAATGTAAACCCCAAATCAACAATTTTTCAATTTTGAATTTTCATATCTTAATAATAATTATATGTGTATAAAAATGGTATATTGGTAAAATGCAGATGAGTTTCATCTTAAGGGGTGTATTTTAAGGCAAAAGTATAAATTTTGTTGAATCCCTTTGTGTAATTATCATTTCCAAATGGTCTATCAACATACTAATCCCTAATAATAATTGTCAATTTTTAATGGCTCTATTCACTAGGGGTGAAAGAAATCGAACCGACCCGAAAAACCAAACCGAACCGAATTGGTTCGGGTCCGGTCGGTTTATATAAAATATGAACCGAAACCGAAGGAAAGGGAAGCTTATTCCACGCTTTATTGGGCCATTTGAAGTGTTGGAGCATATTGGCTTCGTACCTTATCGATTGGTTTTACCGCCTTCCCTTTCAGTAGTCCACAATGTCTATCATGTATCCTTGTTATGGAAGTACATGGCGGATCCATCTCATGTCATCGACTACAAGCCATTGTGTTTGAGTGAGAACTTGAGTTATGAAGAGAAGCCAAGACGAATTCTTGCTAGATAA

mRNA sequence

ATGAGGGTCCTTCTTTTTATAGCCTCGGGCAATCGACCAGGAAGAACGACGGTTAGGGATGGCAGAGACGCGCCACGCTGTACGGGAAGCATCGAGGAGCATGCCTCTAATAGTTGCGGGCCGACACCTCGTCCTACTGCTCAGAGCCTGTTGCCTGCAGCTATGAGGAAAGGGAAGCTTATTCCACGCTTTATTGGGCCATTTGAAGTGTTGGAGCATATTGGCTTCGTACCTTATCGATTGGTTTTACCGCCTTCCCTTTCAGTAGTCCACAATGTCTATCATGTATCCTTGTTATGGAAGTACATGGCGGATCCATCTCATGTCATCGACTACAAGCCATTGTGTTTGAGTGAGAACTTGAGTTATGAAGAGAAGCCAAGACGAATTCTTGCTAGATAA

Coding sequence (CDS)

ATGAGGGTCCTTCTTTTTATAGCCTCGGGCAATCGACCAGGAAGAACGACGGTTAGGGATGGCAGAGACGCGCCACGCTGTACGGGAAGCATCGAGGAGCATGCCTCTAATAGTTGCGGGCCGACACCTCGTCCTACTGCTCAGAGCCTGTTGCCTGCAGCTATGAGGAAAGGGAAGCTTATTCCACGCTTTATTGGGCCATTTGAAGTGTTGGAGCATATTGGCTTCGTACCTTATCGATTGGTTTTACCGCCTTCCCTTTCAGTAGTCCACAATGTCTATCATGTATCCTTGTTATGGAAGTACATGGCGGATCCATCTCATGTCATCGACTACAAGCCATTGTGTTTGAGTGAGAACTTGAGTTATGAAGAGAAGCCAAGACGAATTCTTGCTAGATAA

Protein sequence

MRVLLFIASGNRPGRTTVRDGRDAPRCTGSIEEHASNSCGPTPRPTAQSLLPAAMRKGKLIPRFIGPFEVLEHIGFVPYRLVLPPSLSVVHNVYHVSLLWKYMADPSHVIDYKPLCLSENLSYEEKPRRILAR
Homology
BLAST of Moc05g17140 vs. NCBI nr
Match: XP_038880478.1 (uncharacterized protein LOC120072137 [Benincasa hispida])

HSP 1 Score: 123.6 bits (309), Expect = 1.3e-24
Identity = 58/78 (74.36%), Postives = 67/78 (85.90%), Query Frame = 0

Query: 56  RKGKLIPRFIGPFEVLEHIGFVPYRLVLPPSLSVVHNVYHVSLLWKYMADPSHVIDYKPL 115
           R GKL PRFIGPFEVLE +G V YRL LPP+LS VHNV+HVS+L KY+ADPSHV+DY+PL
Sbjct: 93  RNGKLSPRFIGPFEVLERVGLVAYRLALPPTLSSVHNVFHVSILRKYVADPSHVVDYEPL 152

Query: 116 CLSENLSYEEKPRRILAR 134
            L++NLSYEEKP  ILAR
Sbjct: 153 QLNDNLSYEEKPIEILAR 170

BLAST of Moc05g17140 vs. NCBI nr
Match: TYJ97414.1 (pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 121.3 bits (303), Expect = 6.2e-24
Identity = 55/78 (70.51%), Postives = 68/78 (87.18%), Query Frame = 0

Query: 56  RKGKLIPRFIGPFEVLEHIGFVPYRLVLPPSLSVVHNVYHVSLLWKYMADPSHVIDYKPL 115
           +KGKL PRF+GPFE+LE IG V YRL LPPSLS VH+V+HVS+L KY+ADP+HV+D++PL
Sbjct: 730 KKGKLSPRFVGPFEILERIGLVAYRLALPPSLSTVHDVFHVSMLRKYVADPTHVVDFEPL 789

Query: 116 CLSENLSYEEKPRRILAR 134
            +SENLSYEE+P  ILAR
Sbjct: 790 QISENLSYEEQPVEILAR 807

BLAST of Moc05g17140 vs. NCBI nr
Match: XP_038902486.1 (uncharacterized protein LOC120089142 [Benincasa hispida] >XP_038904308.1 uncharacterized protein LOC120090662 [Benincasa hispida])

HSP 1 Score: 121.3 bits (303), Expect = 6.2e-24
Identity = 56/78 (71.79%), Postives = 67/78 (85.90%), Query Frame = 0

Query: 56  RKGKLIPRFIGPFEVLEHIGFVPYRLVLPPSLSVVHNVYHVSLLWKYMADPSHVIDYKPL 115
           ++GKL PRFIGPFE+LE +G V YRL LPPSLS VHNV+HVS+L KY+AD SHV+DY+P 
Sbjct: 87  KRGKLNPRFIGPFEILERVGLVAYRLALPPSLSAVHNVFHVSMLRKYVADSSHVVDYEPF 146

Query: 116 CLSENLSYEEKPRRILAR 134
            L+ENLSYEE+P RILAR
Sbjct: 147 HLNENLSYEEEPIRILAR 164

BLAST of Moc05g17140 vs. NCBI nr
Match: KAA0067060.1 (pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 120.9 bits (302), Expect = 8.1e-24
Identity = 55/78 (70.51%), Postives = 66/78 (84.62%), Query Frame = 0

Query: 56  RKGKLIPRFIGPFEVLEHIGFVPYRLVLPPSLSVVHNVYHVSLLWKYMADPSHVIDYKPL 115
           R+GKL PRF+GPFE+LE IG V YRL LPPSLS VH+V+HVS+L KY+ADPSHV+DYKPL
Sbjct: 42  RRGKLSPRFVGPFEILERIGLVAYRLALPPSLSTVHDVFHVSMLRKYVADPSHVVDYKPL 101

Query: 116 CLSENLSYEEKPRRILAR 134
            + ENLSY E+P  +LAR
Sbjct: 102 EIDENLSYTEQPVEVLAR 119

BLAST of Moc05g17140 vs. NCBI nr
Match: KAA0059580.1 (pol protein [Cucumis melo var. makuwa] >TYK12221.1 pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 120.6 bits (301), Expect = 1.1e-23
Identity = 53/78 (67.95%), Postives = 66/78 (84.62%), Query Frame = 0

Query: 56   RKGKLIPRFIGPFEVLEHIGFVPYRLVLPPSLSVVHNVYHVSLLWKYMADPSHVIDYKPL 115
            R+GKL PRF+GPFE+LE  G V YRL LPPSLS VH+V+HVS+LWKY++DPSHV+DY+PL
Sbjct: 947  RRGKLSPRFVGPFEILERTGPVAYRLALPPSLSAVHDVFHVSMLWKYVSDPSHVVDYEPL 1006

Query: 116  CLSENLSYEEKPRRILAR 134
             + ENLSY E+P  +LAR
Sbjct: 1007 EIDENLSYTERPVEVLAR 1024

BLAST of Moc05g17140 vs. ExPASy TrEMBL
Match: A0A5D3BEF2 (Pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G002390 PE=4 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 3.0e-24
Identity = 55/78 (70.51%), Postives = 68/78 (87.18%), Query Frame = 0

Query: 56  RKGKLIPRFIGPFEVLEHIGFVPYRLVLPPSLSVVHNVYHVSLLWKYMADPSHVIDYKPL 115
           +KGKL PRF+GPFE+LE IG V YRL LPPSLS VH+V+HVS+L KY+ADP+HV+D++PL
Sbjct: 730 KKGKLSPRFVGPFEILERIGLVAYRLALPPSLSTVHDVFHVSMLRKYVADPTHVVDFEPL 789

Query: 116 CLSENLSYEEKPRRILAR 134
            +SENLSYEE+P  ILAR
Sbjct: 790 QISENLSYEEQPVEILAR 807

BLAST of Moc05g17140 vs. ExPASy TrEMBL
Match: A0A5A7VIM5 (Pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold38G001090 PE=4 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 3.9e-24
Identity = 55/78 (70.51%), Postives = 66/78 (84.62%), Query Frame = 0

Query: 56  RKGKLIPRFIGPFEVLEHIGFVPYRLVLPPSLSVVHNVYHVSLLWKYMADPSHVIDYKPL 115
           R+GKL PRF+GPFE+LE IG V YRL LPPSLS VH+V+HVS+L KY+ADPSHV+DYKPL
Sbjct: 42  RRGKLSPRFVGPFEILERIGLVAYRLALPPSLSTVHDVFHVSMLRKYVADPSHVVDYKPL 101

Query: 116 CLSENLSYEEKPRRILAR 134
            + ENLSY E+P  +LAR
Sbjct: 102 EIDENLSYTEQPVEVLAR 119

BLAST of Moc05g17140 vs. ExPASy TrEMBL
Match: A0A5D3CK76 (Pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold769G00250 PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 5.1e-24
Identity = 53/78 (67.95%), Postives = 66/78 (84.62%), Query Frame = 0

Query: 56   RKGKLIPRFIGPFEVLEHIGFVPYRLVLPPSLSVVHNVYHVSLLWKYMADPSHVIDYKPL 115
            R+GKL PRF+GPFE+LE  G V YRL LPPSLS VH+V+HVS+LWKY++DPSHV+DY+PL
Sbjct: 947  RRGKLSPRFVGPFEILERTGPVAYRLALPPSLSAVHDVFHVSMLWKYVSDPSHVVDYEPL 1006

Query: 116  CLSENLSYEEKPRRILAR 134
             + ENLSY E+P  +LAR
Sbjct: 1007 EIDENLSYTERPVEVLAR 1024

BLAST of Moc05g17140 vs. ExPASy TrEMBL
Match: A0A5A7UCD5 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold428G00810 PE=4 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 2.0e-23
Identity = 54/78 (69.23%), Postives = 66/78 (84.62%), Query Frame = 0

Query: 56   RKGKLIPRFIGPFEVLEHIGFVPYRLVLPPSLSVVHNVYHVSLLWKYMADPSHVIDYKPL 115
            R+GKL PRF+GPFE+LE IG V YRL LPPSLS VHNV+HVS+L KY++DPSHV+DY+PL
Sbjct: 1311 RRGKLSPRFVGPFEILERIGPVAYRLALPPSLSTVHNVFHVSMLRKYVSDPSHVVDYEPL 1370

Query: 116  CLSENLSYEEKPRRILAR 134
             + ENLSY E+P  +LAR
Sbjct: 1371 EIDENLSYTEQPIEVLAR 1388

BLAST of Moc05g17140 vs. ExPASy TrEMBL
Match: A0A5A7V810 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold329G002410 PE=4 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 2.0e-23
Identity = 55/78 (70.51%), Postives = 65/78 (83.33%), Query Frame = 0

Query: 56   RKGKLIPRFIGPFEVLEHIGFVPYRLVLPPSLSVVHNVYHVSLLWKYMADPSHVIDYKPL 115
            RKGKL PRF+GPFE+LE IG V YRL LPPSLS VH+V+HVS+L KY+ DPSHV+DYKPL
Sbjct: 1796 RKGKLSPRFVGPFEILERIGPVAYRLALPPSLSTVHDVFHVSMLRKYVPDPSHVVDYKPL 1855

Query: 116  CLSENLSYEEKPRRILAR 134
             + ENLSY E+P  +LAR
Sbjct: 1856 EIDENLSYTEQPFEVLAR 1873

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038880478.11.3e-2474.36uncharacterized protein LOC120072137 [Benincasa hispida][more]
TYJ97414.16.2e-2470.51pol protein [Cucumis melo var. makuwa][more]
XP_038902486.16.2e-2471.79uncharacterized protein LOC120089142 [Benincasa hispida] >XP_038904308.1 unchara... [more]
KAA0067060.18.1e-2470.51pol protein [Cucumis melo var. makuwa][more]
KAA0059580.11.1e-2367.95pol protein [Cucumis melo var. makuwa] >TYK12221.1 pol protein [Cucumis melo var... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3BEF23.0e-2470.51Pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G002390 P... [more]
A0A5A7VIM53.9e-2470.51Pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold38G001090 PE... [more]
A0A5D3CK765.1e-2467.95Pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold769G00250 PE... [more]
A0A5A7UCD52.0e-2369.23Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold42... [more]
A0A5A7V8102.0e-2370.51Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold32... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR46148:SF21DOMAIN PROTEIN, PUTATIVE-RELATEDcoord: 55..131
NoneNo IPR availablePANTHERPTHR46148FAMILY NOT NAMEDcoord: 55..131

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc05g17140.1Moc05g17140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006508 proteolysis
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
molecular_function GO:0004190 aspartic-type endopeptidase activity
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003964 RNA-directed DNA polymerase activity
molecular_function GO:0008270 zinc ion binding