Lcy03g013350 (gene) Sponge gourd (P93075) v1

BLAST of Lcy03g013350 vs. ExPASy Swiss-Prot
Match: O48786 (Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 1.0e-216
Identity = 370/519 (71.29%), Postives = 433/519 (83.43%), Query Frame = 0

Query: 16  KVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASS 75
           KV VL  ++ ++  K    LWW+PR+IEEHF +QGIRGPPY FFIGN KELV MML ASS
Sbjct: 12  KVLVLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASS 71

Query: 76  HPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSEFYEK 135
           HPMP FSHNILPRVLSFYHHW+KIYG+TFL+WFGP  R+ V+DPD+IREI  + SEFYEK
Sbjct: 72  HPMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIF-SKSEFYEK 131

Query: 136 NEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSAT 195
           NEAHPLVKQLEGDGLL+LK +KWA HRKIISPTFHMENLKLL+PVV +SV DMV+KWS  
Sbjct: 132 NEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDK 191

Query: 196 ADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFI 255
             +  +GEVE++V++ FQ LTEDVI++TAFGSSYEDG+A+F+LQ QQM+L +EAFQKVFI
Sbjct: 192 LSE--NGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFI 251

Query: 256 PGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGP--KDLLGLMIQAS 315
           PGYRF PTR N+ SWKLDKEI+KSL+KLIE R+ N+      + K P  KDLLGLMIQA 
Sbjct: 252 PGYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQA- 311

Query: 316 NSSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSV 375
                     N+TV DIVEECKSFFFAGKQTTSNLLTWTT+LL+MHP+WQ +ARDEVL V
Sbjct: 312 ---------KNVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRV 371

Query: 376 CGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGTEFLIPI 435
           CG R +P++D V KLK LSMILNESLRLYPP VATIRRAK DV+LGG  IP GTE LIPI
Sbjct: 372 CGSRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPI 431

Query: 436 LAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAM 495
           +AVHHDQ+IWGND +EFNP RF++GV RA  HP GFIPFGLGVRTCIGQNLA+LQAKL +
Sbjct: 432 IAVHHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTL 491

Query: 496 TIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIS 533
            +++QRF+F LAPTYQHAPTVLMLLYPQ+GAPI F++++
Sbjct: 492 AVMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRLT 516

BLAST of Lcy03g013350 vs. ExPASy Swiss-Prot
Match: B9X287 (Cytochrome P450 734A6 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A6 PE=2 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 2.0e-188
Identity = 321/515 (62.33%), Postives = 405/515 (78.64%), Query Frame = 0

Query: 22  AVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSHPM--P 81
           A    +  K+   LWW+PRR+EEHF RQGI GP YRF +G  +E+V++M+AAS+ PM  P
Sbjct: 12  AAAAWVAVKVLEVLWWRPRRVEEHFARQGITGPRYRFLVGCVREMVALMVAASAKPMPPP 71

Query: 82  NFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSEFYEKNEAH 141
             SHN+LPRVL+FYHHWKKIYGSTFLIWFGP  R+A++DP++IRE+L   ++ +++ E+H
Sbjct: 72  YRSHNVLPRVLAFYHHWKKIYGSTFLIWFGPTPRLAIADPELIREVLLARADRFDRYESH 131

Query: 142 PLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSATADDT 201
           P+V+QLEG+GL++L+  KWA HR++++P FHM+NL+LL+P V  +V+DM +KW A A+  
Sbjct: 132 PMVRQLEGEGLVSLRGDKWAHHRRVLTPAFHMDNLRLLLPCVGMTVLDMADKWRAMAEAD 191

Query: 202 ASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFIPGYR 261
            SGEVEI+V D FQ +TED IT+TAFG SYEDGK +FKLQ Q M  ASEAF+KVFIPGYR
Sbjct: 192 KSGEVEIDVSDWFQVVTEDAITRTAFGRSYEDGKVVFKLQAQLMAFASEAFRKVFIPGYR 251

Query: 262 FLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQASNSSSCS 321
           FLPT+ N SSWKLDKEI+K+L+ LI GR+  +  +  +D    KDLLGLMI A+ SS+  
Sbjct: 252 FLPTKKNTSSWKLDKEIRKNLVTLI-GRRQEAGDDEKLDGCA-KDLLGLMINAAASSNGG 311

Query: 322 SLN----NNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSVCG 381
             +    + ITV+DIVEECK+FFFAGKQTTSNLLTW  V+LAMHP+WQ +AR EVL VCG
Sbjct: 312 KRSALPVSPITVNDIVEECKTFFFAGKQTTSNLLTWAIVVLAMHPEWQERARQEVLDVCG 371

Query: 382 PRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRM-IPRGTEFLIPIL 441
              +PSR+ ++KLK L MILNE+LRLYPP VAT+RRAK DVELGG + IPR TE LIPI+
Sbjct: 372 ADGVPSREQLAKLKTLGMILNETLRLYPPAVATVRRAKADVELGGYLRIPRDTELLIPIM 431

Query: 442 AVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAMT 501
           AVHHD  +WG DA++FNP RF+ GV RA  HPA FIPFGLG R CIGQNLA+L+AKL + 
Sbjct: 432 AVHHDARLWGPDAAQFNPARFAGGVARAARHPAAFIPFGLGARMCIGQNLAILEAKLTVA 491

Query: 502 IILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQ 530
           +IL RF FRL+  Y HAPTVLMLL+PQYGAPI+F+
Sbjct: 492 VILHRFEFRLSARYVHAPTVLMLLHPQYGAPIVFR 524

BLAST of Lcy03g013350 vs. ExPASy Swiss-Prot
Match: Q6Z6D6 (Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 1.1e-175
Identity = 298/529 (56.33%), Postives = 388/529 (73.35%), Query Frame = 0

Query: 23  VVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSHPM-PNF 82
           V + + A+MA ALWW+PRR+E HF  QG+RGPPYRF +G+ +E+V++M  ASS PM P  
Sbjct: 29  VTMHVAARMADALWWRPRRLEAHFAAQGVRGPPYRFLLGSVREMVALMAEASSKPMSPPT 88

Query: 83  SHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSEFYEKNEAHPL 142
           SHN LPRVL+FYH+W+KIYG  FLIWFGP  R+ V++P++IREI  T ++ +++ EAHP+
Sbjct: 89  SHNALPRVLAFYHYWRKIYGHRFLIWFGPTPRLTVAEPELIREIFLTRADAFDRYEAHPV 148

Query: 143 VKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSATADDTAS 202
           V+QLEGDGL++L   KWALHR++++  F+ +NL  LIP V +SV  +  KW A A+   S
Sbjct: 149 VRQLEGDGLVSLHGDKWALHRRVLTDAFYPDNLNRLIPHVGKSVAALAAKWGAMAEAGGS 208

Query: 203 GEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFIPGYRFL 262
           GEVE++V + FQ +TE+ IT+  FG SY+DG+ +F +Q Q M  ASEAF+KV +PGYRFL
Sbjct: 209 GEVEVDVAEWFQAVTEEAITRATFGRSYDDGRVVFAMQGQLMAFASEAFRKVLVPGYRFL 268

Query: 263 PTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQASNSSSC--- 322
           PT+ N  SW+LD+EI++SLM+LI  R   +E+    D+   +DLLGLMI A  +++    
Sbjct: 269 PTKKNRLSWRLDREIRRSLMRLIGRRSDEAEQGEKADDGSFRDLLGLMINAGAAAATRGN 328

Query: 323 -----SSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSV 382
                +S    I V D++EECK+FFFAGKQTT+NLLTW TVLLAMHP WQ +AR EV  V
Sbjct: 329 AGGEKNSPAAAIPVEDMLEECKTFFFAGKQTTTNLLTWATVLLAMHPDWQERARREVFDV 388

Query: 383 CGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELG-GRMIPRGTEFLIP 442
           CG   LPS++ + KLK L MI+NE+LRLYPP VATIRRAK DV+L  G MIPR  E L+P
Sbjct: 389 CGAGELPSKEHLPKLKTLGMIMNETLRLYPPAVATIRRAKVDVQLSDGCMIPRDMELLVP 448

Query: 443 ILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLA 502
           I+A+HHD   WG DAS+FNP RF+ G  +A  HP  FIPFGLG R C+GQNLA L+AKL 
Sbjct: 449 IMAIHHDTRYWGPDASQFNPARFANGASKAAKHPLAFIPFGLGSRMCVGQNLARLEAKLT 508

Query: 503 MTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKISQFTGDASMP 542
           M I+LQRF  R +P Y HAPTVLMLLYPQYGAP+IF+ +S    D++ P
Sbjct: 509 MAILLQRFEIRTSPNYVHAPTVLMLLYPQYGAPLIFRPLSSHPPDSTGP 557

BLAST of Lcy03g013350 vs. ExPASy Swiss-Prot
Match: Q69XM6 (Cytochrome P450 734A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A4 PE=2 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 6.6e-168
Identity = 292/537 (54.38%), Postives = 386/537 (71.88%), Query Frame = 0

Query: 12  LESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMML 71
           +E+V V     +++ + A++A A+WW+PRR+E HF  QG+RGPPYRF +G  +E+V++M 
Sbjct: 2   MEAVAVAAAVLLLLHVAARVADAVWWRPRRLEAHFAGQGVRGPPYRFLVGCVREMVALMA 61

Query: 72  AASSHPMPNFS-HNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNS 131
            A++ PMP  + HN LPRVL+FYH+W+KIYG TFLIWFGP  R+ V++P+M+REI  T +
Sbjct: 62  EATAKPMPPAAPHNALPRVLAFYHYWRKIYGPTFLIWFGPTPRLTVAEPEMVREIFLTRA 121

Query: 132 EFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVE 191
           E +++ EAHP+V+QLEGDGL++L   KWA HR++++P F+ +NL  L+P V  SV  + E
Sbjct: 122 EAFDRYEAHPVVRQLEGDGLVSLHGDKWAHHRRVLTPGFYPDNLNRLVPHVGRSVAALAE 181

Query: 192 KWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAF 251
           +W A A     GEVE++V + FQ + E+ IT+  FG SY+ G+ +F+LQ + M  ASEAF
Sbjct: 182 RWRAMA-CAGGGEVEVDVAEWFQAVAEEAITRATFGRSYDSGRVVFRLQARLMAFASEAF 241

Query: 252 QKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKG-----NSEKNWAMDEK---GP 311
           +KV +PGYRFLPT+ N  SW LD+EI++ L++LI  R G       E    + +K   G 
Sbjct: 242 RKVLVPGYRFLPTKKNRMSWGLDREIRRGLVRLIGRRSGGDGGEEDETTTELKDKQDSGF 301

Query: 312 KDLLGLMIQASNSSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQW 371
            DLLGLMI A        ++  + V D+VEECK+FFFAGKQTT+NLLTW TVLLAMHP W
Sbjct: 302 NDLLGLMINA-------GVDRTMPVEDMVEECKTFFFAGKQTTTNLLTWATVLLAMHPDW 361

Query: 372 QIQARDEVLSVCGPRA--LPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGG 431
           Q +AR EVL+VCG  A  LP++D + KLK L MILNE+LRLYPP VATIRRAK DV LGG
Sbjct: 362 QDRARREVLAVCGDAAGELPTKDHLPKLKTLGMILNETLRLYPPAVATIRRAKFDVTLGG 421

Query: 432 R--------MIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPF 491
                     IPR TE L+PI+A+HHD  +WG DA++FNP RF+ G  RA  HP  FIPF
Sbjct: 422 GGDGDAGGIHIPRDTELLVPIMAIHHDARLWGPDAAQFNPARFASGAARAAKHPLAFIPF 481

Query: 492 GLGVRTCIGQNLALLQAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQ 530
           GLG R CIGQ+LA+L+AKL M ++LQRF   L+PTY HAPTVLMLL+PQYGAP+IF+
Sbjct: 482 GLGSRMCIGQSLAILEAKLTMAVLLQRFDLALSPTYVHAPTVLMLLHPQYGAPLIFR 530

BLAST of Lcy03g013350 vs. ExPASy Swiss-Prot
Match: Q8LIF2 (Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A5 PE=2 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 1.3e-150
Identity = 261/512 (50.98%), Postives = 360/512 (70.31%), Query Frame = 0

Query: 24  VVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSHPM-PNFS 83
           V    A+ A ALWW+PRR+E HF  QG+RGP YRFF+G+  ELV +M+ A+S PM P  S
Sbjct: 28  VYAAAARAAEALWWRPRRVERHFAAQGVRGPGYRFFVGSSIELVRLMVDAASRPMEPPTS 87

Query: 84  HNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSEFYEKNEAHPLV 143
           H+ILPRVL FYHHW+K+YG   LIWFG   R+ VS+P++IRE+L T ++ +++ EAHP++
Sbjct: 88  HDILPRVLPFYHHWRKLYGPMHLIWFGRTPRLVVSEPELIREVLLTRADHFDRYEAHPMI 147

Query: 144 KQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSATADDTASG 203
            Q EG GL  L  ++WA  R++++P FH ENL+++ P V  +V  M+++ +  A    +G
Sbjct: 148 CQFEGYGLSNLHGERWARRRRVLTPAFHTENLRMIAPFVAGTVTRMLDELAERARAGGAG 207

Query: 204 EVEIEVFDCFQTLTEDVITKTAFG-SSYEDGKAIFKLQTQQMILASEAFQKVFIPGYRFL 263
           E E++V + FQ + ++ IT  AFG  +Y+DG A+F+LQ +    A+EA  KV+IPGYRFL
Sbjct: 208 EAEVDVAEWFQRVPQEAITFAAFGRRNYDDGAAVFRLQDELAGYATEAHSKVYIPGYRFL 267

Query: 264 PTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQASNSSSCSSL 323
           PTR N   W+LD+EI+  L K + G +  S  +    + G     G+          S +
Sbjct: 268 PTRKNRRVWQLDREIRSHLAKFVTGLQSCSSSHGDDADDGGDGGGGM------REFMSFM 327

Query: 324 NNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSVCGPRALPS 383
              +T  +I+EE K+FFFAGK+T SNLLTWTTV LAMHP+WQ +AR EV++VCG   LP+
Sbjct: 328 APAMTAGEIIEESKNFFFAGKETLSNLLTWTTVALAMHPEWQERARREVVAVCGRGDLPT 387

Query: 384 RDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGTEFLIPILAVHHDQS 443
           +D + KLK L MILNE+LRLYPP VA IR AKEDVELGG ++P GTE +IPI+AVHHD +
Sbjct: 388 KDHLPKLKTLGMILNETLRLYPPAVAMIRTAKEDVELGGCVVPAGTEVMIPIMAVHHDAA 447

Query: 444 IWGNDASEFNPGRF-SEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAMTIILQRF 503
            WG+DA+EFNP RF ++  G    HP  F+PFG G R CIGQN+AL++AK+A+ ++L+RF
Sbjct: 448 AWGDDAAEFNPARFAADDDGGRRRHPMAFMPFGGGARVCIGQNMALMEAKVALAVVLRRF 507

Query: 504 SFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIS 533
            FRL+P Y HAP VLM+L PQ+GAP+IF+ ++
Sbjct: 508 EFRLSPAYVHAPRVLMILSPQFGAPVIFRPLT 533

BLAST of Lcy03g013350 vs. ExPASy TrEMBL
Match: A0A6J1L528 (cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111499208 PE=3 SV=1)

HSP 1 Score: 941.8 bits (2433), Expect = 1.3e-270
Identity = 465/530 (87.74%), Postives = 493/530 (93.02%), Query Frame = 0

Query: 9   PAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVS 68
           P P +S+K+P LCA++ IL  KMAVALWWKPRRIE HFLRQGIRGPPYRFFIGNFKEL S
Sbjct: 6   PPPFDSIKIPFLCALLFILAVKMAVALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDS 65

Query: 69  MMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTT 128
           MMLAA+S PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGP+VRVAVSDPDMIR+ILTT
Sbjct: 66  MMLAATSRPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTT 125

Query: 129 NSEFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDM 188
           NSEFYEKNEAHPL+K+LEGDGLLTLKDQKWALHRKIISP F+MENLKLLIPVVEESVVDM
Sbjct: 126 NSEFYEKNEAHPLIKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDM 185

Query: 189 VEKWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASE 248
           VEKWSA A   AS EVEI+VFD FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILASE
Sbjct: 186 VEKWSAMAGGAASDEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASE 245

Query: 249 AFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGL 308
           AFQKVFIPGYRFLPTRTNI+SWKLDKEI+KSLMKLIE RKGNSEK WAMDEK PKDLLGL
Sbjct: 246 AFQKVFIPGYRFLPTRTNINSWKLDKEIRKSLMKLIESRKGNSEKIWAMDEKAPKDLLGL 305

Query: 309 MIQASN-SSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 368
           MIQASN SSS SSL+ NI+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR
Sbjct: 306 MIQASNSSSSSSSLHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 365

Query: 369 DEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGT 428
           DEVLSVCGPRALPSRDD+SK KILSMILNESLRLYPPTVATIRRAK  VEL GRMIPRGT
Sbjct: 366 DEVLSVCGPRALPSRDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMIPRGT 425

Query: 429 EFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALL 488
           EFLIPILAVHHDQ+IWGNDASEFNPGRFSEGVG AGNHPAGFIPFG G+R CIG+NLALL
Sbjct: 426 EFLIPILAVHHDQNIWGNDASEFNPGRFSEGVGHAGNHPAGFIPFGHGLRACIGKNLALL 485

Query: 489 QAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKISQFTGD 538
           QAKLA+ II+QRFSFRLAP+YQHAP VLMLLYPQYGAP++FQKI +   D
Sbjct: 486 QAKLAIAIIVQRFSFRLAPSYQHAPAVLMLLYPQYGAPVVFQKIVETRRD 535

BLAST of Lcy03g013350 vs. ExPASy TrEMBL
Match: A0A6J1EZI1 (cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111440975 PE=3 SV=1)

HSP 1 Score: 937.6 bits (2422), Expect = 2.4e-269
Identity = 462/524 (88.17%), Postives = 491/524 (93.70%), Query Frame = 0

Query: 9   PAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVS 68
           P PL+S+K+P LCA++ IL  KM  ALWWKPRRIE HFLRQGIRGPPYRFFIGNFKEL S
Sbjct: 6   PPPLDSIKIPFLCALLFILAVKMGAALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDS 65

Query: 69  MMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTT 128
           MMLAA+S PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGP+VRVAVSDPDMIR+ILTT
Sbjct: 66  MMLAATSRPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTT 125

Query: 129 NSEFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDM 188
           NSEFYEKNEAHPL+K+LEGDGLLTLKDQKWALHRKIISP F+MENLKLLIPVVEESVVDM
Sbjct: 126 NSEFYEKNEAHPLIKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDM 185

Query: 189 VEKWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASE 248
           VEKWSA A   AS EVEI+VFD FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILASE
Sbjct: 186 VEKWSAMAGGGASDEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASE 245

Query: 249 AFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGL 308
           AFQKVFIPGYRFLPTRTNI+SWKLDKEI+KSLMKLIEGRKGNS+K WAMDEK PKDLLGL
Sbjct: 246 AFQKVFIPGYRFLPTRTNINSWKLDKEIRKSLMKLIEGRKGNSQKIWAMDEKAPKDLLGL 305

Query: 309 MIQASN-SSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 368
           MIQASN SSS SSL+ NI+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR
Sbjct: 306 MIQASNSSSSSSSLHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 365

Query: 369 DEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGT 428
           DEVLSVCGPRALPS DD+SK KILSMILNESLRLYPPTVATIRRAK  VEL GRMIPRGT
Sbjct: 366 DEVLSVCGPRALPSTDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMIPRGT 425

Query: 429 EFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALL 488
           EFLIPILAVHHDQ+IWGNDASEFNPGRFSEGVG AGNHPAGFIPFG G+R CIG+NLALL
Sbjct: 426 EFLIPILAVHHDQNIWGNDASEFNPGRFSEGVGHAGNHPAGFIPFGHGLRACIGKNLALL 485

Query: 489 QAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 532
           QAKLA+ II+QRFSFRLAP+YQHAPTVLMLLYPQYGAP++F+KI
Sbjct: 486 QAKLAIAIIVQRFSFRLAPSYQHAPTVLMLLYPQYGAPVVFRKI 529

BLAST of Lcy03g013350 vs. ExPASy TrEMBL
Match: A0A1S3CMW0 (cytochrome P450 734A1-like OS=Cucumis melo OX=3656 GN=LOC103502730 PE=3 SV=1)

HSP 1 Score: 899.8 bits (2324), Expect = 5.5e-258
Identity = 446/537 (83.05%), Postives = 487/537 (90.69%), Query Frame = 0

Query: 1   MKENPAPAPAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFI 60
           MKE    +PA   +  V     ++ I+ AK+ + LWWKP++IE+HF RQGI+GPPYRFFI
Sbjct: 1   MKEVKIESPA-WSAAAVTTAVLLIFIISAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFI 60

Query: 61  GNFKELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPD 120
           GNFKELVS+MLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGP+VRVAVSDPD
Sbjct: 61  GNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPD 120

Query: 121 MIREILTTNSEFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPV 180
           MIREI  TNS++YEKNEAHPLVKQLEGDGLLTL  QKWALHRKIISPTFH++NLKLLIP+
Sbjct: 121 MIREIFATNSQYYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPL 180

Query: 181 VEESVVDMVEKWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQT 240
           VEESVV+MVEKW + A+   S EVEI+V+D FQTLTEDVITKTAFGSSYEDGKAIFKLQ 
Sbjct: 181 VEESVVEMVEKWWSMAESVESSEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQN 240

Query: 241 QQMILASEAFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSE-KNWAMDE 300
           QQMILAS+AFQKVFIPGYRFLPTRTNI+SWKLDKEIKKSLMKLIEGRKGN E KNW M+E
Sbjct: 241 QQMILASQAFQKVFIPGYRFLPTRTNINSWKLDKEIKKSLMKLIEGRKGNLEKKNWGMNE 300

Query: 301 KGPKDLLGLMIQAS-----NSSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTV 360
           K PKDLLGLMIQAS     N+++ ++ N++ITVHDIVEECKSFFFAGKQTTSNLLTWTTV
Sbjct: 301 KRPKDLLGLMIQASLLNNNNNNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTV 360

Query: 361 LLAMHPQWQIQARDEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKE 420
           LLAMHPQWQIQARDEV++VCGPRALPS+ DVS  KILSMILNESLRLYPPTVATIRRAK 
Sbjct: 361 LLAMHPQWQIQARDEVINVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKA 420

Query: 421 DVELGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGL 480
           DVEL GRMIPRGTEFLIPI+AVHHDQ+IWGNDASEFNPGRFSEGV RAGNHPAGFIPFGL
Sbjct: 421 DVELAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSRAGNHPAGFIPFGL 480

Query: 481 GVRTCIGQNLALLQAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 532
           GVRTCIGQNLA+L+AKL M IILQRF+FRLAPTYQHAPTVLMLLYPQYGAPIIFQKI
Sbjct: 481 GVRTCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 536

BLAST of Lcy03g013350 vs. ExPASy TrEMBL
Match: A0A0A0LU20 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G305710 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.7e-257
Identity = 445/532 (83.65%), Postives = 486/532 (91.35%), Query Frame = 0

Query: 1   MKENPAPAPAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFI 60
           MKE    +PA   +V V ++  ++ I+ AK+ + LWWKP++IE+HF RQGI+GPPYRFFI
Sbjct: 1   MKEVKIESPAWSATV-VTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFI 60

Query: 61  GNFKELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPD 120
           GNFKELVS+MLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGP+VRVAVSDPD
Sbjct: 61  GNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPD 120

Query: 121 MIREILTTNSEFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPV 180
           MIREI  TNS FYEKNEAHPLVKQLEGDGLLTL  QKWALHRKIISPTFH++NLKLLIP+
Sbjct: 121 MIREIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPL 180

Query: 181 VEESVVDMVEKWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQT 240
           VEESVV+MVEKW + A+   SGEVEI+V+D FQTLTEDVITKTAFGSSYEDGKAIFKLQT
Sbjct: 181 VEESVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQT 240

Query: 241 QQMILASEAFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSE-KNWAMDE 300
           QQMILAS+AFQKVFIPGYRFLPTRTNI+SWKLD+EIKKSLMKLIEGRK N E KNW M+E
Sbjct: 241 QQMILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGMNE 300

Query: 301 KGPKDLLGLMIQASNSSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMH 360
           K PKDLLGLMIQAS  ++ ++ N++ITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMH
Sbjct: 301 KRPKDLLGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMH 360

Query: 361 PQWQIQARDEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELG 420
           PQWQIQARDEV +VCGPRALPS+ DVS  KILSMILNESLRLYPPTVATIRRAK DVEL 
Sbjct: 361 PQWQIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVELA 420

Query: 421 GRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTC 480
           GRMIPRGTEFLIPI+AVHHDQ+IWGNDASEFNPGRFSEGV +A NHPAGFIPFGLGVRTC
Sbjct: 421 GRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVRTC 480

Query: 481 IGQNLALLQAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 532
           IGQNLALL+AKL + IILQRF+FRLAPTYQHAPTVLMLLYPQYGAPIIFQKI
Sbjct: 481 IGQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 531

BLAST of Lcy03g013350 vs. ExPASy TrEMBL
Match: A0A6J1H9G1 (cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111461725 PE=3 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 2.8e-254
Identity = 436/516 (84.50%), Postives = 465/516 (90.12%), Query Frame = 0

Query: 17  VPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSH 76
           V     VVVIL AKM + LWWKPRRIE HFLRQGI GPPYRFFIGNFKELVSMM AASSH
Sbjct: 15  VAAAAVVVVILGAKMGLVLWWKPRRIENHFLRQGIGGPPYRFFIGNFKELVSMMAAASSH 74

Query: 77  PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSEFYEKN 136
           P+PNFSHNILPRVLSFYHHWKKIYGSTF+IWFGP+ RVAVSDPDMIREI  TNS FYEKN
Sbjct: 75  PIPNFSHNILPRVLSFYHHWKKIYGSTFVIWFGPIARVAVSDPDMIREIFITNSHFYEKN 134

Query: 137 EAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSATA 196
           EAHPLVKQLEGDGLLTL   KWALHRKIISPTFH +NLKLLIP+VEESV+DMV+KWS+  
Sbjct: 135 EAHPLVKQLEGDGLLTLNGPKWALHRKIISPTFHTDNLKLLIPLVEESVMDMVQKWSSMT 194

Query: 197 DDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFIP 256
           ++T SGEVEI+VFD FQTLTEDVITKTAFG SYEDGKAIFK+QTQQMILASEAFQKVFIP
Sbjct: 195 EETTSGEVEIDVFDSFQTLTEDVITKTAFGCSYEDGKAIFKIQTQQMILASEAFQKVFIP 254

Query: 257 GYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQASNSS 316
           GYRFLPTRTN+ SWKL+KEIKKSL+KLIEGRK N EKNWA++EKGPKDLLGLMIQAS   
Sbjct: 255 GYRFLPTRTNMKSWKLEKEIKKSLLKLIEGRKRNFEKNWAINEKGPKDLLGLMIQAS--- 314

Query: 317 SCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSVCGP 376
                   I+VHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHP+WQI AR EV ++CGP
Sbjct: 315 --------ISVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPRWQILARHEVFTICGP 374

Query: 377 RALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGTEFLIPILAV 436
           RALPS DDVSK KILSMILNESLRLYPPTVATIRRA  DVELGGRMIPRGTEFLIPILAV
Sbjct: 375 RALPSSDDVSKFKILSMILNESLRLYPPTVATIRRANADVELGGRMIPRGTEFLIPILAV 434

Query: 437 HHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAMTII 496
           HHDQ+IWGNDASEFNP RFS+GV RAGNHP GFIPFGLGVR CIGQNLA+L+AKLAM II
Sbjct: 435 HHDQTIWGNDASEFNPERFSDGVSRAGNHPVGFIPFGLGVRNCIGQNLAVLEAKLAMVII 494

Query: 497 LQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIS 533
           LQR SFRLAPTYQHAPTVLMLLYPQYGAPI+F+KI+
Sbjct: 495 LQRLSFRLAPTYQHAPTVLMLLYPQYGAPILFEKIA 519

BLAST of Lcy03g013350 vs. NCBI nr
Match: XP_023531517.1 (cytochrome P450 734A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 942.6 bits (2435), Expect = 1.5e-270
Identity = 466/528 (88.26%), Postives = 494/528 (93.56%), Query Frame = 0

Query: 9   PAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVS 68
           P PL+S+K+P LCA++ IL  KMAVALWWKPRRIE HFLRQGIRGPPYRFFIGNFKEL S
Sbjct: 6   PPPLDSIKIPFLCALLFILAVKMAVALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDS 65

Query: 69  MMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTT 128
           MMLAA+S PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGP+VRVAVSDPDMIR+ILTT
Sbjct: 66  MMLAATSRPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTT 125

Query: 129 NSEFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDM 188
           NSEFYEKNEAHPL+K+LEGDGLLTLKDQKWALHRKIISP F+MENLKLLIPVVEESVVDM
Sbjct: 126 NSEFYEKNEAHPLIKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDM 185

Query: 189 VEKWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASE 248
           VEKWSA A   AS EVEI+VFD FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILASE
Sbjct: 186 VEKWSAMAGGAASDEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASE 245

Query: 249 AFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGL 308
           AFQKVFIPGYRFLPTRTNI+SWKLDKEI+KSLMKLIEGRKGNS+K WAMDEK PKDLLGL
Sbjct: 246 AFQKVFIPGYRFLPTRTNINSWKLDKEIRKSLMKLIEGRKGNSQKIWAMDEKAPKDLLGL 305

Query: 309 MIQASN-----SSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQ 368
           MIQASN     SSS SSL+ NI+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQ
Sbjct: 306 MIQASNSSSSSSSSSSSLHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQ 365

Query: 369 IQARDEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMI 428
           IQARDEVLSVCGPRALPSRDD+SK KILSMILNESLRLYPPTVATIRRAK  VEL GRMI
Sbjct: 366 IQARDEVLSVCGPRALPSRDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMI 425

Query: 429 PRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQN 488
           PRGTEFLIPILAVHHDQ+IWGNDASEFNPGRFSEGVG AGNHPAGFIPFG G+R CIG+N
Sbjct: 426 PRGTEFLIPILAVHHDQNIWGNDASEFNPGRFSEGVGHAGNHPAGFIPFGHGLRACIGKN 485

Query: 489 LALLQAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 532
           LALLQAKLA+ II+QRFSFRLAP+YQHAPTVLMLLYPQYGAPI+F+KI
Sbjct: 486 LALLQAKLAIAIIVQRFSFRLAPSYQHAPTVLMLLYPQYGAPIVFRKI 533

BLAST of Lcy03g013350 vs. NCBI nr
Match: XP_023006493.1 (cytochrome P450 734A1-like [Cucurbita maxima])

HSP 1 Score: 941.8 bits (2433), Expect = 2.6e-270
Identity = 465/530 (87.74%), Postives = 493/530 (93.02%), Query Frame = 0

Query: 9   PAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVS 68
           P P +S+K+P LCA++ IL  KMAVALWWKPRRIE HFLRQGIRGPPYRFFIGNFKEL S
Sbjct: 6   PPPFDSIKIPFLCALLFILAVKMAVALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDS 65

Query: 69  MMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTT 128
           MMLAA+S PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGP+VRVAVSDPDMIR+ILTT
Sbjct: 66  MMLAATSRPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTT 125

Query: 129 NSEFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDM 188
           NSEFYEKNEAHPL+K+LEGDGLLTLKDQKWALHRKIISP F+MENLKLLIPVVEESVVDM
Sbjct: 126 NSEFYEKNEAHPLIKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDM 185

Query: 189 VEKWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASE 248
           VEKWSA A   AS EVEI+VFD FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILASE
Sbjct: 186 VEKWSAMAGGAASDEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASE 245

Query: 249 AFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGL 308
           AFQKVFIPGYRFLPTRTNI+SWKLDKEI+KSLMKLIE RKGNSEK WAMDEK PKDLLGL
Sbjct: 246 AFQKVFIPGYRFLPTRTNINSWKLDKEIRKSLMKLIESRKGNSEKIWAMDEKAPKDLLGL 305

Query: 309 MIQASN-SSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 368
           MIQASN SSS SSL+ NI+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR
Sbjct: 306 MIQASNSSSSSSSLHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 365

Query: 369 DEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGT 428
           DEVLSVCGPRALPSRDD+SK KILSMILNESLRLYPPTVATIRRAK  VEL GRMIPRGT
Sbjct: 366 DEVLSVCGPRALPSRDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMIPRGT 425

Query: 429 EFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALL 488
           EFLIPILAVHHDQ+IWGNDASEFNPGRFSEGVG AGNHPAGFIPFG G+R CIG+NLALL
Sbjct: 426 EFLIPILAVHHDQNIWGNDASEFNPGRFSEGVGHAGNHPAGFIPFGHGLRACIGKNLALL 485

Query: 489 QAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKISQFTGD 538
           QAKLA+ II+QRFSFRLAP+YQHAP VLMLLYPQYGAP++FQKI +   D
Sbjct: 486 QAKLAIAIIVQRFSFRLAPSYQHAPAVLMLLYPQYGAPVVFQKIVETRRD 535

BLAST of Lcy03g013350 vs. NCBI nr
Match: KAG7021795.1 (Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 937.6 bits (2422), Expect = 4.9e-269
Identity = 462/527 (87.67%), Postives = 493/527 (93.55%), Query Frame = 0

Query: 9   PAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVS 68
           P PL+S+K+P LCA++ IL  KM VALWWKPRRIE HFLRQGIRGPPYRFFIGNFKEL S
Sbjct: 6   PPPLDSIKIPFLCALLFILAVKMGVALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDS 65

Query: 69  MMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTT 128
           MMLAA+S PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGP+VRVAVSDPDMIR+ILT 
Sbjct: 66  MMLAATSRPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTN 125

Query: 129 NSEFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDM 188
           NSEFYEKNEAHPL+K+LEGDGLLTLKDQKWALHRKIISP F+MENLKLLIPVVEESVVDM
Sbjct: 126 NSEFYEKNEAHPLIKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDM 185

Query: 189 VEKWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASE 248
           VEKWSA A   AS EVEI+VFD FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILASE
Sbjct: 186 VEKWSAMAGGGASDEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASE 245

Query: 249 AFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGL 308
           AFQKVFIPGYRFLPTRTNI+SWKLDKEI+KSLMKLIEGRKGNS+K WAMDEK PKDLLGL
Sbjct: 246 AFQKVFIPGYRFLPTRTNINSWKLDKEIRKSLMKLIEGRKGNSQKIWAMDEKAPKDLLGL 305

Query: 309 MIQASN----SSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQI 368
           MIQASN    SSS SSL+ NI+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQI
Sbjct: 306 MIQASNSSSSSSSSSSLHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQI 365

Query: 369 QARDEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIP 428
           QARDEVLS+CGPRALPSRDD+SK KILSMILNESLRLYPPTVATIRRAK  VEL GRMIP
Sbjct: 366 QARDEVLSLCGPRALPSRDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMIP 425

Query: 429 RGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNL 488
           RGTEFLIPILAVHHDQ+IWGNDASEFNPGRFSEGVG+AGNHPAGFIPFG G+R CIG+NL
Sbjct: 426 RGTEFLIPILAVHHDQNIWGNDASEFNPGRFSEGVGQAGNHPAGFIPFGHGLRACIGKNL 485

Query: 489 ALLQAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 532
           ALLQAKLA+ II+QRFSFRLAP+YQHAPTVLMLLYPQYGAP++F+KI
Sbjct: 486 ALLQAKLAIAIIVQRFSFRLAPSYQHAPTVLMLLYPQYGAPVVFRKI 532

BLAST of Lcy03g013350 vs. NCBI nr
Match: XP_022933596.1 (cytochrome P450 734A1-like [Cucurbita moschata])

HSP 1 Score: 937.6 bits (2422), Expect = 4.9e-269
Identity = 462/524 (88.17%), Postives = 491/524 (93.70%), Query Frame = 0

Query: 9   PAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVS 68
           P PL+S+K+P LCA++ IL  KM  ALWWKPRRIE HFLRQGIRGPPYRFFIGNFKEL S
Sbjct: 6   PPPLDSIKIPFLCALLFILAVKMGAALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDS 65

Query: 69  MMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTT 128
           MMLAA+S PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGP+VRVAVSDPDMIR+ILTT
Sbjct: 66  MMLAATSRPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTT 125

Query: 129 NSEFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDM 188
           NSEFYEKNEAHPL+K+LEGDGLLTLKDQKWALHRKIISP F+MENLKLLIPVVEESVVDM
Sbjct: 126 NSEFYEKNEAHPLIKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDM 185

Query: 189 VEKWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASE 248
           VEKWSA A   AS EVEI+VFD FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILASE
Sbjct: 186 VEKWSAMAGGGASDEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASE 245

Query: 249 AFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGL 308
           AFQKVFIPGYRFLPTRTNI+SWKLDKEI+KSLMKLIEGRKGNS+K WAMDEK PKDLLGL
Sbjct: 246 AFQKVFIPGYRFLPTRTNINSWKLDKEIRKSLMKLIEGRKGNSQKIWAMDEKAPKDLLGL 305

Query: 309 MIQASN-SSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 368
           MIQASN SSS SSL+ NI+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR
Sbjct: 306 MIQASNSSSSSSSLHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 365

Query: 369 DEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGT 428
           DEVLSVCGPRALPS DD+SK KILSMILNESLRLYPPTVATIRRAK  VEL GRMIPRGT
Sbjct: 366 DEVLSVCGPRALPSTDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMIPRGT 425

Query: 429 EFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALL 488
           EFLIPILAVHHDQ+IWGNDASEFNPGRFSEGVG AGNHPAGFIPFG G+R CIG+NLALL
Sbjct: 426 EFLIPILAVHHDQNIWGNDASEFNPGRFSEGVGHAGNHPAGFIPFGHGLRACIGKNLALL 485

Query: 489 QAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 532
           QAKLA+ II+QRFSFRLAP+YQHAPTVLMLLYPQYGAP++F+KI
Sbjct: 486 QAKLAIAIIVQRFSFRLAPSYQHAPTVLMLLYPQYGAPVVFRKI 529

BLAST of Lcy03g013350 vs. NCBI nr
Match: XP_038880221.1 (cytochrome P450 734A1-like [Benincasa hispida])

HSP 1 Score: 931.4 bits (2406), Expect = 3.5e-267
Identity = 466/530 (87.92%), Postives = 490/530 (92.45%), Query Frame = 0

Query: 12  LESV--KVP------VLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNF 71
           +ESV  KVP      V  AVV+IL AKM + LWWKPRRIE+HF RQGIRGPPYRFFIGNF
Sbjct: 3   IESVLSKVPPVWAAAVTAAVVMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIGNF 62

Query: 72  KELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIR 131
           KE+VSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGP+VRVAVSDPDMIR
Sbjct: 63  KEVVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIR 122

Query: 132 EILTTNSEFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEE 191
           EI TTNS FYEKNEAHPLVKQLEGDGLLTLK QKWALHRKIISPTFHM+NLKLLIP+VEE
Sbjct: 123 EIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLVEE 182

Query: 192 SVVDMVEKWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQM 251
           SVV+MVEKWS+ A+ T S EVEI+VF+CFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQM
Sbjct: 183 SVVEMVEKWSSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQM 242

Query: 252 ILASEAFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPK 311
           ILAS+AF KVFIPGYRFLPTRTNI+SWKLDKEIKKSLMKLIEGRK NSEKNW MDEK PK
Sbjct: 243 ILASQAFHKVFIPGYRFLPTRTNINSWKLDKEIKKSLMKLIEGRKWNSEKNWGMDEKRPK 302

Query: 312 DLLGLMIQASNSSSCSSLNNN--ITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQ 371
           DLLGLMIQAS+    ++ NNN  ITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQ
Sbjct: 303 DLLGLMIQASSLKYNNNNNNNNSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQ 362

Query: 372 WQIQARDEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGR 431
           WQIQARDEV +VCG RALPS+ DVSK KILSMILNESLRLYPPTVATIRRAK DVELGGR
Sbjct: 363 WQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVELGGR 422

Query: 432 MIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIG 491
           MIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLG RTCIG
Sbjct: 423 MIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGARTCIG 482

Query: 492 QNLALLQAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 532
           QNLA+L+AKL M IILQRF+FRLAPTYQHAPTVLMLLYPQYGAPI+FQKI
Sbjct: 483 QNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKI 532

BLAST of Lcy03g013350 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 754.2 bits (1946), Expect = 7.2e-218
Identity = 370/519 (71.29%), Postives = 433/519 (83.43%), Query Frame = 0

Query: 16  KVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASS 75
           KV VL  ++ ++  K    LWW+PR+IEEHF +QGIRGPPY FFIGN KELV MML ASS
Sbjct: 12  KVLVLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASS 71

Query: 76  HPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSEFYEK 135
           HPMP FSHNILPRVLSFYHHW+KIYG+TFL+WFGP  R+ V+DPD+IREI  + SEFYEK
Sbjct: 72  HPMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIF-SKSEFYEK 131

Query: 136 NEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSAT 195
           NEAHPLVKQLEGDGLL+LK +KWA HRKIISPTFHMENLKLL+PVV +SV DMV+KWS  
Sbjct: 132 NEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDK 191

Query: 196 ADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFI 255
             +  +GEVE++V++ FQ LTEDVI++TAFGSSYEDG+A+F+LQ QQM+L +EAFQKVFI
Sbjct: 192 LSE--NGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFI 251

Query: 256 PGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGP--KDLLGLMIQAS 315
           PGYRF PTR N+ SWKLDKEI+KSL+KLIE R+ N+      + K P  KDLLGLMIQA 
Sbjct: 252 PGYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQA- 311

Query: 316 NSSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSV 375
                     N+TV DIVEECKSFFFAGKQTTSNLLTWTT+LL+MHP+WQ +ARDEVL V
Sbjct: 312 ---------KNVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRV 371

Query: 376 CGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGTEFLIPI 435
           CG R +P++D V KLK LSMILNESLRLYPP VATIRRAK DV+LGG  IP GTE LIPI
Sbjct: 372 CGSRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPI 431

Query: 436 LAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAM 495
           +AVHHDQ+IWGND +EFNP RF++GV RA  HP GFIPFGLGVRTCIGQNLA+LQAKL +
Sbjct: 432 IAVHHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTL 491

Query: 496 TIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIS 533
            +++QRF+F LAPTYQHAPTVLMLLYPQ+GAPI F++++
Sbjct: 492 AVMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRLT 516

BLAST of Lcy03g013350 vs. TAIR 10
Match: AT3G14620.1 (cytochrome P450, family 72, subfamily A, polypeptide 8 )

HSP 1 Score: 407.5 bits (1046), Expect = 1.6e-113
Identity = 213/521 (40.88%), Postives = 324/521 (62.19%), Query Frame = 0

Query: 17  VPVLCAVVVILCAKMAV-----ALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMML 76
           V V  AVVV+    + +       W +P++ E +  RQG+ G P+ F +G+ K   SM+ 
Sbjct: 9   VAVAAAVVVVTTVTVWIWKGLNVAWLRPKKNEAYLKRQGLSGTPFTFLVGDIKREASMVE 68

Query: 77  AASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSE 136
              S P+ N + +   RV+       K +G T  +W GP+  V V+ P+ I+++L    +
Sbjct: 69  QEKSRPI-NLTDDYTHRVMPLIQQTVKDHGKTSYMWMGPIASVIVTKPEHIKDVLNRVYD 128

Query: 137 FYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEK 196
           F  K   HP+V +L   G+   + +KW+ HRKII+P+FH+E LK++IP   ES  +M+ K
Sbjct: 129 F-PKPPVHPIV-ELFATGVALYEGEKWSKHRKIINPSFHLEKLKIMIPAFYESCSEMISK 188

Query: 197 WSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQ 256
           W     +  S   EI+V+     LT DVI++TAFGSSYE+GK IF+LQ +Q     +A +
Sbjct: 189 WEKLVTEQGSSN-EIDVWPYLGDLTSDVISRTAFGSSYEEGKRIFELQEEQGRRVLKALE 248

Query: 257 KVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQ 316
             FIPG RFLPT+ N+   +++KE+K  L ++I  R    ++     E    DLLG++++
Sbjct: 249 LAFIPGMRFLPTKNNLRMRQINKEVKSRLREIIMKR----QRGMDTGEAPKNDLLGILLE 308

Query: 317 ASNSSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVL 376
            SNS      ++ +++ D+VEEC+ F FAG++TT+ LL WT ++L+ H +WQ QAR+E+L
Sbjct: 309 -SNSG-----DHGMSIEDVVEECRLFHFAGQETTAVLLVWTMIMLSHHQKWQDQAREEIL 368

Query: 377 SVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRM-IPRGTEFL 436
            V G    P+ D +S+LK +SMILNE LRLYPP +   R  +++ +LG  M +P G + +
Sbjct: 369 KVIGKNNKPNFDALSRLKTMSMILNEVLRLYPPGILLGRTVEKETKLGEDMTLPGGAQVV 428

Query: 437 IPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAK 496
           IP+L VH D  +WG D  EFNP RF++G+ +A  +   F+PFG G R C GQN AL++AK
Sbjct: 429 IPVLMVHRDPELWGEDVHEFNPERFADGISKATKNQVSFLPFGWGPRFCPGQNFALMEAK 488

Query: 497 LAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 532
           +A+ +ILQRFSF L+P+Y HAP  ++ L+PQ+GAP+IF  +
Sbjct: 489 MALVLILQRFSFELSPSYTHAPHTVLTLHPQFGAPLIFHML 515

BLAST of Lcy03g013350 vs. TAIR 10
Match: AT3G14610.1 (cytochrome P450, family 72, subfamily A, polypeptide 7 )

HSP 1 Score: 402.1 bits (1032), Expect = 6.9e-112
Identity = 208/515 (40.39%), Postives = 317/515 (61.55%), Query Frame = 0

Query: 17  VPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSH 76
           +PVL AVVV+   ++   +W KP+ +E    RQG+ G PY   +G+ K  V MM+ A S 
Sbjct: 9   LPVLVAVVVLWTWRIVKWVWIKPKMLESSLKRQGLTGTPYTPLVGDIKRNVDMMMEARSK 68

Query: 77  PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSEFYEKN 136
           P+ N + +I PR+L         +G TF IW GP+  + +++P+ I+E+    ++F EK 
Sbjct: 69  PI-NVTDDITPRLLPLALKMLNSHGKTFFIWIGPLPTIVITNPEQIKEVFNKVNDF-EKA 128

Query: 137 EAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSATA 196
              PL++ L G GL + K  KWA HR+II+P FH+E +K +IP       ++V +W    
Sbjct: 129 STFPLIRLLAG-GLASYKGDKWASHRRIINPAFHLEKIKNMIPAFYHCCSEVVCQWEKLF 188

Query: 197 DDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFIP 256
            D  S  +E++V+     +T DVI+ TAFGSSY++G+ IF+LQ +   L ++AF+K +IP
Sbjct: 189 TDKES-PLEVDVWPWLVNMTADVISHTAFGSSYKEGQRIFQLQGELAELIAQAFKKSYIP 248

Query: 257 GYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQASNSS 316
           G RF PT++N     +D+E+   L  ++  R    EK     E    DLLG++++   S+
Sbjct: 249 GSRFYPTKSNRRMKAIDREVDVILRGIVSKR----EKAREAGEPANDDLLGILLE---SN 308

Query: 317 SCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSVCGP 376
           S  S  N ++V D+++ECK F+FAG++TTS LL WT VLL+ H  WQ +AR+EV+ V G 
Sbjct: 309 SEESQGNGMSVEDVMKECKLFYFAGQETTSVLLVWTMVLLSHHQDWQARAREEVMQVLGE 368

Query: 377 RALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGTEFLIPILAV 436
              P  + ++ LK+++MI NE LRLYPP     R   ++++LG   +P G +  +P + V
Sbjct: 369 NNKPDMESLNNLKVMTMIFNEVLRLYPPVAQLKRVVNKEMKLGELTLPAGIQIYLPTILV 428

Query: 437 HHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAMTII 496
             D  +WG+DA++F P RF +G+ +A  +   F PFG G R CIGQN A+L+AK+AM +I
Sbjct: 429 QRDTELWGDDAADFKPERFRDGLSKATKNQVSFFPFGWGPRICIGQNFAMLEAKMAMALI 488

Query: 497 LQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 532
           LQ+FSF L+P+Y HAP  +M   PQ+GA +I  K+
Sbjct: 489 LQKFSFELSPSYVHAPQTVMTTRPQFGAHLILHKL 512

BLAST of Lcy03g013350 vs. TAIR 10
Match: AT1G75130.1 (cytochrome P450, family 721, subfamily A, polypeptide 1 )

HSP 1 Score: 397.1 bits (1019), Expect = 2.2e-110
Identity = 213/520 (40.96%), Postives = 313/520 (60.19%), Query Frame = 0

Query: 19  VLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSHPM 78
           +L  V   L  +   +  W P RI+ HF +Q + GP YR F GN  E+  +   A S P+
Sbjct: 6   ILVLVFFFLVFRFIYSNIWVPWRIQSHFKKQSVTGPSYRIFSGNSGEVSRLTAEAKSKPI 65

Query: 79  PN--FSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSEFYEKN 138
           P+    H  + RV   YH W ++YG TFL WFG    VA SDP +IRE LTT   F ++ 
Sbjct: 66  PSGRNPHEFVHRVAPHYHEWSRVYGKTFLYWFGSKPVVATSDPRLIREALTTGGSF-DRI 125

Query: 139 EAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSATA 198
             +PL K L   GL  L+  +WA HR+I    F ME LK  +P +  S + ++EKW    
Sbjct: 126 GHNPLSKLLYAQGLPGLRGDQWAFHRRIAKQAFTMEKLKRWVPQMVTSTMMLMEKW---- 185

Query: 199 DDTASG--EVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVF 258
           +D  +G  E+E+EV      L+ +++++TAFG+S E+GK IF+LQ + M L       V+
Sbjct: 186 EDMRNGGEEIELEVHKEMHNLSAEMLSRTAFGNSVEEGKGIFELQERMMRLFYLVRWSVY 245

Query: 259 IPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQA-- 318
           IPG+RF P++TN   W+++K+I+ S++KLIE  K   EK+            G ++QA  
Sbjct: 246 IPGFRFFPSKTNREIWRIEKQIRVSILKLIENNKTAVEKS------------GTLLQAFM 305

Query: 319 SNSSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLS 378
           S  ++ +     + + ++ +ECK+F+FA K+TT+NL+T+  VLLAM+ +WQ  AR+EV+ 
Sbjct: 306 SPYTNQNGQEEKLGIEEVTDECKTFYFAAKETTANLMTFVLVLLAMNQEWQNIAREEVIC 365

Query: 379 VCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGTEFLIP 438
           V G   LP+ D +  LK LSMI+NE+LRLYPP +   R   +  +LG   IP GT+  + 
Sbjct: 366 VLGQTGLPTLDILQDLKTLSMIINETLRLYPPAMTLNRDTLKRAKLGDLDIPAGTQLYLS 425

Query: 439 ILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLA 498
           ++A+HHD+  WG+DA EFNP RF +         A  +PFGLG RTC+GQNLA+ +AK  
Sbjct: 426 VVAMHHDKETWGDDAEEFNPRRFED----PKKQSALLVPFGLGPRTCVGQNLAVNEAKTV 485

Query: 499 MTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIS 533
           +  IL+ +SFRL+P+Y HAP + + L PQ GA ++F +IS
Sbjct: 486 LATILKYYSFRLSPSYAHAPVLFVTLQPQNGAHLLFTRIS 504

BLAST of Lcy03g013350 vs. TAIR 10
Match: AT3G14680.1 (cytochrome P450, family 72, subfamily A, polypeptide 14 )

HSP 1 Score: 396.7 bits (1018), Expect = 2.9e-110
Identity = 206/497 (41.45%), Postives = 307/497 (61.77%), Query Frame = 0

Query: 35  LWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSHPMPNFSHNILPRVLSFYH 94
           +W+ P+ +E    RQG+ G  Y   IG+FK+++SM + A+S P+   + +I PRV+    
Sbjct: 28  VWFTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFIEATSKPIKP-TDDITPRVMPHPL 87

Query: 95  HWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSEFYEKNEAHPLVKQLEGDGLLTLK 154
              K +G T L WFGP+  + + DP+ I+E+     +F +K    PL K L G GL++  
Sbjct: 88  QMLKTHGRTNLTWFGPIPTITIMDPEQIKEVFNKVYDF-QKAHTFPLSKIL-GTGLVSYD 147

Query: 155 DQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSATADDTASGEVEIEVFDCFQT 214
             KWA HR+II+P FH+E +K ++ V  ES  ++V +W     D  S   E++V+    +
Sbjct: 148 GDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDKLVSDKGS-SCEVDVWPGLTS 207

Query: 215 LTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFIPGYRFLPTRTNISSWKLDK 274
           +T DVI++TAFGSSY +G  IF+LQ +   L  +AFQK FIPGY +LPT+ N       +
Sbjct: 208 MTADVISRTAFGSSYREGHRIFELQAELAQLVMQAFQKFFIPGYIYLPTKGNRRMKTAAR 267

Query: 275 EIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQASNSSSCSSLNNNITVHDIVEEC 334
           EI+  L  +I  R+   E   A  E    DLLG++++   S+   +  N ++  D++EEC
Sbjct: 268 EIQDILRGIINKRERARESGEAPSE----DLLGILLE---SNLGQTEGNGMSTEDMMEEC 327

Query: 335 KSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSVCGPRALPSRDDVSKLKILSMI 394
           K F+ AG++TTS LL WT VLL+ H  WQ +AR+EV  V G +  P  + +++LK+++MI
Sbjct: 328 KLFYLAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKQ-PDTEGLNQLKVMTMI 387

Query: 395 LNESLRLYPPTVATIRRAKEDVELGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGR 454
           L E LRLYPP V   R   ++++LG   +P G +  +P+L VH D  +WGNDA EF P R
Sbjct: 388 LYEVLRLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELWGNDAGEFKPER 447

Query: 455 FSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAMTIILQRFSFRLAPTYQHAPTV 514
           F +G+ +A  +   F PF  G R CIGQN  LL+AK+AM++ILQRFSF L+P+Y HAP  
Sbjct: 448 FKDGLSKATKNQVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSFELSPSYVHAPYT 507

Query: 515 LMLLYPQYGAPIIFQKI 532
           ++ LYPQ+GA ++  K+
Sbjct: 508 IITLYPQFGAHLMLHKL 512