Lag0004022 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004022
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionThioredoxin domain-containing protein
Locationchr6: 454968 .. 455881 (+)
RNA-Seq ExpressionLag0004022
SyntenyLag0004022
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATCATTCTTTTCAAGCGCTGTAGACGAAGGAGCCACAAGCCCTCATGTTCTGGTTTTCCAATCCCCCAACGACTGGGAAGCTCACTTCGACAACTCCAAGAAAACTAATAAACTGGTGCCTGATCTTTTTATTATTTTACAATCTGATCTTTTAATTTATACCCCTTTTTTTTTCTTGTAATTATATATATATATATATATATATATTTATCTTTTTGTTGGTTCATTGAAAGCTTGTGATAGATTTCAAAGCTTCTTGGTGCGGACCCTGTCAGTTTATGAAGCCCATATTCGCAGCCTTTGCTTCCAAGTACAAACATGTTGAATTCATTGAGATCGATGTCGATAAATTTAGTGTAAACCTCAGTCTCACTTACCTTACTATTATTCGTTTTATTTCTACGTTTTTAGATAATAGATATAAGGATTATTATGTTAAAGAGGTTAAATAACAAATTTAGTCAAAGCAATTTTAAAATATAGTTTATGATACTATTCATAGAGGACCCCGAATAGAGGAAACTTTAGAAAACTTAGGATGTAGATGACTAACAAACCAAATCGTAAGTCAACTCGAGTATGACTCAATTGGTTGAGTCATATATGCTTAATTAGCTAAGTGATAAAATGTTTGAATTCATCGGCCTCAAATGTTGTTGTTGAACTCGAAAAAAAAATCCCTTAAAGAAGTAATGAATAAAGTTTTACTTTTTTGGGTTTATAAACATACTCTTTTTAACCTATGCATTAAGTTAATTTCTTTTTACTAATTTGCAGGATGTGGCTAAAGAATTTGGGGTGACGCAGATGCCAACATTCCTACTAATTAAACAAGGGAAAGTGGTTGATAGTGTGGTTGGTGCAAAGAAGAATGAGCTCCAACAAGCAATTGAAACTCACACGGATTAA

mRNA sequence

ATGGGATCATTCTTTTCAAGCGCTGTAGACGAAGGAGCCACAAGCCCTCATGTTCTGGTTTTCCAATCCCCCAACGACTGGGAAGCTCACTTCGACAACTCCAAGAAAACTAATAAACTGCTTGTGATAGATTTCAAAGCTTCTTGGTGCGGACCCTGTCAGTTTATGAAGCCCATATTCGCAGCCTTTGCTTCCAAGTACAAACATGTTGAATTCATTGAGATCGATGTCGATAAATTTAGTGATGTGGCTAAAGAATTTGGGGTGACGCAGATGCCAACATTCCTACTAATTAAACAAGGGAAAGTGGTTGATAGTGTGGTTGGTGCAAAGAAGAATGAGCTCCAACAAGCAATTGAAACTCACACGGATTAA

Coding sequence (CDS)

ATGGGATCATTCTTTTCAAGCGCTGTAGACGAAGGAGCCACAAGCCCTCATGTTCTGGTTTTCCAATCCCCCAACGACTGGGAAGCTCACTTCGACAACTCCAAGAAAACTAATAAACTGCTTGTGATAGATTTCAAAGCTTCTTGGTGCGGACCCTGTCAGTTTATGAAGCCCATATTCGCAGCCTTTGCTTCCAAGTACAAACATGTTGAATTCATTGAGATCGATGTCGATAAATTTAGTGATGTGGCTAAAGAATTTGGGGTGACGCAGATGCCAACATTCCTACTAATTAAACAAGGGAAAGTGGTTGATAGTGTGGTTGGTGCAAAGAAGAATGAGCTCCAACAAGCAATTGAAACTCACACGGATTAA

Protein sequence

MGSFFSSAVDEGATSPHVLVFQSPNDWEAHFDNSKKTNKLLVIDFKASWCGPCQFMKPIFAAFASKYKHVEFIEIDVDKFSDVAKEFGVTQMPTFLLIKQGKVVDSVVGAKKNELQQAIETHTD
Homology
BLAST of Lag0004022 vs. NCBI nr
Match: KAB1220726.1 (Thioredoxin H2 [Morella rubra])

HSP 1 Score: 154.8 bits (390), Expect = 4.7e-34
Identity = 70/108 (64.81%), Postives = 88/108 (81.48%), Query Frame = 0

Query: 15  SPHVLVFQSPNDWEAHFDNSKKTNKLLVIDFKASWCGPCQFMKPIFAAFASKYKHVEFIE 74
           +P V+ F S + W+AHFD SK+TNKLLVIDF A+WCGPC+FM+P F  F++KY  V+FI+
Sbjct: 18  TPRVIAFHSKDQWKAHFDASKETNKLLVIDFTATWCGPCKFMEPAFKDFSAKYTDVDFIK 77

Query: 75  IDVDKFSDVAKEFGVTQMPTFLLIKQGKVVDSVVGAKKNELQQAIETH 123
           IDVD+   VA+EFGV  MPTFLLIK+GKVVD VVGA+K+ELQ+ IE H
Sbjct: 78  IDVDELQSVAREFGVEAMPTFLLIKKGKVVDKVVGARKDELQKKIEKH 125

BLAST of Lag0004022 vs. NCBI nr
Match: XP_020238958.1 (thioredoxin H3 [Cajanus cajan] >XP_020238959.1 thioredoxin H3 [Cajanus cajan] >KYP43005.1 Thioredoxin H-type 2 [Cajanus cajan])

HSP 1 Score: 149.8 bits (377), Expect = 1.5e-32
Identity = 67/105 (63.81%), Postives = 86/105 (81.90%), Query Frame = 0

Query: 18  VLVFQSPNDWEAHFDNSKKTNKLLVIDFKASWCGPCQFMKPIFAAFASKYKHVEFIEIDV 77
           +L F SP+ W+AHF+ SK+TNKL+VIDF A+WCGPC+FM+PI   FA+KY  VEFI+IDV
Sbjct: 24  ILAFHSPDKWKAHFEASKETNKLMVIDFTATWCGPCKFMEPIIEEFAAKYTEVEFIKIDV 83

Query: 78  DKFSDVAKEFGVTQMPTFLLIKQGKVVDSVVGAKKNELQQAIETH 123
           D+   V++EF V  MPTF+LIK+G+VVDSVVGA+K ELQ+ IE H
Sbjct: 84  DELKGVSQEFNVQAMPTFILIKKGEVVDSVVGARKEELQKLIEKH 128

BLAST of Lag0004022 vs. NCBI nr
Match: PHU27650.1 (Thioredoxin H2 [Capsicum chinense])

HSP 1 Score: 149.8 bits (377), Expect = 1.5e-32
Identity = 73/136 (53.68%), Postives = 94/136 (69.12%), Query Frame = 0

Query: 1   MGSFFSSAVDEG------------ATSPHVLVFQSPNDWEAHFDNSKKTNKLLVIDFKAS 60
           MGSF SS V  G            +    V+ F S N W+ HF+NSK+ NKL+V+DF AS
Sbjct: 1   MGSFLSSLVGGGEAQAATEESGSPSEESRVIAFHSSNRWQLHFNNSKQLNKLIVVDFAAS 60

Query: 61  WCGPCQFMKPIFAAFASKYKHVEFIEIDVDKFSDVAKEFGVTQMPTFLLIKQGKVVDSVV 120
           WCGPC+F++P   A ASKY  V+F++IDVD+ SDVA+EFGV  MPTFLL+KQGK V+ VV
Sbjct: 61  WCGPCKFIEPAIKAMASKYTDVDFVKIDVDELSDVAQEFGVQAMPTFLLLKQGKEVERVV 120

Query: 121 GAKKNELQQAIETHTD 125
           GAKK+EL++ I  H +
Sbjct: 121 GAKKDELEEKILKHRE 136

BLAST of Lag0004022 vs. NCBI nr
Match: XP_016561441.1 (PREDICTED: thioredoxin H2 [Capsicum annuum] >KAF3634196.1 Thioredoxin H2 [Capsicum annuum] >KAF3636953.1 Thioredoxin H2 [Capsicum annuum] >PHT91831.1 Thioredoxin H2 [Capsicum annuum])

HSP 1 Score: 149.4 bits (376), Expect = 2.0e-32
Identity = 75/136 (55.15%), Postives = 96/136 (70.59%), Query Frame = 0

Query: 1   MGSFFSS--------AVDEGATSPH----VLVFQSPNDWEAHFDNSKKTNKLLVIDFKAS 60
           MGSF SS        A  E + SP     V+ F S N W+ HF+NSK+ NKL+V+DF AS
Sbjct: 1   MGSFLSSMVGGVEAQAATEESGSPSEESCVIAFHSSNRWQLHFNNSKQLNKLIVVDFAAS 60

Query: 61  WCGPCQFMKPIFAAFASKYKHVEFIEIDVDKFSDVAKEFGVTQMPTFLLIKQGKVVDSVV 120
           WCGPC+F++P   A ASKY  V+F++IDVD+ SDVA+EFGV  MPTFLL+KQGK V+ VV
Sbjct: 61  WCGPCKFIEPAIKAMASKYTDVDFVKIDVDELSDVAQEFGVQAMPTFLLLKQGKEVERVV 120

Query: 121 GAKKNELQQAIETHTD 125
           GAKK+EL++ I  H +
Sbjct: 121 GAKKDELEEKILKHRE 136

BLAST of Lag0004022 vs. NCBI nr
Match: PHT57211.1 (Thioredoxin H2 [Capsicum baccatum])

HSP 1 Score: 149.4 bits (376), Expect = 2.0e-32
Identity = 75/136 (55.15%), Postives = 96/136 (70.59%), Query Frame = 0

Query: 1   MGSFFSS--------AVDEGATSP----HVLVFQSPNDWEAHFDNSKKTNKLLVIDFKAS 60
           MGSF SS        A  E + SP     V+ F S N W+ HF+NSK+ NKL+V+DF AS
Sbjct: 1   MGSFLSSLMGGGEAQAATEESGSPSEESRVIAFHSSNRWQLHFNNSKQLNKLIVVDFAAS 60

Query: 61  WCGPCQFMKPIFAAFASKYKHVEFIEIDVDKFSDVAKEFGVTQMPTFLLIKQGKVVDSVV 120
           WCGPC+F++P   A ASKY  V+F++IDVD+ SDVA+EFGV  MPTFLL+KQGK V+ VV
Sbjct: 61  WCGPCKFIEPAIKAMASKYTDVDFVKIDVDELSDVAQEFGVQAMPTFLLLKQGKEVERVV 120

Query: 121 GAKKNELQQAIETHTD 125
           GAKK+EL++ I  H +
Sbjct: 121 GAKKDELEKKILKHRE 136

BLAST of Lag0004022 vs. ExPASy Swiss-Prot
Match: Q38879 (Thioredoxin H2 OS=Arabidopsis thaliana OX=3702 GN=TRX2 PE=1 SV=2)

HSP 1 Score: 127.9 bits (320), Expect = 8.1e-29
Identity = 53/102 (51.96%), Postives = 78/102 (76.47%), Query Frame = 0

Query: 18  VLVFQSPNDWEAHFDNSKKTNKLLVIDFKASWCGPCQFMKPIFAAFASKYKHVEFIEIDV 77
           VL F S   W+ HF+  K++NKLLV+DF ASWCGPC+ ++P   A A K+  V+F+++DV
Sbjct: 27  VLKFSSSARWQLHFNEIKESNKLLVVDFSASWCGPCRMIEPAIHAMADKFNDVDFVKLDV 86

Query: 78  DKFSDVAKEFGVTQMPTFLLIKQGKVVDSVVGAKKNELQQAI 120
           D+  DVAKEF VT MPTF+L+K+GK ++ ++GAKK+EL++ +
Sbjct: 87  DELPDVAKEFNVTAMPTFVLVKRGKEIERIIGAKKDELEKKV 128

BLAST of Lag0004022 vs. ExPASy Swiss-Prot
Match: P29449 (Thioredoxin H-type 1 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 3.2e-25
Identity = 56/117 (47.86%), Postives = 73/117 (62.39%), Query Frame = 0

Query: 8   AVDEGATSPHVLVF--QSPNDWEAHFDNSKKTNKLLVIDFKASWCGPCQFMKPIFAAFAS 67
           A ++  +S    VF      +W  +F    +T KL+V+DF ASWCGPC+F+ PI A  A 
Sbjct: 2   AANDATSSEEGQVFGCHKVEEWNEYFKKGVETKKLVVVDFTASWCGPCRFIAPILADIAK 61

Query: 68  KYKHVEFIEIDVDKFSDVAKEFGVTQMPTFLLIKQGKVVDSVVGAKKNELQQAIETH 123
           K  HV F+++DVD+   V+ E+ V  MPTF+ IK GK VD VVGAKK ELQQ I  H
Sbjct: 62  KMPHVIFLKVDVDELKTVSAEWSVEAMPTFVFIKDGKEVDRVVGAKKEELQQTIVKH 118

BLAST of Lag0004022 vs. ExPASy Swiss-Prot
Match: Q851R5 (Thioredoxin H2-2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0800700 PE=2 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 6.0e-24
Identity = 60/128 (46.88%), Postives = 81/128 (63.28%), Query Frame = 0

Query: 1   MGSFFSS------AVDEGATSPHVLVFQS---PNDWEAHFDNSKKTNKLLVIDFKASWCG 60
           MGSFFS+      A D+G  S  V V  +      W AH  N    NKL+VIDF A+WCG
Sbjct: 1   MGSFFSTMFTPPPAADDGGDSRVVAVHSTATWDEQWGAHKSN---PNKLIVIDFSATWCG 60

Query: 61  PCQFMKPIFAAFASKYKHVEFIEIDVDKFSDVAKEFGVTQMPTFLLIKQGKVVDSVVGAK 120
           PC+F++P F   A ++    F +IDVD+ S+VA+++ V  MPTF+LIK GK V  VVGAK
Sbjct: 61  PCRFIEPAFKDMAGRFADAVFFKIDVDELSEVARQWKVEAMPTFVLIKGGKEVSRVVGAK 120

BLAST of Lag0004022 vs. ExPASy Swiss-Prot
Match: Q39362 (Thioredoxin H-type 2 OS=Brassica napus OX=3708 GN=THL-2 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 7.9e-24
Identity = 51/98 (52.04%), Postives = 67/98 (68.37%), Query Frame = 0

Query: 27  WEAHFDNSKKTNKLLVIDFKASWCGPCQFMKPIFAAFASKY-KHVEFIEIDVDKFSDVAK 86
           W    D +K++NKL+VIDF ASWC PC+ + P+FA  A K+     F ++DVD+  +VA+
Sbjct: 17  WAVQLDTAKQSNKLIVIDFTASWCPPCRMIAPVFADLAKKFMSSAIFFKVDVDELQNVAQ 76

Query: 87  EFGVTQMPTFLLIKQGKVVDSVVGAKKNELQQAIETHT 124
           EFGV  MPTF+LIK G VVD VVGA+K +L   I  HT
Sbjct: 77  EFGVEAMPTFVLIKDGNVVDKVVGARKEDLHATIAKHT 114

BLAST of Lag0004022 vs. ExPASy Swiss-Prot
Match: Q0D840 (Thioredoxin H1 OS=Oryza sativa subsp. japonica OX=39947 GN=TRXH PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 1.8e-23
Identity = 48/115 (41.74%), Postives = 76/115 (66.09%), Query Frame = 0

Query: 8   AVDEGATSPHVLVFQSPNDWEAHFDNSKKTNKLLVIDFKASWCGPCQFMKPIFAAFASKY 67
           A +EG     V+   + ++++A    +K+  K+++IDF ASWCGPC+F+ P+FA +A K+
Sbjct: 2   AAEEGV----VIACHNKDEFDAQMTKAKEAGKVVIIDFTASWCGPCRFIAPVFAEYAKKF 61

Query: 68  KHVEFIEIDVDKFSDVAKEFGVTQMPTFLLIKQGKVVDSVVGAKKNELQQAIETH 123
               F+++DVD+  +VA+++ V  MPTFL IK G   D VVGA+K++LQ  I  H
Sbjct: 62  PGAVFLKVDVDELKEVAEKYNVEAMPTFLFIKDGAEADKVVGARKDDLQNTIVKH 112

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAB1220726.14.7e-3464.81Thioredoxin H2 [Morella rubra][more]
XP_020238958.11.5e-3263.81thioredoxin H3 [Cajanus cajan] >XP_020238959.1 thioredoxin H3 [Cajanus cajan] >K... [more]
PHU27650.11.5e-3253.68Thioredoxin H2 [Capsicum chinense][more]
XP_016561441.12.0e-3255.15PREDICTED: thioredoxin H2 [Capsicum annuum] >KAF3634196.1 Thioredoxin H2 [Capsic... [more]
PHT57211.12.0e-3255.15Thioredoxin H2 [Capsicum baccatum][more]
Match NameE-valueIdentityDescription
Q388798.1e-2951.96Thioredoxin H2 OS=Arabidopsis thaliana OX=3702 GN=TRX2 PE=1 SV=2[more]
P294493.2e-2547.86Thioredoxin H-type 1 OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q851R56.0e-2446.88Thioredoxin H2-2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0800700 PE=2 S... [more]
Q393627.9e-2452.04Thioredoxin H-type 2 OS=Brassica napus OX=3708 GN=THL-2 PE=2 SV=1[more]
Q0D8401.8e-2341.74Thioredoxin H1 OS=Oryza sativa subsp. japonica OX=39947 GN=TRXH PE=1 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00421THIOREDOXINcoord: 49..58
score: 56.0
coord: 41..49
score: 44.26
coord: 88..99
score: 37.96
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 6..124
e-value: 9.1E-37
score: 127.7
NoneNo IPR availablePANTHERPTHR10438THIOREDOXINcoord: 12..123
NoneNo IPR availablePANTHERPTHR10438:SF413THIOREDOXIN H2coord: 12..123
NoneNo IPR availableCDDcd02947TRX_familycoord: 29..120
e-value: 1.15658E-34
score: 113.422
IPR013766Thioredoxin domainPFAMPF00085Thioredoxincoord: 30..120
e-value: 1.8E-26
score: 92.1
IPR013766Thioredoxin domainPROSITEPS51352THIOREDOXIN_2coord: 9..124
score: 14.02408
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 13..122

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004022.1Lag0004022.1mRNA