Lsi01G003010.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi01G003010.1
TypemRNA
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionRING-type domain-containing protein
Locationchr01: 2689575 .. 2694329 (-)
Sequence length1635
RNA-Seq ExpressionLsi01G003010.1
SyntenyLsi01G003010.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTGGTGTGTTTTTCTTTAATCAATCTAATGCCGTGGAAATTTCGTAAAACCACGAACACGAGGCTCCTTCCCTCTCGGCTCTGATATTTTGCTCCACCGCCCCCTTCATCATCTCTACGAACCTCAATTTTTGTGAACTTACAACTTCTTTTCGTTGTTTCTTCAACTTATTTTGCTTCAGACCGAAGCAATGCCGGCTCGGAAGCGCGCTTACGAGACTGTGAATGTTGATCTTTTGCCGCGACTCCATAATACCTTCAATGAATGCTCTGACGATGGAGAATCTGAGGGTACGCTTCTTGTTTCTCTTCAGCACCCTAAATTTTCACGATGCTACCGACTCTTTGGTGTTCCTGCATTTCATATATGTTATTATTTGTTTGTTTAATTCTTTTTTTTGTGCGTCTACTGAGTTCGTGGTGTTCTATGTTGCTCCTTCTTTCGCCAGAATCCGATCGAAGCCCTTCGTCCAGTCAAGGAGATAAGGACGAGTATGTTCCGTTTATGAACTCTGTTTTTCGCTTGCTTCTATTTGTTCGTCATCTTCTTCGTGCTCTTCCTTTTTACTCTATCTACTCTTTTGATTTATTTCCTCTGGTTGGTTATGGAGTTCTTTGGTGCTTAACTGCTTGGGATTGATTTGGGATCTGCATGGTATGCTTCGTGGTATTGTTCATCGAGGTCTTGCTGCGACCGTATTCGAAGACCTGCTGTGTGGGCTTCACGTGCTGAAGCATTCGTCGATTTTTGTCCTGCACCGGAGTTTTCTTTACTTTCAAGTTTTGAGTTTCTTTACCGATTATGGTTAGTGGAATTGGGTGCTGGATCTTGTGGGCGAAGTGGGTTCTCGAGGTCGTATATCCGATCAATCTGCTTTTACATATGCTACGAGCTGCTGGGTGGGTAATGACGTTGGTGCTCACATGGTTTTAGCTTCTTTACGATATTAAAGCTTACATGATCCGAGATCAAGCTTTCACTCAACGTGAAATCAAGCTTATTATAACTGAAGCGTTCACGGAAAAATCTTGCTGGTTCCCGCCATTGATTGTCGGATCCAATTTTACGCAAATTCCAAGAAGTTAACGACTGGAAACCCGACATTTCATTAAGAACGATTTTACCCAAATTTTTTACAACTTGAGCGGTACTCTTACGCTAGCGCGATGAACGAACTATTTGGGTTGCGTTATAGAATATATGTATCTGAACAGAAAGTTGTTTATCGGTTCTTTAGTTGCATGGTGAACAGTAGAATTCCCTGAACGATTAACTCGATCTTAGGTGTCTTGCGATTATTCCGACATCCTAAATCTTCTGTCTGGTTTTCTTTTCCAGATTTATCGCCGTGAAATTGTCAGACATTCGGAGAGAAGTTCAATGTCCTATCTGCTTAGGTATGTTTTAATAATCTCTTCTTGTTGAACGTATTTATCTCATATAAGATAAGTTTTCTATTGATTTGAACATTTGGTTTTAATTCTTATACAATTCATGTTCTGCAAGTATATAAACACGCCTACTACTTGAAACGACTTTGTGTTTACCATTGGTGTCAATGTGTCATCCAGGTCTTTACTGACTTTAACAAGTAAGATTGTTCTCATATCCTGAGAATCAATCATCGTGTATAATTAACTATTTTCGTAAGTTTATGTACATACGCACTGCAAGATGACCTATCAATAAGGTTATTTCAAATGATTGATTTGTCCGGGATGAATTGTGGAGGATGAGAGCATTAATTTTAAATGAAGTGTTGAATCAAGTGCCACCGGACTCATCTGATCCAAGAATTTATTCTGATATAACAAATTTGATTTTCTGTATATAAGTTGTATCAAATAACATAAAGATTGTTTGTGATCTCCGGATCTGGGAGTGATGGCAATCATTAAAATCTGAGGTTGACTTTGTATATGGACTATCGATTTAGATATGGATTCTGATATGGCTTCTTTATCAGTTTTTGCTTTGGCTTAAAAAAGTTATTAGTCAGTTGTCACCCCCCCTCCACTTCCAGGGTCTTGCATATAAAACAATTGCATTTTTACGTTCCATGGACCTCTGTTTGTATTTTAGGTGACACTTATAATTGCAATCCTTCTGCTTTTCTTATTCCCCACAAGTAACTTAGAAGTTAAGATGACAAACTTTTCTACCTCCACTTATTTGTCCTAACATCAACTTCGTTATCGAGTTTTGTATTTTTTGATTAGACCATTCCGTTAAAGTAACTAATTGGACCGTAGAGTGTGAATTCATGAGCATATGAGGGTATGGAATTTGGTGATCTGCATTCTTTTTCGAACCTTACCGTTTCAGCCTTTCAGGCTACATAAGTTTATGTGGAGCCAGTAATTTATGATGGTGTTACATGTTTACACATAACAAGTAGTAAATATATGCATGACTTTCAAATTATTGATTACTCAAGCTCTAATTGTCAAGATTATTGATCCTTACTTTCAGTTCATGCAGTATCTGATAAAGGAAACACCCAAAAATCTTCCTTATCATAAGACAATAACTAAATTCCAACTCGAAACATTCCTGAAATTTTACATTTTTTAGTGGATGTATCCAAATACTATGAGCCATGACATGCTAAGTCTTAGCATGTTTGGGAAGTATATTTAGGTGGATTTGGTGAGGCAACCTCAGAGTTCACTTTGGAAGGTCATCTCCATGTTTATGCATGAGTAGTATTCTTAAGGAGGTTGATAAAGTTTTTTCAATTCCTAATGTGCAGGAATCATCAGAAAAACCAGAACAGTGATGGAATGTCTGCACCGCTTCTGCAGGGAATGCATAGACAAATCGATGCGTCTAGGGTATGTTCTTATGTAATTTTTTATTTTGATCTTGCTAATTGCTATACAGCTCAAGAAAATAAATATATATCGCAGCACAACTTTCCTTCTGGCTTCCACTAAACTTTTACTGAAATTTGCTGCAGTAACAATGAGTGCCCTGCTTGCCGCACTCATTGTGCCAGTCGACGCTCTCTGAGAGATGATCCGAATTATGATACATTGATTGCTGTGTTATATCCAGATATTGACAAATATGAGGAAGAGGTGGGAATATTATGTTTAAGCTTGCAAGCTTGGTGTTCGATGATTGATATCGTCATTTCTGCACACTCCACTATTGTTAATTTTTTTTTTTTTTTTTTTTTTGGGAACTGGCTTTCCAAGAAGAAGAGAAAGCTCGTAATAAGCAGGTGAGAAGGCAAAGTTCCGTGCATTTTTTTCGTTATTGACATGAATTTGTTTATTTTCCAGAGTTGATGAGCTCACTTTACATTGTTCGATTACAAGAAACCACAGATTTTGTCATGCTAAAATTGATTGAGAGAGAGAGAGAGAGGAATTATTTAAATCATTATTTGATAAATATGTAATGCAAAATTGTGGGAGCATTGGGACATCAAATAGAGCATGTTTTTCTCAGCTGTTGTGCTTTTCCATTCCATTTTACTTGGGAACATCTCGAGCTGAAAACCAACATGGCTATTGTAGATTGTACATGTATCAGGTATCTATGATGACACTTGATCCTTATTCTTCATACTTGGTCTAAAATGTTACTTTACGGTTATTTTGCAGATTCAAACTTCCATTGCTCAGACACTTCAACGACAAACTGAAGCTTTGGGTAGAAAACGTTCTAAACCTTCGAGGAGATTAAGTTCGAGAGGACGCAAAAGCTTCCAAAATCATATTGAGAGTCTATGCTTCGATGAAAATGAGGACGAAAATGATTATGATGTCAGCAAAAATTCATCTTCTGCTGATGAGCGCATGGACACCAGACCTAAAAGATCCAGGAGAGGTGGTCCTGTTAGATTTTCCCAGTCTTCTTCAGCTACTGGAGCAGATGGAGCAGATGGTGGTGGTATCGAAAATGATTATGAAGTGAACAAAGAAAAAGTTGGTGCATCTATAGGGTTTGTTGGTAGCTCAGAAAAACTTTCCTGGGGCAAAGGTGGAATAAGAAGTCACACTAGATATGGGGGTATAAATGGGGGGGCTGGTAAAAATTCCAGGAATAACCGCATCATGAAGCTGTCGGATTATGTTCGAAACTCAGAAAATGTCAGTGAGGAGGAGGTAAAAACAACAATTTGAGGTGTTACTAAACTTCCTAATGAATAAACTGTGGACATGTTAACGTATAAATGGCTCGCCTATTGAATCACATGCATGCTGTTATGCCTCTTTTAGCTGCTTAAAAGCTCAACGATCTTTTATGTTTCTATCCATCAATGACTTTTAACTTCTCTTCCTGACTTTTAGTTGGACATCCACCTTGTGCTCGTTTCCATGGATGAAACTAGAATACCCGCATTGCAAAGACCATATATTTGCTGCAGGCCTTCAGTGACGATTGGACACCTAAGTCAAGTAATCTGTCATTAAATGGAGCTGCACTTCATCCGTTTAAGGTCTTATTCATTTATTTAGTTGATTCTTGAGCTTCCTTTCTTCTGCAGTATGTCGCTCTCGAAACCGCGCTTCCCGTTGATGTTGTTGAAATGTGCATGGCGAAAGAGCTGCAAATGAAACTCAATCCTTCAACCTCCGGAACTCTAAAGAACCCATGTAAAGAAAGTATACAGATACTAAACGAGCAAGAAACGCTATCGACAGCCAAACTTAAAGCACACAGGCTAGCTTGTGGCTATCTGGTATGCCCTGAATCTACAGCACACAAATGCTTGAAAAAAATGCACCCACTACATCAATAA

mRNA sequence

CTTTGGTGTGTTTTTCTTTAATCAATCTAATGCCGTGGAAATTTCGTAAAACCACGAACACGAGGCTCCTTCCCTCTCGGCTCTGATATTTTGCTCCACCGCCCCCTTCATCATCTCTACGAACCTCAATTTTTGTGAACTTACAACTTCTTTTCGTTGTTTCTTCAACTTATTTTGCTTCAGACCGAAGCAATGCCGGCTCGGAAGCGCGCTTACGAGACTGTGAATGTTGATCTTTTGCCGCGACTCCATAATACCTTCAATGAATGCTCTGACGATGGAGAATCTGAGGAATCCGATCGAAGCCCTTCGTCCAGTCAAGGAGATAAGGACGAGTCTTGCTGCGACCGTATTCGAAGACCTGCTGTGTGGGCTTCACGTGCTGAAGCATTCGTCGATTTTTGTCCTGCACCGGAGTTTTCTTTACTTTCAAGTTTTGAGTTTCTTTACCGATTATGGTTAGTGGAATTGGGTGCTGGATCTTGTGGGCGAAGTGGGTTCTCGAGATTTATCGCCGTGAAATTGTCAGACATTCGGAGAGAAGTTCAATGTCCTATCTGCTTAGGAATCATCAGAAAAACCAGAACAGTGATGGAATGTCTGCACCGCTTCTGCAGGGAATGCATAGACAAATCGATGCGTCTAGGTAACAATGAGTGCCCTGCTTGCCGCACTCATTGTGCCAGTCGACGCTCTCTGAGAGATGATCCGAATTATGATACATTGATTGCTGTGTTATATCCAGATATTGACAAATATGAGGAAGAGATTCAAACTTCCATTGCTCAGACACTTCAACGACAAACTGAAGCTTTGGGTAGAAAACGTTCTAAACCTTCGAGGAGATTAAGTTCGAGAGGACGCAAAAGCTTCCAAAATCATATTGAGAGTCTATGCTTCGATGAAAATGAGGACGAAAATGATTATGATGTCAGCAAAAATTCATCTTCTGCTGATGAGCGCATGGACACCAGACCTAAAAGATCCAGGAGAGGTGGTCCTGTTAGATTTTCCCAGTCTTCTTCAGCTACTGGAGCAGATGGAGCAGATGGTGGTGGTATCGAAAATGATTATGAAGTGAACAAAGAAAAAGTTGGTGCATCTATAGGGTTTGTTGGTAGCTCAGAAAAACTTTCCTGGGGCAAAGGTGGAATAAGAAGTCACACTAGATATGGGGGTATAAATGGGGGGGCTGGTAAAAATTCCAGGAATAACCGCATCATGAAGCTGTCGGATTATGTTCGAAACTCAGAAAATGTCAGTGAGGAGGAGTTGGACATCCACCTTGTGCTCGTTTCCATGGATGAAACTAGAATACCCGCATTGCAAAGACCATATATTTGCTGCAGGCCTTCAGTGACGATTGGACACCTAAGTCAATATGTCGCTCTCGAAACCGCGCTTCCCGTTGATGTTGTTGAAATGTGCATGGCGAAAGAGCTGCAAATGAAACTCAATCCTTCAACCTCCGGAACTCTAAAGAACCCATGTAAAGAAAGTATACAGATACTAAACGAGCAAGAAACGCTATCGACAGCCAAACTTAAAGCACACAGGCTAGCTTGTGGCTATCTGGTATGCCCTGAATCTACAGCACACAAATGCTTGAAAAAAATGCACCCACTACATCAATAA

Coding sequence (CDS)

ATGCCGGCTCGGAAGCGCGCTTACGAGACTGTGAATGTTGATCTTTTGCCGCGACTCCATAATACCTTCAATGAATGCTCTGACGATGGAGAATCTGAGGAATCCGATCGAAGCCCTTCGTCCAGTCAAGGAGATAAGGACGAGTCTTGCTGCGACCGTATTCGAAGACCTGCTGTGTGGGCTTCACGTGCTGAAGCATTCGTCGATTTTTGTCCTGCACCGGAGTTTTCTTTACTTTCAAGTTTTGAGTTTCTTTACCGATTATGGTTAGTGGAATTGGGTGCTGGATCTTGTGGGCGAAGTGGGTTCTCGAGATTTATCGCCGTGAAATTGTCAGACATTCGGAGAGAAGTTCAATGTCCTATCTGCTTAGGAATCATCAGAAAAACCAGAACAGTGATGGAATGTCTGCACCGCTTCTGCAGGGAATGCATAGACAAATCGATGCGTCTAGGTAACAATGAGTGCCCTGCTTGCCGCACTCATTGTGCCAGTCGACGCTCTCTGAGAGATGATCCGAATTATGATACATTGATTGCTGTGTTATATCCAGATATTGACAAATATGAGGAAGAGATTCAAACTTCCATTGCTCAGACACTTCAACGACAAACTGAAGCTTTGGGTAGAAAACGTTCTAAACCTTCGAGGAGATTAAGTTCGAGAGGACGCAAAAGCTTCCAAAATCATATTGAGAGTCTATGCTTCGATGAAAATGAGGACGAAAATGATTATGATGTCAGCAAAAATTCATCTTCTGCTGATGAGCGCATGGACACCAGACCTAAAAGATCCAGGAGAGGTGGTCCTGTTAGATTTTCCCAGTCTTCTTCAGCTACTGGAGCAGATGGAGCAGATGGTGGTGGTATCGAAAATGATTATGAAGTGAACAAAGAAAAAGTTGGTGCATCTATAGGGTTTGTTGGTAGCTCAGAAAAACTTTCCTGGGGCAAAGGTGGAATAAGAAGTCACACTAGATATGGGGGTATAAATGGGGGGGCTGGTAAAAATTCCAGGAATAACCGCATCATGAAGCTGTCGGATTATGTTCGAAACTCAGAAAATGTCAGTGAGGAGGAGTTGGACATCCACCTTGTGCTCGTTTCCATGGATGAAACTAGAATACCCGCATTGCAAAGACCATATATTTGCTGCAGGCCTTCAGTGACGATTGGACACCTAAGTCAATATGTCGCTCTCGAAACCGCGCTTCCCGTTGATGTTGTTGAAATGTGCATGGCGAAAGAGCTGCAAATGAAACTCAATCCTTCAACCTCCGGAACTCTAAAGAACCCATGTAAAGAAAGTATACAGATACTAAACGAGCAAGAAACGCTATCGACAGCCAAACTTAAAGCACACAGGCTAGCTTGTGGCTATCTGGTATGCCCTGAATCTACAGCACACAAATGCTTGAAAAAAATGCACCCACTACATCAATAA

Protein sequence

MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVWASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEEIQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLVCPESTAHKCLKKMHPLHQ
Homology
BLAST of Lsi01G003010.1 vs. ExPASy Swiss-Prot
Match: Q9FKW0 (Putative E3 ubiquitin-protein ligase RING1a OS=Arabidopsis thaliana OX=3702 GN=RING1A PE=1 SV=2)

HSP 1 Score: 283.9 bits (725), Expect = 3.4e-75
Identity = 171/383 (44.65%), Postives = 237/383 (61.88%), Query Frame = 0

Query: 104 SRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHC 163
           S F+ + L +IR++VQCPICLGII+KTRTVMECLHRFCRECIDKSMRLGNNECPACR HC
Sbjct: 120 SEFMEIDLGEIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRKHC 179

Query: 164 ASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQTLQRQTEALG 223
           ASRRSLRDDP +D LIA L+ +ID YEEE              IQ SIAQ  QRQ+EAL 
Sbjct: 180 ASRRSLRDDPKFDALIAALFTNIDSYEEEELAFHEDEMARNKQIQASIAQISQRQSEALV 239

Query: 224 RKRS---------------KPSRRLSSRGRKSFQNHIESLCFDENEDENDYDVSKNSSSA 283
           ++RS                 SRR  +      QN  E+   D+N+D N+    K+SSS 
Sbjct: 240 KRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQQNASEAHEDDDNDDNNNRGRDKDSSSD 299

Query: 284 DERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGSSEKL 343
           +   + R K+ R+    R +Q  S++GA+  +G   +ND EV ++  G S G V + E L
Sbjct: 300 ERGTEVRQKKRRKRSTSRSTQHPSSSGANKNNGNCADNDTEVYRDTKGISPGLVWNPEIL 359

Query: 344 SWGKGGIRSHTRYG-GINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSMDET 403
           +WG+GG RS+TR+G   +GG+ K+ RN R+ KL +Y+R+S + S  ELDIH+ LVS+D  
Sbjct: 360 AWGRGGTRSNTRHGNNTSGGSSKSVRNARVNKLVEYLRSSVDGSSVELDIHVKLVSLDTK 419

Query: 404 RIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAK-----ELQMKLNPSTSGT 452
            IP L +PY+CCRP++ +  L ++VAL+  L  + VE+ + +     +  ++  P+ +  
Sbjct: 420 CIPDLPQPYLCCRPTLLVKQLREFVALQIHLKTEEVELLVTRRRVGEDAAIENLPAVTPA 479

BLAST of Lsi01G003010.1 vs. ExPASy Swiss-Prot
Match: Q0WX00 (Putative E3 ubiquitin-protein ligase RING1b OS=Arabidopsis thaliana OX=3702 GN=RING1B PE=1 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.8e-63
Identity = 168/393 (42.75%), Postives = 230/393 (58.52%), Query Frame = 0

Query: 95  AGSCGRSGFSRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 154
           + S G    S ++ V L+DI ++VQC ICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN
Sbjct: 78  SSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 137

Query: 155 ECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQT 214
           ECP CR HCASRRSLRDDPN+D LIA L+ +IDK+EEE              IQ SIAQ 
Sbjct: 138 ECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQIQASIAQV 197

Query: 215 LQRQTEALGRKRS--------KPSRRL--SSRGRKSFQNHIESLCFDENEDENDYDVSKN 274
            QRQ++AL +++S          SRR    SR R++ +N IE    + N+D++     K+
Sbjct: 198 SQRQSKALVKRKSVGKGTAILSRSRRSGGGSRRRRNCRN-IEQDTSEANDDDDQNKRGKD 257

Query: 275 SSSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGS 334
           SSS +     R KRS          SS+A   D   G G E  ++ +   +   +  V +
Sbjct: 258 SSSDEPCERQRKKRS-----ATQPSSSNANNNDNCAGNGTEQTHQRDSRVISPVL--VWN 317

Query: 335 SEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSM 394
           SE ++WG+GG RS+TR G  N GA  + RN R+ +L +Y+ + E  +  ELDIHL LVS+
Sbjct: 318 SELIAWGRGGTRSNTRQGNNNQGA-ISKRNARLKRLVEYLGSLEG-NSVELDIHLKLVSL 377

Query: 395 DETRIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAKELQMKLNPSTSGTLK 454
           D   +  L  PY+C RP++ +  L +YVA    L  + VE+ ++K+    +   TS    
Sbjct: 378 DTEGLLNLHEPYLCFRPTLLVKQLREYVARHLKLKAEEVELLVSKDGDTVIGNKTS---- 437

Query: 455 NPCKESIQILNEQETLSTAKLKAHRLAC-GYLV 463
               E +Q L + ET+  AKLK   ++  GY++
Sbjct: 438 ---TEKMQSLQDDETV--AKLKVDCISSNGYMI 451

BLAST of Lsi01G003010.1 vs. ExPASy Swiss-Prot
Match: Q5TJF3 (E3 ubiquitin-protein ligase RING1 OS=Canis lupus familiaris OX=9615 GN=RING1 PE=3 SV=2)

HSP 1 Score: 99.8 bits (247), Expect = 9.2e-20
Identity = 66/197 (33.50%), Postives = 94/197 (47.72%), Query Frame = 0

Query: 107 IAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASR 166
           IAV    +  E+ CPICL +++ T T  ECLHRFC +CI  ++R GN ECP CR    S+
Sbjct: 35  IAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSK 94

Query: 167 RSLRDDPNYDTLIAVLYPDIDKYEEE-----IQTSIAQTLQRQTEALGRKRSKPSRRLSS 226
           RSLR DPN+D LI+ +YP  ++YE       I+ S     Q  + ++       +   + 
Sbjct: 95  RSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQ 154

Query: 227 RGRKSFQNHIESLCF-----DENEDENDYDVSKNSSSADERMDTRPKRSRRGGPVRFSQS 286
           R R+      ++        +  E E D +   + S+ D      PKR R GG    S  
Sbjct: 155 RVRRPMPGSDQTTTMSGGEGEPGEGEGDGEDVSSDSAPDSAPGPAPKRPRGGGAGGSSVG 214

Query: 287 SSATGADGADGGGIEND 294
           +   GA G  GG    D
Sbjct: 215 TGGGGAGGVGGGAGSED 231

BLAST of Lsi01G003010.1 vs. ExPASy Swiss-Prot
Match: A1YER5 (E3 ubiquitin-protein ligase RING1 OS=Gorilla gorilla gorilla OX=9595 GN=RING1 PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 9.2e-20
Identity = 66/197 (33.50%), Postives = 93/197 (47.21%), Query Frame = 0

Query: 107 IAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASR 166
           IAV    +  E+ CPICL +++ T T  ECLHRFC +CI  ++R GN ECP CR    S+
Sbjct: 6   IAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSK 65

Query: 167 RSLRDDPNYDTLIAVLYPDIDKYEEE-----IQTSIAQTLQRQTEALGRKRSKPSRRLSS 226
           RSLR DPN+D LI+ +YP  ++YE       I+ S     Q  + ++       +   + 
Sbjct: 66  RSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQ 125

Query: 227 RGRKSFQNHIESLCF-----DENEDENDYDVSKNSSSADERMDTRPKRSRRGGPVRFSQS 286
           R R+      ++        D  E E D +   + S+ D      PKR R GG    S  
Sbjct: 126 RVRRPIPGSDQTTTMSGGEGDPGEGEGDGEDVSSDSAPDSAPGPAPKRPRGGGAGGSSVG 185

Query: 287 SSATGADGADGGGIEND 294
           +   G  G  GG    D
Sbjct: 186 TGGGGTGGVGGGAGSED 202

BLAST of Lsi01G003010.1 vs. ExPASy Swiss-Prot
Match: Q06587 (E3 ubiquitin-protein ligase RING1 OS=Homo sapiens OX=9606 GN=RING1 PE=1 SV=2)

HSP 1 Score: 98.2 bits (243), Expect = 2.7e-19
Identity = 65/197 (32.99%), Postives = 93/197 (47.21%), Query Frame = 0

Query: 107 IAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASR 166
           IAV    +  E+ CPICL +++ T T  ECLHRFC +CI  ++R GN ECP CR    S+
Sbjct: 35  IAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSK 94

Query: 167 RSLRDDPNYDTLIAVLYPDIDKYEEE-----IQTSIAQTLQRQTEALGRKRSKPSRRLSS 226
           RSLR DPN+D LI+ +YP  ++YE       I+ S     Q  + ++       +   + 
Sbjct: 95  RSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQ 154

Query: 227 RGRKSFQNHIESLCF-----DENEDENDYDVSKNSSSADERMDTRPKRSRRGGPVRFSQS 286
           R R+      ++        +  E E D +   + S+ D      PKR R GG    S  
Sbjct: 155 RVRRPIPGSDQTTTMSGGEGEPGEGEGDGEDVSSDSAPDSAPGPAPKRPRGGGAGGSSVG 214

Query: 287 SSATGADGADGGGIEND 294
           +   G  G  GG    D
Sbjct: 215 TGGGGTGGVGGGAGSED 231

BLAST of Lsi01G003010.1 vs. ExPASy TrEMBL
Match: A0A6J1EFU8 (putative E3 ubiquitin-protein ligase RING1a isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433005 PE=4 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 2.5e-190
Identity = 366/493 (74.24%), Postives = 388/493 (78.70%), Query Frame = 0

Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVW 60
           MPARKR YETV+ DLLPRLH+TFN+ S+DGESEESDRS SSSQ DKDE            
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSSQEDKDE------------ 60

Query: 61  ASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQC 120
                                                        F+AVKLSDIRR+VQC
Sbjct: 61  ---------------------------------------------FVAVKLSDIRRDVQC 120

Query: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIA 180
           PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIA
Sbjct: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIA 180

Query: 181 VLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKS 240
           VLYPDIDKYEEE              IQ+SIAQTL+RQTEALGRKRSKP RRLSSRGRKS
Sbjct: 181 VLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGRKS 240

Query: 241 FQNHIESLCFDENEDENDYDVSKNSSSADERMDTRP-KRSRRGGPVRFSQSSSATGADGA 300
           FQNHIESL FDENEDENDYD SKNSSSADERMDTRP KRSRRGGPVRFSQSSSATGADGA
Sbjct: 241 FQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGADGA 300

Query: 301 DGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMK 360
           DGG  ENDYEVNKE+VGAS+G V SSEKLSWGKGGIRSHTRYGGINGG GK SRNNRI K
Sbjct: 301 DGGSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNNRITK 360

Query: 361 LSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETALP 420
           LSDYVRNSEN+SEEELD+ L+LVSMD+TRIPAL+  YICCRPSVTIGHL Q VALETAL 
Sbjct: 361 LSDYVRNSENISEEELDVQLMLVSMDKTRIPALRSSYICCRPSVTIGHLRQLVALETALS 420

Query: 421 VDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLVCPE 479
           VDV+EM + KELQMK+NPSTSGT  +PCKES QILN+QETLS  KLK HRLACGYLVC E
Sbjct: 421 VDVIEMYVVKELQMKINPSTSGTFLDPCKESAQILNQQETLSMDKLKTHRLACGYLVCLE 436

BLAST of Lsi01G003010.1 vs. ExPASy TrEMBL
Match: A0A5D3CNZ3 (Putative E3 ubiquitin-protein ligase RING1a OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007610 PE=4 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 2.4e-188
Identity = 377/511 (73.78%), Postives = 405/511 (79.26%), Query Frame = 0

Query: 1   MPARKRAYETVNVDLL-PRLHNTF-NECSDDGESEESDRSPSSSQGDKDE--SCCDRIRR 60
           MPARKRAY++++VDLL PRLHNTF NE S D +S+ESDRS SSSQGD+DE       +  
Sbjct: 1   MPARKRAYDSLDVDLLPPRLHNTFNNERSQDEQSQESDRSSSSSQGDEDEYVPFMSSVLS 60

Query: 61  PAVWASRAEAFVDFCPAPEFSLLSSFEF--------------LYRLWLVELGAGSCGRSG 120
             ++       V F     F L     +              L+ +  VELGAGSCGRS 
Sbjct: 61  LLLFVRLLFRVVPFYSIYSFDLFRLVGYGVSFVANCLGLIWDLHGVPRVELGAGSCGRSE 120

Query: 121 FSR-----------------FIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECI 180
           FSR                 FIAVKLSDIR+EVQCPICLGII+KTRTVMECLHRFCRECI
Sbjct: 121 FSRLYVRSICFYICYELLGGFIAVKLSDIRKEVQCPICLGIIKKTRTVMECLHRFCRECI 180

Query: 181 DKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE------------- 240
           DKSMRLGN ECPACRTHCASRRSLRDDPNYDTLIAVLYPDI+KYEEE             
Sbjct: 181 DKSMRLGNKECPACRTHCASRRSLRDDPNYDTLIAVLYPDIEKYEEEELAFQEEEKARNK 240

Query: 241 -IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKSFQNHIESLCFDENEDENDYDVSKNS 300
            IQTSIAQTLQRQTE+LGRKRSKPSRRLSSRG KSFQNHIESLCFDENEDENDYDVSKNS
Sbjct: 241 QIQTSIAQTLQRQTESLGRKRSKPSRRLSSRGPKSFQNHIESLCFDENEDENDYDVSKNS 300

Query: 301 SSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGSS 360
           SSADERMDTRPKR RRGGPVRFSQSSSATGADGAD GGIE+DY      VGAS+G VGSS
Sbjct: 301 SSADERMDTRPKRPRRGGPVRFSQSSSATGADGADVGGIEHDY------VGASLGLVGSS 360

Query: 361 EKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSMD 420
           EKLSWGKGGIRSHTRYGG NGGAGKNSRNNRI KLSDYVRNSENVSEEELDIH++LVSMD
Sbjct: 361 EKLSWGKGGIRSHTRYGGTNGGAGKNSRNNRIAKLSDYVRNSENVSEEELDIHVLLVSMD 420

Query: 421 ETRIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAKELQMKLNPSTSGTLKN 463
            + IPALQRPYICCRPSV IGHLSQYVALETAL VDVVE+C+A ELQMKL+PSTS  +KN
Sbjct: 421 RS-IPALQRPYICCRPSVMIGHLSQYVALETALSVDVVEICVANELQMKLDPSTSEAIKN 480

BLAST of Lsi01G003010.1 vs. ExPASy TrEMBL
Match: A0A0A0KLI2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517990 PE=4 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 8.5e-186
Identity = 363/478 (75.94%), Postives = 386/478 (80.75%), Query Frame = 0

Query: 1   MPARKRAYETVNVDLL-PRLHNTF-NECSDDGESEESDRSPSSSQGDKDESCCDRIRRPA 60
           MPARKRAY++++VDLL PRLHNTF NE S D +S+ESDRS SSSQGD+DE          
Sbjct: 1   MPARKRAYDSLDVDLLPPRLHNTFNNERSQDEQSQESDRSSSSSQGDEDE---------- 60

Query: 61  VWASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREV 120
                                                          FIAVKLSDIR+EV
Sbjct: 61  -----------------------------------------------FIAVKLSDIRKEV 120

Query: 121 QCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTL 180
           QCPICLGII+KTRTVMECLHRFCRECIDKSMRLGN ECPACRTHCASRRSLRDDPNYDTL
Sbjct: 121 QCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNYDTL 180

Query: 181 IAVLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGR 240
           IAVLYPDI+KYEEE              IQTSIAQTLQRQTE+LGRKRSKPSRRLSSRG 
Sbjct: 181 IAVLYPDIEKYEEEELAFQEEEKARNKQIQTSIAQTLQRQTESLGRKRSKPSRRLSSRGP 240

Query: 241 KSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKRSRRGGPVRFSQSSSATGADG 300
           KSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKR RR GPVRFSQSSSATGADG
Sbjct: 241 KSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKRPRRCGPVRFSQSSSATGADG 300

Query: 301 ADGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIM 360
           ADGGGIE++YEVNKEKVGAS+G VGSSEKLSWGKGGIRSHTRYGG NGGAGK SRNNRI 
Sbjct: 301 ADGGGIEHEYEVNKEKVGASLGLVGSSEKLSWGKGGIRSHTRYGGTNGGAGKISRNNRIA 360

Query: 361 KLSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETAL 420
           KLSDYVRNSENVSEEELDIH++LVSMD T IPALQRPYICCRPSV IGHLSQYVALET+L
Sbjct: 361 KLSDYVRNSENVSEEELDIHVLLVSMDRT-IPALQRPYICCRPSVMIGHLSQYVALETSL 420

Query: 421 PVDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLV 463
            VDVVE+C+AKELQ+KL+PSTS   KNPCKES+QILNEQETLSTAKLKAHRLACGYL+
Sbjct: 421 SVDVVEICVAKELQVKLDPSTSEATKNPCKESVQILNEQETLSTAKLKAHRLACGYLL 420

BLAST of Lsi01G003010.1 vs. ExPASy TrEMBL
Match: A0A6J1IQZ1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478584 PE=4 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 7.2e-185
Identity = 362/494 (73.28%), Postives = 385/494 (77.94%), Query Frame = 0

Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVW 60
           MPARKR YETV+ DLLPRLH+TFN+ S+DGESEESDRS SSSQ D+DE            
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSSQEDEDE------------ 60

Query: 61  ASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQC 120
                                                        F+AVKLSDIRR+VQC
Sbjct: 61  ---------------------------------------------FVAVKLSDIRRDVQC 120

Query: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIA 180
           PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIA
Sbjct: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIA 180

Query: 181 VLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKS 240
           VLYPDIDKYEEE              IQ+SIAQTL+RQTEALGRKRSKP RRLSSRG+KS
Sbjct: 181 VLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGQKS 240

Query: 241 FQNHIESLCFDENEDENDYDVSKNSSSADERMDTRP-KRSRRGGPVRFSQSSSATGADGA 300
           FQNHIESL FDENEDENDYD SKNSSSADERMDTRP KRSRRGGPVRFSQSSSAT   GA
Sbjct: 241 FQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSAT---GA 300

Query: 301 DGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMK 360
           DGGG END EVNKEKVGAS+G V SSEK SWGKGGIRSHTRYGGINGG GK SRNNRI K
Sbjct: 301 DGGGNENDCEVNKEKVGASMGLVSSSEKHSWGKGGIRSHTRYGGINGGGGKISRNNRITK 360

Query: 361 LSDYVRNSENVS-EEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETAL 420
           LSDYVRNSEN+S EEELD+HL+LVSMD+TRIPAL+ PYICCRPSVTIGHL Q VAL TAL
Sbjct: 361 LSDYVRNSENISEEEELDVHLMLVSMDKTRIPALRSPYICCRPSVTIGHLRQLVALVTAL 420

Query: 421 PVDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLVCP 479
            VDV+EM + KELQMK+NPSTSG   +PCKES QILN+QETLS  KLK HRLACGYLVC 
Sbjct: 421 SVDVIEMYVVKELQMKINPSTSGAFLDPCKESAQILNQQETLSMDKLKTHRLACGYLVCL 434

BLAST of Lsi01G003010.1 vs. ExPASy TrEMBL
Match: A0A6J1ECT6 (putative E3 ubiquitin-protein ligase RING1a isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433005 PE=4 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 1.5e-182
Identity = 354/477 (74.21%), Postives = 376/477 (78.83%), Query Frame = 0

Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVW 60
           MPARKR YETV+ DLLPRLH+TFN+ S+DGESEESDRS SSSQ DKDE            
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSSQEDKDE------------ 60

Query: 61  ASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQC 120
                                                        F+AVKLSDIRR+VQC
Sbjct: 61  ---------------------------------------------FVAVKLSDIRRDVQC 120

Query: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIA 180
           PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIA
Sbjct: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIA 180

Query: 181 VLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKS 240
           VLYPDIDKYEEE              IQ+SIAQTL+RQTEALGRKRSKP RRLSSRGRKS
Sbjct: 181 VLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGRKS 240

Query: 241 FQNHIESLCFDENEDENDYDVSKNSSSADERMDTRP-KRSRRGGPVRFSQSSSATGADGA 300
           FQNHIESL FDENEDENDYD SKNSSSADERMDTRP KRSRRGGPVRFSQSSSATGADGA
Sbjct: 241 FQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGADGA 300

Query: 301 DGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMK 360
           DGG  ENDYEVNKE+VGAS+G V SSEKLSWGKGGIRSHTRYGGINGG GK SRNNRI K
Sbjct: 301 DGGSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNNRITK 360

Query: 361 LSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETALP 420
           LSDYVRNSEN+SEEELD+ L+LVSMD+TRIPAL+  YICCRPSVTIGHL Q VALETAL 
Sbjct: 361 LSDYVRNSENISEEELDVQLMLVSMDKTRIPALRSSYICCRPSVTIGHLRQLVALETALS 420

Query: 421 VDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLV 463
           VDV+EM + KELQMK+NPSTSGT  +PCKES QILN+QETLS  KLK HRLACGYL+
Sbjct: 421 VDVIEMYVVKELQMKINPSTSGTFLDPCKESAQILNQQETLSMDKLKTHRLACGYLL 420

BLAST of Lsi01G003010.1 vs. NCBI nr
Match: XP_038881714.1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 [Benincasa hispida])

HSP 1 Score: 699.5 bits (1804), Expect = 2.0e-197
Identity = 376/476 (78.99%), Postives = 392/476 (82.35%), Query Frame = 0

Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVW 60
           MPARKRAY+TV+VDLLPRLHNTFNECS+DG+SEESDRSPSSSQGDKDE            
Sbjct: 1   MPARKRAYDTVDVDLLPRLHNTFNECSEDGQSEESDRSPSSSQGDKDE------------ 60

Query: 61  ASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQC 120
                                                        FIAVKLSDIR+EVQC
Sbjct: 61  ---------------------------------------------FIAVKLSDIRKEVQC 120

Query: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIA 180
           PICLGII+KTRTVMECLHRFCRECIDKSMRLGN ECPACRTHCASRRSLRDDPNYDTLIA
Sbjct: 121 PICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNYDTLIA 180

Query: 181 VLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKS 240
           VLYPDIDKYEEE              IQTSIAQTLQRQTEALGRKRSKPSRRLSSRG KS
Sbjct: 181 VLYPDIDKYEEEELAFQEEEKGRNKQIQTSIAQTLQRQTEALGRKRSKPSRRLSSRGSKS 240

Query: 241 FQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKRSRRGGPVRFSQSSSATGADGAD 300
           FQNHIES+CFDENEDENDYDVSKNSSSADERMD RPKRSRRGGPVRFSQSSSATGADG D
Sbjct: 241 FQNHIESICFDENEDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATGADGTD 300

Query: 301 GGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKL 360
            GGIENDYEV KEKVGASIG VGSSEKLSWGKGGIRSHTRYGG+NGGAGKN RNNRIMKL
Sbjct: 301 -GGIENDYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNNRIMKL 360

Query: 361 SDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETALPV 420
           SDYVRNSENVSE+ELDIHLVL+S+DETR+PALQRPYI CRPSVTIGHLSQYVAL+T L V
Sbjct: 361 SDYVRNSENVSEKELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALKTGLSV 418

Query: 421 DVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLV 463
           DVVEMC+AKELQMKLNPS SGTLKNPCKE IQILNEQETLSTAK+KAHRLACGYL+
Sbjct: 421 DVVEMCVAKELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYLL 418

BLAST of Lsi01G003010.1 vs. NCBI nr
Match: XP_023543673.1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 679.5 bits (1752), Expect = 2.1e-191
Identity = 367/493 (74.44%), Postives = 389/493 (78.90%), Query Frame = 0

Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVW 60
           MPARKR YETV+ DLLPRLH+TFN+ S+DGESEESDRS SS Q DKDE            
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSGQEDKDE------------ 60

Query: 61  ASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQC 120
                                                        F+AVKLSDIRR+VQC
Sbjct: 61  ---------------------------------------------FVAVKLSDIRRDVQC 120

Query: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIA 180
           PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIA
Sbjct: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIA 180

Query: 181 VLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKS 240
           VLYPDIDKYEEE              IQ+SIAQTL+RQTEALGRKRSKP RRLSSRGRKS
Sbjct: 181 VLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGRKS 240

Query: 241 FQNHIESLCFDENEDENDYDVSKNSSSADERMDTRP-KRSRRGGPVRFSQSSSATGADGA 300
           FQNHIESL FDENEDENDYD SKNSSSADERMDTRP KRSRRGGPVRFSQSSSATGADGA
Sbjct: 241 FQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGADGA 300

Query: 301 DGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMK 360
           DGG  ENDYEVNKE+VGAS+G V SSEKLSWGKGGIRSHTRYGGINGG GK SRNNRI K
Sbjct: 301 DGGSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNNRITK 360

Query: 361 LSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETALP 420
           LSDYVRNSEN+SEEELD+HL+LVSMD+TRIPAL+ PYICCRPSVTIGHL Q VALETAL 
Sbjct: 361 LSDYVRNSENISEEELDVHLMLVSMDKTRIPALRSPYICCRPSVTIGHLRQLVALETALS 420

Query: 421 VDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLVCPE 479
           VDV+EM + KELQMK+NPSTSGT  +PCKES QILN+QETLS  KLK HRLACGYLVC E
Sbjct: 421 VDVIEMYVVKELQMKINPSTSGTFLDPCKESAQILNQQETLSMDKLKTHRLACGYLVCLE 436

BLAST of Lsi01G003010.1 vs. NCBI nr
Match: XP_022925653.1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 [Cucurbita moschata])

HSP 1 Score: 674.9 bits (1740), Expect = 5.3e-190
Identity = 366/493 (74.24%), Postives = 388/493 (78.70%), Query Frame = 0

Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVW 60
           MPARKR YETV+ DLLPRLH+TFN+ S+DGESEESDRS SSSQ DKDE            
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSSQEDKDE------------ 60

Query: 61  ASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQC 120
                                                        F+AVKLSDIRR+VQC
Sbjct: 61  ---------------------------------------------FVAVKLSDIRRDVQC 120

Query: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIA 180
           PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIA
Sbjct: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIA 180

Query: 181 VLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKS 240
           VLYPDIDKYEEE              IQ+SIAQTL+RQTEALGRKRSKP RRLSSRGRKS
Sbjct: 181 VLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGRKS 240

Query: 241 FQNHIESLCFDENEDENDYDVSKNSSSADERMDTRP-KRSRRGGPVRFSQSSSATGADGA 300
           FQNHIESL FDENEDENDYD SKNSSSADERMDTRP KRSRRGGPVRFSQSSSATGADGA
Sbjct: 241 FQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGADGA 300

Query: 301 DGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMK 360
           DGG  ENDYEVNKE+VGAS+G V SSEKLSWGKGGIRSHTRYGGINGG GK SRNNRI K
Sbjct: 301 DGGSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNNRITK 360

Query: 361 LSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETALP 420
           LSDYVRNSEN+SEEELD+ L+LVSMD+TRIPAL+  YICCRPSVTIGHL Q VALETAL 
Sbjct: 361 LSDYVRNSENISEEELDVQLMLVSMDKTRIPALRSSYICCRPSVTIGHLRQLVALETALS 420

Query: 421 VDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLVCPE 479
           VDV+EM + KELQMK+NPSTSGT  +PCKES QILN+QETLS  KLK HRLACGYLVC E
Sbjct: 421 VDVIEMYVVKELQMKINPSTSGTFLDPCKESAQILNQQETLSMDKLKTHRLACGYLVCLE 436

BLAST of Lsi01G003010.1 vs. NCBI nr
Match: KAG7034603.1 (putative E3 ubiquitin-protein ligase RING1a, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 671.4 bits (1731), Expect = 5.8e-189
Identity = 379/541 (70.06%), Postives = 404/541 (74.68%), Query Frame = 0

Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDE------------ 60
           MPARKR YETV+ DLLPRLH+TFN+ S+DGESEESDRS SSSQ DKDE            
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSSQEDKDEYVPFMMSSVFGL 60

Query: 61  ------------------------------------SC------------CDRIRRPAVW 120
                                                C               + RP+  
Sbjct: 61  LLFVRHLLRAVPFYSIFSFDLFHLVGYGVLWCLTAWDCFGICMVCFVVLFSKFLLRPSSK 120

Query: 121 AS----RAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRR 180
            S     +EAFV F PAPE              LVELGAGSC     SRF+AVKLSDIRR
Sbjct: 121 TSCVVFTSEAFVGFYPAPE--------------LVELGAGSC---WLSRFVAVKLSDIRR 180

Query: 181 EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 240
           +VQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+D
Sbjct: 181 DVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFD 240

Query: 241 TLIAVLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSR 300
           TLIAVLYPDIDKYEEE              IQ+SIAQTL+RQTEALGRKRSKP RRLSSR
Sbjct: 241 TLIAVLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSR 300

Query: 301 GRKSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRP-KRSRRGGPVRFSQSSSATG 360
           GRKSFQNHIESL FDENEDENDYD SKNSSSADERMDTRP KRSRRGGP+RFSQSSSATG
Sbjct: 301 GRKSFQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPIRFSQSSSATG 360

Query: 361 ADGADGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNN 420
           ADGADGG  ENDYEVNKE+VGAS+G V SSEKLSWGKGGIRSHTRYGGINGG GK SRNN
Sbjct: 361 ADGADGGSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNN 420

Query: 421 RIMKLSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALE 463
           RI KLSDYVRNSEN+SEEELD+ L+LVSMD+TRIPAL+ PYICCRPSVTIGHL Q VALE
Sbjct: 421 RITKLSDYVRNSENISEEELDVQLMLVSMDKTRIPALRSPYICCRPSVTIGHLRQLVALE 480

BLAST of Lsi01G003010.1 vs. NCBI nr
Match: TYK13150.1 (putative E3 ubiquitin-protein ligase RING1a [Cucumis melo var. makuwa])

HSP 1 Score: 668.3 bits (1723), Expect = 4.9e-188
Identity = 377/511 (73.78%), Postives = 405/511 (79.26%), Query Frame = 0

Query: 1   MPARKRAYETVNVDLL-PRLHNTF-NECSDDGESEESDRSPSSSQGDKDE--SCCDRIRR 60
           MPARKRAY++++VDLL PRLHNTF NE S D +S+ESDRS SSSQGD+DE       +  
Sbjct: 1   MPARKRAYDSLDVDLLPPRLHNTFNNERSQDEQSQESDRSSSSSQGDEDEYVPFMSSVLS 60

Query: 61  PAVWASRAEAFVDFCPAPEFSLLSSFEF--------------LYRLWLVELGAGSCGRSG 120
             ++       V F     F L     +              L+ +  VELGAGSCGRS 
Sbjct: 61  LLLFVRLLFRVVPFYSIYSFDLFRLVGYGVSFVANCLGLIWDLHGVPRVELGAGSCGRSE 120

Query: 121 FSR-----------------FIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECI 180
           FSR                 FIAVKLSDIR+EVQCPICLGII+KTRTVMECLHRFCRECI
Sbjct: 121 FSRLYVRSICFYICYELLGGFIAVKLSDIRKEVQCPICLGIIKKTRTVMECLHRFCRECI 180

Query: 181 DKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE------------- 240
           DKSMRLGN ECPACRTHCASRRSLRDDPNYDTLIAVLYPDI+KYEEE             
Sbjct: 181 DKSMRLGNKECPACRTHCASRRSLRDDPNYDTLIAVLYPDIEKYEEEELAFQEEEKARNK 240

Query: 241 -IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKSFQNHIESLCFDENEDENDYDVSKNS 300
            IQTSIAQTLQRQTE+LGRKRSKPSRRLSSRG KSFQNHIESLCFDENEDENDYDVSKNS
Sbjct: 241 QIQTSIAQTLQRQTESLGRKRSKPSRRLSSRGPKSFQNHIESLCFDENEDENDYDVSKNS 300

Query: 301 SSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGSS 360
           SSADERMDTRPKR RRGGPVRFSQSSSATGADGAD GGIE+DY      VGAS+G VGSS
Sbjct: 301 SSADERMDTRPKRPRRGGPVRFSQSSSATGADGADVGGIEHDY------VGASLGLVGSS 360

Query: 361 EKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSMD 420
           EKLSWGKGGIRSHTRYGG NGGAGKNSRNNRI KLSDYVRNSENVSEEELDIH++LVSMD
Sbjct: 361 EKLSWGKGGIRSHTRYGGTNGGAGKNSRNNRIAKLSDYVRNSENVSEEELDIHVLLVSMD 420

Query: 421 ETRIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAKELQMKLNPSTSGTLKN 463
            + IPALQRPYICCRPSV IGHLSQYVALETAL VDVVE+C+A ELQMKL+PSTS  +KN
Sbjct: 421 RS-IPALQRPYICCRPSVMIGHLSQYVALETALSVDVVEICVANELQMKLDPSTSEAIKN 480

BLAST of Lsi01G003010.1 vs. TAIR 10
Match: AT5G44280.1 (RING 1A )

HSP 1 Score: 283.9 bits (725), Expect = 2.4e-76
Identity = 171/383 (44.65%), Postives = 237/383 (61.88%), Query Frame = 0

Query: 104 SRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHC 163
           S F+ + L +IR++VQCPICLGII+KTRTVMECLHRFCRECIDKSMRLGNNECPACR HC
Sbjct: 120 SEFMEIDLGEIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRKHC 179

Query: 164 ASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQTLQRQTEALG 223
           ASRRSLRDDP +D LIA L+ +ID YEEE              IQ SIAQ  QRQ+EAL 
Sbjct: 180 ASRRSLRDDPKFDALIAALFTNIDSYEEEELAFHEDEMARNKQIQASIAQISQRQSEALV 239

Query: 224 RKRS---------------KPSRRLSSRGRKSFQNHIESLCFDENEDENDYDVSKNSSSA 283
           ++RS                 SRR  +      QN  E+   D+N+D N+    K+SSS 
Sbjct: 240 KRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQQNASEAHEDDDNDDNNNRGRDKDSSSD 299

Query: 284 DERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGSSEKL 343
           +   + R K+ R+    R +Q  S++GA+  +G   +ND EV ++  G S G V + E L
Sbjct: 300 ERGTEVRQKKRRKRSTSRSTQHPSSSGANKNNGNCADNDTEVYRDTKGISPGLVWNPEIL 359

Query: 344 SWGKGGIRSHTRYG-GINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSMDET 403
           +WG+GG RS+TR+G   +GG+ K+ RN R+ KL +Y+R+S + S  ELDIH+ LVS+D  
Sbjct: 360 AWGRGGTRSNTRHGNNTSGGSSKSVRNARVNKLVEYLRSSVDGSSVELDIHVKLVSLDTK 419

Query: 404 RIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAK-----ELQMKLNPSTSGT 452
            IP L +PY+CCRP++ +  L ++VAL+  L  + VE+ + +     +  ++  P+ +  
Sbjct: 420 CIPDLPQPYLCCRPTLLVKQLREFVALQIHLKTEEVELLVTRRRVGEDAAIENLPAVTPA 479

BLAST of Lsi01G003010.1 vs. TAIR 10
Match: AT1G03770.1 (RING 1B )

HSP 1 Score: 245.0 bits (624), Expect = 1.3e-64
Identity = 168/393 (42.75%), Postives = 230/393 (58.52%), Query Frame = 0

Query: 95  AGSCGRSGFSRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 154
           + S G    S ++ V L+DI ++VQC ICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN
Sbjct: 78  SSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 137

Query: 155 ECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQT 214
           ECP CR HCASRRSLRDDPN+D LIA L+ +IDK+EEE              IQ SIAQ 
Sbjct: 138 ECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQIQASIAQV 197

Query: 215 LQRQTEALGRKRS--------KPSRRL--SSRGRKSFQNHIESLCFDENEDENDYDVSKN 274
            QRQ++AL +++S          SRR    SR R++ +N IE    + N+D++     K+
Sbjct: 198 SQRQSKALVKRKSVGKGTAILSRSRRSGGGSRRRRNCRN-IEQDTSEANDDDDQNKRGKD 257

Query: 275 SSSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGS 334
           SSS +     R KRS          SS+A   D   G G E  ++ +   +   +  V +
Sbjct: 258 SSSDEPCERQRKKRS-----ATQPSSSNANNNDNCAGNGTEQTHQRDSRVISPVL--VWN 317

Query: 335 SEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSM 394
           SE ++WG+GG RS+TR G  N GA  + RN R+ +L +Y+ + E  +  ELDIHL LVS+
Sbjct: 318 SELIAWGRGGTRSNTRQGNNNQGA-ISKRNARLKRLVEYLGSLEG-NSVELDIHLKLVSL 377

Query: 395 DETRIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAKELQMKLNPSTSGTLK 454
           D   +  L  PY+C RP++ +  L +YVA    L  + VE+ ++K+    +   TS    
Sbjct: 378 DTEGLLNLHEPYLCFRPTLLVKQLREYVARHLKLKAEEVELLVSKDGDTVIGNKTS---- 437

Query: 455 NPCKESIQILNEQETLSTAKLKAHRLAC-GYLV 463
               E +Q L + ET+  AKLK   ++  GY++
Sbjct: 438 ---TEKMQSLQDDETV--AKLKVDCISSNGYMI 451

BLAST of Lsi01G003010.1 vs. TAIR 10
Match: AT1G03770.3 (RING 1B )

HSP 1 Score: 238.4 bits (607), Expect = 1.2e-62
Identity = 168/399 (42.11%), Postives = 230/399 (57.64%), Query Frame = 0

Query: 95  AGSCGRSGFSRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 154
           + S G    S ++ V L+DI ++VQC ICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN
Sbjct: 78  SSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 137

Query: 155 ECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQT 214
           ECP CR HCASRRSLRDDPN+D LIA L+ +IDK+EEE              IQ SIAQ 
Sbjct: 138 ECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQIQASIAQV 197

Query: 215 LQRQTEALGRKRS--------KPSRRL--SSRGRKSFQNHIESLCFDENEDENDYDVSKN 274
            QRQ++AL +++S          SRR    SR R++ +N IE    + N+D++     K+
Sbjct: 198 SQRQSKALVKRKSVGKGTAILSRSRRSGGGSRRRRNCRN-IEQDTSEANDDDDQNKRGKD 257

Query: 275 SSSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGS 334
           SSS +     R KRS          SS+A   D   G G E  ++ +   +   +  V +
Sbjct: 258 SSSDEPCERQRKKRS-----ATQPSSSNANNNDNCAGNGTEQTHQRDSRVISPVL--VWN 317

Query: 335 SEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSM 394
           SE ++WG+GG RS+TR G  N GA  + RN R+ +L +Y+ + E  +  ELDIHL LVS+
Sbjct: 318 SELIAWGRGGTRSNTRQGNNNQGA-ISKRNARLKRLVEYLGSLEG-NSVELDIHLKLVSL 377

Query: 395 DETRIPALQRPYICCRPSVTIGHLSQ------YVALETALPVDVVEMCMAKELQMKLNPS 454
           D   +  L  PY+C RP++ +  L +      YVA    L  + VE+ ++K+    +   
Sbjct: 378 DTEGLLNLHEPYLCFRPTLLVKQLREVSSLPLYVARHLKLKAEEVELLVSKDGDTVIGNK 437

Query: 455 TSGTLKNPCKESIQILNEQETLSTAKLKAHRLAC-GYLV 463
           TS        E +Q L + ET+  AKLK   ++  GY++
Sbjct: 438 TS-------TEKMQSLQDDETV--AKLKVDCISSNGYMI 457

BLAST of Lsi01G003010.1 vs. TAIR 10
Match: AT1G03770.2 (RING 1B )

HSP 1 Score: 238.4 bits (607), Expect = 1.2e-62
Identity = 168/399 (42.11%), Postives = 230/399 (57.64%), Query Frame = 0

Query: 95  AGSCGRSGFSRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 154
           + S G    S ++ V L+DI ++VQC ICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN
Sbjct: 80  SSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 139

Query: 155 ECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQT 214
           ECP CR HCASRRSLRDDPN+D LIA L+ +IDK+EEE              IQ SIAQ 
Sbjct: 140 ECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQIQASIAQV 199

Query: 215 LQRQTEALGRKRS--------KPSRRL--SSRGRKSFQNHIESLCFDENEDENDYDVSKN 274
            QRQ++AL +++S          SRR    SR R++ +N IE    + N+D++     K+
Sbjct: 200 SQRQSKALVKRKSVGKGTAILSRSRRSGGGSRRRRNCRN-IEQDTSEANDDDDQNKRGKD 259

Query: 275 SSSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGS 334
           SSS +     R KRS          SS+A   D   G G E  ++ +   +   +  V +
Sbjct: 260 SSSDEPCERQRKKRS-----ATQPSSSNANNNDNCAGNGTEQTHQRDSRVISPVL--VWN 319

Query: 335 SEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSM 394
           SE ++WG+GG RS+TR G  N GA  + RN R+ +L +Y+ + E  +  ELDIHL LVS+
Sbjct: 320 SELIAWGRGGTRSNTRQGNNNQGA-ISKRNARLKRLVEYLGSLEG-NSVELDIHLKLVSL 379

Query: 395 DETRIPALQRPYICCRPSVTIGHLSQ------YVALETALPVDVVEMCMAKELQMKLNPS 454
           D   +  L  PY+C RP++ +  L +      YVA    L  + VE+ ++K+    +   
Sbjct: 380 DTEGLLNLHEPYLCFRPTLLVKQLREVSSLPLYVARHLKLKAEEVELLVSKDGDTVIGNK 439

Query: 455 TSGTLKNPCKESIQILNEQETLSTAKLKAHRLAC-GYLV 463
           TS        E +Q L + ET+  AKLK   ++  GY++
Sbjct: 440 TS-------TEKMQSLQDDETV--AKLKVDCISSNGYMI 459

BLAST of Lsi01G003010.1 vs. TAIR 10
Match: AT5G44280.2 (RING 1A )

HSP 1 Score: 217.2 bits (552), Expect = 2.8e-56
Identity = 147/387 (37.98%), Postives = 220/387 (56.85%), Query Frame = 0

Query: 104 SRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMR----LGNNECPAC 163
           S F+ + L +IR++VQCPICL  ++  R  +   ++  ++C   S +      NNECPAC
Sbjct: 120 SEFMEIDLGEIRKDVQCPICLVGMKFLRLFIS-YYKENKDCDGMSPQFCATFKNNECPAC 179

Query: 164 RTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQTLQRQT 223
           R HCASRRSLRDDP +D LIA L+ +ID YEEE              IQ SIAQ  QRQ+
Sbjct: 180 RKHCASRRSLRDDPKFDALIAALFTNIDSYEEEELAFHEDEMARNKQIQASIAQISQRQS 239

Query: 224 EALGRKRS---------------KPSRRLSSRGRKSFQNHIESLCFDENEDENDYDVSKN 283
           EAL ++RS                 SRR  +      QN  E+   D+N+D N+    K+
Sbjct: 240 EALVKRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQQNASEAHEDDDNDDNNNRGRDKD 299

Query: 284 SSSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGS 343
           SSS +   + R K+ R+    R +Q  S++GA+  +G   +ND EV ++  G S G V +
Sbjct: 300 SSSDERGTEVRQKKRRKRSTSRSTQHPSSSGANKNNGNCADNDTEVYRDTKGISPGLVWN 359

Query: 344 SEKLSWGKGGIRSHTRYG-GINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVS 403
            E L+WG+GG RS+TR+G   +GG+ K+ RN R+ KL +Y+R+S + S  ELDIH+ LVS
Sbjct: 360 PEILAWGRGGTRSNTRHGNNTSGGSSKSVRNARVNKLVEYLRSSVDGSSVELDIHVKLVS 419

Query: 404 MDETRIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAK-----ELQMKLNPS 452
           +D   IP L +PY+CCRP++ +  L ++VAL+  L  + VE+ + +     +  ++  P+
Sbjct: 420 LDTKCIPDLPQPYLCCRPTLLVKQLREFVALQIHLKTEEVELLVTRRRVGEDAAIENLPA 479

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FKW03.4e-7544.65Putative E3 ubiquitin-protein ligase RING1a OS=Arabidopsis thaliana OX=3702 GN=R... [more]
Q0WX001.8e-6342.75Putative E3 ubiquitin-protein ligase RING1b OS=Arabidopsis thaliana OX=3702 GN=R... [more]
Q5TJF39.2e-2033.50E3 ubiquitin-protein ligase RING1 OS=Canis lupus familiaris OX=9615 GN=RING1 PE=... [more]
A1YER59.2e-2033.50E3 ubiquitin-protein ligase RING1 OS=Gorilla gorilla gorilla OX=9595 GN=RING1 PE... [more]
Q065872.7e-1932.99E3 ubiquitin-protein ligase RING1 OS=Homo sapiens OX=9606 GN=RING1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1EFU82.5e-19074.24putative E3 ubiquitin-protein ligase RING1a isoform X1 OS=Cucurbita moschata OX=... [more]
A0A5D3CNZ32.4e-18873.78Putative E3 ubiquitin-protein ligase RING1a OS=Cucumis melo var. makuwa OX=11946... [more]
A0A0A0KLI28.5e-18675.94RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517990 P... [more]
A0A6J1IQZ17.2e-18573.28putative E3 ubiquitin-protein ligase RING1a isoform X1 OS=Cucurbita maxima OX=36... [more]
A0A6J1ECT61.5e-18274.21putative E3 ubiquitin-protein ligase RING1a isoform X2 OS=Cucurbita moschata OX=... [more]
Match NameE-valueIdentityDescription
XP_038881714.12.0e-19778.99putative E3 ubiquitin-protein ligase RING1a isoform X1 [Benincasa hispida][more]
XP_023543673.12.1e-19174.44putative E3 ubiquitin-protein ligase RING1a isoform X1 [Cucurbita pepo subsp. pe... [more]
XP_022925653.15.3e-19074.24putative E3 ubiquitin-protein ligase RING1a isoform X1 [Cucurbita moschata][more]
KAG7034603.15.8e-18970.06putative E3 ubiquitin-protein ligase RING1a, partial [Cucurbita argyrosperma sub... [more]
TYK13150.14.9e-18873.78putative E3 ubiquitin-protein ligase RING1a [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT5G44280.12.4e-7644.65RING 1A [more]
AT1G03770.11.3e-6442.75RING 1B [more]
AT1G03770.31.2e-6242.11RING 1B [more]
AT1G03770.21.2e-6242.11RING 1B [more]
AT5G44280.22.8e-5637.98RING 1A [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 120..159
e-value: 9.7E-7
score: 38.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 120..160
score: 12.076194
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 104..208
e-value: 2.9E-18
score: 67.7
NoneNo IPR availablePFAMPF13923zf-C3HC4_2coord: 119..159
e-value: 9.8E-12
score: 44.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 204..226
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 24..49
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 245..266
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 241..292
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 33..49
NoneNo IPR availablePANTHERPTHR46537:SF3OS11G0578200 PROTEINcoord: 1..452
NoneNo IPR availableCDDcd16531RING-HC_RING1_likecoord: 120..160
e-value: 4.21225E-21
score: 84.0207
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 111..192
IPR044592Putative E3 ubiquitin-protein ligase RING1a/bPANTHERPTHR46537OS11G0578200 PROTEINcoord: 1..452
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 136..145

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lsi01G003010Lsi01G003010gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi01G003010.1.exon.9Lsi01G003010.1.exon.9exon
Lsi01G003010.1.exon.8Lsi01G003010.1.exon.8exon
Lsi01G003010.1.exon.7Lsi01G003010.1.exon.7exon
Lsi01G003010.1.exon.6Lsi01G003010.1.exon.6exon
Lsi01G003010.1.exon.5Lsi01G003010.1.exon.5exon
Lsi01G003010.1.exon.4Lsi01G003010.1.exon.4exon
Lsi01G003010.1.exon.3Lsi01G003010.1.exon.3exon
Lsi01G003010.1.exon.2Lsi01G003010.1.exon.2exon
Lsi01G003010.1.exon.1Lsi01G003010.1.exon.1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi01G003010.1.CDS.9Lsi01G003010.1.CDS.9CDS
Lsi01G003010.1.CDS.8Lsi01G003010.1.CDS.8CDS
Lsi01G003010.1.CDS.7Lsi01G003010.1.CDS.7CDS
Lsi01G003010.1.CDS.6Lsi01G003010.1.CDS.6CDS
Lsi01G003010.1.CDS.5Lsi01G003010.1.CDS.5CDS
Lsi01G003010.1.CDS.4Lsi01G003010.1.CDS.4CDS
Lsi01G003010.1.CDS.3Lsi01G003010.1.CDS.3CDS
Lsi01G003010.1.CDS.2Lsi01G003010.1.CDS.2CDS
Lsi01G003010.1.CDS.1Lsi01G003010.1.CDS.1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi01G003010.1.five_prime_UTR.1Lsi01G003010.1.five_prime_UTR.1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lsi01G003010.1Lsi01G003010.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding