Lsi01G003010.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi01G003010.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	RING-type domain-containing protein
Location	chr01: 2689575 .. 2694329 (-)
Sequence length	1635
RNA-Seq Expression	Lsi01G003010.1
Synteny	Lsi01G003010.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTGGTGTGTTTTTCTTTAATCAATCTAATGCCGTGGAAATTTCGTAAAACCACGAACACGAGGCTCCTTCCCTCTCGGCTCTGATATTTTGCTCCACCGCCCCCTTCATCATCTCTACGAACCTCAATTTTTGTGAACTTACAACTTCTTTTCGTTGTTTCTTCAACTTATTTTGCTTCAGACCGAAGCAATGCCGGCTCGGAAGCGCGCTTACGAGACTGTGAATGTTGATCTTTTGCCGCGACTCCATAATACCTTCAATGAATGCTCTGACGATGGAGAATCTGAGGGTACGCTTCTTGTTTCTCTTCAGCACCCTAAATTTTCACGATGCTACCGACTCTTTGGTGTTCCTGCATTTCATATATGTTATTATTTGTTTGTTTAATTCTTTTTTTTGTGCGTCTACTGAGTTCGTGGTGTTCTATGTTGCTCCTTCTTTCGCCAGAATCCGATCGAAGCCCTTCGTCCAGTCAAGGAGATAAGGACGAGTATGTTCCGTTTATGAACTCTGTTTTTCGCTTGCTTCTATTTGTTCGTCATCTTCTTCGTGCTCTTCCTTTTTACTCTATCTACTCTTTTGATTTATTTCCTCTGGTTGGTTATGGAGTTCTTTGGTGCTTAACTGCTTGGGATTGATTTGGGATCTGCATGGTATGCTTCGTGGTATTGTTCATCGAGGTCTTGCTGCGACCGTATTCGAAGACCTGCTGTGTGGGCTTCACGTGCTGAAGCATTCGTCGATTTTTGTCCTGCACCGGAGTTTTCTTTACTTTCAAGTTTTGAGTTTCTTTACCGATTATGGTTAGTGGAATTGGGTGCTGGATCTTGTGGGCGAAGTGGGTTCTCGAGGTCGTATATCCGATCAATCTGCTTTTACATATGCTACGAGCTGCTGGGTGGGTAATGACGTTGGTGCTCACATGGTTTTAGCTTCTTTACGATATTAAAGCTTACATGATCCGAGATCAAGCTTTCACTCAACGTGAAATCAAGCTTATTATAACTGAAGCGTTCACGGAAAAATCTTGCTGGTTCCCGCCATTGATTGTCGGATCCAATTTTACGCAAATTCCAAGAAGTTAACGACTGGAAACCCGACATTTCATTAAGAACGATTTTACCCAAATTTTTTACAACTTGAGCGGTACTCTTACGCTAGCGCGATGAACGAACTATTTGGGTTGCGTTATAGAATATATGTATCTGAACAGAAAGTTGTTTATCGGTTCTTTAGTTGCATGGTGAACAGTAGAATTCCCTGAACGATTAACTCGATCTTAGGTGTCTTGCGATTATTCCGACATCCTAAATCTTCTGTCTGGTTTTCTTTTCCAGATTTATCGCCGTGAAATTGTCAGACATTCGGAGAGAAGTTCAATGTCCTATCTGCTTAGGTATGTTTTAATAATCTCTTCTTGTTGAACGTATTTATCTCATATAAGATAAGTTTTCTATTGATTTGAACATTTGGTTTTAATTCTTATACAATTCATGTTCTGCAAGTATATAAACACGCCTACTACTTGAAACGACTTTGTGTTTACCATTGGTGTCAATGTGTCATCCAGGTCTTTACTGACTTTAACAAGTAAGATTGTTCTCATATCCTGAGAATCAATCATCGTGTATAATTAACTATTTTCGTAAGTTTATGTACATACGCACTGCAAGATGACCTATCAATAAGGTTATTTCAAATGATTGATTTGTCCGGGATGAATTGTGGAGGATGAGAGCATTAATTTTAAATGAAGTGTTGAATCAAGTGCCACCGGACTCATCTGATCCAAGAATTTATTCTGATATAACAAATTTGATTTTCTGTATATAAGTTGTATCAAATAACATAAAGATTGTTTGTGATCTCCGGATCTGGGAGTGATGGCAATCATTAAAATCTGAGGTTGACTTTGTATATGGACTATCGATTTAGATATGGATTCTGATATGGCTTCTTTATCAGTTTTTGCTTTGGCTTAAAAAAGTTATTAGTCAGTTGTCACCCCCCCTCCACTTCCAGGGTCTTGCATATAAAACAATTGCATTTTTACGTTCCATGGACCTCTGTTTGTATTTTAGGTGACACTTATAATTGCAATCCTTCTGCTTTTCTTATTCCCCACAAGTAACTTAGAAGTTAAGATGACAAACTTTTCTACCTCCACTTATTTGTCCTAACATCAACTTCGTTATCGAGTTTTGTATTTTTTGATTAGACCATTCCGTTAAAGTAACTAATTGGACCGTAGAGTGTGAATTCATGAGCATATGAGGGTATGGAATTTGGTGATCTGCATTCTTTTTCGAACCTTACCGTTTCAGCCTTTCAGGCTACATAAGTTTATGTGGAGCCAGTAATTTATGATGGTGTTACATGTTTACACATAACAAGTAGTAAATATATGCATGACTTTCAAATTATTGATTACTCAAGCTCTAATTGTCAAGATTATTGATCCTTACTTTCAGTTCATGCAGTATCTGATAAAGGAAACACCCAAAAATCTTCCTTATCATAAGACAATAACTAAATTCCAACTCGAAACATTCCTGAAATTTTACATTTTTTAGTGGATGTATCCAAATACTATGAGCCATGACATGCTAAGTCTTAGCATGTTTGGGAAGTATATTTAGGTGGATTTGGTGAGGCAACCTCAGAGTTCACTTTGGAAGGTCATCTCCATGTTTATGCATGAGTAGTATTCTTAAGGAGGTTGATAAAGTTTTTTCAATTCCTAATGTGCAGGAATCATCAGAAAAACCAGAACAGTGATGGAATGTCTGCACCGCTTCTGCAGGGAATGCATAGACAAATCGATGCGTCTAGGGTATGTTCTTATGTAATTTTTTATTTTGATCTTGCTAATTGCTATACAGCTCAAGAAAATAAATATATATCGCAGCACAACTTTCCTTCTGGCTTCCACTAAACTTTTACTGAAATTTGCTGCAGTAACAATGAGTGCCCTGCTTGCCGCACTCATTGTGCCAGTCGACGCTCTCTGAGAGATGATCCGAATTATGATACATTGATTGCTGTGTTATATCCAGATATTGACAAATATGAGGAAGAGGTGGGAATATTATGTTTAAGCTTGCAAGCTTGGTGTTCGATGATTGATATCGTCATTTCTGCACACTCCACTATTGTTAATTTTTTTTTTTTTTTTTTTTTTGGGAACTGGCTTTCCAAGAAGAAGAGAAAGCTCGTAATAAGCAGGTGAGAAGGCAAAGTTCCGTGCATTTTTTTCGTTATTGACATGAATTTGTTTATTTTCCAGAGTTGATGAGCTCACTTTACATTGTTCGATTACAAGAAACCACAGATTTTGTCATGCTAAAATTGATTGAGAGAGAGAGAGAGAGGAATTATTTAAATCATTATTTGATAAATATGTAATGCAAAATTGTGGGAGCATTGGGACATCAAATAGAGCATGTTTTTCTCAGCTGTTGTGCTTTTCCATTCCATTTTACTTGGGAACATCTCGAGCTGAAAACCAACATGGCTATTGTAGATTGTACATGTATCAGGTATCTATGATGACACTTGATCCTTATTCTTCATACTTGGTCTAAAATGTTACTTTACGGTTATTTTGCAGATTCAAACTTCCATTGCTCAGACACTTCAACGACAAACTGAAGCTTTGGGTAGAAAACGTTCTAAACCTTCGAGGAGATTAAGTTCGAGAGGACGCAAAAGCTTCCAAAATCATATTGAGAGTCTATGCTTCGATGAAAATGAGGACGAAAATGATTATGATGTCAGCAAAAATTCATCTTCTGCTGATGAGCGCATGGACACCAGACCTAAAAGATCCAGGAGAGGTGGTCCTGTTAGATTTTCCCAGTCTTCTTCAGCTACTGGAGCAGATGGAGCAGATGGTGGTGGTATCGAAAATGATTATGAAGTGAACAAAGAAAAAGTTGGTGCATCTATAGGGTTTGTTGGTAGCTCAGAAAAACTTTCCTGGGGCAAAGGTGGAATAAGAAGTCACACTAGATATGGGGGTATAAATGGGGGGGCTGGTAAAAATTCCAGGAATAACCGCATCATGAAGCTGTCGGATTATGTTCGAAACTCAGAAAATGTCAGTGAGGAGGAGGTAAAAACAACAATTTGAGGTGTTACTAAACTTCCTAATGAATAAACTGTGGACATGTTAACGTATAAATGGCTCGCCTATTGAATCACATGCATGCTGTTATGCCTCTTTTAGCTGCTTAAAAGCTCAACGATCTTTTATGTTTCTATCCATCAATGACTTTTAACTTCTCTTCCTGACTTTTAGTTGGACATCCACCTTGTGCTCGTTTCCATGGATGAAACTAGAATACCCGCATTGCAAAGACCATATATTTGCTGCAGGCCTTCAGTGACGATTGGACACCTAAGTCAAGTAATCTGTCATTAAATGGAGCTGCACTTCATCCGTTTAAGGTCTTATTCATTTATTTAGTTGATTCTTGAGCTTCCTTTCTTCTGCAGTATGTCGCTCTCGAAACCGCGCTTCCCGTTGATGTTGTTGAAATGTGCATGGCGAAAGAGCTGCAAATGAAACTCAATCCTTCAACCTCCGGAACTCTAAAGAACCCATGTAAAGAAAGTATACAGATACTAAACGAGCAAGAAACGCTATCGACAGCCAAACTTAAAGCACACAGGCTAGCTTGTGGCTATCTGGTATGCCCTGAATCTACAGCACACAAATGCTTGAAAAAAATGCACCCACTACATCAATAA

mRNA sequence

CTTTGGTGTGTTTTTCTTTAATCAATCTAATGCCGTGGAAATTTCGTAAAACCACGAACACGAGGCTCCTTCCCTCTCGGCTCTGATATTTTGCTCCACCGCCCCCTTCATCATCTCTACGAACCTCAATTTTTGTGAACTTACAACTTCTTTTCGTTGTTTCTTCAACTTATTTTGCTTCAGACCGAAGCAATGCCGGCTCGGAAGCGCGCTTACGAGACTGTGAATGTTGATCTTTTGCCGCGACTCCATAATACCTTCAATGAATGCTCTGACGATGGAGAATCTGAGGAATCCGATCGAAGCCCTTCGTCCAGTCAAGGAGATAAGGACGAGTCTTGCTGCGACCGTATTCGAAGACCTGCTGTGTGGGCTTCACGTGCTGAAGCATTCGTCGATTTTTGTCCTGCACCGGAGTTTTCTTTACTTTCAAGTTTTGAGTTTCTTTACCGATTATGGTTAGTGGAATTGGGTGCTGGATCTTGTGGGCGAAGTGGGTTCTCGAGATTTATCGCCGTGAAATTGTCAGACATTCGGAGAGAAGTTCAATGTCCTATCTGCTTAGGAATCATCAGAAAAACCAGAACAGTGATGGAATGTCTGCACCGCTTCTGCAGGGAATGCATAGACAAATCGATGCGTCTAGGTAACAATGAGTGCCCTGCTTGCCGCACTCATTGTGCCAGTCGACGCTCTCTGAGAGATGATCCGAATTATGATACATTGATTGCTGTGTTATATCCAGATATTGACAAATATGAGGAAGAGATTCAAACTTCCATTGCTCAGACACTTCAACGACAAACTGAAGCTTTGGGTAGAAAACGTTCTAAACCTTCGAGGAGATTAAGTTCGAGAGGACGCAAAAGCTTCCAAAATCATATTGAGAGTCTATGCTTCGATGAAAATGAGGACGAAAATGATTATGATGTCAGCAAAAATTCATCTTCTGCTGATGAGCGCATGGACACCAGACCTAAAAGATCCAGGAGAGGTGGTCCTGTTAGATTTTCCCAGTCTTCTTCAGCTACTGGAGCAGATGGAGCAGATGGTGGTGGTATCGAAAATGATTATGAAGTGAACAAAGAAAAAGTTGGTGCATCTATAGGGTTTGTTGGTAGCTCAGAAAAACTTTCCTGGGGCAAAGGTGGAATAAGAAGTCACACTAGATATGGGGGTATAAATGGGGGGGCTGGTAAAAATTCCAGGAATAACCGCATCATGAAGCTGTCGGATTATGTTCGAAACTCAGAAAATGTCAGTGAGGAGGAGTTGGACATCCACCTTGTGCTCGTTTCCATGGATGAAACTAGAATACCCGCATTGCAAAGACCATATATTTGCTGCAGGCCTTCAGTGACGATTGGACACCTAAGTCAATATGTCGCTCTCGAAACCGCGCTTCCCGTTGATGTTGTTGAAATGTGCATGGCGAAAGAGCTGCAAATGAAACTCAATCCTTCAACCTCCGGAACTCTAAAGAACCCATGTAAAGAAAGTATACAGATACTAAACGAGCAAGAAACGCTATCGACAGCCAAACTTAAAGCACACAGGCTAGCTTGTGGCTATCTGGTATGCCCTGAATCTACAGCACACAAATGCTTGAAAAAAATGCACCCACTACATCAATAA

Coding sequence (CDS)

ATGCCGGCTCGGAAGCGCGCTTACGAGACTGTGAATGTTGATCTTTTGCCGCGACTCCATAATACCTTCAATGAATGCTCTGACGATGGAGAATCTGAGGAATCCGATCGAAGCCCTTCGTCCAGTCAAGGAGATAAGGACGAGTCTTGCTGCGACCGTATTCGAAGACCTGCTGTGTGGGCTTCACGTGCTGAAGCATTCGTCGATTTTTGTCCTGCACCGGAGTTTTCTTTACTTTCAAGTTTTGAGTTTCTTTACCGATTATGGTTAGTGGAATTGGGTGCTGGATCTTGTGGGCGAAGTGGGTTCTCGAGATTTATCGCCGTGAAATTGTCAGACATTCGGAGAGAAGTTCAATGTCCTATCTGCTTAGGAATCATCAGAAAAACCAGAACAGTGATGGAATGTCTGCACCGCTTCTGCAGGGAATGCATAGACAAATCGATGCGTCTAGGTAACAATGAGTGCCCTGCTTGCCGCACTCATTGTGCCAGTCGACGCTCTCTGAGAGATGATCCGAATTATGATACATTGATTGCTGTGTTATATCCAGATATTGACAAATATGAGGAAGAGATTCAAACTTCCATTGCTCAGACACTTCAACGACAAACTGAAGCTTTGGGTAGAAAACGTTCTAAACCTTCGAGGAGATTAAGTTCGAGAGGACGCAAAAGCTTCCAAAATCATATTGAGAGTCTATGCTTCGATGAAAATGAGGACGAAAATGATTATGATGTCAGCAAAAATTCATCTTCTGCTGATGAGCGCATGGACACCAGACCTAAAAGATCCAGGAGAGGTGGTCCTGTTAGATTTTCCCAGTCTTCTTCAGCTACTGGAGCAGATGGAGCAGATGGTGGTGGTATCGAAAATGATTATGAAGTGAACAAAGAAAAAGTTGGTGCATCTATAGGGTTTGTTGGTAGCTCAGAAAAACTTTCCTGGGGCAAAGGTGGAATAAGAAGTCACACTAGATATGGGGGTATAAATGGGGGGGCTGGTAAAAATTCCAGGAATAACCGCATCATGAAGCTGTCGGATTATGTTCGAAACTCAGAAAATGTCAGTGAGGAGGAGTTGGACATCCACCTTGTGCTCGTTTCCATGGATGAAACTAGAATACCCGCATTGCAAAGACCATATATTTGCTGCAGGCCTTCAGTGACGATTGGACACCTAAGTCAATATGTCGCTCTCGAAACCGCGCTTCCCGTTGATGTTGTTGAAATGTGCATGGCGAAAGAGCTGCAAATGAAACTCAATCCTTCAACCTCCGGAACTCTAAAGAACCCATGTAAAGAAAGTATACAGATACTAAACGAGCAAGAAACGCTATCGACAGCCAAACTTAAAGCACACAGGCTAGCTTGTGGCTATCTGGTATGCCCTGAATCTACAGCACACAAATGCTTGAAAAAAATGCACCCACTACATCAATAA

Protein sequence

MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVWASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEEIQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLVCPESTAHKCLKKMHPLHQ

Homology

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BLAST of Lsi01G003010.1 vs. ExPASy Swiss-Prot
Match: Q9FKW0 (Putative E3 ubiquitin-protein ligase RING1a OS=Arabidopsis thaliana OX=3702 GN=RING1A PE=1 SV=2)

HSP 1 Score: 283.9 bits (725), Expect = 3.4e-75
Identity = 171/383 (44.65%), Postives = 237/383 (61.88%), Query Frame = 0


Query: 104 SRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHC 163
           S F+ + L +IR++VQCPICLGII+KTRTVMECLHRFCRECIDKSMRLGNNECPACR HC
Sbjct: 120 SEFMEIDLGEIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRKHC 179

Query: 164 ASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQTLQRQTEALG 223
           ASRRSLRDDP +D LIA L+ +ID YEEE              IQ SIAQ  QRQ+EAL 
Sbjct: 180 ASRRSLRDDPKFDALIAALFTNIDSYEEEELAFHEDEMARNKQIQASIAQISQRQSEALV 239

Query: 224 RKRS---------------KPSRRLSSRGRKSFQNHIESLCFDENEDENDYDVSKNSSSA 283
           ++RS                 SRR  +      QN  E+   D+N+D N+    K+SSS 
Sbjct: 240 KRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQQNASEAHEDDDNDDNNNRGRDKDSSSD 299

Query: 284 DERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGSSEKL 343
           +   + R K+ R+    R +Q  S++GA+  +G   +ND EV ++  G S G V + E L
Sbjct: 300 ERGTEVRQKKRRKRSTSRSTQHPSSSGANKNNGNCADNDTEVYRDTKGISPGLVWNPEIL 359

Query: 344 SWGKGGIRSHTRYG-GINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSMDET 403
           +WG+GG RS+TR+G   +GG+ K+ RN R+ KL +Y+R+S + S  ELDIH+ LVS+D  
Sbjct: 360 AWGRGGTRSNTRHGNNTSGGSSKSVRNARVNKLVEYLRSSVDGSSVELDIHVKLVSLDTK 419

Query: 404 RIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAK-----ELQMKLNPSTSGT 452
            IP L +PY+CCRP++ +  L ++VAL+  L  + VE+ + +     +  ++  P+ +  
Sbjct: 420 CIPDLPQPYLCCRPTLLVKQLREFVALQIHLKTEEVELLVTRRRVGEDAAIENLPAVTPA 479

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BLAST of Lsi01G003010.1 vs. ExPASy Swiss-Prot
Match: Q0WX00 (Putative E3 ubiquitin-protein ligase RING1b OS=Arabidopsis thaliana OX=3702 GN=RING1B PE=1 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.8e-63
Identity = 168/393 (42.75%), Postives = 230/393 (58.52%), Query Frame = 0


Query: 95  AGSCGRSGFSRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 154
           + S G    S ++ V L+DI ++VQC ICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN
Sbjct: 78  SSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 137

Query: 155 ECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQT 214
           ECP CR HCASRRSLRDDPN+D LIA L+ +IDK+EEE              IQ SIAQ 
Sbjct: 138 ECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQIQASIAQV 197

Query: 215 LQRQTEALGRKRS--------KPSRRL--SSRGRKSFQNHIESLCFDENEDENDYDVSKN 274
            QRQ++AL +++S          SRR    SR R++ +N IE    + N+D++     K+
Sbjct: 198 SQRQSKALVKRKSVGKGTAILSRSRRSGGGSRRRRNCRN-IEQDTSEANDDDDQNKRGKD 257

Query: 275 SSSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGS 334
           SSS +     R KRS          SS+A   D   G G E  ++ +   +   +  V +
Sbjct: 258 SSSDEPCERQRKKRS-----ATQPSSSNANNNDNCAGNGTEQTHQRDSRVISPVL--VWN 317

Query: 335 SEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSM 394
           SE ++WG+GG RS+TR G  N GA  + RN R+ +L +Y+ + E  +  ELDIHL LVS+
Sbjct: 318 SELIAWGRGGTRSNTRQGNNNQGA-ISKRNARLKRLVEYLGSLEG-NSVELDIHLKLVSL 377

Query: 395 DETRIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAKELQMKLNPSTSGTLK 454
           D   +  L  PY+C RP++ +  L +YVA    L  + VE+ ++K+    +   TS    
Sbjct: 378 DTEGLLNLHEPYLCFRPTLLVKQLREYVARHLKLKAEEVELLVSKDGDTVIGNKTS---- 437

Query: 455 NPCKESIQILNEQETLSTAKLKAHRLAC-GYLV 463
               E +Q L + ET+  AKLK   ++  GY++
Sbjct: 438 ---TEKMQSLQDDETV--AKLKVDCISSNGYMI 451

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BLAST of Lsi01G003010.1 vs. ExPASy Swiss-Prot
Match: Q5TJF3 (E3 ubiquitin-protein ligase RING1 OS=Canis lupus familiaris OX=9615 GN=RING1 PE=3 SV=2)

HSP 1 Score: 99.8 bits (247), Expect = 9.2e-20
Identity = 66/197 (33.50%), Postives = 94/197 (47.72%), Query Frame = 0


Query: 107 IAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASR 166
           IAV    +  E+ CPICL +++ T T  ECLHRFC +CI  ++R GN ECP CR    S+
Sbjct: 35  IAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSK 94

Query: 167 RSLRDDPNYDTLIAVLYPDIDKYEEE-----IQTSIAQTLQRQTEALGRKRSKPSRRLSS 226
           RSLR DPN+D LI+ +YP  ++YE       I+ S     Q  + ++       +   + 
Sbjct: 95  RSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQ 154

Query: 227 RGRKSFQNHIESLCF-----DENEDENDYDVSKNSSSADERMDTRPKRSRRGGPVRFSQS 286
           R R+      ++        +  E E D +   + S+ D      PKR R GG    S  
Sbjct: 155 RVRRPMPGSDQTTTMSGGEGEPGEGEGDGEDVSSDSAPDSAPGPAPKRPRGGGAGGSSVG 214

Query: 287 SSATGADGADGGGIEND 294
           +   GA G  GG    D
Sbjct: 215 TGGGGAGGVGGGAGSED 231

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BLAST of Lsi01G003010.1 vs. ExPASy Swiss-Prot
Match: A1YER5 (E3 ubiquitin-protein ligase RING1 OS=Gorilla gorilla gorilla OX=9595 GN=RING1 PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 9.2e-20
Identity = 66/197 (33.50%), Postives = 93/197 (47.21%), Query Frame = 0


Query: 107 IAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASR 166
           IAV    +  E+ CPICL +++ T T  ECLHRFC +CI  ++R GN ECP CR    S+
Sbjct: 6   IAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSK 65

Query: 167 RSLRDDPNYDTLIAVLYPDIDKYEEE-----IQTSIAQTLQRQTEALGRKRSKPSRRLSS 226
           RSLR DPN+D LI+ +YP  ++YE       I+ S     Q  + ++       +   + 
Sbjct: 66  RSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQ 125

Query: 227 RGRKSFQNHIESLCF-----DENEDENDYDVSKNSSSADERMDTRPKRSRRGGPVRFSQS 286
           R R+      ++        D  E E D +   + S+ D      PKR R GG    S  
Sbjct: 126 RVRRPIPGSDQTTTMSGGEGDPGEGEGDGEDVSSDSAPDSAPGPAPKRPRGGGAGGSSVG 185

Query: 287 SSATGADGADGGGIEND 294
           +   G  G  GG    D
Sbjct: 186 TGGGGTGGVGGGAGSED 202

[Close]

BLAST of Lsi01G003010.1 vs. ExPASy Swiss-Prot
Match: Q06587 (E3 ubiquitin-protein ligase RING1 OS=Homo sapiens OX=9606 GN=RING1 PE=1 SV=2)

HSP 1 Score: 98.2 bits (243), Expect = 2.7e-19
Identity = 65/197 (32.99%), Postives = 93/197 (47.21%), Query Frame = 0


Query: 107 IAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASR 166
           IAV    +  E+ CPICL +++ T T  ECLHRFC +CI  ++R GN ECP CR    S+
Sbjct: 35  IAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSK 94

Query: 167 RSLRDDPNYDTLIAVLYPDIDKYEEE-----IQTSIAQTLQRQTEALGRKRSKPSRRLSS 226
           RSLR DPN+D LI+ +YP  ++YE       I+ S     Q  + ++       +   + 
Sbjct: 95  RSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQ 154

Query: 227 RGRKSFQNHIESLCF-----DENEDENDYDVSKNSSSADERMDTRPKRSRRGGPVRFSQS 286
           R R+      ++        +  E E D +   + S+ D      PKR R GG    S  
Sbjct: 155 RVRRPIPGSDQTTTMSGGEGEPGEGEGDGEDVSSDSAPDSAPGPAPKRPRGGGAGGSSVG 214

Query: 287 SSATGADGADGGGIEND 294
           +   G  G  GG    D
Sbjct: 215 TGGGGTGGVGGGAGSED 231

[Close]

BLAST of Lsi01G003010.1 vs. ExPASy TrEMBL
Match: A0A6J1EFU8 (putative E3 ubiquitin-protein ligase RING1a isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433005 PE=4 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 2.5e-190
Identity = 366/493 (74.24%), Postives = 388/493 (78.70%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVW 60
           MPARKR YETV+ DLLPRLH+TFN+ S+DGESEESDRS SSSQ DKDE            
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSSQEDKDE------------ 60

Query: 61  ASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQC 120
                                                        F+AVKLSDIRR+VQC
Sbjct: 61  ---------------------------------------------FVAVKLSDIRRDVQC 120

Query: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIA 180
           PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIA
Sbjct: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIA 180

Query: 181 VLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKS 240
           VLYPDIDKYEEE              IQ+SIAQTL+RQTEALGRKRSKP RRLSSRGRKS
Sbjct: 181 VLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGRKS 240

Query: 241 FQNHIESLCFDENEDENDYDVSKNSSSADERMDTRP-KRSRRGGPVRFSQSSSATGADGA 300
           FQNHIESL FDENEDENDYD SKNSSSADERMDTRP KRSRRGGPVRFSQSSSATGADGA
Sbjct: 241 FQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGADGA 300

Query: 301 DGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMK 360
           DGG  ENDYEVNKE+VGAS+G V SSEKLSWGKGGIRSHTRYGGINGG GK SRNNRI K
Sbjct: 301 DGGSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNNRITK 360

Query: 361 LSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETALP 420
           LSDYVRNSEN+SEEELD+ L+LVSMD+TRIPAL+  YICCRPSVTIGHL Q VALETAL 
Sbjct: 361 LSDYVRNSENISEEELDVQLMLVSMDKTRIPALRSSYICCRPSVTIGHLRQLVALETALS 420

Query: 421 VDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLVCPE 479
           VDV+EM + KELQMK+NPSTSGT  +PCKES QILN+QETLS  KLK HRLACGYLVC E
Sbjct: 421 VDVIEMYVVKELQMKINPSTSGTFLDPCKESAQILNQQETLSMDKLKTHRLACGYLVCLE 436

[Close]

BLAST of Lsi01G003010.1 vs. ExPASy TrEMBL
Match: A0A5D3CNZ3 (Putative E3 ubiquitin-protein ligase RING1a OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007610 PE=4 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 2.4e-188
Identity = 377/511 (73.78%), Postives = 405/511 (79.26%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLL-PRLHNTF-NECSDDGESEESDRSPSSSQGDKDE--SCCDRIRR 60
           MPARKRAY++++VDLL PRLHNTF NE S D +S+ESDRS SSSQGD+DE       +  
Sbjct: 1   MPARKRAYDSLDVDLLPPRLHNTFNNERSQDEQSQESDRSSSSSQGDEDEYVPFMSSVLS 60

Query: 61  PAVWASRAEAFVDFCPAPEFSLLSSFEF--------------LYRLWLVELGAGSCGRSG 120
             ++       V F     F L     +              L+ +  VELGAGSCGRS 
Sbjct: 61  LLLFVRLLFRVVPFYSIYSFDLFRLVGYGVSFVANCLGLIWDLHGVPRVELGAGSCGRSE 120

Query: 121 FSR-----------------FIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECI 180
           FSR                 FIAVKLSDIR+EVQCPICLGII+KTRTVMECLHRFCRECI
Sbjct: 121 FSRLYVRSICFYICYELLGGFIAVKLSDIRKEVQCPICLGIIKKTRTVMECLHRFCRECI 180

Query: 181 DKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE------------- 240
           DKSMRLGN ECPACRTHCASRRSLRDDPNYDTLIAVLYPDI+KYEEE             
Sbjct: 181 DKSMRLGNKECPACRTHCASRRSLRDDPNYDTLIAVLYPDIEKYEEEELAFQEEEKARNK 240

Query: 241 -IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKSFQNHIESLCFDENEDENDYDVSKNS 300
            IQTSIAQTLQRQTE+LGRKRSKPSRRLSSRG KSFQNHIESLCFDENEDENDYDVSKNS
Sbjct: 241 QIQTSIAQTLQRQTESLGRKRSKPSRRLSSRGPKSFQNHIESLCFDENEDENDYDVSKNS 300

Query: 301 SSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGSS 360
           SSADERMDTRPKR RRGGPVRFSQSSSATGADGAD GGIE+DY      VGAS+G VGSS
Sbjct: 301 SSADERMDTRPKRPRRGGPVRFSQSSSATGADGADVGGIEHDY------VGASLGLVGSS 360

Query: 361 EKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSMD 420
           EKLSWGKGGIRSHTRYGG NGGAGKNSRNNRI KLSDYVRNSENVSEEELDIH++LVSMD
Sbjct: 361 EKLSWGKGGIRSHTRYGGTNGGAGKNSRNNRIAKLSDYVRNSENVSEEELDIHVLLVSMD 420

Query: 421 ETRIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAKELQMKLNPSTSGTLKN 463
            + IPALQRPYICCRPSV IGHLSQYVALETAL VDVVE+C+A ELQMKL+PSTS  +KN
Sbjct: 421 RS-IPALQRPYICCRPSVMIGHLSQYVALETALSVDVVEICVANELQMKLDPSTSEAIKN 480

[Close]

BLAST of Lsi01G003010.1 vs. ExPASy TrEMBL
Match: A0A0A0KLI2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517990 PE=4 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 8.5e-186
Identity = 363/478 (75.94%), Postives = 386/478 (80.75%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLL-PRLHNTF-NECSDDGESEESDRSPSSSQGDKDESCCDRIRRPA 60
           MPARKRAY++++VDLL PRLHNTF NE S D +S+ESDRS SSSQGD+DE          
Sbjct: 1   MPARKRAYDSLDVDLLPPRLHNTFNNERSQDEQSQESDRSSSSSQGDEDE---------- 60

Query: 61  VWASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREV 120
                                                          FIAVKLSDIR+EV
Sbjct: 61  -----------------------------------------------FIAVKLSDIRKEV 120

Query: 121 QCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTL 180
           QCPICLGII+KTRTVMECLHRFCRECIDKSMRLGN ECPACRTHCASRRSLRDDPNYDTL
Sbjct: 121 QCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNYDTL 180

Query: 181 IAVLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGR 240
           IAVLYPDI+KYEEE              IQTSIAQTLQRQTE+LGRKRSKPSRRLSSRG 
Sbjct: 181 IAVLYPDIEKYEEEELAFQEEEKARNKQIQTSIAQTLQRQTESLGRKRSKPSRRLSSRGP 240

Query: 241 KSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKRSRRGGPVRFSQSSSATGADG 300
           KSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKR RR GPVRFSQSSSATGADG
Sbjct: 241 KSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKRPRRCGPVRFSQSSSATGADG 300

Query: 301 ADGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIM 360
           ADGGGIE++YEVNKEKVGAS+G VGSSEKLSWGKGGIRSHTRYGG NGGAGK SRNNRI 
Sbjct: 301 ADGGGIEHEYEVNKEKVGASLGLVGSSEKLSWGKGGIRSHTRYGGTNGGAGKISRNNRIA 360

Query: 361 KLSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETAL 420
           KLSDYVRNSENVSEEELDIH++LVSMD T IPALQRPYICCRPSV IGHLSQYVALET+L
Sbjct: 361 KLSDYVRNSENVSEEELDIHVLLVSMDRT-IPALQRPYICCRPSVMIGHLSQYVALETSL 420

Query: 421 PVDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLV 463
            VDVVE+C+AKELQ+KL+PSTS   KNPCKES+QILNEQETLSTAKLKAHRLACGYL+
Sbjct: 421 SVDVVEICVAKELQVKLDPSTSEATKNPCKESVQILNEQETLSTAKLKAHRLACGYLL 420

[Close]

BLAST of Lsi01G003010.1 vs. ExPASy TrEMBL
Match: A0A6J1IQZ1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478584 PE=4 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 7.2e-185
Identity = 362/494 (73.28%), Postives = 385/494 (77.94%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVW 60
           MPARKR YETV+ DLLPRLH+TFN+ S+DGESEESDRS SSSQ D+DE            
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSSQEDEDE------------ 60

Query: 61  ASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQC 120
                                                        F+AVKLSDIRR+VQC
Sbjct: 61  ---------------------------------------------FVAVKLSDIRRDVQC 120

Query: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIA 180
           PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIA
Sbjct: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIA 180

Query: 181 VLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKS 240
           VLYPDIDKYEEE              IQ+SIAQTL+RQTEALGRKRSKP RRLSSRG+KS
Sbjct: 181 VLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGQKS 240

Query: 241 FQNHIESLCFDENEDENDYDVSKNSSSADERMDTRP-KRSRRGGPVRFSQSSSATGADGA 300
           FQNHIESL FDENEDENDYD SKNSSSADERMDTRP KRSRRGGPVRFSQSSSAT   GA
Sbjct: 241 FQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSAT---GA 300

Query: 301 DGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMK 360
           DGGG END EVNKEKVGAS+G V SSEK SWGKGGIRSHTRYGGINGG GK SRNNRI K
Sbjct: 301 DGGGNENDCEVNKEKVGASMGLVSSSEKHSWGKGGIRSHTRYGGINGGGGKISRNNRITK 360

Query: 361 LSDYVRNSENVS-EEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETAL 420
           LSDYVRNSEN+S EEELD+HL+LVSMD+TRIPAL+ PYICCRPSVTIGHL Q VAL TAL
Sbjct: 361 LSDYVRNSENISEEEELDVHLMLVSMDKTRIPALRSPYICCRPSVTIGHLRQLVALVTAL 420

Query: 421 PVDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLVCP 479
            VDV+EM + KELQMK+NPSTSG   +PCKES QILN+QETLS  KLK HRLACGYLVC 
Sbjct: 421 SVDVIEMYVVKELQMKINPSTSGAFLDPCKESAQILNQQETLSMDKLKTHRLACGYLVCL 434

[Close]

BLAST of Lsi01G003010.1 vs. ExPASy TrEMBL
Match: A0A6J1ECT6 (putative E3 ubiquitin-protein ligase RING1a isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433005 PE=4 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 1.5e-182
Identity = 354/477 (74.21%), Postives = 376/477 (78.83%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVW 60
           MPARKR YETV+ DLLPRLH+TFN+ S+DGESEESDRS SSSQ DKDE            
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSSQEDKDE------------ 60

Query: 61  ASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQC 120
                                                        F+AVKLSDIRR+VQC
Sbjct: 61  ---------------------------------------------FVAVKLSDIRRDVQC 120

Query: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIA 180
           PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIA
Sbjct: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIA 180

Query: 181 VLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKS 240
           VLYPDIDKYEEE              IQ+SIAQTL+RQTEALGRKRSKP RRLSSRGRKS
Sbjct: 181 VLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGRKS 240

Query: 241 FQNHIESLCFDENEDENDYDVSKNSSSADERMDTRP-KRSRRGGPVRFSQSSSATGADGA 300
           FQNHIESL FDENEDENDYD SKNSSSADERMDTRP KRSRRGGPVRFSQSSSATGADGA
Sbjct: 241 FQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGADGA 300

Query: 301 DGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMK 360
           DGG  ENDYEVNKE+VGAS+G V SSEKLSWGKGGIRSHTRYGGINGG GK SRNNRI K
Sbjct: 301 DGGSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNNRITK 360

Query: 361 LSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETALP 420
           LSDYVRNSEN+SEEELD+ L+LVSMD+TRIPAL+  YICCRPSVTIGHL Q VALETAL 
Sbjct: 361 LSDYVRNSENISEEELDVQLMLVSMDKTRIPALRSSYICCRPSVTIGHLRQLVALETALS 420

Query: 421 VDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLV 463
           VDV+EM + KELQMK+NPSTSGT  +PCKES QILN+QETLS  KLK HRLACGYL+
Sbjct: 421 VDVIEMYVVKELQMKINPSTSGTFLDPCKESAQILNQQETLSMDKLKTHRLACGYLL 420

[Close]

BLAST of Lsi01G003010.1 vs. NCBI nr
Match: XP_038881714.1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 [Benincasa hispida])

HSP 1 Score: 699.5 bits (1804), Expect = 2.0e-197
Identity = 376/476 (78.99%), Postives = 392/476 (82.35%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVW 60
           MPARKRAY+TV+VDLLPRLHNTFNECS+DG+SEESDRSPSSSQGDKDE            
Sbjct: 1   MPARKRAYDTVDVDLLPRLHNTFNECSEDGQSEESDRSPSSSQGDKDE------------ 60

Query: 61  ASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQC 120
                                                        FIAVKLSDIR+EVQC
Sbjct: 61  ---------------------------------------------FIAVKLSDIRKEVQC 120

Query: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIA 180
           PICLGII+KTRTVMECLHRFCRECIDKSMRLGN ECPACRTHCASRRSLRDDPNYDTLIA
Sbjct: 121 PICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNYDTLIA 180

Query: 181 VLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKS 240
           VLYPDIDKYEEE              IQTSIAQTLQRQTEALGRKRSKPSRRLSSRG KS
Sbjct: 181 VLYPDIDKYEEEELAFQEEEKGRNKQIQTSIAQTLQRQTEALGRKRSKPSRRLSSRGSKS 240

Query: 241 FQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKRSRRGGPVRFSQSSSATGADGAD 300
           FQNHIES+CFDENEDENDYDVSKNSSSADERMD RPKRSRRGGPVRFSQSSSATGADG D
Sbjct: 241 FQNHIESICFDENEDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATGADGTD 300

Query: 301 GGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKL 360
            GGIENDYEV KEKVGASIG VGSSEKLSWGKGGIRSHTRYGG+NGGAGKN RNNRIMKL
Sbjct: 301 -GGIENDYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNNRIMKL 360

Query: 361 SDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETALPV 420
           SDYVRNSENVSE+ELDIHLVL+S+DETR+PALQRPYI CRPSVTIGHLSQYVAL+T L V
Sbjct: 361 SDYVRNSENVSEKELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALKTGLSV 418

Query: 421 DVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLV 463
           DVVEMC+AKELQMKLNPS SGTLKNPCKE IQILNEQETLSTAK+KAHRLACGYL+
Sbjct: 421 DVVEMCVAKELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYLL 418

[Close]

BLAST of Lsi01G003010.1 vs. NCBI nr
Match: XP_023543673.1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 679.5 bits (1752), Expect = 2.1e-191
Identity = 367/493 (74.44%), Postives = 389/493 (78.90%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVW 60
           MPARKR YETV+ DLLPRLH+TFN+ S+DGESEESDRS SS Q DKDE            
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSGQEDKDE------------ 60

Query: 61  ASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQC 120
                                                        F+AVKLSDIRR+VQC
Sbjct: 61  ---------------------------------------------FVAVKLSDIRRDVQC 120

Query: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIA 180
           PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIA
Sbjct: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIA 180

Query: 181 VLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKS 240
           VLYPDIDKYEEE              IQ+SIAQTL+RQTEALGRKRSKP RRLSSRGRKS
Sbjct: 181 VLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGRKS 240

Query: 241 FQNHIESLCFDENEDENDYDVSKNSSSADERMDTRP-KRSRRGGPVRFSQSSSATGADGA 300
           FQNHIESL FDENEDENDYD SKNSSSADERMDTRP KRSRRGGPVRFSQSSSATGADGA
Sbjct: 241 FQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGADGA 300

Query: 301 DGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMK 360
           DGG  ENDYEVNKE+VGAS+G V SSEKLSWGKGGIRSHTRYGGINGG GK SRNNRI K
Sbjct: 301 DGGSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNNRITK 360

Query: 361 LSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETALP 420
           LSDYVRNSEN+SEEELD+HL+LVSMD+TRIPAL+ PYICCRPSVTIGHL Q VALETAL 
Sbjct: 361 LSDYVRNSENISEEELDVHLMLVSMDKTRIPALRSPYICCRPSVTIGHLRQLVALETALS 420

Query: 421 VDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLVCPE 479
           VDV+EM + KELQMK+NPSTSGT  +PCKES QILN+QETLS  KLK HRLACGYLVC E
Sbjct: 421 VDVIEMYVVKELQMKINPSTSGTFLDPCKESAQILNQQETLSMDKLKTHRLACGYLVCLE 436

[Close]

BLAST of Lsi01G003010.1 vs. NCBI nr
Match: XP_022925653.1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 [Cucurbita moschata])

HSP 1 Score: 674.9 bits (1740), Expect = 5.3e-190
Identity = 366/493 (74.24%), Postives = 388/493 (78.70%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDESCCDRIRRPAVW 60
           MPARKR YETV+ DLLPRLH+TFN+ S+DGESEESDRS SSSQ DKDE            
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSSQEDKDE------------ 60

Query: 61  ASRAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRREVQC 120
                                                        F+AVKLSDIRR+VQC
Sbjct: 61  ---------------------------------------------FVAVKLSDIRRDVQC 120

Query: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIA 180
           PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIA
Sbjct: 121 PICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIA 180

Query: 181 VLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKS 240
           VLYPDIDKYEEE              IQ+SIAQTL+RQTEALGRKRSKP RRLSSRGRKS
Sbjct: 181 VLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGRKS 240

Query: 241 FQNHIESLCFDENEDENDYDVSKNSSSADERMDTRP-KRSRRGGPVRFSQSSSATGADGA 300
           FQNHIESL FDENEDENDYD SKNSSSADERMDTRP KRSRRGGPVRFSQSSSATGADGA
Sbjct: 241 FQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGADGA 300

Query: 301 DGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMK 360
           DGG  ENDYEVNKE+VGAS+G V SSEKLSWGKGGIRSHTRYGGINGG GK SRNNRI K
Sbjct: 301 DGGSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNNRITK 360

Query: 361 LSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALETALP 420
           LSDYVRNSEN+SEEELD+ L+LVSMD+TRIPAL+  YICCRPSVTIGHL Q VALETAL 
Sbjct: 361 LSDYVRNSENISEEELDVQLMLVSMDKTRIPALRSSYICCRPSVTIGHLRQLVALETALS 420

Query: 421 VDVVEMCMAKELQMKLNPSTSGTLKNPCKESIQILNEQETLSTAKLKAHRLACGYLVCPE 479
           VDV+EM + KELQMK+NPSTSGT  +PCKES QILN+QETLS  KLK HRLACGYLVC E
Sbjct: 421 VDVIEMYVVKELQMKINPSTSGTFLDPCKESAQILNQQETLSMDKLKTHRLACGYLVCLE 436

[Close]

BLAST of Lsi01G003010.1 vs. NCBI nr
Match: KAG7034603.1 (putative E3 ubiquitin-protein ligase RING1a, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 671.4 bits (1731), Expect = 5.8e-189
Identity = 379/541 (70.06%), Postives = 404/541 (74.68%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLLPRLHNTFNECSDDGESEESDRSPSSSQGDKDE------------ 60
           MPARKR YETV+ DLLPRLH+TFN+ S+DGESEESDRS SSSQ DKDE            
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSSQEDKDEYVPFMMSSVFGL 60

Query: 61  ------------------------------------SC------------CDRIRRPAVW 120
                                                C               + RP+  
Sbjct: 61  LLFVRHLLRAVPFYSIFSFDLFHLVGYGVLWCLTAWDCFGICMVCFVVLFSKFLLRPSSK 120

Query: 121 AS----RAEAFVDFCPAPEFSLLSSFEFLYRLWLVELGAGSCGRSGFSRFIAVKLSDIRR 180
            S     +EAFV F PAPE              LVELGAGSC     SRF+AVKLSDIRR
Sbjct: 121 TSCVVFTSEAFVGFYPAPE--------------LVELGAGSC---WLSRFVAVKLSDIRR 180

Query: 181 EVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYD 240
           +VQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+D
Sbjct: 181 DVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFD 240

Query: 241 TLIAVLYPDIDKYEEE--------------IQTSIAQTLQRQTEALGRKRSKPSRRLSSR 300
           TLIAVLYPDIDKYEEE              IQ+SIAQTL+RQTEALGRKRSKP RRLSSR
Sbjct: 241 TLIAVLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSR 300

Query: 301 GRKSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRP-KRSRRGGPVRFSQSSSATG 360
           GRKSFQNHIESL FDENEDENDYD SKNSSSADERMDTRP KRSRRGGP+RFSQSSSATG
Sbjct: 301 GRKSFQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPIRFSQSSSATG 360

Query: 361 ADGADGGGIENDYEVNKEKVGASIGFVGSSEKLSWGKGGIRSHTRYGGINGGAGKNSRNN 420
           ADGADGG  ENDYEVNKE+VGAS+G V SSEKLSWGKGGIRSHTRYGGINGG GK SRNN
Sbjct: 361 ADGADGGSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNN 420

Query: 421 RIMKLSDYVRNSENVSEEELDIHLVLVSMDETRIPALQRPYICCRPSVTIGHLSQYVALE 463
           RI KLSDYVRNSEN+SEEELD+ L+LVSMD+TRIPAL+ PYICCRPSVTIGHL Q VALE
Sbjct: 421 RITKLSDYVRNSENISEEELDVQLMLVSMDKTRIPALRSPYICCRPSVTIGHLRQLVALE 480

[Close]

BLAST of Lsi01G003010.1 vs. NCBI nr
Match: TYK13150.1 (putative E3 ubiquitin-protein ligase RING1a [Cucumis melo var. makuwa])

HSP 1 Score: 668.3 bits (1723), Expect = 4.9e-188
Identity = 377/511 (73.78%), Postives = 405/511 (79.26%), Query Frame = 0


Query: 1   MPARKRAYETVNVDLL-PRLHNTF-NECSDDGESEESDRSPSSSQGDKDE--SCCDRIRR 60
           MPARKRAY++++VDLL PRLHNTF NE S D +S+ESDRS SSSQGD+DE       +  
Sbjct: 1   MPARKRAYDSLDVDLLPPRLHNTFNNERSQDEQSQESDRSSSSSQGDEDEYVPFMSSVLS 60

Query: 61  PAVWASRAEAFVDFCPAPEFSLLSSFEF--------------LYRLWLVELGAGSCGRSG 120
             ++       V F     F L     +              L+ +  VELGAGSCGRS 
Sbjct: 61  LLLFVRLLFRVVPFYSIYSFDLFRLVGYGVSFVANCLGLIWDLHGVPRVELGAGSCGRSE 120

Query: 121 FSR-----------------FIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECI 180
           FSR                 FIAVKLSDIR+EVQCPICLGII+KTRTVMECLHRFCRECI
Sbjct: 121 FSRLYVRSICFYICYELLGGFIAVKLSDIRKEVQCPICLGIIKKTRTVMECLHRFCRECI 180

Query: 181 DKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE------------- 240
           DKSMRLGN ECPACRTHCASRRSLRDDPNYDTLIAVLYPDI+KYEEE             
Sbjct: 181 DKSMRLGNKECPACRTHCASRRSLRDDPNYDTLIAVLYPDIEKYEEEELAFQEEEKARNK 240

Query: 241 -IQTSIAQTLQRQTEALGRKRSKPSRRLSSRGRKSFQNHIESLCFDENEDENDYDVSKNS 300
            IQTSIAQTLQRQTE+LGRKRSKPSRRLSSRG KSFQNHIESLCFDENEDENDYDVSKNS
Sbjct: 241 QIQTSIAQTLQRQTESLGRKRSKPSRRLSSRGPKSFQNHIESLCFDENEDENDYDVSKNS 300

Query: 301 SSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGSS 360
           SSADERMDTRPKR RRGGPVRFSQSSSATGADGAD GGIE+DY      VGAS+G VGSS
Sbjct: 301 SSADERMDTRPKRPRRGGPVRFSQSSSATGADGADVGGIEHDY------VGASLGLVGSS 360

Query: 361 EKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSMD 420
           EKLSWGKGGIRSHTRYGG NGGAGKNSRNNRI KLSDYVRNSENVSEEELDIH++LVSMD
Sbjct: 361 EKLSWGKGGIRSHTRYGGTNGGAGKNSRNNRIAKLSDYVRNSENVSEEELDIHVLLVSMD 420

Query: 421 ETRIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAKELQMKLNPSTSGTLKN 463
            + IPALQRPYICCRPSV IGHLSQYVALETAL VDVVE+C+A ELQMKL+PSTS  +KN
Sbjct: 421 RS-IPALQRPYICCRPSVMIGHLSQYVALETALSVDVVEICVANELQMKLDPSTSEAIKN 480

[Close]

BLAST of Lsi01G003010.1 vs. TAIR 10
Match: AT5G44280.1 (RING 1A )

HSP 1 Score: 283.9 bits (725), Expect = 2.4e-76
Identity = 171/383 (44.65%), Postives = 237/383 (61.88%), Query Frame = 0


Query: 104 SRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHC 163
           S F+ + L +IR++VQCPICLGII+KTRTVMECLHRFCRECIDKSMRLGNNECPACR HC
Sbjct: 120 SEFMEIDLGEIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRKHC 179

Query: 164 ASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQTLQRQTEALG 223
           ASRRSLRDDP +D LIA L+ +ID YEEE              IQ SIAQ  QRQ+EAL 
Sbjct: 180 ASRRSLRDDPKFDALIAALFTNIDSYEEEELAFHEDEMARNKQIQASIAQISQRQSEALV 239

Query: 224 RKRS---------------KPSRRLSSRGRKSFQNHIESLCFDENEDENDYDVSKNSSSA 283
           ++RS                 SRR  +      QN  E+   D+N+D N+    K+SSS 
Sbjct: 240 KRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQQNASEAHEDDDNDDNNNRGRDKDSSSD 299

Query: 284 DERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGSSEKL 343
           +   + R K+ R+    R +Q  S++GA+  +G   +ND EV ++  G S G V + E L
Sbjct: 300 ERGTEVRQKKRRKRSTSRSTQHPSSSGANKNNGNCADNDTEVYRDTKGISPGLVWNPEIL 359

Query: 344 SWGKGGIRSHTRYG-GINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSMDET 403
           +WG+GG RS+TR+G   +GG+ K+ RN R+ KL +Y+R+S + S  ELDIH+ LVS+D  
Sbjct: 360 AWGRGGTRSNTRHGNNTSGGSSKSVRNARVNKLVEYLRSSVDGSSVELDIHVKLVSLDTK 419

Query: 404 RIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAK-----ELQMKLNPSTSGT 452
            IP L +PY+CCRP++ +  L ++VAL+  L  + VE+ + +     +  ++  P+ +  
Sbjct: 420 CIPDLPQPYLCCRPTLLVKQLREFVALQIHLKTEEVELLVTRRRVGEDAAIENLPAVTPA 479

[Close]

BLAST of Lsi01G003010.1 vs. TAIR 10
Match: AT1G03770.1 (RING 1B )

HSP 1 Score: 245.0 bits (624), Expect = 1.3e-64
Identity = 168/393 (42.75%), Postives = 230/393 (58.52%), Query Frame = 0


Query: 95  AGSCGRSGFSRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 154
           + S G    S ++ V L+DI ++VQC ICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN
Sbjct: 78  SSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 137

Query: 155 ECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQT 214
           ECP CR HCASRRSLRDDPN+D LIA L+ +IDK+EEE              IQ SIAQ 
Sbjct: 138 ECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQIQASIAQV 197

Query: 215 LQRQTEALGRKRS--------KPSRRL--SSRGRKSFQNHIESLCFDENEDENDYDVSKN 274
            QRQ++AL +++S          SRR    SR R++ +N IE    + N+D++     K+
Sbjct: 198 SQRQSKALVKRKSVGKGTAILSRSRRSGGGSRRRRNCRN-IEQDTSEANDDDDQNKRGKD 257

Query: 275 SSSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGS 334
           SSS +     R KRS          SS+A   D   G G E  ++ +   +   +  V +
Sbjct: 258 SSSDEPCERQRKKRS-----ATQPSSSNANNNDNCAGNGTEQTHQRDSRVISPVL--VWN 317

Query: 335 SEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSM 394
           SE ++WG+GG RS+TR G  N GA  + RN R+ +L +Y+ + E  +  ELDIHL LVS+
Sbjct: 318 SELIAWGRGGTRSNTRQGNNNQGA-ISKRNARLKRLVEYLGSLEG-NSVELDIHLKLVSL 377

Query: 395 DETRIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAKELQMKLNPSTSGTLK 454
           D   +  L  PY+C RP++ +  L +YVA    L  + VE+ ++K+    +   TS    
Sbjct: 378 DTEGLLNLHEPYLCFRPTLLVKQLREYVARHLKLKAEEVELLVSKDGDTVIGNKTS---- 437

Query: 455 NPCKESIQILNEQETLSTAKLKAHRLAC-GYLV 463
               E +Q L + ET+  AKLK   ++  GY++
Sbjct: 438 ---TEKMQSLQDDETV--AKLKVDCISSNGYMI 451

[Close]

BLAST of Lsi01G003010.1 vs. TAIR 10
Match: AT1G03770.3 (RING 1B )

HSP 1 Score: 238.4 bits (607), Expect = 1.2e-62
Identity = 168/399 (42.11%), Postives = 230/399 (57.64%), Query Frame = 0


Query: 95  AGSCGRSGFSRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 154
           + S G    S ++ V L+DI ++VQC ICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN
Sbjct: 78  SSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 137

Query: 155 ECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQT 214
           ECP CR HCASRRSLRDDPN+D LIA L+ +IDK+EEE              IQ SIAQ 
Sbjct: 138 ECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQIQASIAQV 197

Query: 215 LQRQTEALGRKRS--------KPSRRL--SSRGRKSFQNHIESLCFDENEDENDYDVSKN 274
            QRQ++AL +++S          SRR    SR R++ +N IE    + N+D++     K+
Sbjct: 198 SQRQSKALVKRKSVGKGTAILSRSRRSGGGSRRRRNCRN-IEQDTSEANDDDDQNKRGKD 257

Query: 275 SSSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGS 334
           SSS +     R KRS          SS+A   D   G G E  ++ +   +   +  V +
Sbjct: 258 SSSDEPCERQRKKRS-----ATQPSSSNANNNDNCAGNGTEQTHQRDSRVISPVL--VWN 317

Query: 335 SEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSM 394
           SE ++WG+GG RS+TR G  N GA  + RN R+ +L +Y+ + E  +  ELDIHL LVS+
Sbjct: 318 SELIAWGRGGTRSNTRQGNNNQGA-ISKRNARLKRLVEYLGSLEG-NSVELDIHLKLVSL 377

Query: 395 DETRIPALQRPYICCRPSVTIGHLSQ------YVALETALPVDVVEMCMAKELQMKLNPS 454
           D   +  L  PY+C RP++ +  L +      YVA    L  + VE+ ++K+    +   
Sbjct: 378 DTEGLLNLHEPYLCFRPTLLVKQLREVSSLPLYVARHLKLKAEEVELLVSKDGDTVIGNK 437

Query: 455 TSGTLKNPCKESIQILNEQETLSTAKLKAHRLAC-GYLV 463
           TS        E +Q L + ET+  AKLK   ++  GY++
Sbjct: 438 TS-------TEKMQSLQDDETV--AKLKVDCISSNGYMI 457

[Close]

BLAST of Lsi01G003010.1 vs. TAIR 10
Match: AT1G03770.2 (RING 1B )

HSP 1 Score: 238.4 bits (607), Expect = 1.2e-62
Identity = 168/399 (42.11%), Postives = 230/399 (57.64%), Query Frame = 0


Query: 95  AGSCGRSGFSRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 154
           + S G    S ++ V L+DI ++VQC ICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN
Sbjct: 80  SSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDKSMRLGNN 139

Query: 155 ECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQT 214
           ECP CR HCASRRSLRDDPN+D LIA L+ +IDK+EEE              IQ SIAQ 
Sbjct: 140 ECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQIQASIAQV 199

Query: 215 LQRQTEALGRKRS--------KPSRRL--SSRGRKSFQNHIESLCFDENEDENDYDVSKN 274
            QRQ++AL +++S          SRR    SR R++ +N IE    + N+D++     K+
Sbjct: 200 SQRQSKALVKRKSVGKGTAILSRSRRSGGGSRRRRNCRN-IEQDTSEANDDDDQNKRGKD 259

Query: 275 SSSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGS 334
           SSS +     R KRS          SS+A   D   G G E  ++ +   +   +  V +
Sbjct: 260 SSSDEPCERQRKKRS-----ATQPSSSNANNNDNCAGNGTEQTHQRDSRVISPVL--VWN 319

Query: 335 SEKLSWGKGGIRSHTRYGGINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVSM 394
           SE ++WG+GG RS+TR G  N GA  + RN R+ +L +Y+ + E  +  ELDIHL LVS+
Sbjct: 320 SELIAWGRGGTRSNTRQGNNNQGA-ISKRNARLKRLVEYLGSLEG-NSVELDIHLKLVSL 379

Query: 395 DETRIPALQRPYICCRPSVTIGHLSQ------YVALETALPVDVVEMCMAKELQMKLNPS 454
           D   +  L  PY+C RP++ +  L +      YVA    L  + VE+ ++K+    +   
Sbjct: 380 DTEGLLNLHEPYLCFRPTLLVKQLREVSSLPLYVARHLKLKAEEVELLVSKDGDTVIGNK 439

Query: 455 TSGTLKNPCKESIQILNEQETLSTAKLKAHRLAC-GYLV 463
           TS        E +Q L + ET+  AKLK   ++  GY++
Sbjct: 440 TS-------TEKMQSLQDDETV--AKLKVDCISSNGYMI 459

[Close]

BLAST of Lsi01G003010.1 vs. TAIR 10
Match: AT5G44280.2 (RING 1A )

HSP 1 Score: 217.2 bits (552), Expect = 2.8e-56
Identity = 147/387 (37.98%), Postives = 220/387 (56.85%), Query Frame = 0


Query: 104 SRFIAVKLSDIRREVQCPICLGIIRKTRTVMECLHRFCRECIDKSMR----LGNNECPAC 163
           S F+ + L +IR++VQCPICL  ++  R  +   ++  ++C   S +      NNECPAC
Sbjct: 120 SEFMEIDLGEIRKDVQCPICLVGMKFLRLFIS-YYKENKDCDGMSPQFCATFKNNECPAC 179

Query: 164 RTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEE--------------IQTSIAQTLQRQT 223
           R HCASRRSLRDDP +D LIA L+ +ID YEEE              IQ SIAQ  QRQ+
Sbjct: 180 RKHCASRRSLRDDPKFDALIAALFTNIDSYEEEELAFHEDEMARNKQIQASIAQISQRQS 239

Query: 224 EALGRKRS---------------KPSRRLSSRGRKSFQNHIESLCFDENEDENDYDVSKN 283
           EAL ++RS                 SRR  +      QN  E+   D+N+D N+    K+
Sbjct: 240 EALVKRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQQNASEAHEDDDNDDNNNRGRDKD 299

Query: 284 SSSADERMDTRPKRSRRGGPVRFSQSSSATGADGADGGGIENDYEVNKEKVGASIGFVGS 343
           SSS +   + R K+ R+    R +Q  S++GA+  +G   +ND EV ++  G S G V +
Sbjct: 300 SSSDERGTEVRQKKRRKRSTSRSTQHPSSSGANKNNGNCADNDTEVYRDTKGISPGLVWN 359

Query: 344 SEKLSWGKGGIRSHTRYG-GINGGAGKNSRNNRIMKLSDYVRNSENVSEEELDIHLVLVS 403
            E L+WG+GG RS+TR+G   +GG+ K+ RN R+ KL +Y+R+S + S  ELDIH+ LVS
Sbjct: 360 PEILAWGRGGTRSNTRHGNNTSGGSSKSVRNARVNKLVEYLRSSVDGSSVELDIHVKLVS 419

Query: 404 MDETRIPALQRPYICCRPSVTIGHLSQYVALETALPVDVVEMCMAK-----ELQMKLNPS 452
           +D   IP L +PY+CCRP++ +  L ++VAL+  L  + VE+ + +     +  ++  P+
Sbjct: 420 LDTKCIPDLPQPYLCCRPTLLVKQLREFVALQIHLKTEEVELLVTRRRVGEDAAIENLPA 479

The following BLAST results are available for this feature:

BLAST of Lsi01G003010.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FKW0	3.4e-75	44.65	Putative E3 ubiquitin-protein ligase RING1a OS=Arabidopsis thaliana OX=3702 GN=R...	[more]
Q0WX00	1.8e-63	42.75	Putative E3 ubiquitin-protein ligase RING1b OS=Arabidopsis thaliana OX=3702 GN=R...	[more]
Q5TJF3	9.2e-20	33.50	E3 ubiquitin-protein ligase RING1 OS=Canis lupus familiaris OX=9615 GN=RING1 PE=...	[more]
A1YER5	9.2e-20	33.50	E3 ubiquitin-protein ligase RING1 OS=Gorilla gorilla gorilla OX=9595 GN=RING1 PE...	[more]
Q06587	2.7e-19	32.99	E3 ubiquitin-protein ligase RING1 OS=Homo sapiens OX=9606 GN=RING1 PE=1 SV=2	[more]

BLAST of Lsi01G003010.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EFU8	2.5e-190	74.24	putative E3 ubiquitin-protein ligase RING1a isoform X1 OS=Cucurbita moschata OX=...	[more]
A0A5D3CNZ3	2.4e-188	73.78	Putative E3 ubiquitin-protein ligase RING1a OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A0A0KLI2	8.5e-186	75.94	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517990 P...	[more]
A0A6J1IQZ1	7.2e-185	73.28	putative E3 ubiquitin-protein ligase RING1a isoform X1 OS=Cucurbita maxima OX=36...	[more]
A0A6J1ECT6	1.5e-182	74.21	putative E3 ubiquitin-protein ligase RING1a isoform X2 OS=Cucurbita moschata OX=...	[more]

BLAST of Lsi01G003010.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881714.1	2.0e-197	78.99	putative E3 ubiquitin-protein ligase RING1a isoform X1 [Benincasa hispida]	[more]
XP_023543673.1	2.1e-191	74.44	putative E3 ubiquitin-protein ligase RING1a isoform X1 [Cucurbita pepo subsp. pe...	[more]
XP_022925653.1	5.3e-190	74.24	putative E3 ubiquitin-protein ligase RING1a isoform X1 [Cucurbita moschata]	[more]
KAG7034603.1	5.8e-189	70.06	putative E3 ubiquitin-protein ligase RING1a, partial [Cucurbita argyrosperma sub...	[more]
TYK13150.1	4.9e-188	73.78	putative E3 ubiquitin-protein ligase RING1a [Cucumis melo var. makuwa]	[more]

BLAST of Lsi01G003010.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G44280.1	2.4e-76	44.65	RING 1A	[more]
AT1G03770.1	1.3e-64	42.75	RING 1B	[more]
AT1G03770.3	1.2e-62	42.11	RING 1B	[more]
AT1G03770.2	1.2e-62	42.11	RING 1B	[more]
AT5G44280.2	2.8e-56	37.98	RING 1A	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 120..159 e-value: 9.7E-7 score: 38.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 120..160 score: 12.076194
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 104..208 e-value: 2.9E-18 score: 67.7
None	No IPR available	PFAM	PF13923	zf-C3HC4_2	coord: 119..159 e-value: 9.8E-12 score: 44.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 204..226
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 24..49
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 245..266
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 241..292
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 33..49
None	No IPR available	PANTHER	PTHR46537:SF3	OS11G0578200 PROTEIN	coord: 1..452
None	No IPR available	CDD	cd16531	RING-HC_RING1_like	coord: 120..160 e-value: 4.21225E-21 score: 84.0207
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 111..192
IPR044592	Putative E3 ubiquitin-protein ligase RING1a/b	PANTHER	PTHR46537	OS11G0578200 PROTEIN	coord: 1..452
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 136..145

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi01G003010	Lsi01G003010	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi01G003010.1.exon.9	Lsi01G003010.1.exon.9	exon
Lsi01G003010.1.exon.8	Lsi01G003010.1.exon.8	exon
Lsi01G003010.1.exon.7	Lsi01G003010.1.exon.7	exon
Lsi01G003010.1.exon.6	Lsi01G003010.1.exon.6	exon
Lsi01G003010.1.exon.5	Lsi01G003010.1.exon.5	exon
Lsi01G003010.1.exon.4	Lsi01G003010.1.exon.4	exon
Lsi01G003010.1.exon.3	Lsi01G003010.1.exon.3	exon
Lsi01G003010.1.exon.2	Lsi01G003010.1.exon.2	exon
Lsi01G003010.1.exon.1	Lsi01G003010.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi01G003010.1.CDS.9	Lsi01G003010.1.CDS.9	CDS
Lsi01G003010.1.CDS.8	Lsi01G003010.1.CDS.8	CDS
Lsi01G003010.1.CDS.7	Lsi01G003010.1.CDS.7	CDS
Lsi01G003010.1.CDS.6	Lsi01G003010.1.CDS.6	CDS
Lsi01G003010.1.CDS.5	Lsi01G003010.1.CDS.5	CDS
Lsi01G003010.1.CDS.4	Lsi01G003010.1.CDS.4	CDS
Lsi01G003010.1.CDS.3	Lsi01G003010.1.CDS.3	CDS
Lsi01G003010.1.CDS.2	Lsi01G003010.1.CDS.2	CDS
Lsi01G003010.1.CDS.1	Lsi01G003010.1.CDS.1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi01G003010.1.five_prime_UTR.1	Lsi01G003010.1.five_prime_UTR.1	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi01G003010.1	Lsi01G003010.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding