Homology
BLAST of Lcy03g013350.1 vs. ExPASy Swiss-Prot
Match:
O48786 (Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1)
HSP 1 Score: 754.2 bits (1946), Expect = 1.0e-216
Identity = 370/519 (71.29%), Postives = 433/519 (83.43%), Query Frame = 0
Query: 16 KVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASS 75
KV VL ++ ++ K LWW+PR+IEEHF +QGIRGPPY FFIGN KELV MML ASS
Sbjct: 12 KVLVLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASS 71
Query: 76 HPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSEFYEK 135
HPMP FSHNILPRVLSFYHHW+KIYG+TFL+WFGP R+ V+DPD+IREI + SEFYEK
Sbjct: 72 HPMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIF-SKSEFYEK 131
Query: 136 NEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSAT 195
NEAHPLVKQLEGDGLL+LK +KWA HRKIISPTFHMENLKLL+PVV +SV DMV+KWS
Sbjct: 132 NEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDK 191
Query: 196 ADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFI 255
+ +GEVE++V++ FQ LTEDVI++TAFGSSYEDG+A+F+LQ QQM+L +EAFQKVFI
Sbjct: 192 LSE--NGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFI 251
Query: 256 PGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGP--KDLLGLMIQAS 315
PGYRF PTR N+ SWKLDKEI+KSL+KLIE R+ N+ + K P KDLLGLMIQA
Sbjct: 252 PGYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQA- 311
Query: 316 NSSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSV 375
N+TV DIVEECKSFFFAGKQTTSNLLTWTT+LL+MHP+WQ +ARDEVL V
Sbjct: 312 ---------KNVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRV 371
Query: 376 CGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGTEFLIPI 435
CG R +P++D V KLK LSMILNESLRLYPP VATIRRAK DV+LGG IP GTE LIPI
Sbjct: 372 CGSRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPI 431
Query: 436 LAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAM 495
+AVHHDQ+IWGND +EFNP RF++GV RA HP GFIPFGLGVRTCIGQNLA+LQAKL +
Sbjct: 432 IAVHHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTL 491
Query: 496 TIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIS 533
+++QRF+F LAPTYQHAPTVLMLLYPQ+GAPI F++++
Sbjct: 492 AVMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRLT 516
BLAST of Lcy03g013350.1 vs. ExPASy Swiss-Prot
Match:
B9X287 (Cytochrome P450 734A6 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A6 PE=2 SV=1)
HSP 1 Score: 660.2 bits (1702), Expect = 2.0e-188
Identity = 321/515 (62.33%), Postives = 405/515 (78.64%), Query Frame = 0
Query: 22 AVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSHPM--P 81
A + K+ LWW+PRR+EEHF RQGI GP YRF +G +E+V++M+AAS+ PM P
Sbjct: 12 AAAAWVAVKVLEVLWWRPRRVEEHFARQGITGPRYRFLVGCVREMVALMVAASAKPMPPP 71
Query: 82 NFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSEFYEKNEAH 141
SHN+LPRVL+FYHHWKKIYGSTFLIWFGP R+A++DP++IRE+L ++ +++ E+H
Sbjct: 72 YRSHNVLPRVLAFYHHWKKIYGSTFLIWFGPTPRLAIADPELIREVLLARADRFDRYESH 131
Query: 142 PLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSATADDT 201
P+V+QLEG+GL++L+ KWA HR++++P FHM+NL+LL+P V +V+DM +KW A A+
Sbjct: 132 PMVRQLEGEGLVSLRGDKWAHHRRVLTPAFHMDNLRLLLPCVGMTVLDMADKWRAMAEAD 191
Query: 202 ASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFIPGYR 261
SGEVEI+V D FQ +TED IT+TAFG SYEDGK +FKLQ Q M ASEAF+KVFIPGYR
Sbjct: 192 KSGEVEIDVSDWFQVVTEDAITRTAFGRSYEDGKVVFKLQAQLMAFASEAFRKVFIPGYR 251
Query: 262 FLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQASNSSSCS 321
FLPT+ N SSWKLDKEI+K+L+ LI GR+ + + +D KDLLGLMI A+ SS+
Sbjct: 252 FLPTKKNTSSWKLDKEIRKNLVTLI-GRRQEAGDDEKLDGCA-KDLLGLMINAAASSNGG 311
Query: 322 SLN----NNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSVCG 381
+ + ITV+DIVEECK+FFFAGKQTTSNLLTW V+LAMHP+WQ +AR EVL VCG
Sbjct: 312 KRSALPVSPITVNDIVEECKTFFFAGKQTTSNLLTWAIVVLAMHPEWQERARQEVLDVCG 371
Query: 382 PRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRM-IPRGTEFLIPIL 441
+PSR+ ++KLK L MILNE+LRLYPP VAT+RRAK DVELGG + IPR TE LIPI+
Sbjct: 372 ADGVPSREQLAKLKTLGMILNETLRLYPPAVATVRRAKADVELGGYLRIPRDTELLIPIM 431
Query: 442 AVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAMT 501
AVHHD +WG DA++FNP RF+ GV RA HPA FIPFGLG R CIGQNLA+L+AKL +
Sbjct: 432 AVHHDARLWGPDAAQFNPARFAGGVARAARHPAAFIPFGLGARMCIGQNLAILEAKLTVA 491
Query: 502 IILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQ 530
+IL RF FRL+ Y HAPTVLMLL+PQYGAPI+F+
Sbjct: 492 VILHRFEFRLSARYVHAPTVLMLLHPQYGAPIVFR 524
BLAST of Lcy03g013350.1 vs. ExPASy Swiss-Prot
Match:
Q6Z6D6 (Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 SV=1)
HSP 1 Score: 617.8 bits (1592), Expect = 1.1e-175
Identity = 298/529 (56.33%), Postives = 388/529 (73.35%), Query Frame = 0
Query: 23 VVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSHPM-PNF 82
V + + A+MA ALWW+PRR+E HF QG+RGPPYRF +G+ +E+V++M ASS PM P
Sbjct: 29 VTMHVAARMADALWWRPRRLEAHFAAQGVRGPPYRFLLGSVREMVALMAEASSKPMSPPT 88
Query: 83 SHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSEFYEKNEAHPL 142
SHN LPRVL+FYH+W+KIYG FLIWFGP R+ V++P++IREI T ++ +++ EAHP+
Sbjct: 89 SHNALPRVLAFYHYWRKIYGHRFLIWFGPTPRLTVAEPELIREIFLTRADAFDRYEAHPV 148
Query: 143 VKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSATADDTAS 202
V+QLEGDGL++L KWALHR++++ F+ +NL LIP V +SV + KW A A+ S
Sbjct: 149 VRQLEGDGLVSLHGDKWALHRRVLTDAFYPDNLNRLIPHVGKSVAALAAKWGAMAEAGGS 208
Query: 203 GEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFIPGYRFL 262
GEVE++V + FQ +TE+ IT+ FG SY+DG+ +F +Q Q M ASEAF+KV +PGYRFL
Sbjct: 209 GEVEVDVAEWFQAVTEEAITRATFGRSYDDGRVVFAMQGQLMAFASEAFRKVLVPGYRFL 268
Query: 263 PTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQASNSSSC--- 322
PT+ N SW+LD+EI++SLM+LI R +E+ D+ +DLLGLMI A +++
Sbjct: 269 PTKKNRLSWRLDREIRRSLMRLIGRRSDEAEQGEKADDGSFRDLLGLMINAGAAAATRGN 328
Query: 323 -----SSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSV 382
+S I V D++EECK+FFFAGKQTT+NLLTW TVLLAMHP WQ +AR EV V
Sbjct: 329 AGGEKNSPAAAIPVEDMLEECKTFFFAGKQTTTNLLTWATVLLAMHPDWQERARREVFDV 388
Query: 383 CGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELG-GRMIPRGTEFLIP 442
CG LPS++ + KLK L MI+NE+LRLYPP VATIRRAK DV+L G MIPR E L+P
Sbjct: 389 CGAGELPSKEHLPKLKTLGMIMNETLRLYPPAVATIRRAKVDVQLSDGCMIPRDMELLVP 448
Query: 443 ILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLA 502
I+A+HHD WG DAS+FNP RF+ G +A HP FIPFGLG R C+GQNLA L+AKL
Sbjct: 449 IMAIHHDTRYWGPDASQFNPARFANGASKAAKHPLAFIPFGLGSRMCVGQNLARLEAKLT 508
Query: 503 MTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKISQFTGDASMP 542
M I+LQRF R +P Y HAPTVLMLLYPQYGAP+IF+ +S D++ P
Sbjct: 509 MAILLQRFEIRTSPNYVHAPTVLMLLYPQYGAPLIFRPLSSHPPDSTGP 557
BLAST of Lcy03g013350.1 vs. ExPASy Swiss-Prot
Match:
Q69XM6 (Cytochrome P450 734A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A4 PE=2 SV=1)
HSP 1 Score: 592.0 bits (1525), Expect = 6.6e-168
Identity = 292/537 (54.38%), Postives = 386/537 (71.88%), Query Frame = 0
Query: 12 LESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMML 71
+E+V V +++ + A++A A+WW+PRR+E HF QG+RGPPYRF +G +E+V++M
Sbjct: 2 MEAVAVAAAVLLLLHVAARVADAVWWRPRRLEAHFAGQGVRGPPYRFLVGCVREMVALMA 61
Query: 72 AASSHPMPNFS-HNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNS 131
A++ PMP + HN LPRVL+FYH+W+KIYG TFLIWFGP R+ V++P+M+REI T +
Sbjct: 62 EATAKPMPPAAPHNALPRVLAFYHYWRKIYGPTFLIWFGPTPRLTVAEPEMVREIFLTRA 121
Query: 132 EFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVE 191
E +++ EAHP+V+QLEGDGL++L KWA HR++++P F+ +NL L+P V SV + E
Sbjct: 122 EAFDRYEAHPVVRQLEGDGLVSLHGDKWAHHRRVLTPGFYPDNLNRLVPHVGRSVAALAE 181
Query: 192 KWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAF 251
+W A A GEVE++V + FQ + E+ IT+ FG SY+ G+ +F+LQ + M ASEAF
Sbjct: 182 RWRAMA-CAGGGEVEVDVAEWFQAVAEEAITRATFGRSYDSGRVVFRLQARLMAFASEAF 241
Query: 252 QKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKG-----NSEKNWAMDEK---GP 311
+KV +PGYRFLPT+ N SW LD+EI++ L++LI R G E + +K G
Sbjct: 242 RKVLVPGYRFLPTKKNRMSWGLDREIRRGLVRLIGRRSGGDGGEEDETTTELKDKQDSGF 301
Query: 312 KDLLGLMIQASNSSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQW 371
DLLGLMI A ++ + V D+VEECK+FFFAGKQTT+NLLTW TVLLAMHP W
Sbjct: 302 NDLLGLMINA-------GVDRTMPVEDMVEECKTFFFAGKQTTTNLLTWATVLLAMHPDW 361
Query: 372 QIQARDEVLSVCGPRA--LPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGG 431
Q +AR EVL+VCG A LP++D + KLK L MILNE+LRLYPP VATIRRAK DV LGG
Sbjct: 362 QDRARREVLAVCGDAAGELPTKDHLPKLKTLGMILNETLRLYPPAVATIRRAKFDVTLGG 421
Query: 432 R--------MIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPF 491
IPR TE L+PI+A+HHD +WG DA++FNP RF+ G RA HP FIPF
Sbjct: 422 GGDGDAGGIHIPRDTELLVPIMAIHHDARLWGPDAAQFNPARFASGAARAAKHPLAFIPF 481
Query: 492 GLGVRTCIGQNLALLQAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQ 530
GLG R CIGQ+LA+L+AKL M ++LQRF L+PTY HAPTVLMLL+PQYGAP+IF+
Sbjct: 482 GLGSRMCIGQSLAILEAKLTMAVLLQRFDLALSPTYVHAPTVLMLLHPQYGAPLIFR 530
BLAST of Lcy03g013350.1 vs. ExPASy Swiss-Prot
Match:
Q8LIF2 (Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A5 PE=2 SV=1)
HSP 1 Score: 534.6 bits (1376), Expect = 1.3e-150
Identity = 261/512 (50.98%), Postives = 360/512 (70.31%), Query Frame = 0
Query: 24 VVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSHPM-PNFS 83
V A+ A ALWW+PRR+E HF QG+RGP YRFF+G+ ELV +M+ A+S PM P S
Sbjct: 28 VYAAAARAAEALWWRPRRVERHFAAQGVRGPGYRFFVGSSIELVRLMVDAASRPMEPPTS 87
Query: 84 HNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSEFYEKNEAHPLV 143
H+ILPRVL FYHHW+K+YG LIWFG R+ VS+P++IRE+L T ++ +++ EAHP++
Sbjct: 88 HDILPRVLPFYHHWRKLYGPMHLIWFGRTPRLVVSEPELIREVLLTRADHFDRYEAHPMI 147
Query: 144 KQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSATADDTASG 203
Q EG GL L ++WA R++++P FH ENL+++ P V +V M+++ + A +G
Sbjct: 148 CQFEGYGLSNLHGERWARRRRVLTPAFHTENLRMIAPFVAGTVTRMLDELAERARAGGAG 207
Query: 204 EVEIEVFDCFQTLTEDVITKTAFG-SSYEDGKAIFKLQTQQMILASEAFQKVFIPGYRFL 263
E E++V + FQ + ++ IT AFG +Y+DG A+F+LQ + A+EA KV+IPGYRFL
Sbjct: 208 EAEVDVAEWFQRVPQEAITFAAFGRRNYDDGAAVFRLQDELAGYATEAHSKVYIPGYRFL 267
Query: 264 PTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQASNSSSCSSL 323
PTR N W+LD+EI+ L K + G + S + + G G+ S +
Sbjct: 268 PTRKNRRVWQLDREIRSHLAKFVTGLQSCSSSHGDDADDGGDGGGGM------REFMSFM 327
Query: 324 NNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSVCGPRALPS 383
+T +I+EE K+FFFAGK+T SNLLTWTTV LAMHP+WQ +AR EV++VCG LP+
Sbjct: 328 APAMTAGEIIEESKNFFFAGKETLSNLLTWTTVALAMHPEWQERARREVVAVCGRGDLPT 387
Query: 384 RDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGTEFLIPILAVHHDQS 443
+D + KLK L MILNE+LRLYPP VA IR AKEDVELGG ++P GTE +IPI+AVHHD +
Sbjct: 388 KDHLPKLKTLGMILNETLRLYPPAVAMIRTAKEDVELGGCVVPAGTEVMIPIMAVHHDAA 447
Query: 444 IWGNDASEFNPGRF-SEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAMTIILQRF 503
WG+DA+EFNP RF ++ G HP F+PFG G R CIGQN+AL++AK+A+ ++L+RF
Sbjct: 448 AWGDDAAEFNPARFAADDDGGRRRHPMAFMPFGGGARVCIGQNMALMEAKVALAVVLRRF 507
Query: 504 SFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIS 533
FRL+P Y HAP VLM+L PQ+GAP+IF+ ++
Sbjct: 508 EFRLSPAYVHAPRVLMILSPQFGAPVIFRPLT 533
BLAST of Lcy03g013350.1 vs. ExPASy TrEMBL
Match:
A0A6J1L528 (cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111499208 PE=3 SV=1)
HSP 1 Score: 941.8 bits (2433), Expect = 1.3e-270
Identity = 465/530 (87.74%), Postives = 493/530 (93.02%), Query Frame = 0
Query: 9 PAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVS 68
P P +S+K+P LCA++ IL KMAVALWWKPRRIE HFLRQGIRGPPYRFFIGNFKEL S
Sbjct: 6 PPPFDSIKIPFLCALLFILAVKMAVALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDS 65
Query: 69 MMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTT 128
MMLAA+S PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGP+VRVAVSDPDMIR+ILTT
Sbjct: 66 MMLAATSRPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTT 125
Query: 129 NSEFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDM 188
NSEFYEKNEAHPL+K+LEGDGLLTLKDQKWALHRKIISP F+MENLKLLIPVVEESVVDM
Sbjct: 126 NSEFYEKNEAHPLIKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDM 185
Query: 189 VEKWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASE 248
VEKWSA A AS EVEI+VFD FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILASE
Sbjct: 186 VEKWSAMAGGAASDEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASE 245
Query: 249 AFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGL 308
AFQKVFIPGYRFLPTRTNI+SWKLDKEI+KSLMKLIE RKGNSEK WAMDEK PKDLLGL
Sbjct: 246 AFQKVFIPGYRFLPTRTNINSWKLDKEIRKSLMKLIESRKGNSEKIWAMDEKAPKDLLGL 305
Query: 309 MIQASN-SSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 368
MIQASN SSS SSL+ NI+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR
Sbjct: 306 MIQASNSSSSSSSLHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 365
Query: 369 DEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGT 428
DEVLSVCGPRALPSRDD+SK KILSMILNESLRLYPPTVATIRRAK VEL GRMIPRGT
Sbjct: 366 DEVLSVCGPRALPSRDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMIPRGT 425
Query: 429 EFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALL 488
EFLIPILAVHHDQ+IWGNDASEFNPGRFSEGVG AGNHPAGFIPFG G+R CIG+NLALL
Sbjct: 426 EFLIPILAVHHDQNIWGNDASEFNPGRFSEGVGHAGNHPAGFIPFGHGLRACIGKNLALL 485
Query: 489 QAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKISQFTGD 538
QAKLA+ II+QRFSFRLAP+YQHAP VLMLLYPQYGAP++FQKI + D
Sbjct: 486 QAKLAIAIIVQRFSFRLAPSYQHAPAVLMLLYPQYGAPVVFQKIVETRRD 535
BLAST of Lcy03g013350.1 vs. ExPASy TrEMBL
Match:
A0A6J1EZI1 (cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111440975 PE=3 SV=1)
HSP 1 Score: 937.6 bits (2422), Expect = 2.4e-269
Identity = 462/524 (88.17%), Postives = 491/524 (93.70%), Query Frame = 0
Query: 9 PAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVS 68
P PL+S+K+P LCA++ IL KM ALWWKPRRIE HFLRQGIRGPPYRFFIGNFKEL S
Sbjct: 6 PPPLDSIKIPFLCALLFILAVKMGAALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDS 65
Query: 69 MMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTT 128
MMLAA+S PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGP+VRVAVSDPDMIR+ILTT
Sbjct: 66 MMLAATSRPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTT 125
Query: 129 NSEFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDM 188
NSEFYEKNEAHPL+K+LEGDGLLTLKDQKWALHRKIISP F+MENLKLLIPVVEESVVDM
Sbjct: 126 NSEFYEKNEAHPLIKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDM 185
Query: 189 VEKWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASE 248
VEKWSA A AS EVEI+VFD FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILASE
Sbjct: 186 VEKWSAMAGGGASDEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASE 245
Query: 249 AFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGL 308
AFQKVFIPGYRFLPTRTNI+SWKLDKEI+KSLMKLIEGRKGNS+K WAMDEK PKDLLGL
Sbjct: 246 AFQKVFIPGYRFLPTRTNINSWKLDKEIRKSLMKLIEGRKGNSQKIWAMDEKAPKDLLGL 305
Query: 309 MIQASN-SSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 368
MIQASN SSS SSL+ NI+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR
Sbjct: 306 MIQASNSSSSSSSLHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 365
Query: 369 DEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGT 428
DEVLSVCGPRALPS DD+SK KILSMILNESLRLYPPTVATIRRAK VEL GRMIPRGT
Sbjct: 366 DEVLSVCGPRALPSTDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMIPRGT 425
Query: 429 EFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALL 488
EFLIPILAVHHDQ+IWGNDASEFNPGRFSEGVG AGNHPAGFIPFG G+R CIG+NLALL
Sbjct: 426 EFLIPILAVHHDQNIWGNDASEFNPGRFSEGVGHAGNHPAGFIPFGHGLRACIGKNLALL 485
Query: 489 QAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 532
QAKLA+ II+QRFSFRLAP+YQHAPTVLMLLYPQYGAP++F+KI
Sbjct: 486 QAKLAIAIIVQRFSFRLAPSYQHAPTVLMLLYPQYGAPVVFRKI 529
BLAST of Lcy03g013350.1 vs. ExPASy TrEMBL
Match:
A0A1S3CMW0 (cytochrome P450 734A1-like OS=Cucumis melo OX=3656 GN=LOC103502730 PE=3 SV=1)
HSP 1 Score: 899.8 bits (2324), Expect = 5.5e-258
Identity = 446/537 (83.05%), Postives = 487/537 (90.69%), Query Frame = 0
Query: 1 MKENPAPAPAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFI 60
MKE +PA + V ++ I+ AK+ + LWWKP++IE+HF RQGI+GPPYRFFI
Sbjct: 1 MKEVKIESPA-WSAAAVTTAVLLIFIISAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFI 60
Query: 61 GNFKELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPD 120
GNFKELVS+MLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGP+VRVAVSDPD
Sbjct: 61 GNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPD 120
Query: 121 MIREILTTNSEFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPV 180
MIREI TNS++YEKNEAHPLVKQLEGDGLLTL QKWALHRKIISPTFH++NLKLLIP+
Sbjct: 121 MIREIFATNSQYYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPL 180
Query: 181 VEESVVDMVEKWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQT 240
VEESVV+MVEKW + A+ S EVEI+V+D FQTLTEDVITKTAFGSSYEDGKAIFKLQ
Sbjct: 181 VEESVVEMVEKWWSMAESVESSEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQN 240
Query: 241 QQMILASEAFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSE-KNWAMDE 300
QQMILAS+AFQKVFIPGYRFLPTRTNI+SWKLDKEIKKSLMKLIEGRKGN E KNW M+E
Sbjct: 241 QQMILASQAFQKVFIPGYRFLPTRTNINSWKLDKEIKKSLMKLIEGRKGNLEKKNWGMNE 300
Query: 301 KGPKDLLGLMIQAS-----NSSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTV 360
K PKDLLGLMIQAS N+++ ++ N++ITVHDIVEECKSFFFAGKQTTSNLLTWTTV
Sbjct: 301 KRPKDLLGLMIQASLLNNNNNNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTV 360
Query: 361 LLAMHPQWQIQARDEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKE 420
LLAMHPQWQIQARDEV++VCGPRALPS+ DVS KILSMILNESLRLYPPTVATIRRAK
Sbjct: 361 LLAMHPQWQIQARDEVINVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKA 420
Query: 421 DVELGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGL 480
DVEL GRMIPRGTEFLIPI+AVHHDQ+IWGNDASEFNPGRFSEGV RAGNHPAGFIPFGL
Sbjct: 421 DVELAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSRAGNHPAGFIPFGL 480
Query: 481 GVRTCIGQNLALLQAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 532
GVRTCIGQNLA+L+AKL M IILQRF+FRLAPTYQHAPTVLMLLYPQYGAPIIFQKI
Sbjct: 481 GVRTCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 536
BLAST of Lcy03g013350.1 vs. ExPASy TrEMBL
Match:
A0A0A0LU20 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G305710 PE=3 SV=1)
HSP 1 Score: 897.5 bits (2318), Expect = 2.7e-257
Identity = 445/532 (83.65%), Postives = 486/532 (91.35%), Query Frame = 0
Query: 1 MKENPAPAPAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFI 60
MKE +PA +V V ++ ++ I+ AK+ + LWWKP++IE+HF RQGI+GPPYRFFI
Sbjct: 1 MKEVKIESPAWSATV-VTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFI 60
Query: 61 GNFKELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPD 120
GNFKELVS+MLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGP+VRVAVSDPD
Sbjct: 61 GNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPD 120
Query: 121 MIREILTTNSEFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPV 180
MIREI TNS FYEKNEAHPLVKQLEGDGLLTL QKWALHRKIISPTFH++NLKLLIP+
Sbjct: 121 MIREIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPL 180
Query: 181 VEESVVDMVEKWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQT 240
VEESVV+MVEKW + A+ SGEVEI+V+D FQTLTEDVITKTAFGSSYEDGKAIFKLQT
Sbjct: 181 VEESVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQT 240
Query: 241 QQMILASEAFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSE-KNWAMDE 300
QQMILAS+AFQKVFIPGYRFLPTRTNI+SWKLD+EIKKSLMKLIEGRK N E KNW M+E
Sbjct: 241 QQMILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGMNE 300
Query: 301 KGPKDLLGLMIQASNSSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMH 360
K PKDLLGLMIQAS ++ ++ N++ITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMH
Sbjct: 301 KRPKDLLGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMH 360
Query: 361 PQWQIQARDEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELG 420
PQWQIQARDEV +VCGPRALPS+ DVS KILSMILNESLRLYPPTVATIRRAK DVEL
Sbjct: 361 PQWQIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVELA 420
Query: 421 GRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTC 480
GRMIPRGTEFLIPI+AVHHDQ+IWGNDASEFNPGRFSEGV +A NHPAGFIPFGLGVRTC
Sbjct: 421 GRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVRTC 480
Query: 481 IGQNLALLQAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 532
IGQNLALL+AKL + IILQRF+FRLAPTYQHAPTVLMLLYPQYGAPIIFQKI
Sbjct: 481 IGQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 531
BLAST of Lcy03g013350.1 vs. ExPASy TrEMBL
Match:
A0A6J1H9G1 (cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111461725 PE=3 SV=1)
HSP 1 Score: 887.5 bits (2292), Expect = 2.8e-254
Identity = 436/516 (84.50%), Postives = 465/516 (90.12%), Query Frame = 0
Query: 17 VPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSH 76
V VVVIL AKM + LWWKPRRIE HFLRQGI GPPYRFFIGNFKELVSMM AASSH
Sbjct: 15 VAAAAVVVVILGAKMGLVLWWKPRRIENHFLRQGIGGPPYRFFIGNFKELVSMMAAASSH 74
Query: 77 PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSEFYEKN 136
P+PNFSHNILPRVLSFYHHWKKIYGSTF+IWFGP+ RVAVSDPDMIREI TNS FYEKN
Sbjct: 75 PIPNFSHNILPRVLSFYHHWKKIYGSTFVIWFGPIARVAVSDPDMIREIFITNSHFYEKN 134
Query: 137 EAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSATA 196
EAHPLVKQLEGDGLLTL KWALHRKIISPTFH +NLKLLIP+VEESV+DMV+KWS+
Sbjct: 135 EAHPLVKQLEGDGLLTLNGPKWALHRKIISPTFHTDNLKLLIPLVEESVMDMVQKWSSMT 194
Query: 197 DDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFIP 256
++T SGEVEI+VFD FQTLTEDVITKTAFG SYEDGKAIFK+QTQQMILASEAFQKVFIP
Sbjct: 195 EETTSGEVEIDVFDSFQTLTEDVITKTAFGCSYEDGKAIFKIQTQQMILASEAFQKVFIP 254
Query: 257 GYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQASNSS 316
GYRFLPTRTN+ SWKL+KEIKKSL+KLIEGRK N EKNWA++EKGPKDLLGLMIQAS
Sbjct: 255 GYRFLPTRTNMKSWKLEKEIKKSLLKLIEGRKRNFEKNWAINEKGPKDLLGLMIQAS--- 314
Query: 317 SCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSVCGP 376
I+VHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHP+WQI AR EV ++CGP
Sbjct: 315 --------ISVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPRWQILARHEVFTICGP 374
Query: 377 RALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGTEFLIPILAV 436
RALPS DDVSK KILSMILNESLRLYPPTVATIRRA DVELGGRMIPRGTEFLIPILAV
Sbjct: 375 RALPSSDDVSKFKILSMILNESLRLYPPTVATIRRANADVELGGRMIPRGTEFLIPILAV 434
Query: 437 HHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAMTII 496
HHDQ+IWGNDASEFNP RFS+GV RAGNHP GFIPFGLGVR CIGQNLA+L+AKLAM II
Sbjct: 435 HHDQTIWGNDASEFNPERFSDGVSRAGNHPVGFIPFGLGVRNCIGQNLAVLEAKLAMVII 494
Query: 497 LQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIS 533
LQR SFRLAPTYQHAPTVLMLLYPQYGAPI+F+KI+
Sbjct: 495 LQRLSFRLAPTYQHAPTVLMLLYPQYGAPILFEKIA 519
BLAST of Lcy03g013350.1 vs. NCBI nr
Match:
XP_023531517.1 (cytochrome P450 734A1-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 942.6 bits (2435), Expect = 1.5e-270
Identity = 466/528 (88.26%), Postives = 494/528 (93.56%), Query Frame = 0
Query: 9 PAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVS 68
P PL+S+K+P LCA++ IL KMAVALWWKPRRIE HFLRQGIRGPPYRFFIGNFKEL S
Sbjct: 6 PPPLDSIKIPFLCALLFILAVKMAVALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDS 65
Query: 69 MMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTT 128
MMLAA+S PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGP+VRVAVSDPDMIR+ILTT
Sbjct: 66 MMLAATSRPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTT 125
Query: 129 NSEFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDM 188
NSEFYEKNEAHPL+K+LEGDGLLTLKDQKWALHRKIISP F+MENLKLLIPVVEESVVDM
Sbjct: 126 NSEFYEKNEAHPLIKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDM 185
Query: 189 VEKWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASE 248
VEKWSA A AS EVEI+VFD FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILASE
Sbjct: 186 VEKWSAMAGGAASDEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASE 245
Query: 249 AFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGL 308
AFQKVFIPGYRFLPTRTNI+SWKLDKEI+KSLMKLIEGRKGNS+K WAMDEK PKDLLGL
Sbjct: 246 AFQKVFIPGYRFLPTRTNINSWKLDKEIRKSLMKLIEGRKGNSQKIWAMDEKAPKDLLGL 305
Query: 309 MIQASN-----SSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQ 368
MIQASN SSS SSL+ NI+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQ
Sbjct: 306 MIQASNSSSSSSSSSSSLHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQ 365
Query: 369 IQARDEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMI 428
IQARDEVLSVCGPRALPSRDD+SK KILSMILNESLRLYPPTVATIRRAK VEL GRMI
Sbjct: 366 IQARDEVLSVCGPRALPSRDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMI 425
Query: 429 PRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQN 488
PRGTEFLIPILAVHHDQ+IWGNDASEFNPGRFSEGVG AGNHPAGFIPFG G+R CIG+N
Sbjct: 426 PRGTEFLIPILAVHHDQNIWGNDASEFNPGRFSEGVGHAGNHPAGFIPFGHGLRACIGKN 485
Query: 489 LALLQAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 532
LALLQAKLA+ II+QRFSFRLAP+YQHAPTVLMLLYPQYGAPI+F+KI
Sbjct: 486 LALLQAKLAIAIIVQRFSFRLAPSYQHAPTVLMLLYPQYGAPIVFRKI 533
BLAST of Lcy03g013350.1 vs. NCBI nr
Match:
XP_023006493.1 (cytochrome P450 734A1-like [Cucurbita maxima])
HSP 1 Score: 941.8 bits (2433), Expect = 2.6e-270
Identity = 465/530 (87.74%), Postives = 493/530 (93.02%), Query Frame = 0
Query: 9 PAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVS 68
P P +S+K+P LCA++ IL KMAVALWWKPRRIE HFLRQGIRGPPYRFFIGNFKEL S
Sbjct: 6 PPPFDSIKIPFLCALLFILAVKMAVALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDS 65
Query: 69 MMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTT 128
MMLAA+S PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGP+VRVAVSDPDMIR+ILTT
Sbjct: 66 MMLAATSRPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTT 125
Query: 129 NSEFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDM 188
NSEFYEKNEAHPL+K+LEGDGLLTLKDQKWALHRKIISP F+MENLKLLIPVVEESVVDM
Sbjct: 126 NSEFYEKNEAHPLIKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDM 185
Query: 189 VEKWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASE 248
VEKWSA A AS EVEI+VFD FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILASE
Sbjct: 186 VEKWSAMAGGAASDEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASE 245
Query: 249 AFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGL 308
AFQKVFIPGYRFLPTRTNI+SWKLDKEI+KSLMKLIE RKGNSEK WAMDEK PKDLLGL
Sbjct: 246 AFQKVFIPGYRFLPTRTNINSWKLDKEIRKSLMKLIESRKGNSEKIWAMDEKAPKDLLGL 305
Query: 309 MIQASN-SSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 368
MIQASN SSS SSL+ NI+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR
Sbjct: 306 MIQASNSSSSSSSLHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 365
Query: 369 DEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGT 428
DEVLSVCGPRALPSRDD+SK KILSMILNESLRLYPPTVATIRRAK VEL GRMIPRGT
Sbjct: 366 DEVLSVCGPRALPSRDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMIPRGT 425
Query: 429 EFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALL 488
EFLIPILAVHHDQ+IWGNDASEFNPGRFSEGVG AGNHPAGFIPFG G+R CIG+NLALL
Sbjct: 426 EFLIPILAVHHDQNIWGNDASEFNPGRFSEGVGHAGNHPAGFIPFGHGLRACIGKNLALL 485
Query: 489 QAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKISQFTGD 538
QAKLA+ II+QRFSFRLAP+YQHAP VLMLLYPQYGAP++FQKI + D
Sbjct: 486 QAKLAIAIIVQRFSFRLAPSYQHAPAVLMLLYPQYGAPVVFQKIVETRRD 535
BLAST of Lcy03g013350.1 vs. NCBI nr
Match:
KAG7021795.1 (Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 937.6 bits (2422), Expect = 4.9e-269
Identity = 462/527 (87.67%), Postives = 493/527 (93.55%), Query Frame = 0
Query: 9 PAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVS 68
P PL+S+K+P LCA++ IL KM VALWWKPRRIE HFLRQGIRGPPYRFFIGNFKEL S
Sbjct: 6 PPPLDSIKIPFLCALLFILAVKMGVALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDS 65
Query: 69 MMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTT 128
MMLAA+S PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGP+VRVAVSDPDMIR+ILT
Sbjct: 66 MMLAATSRPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTN 125
Query: 129 NSEFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDM 188
NSEFYEKNEAHPL+K+LEGDGLLTLKDQKWALHRKIISP F+MENLKLLIPVVEESVVDM
Sbjct: 126 NSEFYEKNEAHPLIKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDM 185
Query: 189 VEKWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASE 248
VEKWSA A AS EVEI+VFD FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILASE
Sbjct: 186 VEKWSAMAGGGASDEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASE 245
Query: 249 AFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGL 308
AFQKVFIPGYRFLPTRTNI+SWKLDKEI+KSLMKLIEGRKGNS+K WAMDEK PKDLLGL
Sbjct: 246 AFQKVFIPGYRFLPTRTNINSWKLDKEIRKSLMKLIEGRKGNSQKIWAMDEKAPKDLLGL 305
Query: 309 MIQASN----SSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQI 368
MIQASN SSS SSL+ NI+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQI
Sbjct: 306 MIQASNSSSSSSSSSSLHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQI 365
Query: 369 QARDEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIP 428
QARDEVLS+CGPRALPSRDD+SK KILSMILNESLRLYPPTVATIRRAK VEL GRMIP
Sbjct: 366 QARDEVLSLCGPRALPSRDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMIP 425
Query: 429 RGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNL 488
RGTEFLIPILAVHHDQ+IWGNDASEFNPGRFSEGVG+AGNHPAGFIPFG G+R CIG+NL
Sbjct: 426 RGTEFLIPILAVHHDQNIWGNDASEFNPGRFSEGVGQAGNHPAGFIPFGHGLRACIGKNL 485
Query: 489 ALLQAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 532
ALLQAKLA+ II+QRFSFRLAP+YQHAPTVLMLLYPQYGAP++F+KI
Sbjct: 486 ALLQAKLAIAIIVQRFSFRLAPSYQHAPTVLMLLYPQYGAPVVFRKI 532
BLAST of Lcy03g013350.1 vs. NCBI nr
Match:
XP_022933596.1 (cytochrome P450 734A1-like [Cucurbita moschata])
HSP 1 Score: 937.6 bits (2422), Expect = 4.9e-269
Identity = 462/524 (88.17%), Postives = 491/524 (93.70%), Query Frame = 0
Query: 9 PAPLESVKVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVS 68
P PL+S+K+P LCA++ IL KM ALWWKPRRIE HFLRQGIRGPPYRFFIGNFKEL S
Sbjct: 6 PPPLDSIKIPFLCALLFILAVKMGAALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDS 65
Query: 69 MMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTT 128
MMLAA+S PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGP+VRVAVSDPDMIR+ILTT
Sbjct: 66 MMLAATSRPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTT 125
Query: 129 NSEFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDM 188
NSEFYEKNEAHPL+K+LEGDGLLTLKDQKWALHRKIISP F+MENLKLLIPVVEESVVDM
Sbjct: 126 NSEFYEKNEAHPLIKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDM 185
Query: 189 VEKWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASE 248
VEKWSA A AS EVEI+VFD FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILASE
Sbjct: 186 VEKWSAMAGGGASDEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASE 245
Query: 249 AFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGL 308
AFQKVFIPGYRFLPTRTNI+SWKLDKEI+KSLMKLIEGRKGNS+K WAMDEK PKDLLGL
Sbjct: 246 AFQKVFIPGYRFLPTRTNINSWKLDKEIRKSLMKLIEGRKGNSQKIWAMDEKAPKDLLGL 305
Query: 309 MIQASN-SSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 368
MIQASN SSS SSL+ NI+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR
Sbjct: 306 MIQASNSSSSSSSLHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 365
Query: 369 DEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGT 428
DEVLSVCGPRALPS DD+SK KILSMILNESLRLYPPTVATIRRAK VEL GRMIPRGT
Sbjct: 366 DEVLSVCGPRALPSTDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMIPRGT 425
Query: 429 EFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALL 488
EFLIPILAVHHDQ+IWGNDASEFNPGRFSEGVG AGNHPAGFIPFG G+R CIG+NLALL
Sbjct: 426 EFLIPILAVHHDQNIWGNDASEFNPGRFSEGVGHAGNHPAGFIPFGHGLRACIGKNLALL 485
Query: 489 QAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 532
QAKLA+ II+QRFSFRLAP+YQHAPTVLMLLYPQYGAP++F+KI
Sbjct: 486 QAKLAIAIIVQRFSFRLAPSYQHAPTVLMLLYPQYGAPVVFRKI 529
BLAST of Lcy03g013350.1 vs. NCBI nr
Match:
XP_038880221.1 (cytochrome P450 734A1-like [Benincasa hispida])
HSP 1 Score: 931.4 bits (2406), Expect = 3.5e-267
Identity = 466/530 (87.92%), Postives = 490/530 (92.45%), Query Frame = 0
Query: 12 LESV--KVP------VLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNF 71
+ESV KVP V AVV+IL AKM + LWWKPRRIE+HF RQGIRGPPYRFFIGNF
Sbjct: 3 IESVLSKVPPVWAAAVTAAVVMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIGNF 62
Query: 72 KELVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIR 131
KE+VSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGP+VRVAVSDPDMIR
Sbjct: 63 KEVVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIR 122
Query: 132 EILTTNSEFYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEE 191
EI TTNS FYEKNEAHPLVKQLEGDGLLTLK QKWALHRKIISPTFHM+NLKLLIP+VEE
Sbjct: 123 EIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLVEE 182
Query: 192 SVVDMVEKWSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQM 251
SVV+MVEKWS+ A+ T S EVEI+VF+CFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQM
Sbjct: 183 SVVEMVEKWSSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQM 242
Query: 252 ILASEAFQKVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPK 311
ILAS+AF KVFIPGYRFLPTRTNI+SWKLDKEIKKSLMKLIEGRK NSEKNW MDEK PK
Sbjct: 243 ILASQAFHKVFIPGYRFLPTRTNINSWKLDKEIKKSLMKLIEGRKWNSEKNWGMDEKRPK 302
Query: 312 DLLGLMIQASNSSSCSSLNNN--ITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQ 371
DLLGLMIQAS+ ++ NNN ITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQ
Sbjct: 303 DLLGLMIQASSLKYNNNNNNNNSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQ 362
Query: 372 WQIQARDEVLSVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGR 431
WQIQARDEV +VCG RALPS+ DVSK KILSMILNESLRLYPPTVATIRRAK DVELGGR
Sbjct: 363 WQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVELGGR 422
Query: 432 MIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIG 491
MIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLG RTCIG
Sbjct: 423 MIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGARTCIG 482
Query: 492 QNLALLQAKLAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 532
QNLA+L+AKL M IILQRF+FRLAPTYQHAPTVLMLLYPQYGAPI+FQKI
Sbjct: 483 QNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKI 532
BLAST of Lcy03g013350.1 vs. TAIR 10
Match:
AT2G26710.1 (Cytochrome P450 superfamily protein )
HSP 1 Score: 754.2 bits (1946), Expect = 7.2e-218
Identity = 370/519 (71.29%), Postives = 433/519 (83.43%), Query Frame = 0
Query: 16 KVPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASS 75
KV VL ++ ++ K LWW+PR+IEEHF +QGIRGPPY FFIGN KELV MML ASS
Sbjct: 12 KVLVLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASS 71
Query: 76 HPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSEFYEK 135
HPMP FSHNILPRVLSFYHHW+KIYG+TFL+WFGP R+ V+DPD+IREI + SEFYEK
Sbjct: 72 HPMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIF-SKSEFYEK 131
Query: 136 NEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSAT 195
NEAHPLVKQLEGDGLL+LK +KWA HRKIISPTFHMENLKLL+PVV +SV DMV+KWS
Sbjct: 132 NEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDK 191
Query: 196 ADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFI 255
+ +GEVE++V++ FQ LTEDVI++TAFGSSYEDG+A+F+LQ QQM+L +EAFQKVFI
Sbjct: 192 LSE--NGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFI 251
Query: 256 PGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGP--KDLLGLMIQAS 315
PGYRF PTR N+ SWKLDKEI+KSL+KLIE R+ N+ + K P KDLLGLMIQA
Sbjct: 252 PGYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQA- 311
Query: 316 NSSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSV 375
N+TV DIVEECKSFFFAGKQTTSNLLTWTT+LL+MHP+WQ +ARDEVL V
Sbjct: 312 ---------KNVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRV 371
Query: 376 CGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGTEFLIPI 435
CG R +P++D V KLK LSMILNESLRLYPP VATIRRAK DV+LGG IP GTE LIPI
Sbjct: 372 CGSRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPI 431
Query: 436 LAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAM 495
+AVHHDQ+IWGND +EFNP RF++GV RA HP GFIPFGLGVRTCIGQNLA+LQAKL +
Sbjct: 432 IAVHHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTL 491
Query: 496 TIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIS 533
+++QRF+F LAPTYQHAPTVLMLLYPQ+GAPI F++++
Sbjct: 492 AVMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRLT 516
BLAST of Lcy03g013350.1 vs. TAIR 10
Match:
AT3G14620.1 (cytochrome P450, family 72, subfamily A, polypeptide 8 )
HSP 1 Score: 407.5 bits (1046), Expect = 1.6e-113
Identity = 213/521 (40.88%), Postives = 324/521 (62.19%), Query Frame = 0
Query: 17 VPVLCAVVVILCAKMAV-----ALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMML 76
V V AVVV+ + + W +P++ E + RQG+ G P+ F +G+ K SM+
Sbjct: 9 VAVAAAVVVVTTVTVWIWKGLNVAWLRPKKNEAYLKRQGLSGTPFTFLVGDIKREASMVE 68
Query: 77 AASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSE 136
S P+ N + + RV+ K +G T +W GP+ V V+ P+ I+++L +
Sbjct: 69 QEKSRPI-NLTDDYTHRVMPLIQQTVKDHGKTSYMWMGPIASVIVTKPEHIKDVLNRVYD 128
Query: 137 FYEKNEAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEK 196
F K HP+V +L G+ + +KW+ HRKII+P+FH+E LK++IP ES +M+ K
Sbjct: 129 F-PKPPVHPIV-ELFATGVALYEGEKWSKHRKIINPSFHLEKLKIMIPAFYESCSEMISK 188
Query: 197 WSATADDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQ 256
W + S EI+V+ LT DVI++TAFGSSYE+GK IF+LQ +Q +A +
Sbjct: 189 WEKLVTEQGSSN-EIDVWPYLGDLTSDVISRTAFGSSYEEGKRIFELQEEQGRRVLKALE 248
Query: 257 KVFIPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQ 316
FIPG RFLPT+ N+ +++KE+K L ++I R ++ E DLLG++++
Sbjct: 249 LAFIPGMRFLPTKNNLRMRQINKEVKSRLREIIMKR----QRGMDTGEAPKNDLLGILLE 308
Query: 317 ASNSSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVL 376
SNS ++ +++ D+VEEC+ F FAG++TT+ LL WT ++L+ H +WQ QAR+E+L
Sbjct: 309 -SNSG-----DHGMSIEDVVEECRLFHFAGQETTAVLLVWTMIMLSHHQKWQDQAREEIL 368
Query: 377 SVCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRM-IPRGTEFL 436
V G P+ D +S+LK +SMILNE LRLYPP + R +++ +LG M +P G + +
Sbjct: 369 KVIGKNNKPNFDALSRLKTMSMILNEVLRLYPPGILLGRTVEKETKLGEDMTLPGGAQVV 428
Query: 437 IPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAK 496
IP+L VH D +WG D EFNP RF++G+ +A + F+PFG G R C GQN AL++AK
Sbjct: 429 IPVLMVHRDPELWGEDVHEFNPERFADGISKATKNQVSFLPFGWGPRFCPGQNFALMEAK 488
Query: 497 LAMTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 532
+A+ +ILQRFSF L+P+Y HAP ++ L+PQ+GAP+IF +
Sbjct: 489 MALVLILQRFSFELSPSYTHAPHTVLTLHPQFGAPLIFHML 515
BLAST of Lcy03g013350.1 vs. TAIR 10
Match:
AT3G14610.1 (cytochrome P450, family 72, subfamily A, polypeptide 7 )
HSP 1 Score: 402.1 bits (1032), Expect = 6.9e-112
Identity = 208/515 (40.39%), Postives = 317/515 (61.55%), Query Frame = 0
Query: 17 VPVLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSH 76
+PVL AVVV+ ++ +W KP+ +E RQG+ G PY +G+ K V MM+ A S
Sbjct: 9 LPVLVAVVVLWTWRIVKWVWIKPKMLESSLKRQGLTGTPYTPLVGDIKRNVDMMMEARSK 68
Query: 77 PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSEFYEKN 136
P+ N + +I PR+L +G TF IW GP+ + +++P+ I+E+ ++F EK
Sbjct: 69 PI-NVTDDITPRLLPLALKMLNSHGKTFFIWIGPLPTIVITNPEQIKEVFNKVNDF-EKA 128
Query: 137 EAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSATA 196
PL++ L G GL + K KWA HR+II+P FH+E +K +IP ++V +W
Sbjct: 129 STFPLIRLLAG-GLASYKGDKWASHRRIINPAFHLEKIKNMIPAFYHCCSEVVCQWEKLF 188
Query: 197 DDTASGEVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFIP 256
D S +E++V+ +T DVI+ TAFGSSY++G+ IF+LQ + L ++AF+K +IP
Sbjct: 189 TDKES-PLEVDVWPWLVNMTADVISHTAFGSSYKEGQRIFQLQGELAELIAQAFKKSYIP 248
Query: 257 GYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQASNSS 316
G RF PT++N +D+E+ L ++ R EK E DLLG++++ S+
Sbjct: 249 GSRFYPTKSNRRMKAIDREVDVILRGIVSKR----EKAREAGEPANDDLLGILLE---SN 308
Query: 317 SCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSVCGP 376
S S N ++V D+++ECK F+FAG++TTS LL WT VLL+ H WQ +AR+EV+ V G
Sbjct: 309 SEESQGNGMSVEDVMKECKLFYFAGQETTSVLLVWTMVLLSHHQDWQARAREEVMQVLGE 368
Query: 377 RALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGTEFLIPILAV 436
P + ++ LK+++MI NE LRLYPP R ++++LG +P G + +P + V
Sbjct: 369 NNKPDMESLNNLKVMTMIFNEVLRLYPPVAQLKRVVNKEMKLGELTLPAGIQIYLPTILV 428
Query: 437 HHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAMTII 496
D +WG+DA++F P RF +G+ +A + F PFG G R CIGQN A+L+AK+AM +I
Sbjct: 429 QRDTELWGDDAADFKPERFRDGLSKATKNQVSFFPFGWGPRICIGQNFAMLEAKMAMALI 488
Query: 497 LQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 532
LQ+FSF L+P+Y HAP +M PQ+GA +I K+
Sbjct: 489 LQKFSFELSPSYVHAPQTVMTTRPQFGAHLILHKL 512
BLAST of Lcy03g013350.1 vs. TAIR 10
Match:
AT1G75130.1 (cytochrome P450, family 721, subfamily A, polypeptide 1 )
HSP 1 Score: 397.1 bits (1019), Expect = 2.2e-110
Identity = 213/520 (40.96%), Postives = 313/520 (60.19%), Query Frame = 0
Query: 19 VLCAVVVILCAKMAVALWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSHPM 78
+L V L + + W P RI+ HF +Q + GP YR F GN E+ + A S P+
Sbjct: 6 ILVLVFFFLVFRFIYSNIWVPWRIQSHFKKQSVTGPSYRIFSGNSGEVSRLTAEAKSKPI 65
Query: 79 PN--FSHNILPRVLSFYHHWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSEFYEKN 138
P+ H + RV YH W ++YG TFL WFG VA SDP +IRE LTT F ++
Sbjct: 66 PSGRNPHEFVHRVAPHYHEWSRVYGKTFLYWFGSKPVVATSDPRLIREALTTGGSF-DRI 125
Query: 139 EAHPLVKQLEGDGLLTLKDQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSATA 198
+PL K L GL L+ +WA HR+I F ME LK +P + S + ++EKW
Sbjct: 126 GHNPLSKLLYAQGLPGLRGDQWAFHRRIAKQAFTMEKLKRWVPQMVTSTMMLMEKW---- 185
Query: 199 DDTASG--EVEIEVFDCFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVF 258
+D +G E+E+EV L+ +++++TAFG+S E+GK IF+LQ + M L V+
Sbjct: 186 EDMRNGGEEIELEVHKEMHNLSAEMLSRTAFGNSVEEGKGIFELQERMMRLFYLVRWSVY 245
Query: 259 IPGYRFLPTRTNISSWKLDKEIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQA-- 318
IPG+RF P++TN W+++K+I+ S++KLIE K EK+ G ++QA
Sbjct: 246 IPGFRFFPSKTNREIWRIEKQIRVSILKLIENNKTAVEKS------------GTLLQAFM 305
Query: 319 SNSSSCSSLNNNITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLS 378
S ++ + + + ++ +ECK+F+FA K+TT+NL+T+ VLLAM+ +WQ AR+EV+
Sbjct: 306 SPYTNQNGQEEKLGIEEVTDECKTFYFAAKETTANLMTFVLVLLAMNQEWQNIAREEVIC 365
Query: 379 VCGPRALPSRDDVSKLKILSMILNESLRLYPPTVATIRRAKEDVELGGRMIPRGTEFLIP 438
V G LP+ D + LK LSMI+NE+LRLYPP + R + +LG IP GT+ +
Sbjct: 366 VLGQTGLPTLDILQDLKTLSMIINETLRLYPPAMTLNRDTLKRAKLGDLDIPAGTQLYLS 425
Query: 439 ILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLA 498
++A+HHD+ WG+DA EFNP RF + A +PFGLG RTC+GQNLA+ +AK
Sbjct: 426 VVAMHHDKETWGDDAEEFNPRRFED----PKKQSALLVPFGLGPRTCVGQNLAVNEAKTV 485
Query: 499 MTIILQRFSFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIS 533
+ IL+ +SFRL+P+Y HAP + + L PQ GA ++F +IS
Sbjct: 486 LATILKYYSFRLSPSYAHAPVLFVTLQPQNGAHLLFTRIS 504
BLAST of Lcy03g013350.1 vs. TAIR 10
Match:
AT3G14680.1 (cytochrome P450, family 72, subfamily A, polypeptide 14 )
HSP 1 Score: 396.7 bits (1018), Expect = 2.9e-110
Identity = 206/497 (41.45%), Postives = 307/497 (61.77%), Query Frame = 0
Query: 35 LWWKPRRIEEHFLRQGIRGPPYRFFIGNFKELVSMMLAASSHPMPNFSHNILPRVLSFYH 94
+W+ P+ +E RQG+ G Y IG+FK+++SM + A+S P+ + +I PRV+
Sbjct: 28 VWFTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFIEATSKPIKP-TDDITPRVMPHPL 87
Query: 95 HWKKIYGSTFLIWFGPMVRVAVSDPDMIREILTTNSEFYEKNEAHPLVKQLEGDGLLTLK 154
K +G T L WFGP+ + + DP+ I+E+ +F +K PL K L G GL++
Sbjct: 88 QMLKTHGRTNLTWFGPIPTITIMDPEQIKEVFNKVYDF-QKAHTFPLSKIL-GTGLVSYD 147
Query: 155 DQKWALHRKIISPTFHMENLKLLIPVVEESVVDMVEKWSATADDTASGEVEIEVFDCFQT 214
KWA HR+II+P FH+E +K ++ V ES ++V +W D S E++V+ +
Sbjct: 148 GDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDKLVSDKGS-SCEVDVWPGLTS 207
Query: 215 LTEDVITKTAFGSSYEDGKAIFKLQTQQMILASEAFQKVFIPGYRFLPTRTNISSWKLDK 274
+T DVI++TAFGSSY +G IF+LQ + L +AFQK FIPGY +LPT+ N +
Sbjct: 208 MTADVISRTAFGSSYREGHRIFELQAELAQLVMQAFQKFFIPGYIYLPTKGNRRMKTAAR 267
Query: 275 EIKKSLMKLIEGRKGNSEKNWAMDEKGPKDLLGLMIQASNSSSCSSLNNNITVHDIVEEC 334
EI+ L +I R+ E A E DLLG++++ S+ + N ++ D++EEC
Sbjct: 268 EIQDILRGIINKRERARESGEAPSE----DLLGILLE---SNLGQTEGNGMSTEDMMEEC 327
Query: 335 KSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVLSVCGPRALPSRDDVSKLKILSMI 394
K F+ AG++TTS LL WT VLL+ H WQ +AR+EV V G + P + +++LK+++MI
Sbjct: 328 KLFYLAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKQ-PDTEGLNQLKVMTMI 387
Query: 395 LNESLRLYPPTVATIRRAKEDVELGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGR 454
L E LRLYPP V R ++++LG +P G + +P+L VH D +WGNDA EF P R
Sbjct: 388 LYEVLRLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELWGNDAGEFKPER 447
Query: 455 FSEGVGRAGNHPAGFIPFGLGVRTCIGQNLALLQAKLAMTIILQRFSFRLAPTYQHAPTV 514
F +G+ +A + F PF G R CIGQN LL+AK+AM++ILQRFSF L+P+Y HAP
Sbjct: 448 FKDGLSKATKNQVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSFELSPSYVHAPYT 507
Query: 515 LMLLYPQYGAPIIFQKI 532
++ LYPQ+GA ++ K+
Sbjct: 508 IITLYPQFGAHLMLHKL 512
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
O48786 | 1.0e-216 | 71.29 | Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1 | [more] |
B9X287 | 2.0e-188 | 62.33 | Cytochrome P450 734A6 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A6 PE=2 ... | [more] |
Q6Z6D6 | 1.1e-175 | 56.33 | Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 ... | [more] |
Q69XM6 | 6.6e-168 | 54.38 | Cytochrome P450 734A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A4 PE=2 ... | [more] |
Q8LIF2 | 1.3e-150 | 50.98 | Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A5 PE=2 ... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1L528 | 1.3e-270 | 87.74 | cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111499208 PE=3 SV=1 | [more] |
A0A6J1EZI1 | 2.4e-269 | 88.17 | cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111440975 PE=3 SV... | [more] |
A0A1S3CMW0 | 5.5e-258 | 83.05 | cytochrome P450 734A1-like OS=Cucumis melo OX=3656 GN=LOC103502730 PE=3 SV=1 | [more] |
A0A0A0LU20 | 2.7e-257 | 83.65 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G305710 PE=3 SV=1 | [more] |
A0A6J1H9G1 | 2.8e-254 | 84.50 | cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111461725 PE=3 SV... | [more] |
Match Name | E-value | Identity | Description | |
AT2G26710.1 | 7.2e-218 | 71.29 | Cytochrome P450 superfamily protein | [more] |
AT3G14620.1 | 1.6e-113 | 40.88 | cytochrome P450, family 72, subfamily A, polypeptide 8 | [more] |
AT3G14610.1 | 6.9e-112 | 40.39 | cytochrome P450, family 72, subfamily A, polypeptide 7 | [more] |
AT1G75130.1 | 2.2e-110 | 40.96 | cytochrome P450, family 721, subfamily A, polypeptide 1 | [more] |
AT3G14680.1 | 2.9e-110 | 41.45 | cytochrome P450, family 72, subfamily A, polypeptide 14 | [more] |