Lag0032736 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0032736 vs. NCBI nr
Match: XP_022995255.1 (uncharacterized protein LOC111490857 [Cucurbita maxima] >XP_022995256.1 uncharacterized protein LOC111490857 [Cucurbita maxima])

HSP 1 Score: 318.5 bits (815), Expect = 3.4e-83
Identity = 155/169 (91.72%), Postives = 163/169 (96.45%), Query Frame = 0

Query: 1   MKYVKPLNLFHDLLKRNALERGRFLGLDVGDKYVGLAVSDPDNKIASPLSVLLRKKTNMH 60
           MKYV+PLNLF DLLK NALERGRFLGLDVGDKYVGLAVSDPDNKIASPLSVL RKKTNMH
Sbjct: 1   MKYVRPLNLFRDLLKGNALERGRFLGLDVGDKYVGLAVSDPDNKIASPLSVLFRKKTNMH 60

Query: 61  LVAQDFQRLISELSLAGFIVGYPFDGKRPNADAVQVKIFIDDLCKTGKLEGVKYTFWNEC 120
           L+A DFQRLISELSL+GFIVGYPFDG+R NADAVQVKIFIDDLCKTGKLEGVKYTFW+EC
Sbjct: 61  LMALDFQRLISELSLSGFIVGYPFDGQRRNADAVQVKIFIDDLCKTGKLEGVKYTFWDEC 120

Query: 121 FTSKNVELLIKPLKLPPILSKTIIDKFAAVGILQGYLDYFNRKSDLEER 170
           FTSKNVELLIKPLKLPPILSKTIIDKFAAVGILQGYLD+FNR+ +LEER
Sbjct: 121 FTSKNVELLIKPLKLPPILSKTIIDKFAAVGILQGYLDHFNRRPELEER 169

BLAST of Lag0032736 vs. NCBI nr
Match: XP_022957830.1 (uncharacterized protein LOC111459253 [Cucurbita moschata] >XP_022957831.1 uncharacterized protein LOC111459253 [Cucurbita moschata] >XP_022957832.1 uncharacterized protein LOC111459253 [Cucurbita moschata])

HSP 1 Score: 317.4 bits (812), Expect = 7.5e-83
Identity = 154/169 (91.12%), Postives = 163/169 (96.45%), Query Frame = 0

Query: 1   MKYVKPLNLFHDLLKRNALERGRFLGLDVGDKYVGLAVSDPDNKIASPLSVLLRKKTNMH 60
           MKYV+PLNLF DLLK NALERGRFLGLDVGDKYVGLAVSDPDNKIASPLSVL RKKTNMH
Sbjct: 1   MKYVRPLNLFRDLLKGNALERGRFLGLDVGDKYVGLAVSDPDNKIASPLSVLFRKKTNMH 60

Query: 61  LVAQDFQRLISELSLAGFIVGYPFDGKRPNADAVQVKIFIDDLCKTGKLEGVKYTFWNEC 120
           L+A DFQRLISELSL+GFIVGYPFDG+R NADAVQVKIFIDDLCKTGKLEGVKYTFW+EC
Sbjct: 61  LMALDFQRLISELSLSGFIVGYPFDGQRRNADAVQVKIFIDDLCKTGKLEGVKYTFWDEC 120

Query: 121 FTSKNVELLIKPLKLPPILSKTIIDKFAAVGILQGYLDYFNRKSDLEER 170
           FTSKNVELLIKPLKLPPILSKTIIDKFAAVGILQGYLD+FNR+ +L+ER
Sbjct: 121 FTSKNVELLIKPLKLPPILSKTIIDKFAAVGILQGYLDHFNRRPELDER 169

BLAST of Lag0032736 vs. NCBI nr
Match: XP_023533829.1 (uncharacterized protein LOC111795559 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 316.2 bits (809), Expect = 1.7e-82
Identity = 154/169 (91.12%), Postives = 162/169 (95.86%), Query Frame = 0

Query: 1   MKYVKPLNLFHDLLKRNALERGRFLGLDVGDKYVGLAVSDPDNKIASPLSVLLRKKTNMH 60
           MKYV+PLNLF DLLK NALERGRFLGLDVGDKYVGLAVSDPDNKIASPLSVL RKKTNMH
Sbjct: 1   MKYVRPLNLFRDLLKGNALERGRFLGLDVGDKYVGLAVSDPDNKIASPLSVLFRKKTNMH 60

Query: 61  LVAQDFQRLISELSLAGFIVGYPFDGKRPNADAVQVKIFIDDLCKTGKLEGVKYTFWNEC 120
           L+A DFQRLISELSL+GFIVGYPFDG+R NADAVQVKIFIDDLCKTGKLEGVKYTFW+EC
Sbjct: 61  LMALDFQRLISELSLSGFIVGYPFDGQRRNADAVQVKIFIDDLCKTGKLEGVKYTFWDEC 120

Query: 121 FTSKNVELLIKPLKLPPILSKTIIDKFAAVGILQGYLDYFNRKSDLEER 170
           FTSKNVELLIKPLKLPPILSKTIIDKFAAVGILQ YLD+FNR+ +LEER
Sbjct: 121 FTSKNVELLIKPLKLPPILSKTIIDKFAAVGILQAYLDHFNRRPELEER 169

BLAST of Lag0032736 vs. NCBI nr
Match: KAG7036046.1 (hypothetical protein SDJN02_02846 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 303.1 bits (775), Expect = 1.5e-78
Identity = 154/194 (79.38%), Postives = 162/194 (83.51%), Query Frame = 0

Query: 1   MKYVKPLNLFHDLLKRNALERGRFLGLDVGDKYVGLAVSDPDNKIASPLSVLLRKKTNMH 60
           MKYV+PLNLF DLLK NALERGRFLGLDVGDKYVGLAVSDPDNKIASPLSVL RKKTNMH
Sbjct: 1   MKYVRPLNLFRDLLKGNALERGRFLGLDVGDKYVGLAVSDPDNKIASPLSVLFRKKTNMH 60

Query: 61  LVAQDFQRLISELSLAGFIVGYPFDGKRPNAD-------------------------AVQ 120
           L+A DFQRLISELSL+GFIVGYPFDG+R N D                         AVQ
Sbjct: 61  LMALDFQRLISELSLSGFIVGYPFDGQRRNVDVCSLSLEIHHYSLSSLLDITLFCIQAVQ 120

Query: 121 VKIFIDDLCKTGKLEGVKYTFWNECFTSKNVELLIKPLKLPPILSKTIIDKFAAVGILQG 170
           VKIFIDDLCKTGKLEGVKYTFW+ECFTSKNVELLIKPLKLPPILSKTIIDKFAAVGILQG
Sbjct: 121 VKIFIDDLCKTGKLEGVKYTFWDECFTSKNVELLIKPLKLPPILSKTIIDKFAAVGILQG 180

BLAST of Lag0032736 vs. NCBI nr
Match: XP_022159849.1 (uncharacterized protein LOC111026145 [Momordica charantia] >XP_022159850.1 uncharacterized protein LOC111026145 [Momordica charantia] >XP_022159851.1 uncharacterized protein LOC111026145 [Momordica charantia])

HSP 1 Score: 299.7 bits (766), Expect = 1.6e-77
Identity = 145/166 (87.35%), Postives = 155/166 (93.37%), Query Frame = 0

Query: 1   MKYVKPLNLFHDLLKRNALERGRFLGLDVGDKYVGLAVSDPDNKIASPLSVLLRKKTNMH 60
           MKYVKPLN+F DLL RN LERGRFLGLDVGDKYVGLAVSDPDN IASPLSVLLRKKT MH
Sbjct: 1   MKYVKPLNMFRDLLTRNPLERGRFLGLDVGDKYVGLAVSDPDNLIASPLSVLLRKKTKMH 60

Query: 61  LVAQDFQRLISELSLAGFIVGYPFDGKRPNADAVQVKIFIDDLCKTGKLEGVKYTFWNEC 120
           L+A+D QRLISELSLAGFIVGYPFD +R NADAVQVKIFIDDLCKTG LEGVKYTFW+EC
Sbjct: 61  LMARDLQRLISELSLAGFIVGYPFDRQRRNADAVQVKIFIDDLCKTGTLEGVKYTFWDEC 120

Query: 121 FTSKNVELLIKPLKLPPILSKTIIDKFAAVGILQGYLDYFNRKSDL 167
           FTSKNVELLIKPL+LPP+LSKTI+DKFAAVGILQGYLDY NR+ +L
Sbjct: 121 FTSKNVELLIKPLELPPVLSKTIVDKFAAVGILQGYLDYHNRRPEL 166

BLAST of Lag0032736 vs. ExPASy Swiss-Prot
Match: B9MRK7 (Putative pre-16S rRNA nuclease OS=Caldicellulosiruptor bescii (strain ATCC BAA-1888 / DSM 6725 / Z-1320) OX=521460 GN=Athe_1211 PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.2e-13
Identity = 48/140 (34.29%), Postives = 76/140 (54.29%), Query Frame = 0

Query: 23  RFLGLDVGDKYVGLAVSDPDNKIASP-LSVLLRKKTNMHLVAQDFQRLISELSLAGFIVG 82
           R L LD+G+  VG+A+SDP    A P +++ LR K     + ++  ++    ++   ++G
Sbjct: 2   RILCLDIGNSRVGVAISDPLKITAQPVMTIELRNKD----LFEELDKIFQGYNIEKVVIG 61

Query: 83  YPFDGKRPNADAVQVKIFIDDLCKTGKLEGVKYTFWNECFTSKNVELLIKPLKLPPILSK 142
           YP     P+    ++K   +   K G    V+   W+E F++K VE +I   +L     K
Sbjct: 62  YPLSKLHPDQKDEKLKKIDEISEKIGSRYNVEIVKWDERFSTKAVERVIDE-ELNWKRKK 121

Query: 143 TIIDKFAAVGILQGYLDYFN 162
            IIDK AAV ILQGYLD++N
Sbjct: 122 KIIDKVAAVYILQGYLDFYN 136

BLAST of Lag0032736 vs. ExPASy Swiss-Prot
Match: A4XKC6 (Putative pre-16S rRNA nuclease OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903 / Tp8T 6331) OX=351627 GN=Csac_1776 PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 3.8e-13
Identity = 45/140 (32.14%), Postives = 76/140 (54.29%), Query Frame = 0

Query: 23  RFLGLDVGDKYVGLAVSDPDNKIASPLSVLLRKKTNMHLVAQDFQRLISELSLAGFIVGY 82
           R L LD+G + +G+A+SDP    A P  VL  +K +++       ++ +E  +   ++GY
Sbjct: 2   RILCLDIGSRRIGVAISDPLKIAAQPFCVLDLQKEDLY---SCLDKIFNEHQIEKIVIGY 61

Query: 83  PFDGKRPNADAVQVKIFIDDLC-KTGKLEGVKYTFWNECFTSKNVELLIKPLKLPPILSK 142
           P     P+  A +    ID++C +  +   V    W+E F++K VE +++   +     K
Sbjct: 62  PVSKFHPD-KATEKLQKIDEICSELERRYKVGIIKWDERFSTKAVERILREENVSWQKRK 121

Query: 143 TIIDKFAAVGILQGYLDYFN 162
            ++DK AAV ILQGYLD+ N
Sbjct: 122 KVVDKLAAVYILQGYLDFIN 137

BLAST of Lag0032736 vs. ExPASy Swiss-Prot
Match: B6IN25 (Putative pre-16S rRNA nuclease OS=Rhodospirillum centenum (strain ATCC 51521 / SW) OX=414684 GN=RC1_1518 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.5e-12
Identity = 48/148 (32.43%), Postives = 74/148 (50.00%), Query Frame = 0

Query: 14  LKRNALERGRFLGLDVGDKYVGLAVSDPDNKIASPLSVLLRKKTNMHLVAQDFQRLISEL 73
           LK +    GR +GLD+G+K +GLAVSDP   +ASP+  + R K      AQ+  R + + 
Sbjct: 9   LKASLPRNGRLMGLDLGEKTIGLAVSDPGLSVASPVGTIRRTKFTQD--AQELARAMRDR 68

Query: 74  SLAGFIVGYPFDGKRPNADAVQ-VKIFIDDLCKTGKLEGV--KYTFWNECFTSKNVELLI 133
            +   +VG P +         Q V+ F   L +  +L G   +  FW+E  ++  VE ++
Sbjct: 69  DVRALVVGLPVNMDGTEGPRCQSVREFSRLLLQRPQLFGFEPEIAFWDERLSTSAVERMM 128

Query: 134 KPLKLPPILSKTIIDKFAAVGILQGYLD 159
               +       ++DK AA  ILQG LD
Sbjct: 129 IGWDMTRKRRDEVVDKMAAAYILQGALD 154

BLAST of Lag0032736 vs. ExPASy Swiss-Prot
Match: C4Z578 (Putative pre-16S rRNA nuclease OS=Lachnospira eligens (strain ATCC 27750 / DSM 3376 / VPI C15-48 / C15-B4) OX=515620 GN=EUBELI_00774 PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 5.5e-12
Identity = 42/137 (30.66%), Postives = 70/137 (51.09%), Query Frame = 0

Query: 23  RFLGLDVGDKYVGLAVSDPDNKIASPLSVLLRKKTN-MHLVAQDFQRLISELSLAGFIVG 82
           R +GLDVG K VG+A+SDP    A P   + RK+ N +       +++ISE  +   +VG
Sbjct: 2   RIIGLDVGTKTVGVALSDPLGITAQPFETITRKENNKLRRTYARIEQIISEYDVTEIVVG 61

Query: 83  YPFDGKRPNADAVQVKIFIDDLCKTGKLEGVKYTFWNECFTSKNVELLIKPLKLPPILSK 142
           YP      +    + K   +      +  G+  T W+E  T+   + +++   +     K
Sbjct: 62  YP--KNMDDTIGERAKACEEFAAALERRTGLPVTLWDERLTTVEADEVLEECGMRRENRK 121

Query: 143 TIIDKFAAVGILQGYLD 159
           T+ID+ AAV IL+GY++
Sbjct: 122 TVIDQLAAVFILRGYME 136

BLAST of Lag0032736 vs. ExPASy Swiss-Prot
Match: B8I4S7 (Putative pre-16S rRNA nuclease OS=Ruminiclostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) OX=394503 GN=Ccel_2239 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.6e-11
Identity = 45/147 (30.61%), Postives = 74/147 (50.34%), Query Frame = 0

Query: 23  RFLGLDVGDKYVGLAVSDPDNKIASPLSVLLRKKTNMHLVAQDFQRLISELSLAGFIVGY 82
           R LG+D GD  +G+A+SDP    A  L  +++ K ++         +I+E S+   ++GY
Sbjct: 2   RILGIDYGDSRIGVAISDPMGWTAQGLE-MIKSKDSLKKAVLRLSEIINEYSVTDIVIGY 61

Query: 83  PFD---GKRPNADAVQVKIFIDDLCKTGKLEGVKYTFWNECFTSKNVELLIKPLKLPPIL 142
           P +    K P  +  + + FI  +   G+   +K   W+E  T+ +    +  L +    
Sbjct: 62  PINMNGTKGPRTE--RTEEFIKKISDLGQFNIIK---WDERLTTVSAHRTMNELGIKASK 121

Query: 143 SKTIIDKFAAVGILQGYLDYF--NRKS 165
            K I+D  +AV ILQGYLD    N+KS
Sbjct: 122 KKGIVDTMSAVLILQGYLDRIAGNKKS 142