Lsi01G003010 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G003010
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	RING-type domain-containing protein
Location	chr01: 2689575 .. 2694329 (-)
RNA-Seq Expression	Lsi01G003010
Synteny	Lsi01G003010

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTGGTGTGTTTTTCTTTAATCAATCTAATGCCGTGGAAATTTCGTAAAACCACGAACACGAGGCTCCTTCCCTCTCGGCTCTGATATTTTGCTCCACCGCCCCCTTCATCATCTCTACGAACCTCAATTTTTGTGAACTTACAACTTCTTTTCGTTGTTTCTTCAACTTATTTTGCTTCAGACCGAAGCAATGCCGGCTCGGAAGCGCGCTTACGAGACTGTGAATGTTGATCTTTTGCCGCGACTCCATAATACCTTCAATGAATGCTCTGACGATGGAGAATCTGAGGGTACGCTTCTTGTTTCTCTTCAGCACCCTAAATTTTCACGATGCTACCGACTCTTTGGTGTTCCTGCATTTCATATATGTTATTATTTGTTTGTTTAATTCTTTTTTTTGTGCGTCTACTGAGTTCGTGGTGTTCTATGTTGCTCCTTCTTTCGCCAGAATCCGATCGAAGCCCTTCGTCCAGTCAAGGAGATAAGGACGAGTATGTTCCGTTTATGAACTCTGTTTTTCGCTTGCTTCTATTTGTTCGTCATCTTCTTCGTGCTCTTCCTTTTTACTCTATCTACTCTTTTGATTTATTTCCTCTGGTTGGTTATGGAGTTCTTTGGTGCTTAACTGCTTGGGATTGATTTGGGATCTGCATGGTATGCTTCGTGGTATTGTTCATCGAGGTCTTGCTGCGACCGTATTCGAAGACCTGCTGTGTGGGCTTCACGTGCTGAAGCATTCGTCGATTTTTGTCCTGCACCGGAGTTTTCTTTACTTTCAAGTTTTGAGTTTCTTTACCGATTATGGTTAGTGGAATTGGGTGCTGGATCTTGTGGGCGAAGTGGGTTCTCGAGGTCGTATATCCGATCAATCTGCTTTTACATATGCTACGAGCTGCTGGGTGGGTAATGACGTTGGTGCTCACATGGTTTTAGCTTCTTTACGATATTAAAGCTTACATGATCCGAGATCAAGCTTTCACTCAACGTGAAATCAAGCTTATTATAACTGAAGCGTTCACGGAAAAATCTTGCTGGTTCCCGCCATTGATTGTCGGATCCAATTTTACGCAAATTCCAAGAAGTTAACGACTGGAAACCCGACATTTCATTAAGAACGATTTTACCCAAATTTTTTACAACTTGAGCGGTACTCTTACGCTAGCGCGATGAACGAACTATTTGGGTTGCGTTATAGAATATATGTATCTGAACAGAAAGTTGTTTATCGGTTCTTTAGTTGCATGGTGAACAGTAGAATTCCCTGAACGATTAACTCGATCTTAGGTGTCTTGCGATTATTCCGACATCCTAAATCTTCTGTCTGGTTTTCTTTTCCAGATTTATCGCCGTGAAATTGTCAGACATTCGGAGAGAAGTTCAATGTCCTATCTGCTTAGGTATGTTTTAATAATCTCTTCTTGTTGAACGTATTTATCTCATATAAGATAAGTTTTCTATTGATTTGAACATTTGGTTTTAATTCTTATACAATTCATGTTCTGCAAGTATATAAACACGCCTACTACTTGAAACGACTTTGTGTTTACCATTGGTGTCAATGTGTCATCCAGGTCTTTACTGACTTTAACAAGTAAGATTGTTCTCATATCCTGAGAATCAATCATCGTGTATAATTAACTATTTTCGTAAGTTTATGTACATACGCACTGCAAGATGACCTATCAATAAGGTTATTTCAAATGATTGATTTGTCCGGGATGAATTGTGGAGGATGAGAGCATTAATTTTAAATGAAGTGTTGAATCAAGTGCCACCGGACTCATCTGATCCAAGAATTTATTCTGATATAACAAATTTGATTTTCTGTATATAAGTTGTATCAAATAACATAAAGATTGTTTGTGATCTCCGGATCTGGGAGTGATGGCAATCATTAAAATCTGAGGTTGACTTTGTATATGGACTATCGATTTAGATATGGATTCTGATATGGCTTCTTTATCAGTTTTTGCTTTGGCTTAAAAAAGTTATTAGTCAGTTGTCACCCCCCCTCCACTTCCAGGGTCTTGCATATAAAACAATTGCATTTTTACGTTCCATGGACCTCTGTTTGTATTTTAGGTGACACTTATAATTGCAATCCTTCTGCTTTTCTTATTCCCCACAAGTAACTTAGAAGTTAAGATGACAAACTTTTCTACCTCCACTTATTTGTCCTAACATCAACTTCGTTATCGAGTTTTGTATTTTTTGATTAGACCATTCCGTTAAAGTAACTAATTGGACCGTAGAGTGTGAATTCATGAGCATATGAGGGTATGGAATTTGGTGATCTGCATTCTTTTTCGAACCTTACCGTTTCAGCCTTTCAGGCTACATAAGTTTATGTGGAGCCAGTAATTTATGATGGTGTTACATGTTTACACATAACAAGTAGTAAATATATGCATGACTTTCAAATTATTGATTACTCAAGCTCTAATTGTCAAGATTATTGATCCTTACTTTCAGTTCATGCAGTATCTGATAAAGGAAACACCCAAAAATCTTCCTTATCATAAGACAATAACTAAATTCCAACTCGAAACATTCCTGAAATTTTACATTTTTTAGTGGATGTATCCAAATACTATGAGCCATGACATGCTAAGTCTTAGCATGTTTGGGAAGTATATTTAGGTGGATTTGGTGAGGCAACCTCAGAGTTCACTTTGGAAGGTCATCTCCATGTTTATGCATGAGTAGTATTCTTAAGGAGGTTGATAAAGTTTTTTCAATTCCTAATGTGCAGGAATCATCAGAAAAACCAGAACAGTGATGGAATGTCTGCACCGCTTCTGCAGGGAATGCATAGACAAATCGATGCGTCTAGGGTATGTTCTTATGTAATTTTTTATTTTGATCTTGCTAATTGCTATACAGCTCAAGAAAATAAATATATATCGCAGCACAACTTTCCTTCTGGCTTCCACTAAACTTTTACTGAAATTTGCTGCAGTAACAATGAGTGCCCTGCTTGCCGCACTCATTGTGCCAGTCGACGCTCTCTGAGAGATGATCCGAATTATGATACATTGATTGCTGTGTTATATCCAGATATTGACAAATATGAGGAAGAGGTGGGAATATTATGTTTAAGCTTGCAAGCTTGGTGTTCGATGATTGATATCGTCATTTCTGCACACTCCACTATTGTTAATTTTTTTTTTTTTTTTTTTTTTGGGAACTGGCTTTCCAAGAAGAAGAGAAAGCTCGTAATAAGCAGGTGAGAAGGCAAAGTTCCGTGCATTTTTTTCGTTATTGACATGAATTTGTTTATTTTCCAGAGTTGATGAGCTCACTTTACATTGTTCGATTACAAGAAACCACAGATTTTGTCATGCTAAAATTGATTGAGAGAGAGAGAGAGAGGAATTATTTAAATCATTATTTGATAAATATGTAATGCAAAATTGTGGGAGCATTGGGACATCAAATAGAGCATGTTTTTCTCAGCTGTTGTGCTTTTCCATTCCATTTTACTTGGGAACATCTCGAGCTGAAAACCAACATGGCTATTGTAGATTGTACATGTATCAGGTATCTATGATGACACTTGATCCTTATTCTTCATACTTGGTCTAAAATGTTACTTTACGGTTATTTTGCAGATTCAAACTTCCATTGCTCAGACACTTCAACGACAAACTGAAGCTTTGGGTAGAAAACGTTCTAAACCTTCGAGGAGATTAAGTTCGAGAGGACGCAAAAGCTTCCAAAATCATATTGAGAGTCTATGCTTCGATGAAAATGAGGACGAAAATGATTATGATGTCAGCAAAAATTCATCTTCTGCTGATGAGCGCATGGACACCAGACCTAAAAGATCCAGGAGAGGTGGTCCTGTTAGATTTTCCCAGTCTTCTTCAGCTACTGGAGCAGATGGAGCAGATGGTGGTGGTATCGAAAATGATTATGAAGTGAACAAAGAAAAAGTTGGTGCATCTATAGGGTTTGTTGGTAGCTCAGAAAAACTTTCCTGGGGCAAAGGTGGAATAAGAAGTCACACTAGATATGGGGGTATAAATGGGGGGGCTGGTAAAAATTCCAGGAATAACCGCATCATGAAGCTGTCGGATTATGTTCGAAACTCAGAAAATGTCAGTGAGGAGGAGGTAAAAACAACAATTTGAGGTGTTACTAAACTTCCTAATGAATAAACTGTGGACATGTTAACGTATAAATGGCTCGCCTATTGAATCACATGCATGCTGTTATGCCTCTTTTAGCTGCTTAAAAGCTCAACGATCTTTTATGTTTCTATCCATCAATGACTTTTAACTTCTCTTCCTGACTTTTAGTTGGACATCCACCTTGTGCTCGTTTCCATGGATGAAACTAGAATACCCGCATTGCAAAGACCATATATTTGCTGCAGGCCTTCAGTGACGATTGGACACCTAAGTCAAGTAATCTGTCATTAAATGGAGCTGCACTTCATCCGTTTAAGGTCTTATTCATTTATTTAGTTGATTCTTGAGCTTCCTTTCTTCTGCAGTATGTCGCTCTCGAAACCGCGCTTCCCGTTGATGTTGTTGAAATGTGCATGGCGAAAGAGCTGCAAATGAAACTCAATCCTTCAACCTCCGGAACTCTAAAGAACCCATGTAAAGAAAGTATACAGATACTAAACGAGCAAGAAACGCTATCGACAGCCAAACTTAAAGCACACAGGCTAGCTTGTGGCTATCTGGTATGCCCTGAATCTACAGCACACAAATGCTTGAAAAAAATGCACCCACTACATCAATAA

mRNA sequence

CTTTGGTGTGTTTTTCTTTAATCAATCTAATGCCGTGGAAATTTCGTAAAACCACGAACACGAGGCTCCTTCCCTCTCGGCTCTGATATTTTGCTCCACCGCCCCCTTCATCATCTCTACGAACCTCAATTTTTGTGAACTTACAACTTCTTTTCGTTGTTTCTTCAACTTATTTTGCTTCAGACCGAAGCAATGCCGGCTCGGAAGCGCGCTTACGAGACTGTGAATGTTGATCTTTTGCCGCGACTCCATAATACCTTCAATGAATGCTCTGACGATGGAGAATCTGAGGAATCCGATCGAAGCCCTTCGTCCAGTCAAGGAGATAAGGACGAGTCTTGCTGCGACCGTATTCGAAGACCTGCTGTGTGGGCTTCACGTGCTGAAGCATTCGTCGATTTTTGTCCTGCACCGGAGTTTTCTTTACTTTCAAGTTTTGAGTTTCTTTACCGATTATGGTTAGTGGAATTGGGTGCTGGATCTTGTGGGCGAAGTGGGTTCTCGAGATTTATCGCCGTGAAATTGTCAGACATTCGGAGAGAAGTTCAATGTCCTATCTGCTTAGGAATCATCAGAAAAACCAGAACAGTGATGGAATGTCTGCACCGCTTCTGCAGGGAATGCATAGACAAATCGATGCGTCTAGGTAACAATGAGTGCCCTGCTTGCCGCACTCATTGTGCCAGTCGACGCTCTCTGAGAGATGATCCGAATTATGATACATTGATTGCTGTGTTATATCCAGATATTGACAAATATGAGGAAGAGATTCAAACTTCCATTGCTCAGACACTTCAACGACAAACTGAAGCTTTGGGTAGAAAACGTTCTAAACCTTCGAGGAGATTAAGTTCGAGAGGACGCAAAAGCTTCCAAAATCATATTGAGAGTCTATGCTTCGATGAAAATGAGGACGAAAATGATTATGATGTCAGCAAAAATTCATCTTCTGCTGATGAGCGCATGGACACCAGACCTAAAAGATCCAGGAGAGGTGGTCCTGTTAGATTTTCCCAGTCTTCTTCAGCTACTGGAGCAGATGGAGCAGATGGTGGTGGTATCGAAAATGATTATGAAGTGAACAAAGAAAAAGTTGGTGCATCTATAGGGTTTGTTGGTAGCTCAGAAAAACTTTCCTGGGGCAAAGGTGGAATAAGAAGTCACACTAGATATGGGGGTATAAATGGGGGGGCTGGTAAAAATTCCAGGAATAACCGCATCATGAAGCTGTCGGATTATGTTCGAAACTCAGAAAATGTCAGTGAGGAGGAGTTGGACATCCACCTTGTGCTCGTTTCCATGGATGAAACTAGAATACCCGCATTGCAAAGACCATATATTTGCTGCAGGCCTTCAGTGACGATTGGACACCTAAGTCAATATGTCGCTCTCGAAACCGCGCTTCCCGTTGATGTTGTTGAAATGTGCATGGCGAAAGAGCTGCAAATGAAACTCAATCCTTCAACCTCCGGAACTCTAAAGAACCCATGTAAAGAAAGTATACAGATACTAAACGAGCAAGAAACGCTATCGACAGCCAAACTTAAAGCACACAGGCTAGCTTGTGGCTATCTGGTATGCCCTGAATCTACAGCACACAAATGCTTGAAAAAAATGCACCCACTACATCAATAA

Coding sequence (CDS)

ATGCCGGCTCGGAAGCGCGCTTACGAGACTGTGAATGTTGATCTTTTGCCGCGACTCCATAATACCTTCAATGAATGCTCTGACGATGGAGAATCTGAGGAATCCGATCGAAGCCCTTCGTCCAGTCAAGGAGATAAGGACGAGTCTTGCTGCGACCGTATTCGAAGACCTGCTGTGTGGGCTTCACGTGCTGAAGCATTCGTCGATTTTTGTCCTGCACCGGAGTTTTCTTTACTTTCAAGTTTTGAGTTTCTTTACCGATTATGGTTAGTGGAATTGGGTGCTGGATCTTGTGGGCGAAGTGGGTTCTCGAGATTTATCGCCGTGAAATTGTCAGACATTCGGAGAGAAGTTCAATGTCCTATCTGCTTAGGAATCATCAGAAAAACCAGAACAGTGATGGAATGTCTGCACCGCTTCTGCAGGGAATGCATAGACAAATCGATGCGTCTAGGTAACAATGAGTGCCCTGCTTGCCGCACTCATTGTGCCAGTCGACGCTCTCTGAGAGATGATCCGAATTATGATACATTGATTGCTGTGTTATATCCAGATATTGACAAATATGAGGAAGAGATTCAAACTTCCATTGCTCAGACACTTCAACGACAAACTGAAGCTTTGGGTAGAAAACGTTCTAAACCTTCGAGGAGATTAAGTTCGAGAGGACGCAAAAGCTTCCAAAATCATATTGAGAGTCTATGCTTCGATGAAAATGAGGACGAAAATGATTATGATGTCAGCAAAAATTCATCTTCTGCTGATGAGCGCATGGACACCAGACCTAAAAGATCCAGGAGAGGTGGTCCTGTTAGATTTTCCCAGTCTTCTTCAGCTACTGGAGCAGATGGAGCAGATGGTGGTGGTATCGAAAATGATTATGAAGTGAACAAAGAAAAAGTTGGTGCATCTATAGGGTTTGTTGGTAGCTCAGAAAAACTTTCCTGGGGCAAAGGTGGAATAAGAAGTCACACTAGATATGGGGGTATAAATGGGGGGGCTGGTAAAAATTCCAGGAATAACCGCATCATGAAGCTGTCGGATTATGTTCGAAACTCAGAAAATGTCAGTGAGGAGGAGTTGGACATCCACCTTGTGCTCGTTTCCATGGATGAAACTAGAATACCCGCATTGCAAAGACCATATATTTGCTGCAGGCCTTCAGTGACGATTGGACACCTAAGTCAATATGTCGCTCTCGAAACCGCGCTTCCCGTTGATGTTGTTGAAATGTGCATGGCGAAAGAGCTGCAAATGAAACTCAATCCTTCAACCTCCGGAACTCTAAAGAACCCATGTAAAGAAAGTATACAGATACTAAACGAGCAAGAAACGCTATCGACAGCCAAACTTAAAGCACACAGGCTAGCTTGTGGCTATCTGGTATGCCCTGAATCTACAGCACACAAATGCTTGAAAAAAATGCACCCACTACATCAATAA

Protein sequence

MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVWASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEEIQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLVCPESTAHKCLKKMHPLHQ

Homology

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BLAST of Lsi01G003010 vs. ExPASy Swiss-Prot
Match: Q9FKW0 (Putative E3 ubiquitin-protein ligase RING1a OS=Arabidopsis thaliana OX=3702 GN=RING1A PE=1 SV=2)

HSP 1 Score: 283.9 bits (725), Expect = 3.4e-75
Identity = 171/383 (44.65%), Postives = 237/383 (61.88%), Query Frame = 0


Query: 104 SRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHC 163
           S F+ + L +IR++VQCPICLGII+KTRTVMECLHRFCRECIDKSMRLGNNECPACR HC
Sbjct: 120 SEFMEIDLGEIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRKHC 179

Query: 164 ASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQTLQRQTEALG 223
           ASRRSLRDDP +D LIA L+ +ID YEEE              IQ SIAQ  QRQ+EAL 
Sbjct: 180 ASRRSLRDDPKFDALIAALFTNIDSYEEEELAFHEDEMARNKQIQASIAQISQRQSEALV 239

Query: 224 RKRS---------------KPSRRLSSRGRKSFQNHIESLCFDENEDENDYDVSKNSSSA 283
           ++RS                 SRR  +      QN  E+   D+N+D N+    K+SSS 
Sbjct: 240 KRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQQNASEAHEDDDNDDNNNRGRDKDSSSD 299

Query: 284 DERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGSSEKL 343
           +   + R K+ R+    R +Q  S++GA+  +G   +ND EV ++  G S G V + E L
Sbjct: 300 ERGTEVRQKKRRKRSTSRSTQHPSSSGANKNNGNCADNDTEVYRDTKGISPGLVWNPEIL 359

Query: 344 SWGKGGIRSHTRYG-GINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSMDET 403
           +WG+GG RS+TR+G   +GG+ K+ RN R+ KL +Y+R+S + S  ELDIH+ LVS+D  
Sbjct: 360 AWGRGGTRSNTRHGNNTSGGSSKSVRNARVNKLVEYLRSSVDGSSVELDIHVKLVSLDTK 419

Query: 404 RIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAK-----ELQMKLNPSTSGT 452
            IP L +PY+CCRP++ +  L ++VAL+  L  + VE+ + +     +  ++  P+ +  
Sbjct: 420 CIPDLPQPYLCCRPTLLVKQLREFVALQIHLKTEEVELLVTRRRVGEDAAIENLPAVTPA 479

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BLAST of Lsi01G003010 vs. ExPASy Swiss-Prot
Match: Q0WX00 (Putative E3 ubiquitin-protein ligase RING1b OS=Arabidopsis thaliana OX=3702 GN=RING1B PE=1 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.8e-63
Identity = 168/393 (42.75%), Postives = 230/393 (58.52%), Query Frame = 0


Query: 95  AGSCGRSGFSRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 154
           + S G    S ++ V L+DI ++VQC ICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN
Sbjct: 78  SSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 137

Query: 155 ECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQT 214
           ECP CR HCASRRSLRDDPN+D LIA L+ +IDK+EEE              IQ SIAQ 
Sbjct: 138 ECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQIQASIAQV 197

Query: 215 LQRQTEALGRKRS--------KPSRRL--SSRGRKSFQNHIESLCFDENEDENDYDVSKN 274
            QRQ++AL +++S          SRR    SR R++ +N IE    + N+D++     K+
Sbjct: 198 SQRQSKALVKRKSVGKGTAILSRSRRSGGGSRRRRNCRN-IEQDTSEANDDDDQNKRGKD 257

Query: 275 SSSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGS 334
           SSS +     R KRS          SS+A   D   G G E  ++ +   +   +  V +
Sbjct: 258 SSSDEPCERQRKKRS-----ATQPSSSNANNNDNCAGNGTEQTHQRDSRVISPVL--VWN 317

Query: 335 SEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSM 394
           SE ++WG+GG RS+TR G  N GA  + RN R+ +L +Y+ + E  +  ELDIHL LVS+
Sbjct: 318 SELIAWGRGGTRSNTRQGNNNQGA-ISKRNARLKRLVEYLGSLEG-NSVELDIHLKLVSL 377

Query: 395 DETRIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAKELQMKLNPSTSGTLK 454
           D   +  L  PY+C RP++ +  L +YVA    L  + VE+ ++K+    +   TS    
Sbjct: 378 DTEGLLNLHEPYLCFRPTLLVKQLREYVARHLKLKAEEVELLVSKDGDTVIGNKTS---- 437

Query: 455 NPCKESIQILNEQETLSTAKLKAHRLAC-GYLV 463
               E +Q L + ET+  AKLK   ++  GY++
Sbjct: 438 ---TEKMQSLQDDETV--AKLKVDCISSNGYMI 451

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BLAST of Lsi01G003010 vs. ExPASy Swiss-Prot
Match: Q5TJF3 (E3 ubiquitin-protein ligase RING1 OS=Canis lupus familiaris OX=9615 GN=RING1 PE=3 SV=2)

HSP 1 Score: 99.8 bits (247), Expect = 9.2e-20
Identity = 66/197 (33.50%), Postives = 94/197 (47.72%), Query Frame = 0


Query: 107 IAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASR 166
           IAV    +  E+ CPICL +++ T T  ECLHRFC +CI  ++R GN ECP CR    S+
Sbjct: 35  IAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSK 94

Query: 167 RSLRDDPNYDTLIAVLYPDIDKYEEE-----IQTSIAQTLQRQTEALGRKRSKPSRRLSS 226
           RSLR DPN+D LI+ +YP  ++YE       I+ S     Q  + ++       +   + 
Sbjct: 95  RSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQ 154

Query: 227 RGRKSFQNHIESLCF-----DENEDENDYDVSKNSSSADERMDTRPKRSRRGGPVRFSQS 286
           R R+      ++        +  E E D +   + S+ D      PKR R GG    S  
Sbjct: 155 RVRRPMPGSDQTTTMSGGEGEPGEGEGDGEDVSSDSAPDSAPGPAPKRPRGGGAGGSSVG 214

Query: 287 SSATGADGADGGGIEND 294
           +   GA G  GG    D
Sbjct: 215 TGGGGAGGVGGGAGSED 231

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BLAST of Lsi01G003010 vs. ExPASy Swiss-Prot
Match: A1YER5 (E3 ubiquitin-protein ligase RING1 OS=Gorilla gorilla gorilla OX=9595 GN=RING1 PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 9.2e-20
Identity = 66/197 (33.50%), Postives = 93/197 (47.21%), Query Frame = 0


Query: 107 IAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASR 166
           IAV    +  E+ CPICL +++ T T  ECLHRFC +CI  ++R GN ECP CR    S+
Sbjct: 6   IAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSK 65

Query: 167 RSLRDDPNYDTLIAVLYPDIDKYEEE-----IQTSIAQTLQRQTEALGRKRSKPSRRLSS 226
           RSLR DPN+D LI+ +YP  ++YE       I+ S     Q  + ++       +   + 
Sbjct: 66  RSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQ 125

Query: 227 RGRKSFQNHIESLCF-----DENEDENDYDVSKNSSSADERMDTRPKRSRRGGPVRFSQS 286
           R R+      ++        D  E E D +   + S+ D      PKR R GG    S  
Sbjct: 126 RVRRPIPGSDQTTTMSGGEGDPGEGEGDGEDVSSDSAPDSAPGPAPKRPRGGGAGGSSVG 185

Query: 287 SSATGADGADGGGIEND 294
           +   G  G  GG    D
Sbjct: 186 TGGGGTGGVGGGAGSED 202

[Close]

BLAST of Lsi01G003010 vs. ExPASy Swiss-Prot
Match: Q06587 (E3 ubiquitin-protein ligase RING1 OS=Homo sapiens OX=9606 GN=RING1 PE=1 SV=2)

HSP 1 Score: 98.2 bits (243), Expect = 2.7e-19
Identity = 65/197 (32.99%), Postives = 93/197 (47.21%), Query Frame = 0


Query: 107 IAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASR 166
           IAV    +  E+ CPICL +++ T T  ECLHRFC +CI  ++R GN ECP CR    S+
Sbjct: 35  IAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSK 94

Query: 167 RSLRDDPNYDTLIAVLYPDIDKYEEE-----IQTSIAQTLQRQTEALGRKRSKPSRRLSS 226
           RSLR DPN+D LI+ +YP  ++YE       I+ S     Q  + ++       +   + 
Sbjct: 95  RSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQ 154

Query: 227 RGRKSFQNHIESLCF-----DENEDENDYDVSKNSSSADERMDTRPKRSRRGGPVRFSQS 286
           R R+      ++        +  E E D +   + S+ D      PKR R GG    S  
Sbjct: 155 RVRRPIPGSDQTTTMSGGEGEPGEGEGDGEDVSSDSAPDSAPGPAPKRPRGGGAGGSSVG 214

Query: 287 SSATGADGADGGGIEND 294
           +   G  G  GG    D
Sbjct: 215 TGGGGTGGVGGGAGSED 231

[Close]

BLAST of Lsi01G003010 vs. ExPASy TrEMBL
Match: A0A6J1EFU8 (putative E3 ubiquitin-protein ligase RING1a isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433005 PE=4 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 2.5e-190
Identity = 366/493 (74.24%), Postives = 388/493 (78.70%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVW 60
           MPARKR YETV+ DLLPRLH+TFN+ S+DGESEESDRS SSSQ DKDE            
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSSQEDKDE------------ 60

Query: 61  ASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQC 120
                                                        F+AVKLSDIRR+VQC
Sbjct: 61  ---------------------------------------------FVAVKLSDIRRDVQC 120

Query: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIA 180
           PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIA
Sbjct: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIA 180

Query: 181 VLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKS 240
           VLYPDIDKYEEE              IQ+SIAQTL+RQTEALGRKRSKP RRLSSRGRKS
Sbjct: 181 VLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGRKS 240

Query: 241 FQNHIESLCFDENEDENDYDVSKNSSSADERMDTRP-KRSRRGGPVRFSQSSSATGADGA 300
           FQNHIESL FDENEDENDYD SKNSSSADERMDTRP KRSRRGGPVRFSQSSSATGADGA
Sbjct: 241 FQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGADGA 300

Query: 301 DGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMK 360
           DGG  ENDYEVNKE+VGAS+G V SSEKLSWGKGGIRSHTRYGGINGG GK SRNNRI K
Sbjct: 301 DGGSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNNRITK 360

Query: 361 LSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETALP 420
           LSDYVRNSEN+SEEELD+ L+LVSMD+TRIPAL+  YICCRPSVTIGHL Q VALETAL 
Sbjct: 361 LSDYVRNSENISEEELDVQLMLVSMDKTRIPALRSSYICCRPSVTIGHLRQLVALETALS 420

Query: 421 VDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLVCPE 479
           VDV+EM + KELQMK+NPSTSGT  +PCKES QILN+QETLS  KLK HRLACGYLVC E
Sbjct: 421 VDVIEMYVVKELQMKINPSTSGTFLDPCKESAQILNQQETLSMDKLKTHRLACGYLVCLE 436

[Close]

BLAST of Lsi01G003010 vs. ExPASy TrEMBL
Match: A0A5D3CNZ3 (Putative E3 ubiquitin-protein ligase RING1a OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007610 PE=4 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 2.4e-188
Identity = 377/511 (73.78%), Postives = 405/511 (79.26%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLL-PRLHNTF-NECSDDGESEESDRSPSSSQGDKDE--SCCDRIRR 60
           MPARKRAY++++VDLL PRLHNTF NE S D +S+ESDRS SSSQGD+DE       +  
Sbjct: 1   MPARKRAYDSLDVDLLPPRLHNTFNNERSQDEQSQESDRSSSSSQGDEDEYVPFMSSVLS 60

Query: 61  PAVWASRAEAFVDFCPAPEFSLLSSFEF--------------LYRLWLVELGAGSCGRSG 120
             ++       V F     F L     +              L+ +  VELGAGSCGRS 
Sbjct: 61  LLLFVRLLFRVVPFYSIYSFDLFRLVGYGVSFVANCLGLIWDLHGVPRVELGAGSCGRSE 120

Query: 121 FSR-----------------FIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECI 180
           FSR                 FIAVKLSDIR+EVQCPICLGII+KTRTVMECLHRFCRECI
Sbjct: 121 FSRLYVRSICFYICYELLGGFIAVKLSDIRKEVQCPICLGIIKKTRTVMECLHRFCRECI 180

Query: 181 DKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE------------- 240
           DKSMRLGN ECPACRTHCASRRSLRDDPNYDTLIAVLYPDI+KYEEE             
Sbjct: 181 DKSMRLGNKECPACRTHCASRRSLRDDPNYDTLIAVLYPDIEKYEEEELAFQEEEKARNK 240

Query: 241 -IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKSFQNHIESLCFDENEDENDYDVSKNS 300
            IQTSIAQTLQRQTE+LGRKRSKPSRRLSSRG KSFQNHIESLCFDENEDENDYDVSKNS
Sbjct: 241 QIQTSIAQTLQRQTESLGRKRSKPSRRLSSRGPKSFQNHIESLCFDENEDENDYDVSKNS 300

Query: 301 SSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGSS 360
           SSADERMDTRPKR RRGGPVRFSQSSSATGADGAD GGIE+DY      VGAS+G VGSS
Sbjct: 301 SSADERMDTRPKRPRRGGPVRFSQSSSATGADGADVGGIEHDY------VGASLGLVGSS 360

Query: 361 EKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSMD 420
           EKLSWGKGGIRSHTRYGG NGGAGKNSRNNRI KLSDYVRNSENVSEEELDIH++LVSMD
Sbjct: 361 EKLSWGKGGIRSHTRYGGTNGGAGKNSRNNRIAKLSDYVRNSENVSEEELDIHVLLVSMD 420

Query: 421 ETRIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAKELQMKLNPSTSGTLKN 463
            + IPALQRPYICCRPSV IGHLSQYVALETAL VDVVE+C+A ELQMKL+PSTS  +KN
Sbjct: 421 RS-IPALQRPYICCRPSVMIGHLSQYVALETALSVDVVEICVANELQMKLDPSTSEAIKN 480

[Close]

BLAST of Lsi01G003010 vs. ExPASy TrEMBL
Match: A0A0A0KLI2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517990 PE=4 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 8.5e-186
Identity = 363/478 (75.94%), Postives = 386/478 (80.75%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLL-PRLHNTF-NECSDDGESEESDRSPSSSQGDKDESCCDRIRRPA 60
           MPARKRAY++++VDLL PRLHNTF NE S D +S+ESDRS SSSQGD+DE          
Sbjct: 1   MPARKRAYDSLDVDLLPPRLHNTFNNERSQDEQSQESDRSSSSSQGDEDE---------- 60

Query: 61  VWASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREV 120
                                                          FIAVKLSDIR+EV
Sbjct: 61  -----------------------------------------------FIAVKLSDIRKEV 120

Query: 121 QCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTL 180
           QCPICLGII+KTRTVMECLHRFCRECIDKSMRLGN ECPACRTHCASRRSLRDDPNYDTL
Sbjct: 121 QCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNYDTL 180

Query: 181 IAVLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGR 240
           IAVLYPDI+KYEEE              IQTSIAQTLQRQTE+LGRKRSKPSRRLSSRG 
Sbjct: 181 IAVLYPDIEKYEEEELAFQEEEKARNKQIQTSIAQTLQRQTESLGRKRSKPSRRLSSRGP 240

Query: 241 KSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKRSRRGGPVRFSQSSSATGADG 300
           KSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKR RR GPVRFSQSSSATGADG
Sbjct: 241 KSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKRPRRCGPVRFSQSSSATGADG 300

Query: 301 ADGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIM 360
           ADGGGIE++YEVNKEKVGAS+G VGSSEKLSWGKGGIRSHTRYGG NGGAGK SRNNRI 
Sbjct: 301 ADGGGIEHEYEVNKEKVGASLGLVGSSEKLSWGKGGIRSHTRYGGTNGGAGKISRNNRIA 360

Query: 361 KLSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETAL 420
           KLSDYVRNSENVSEEELDIH++LVSMD T IPALQRPYICCRPSV IGHLSQYVALET+L
Sbjct: 361 KLSDYVRNSENVSEEELDIHVLLVSMDRT-IPALQRPYICCRPSVMIGHLSQYVALETSL 420

Query: 421 PVDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLV 463
            VDVVE+C+AKELQ+KL+PSTS   KNPCKES+QILNEQETLSTAKLKAHRLACGYL+
Sbjct: 421 SVDVVEICVAKELQVKLDPSTSEATKNPCKESVQILNEQETLSTAKLKAHRLACGYLL 420

[Close]

BLAST of Lsi01G003010 vs. ExPASy TrEMBL
Match: A0A6J1IQZ1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478584 PE=4 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 7.2e-185
Identity = 362/494 (73.28%), Postives = 385/494 (77.94%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVW 60
           MPARKR YETV+ DLLPRLH+TFN+ S+DGESEESDRS SSSQ D+DE            
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSSQEDEDE------------ 60

Query: 61  ASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQC 120
                                                        F+AVKLSDIRR+VQC
Sbjct: 61  ---------------------------------------------FVAVKLSDIRRDVQC 120

Query: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIA 180
           PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIA
Sbjct: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIA 180

Query: 181 VLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKS 240
           VLYPDIDKYEEE              IQ+SIAQTL+RQTEALGRKRSKP RRLSSRG+KS
Sbjct: 181 VLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGQKS 240

Query: 241 FQNHIESLCFDENEDENDYDVSKNSSSADERMDTRP-KRSRRGGPVRFSQSSSATGADGA 300
           FQNHIESL FDENEDENDYD SKNSSSADERMDTRP KRSRRGGPVRFSQSSSAT   GA
Sbjct: 241 FQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSAT---GA 300

Query: 301 DGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMK 360
           DGGG END EVNKEKVGAS+G V SSEK SWGKGGIRSHTRYGGINGG GK SRNNRI K
Sbjct: 301 DGGGNENDCEVNKEKVGASMGLVSSSEKHSWGKGGIRSHTRYGGINGGGGKISRNNRITK 360

Query: 361 LSDYVRNSENVS-EEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETAL 420
           LSDYVRNSEN+S EEELD+HL+LVSMD+TRIPAL+ PYICCRPSVTIGHL Q VAL TAL
Sbjct: 361 LSDYVRNSENISEEEELDVHLMLVSMDKTRIPALRSPYICCRPSVTIGHLRQLVALVTAL 420

Query: 421 PVDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLVCP 479
            VDV+EM + KELQMK+NPSTSG   +PCKES QILN+QETLS  KLK HRLACGYLVC 
Sbjct: 421 SVDVIEMYVVKELQMKINPSTSGAFLDPCKESAQILNQQETLSMDKLKTHRLACGYLVCL 434

[Close]

BLAST of Lsi01G003010 vs. ExPASy TrEMBL
Match: A0A6J1ECT6 (putative E3 ubiquitin-protein ligase RING1a isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433005 PE=4 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 1.5e-182
Identity = 354/477 (74.21%), Postives = 376/477 (78.83%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVW 60
           MPARKR YETV+ DLLPRLH+TFN+ S+DGESEESDRS SSSQ DKDE            
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSSQEDKDE------------ 60

Query: 61  ASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQC 120
                                                        F+AVKLSDIRR+VQC
Sbjct: 61  ---------------------------------------------FVAVKLSDIRRDVQC 120

Query: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIA 180
           PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIA
Sbjct: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIA 180

Query: 181 VLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKS 240
           VLYPDIDKYEEE              IQ+SIAQTL+RQTEALGRKRSKP RRLSSRGRKS
Sbjct: 181 VLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGRKS 240

Query: 241 FQNHIESLCFDENEDENDYDVSKNSSSADERMDTRP-KRSRRGGPVRFSQSSSATGADGA 300
           FQNHIESL FDENEDENDYD SKNSSSADERMDTRP KRSRRGGPVRFSQSSSATGADGA
Sbjct: 241 FQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGADGA 300

Query: 301 DGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMK 360
           DGG  ENDYEVNKE+VGAS+G V SSEKLSWGKGGIRSHTRYGGINGG GK SRNNRI K
Sbjct: 301 DGGSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNNRITK 360

Query: 361 LSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETALP 420
           LSDYVRNSEN+SEEELD+ L+LVSMD+TRIPAL+  YICCRPSVTIGHL Q VALETAL 
Sbjct: 361 LSDYVRNSENISEEELDVQLMLVSMDKTRIPALRSSYICCRPSVTIGHLRQLVALETALS 420

Query: 421 VDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLV 463
           VDV+EM + KELQMK+NPSTSGT  +PCKES QILN+QETLS  KLK HRLACGYL+
Sbjct: 421 VDVIEMYVVKELQMKINPSTSGTFLDPCKESAQILNQQETLSMDKLKTHRLACGYLL 420

[Close]

BLAST of Lsi01G003010 vs. NCBI nr
Match: XP_038881714.1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 [Benincasa hispida])

HSP 1 Score: 699.5 bits (1804), Expect = 2.0e-197
Identity = 376/476 (78.99%), Postives = 392/476 (82.35%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVW 60
           MPARKRAY+TV+VDLLPRLHNTFNECS+DG+SEESDRSPSSSQGDKDE            
Sbjct: 1   MPARKRAYDTVDVDLLPRLHNTFNECSEDGQSEESDRSPSSSQGDKDE------------ 60

Query: 61  ASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQC 120
                                                        FIAVKLSDIR+EVQC
Sbjct: 61  ---------------------------------------------FIAVKLSDIRKEVQC 120

Query: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIA 180
           PICLGII+KTRTVMECLHRFCRECIDKSMRLGN ECPACRTHCASRRSLRDDPNYDTLIA
Sbjct: 121 PICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNYDTLIA 180

Query: 181 VLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKS 240
           VLYPDIDKYEEE              IQTSIAQTLQRQTEALGRKRSKPSRRLSSRG KS
Sbjct: 181 VLYPDIDKYEEEELAFQEEEKGRNKQIQTSIAQTLQRQTEALGRKRSKPSRRLSSRGSKS 240

Query: 241 FQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKRSRRGGPVRFSQSSSATGADGAD 300
           FQNHIES+CFDENEDENDYDVSKNSSSADERMD RPKRSRRGGPVRFSQSSSATGADG D
Sbjct: 241 FQNHIESICFDENEDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATGADGTD 300

Query: 301 GGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKL 360
            GGIENDYEV KEKVGASIG VGSSEKLSWGKGGIRSHTRYGG+NGGAGKN RNNRIMKL
Sbjct: 301 -GGIENDYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNNRIMKL 360

Query: 361 SDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETALPV 420
           SDYVRNSENVSE+ELDIHLVL+S+DETR+PALQRPYI CRPSVTIGHLSQYVAL+T L V
Sbjct: 361 SDYVRNSENVSEKELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALKTGLSV 418

Query: 421 DVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLV 463
           DVVEMC+AKELQMKLNPS SGTLKNPCKE IQILNEQETLSTAK+KAHRLACGYL+
Sbjct: 421 DVVEMCVAKELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYLL 418

[Close]

BLAST of Lsi01G003010 vs. NCBI nr
Match: XP_023543673.1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 679.5 bits (1752), Expect = 2.1e-191
Identity = 367/493 (74.44%), Postives = 389/493 (78.90%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVW 60
           MPARKR YETV+ DLLPRLH+TFN+ S+DGESEESDRS SS Q DKDE            
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSGQEDKDE------------ 60

Query: 61  ASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQC 120
                                                        F+AVKLSDIRR+VQC
Sbjct: 61  ---------------------------------------------FVAVKLSDIRRDVQC 120

Query: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIA 180
           PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIA
Sbjct: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIA 180

Query: 181 VLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKS 240
           VLYPDIDKYEEE              IQ+SIAQTL+RQTEALGRKRSKP RRLSSRGRKS
Sbjct: 181 VLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGRKS 240

Query: 241 FQNHIESLCFDENEDENDYDVSKNSSSADERMDTRP-KRSRRGGPVRFSQSSSATGADGA 300
           FQNHIESL FDENEDENDYD SKNSSSADERMDTRP KRSRRGGPVRFSQSSSATGADGA
Sbjct: 241 FQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGADGA 300

Query: 301 DGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMK 360
           DGG  ENDYEVNKE+VGAS+G V SSEKLSWGKGGIRSHTRYGGINGG GK SRNNRI K
Sbjct: 301 DGGSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNNRITK 360

Query: 361 LSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETALP 420
           LSDYVRNSEN+SEEELD+HL+LVSMD+TRIPAL+ PYICCRPSVTIGHL Q VALETAL 
Sbjct: 361 LSDYVRNSENISEEELDVHLMLVSMDKTRIPALRSPYICCRPSVTIGHLRQLVALETALS 420

Query: 421 VDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLVCPE 479
           VDV+EM + KELQMK+NPSTSGT  +PCKES QILN+QETLS  KLK HRLACGYLVC E
Sbjct: 421 VDVIEMYVVKELQMKINPSTSGTFLDPCKESAQILNQQETLSMDKLKTHRLACGYLVCLE 436

[Close]

BLAST of Lsi01G003010 vs. NCBI nr
Match: XP_022925653.1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 [Cucurbita moschata])

HSP 1 Score: 674.9 bits (1740), Expect = 5.3e-190
Identity = 366/493 (74.24%), Postives = 388/493 (78.70%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVW 60
           MPARKR YETV+ DLLPRLH+TFN+ S+DGESEESDRS SSSQ DKDE            
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSSQEDKDE------------ 60

Query: 61  ASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQC 120
                                                        F+AVKLSDIRR+VQC
Sbjct: 61  ---------------------------------------------FVAVKLSDIRRDVQC 120

Query: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIA 180
           PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIA
Sbjct: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIA 180

Query: 181 VLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKS 240
           VLYPDIDKYEEE              IQ+SIAQTL+RQTEALGRKRSKP RRLSSRGRKS
Sbjct: 181 VLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGRKS 240

Query: 241 FQNHIESLCFDENEDENDYDVSKNSSSADERMDTRP-KRSRRGGPVRFSQSSSATGADGA 300
           FQNHIESL FDENEDENDYD SKNSSSADERMDTRP KRSRRGGPVRFSQSSSATGADGA
Sbjct: 241 FQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGADGA 300

Query: 301 DGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMK 360
           DGG  ENDYEVNKE+VGAS+G V SSEKLSWGKGGIRSHTRYGGINGG GK SRNNRI K
Sbjct: 301 DGGSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNNRITK 360

Query: 361 LSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETALP 420
           LSDYVRNSEN+SEEELD+ L+LVSMD+TRIPAL+  YICCRPSVTIGHL Q VALETAL 
Sbjct: 361 LSDYVRNSENISEEELDVQLMLVSMDKTRIPALRSSYICCRPSVTIGHLRQLVALETALS 420

Query: 421 VDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLVCPE 479
           VDV+EM + KELQMK+NPSTSGT  +PCKES QILN+QETLS  KLK HRLACGYLVC E
Sbjct: 421 VDVIEMYVVKELQMKINPSTSGTFLDPCKESAQILNQQETLSMDKLKTHRLACGYLVCLE 436

[Close]

BLAST of Lsi01G003010 vs. NCBI nr
Match: KAG7034603.1 (putative E3 ubiquitin-protein ligase RING1a, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 671.4 bits (1731), Expect = 5.8e-189
Identity = 379/541 (70.06%), Postives = 404/541 (74.68%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDE------------ 60
           MPARKR YETV+ DLLPRLH+TFN+ S+DGESEESDRS SSSQ DKDE            
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSSQEDKDEYVPFMMSSVFGL 60

Query: 61  ------------------------------------SC------------CDRIRRPAVW 120
                                                C               + RP+  
Sbjct: 61  LLFVRHLLRAVPFYSIFSFDLFHLVGYGVLWCLTAWDCFGICMVCFVVLFSKFLLRPSSK 120

Query: 121 AS----RAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRR 180
            S     +EAFV F PAPE              LVELGAGSC     SRF+AVKLSDIRR
Sbjct: 121 TSCVVFTSEAFVGFYPAPE--------------LVELGAGSC---WLSRFVAVKLSDIRR 180

Query: 181 EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 240
           +VQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+D
Sbjct: 181 DVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFD 240

Query: 241 TLIAVLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSR 300
           TLIAVLYPDIDKYEEE              IQ+SIAQTL+RQTEALGRKRSKP RRLSSR
Sbjct: 241 TLIAVLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSR 300

Query: 301 GRKSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRP-KRSRRGGPVRFSQSSSATG 360
           GRKSFQNHIESL FDENEDENDYD SKNSSSADERMDTRP KRSRRGGP+RFSQSSSATG
Sbjct: 301 GRKSFQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPIRFSQSSSATG 360

Query: 361 ADGADGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNN 420
           ADGADGG  ENDYEVNKE+VGAS+G V SSEKLSWGKGGIRSHTRYGGINGG GK SRNN
Sbjct: 361 ADGADGGSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNN 420

Query: 421 RIMKLSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALE 463
           RI KLSDYVRNSEN+SEEELD+ L+LVSMD+TRIPAL+ PYICCRPSVTIGHL Q VALE
Sbjct: 421 RITKLSDYVRNSENISEEELDVQLMLVSMDKTRIPALRSPYICCRPSVTIGHLRQLVALE 480

[Close]

BLAST of Lsi01G003010 vs. NCBI nr
Match: TYK13150.1 (putative E3 ubiquitin-protein ligase RING1a [Cucumis melo var. makuwa])

HSP 1 Score: 668.3 bits (1723), Expect = 4.9e-188
Identity = 377/511 (73.78%), Postives = 405/511 (79.26%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLL-PRLHNTF-NECSDDGESEESDRSPSSSQGDKDE--SCCDRIRR 60
           MPARKRAY++++VDLL PRLHNTF NE S D +S+ESDRS SSSQGD+DE       +  
Sbjct: 1   MPARKRAYDSLDVDLLPPRLHNTFNNERSQDEQSQESDRSSSSSQGDEDEYVPFMSSVLS 60

Query: 61  PAVWASRAEAFVDFCPAPEFSLLSSFEF--------------LYRLWLVELGAGSCGRSG 120
             ++       V F     F L     +              L+ +  VELGAGSCGRS 
Sbjct: 61  LLLFVRLLFRVVPFYSIYSFDLFRLVGYGVSFVANCLGLIWDLHGVPRVELGAGSCGRSE 120

Query: 121 FSR-----------------FIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECI 180
           FSR                 FIAVKLSDIR+EVQCPICLGII+KTRTVMECLHRFCRECI
Sbjct: 121 FSRLYVRSICFYICYELLGGFIAVKLSDIRKEVQCPICLGIIKKTRTVMECLHRFCRECI 180

Query: 181 DKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE------------- 240
           DKSMRLGN ECPACRTHCASRRSLRDDPNYDTLIAVLYPDI+KYEEE             
Sbjct: 181 DKSMRLGNKECPACRTHCASRRSLRDDPNYDTLIAVLYPDIEKYEEEELAFQEEEKARNK 240

Query: 241 -IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKSFQNHIESLCFDENEDENDYDVSKNS 300
            IQTSIAQTLQRQTE+LGRKRSKPSRRLSSRG KSFQNHIESLCFDENEDENDYDVSKNS
Sbjct: 241 QIQTSIAQTLQRQTESLGRKRSKPSRRLSSRGPKSFQNHIESLCFDENEDENDYDVSKNS 300

Query: 301 SSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGSS 360
           SSADERMDTRPKR RRGGPVRFSQSSSATGADGAD GGIE+DY      VGAS+G VGSS
Sbjct: 301 SSADERMDTRPKRPRRGGPVRFSQSSSATGADGADVGGIEHDY------VGASLGLVGSS 360

Query: 361 EKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSMD 420
           EKLSWGKGGIRSHTRYGG NGGAGKNSRNNRI KLSDYVRNSENVSEEELDIH++LVSMD
Sbjct: 361 EKLSWGKGGIRSHTRYGGTNGGAGKNSRNNRIAKLSDYVRNSENVSEEELDIHVLLVSMD 420

Query: 421 ETRIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAKELQMKLNPSTSGTLKN 463
            + IPALQRPYICCRPSV IGHLSQYVALETAL VDVVE+C+A ELQMKL+PSTS  +KN
Sbjct: 421 RS-IPALQRPYICCRPSVMIGHLSQYVALETALSVDVVEICVANELQMKLDPSTSEAIKN 480

[Close]

BLAST of Lsi01G003010 vs. TAIR 10
Match: AT5G44280.1 (RING 1A )

HSP 1 Score: 283.9 bits (725), Expect = 2.4e-76
Identity = 171/383 (44.65%), Postives = 237/383 (61.88%), Query Frame = 0


Query: 104 SRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHC 163
           S F+ + L +IR++VQCPICLGII+KTRTVMECLHRFCRECIDKSMRLGNNECPACR HC
Sbjct: 120 SEFMEIDLGEIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRKHC 179

Query: 164 ASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQTLQRQTEALG 223
           ASRRSLRDDP +D LIA L+ +ID YEEE              IQ SIAQ  QRQ+EAL 
Sbjct: 180 ASRRSLRDDPKFDALIAALFTNIDSYEEEELAFHEDEMARNKQIQASIAQISQRQSEALV 239

Query: 224 RKRS---------------KPSRRLSSRGRKSFQNHIESLCFDENEDENDYDVSKNSSSA 283
           ++RS                 SRR  +      QN  E+   D+N+D N+    K+SSS 
Sbjct: 240 KRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQQNASEAHEDDDNDDNNNRGRDKDSSSD 299

Query: 284 DERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGSSEKL 343
           +   + R K+ R+    R +Q  S++GA+  +G   +ND EV ++  G S G V + E L
Sbjct: 300 ERGTEVRQKKRRKRSTSRSTQHPSSSGANKNNGNCADNDTEVYRDTKGISPGLVWNPEIL 359

Query: 344 SWGKGGIRSHTRYG-GINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSMDET 403
           +WG+GG RS+TR+G   +GG+ K+ RN R+ KL +Y+R+S + S  ELDIH+ LVS+D  
Sbjct: 360 AWGRGGTRSNTRHGNNTSGGSSKSVRNARVNKLVEYLRSSVDGSSVELDIHVKLVSLDTK 419

Query: 404 RIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAK-----ELQMKLNPSTSGT 452
            IP L +PY+CCRP++ +  L ++VAL+  L  + VE+ + +     +  ++  P+ +  
Sbjct: 420 CIPDLPQPYLCCRPTLLVKQLREFVALQIHLKTEEVELLVTRRRVGEDAAIENLPAVTPA 479

[Close]

BLAST of Lsi01G003010 vs. TAIR 10
Match: AT1G03770.1 (RING 1B )

HSP 1 Score: 245.0 bits (624), Expect = 1.3e-64
Identity = 168/393 (42.75%), Postives = 230/393 (58.52%), Query Frame = 0


Query: 95  AGSCGRSGFSRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 154
           + S G    S ++ V L+DI ++VQC ICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN
Sbjct: 78  SSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 137

Query: 155 ECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQT 214
           ECP CR HCASRRSLRDDPN+D LIA L+ +IDK+EEE              IQ SIAQ 
Sbjct: 138 ECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQIQASIAQV 197

Query: 215 LQRQTEALGRKRS--------KPSRRL--SSRGRKSFQNHIESLCFDENEDENDYDVSKN 274
            QRQ++AL +++S          SRR    SR R++ +N IE    + N+D++     K+
Sbjct: 198 SQRQSKALVKRKSVGKGTAILSRSRRSGGGSRRRRNCRN-IEQDTSEANDDDDQNKRGKD 257

Query: 275 SSSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGS 334
           SSS +     R KRS          SS+A   D   G G E  ++ +   +   +  V +
Sbjct: 258 SSSDEPCERQRKKRS-----ATQPSSSNANNNDNCAGNGTEQTHQRDSRVISPVL--VWN 317

Query: 335 SEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSM 394
           SE ++WG+GG RS+TR G  N GA  + RN R+ +L +Y+ + E  +  ELDIHL LVS+
Sbjct: 318 SELIAWGRGGTRSNTRQGNNNQGA-ISKRNARLKRLVEYLGSLEG-NSVELDIHLKLVSL 377

Query: 395 DETRIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAKELQMKLNPSTSGTLK 454
           D   +  L  PY+C RP++ +  L +YVA    L  + VE+ ++K+    +   TS    
Sbjct: 378 DTEGLLNLHEPYLCFRPTLLVKQLREYVARHLKLKAEEVELLVSKDGDTVIGNKTS---- 437

Query: 455 NPCKESIQILNEQETLSTAKLKAHRLAC-GYLV 463
               E +Q L + ET+  AKLK   ++  GY++
Sbjct: 438 ---TEKMQSLQDDETV--AKLKVDCISSNGYMI 451

[Close]

BLAST of Lsi01G003010 vs. TAIR 10
Match: AT1G03770.3 (RING 1B )

HSP 1 Score: 238.4 bits (607), Expect = 1.2e-62
Identity = 168/399 (42.11%), Postives = 230/399 (57.64%), Query Frame = 0


Query: 95  AGSCGRSGFSRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 154
           + S G    S ++ V L+DI ++VQC ICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN
Sbjct: 78  SSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 137

Query: 155 ECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQT 214
           ECP CR HCASRRSLRDDPN+D LIA L+ +IDK+EEE              IQ SIAQ 
Sbjct: 138 ECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQIQASIAQV 197

Query: 215 LQRQTEALGRKRS--------KPSRRL--SSRGRKSFQNHIESLCFDENEDENDYDVSKN 274
            QRQ++AL +++S          SRR    SR R++ +N IE    + N+D++     K+
Sbjct: 198 SQRQSKALVKRKSVGKGTAILSRSRRSGGGSRRRRNCRN-IEQDTSEANDDDDQNKRGKD 257

Query: 275 SSSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGS 334
           SSS +     R KRS          SS+A   D   G G E  ++ +   +   +  V +
Sbjct: 258 SSSDEPCERQRKKRS-----ATQPSSSNANNNDNCAGNGTEQTHQRDSRVISPVL--VWN 317

Query: 335 SEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSM 394
           SE ++WG+GG RS+TR G  N GA  + RN R+ +L +Y+ + E  +  ELDIHL LVS+
Sbjct: 318 SELIAWGRGGTRSNTRQGNNNQGA-ISKRNARLKRLVEYLGSLEG-NSVELDIHLKLVSL 377

Query: 395 DETRIPALQRPYICCRPSVTIGHLSQ------YVALETALPVDVVEMCMAKELQMKLNPS 454
           D   +  L  PY+C RP++ +  L +      YVA    L  + VE+ ++K+    +   
Sbjct: 378 DTEGLLNLHEPYLCFRPTLLVKQLREVSSLPLYVARHLKLKAEEVELLVSKDGDTVIGNK 437

Query: 455 TSGTLKNPCKESIQILNEQETLSTAKLKAHRLAC-GYLV 463
           TS        E +Q L + ET+  AKLK   ++  GY++
Sbjct: 438 TS-------TEKMQSLQDDETV--AKLKVDCISSNGYMI 457

[Close]

BLAST of Lsi01G003010 vs. TAIR 10
Match: AT1G03770.2 (RING 1B )

HSP 1 Score: 238.4 bits (607), Expect = 1.2e-62
Identity = 168/399 (42.11%), Postives = 230/399 (57.64%), Query Frame = 0


Query: 95  AGSCGRSGFSRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 154
           + S G    S ++ V L+DI ++VQC ICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN
Sbjct: 80  SSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 139

Query: 155 ECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQT 214
           ECP CR HCASRRSLRDDPN+D LIA L+ +IDK+EEE              IQ SIAQ 
Sbjct: 140 ECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQIQASIAQV 199

Query: 215 LQRQTEALGRKRS--------KPSRRL--SSRGRKSFQNHIESLCFDENEDENDYDVSKN 274
            QRQ++AL +++S          SRR    SR R++ +N IE    + N+D++     K+
Sbjct: 200 SQRQSKALVKRKSVGKGTAILSRSRRSGGGSRRRRNCRN-IEQDTSEANDDDDQNKRGKD 259

Query: 275 SSSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGS 334
           SSS +     R KRS          SS+A   D   G G E  ++ +   +   +  V +
Sbjct: 260 SSSDEPCERQRKKRS-----ATQPSSSNANNNDNCAGNGTEQTHQRDSRVISPVL--VWN 319

Query: 335 SEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSM 394
           SE ++WG+GG RS+TR G  N GA  + RN R+ +L +Y+ + E  +  ELDIHL LVS+
Sbjct: 320 SELIAWGRGGTRSNTRQGNNNQGA-ISKRNARLKRLVEYLGSLEG-NSVELDIHLKLVSL 379

Query: 395 DETRIPALQRPYICCRPSVTIGHLSQ------YVALETALPVDVVEMCMAKELQMKLNPS 454
           D   +  L  PY+C RP++ +  L +      YVA    L  + VE+ ++K+    +   
Sbjct: 380 DTEGLLNLHEPYLCFRPTLLVKQLREVSSLPLYVARHLKLKAEEVELLVSKDGDTVIGNK 439

Query: 455 TSGTLKNPCKESIQILNEQETLSTAKLKAHRLAC-GYLV 463
           TS        E +Q L + ET+  AKLK   ++  GY++
Sbjct: 440 TS-------TEKMQSLQDDETV--AKLKVDCISSNGYMI 459

[Close]

BLAST of Lsi01G003010 vs. TAIR 10
Match: AT5G44280.2 (RING 1A )

HSP 1 Score: 217.2 bits (552), Expect = 2.8e-56
Identity = 147/387 (37.98%), Postives = 220/387 (56.85%), Query Frame = 0


Query: 104 SRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMR----LGNNECPAC 163
           S F+ + L +IR++VQCPICL  ++  R  +   ++  ++C   S +      NNECPAC
Sbjct: 120 SEFMEIDLGEIRKDVQCPICLVGMKFLRLFIS-YYKENKDCDGMSPQFCATFKNNECPAC 179

Query: 164 RTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQTLQRQT 223
           R HCASRRSLRDDP +D LIA L+ +ID YEEE              IQ SIAQ  QRQ+
Sbjct: 180 RKHCASRRSLRDDPKFDALIAALFTNIDSYEEEELAFHEDEMARNKQIQASIAQISQRQS 239

Query: 224 EALGRKRS---------------KPSRRLSSRGRKSFQNHIESLCFDENEDENDYDVSKN 283
           EAL ++RS                 SRR  +      QN  E+   D+N+D N+    K+
Sbjct: 240 EALVKRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQQNASEAHEDDDNDDNNNRGRDKD 299

Query: 284 SSSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGS 343
           SSS +   + R K+ R+    R +Q  S++GA+  +G   +ND EV ++  G S G V +
Sbjct: 300 SSSDERGTEVRQKKRRKRSTSRSTQHPSSSGANKNNGNCADNDTEVYRDTKGISPGLVWN 359

Query: 344 SEKLSWGKGGIRSHTRYG-GINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVS 403
            E L+WG+GG RS+TR+G   +GG+ K+ RN R+ KL +Y+R+S + S  ELDIH+ LVS
Sbjct: 360 PEILAWGRGGTRSNTRHGNNTSGGSSKSVRNARVNKLVEYLRSSVDGSSVELDIHVKLVS 419

Query: 404 MDETRIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAK-----ELQMKLNPS 452
           +D   IP L +PY+CCRP++ +  L ++VAL+  L  + VE+ + +     +  ++  P+
Sbjct: 420 LDTKCIPDLPQPYLCCRPTLLVKQLREFVALQIHLKTEEVELLVTRRRVGEDAAIENLPA 479

The following BLAST results are available for this feature:

BLAST of Lsi01G003010 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FKW0	3.4e-75	44.65	Putative E3 ubiquitin-protein ligase RING1a OS=Arabidopsis thaliana OX=3702 GN=R...	[more]
Q0WX00	1.8e-63	42.75	Putative E3 ubiquitin-protein ligase RING1b OS=Arabidopsis thaliana OX=3702 GN=R...	[more]
Q5TJF3	9.2e-20	33.50	E3 ubiquitin-protein ligase RING1 OS=Canis lupus familiaris OX=9615 GN=RING1 PE=...	[more]
A1YER5	9.2e-20	33.50	E3 ubiquitin-protein ligase RING1 OS=Gorilla gorilla gorilla OX=9595 GN=RING1 PE...	[more]
Q06587	2.7e-19	32.99	E3 ubiquitin-protein ligase RING1 OS=Homo sapiens OX=9606 GN=RING1 PE=1 SV=2	[more]

BLAST of Lsi01G003010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EFU8	2.5e-190	74.24	putative E3 ubiquitin-protein ligase RING1a isoform X1 OS=Cucurbita moschata OX=...	[more]
A0A5D3CNZ3	2.4e-188	73.78	Putative E3 ubiquitin-protein ligase RING1a OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A0A0KLI2	8.5e-186	75.94	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517990 P...	[more]
A0A6J1IQZ1	7.2e-185	73.28	putative E3 ubiquitin-protein ligase RING1a isoform X1 OS=Cucurbita maxima OX=36...	[more]
A0A6J1ECT6	1.5e-182	74.21	putative E3 ubiquitin-protein ligase RING1a isoform X2 OS=Cucurbita moschata OX=...	[more]

BLAST of Lsi01G003010 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881714.1	2.0e-197	78.99	putative E3 ubiquitin-protein ligase RING1a isoform X1 [Benincasa hispida]	[more]
XP_023543673.1	2.1e-191	74.44	putative E3 ubiquitin-protein ligase RING1a isoform X1 [Cucurbita pepo subsp. pe...	[more]
XP_022925653.1	5.3e-190	74.24	putative E3 ubiquitin-protein ligase RING1a isoform X1 [Cucurbita moschata]	[more]
KAG7034603.1	5.8e-189	70.06	putative E3 ubiquitin-protein ligase RING1a, partial [Cucurbita argyrosperma sub...	[more]
TYK13150.1	4.9e-188	73.78	putative E3 ubiquitin-protein ligase RING1a [Cucumis melo var. makuwa]	[more]

BLAST of Lsi01G003010 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G44280.1	2.4e-76	44.65	RING 1A	[more]
AT1G03770.1	1.3e-64	42.75	RING 1B	[more]
AT1G03770.3	1.2e-62	42.11	RING 1B	[more]
AT1G03770.2	1.2e-62	42.11	RING 1B	[more]
AT5G44280.2	2.8e-56	37.98	RING 1A	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 120..159 e-value: 9.7E-7 score: 38.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 120..160 score: 12.076194
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 104..208 e-value: 2.9E-18 score: 67.7
None	No IPR available	PFAM	PF13923	zf-C3HC4_2	coord: 119..159 e-value: 9.8E-12 score: 44.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 204..226
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 24..49
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 245..266
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 241..292
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 33..49
None	No IPR available	PANTHER	PTHR46537:SF3	OS11G0578200 PROTEIN	coord: 1..452
None	No IPR available	CDD	cd16531	RING-HC_RING1_like	coord: 120..160 e-value: 4.21225E-21 score: 84.0207
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 111..192
IPR044592	Putative E3 ubiquitin-protein ligase RING1a/b	PANTHER	PTHR46537	OS11G0578200 PROTEIN	coord: 1..452
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 136..145

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G003010.1	Lsi01G003010.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding