Lag0005533 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0005533
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationchr6: 20899489 .. 20899791 (+)
RNA-Seq ExpressionLag0005533
SyntenyLag0005533
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGCAGAAAAATATGGTTTTTGATGACATTGCTTATTCACTGATTGTTTATGCTAAAGTAAAAATTGGGATTATAACTTATGCATTGGAAGTGTTTGAAGAAATGACCAAAAGAGGGTTTCAAGCAAATTCTTTCATTTATACCTTGTTCATTGGTGCCCATTGTAGAGAGGGAAAGATTGAGGAAGCTCATTGCTTGATGCAAGAGATGGAAAACATAGGTTGGAAGCCATATCCTGAAACCTTCAATCTTCTCATTGAAGGGTCTGTTTCGTCCGGAGTTCAATATTTATGTTCATGA

mRNA sequence

ATGTTGCAGAAAAATATGGTTTTTGATGACATTGCTTATTCACTGATTGTTTATGCTAAAGTAAAAATTGGGATTATAACTTATGCATTGGAAGTGTTTGAAGAAATGACCAAAAGAGGGTTTCAAGCAAATTCTTTCATTTATACCTTGTTCATTGGTGCCCATTGTAGAGAGGGAAAGATTGAGGAAGCTCATTGCTTGATGCAAGAGATGGAAAACATAGGTTGGAAGCCATATCCTGAAACCTTCAATCTTCTCATTGAAGGGTCTGTTTCGTCCGGAGTTCAATATTTATGTTCATGA

Coding sequence (CDS)

ATGTTGCAGAAAAATATGGTTTTTGATGACATTGCTTATTCACTGATTGTTTATGCTAAAGTAAAAATTGGGATTATAACTTATGCATTGGAAGTGTTTGAAGAAATGACCAAAAGAGGGTTTCAAGCAAATTCTTTCATTTATACCTTGTTCATTGGTGCCCATTGTAGAGAGGGAAAGATTGAGGAAGCTCATTGCTTGATGCAAGAGATGGAAAACATAGGTTGGAAGCCATATCCTGAAACCTTCAATCTTCTCATTGAAGGGTCTGTTTCGTCCGGAGTTCAATATTTATGTTCATGA

Protein sequence

MLQKNMVFDDIAYSLIVYAKVKIGIITYALEVFEEMTKRGFQANSFIYTLFIGAHCREGKIEEAHCLMQEMENIGWKPYPETFNLLIEGSVSSGVQYLCS
Homology
[Close]
BLAST of Lag0005533 vs. NCBI nr
Match: XP_038875410.1 (pentatricopeptide repeat-containing protein At1g66345, mitochondrial [Benincasa hispida] >XP_038875411.1 pentatricopeptide repeat-containing protein At1g66345, mitochondrial [Benincasa hispida])

HSP 1 Score: 165.2 bits (417), Expect = 2.8e-37
Identity = 81/94 (86.17%), Postives = 85/94 (90.43%), Query Frame = 0 

Query: 1   MLQKNMVFDDIAYSLIVYAKVKIGIITYALEVFEEMTKRGFQANSFIYTLFIGAHCREGK 60
           MLQKNMV DDIAYSLIVYAKVK G IT A EVFEEM+KRGFQANSFIYTLFIG HCREGK
Sbjct: 291 MLQKNMVLDDIAYSLIVYAKVKTGSITSAWEVFEEMSKRGFQANSFIYTLFIGVHCREGK 350

Query: 61  IEEAHCLMQEMENIGWKPYPETFNLLIEGSVSSG 95
           IEEAHCLMQEMEN+G KPYPETF+LLIEG  +SG
Sbjct: 351 IEEAHCLMQEMENMGLKPYPETFSLLIEGYATSG 384

[Close]
BLAST of Lag0005533 vs. NCBI nr
Match: XP_031740872.1 (pentatricopeptide repeat-containing protein At1g66345, mitochondrial [Cucumis sativus] >KGN51057.1 hypothetical protein Csa_008589 [Cucumis sativus])

HSP 1 Score: 160.2 bits (404), Expect = 9.1e-36
Identity = 78/94 (82.98%), Postives = 82/94 (87.23%), Query Frame = 0 

Query: 1   MLQKNMVFDDIAYSLIVYAKVKIGIITYALEVFEEMTKRGFQANSFIYTLFIGAHCREGK 60
           MLQKNMV DDIAYSLIVYAKVK G IT   EVFEEM++RGFQANSFIYTLFIG HCR GK
Sbjct: 290 MLQKNMVLDDIAYSLIVYAKVKTGSITSTWEVFEEMSERGFQANSFIYTLFIGVHCRGGK 349

Query: 61  IEEAHCLMQEMENIGWKPYPETFNLLIEGSVSSG 95
           +EEAHCLMQEMEN+G KPYPETFNLLIEG   SG
Sbjct: 350 VEEAHCLMQEMENMGLKPYPETFNLLIEGCAISG 383

[Close]
BLAST of Lag0005533 vs. NCBI nr
Match: XP_023550461.1 (pentatricopeptide repeat-containing protein At1g66345, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 157.9 bits (398), Expect = 4.5e-35
Identity = 75/89 (84.27%), Postives = 83/89 (93.26%), Query Frame = 0 

Query: 1   MLQKNMVFDDIAYSLIVYAKVKIGIITYALEVFEEMTKRGFQANSFIYTLFIGAHCREGK 60
           MLQKNM+ DDIAYSLIVYAK+KIG I  A EVF+EM+KRGFQANSFIYTLFIGAHCR+G+
Sbjct: 291 MLQKNMILDDIAYSLIVYAKLKIGNIKSAQEVFDEMSKRGFQANSFIYTLFIGAHCRDGR 350

Query: 61  IEEAHCLMQEMENIGWKPYPETFNLLIEG 90
           IEEAHCLM+EMEN+G KPYPETFNLLIEG
Sbjct: 351 IEEAHCLMEEMENMGLKPYPETFNLLIEG 379

[Close]
BLAST of Lag0005533 vs. NCBI nr
Match: XP_022141355.1 (pentatricopeptide repeat-containing protein At1g66345, mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 157.5 bits (397), Expect = 5.9e-35
Identity = 79/94 (84.04%), Postives = 84/94 (89.36%), Query Frame = 0 

Query: 1   MLQKNMVFDDIAYSLIVYAKVKIGIITYALEVFEEMTKRGFQANSFIYTLFIGAHCREGK 60
           ML KNMV DDIAYSLIVYAKVK G IT A EVFEEM+KRGFQANSFIYTLFIGAHCREGK
Sbjct: 289 MLLKNMVLDDIAYSLIVYAKVKQGNITSAWEVFEEMSKRGFQANSFIYTLFIGAHCREGK 348

Query: 61  IEEAHCLMQEMENIGWKPYPETFNLLIEGSVSSG 95
           IEEA+ LMQEMEN+G KPYPETFNLLIEG  ++G
Sbjct: 349 IEEANSLMQEMENMGLKPYPETFNLLIEGCATAG 382

[Close]
BLAST of Lag0005533 vs. NCBI nr
Match: XP_022141356.1 (pentatricopeptide repeat-containing protein At1g66345, mitochondrial isoform X2 [Momordica charantia])

HSP 1 Score: 157.5 bits (397), Expect = 5.9e-35
Identity = 79/94 (84.04%), Postives = 84/94 (89.36%), Query Frame = 0 

Query: 1   MLQKNMVFDDIAYSLIVYAKVKIGIITYALEVFEEMTKRGFQANSFIYTLFIGAHCREGK 60
           ML KNMV DDIAYSLIVYAKVK G IT A EVFEEM+KRGFQANSFIYTLFIGAHCREGK
Sbjct: 289 MLLKNMVLDDIAYSLIVYAKVKQGNITSAWEVFEEMSKRGFQANSFIYTLFIGAHCREGK 348

Query: 61  IEEAHCLMQEMENIGWKPYPETFNLLIEGSVSSG 95
           IEEA+ LMQEMEN+G KPYPETFNLLIEG  ++G
Sbjct: 349 IEEANSLMQEMENMGLKPYPETFNLLIEGCATAG 382

[Close]
BLAST of Lag0005533 vs. ExPASy Swiss-Prot
Match: Q3ECH5 (Pentatricopeptide repeat-containing protein At1g66345, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g66345 PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 5.0e-21
Identity = 49/94 (52.13%), Postives = 63/94 (67.02%), Query Frame = 0 

Query: 1   MLQKNMVFDDIAYSLIVYAKVKIGIITYALEVFEEMTKRGFQANSFIYTLFIGAHCREGK 60
           +L KNMV D I YS++VYAK K G +  A +VF+EM +RGF ANSF+YT+F+   C +G 
Sbjct: 295 LLMKNMVVDTIGYSIVVYAKAKEGDLVSARKVFDEMLQRGFSANSFVYTVFVRVCCEKGD 354

Query: 61  IEEAHCLMQEMENIGWKPYPETFNLLIEGSVSSG 95
           ++EA  L+ EME  G  PY ETFN LI G    G
Sbjct: 355 VKEAERLLSEMEESGVSPYDETFNCLIGGFARFG 388

[Close]
BLAST of Lag0005533 vs. ExPASy Swiss-Prot
Match: Q9SAK0 (Pentatricopeptide repeat-containing protein At1g79490, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=EMB2217 PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 5.8e-09
Identity = 28/79 (35.44%), Postives = 48/79 (60.76%), Query Frame = 0 

Query: 16  IVYAKVKIGIITYALEVFEEMTKRGFQANSFIYTLFIGAHCREGKIEEAHCLMQEMENIG 75
           ++ +  K G +  AL +++EM K GF+ N  +YT+ I +H + GK+E A  + ++ME  G
Sbjct: 389 LIDSYAKAGKLDTALRLWDEMKKSGFRPNFGLYTMIIESHAKSGKLEVAMTVFKDMEKAG 448

Query: 76  WKPYPETFNLLIEGSVSSG 95
           + P P T++ L+E    SG
Sbjct: 449 FLPTPSTYSCLLEMHAGSG 467

[Close]
BLAST of Lag0005533 vs. ExPASy Swiss-Prot
Match: Q8S8P6 (Pentatricopeptide repeat-containing protein At2g32630 OS=Arabidopsis thaliana OX=3702 GN=At2g32630 PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.3e-08
Identity = 29/94 (30.85%), Postives = 54/94 (57.45%), Query Frame = 0 

Query: 1   MLQKNMVFDDIAYSLIVYAKVKIGIITYALEVFEEMTKRGFQANSFIYTLFIGAHCREGK 60
           M +  +V++ + Y+L++   VK G ++ A ++F+EM +RG +++  +YT  I  +CR+G 
Sbjct: 285 MKKDGVVYNKVTYTLLMELSVKNGKMSDAEKLFDEMRERGIESDVHVYTSLISWNCRKGN 344

Query: 61  IEEAHCLMQEMENIGWKPYPETFNLLIEGSVSSG 95
           ++ A  L  E+   G  P   T+  LI+G    G
Sbjct: 345 MKRAFLLFDELTEKGLSPSSYTYGALIDGVCKVG 378

[Close]
BLAST of Lag0005533 vs. ExPASy Swiss-Prot
Match: Q9FIT7 (Pentatricopeptide repeat-containing protein At5g61990, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g61990 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.7e-08
Identity = 31/94 (32.98%), Postives = 49/94 (52.13%), Query Frame = 0 

Query: 1   MLQKNMVFDDIAYSLIVYAKVKIGIITYALEVFEEMTKRGFQANSFIYTLFIGAHCREGK 60
           M  K +  D  +Y +++    K+G +  A  +F+EM + G   N  IY + +G  CR G+
Sbjct: 618 MRGKGIAPDVFSYGVLINGFSKLGNMQKASSIFDEMVEEGLTPNVIIYNMLLGGFCRSGE 677

Query: 61  IEEAHCLMQEMENIGWKPYPETFNLLIEGSVSSG 95
           IE+A  L+ EM   G  P   T+  +I+G   SG
Sbjct: 678 IEKAKELLDEMSVKGLHPNAVTYCTIIDGYCKSG 711

[Close]
BLAST of Lag0005533 vs. ExPASy Swiss-Prot
Match: Q9FMF6 (Pentatricopeptide repeat-containing protein At5g64320, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g64320 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.7e-08
Identity = 30/82 (36.59%), Postives = 47/82 (57.32%), Query Frame = 0 

Query: 6   MVFDDIAYSLIVYAKVKIGIITYALEVFEEMTKRGFQANSFIYTLFIGAHCREGKIEEAH 65
           +V D   Y+ ++Y   K G++  ALEV  +M  +G + N + YT+ +   C+ GKI+EA+
Sbjct: 385 IVPDVCTYNSLIYGYWKEGLVGLALEVLHDMRNKGCKPNVYSYTILVDGFCKLGKIDEAY 444

Query: 66  CLMQEMENIGWKPYPETFNLLI 88
            ++ EM   G KP    FN LI
Sbjct: 445 NVLNEMSADGLKPNTVGFNCLI 466

The following BLAST results are available for this feature:
BLAST of Lag0005533 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038875410.12.8e-3786.17pentatricopeptide repeat-containing protein At1g66345, mitochondrial [Benincasa ... [more]
XP_031740872.19.1e-3682.98pentatricopeptide repeat-containing protein At1g66345, mitochondrial [Cucumis sa... [more]
XP_023550461.14.5e-3584.27pentatricopeptide repeat-containing protein At1g66345, mitochondrial [Cucurbita ... [more]
XP_022141355.15.9e-3584.04pentatricopeptide repeat-containing protein At1g66345, mitochondrial isoform X1 ... [more]
XP_022141356.15.9e-3584.04pentatricopeptide repeat-containing protein At1g66345, mitochondrial isoform X2 ... [more]
BLAST of Lag0005533 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q3ECH55.0e-2152.13Pentatricopeptide repeat-containing protein At1g66345, mitochondrial OS=Arabidop... [more]
Q9SAK05.8e-0935.44Pentatricopeptide repeat-containing protein At1g79490, mitochondrial OS=Arabidop... [more]
Q8S8P61.3e-0830.85Pentatricopeptide repeat-containing protein At2g32630 OS=Arabidopsis thaliana OX... [more]
Q9FIT71.7e-0832.98Pentatricopeptide repeat-containing protein At5g61990, mitochondrial OS=Arabidop... [more]
Q9FMF61.7e-0836.59Pentatricopeptide repeat-containing protein At5g64320, mitochondrial OS=Arabidop... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 47..76
e-value: 4.8E-6
score: 24.4
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 44..89
e-value: 3.9E-9
score: 36.6
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 12..41
e-value: 0.072
score: 13.4
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 44..78
score: 11.564229
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 9..43
score: 8.988323
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 1..98
e-value: 1.1E-18
score: 69.6
NoneNo IPR availablePANTHERPTHR45613:SF248PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN MITOCHONDRIALcoord: 1..94
NoneNo IPR availablePANTHERPTHR45613PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 1..94

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0005533.1Lag0005533.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000373 Group II intron splicing
biological_process GO:0032981 mitochondrial respiratory chain complex I assembly
biological_process GO:0000963 mitochondrial RNA processing
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005515 protein binding