Cucsat.G8367 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G8367
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Neutral ceramidase
Location	ctg1557: 448655 .. 454284 (-)
RNA-Seq Expression	Cucsat.G8367
Synteny	Cucsat.G8367

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CCGGGAGCTAAATTATCTTAAAAATATAAACTGTGCAAGTATTATTTTGACTAAAGCATTCATCCAACATTTCTAGGTATGGGGATTTATACACTGAAAAGAATGTTGCTATTAGTGGAATCCATAGTCATGCTGGGCCAGGTGGTTATCTCCAGTATGTTGTGTATATCGTGACATCCCTTGGATTTGTGCGCCAGTCATTTAATGTACTTGTGGATGGTATCGAGAAGAGCATCATACAAGCTCATGAAAATCTCTCGCCAGGATCAATTTTAATAAATAAGGGTAATAATGGATTAAAACACTCTCTCTTGGTGCATGAAATTTATCTTTTCTTGAACTTTTCCGTTTGTTGAGGTTAAATTTTCACTTCAGTTTATCCATATCCTCAACAATCATACAAATGAGCTTAAATCAGAAATTCTTTTTGCATAGTCTGTTGGCTGTTGTGAAGTGTGGCTTCCTAACGACTGCATTAAAGTATTTGGGTGTTAGTTCAGTTCCCTACTATAGATAGATTCTATATCTCTTTCTTGATCTTGCAAAAATTTGTATTTTTAATGAAAAGGTTTGTTTAGGTCACAATCATCACTTGTATGATCATCGGCATGTACTTGTCAACTTGTGAATCTGATTATTCCAAGTGAGAGCATAGATTCACGCTTATAATGATTAGTAAACAACTTCAGCTGTTGGAATACTTAGAAACTTGTTACTTAAACCTTTAACCTTTTGGAATATAATTAATACTCTTACAGGAGAACTCATTGATGCTGGTGTGAACCGCAGCCCGAGTGCTTACCTAAATAATCCTGCCTCTGAGCGTAGCAAATACAAATATGACGTTGACAAAGAAATGACACTTTTAAAGTTCATAGATGATGAGTGGGGTCCTGTTGGTACCTTCAATTGGTTTGCAACTCATGGAACTTCTATGAGTCGTACAAACGCACTAATAAGTGGCGATAACAAGGGTGCTGCTGCAAGATTTATGGAAGATTGGTTTAAGCAAAAGGGTACTGGAACCTTGCACCATGGTGAATCTGAAGCTGACAGTATTCCACGCAGAGTTTCAAACATTGTTCCTGAAGTATATAAAGACAGTAAGTTACTAAGTTCTTAATGTTAGAAAATAAATCTATTTAGAGATGTTTAAATGCTCAGTTGCTCATCAAATTTCATATCTTAGGAAAGCATGTCTTTCTTATTCAAGTGACTTGTCACTTAATATTCTTTATTAGTTCTATTTATCTTGGAATCAGGCACAGTGCAATCCAAATTTATCATGTTCAATTTTTGGAATTATGTCTCTCATGTACTTTAACTTTCAGGGCTATGTCTAACAGATACTTCTTAAACATATCCCTCAAAGTTTAGGAATCAAACTTGTAAATTTACTGGTGTCCATGAAAGTTACATTTTATTGAATATACTATGTGCTATAAATTGTCTGTAGACAAGTTTTCTTATCCCGATAATATCACATTTCAGAGCAGGAGTTGTTGGAACTTGCAGCATCGTTTCAGTCACAACCAGGTAGACCAGCAACCAGGGTTCTAAGCATTTCAAGCCGTGTTAGAAATGTTCTCAGGCAGGCTGATAGGCCGCAGTTTGTATCTGCTTTTTGTCAATCAAACTGTGGTGATGTTAGCCCAAATACTCTTGGGGCATTCTGCCTAGACACTGGACTGCCATGTGACTTTAACCACAGTACTTGCGGGGGAAAGAATGAGCTATGTTATGGACGTGGACCGGGGTATATTTCTCTCTGCATTGAAGATTCTTTTTTTTCTTTTCTTCAAGAATTACTGATCATTTGCGTACGATGTGAACCAAGTTGTCATGTACTTTAACTTACAGCTACCCTGATGAATTTGAAAGTACACGTATTATTGGTGAGAAGCAATTCAGAAAAGCTGTAGATTTATTCAGTAAAGCGTCGGAACAGCTCACAGGAAAAGTGGACTTTCGTCACTCTTATGTAGACTTCTCCCACCTTGAAGTGTCTCTTAATAAACAGGGAGGAGTCACTGAGGTGGTAAAAACATGTCCTGCTGCTATGGGGTTTGCATTTGCAGCTGGGACAACTGATGGACCTGGAGCATTTGATTTTAAGCAAGGAGATGACAAGGTTTCTCTTATTCATGTGCTTATTAACTTTTGTTAGAAAGCAGTTCTTCAGTTTCAGTAGAGTGCATGTTTTCATTTTTTTCACTGCCAATGGTTGGTCCCAATTTCCATGACATTTCCTTTTGTTCTTTTCTCATTATTCAAGGGCAATGCTTTTTGGAAGTTGGTACGCAATGTACTCAAAGCACCAGGCAACGAACAAATTAGTTGTCAAAGTCCCAAGCCGATTCTACTTGACACCGGCGAGATGAAGACACCATATGACTGGGCTGTAAGTGGTTGCACATAATCTAGTGTATTGTTTTTATTTTTTGACATATTCCAAAGACTACATCTTTTGGGCAAATGGTAAAAACCACCCCTAGTTGCAATTATACCCTCAAATTTTCAATTGTAAAAACTGAGGTCTCAAACTTATAAAAGTGTTAAAATTGAACCCTCAAACTTATATAGTTGTAGAAATTATAAAATTGTATAAGCTTAAGAGTTCATTTTTTATCAGTTGTGGATCGCTGTTGTAAGTTTGATGGTTCAATTTAGTTTGGGGGCTTTACAATTGAAAGTTTTAGAGTGTAATTGTGACTGCAGTGTATTTTTTGCAATTTGCCCCATTAATTCATCAGATAAAGGTTATTTTGATAAATTTTATGTTAGTGTATTGATGGGAAAATATATTGGAAATGTAATCAGAATATGAAGTGTGACAAATTGGTAATTGCTTGGTAAAAGAGGAATTCAGGTAGATTTGGATAGAAATCAGTCCTTAGTAGGTGGATTCTAGGGAGAGAATCCTCTTGAAAGATTTTTGATATTGTAATTTCTCTTTGATATTACAATTTAGGTACAGGATTGTTTTCTTCTAATCGAGATCCTAACATTTTCCTTAAAACTTTCTTTCTCTAATAACTTTTCAGCCCTCAATACTTCCAATTCAAATTCTCCGCATTGGGCAGCTTGTCATTCTTGATGTACCAGGAGGTAGTACCTTCCCTTGTTATTTATACATATCTATCTATATTTGTAGCTGGGTTAATCCTAATGACAACTAATTATCTTATAGTTTAGTTCCTTTTATAAATAATGTAAATTGGGTAAATGAGAGACTAAATCCACTTTGTGTAATTTAGTGGGCAGTGACTCTTAAGTCAGACTAAATTCCATTTAGTATAATTTAATAGGGGCACTAACTCTTAAAGTTGGACTAAATCCACTTTGTGTAACATAATGGGCTCTGGCTCTCATAGTATTAGAGGGGTGAGGTTGTTGACAAACTCATGATTACTAGTCCATTTAGACTTTTTGAGATAACCTCAGTTGGAGATAAAGTTAGGTGGGACTTCAATGTATGAAATTCAGTTGTAAATTATTGAAGAGAAGACAAAATATACTGGGATGATAGAAGCTGCTCTTATTATTTTCTTCAATAATCAAAAGAGTACAAGGGGAATATATATAGGCTATAATAAGCCCTAACAAAAAAAAAGAAATAAAACTAGGTTACAATAAATTACAAAAACTCAAAAGCAGCAATTTATTGCTTTTTCCAAGAACCATTATAAATCAAATCAAAACATGAATTTTGACTGTTTGAGGGAGCAGTTTTGGTCCTGGCAAAACCACCGCCGATCATAGATTTATTGGCTTCAAATAAACTAGAATAATAAATGTTGCTTCCGTTTGCTATGAGTTCGGAATGGAAAGTTCAAAATGATCACTGTCTCTTAATTTGAGTTACATTTCCATGAGCTATAAATATATTCATTTTTTTTAATAGAAAATATTGATAGCTCATGTACCTTCTTTCCCTCTGCAGATTCTCCTAGAAATTTCCCTTCTTAAATTTAGTTTAATATGGTTAATTTTATTAAAAAACCTTCACAGAGTTCACGACAATGGCGGGGAGACGGCTTCGGGATGCTGTAAAAACAGTGTTAACTACTGGAGCCAAGAAAGAATTCAATAGTAATGTTCATGTTGTCATAGCAGGCTTGACTAATACCTATTCTCAATATGTCACCACCTTTGAAGAATATAAGATGCAGAGATATGAGGTTAGCGAATTGGTCTTTCAGCTTTTTGTGTACCTATCATCAATTTTTAATGTCTTATTTCTTACTTTTAAGACTTTCTAGTTTATTTCATTTTGGCCCATGAATTTTCAAAATATCTATTTTAATATAAAAACTTAAAAGTGAAAAAAAATTCATTTTCTTCCTTGCTGTTATTCTGCTGTCAATTCTTCTACCTCACGGCTCACAGAATACCATCTTCAACTACCTGTATGACTTCAATAGATTTCATGTCAGCATGCTAAGACACATGTTCAAAGCTATATTATGTGTATGTTGGTTGGATTATGAAAATGCTTTTAATCATTTGAAGTTTGGTAGTGATTCACTCCGAAAATGCTTTTAATCATTCAAAATCAATTTTAATAGTATGAAAAATGTATTTGAAAGTGTAAAATTAAACATTAAATTGACTTTGAGTGATTAGAAGTCTAATTTAGAATGACTTTAAACACAATTTTAACCATTTCAAAATATGCTAGGAAATTCTTTAGTAAGCCGTTTGAATGTTTAGGGCTTAAATTCGAATAAGCTTAGAAGTTAATTGAAATAAAATAAGATTGATATCCTTACTTTCATTTGACATTGTTCTTTGATCTCAGGGTGCCTCCACGTTGTATGGTCCACACACATTGGAGGCCTACATACAAGAGTTCAAGAAGCTTGCTCAATCTCTCATAGATGGCACGCCTGTTGCGCCAGGGCCACAGCCCCCTGATCTCCTTGCTAGGCAAATAAGCCTGCTTCCACCGGTTATCCTGGATATGACTCCATTAGGAGTGAGCTTCGGGGACGTAAAATTCGATGTACCCTCTAACTCTTCCTTCAAGAGAGGTAACTTGGTGAAAGTAACGTTTTGGACTGGATGCCCTCGAAATGATCTCATGACTGAAGGAACATTTGCTCTCGTCGAAATCCTTCAGAAGAATACTTGGGTTCCAGCTTACGACGATGACGACTTTTGCTTGCGCTTTAAGTGGTCGAGACCTGCTCCTCTTAGTGCTCAAAGTTATGCAACCATAGAGTGGAGAATACCACAAACCGCAGTGTCAGGTGTCTACAGAATCAGACATTTTGGAGCAGCAAAAAGTCTCCTTGGGTCGATTCGACATTTTACGGGTTCTTCTAGTGCATTTGTTGTAGCTTGAGAACATATCAAAATTATTTATGGTGGTAGGGAGGGATGTTGATTGTCAGTTGCTTTATTTATTTCACCAGTTAAAAAGCTAATTTTTTCTTCAAGTAATTGGGTAGTAGATGTACTAGGATAACCTAATTAGAATGGCATTATGTACAATAGTGCCCATCTTATTGTGATATTAAAATGATAAAAGAGAAGTGAACAAAATTCTAAAAAGTCTTCAAATATAATAATTCAAACTCATGTTCCTATAAGCCCATCGAGATCAACAAGACAATTACCTTCTAATG

Coding sequence (CDS)

ATGACACTTTTAAAGTTCATAGATGATGAGTGGGGTCCTGTTGGTACCTTCAATTGGTTTGCAACTCATGGAACTTCTATGAGTCGTACAAACGCACTAATAAGTGGCGATAACAAGGGTGCTGCTGCAAGATTTATGGAAGATTGGTTTAAGCAAAAGGGTACTGGAACCTTGCACCATGGTGAATCTGAAGCTGACAGTATTCCACGCAGAGTTTCAAACATTGTTCCTGAAGTATATAAAGACAAGCAGGAGTTGTTGGAACTTGCAGCATCGTTTCAGTCACAACCAGGTAGACCAGCAACCAGGGTTCTAAGCATTTCAAGCCGTGTTAGAAATGTTCTCAGGCAGGCTGATAGGCCGCAGTTTGTATCTGCTTTTTGTCAATCAAACTGTGGTGATGTTAGCCCAAATACTCTTGGGGCATTCTGCCTAGACACTGGACTGCCATGTGACTTTAACCACAGTACTTGCGGGGGAAAGAATGAGCTATGTTATGGACGTGGACCGGGCTACCCTGATGAATTTGAAAGTACACGTATTATTGGTGAGAAGCAATTCAGAAAAGCTGTAGATTTATTCAGTAAAGCGTCGGAACAGCTCACAGGAAAAGTGGACTTTCGTCACTCTTATGTAGACTTCTCCCACCTTGAAGTGTCTCTTAATAAACAGGGAGGAGTCACTGAGGTGGTAAAAACATGTCCTGCTGCTATGGGGTTTGCATTTGCAGCTGGGACAACTGATGGACCTGGAGCATTTGATTTTAAGCAAGGAGATGACAAGGGCAATGCTTTTTGGAAGTTGGTACGCAATGTACTCAAAGCACCAGGCAACGAACAAATTAGTTGTCAAAGTCCCAAGCCGATTCTACTTGACACCGGCGAGATGAAGACACCATATGACTGGGCTCCCTCAATACTTCCAATTCAAATTCTCCGCATTGGGCAGCTTGTCATTCTTGATGTACCAGGAGAGTTCACGACAATGGCGGGGAGACGGCTTCGGGATGCTGTAAAAACAGTGTTAACTACTGGAGCCAAGAAAGAATTCAATAGTAATGTTCATGTTGTCATAGCAGGCTTGACTAATACCTATTCTCAATATGTCACCACCTTTGAAGAATATAAGATGCAGAGATATGAGGGTGCCTCCACGTTGTATGGTCCACACACATTGGAGGCCTACATACAAGAGTTCAAGAAGCTTGCTCAATCTCTCATAGATGGCACGCCTGTTGCGCCAGGGCCACAGCCCCCTGATCTCCTTGCTAGGCAAATAAGCCTGCTTCCACCGGTTATCCTGGATATGACTCCATTAGGAGTGAGCTTCGGGGACGTAAAATTCGATGTACCCTCTAACTCTTCCTTCAAGAGAGGTAACTTGGTGAAAGTAACGTTTTGGACTGGATGCCCTCGAAATGATCTCATGACTGAAGGAACATTTGCTCTCGTCGAAATCCTTCAGAAGAATACTTGGGTTCCAGCTTACGACGATGACGACTTTTGCTTGCGCTTTAAGTGGTCGAGACCTGCTCCTCTTAGTGCTCAAAGTTATGCAACCATAGAGTGGAGAATACCACAAACCGCAGTGTCAGGTGTCTACAGAATCAGACATTTTGGAGCAGCAAAAAGTCTCCTTGGGTCGATTCGACATTTTACGGGTTCTTCTAGTGCATTTGTTGTAGCTTGA

Protein sequence

MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTGSSSAFVVA

Homology

[Close]

BLAST of Cucsat.G8367 vs. ExPASy Swiss-Prot
Match: F4HQM3 (Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1)

HSP 1 Score: 845.1 bits (2182), Expect = 4.5e-244
Identity = 406/564 (71.99%), Postives = 463/564 (82.09%), Query Frame = 0


Query: 1   MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHH 60
           MTLLKF+DD+WGPVG+FNWFATHGTSMSRTN+LISGDNKGAA+RFMEDW++Q      + 
Sbjct: 215 MTLLKFVDDQWGPVGSFNWFATHGTSMSRTNSLISGDNKGAASRFMEDWYEQNTAERSYS 274

Query: 61  GESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADR 120
            E  +D IPRRVS+++        ELLELA+ F+SQPG+P TR+ S + RVR+ LR+AD+
Sbjct: 275 EEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTRISSSARRVRSALRKADK 334

Query: 121 PQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 180
           P FVSAFCQ+NCGDVSPN LGAFCLDTGLPCDFNHSTCGGKNE+CYGRGPGYPDEFESTR
Sbjct: 335 PGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEMCYGRGPGYPDEFESTR 394

Query: 181 IIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGF 240
           IIGE+QF+ A++LF+KASEQL GKVD+RH YVDFS L V+L K+ G +EVVKTCPAAMGF
Sbjct: 395 IIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPAAMGF 454

Query: 241 AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPY 300
           AFAAGTTDGPGAFDF QGDDKGN FW+LVRNVLK P  +QI C  PKPILLDTGEM  PY
Sbjct: 455 AFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEMTKPY 514

Query: 301 DWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAG 360
           DWAPSIL +Q+LRIGQL IL VPGEFTTMAGRRLR AVKT L     K+ +  +HVVIAG
Sbjct: 515 DWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKNSGNKDLSGEIHVVIAG 574

Query: 361 LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPD 420
           L N YSQYVTTFEEY++QRYEGASTLYGPHTL  YIQEFKKL++SL+   PV PGPQPPD
Sbjct: 575 LANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSKSLVLDMPVQPGPQPPD 634

Query: 421 LLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGN-LVKVTFWTGCPRNDLMTEG 480
           LL +Q+S L PV++D TP G SFGDV  DVP N S KRGN  V V F + CPRNDL+TEG
Sbjct: 635 LLDKQLSFLTPVMMDTTPSGDSFGDVISDVPKNLSLKRGNGQVTVVFRSACPRNDLLTEG 694

Query: 481 TFALVEILQK--NTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIR 540
           TF LVE L++   TW P YDDDD CLRFKWSR   LS++S AT+EWRIP++A  GVYRI 
Sbjct: 695 TFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQATVEWRIPESASPGVYRIT 754

Query: 541 HFGAAKSLLGSIRHFTGSSSAFVV 562
           HFGAAK L GS+ HFTGSSSAFVV
Sbjct: 755 HFGAAKKLFGSVHHFTGSSSAFVV 778

[Close]

BLAST of Cucsat.G8367 vs. ExPASy Swiss-Prot
Match: Q304B9 (Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 1.4e-240
Identity = 394/562 (70.11%), Postives = 472/562 (83.99%), Query Frame = 0


Query: 1   MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHH 60
           MTL+KF+D + GP G+FNWFATHGTSMSRTN+LISGDNKGAAARFMEDWF+     ++  
Sbjct: 204 MTLVKFVDSQLGPTGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSV-- 263

Query: 61  GESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADR 120
               + +IPRRVS IV +  +++  LL++AA+++S  G    + L + +RVRN      +
Sbjct: 264 ---SSRNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGHSVDKSLDVKTRVRN----GSK 323

Query: 121 PQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 180
            +FVSAFCQSNCGDVSPNTLG FC+DTGLPCDFNHSTC G+NELCYGRGPGYPDEFESTR
Sbjct: 324 RKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTR 383

Query: 181 IIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGF 240
           IIGEKQF+ AV+LF+KA+E+L GK+ ++H+Y+DFS+L+V++ K GG +E VKTCPAAMGF
Sbjct: 384 IIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGF 443

Query: 241 AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPY 300
            FAAGTTDGPGAFDFKQGDD+GN FW+LVRNVL+ PG EQ+ CQ PKPILLDTGEMK PY
Sbjct: 444 GFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPY 503

Query: 301 DWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAG 360
           DWAPSILPIQILRIGQLVIL VPGEFTTMAGRRLRDA+K+ L +   KEF++N+HVVIAG
Sbjct: 504 DWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAG 563

Query: 361 LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPD 420
           LTNTYSQY+ TFEEY++QRYEGASTLYG HTL AYIQEFKKLA +L++G  +  GPQPPD
Sbjct: 564 LTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTLPRGPQPPD 623

Query: 421 LLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGT 480
           LL +QISLL PV++D TPLGV FGDVK DVP  S+F+RG  V  TFW+GCPRNDLMTEG+
Sbjct: 624 LLDKQISLLSPVVVDSTPLGVKFGDVKADVPPKSTFRRGQQVNATFWSGCPRNDLMTEGS 683

Query: 481 FALVEILQK-NTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHF 540
           FA+VE L++   W P YDDDDF L+FKWSRPA LS++S ATIEWR+P++AV+GVYRIRH+
Sbjct: 684 FAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHY 743

Query: 541 GAAKSLLGSIRHFTGSSSAFVV 562
           GA+KSL GSI  F+GSSSAFVV
Sbjct: 744 GASKSLFGSISSFSGSSSAFVV 756

[Close]

BLAST of Cucsat.G8367 vs. ExPASy Swiss-Prot
Match: Q0JL46 (Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1 SV=1)

HSP 1 Score: 807.0 bits (2083), Expect = 1.4e-232
Identity = 385/570 (67.54%), Postives = 462/570 (81.05%), Query Frame = 0


Query: 1   MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKG--TGTL 60
           MTL+KF+DDE GPVG+FNWFATHGTSMSRTN+LISGDNKGAAARFMEDW +Q G    + 
Sbjct: 219 MTLIKFVDDELGPVGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWAEQMGLPKQSA 278

Query: 61  HHGESEADS------IPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVR 120
           H    +  S      +PRRVS I+PE  +   +L++LA+S+++  GR      SI+ R+R
Sbjct: 279 HANSDDLRSLHKTSVLPRRVSTIIPEPNEITDDLIQLASSYEASGGRRLAG-SSITRRIR 338

Query: 121 NVLRQADRPQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGY 180
           +   Q ++P+FVSAFCQSNCGDVSPN LG FC+DT LPCDFNHSTC GKNELCYGRGPGY
Sbjct: 339 ST--QQNKPKFVSAFCQSNCGDVSPNVLGTFCIDTNLPCDFNHSTCNGKNELCYGRGPGY 398

Query: 181 PDEFESTRIIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVK 240
           PDEFESTR+IG +QF KA DLF  ASE++ GK+D+RH+Y+DFS LEV ++   G  + VK
Sbjct: 399 PDEFESTRVIGNRQFLKARDLFDSASEEIQGKIDYRHTYLDFSKLEVKVSTSAGGQQTVK 458

Query: 241 TCPAAMGFAFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLD 300
           TCPAAMGFAFAAGTTDGPGAFDF+QGD KGN FWKLVRN+LK PG +Q+ C SPKPILLD
Sbjct: 459 TCPAAMGFAFAAGTTDGPGAFDFRQGDVKGNPFWKLVRNLLKTPGKDQVECHSPKPILLD 518

Query: 301 TGEMKTPYDWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNS 360
           TGEMK PYDWAP+ILP+Q++RIGQLVIL VPGEFTTMAGRRLRDAVKTVLT+G   EF+ 
Sbjct: 519 TGEMKEPYDWAPAILPVQMIRIGQLVILCVPGEFTTMAGRRLRDAVKTVLTSG-NSEFDK 578

Query: 361 NVHVVIAGLTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPV 420
           N+HVV+AGLTN+YSQY+TTFEEY++QRYEGASTLYGPHTL AYIQEF+KLA ++I    V
Sbjct: 579 NIHVVLAGLTNSYSQYITTFEEYQIQRYEGASTLYGPHTLSAYIQEFQKLAMAMIANKEV 638

Query: 421 APGPQPPDLLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPR 480
               QPPD+L +QI LLP V+ D TPLGV FGDV  DVP NS+F +G+ V  TF++ CPR
Sbjct: 639 PTNFQPPDMLDKQIGLLPGVVFDSTPLGVKFGDVNSDVPGNSTFNKGSTVNATFYSACPR 698

Query: 481 NDLMTEGTFALVEILQ-KNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVS 540
           NDL+T+GTFALVE L   N WVP YDDDD+ LRFKWSRPA LS++S+AT+EW +P+ A +
Sbjct: 699 NDLLTDGTFALVEKLDGNNNWVPVYDDDDWSLRFKWSRPARLSSRSFATLEWTVPEDAAA 758

Query: 541 GVYRIRHFGAAKSLLGSIRHFTGSSSAFVV 562
           GVYR+RHFGA+K + GS+RHFTG+S AF V
Sbjct: 759 GVYRLRHFGASKPMFGSVRHFTGTSRAFAV 784

[Close]

BLAST of Cucsat.G8367 vs. ExPASy Swiss-Prot
Match: F4KHQ8 (Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 1.8e-216
Identity = 377/565 (66.73%), Postives = 440/565 (77.88%), Query Frame = 0


Query: 1   MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHH 60
           MTL+KF+DD+WGPV                            AR MEDWF+++       
Sbjct: 207 MTLVKFVDDQWGPV----------------------------ARIMEDWFERENGCRSVD 266

Query: 61  GESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADR 120
            ES     PRRVS+I+ + Y   Q+L+E+A+S  S  G+  TR+ S++ RVR+  R AD+
Sbjct: 267 VES-----PRRVSSIISDPY--DQDLMEMASSLLSTGGKTVTRMSSVARRVRSRFRHADK 326

Query: 121 PQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 180
           P+FVSAFCQ+NCGDVSPN LGAFC+DTGLPC+FN STCGGKNE CYGRGPGYPDEFESTR
Sbjct: 327 PRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNEQCYGRGPGYPDEFESTR 386

Query: 181 IIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGF 240
           IIGE+QF+KA DLF+KASE++ GKVD+RH+YVDFS LEV++N Q G +EVVKTCPAAMGF
Sbjct: 387 IIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTINGQNGGSEVVKTCPAAMGF 446

Query: 241 AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPY 300
            FAAGTTDGPGAFDFKQGDD+GN FW+LVRN+LK P  EQ+ CQ PKPILLDTGEMK PY
Sbjct: 447 GFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRCQRPKPILLDTGEMKQPY 506

Query: 301 DWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAK-KEFNSNVHVVIA 360
           DWAPSILP+QILRIGQLVIL VPGEFTTMAGRRLRDAVKTVL  G+  +EF+    VVIA
Sbjct: 507 DWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLKEGSNGREFS----VVIA 566

Query: 361 GLTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPP 420
           GLTN+YSQY+ TFEEY++QRYEGASTLYGPHTL  YIQEFKKLA  L+      PGPQPP
Sbjct: 567 GLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLANDLLSAQTTDPGPQPP 626

Query: 421 DLLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRG-NLVKVTFWTGCPRNDLMTE 480
           DLL +QISLL PV+ DMTP+G +FGDV  DVP  S F++G ++V+V F +  PRNDLMTE
Sbjct: 627 DLLHKQISLLTPVVADMTPIGTAFGDVTSDVPRLSKFRKGADIVRVQFRSANPRNDLMTE 686

Query: 481 GTFALVE--ILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRI 540
           GTFALVE  +  + TWVP YDDDDFCLRFKWSRP  LS QS ATIEWRIP+TA  GVYRI
Sbjct: 687 GTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQSTATIEWRIPETASPGVYRI 732

Query: 541 RHFGAAKSLLGSIRHFTGSSSAFVV 562
            HFG+AK+ + SI HF+GSSSAFVV
Sbjct: 747 THFGSAKTPISSIHHFSGSSSAFVV 732

[Close]

BLAST of Cucsat.G8367 vs. ExPASy Swiss-Prot
Match: Q55G11 (Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 4.7e-116
Identity = 249/568 (43.84%), Postives = 318/568 (55.99%), Query Frame = 0


Query: 1   MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQ-KGTGTLH 60
           MT+L+  D    P    ++FA H TSM+ TN LISGDNKG A+     W KQ  G GT  
Sbjct: 225 MTVLRIEDMNGNPFAAISFFAVHCTSMNNTNHLISGDNKGYASYL---WEKQVNGPGT-- 284

Query: 61  HGESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQAD 120
                                                                     A 
Sbjct: 285 ----------------------------------------------------------AG 344

Query: 121 RPQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYP-DEFES 180
           +  FV+AF QSN GDVSPNT G  C D G PCD+  STC G+NE C+  GPG   D FES
Sbjct: 345 KGPFVAAFGQSNEGDVSPNTRGPTCRD-GSPCDYKTSTCNGRNEECWSLGPGKDGDMFES 404

Query: 181 TRIIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAM 240
           T+IIG  QF KA++LF+ AS Q++G V +RHS+V F+++ V      GV     TC  AM
Sbjct: 405 TQIIGGNQFNKALELFNNASIQVSGPVQYRHSWVQFTNVSVEPPYNSGVDNAT-TCRGAM 464

Query: 241 GFAFAAGTTDGPGAFDFKQGDD--KGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEM 300
           G++FAAGTTDGPGAF+F Q D+   GN FW  + + +  P  +QI CQSPKPILLD G M
Sbjct: 465 GYSFAAGTTDGPGAFNFVQSDNNTSGNPFWNFIGDFIAKPTPDQIRCQSPKPILLDVG-M 524

Query: 301 KTPYDWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHV 360
             P  W P ++PIQI+ IGQ+V++ VPGEFTTM+GRRLR++V+ ++      E   N  V
Sbjct: 525 VEPIPWVPDVMPIQIVTIGQIVLVAVPGEFTTMSGRRLRNSVREII-----GESIENPIV 584

Query: 361 VIAGLTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGP 420
           +IAGL+NTYS Y+ TFEEY++QRYEGAST++GPHTL +Y+QEF KLAQS++DGT V  GP
Sbjct: 585 LIAGLSNTYSGYIATFEEYQVQRYEGASTVFGPHTLGSYMQEFGKLAQSIVDGTTVPAGP 644

Query: 421 QPPDLLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLM 480
            P +L    +  LPPVI+D  P    FG+V  DV  N ++     V   F+ G PRND M
Sbjct: 645 TPRNLTGHTLFFLPPVIVDAAPDFDDFGEVSIDV--NLNYSVNETVSCVFYGGNPRNDFM 704

Query: 481 TEGTFALVEILQ-KNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYR 540
            E +F  V++L   + W    DD D+  +FKW         S  TIEW I      G YR
Sbjct: 705 IESSFLSVDLLTGTDQWTTVLDDGDWDTKFKWKMHD--LGFSLITIEWVIAPDTTPGTYR 717

Query: 541 IRHFGAAKS--LLGSIRHFTGSSSAFVV 562
           I H G AK      ++  + G S  FVV
Sbjct: 765 ITHSGFAKKNPFSSNLTPYQGISRNFVV 717

[Close]

BLAST of Cucsat.G8367 vs. NCBI nr
Match: XP_004153679.2 (neutral ceramidase 2 [Cucumis sativus] >KGN65777.1 hypothetical protein Csa_023365 [Cucumis sativus])

HSP 1 Score: 1146 bits (2965), Expect = 0.0
Identity = 562/562 (100.00%), Postives = 562/562 (100.00%), Query Frame = 0


Query: 1   MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHH 60
           MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHH
Sbjct: 218 MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHH 277

Query: 61  GESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADR 120
           GESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADR
Sbjct: 278 GESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADR 337

Query: 121 PQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 180
           PQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR
Sbjct: 338 PQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 397

Query: 181 IIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGF 240
           IIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGF
Sbjct: 398 IIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGF 457

Query: 241 AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPY 300
           AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPY
Sbjct: 458 AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPY 517

Query: 301 DWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAG 360
           DWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAG
Sbjct: 518 DWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAG 577

Query: 361 LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPD 420
           LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPD
Sbjct: 578 LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPD 637

Query: 421 LLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGT 480
           LLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGT
Sbjct: 638 LLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGT 697

Query: 481 FALVEILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFG 540
           FALVEILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFG
Sbjct: 698 FALVEILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFG 757

Query: 541 AAKSLLGSIRHFTGSSSAFVVA 562
           AAKSLLGSIRHFTGSSSAFVVA
Sbjct: 758 AAKSLLGSIRHFTGSSSAFVVA 779

[Close]

BLAST of Cucsat.G8367 vs. NCBI nr
Match: XP_008457413.1 (PREDICTED: neutral ceramidase-like isoform X2 [Cucumis melo])

HSP 1 Score: 1091 bits (2822), Expect = 0.0
Identity = 532/561 (94.83%), Postives = 539/561 (96.08%), Query Frame = 0


Query: 1   MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHH 60
           MTLLKFIDDEWGPVGTFNWFATHGTSMSRTN+LISGDNKGAAARFMEDWF+QKGTGTLH 
Sbjct: 122 MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFEQKGTGTLHP 181

Query: 61  GESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADR 120
           GESEADSIPRRVSNI+PEVYKDKQELLE AASFQSQPGRPATR LS+SSRVRNVLRQADR
Sbjct: 182 GESEADSIPRRVSNIIPEVYKDKQELLERAASFQSQPGRPATRALSVSSRVRNVLRQADR 241

Query: 121 PQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 180
           PQFVSAFCQSNCGDVSPNTLGAFCLDT LPCDFNHSTCGGKNELCYGRGPGYPDEFESTR
Sbjct: 242 PQFVSAFCQSNCGDVSPNTLGAFCLDTELPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 301

Query: 181 IIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGF 240
           IIGE+QFRKAVDLF KASE LTGKVDFRHSYVDFSHLEVSL KQGG  EVVKTCPAAMGF
Sbjct: 302 IIGERQFRKAVDLFGKASEHLTGKVDFRHSYVDFSHLEVSLTKQGGAAEVVKTCPAAMGF 361

Query: 241 AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPY 300
           AFAAGTTDGPGAFDFKQGDDKGN FWKLVRN+LKAPGNEQISCQSPKPILLDTGEMK PY
Sbjct: 362 AFAAGTTDGPGAFDFKQGDDKGNPFWKLVRNLLKAPGNEQISCQSPKPILLDTGEMKIPY 421

Query: 301 DWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAG 360
           DWAP+ILPIQILRIGQLVIL VPGEFTTMAGRRLRDAVKTVLTTGA KEFNSNVHVVIAG
Sbjct: 422 DWAPTILPIQILRIGQLVILSVPGEFTTMAGRRLRDAVKTVLTTGANKEFNSNVHVVIAG 481

Query: 361 LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPD 420
           LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDG PV PGPQPPD
Sbjct: 482 LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGRPVVPGPQPPD 541

Query: 421 LLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGT 480
           LL RQISLLPPVILDMTP GVSFGDVK DVP NSSFKRGNLVKVTFWTGCPRNDLMTEGT
Sbjct: 542 LLDRQISLLPPVILDMTPPGVSFGDVKSDVPRNSSFKRGNLVKVTFWTGCPRNDLMTEGT 601

Query: 481 FALVEILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFG 540
           FALVEILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQT VSGVYRIRHFG
Sbjct: 602 FALVEILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTVVSGVYRIRHFG 661

Query: 541 AAKSLLGSIRHFTGSSSAFVV 561
           AAKS  GSIRHFTGSSSAFVV
Sbjct: 662 AAKSFFGSIRHFTGSSSAFVV 682

[Close]

BLAST of Cucsat.G8367 vs. NCBI nr
Match: XP_008457412.1 (PREDICTED: neutral ceramidase-like isoform X1 [Cucumis melo])

HSP 1 Score: 1091 bits (2822), Expect = 0.0
Identity = 532/561 (94.83%), Postives = 539/561 (96.08%), Query Frame = 0


Query: 1   MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHH 60
           MTLLKFIDDEWGPVGTFNWFATHGTSMSRTN+LISGDNKGAAARFMEDWF+QKGTGTLH 
Sbjct: 218 MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFEQKGTGTLHP 277

Query: 61  GESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADR 120
           GESEADSIPRRVSNI+PEVYKDKQELLE AASFQSQPGRPATR LS+SSRVRNVLRQADR
Sbjct: 278 GESEADSIPRRVSNIIPEVYKDKQELLERAASFQSQPGRPATRALSVSSRVRNVLRQADR 337

Query: 121 PQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 180
           PQFVSAFCQSNCGDVSPNTLGAFCLDT LPCDFNHSTCGGKNELCYGRGPGYPDEFESTR
Sbjct: 338 PQFVSAFCQSNCGDVSPNTLGAFCLDTELPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 397

Query: 181 IIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGF 240
           IIGE+QFRKAVDLF KASE LTGKVDFRHSYVDFSHLEVSL KQGG  EVVKTCPAAMGF
Sbjct: 398 IIGERQFRKAVDLFGKASEHLTGKVDFRHSYVDFSHLEVSLTKQGGAAEVVKTCPAAMGF 457

Query: 241 AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPY 300
           AFAAGTTDGPGAFDFKQGDDKGN FWKLVRN+LKAPGNEQISCQSPKPILLDTGEMK PY
Sbjct: 458 AFAAGTTDGPGAFDFKQGDDKGNPFWKLVRNLLKAPGNEQISCQSPKPILLDTGEMKIPY 517

Query: 301 DWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAG 360
           DWAP+ILPIQILRIGQLVIL VPGEFTTMAGRRLRDAVKTVLTTGA KEFNSNVHVVIAG
Sbjct: 518 DWAPTILPIQILRIGQLVILSVPGEFTTMAGRRLRDAVKTVLTTGANKEFNSNVHVVIAG 577

Query: 361 LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPD 420
           LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDG PV PGPQPPD
Sbjct: 578 LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGRPVVPGPQPPD 637

Query: 421 LLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGT 480
           LL RQISLLPPVILDMTP GVSFGDVK DVP NSSFKRGNLVKVTFWTGCPRNDLMTEGT
Sbjct: 638 LLDRQISLLPPVILDMTPPGVSFGDVKSDVPRNSSFKRGNLVKVTFWTGCPRNDLMTEGT 697

Query: 481 FALVEILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFG 540
           FALVEILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQT VSGVYRIRHFG
Sbjct: 698 FALVEILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTVVSGVYRIRHFG 757

Query: 541 AAKSLLGSIRHFTGSSSAFVV 561
           AAKS  GSIRHFTGSSSAFVV
Sbjct: 758 AAKSFFGSIRHFTGSSSAFVV 778

[Close]

BLAST of Cucsat.G8367 vs. NCBI nr
Match: XP_038892835.1 (neutral ceramidase 2-like [Benincasa hispida])

HSP 1 Score: 1066 bits (2756), Expect = 0.0
Identity = 516/563 (91.65%), Postives = 544/563 (96.63%), Query Frame = 0


Query: 1   MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHH 60
           MTLLKF+DDEWGPVG+FNWFATHGTSMSRTN+LISGDNKGAAARFMEDWF+QKGTGT+H 
Sbjct: 218 MTLLKFVDDEWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFEQKGTGTMHP 277

Query: 61  GESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADR 120
           GESE DSIPRRVSNI+PEV+K+KQELLE AASFQSQPGRPATR+LS+S RVRNVLRQA+R
Sbjct: 278 GESEVDSIPRRVSNIIPEVFKNKQELLERAASFQSQPGRPATRILSVSRRVRNVLRQANR 337

Query: 121 PQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 180
           PQFVSAFCQSNCGDVSPNTLGAFC+DTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR
Sbjct: 338 PQFVSAFCQSNCGDVSPNTLGAFCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 397

Query: 181 IIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGF 240
           IIGE+QFRKAVDLFSKASEQLTGKVDFRHSYVDFS LEVSL+KQGG TEV++TCPAAMGF
Sbjct: 398 IIGERQFRKAVDLFSKASEQLTGKVDFRHSYVDFSQLEVSLSKQGGATEVIRTCPAAMGF 457

Query: 241 AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPY 300
           AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRN+LKAPGNEQI+CQSPKPILLDTGEMK PY
Sbjct: 458 AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKIPY 517

Query: 301 DWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAG 360
           DWAPSILPIQILRIGQLVIL VPGEFTTMAGRRLRDAVKTVLTTGAK+EFN NVHVVIAG
Sbjct: 518 DWAPSILPIQILRIGQLVILGVPGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAG 577

Query: 361 LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPD 420
           LTNTYSQYVTTFEEY+MQRYEGASTLYGPHTL+AYIQEFKKLAQSLIDG PV PGPQPPD
Sbjct: 578 LTNTYSQYVTTFEEYEMQRYEGASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPD 637

Query: 421 LLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGT 480
           LLARQISLL PVI+D TP+GVSFGDVK DVP NS+FKRGNLV V+FW+GCPRNDLMTEGT
Sbjct: 638 LLARQISLLTPVIMDATPIGVSFGDVKSDVPLNSTFKRGNLVTVSFWSGCPRNDLMTEGT 697

Query: 481 FALVEILQK-NTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHF 540
           FALVEILQK +TWVPAYDDDDFCLRFKWSRP+ LS QSYATIEWRIPQTAVSGVYRIRHF
Sbjct: 698 FALVEILQKKDTWVPAYDDDDFCLRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHF 757

Query: 541 GAAKSLLGSIRHFTGSSSAFVVA 562
           GAAKSLLGSIRHFTGSSSAFVVA
Sbjct: 758 GAAKSLLGSIRHFTGSSSAFVVA 780

[Close]

BLAST of Cucsat.G8367 vs. NCBI nr
Match: KAG6583392.1 (Neutral ceramidase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1036 bits (2680), Expect = 0.0
Identity = 503/563 (89.34%), Postives = 533/563 (94.67%), Query Frame = 0


Query: 1   MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHH 60
           MTLLKFIDD+WGPVG+FNWFATHGTSMSRTN+LISGDNKGAAARFMEDWF QKG GTL  
Sbjct: 218 MTLLKFIDDQWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFVQKGIGTLQT 277

Query: 61  GESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADR 120
           G+SEADSIPRRVSNI+PE YKD+QELL  AASFQS+PGRPATR+LS+S RVRNVLRQADR
Sbjct: 278 GKSEADSIPRRVSNIIPEAYKDEQELLARAASFQSRPGRPATRILSVSRRVRNVLRQADR 337

Query: 121 PQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 180
           PQFVSAFCQSNCGDVSPNTLGAFC+DTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR
Sbjct: 338 PQFVSAFCQSNCGDVSPNTLGAFCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 397

Query: 181 IIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGF 240
           IIGE+QFRKAVDLFSKASEQL G VD+RHSYVDFS LEVS+ K GG TEVVKTCPAAMGF
Sbjct: 398 IIGERQFRKAVDLFSKASEQLKGTVDYRHSYVDFSQLEVSVTKAGGATEVVKTCPAAMGF 457

Query: 241 AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPY 300
           AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRN+LK PG++QI+CQSPKPILLDTGEMK PY
Sbjct: 458 AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNLLKTPGSKQIACQSPKPILLDTGEMKIPY 517

Query: 301 DWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAG 360
           DWAPSILPIQILRIGQLVIL VPGEFTTMAGRRLRDAVKTVLTTGAK+EFN NVHVVIAG
Sbjct: 518 DWAPSILPIQILRIGQLVILGVPGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAG 577

Query: 361 LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPD 420
           LTNTYSQYVTTFEEY+MQRYEGASTLYGPHTL+AYIQEF+KLAQSLIDG PV PGPQPPD
Sbjct: 578 LTNTYSQYVTTFEEYEMQRYEGASTLYGPHTLDAYIQEFRKLAQSLIDGRPVEPGPQPPD 637

Query: 421 LLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGT 480
           LLA+QISLL PV++D TP GVSFGDVK DVP NS+FKRG+LV VTFW+GCPRNDLMTEGT
Sbjct: 638 LLAKQISLLTPVVMDATPFGVSFGDVKSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGT 697

Query: 481 FALVEILQK-NTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHF 540
           FALVEILQK +TWVP+YDDDDFCLRFKWSRP+ LS QS ATIEWRIPQTAVSGVYRIRHF
Sbjct: 698 FALVEILQKKDTWVPSYDDDDFCLRFKWSRPSRLSPQSLATIEWRIPQTAVSGVYRIRHF 757

Query: 541 GAAKSLLGSIRHFTGSSSAFVVA 562
           GAAKSLLGSIRHFTGSSSAFVVA
Sbjct: 758 GAAKSLLGSIRHFTGSSSAFVVA 780

[Close]

BLAST of Cucsat.G8367 vs. ExPASy TrEMBL
Match: A0A0A0LY63 (Neutral ceramidase OS=Cucumis sativus OX=3659 GN=Csa_1G527930 PE=3 SV=1)

HSP 1 Score: 1146 bits (2965), Expect = 0.0
Identity = 562/562 (100.00%), Postives = 562/562 (100.00%), Query Frame = 0


Query: 1   MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHH 60
           MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHH
Sbjct: 218 MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHH 277

Query: 61  GESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADR 120
           GESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADR
Sbjct: 278 GESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADR 337

Query: 121 PQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 180
           PQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR
Sbjct: 338 PQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 397

Query: 181 IIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGF 240
           IIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGF
Sbjct: 398 IIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGF 457

Query: 241 AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPY 300
           AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPY
Sbjct: 458 AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPY 517

Query: 301 DWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAG 360
           DWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAG
Sbjct: 518 DWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAG 577

Query: 361 LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPD 420
           LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPD
Sbjct: 578 LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPD 637

Query: 421 LLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGT 480
           LLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGT
Sbjct: 638 LLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGT 697

Query: 481 FALVEILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFG 540
           FALVEILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFG
Sbjct: 698 FALVEILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFG 757

Query: 541 AAKSLLGSIRHFTGSSSAFVVA 562
           AAKSLLGSIRHFTGSSSAFVVA
Sbjct: 758 AAKSLLGSIRHFTGSSSAFVVA 779

[Close]

BLAST of Cucsat.G8367 vs. ExPASy TrEMBL
Match: A0A1S3C5F7 (Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103497106 PE=3 SV=1)

HSP 1 Score: 1091 bits (2822), Expect = 0.0
Identity = 532/561 (94.83%), Postives = 539/561 (96.08%), Query Frame = 0


Query: 1   MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHH 60
           MTLLKFIDDEWGPVGTFNWFATHGTSMSRTN+LISGDNKGAAARFMEDWF+QKGTGTLH 
Sbjct: 218 MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFEQKGTGTLHP 277

Query: 61  GESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADR 120
           GESEADSIPRRVSNI+PEVYKDKQELLE AASFQSQPGRPATR LS+SSRVRNVLRQADR
Sbjct: 278 GESEADSIPRRVSNIIPEVYKDKQELLERAASFQSQPGRPATRALSVSSRVRNVLRQADR 337

Query: 121 PQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 180
           PQFVSAFCQSNCGDVSPNTLGAFCLDT LPCDFNHSTCGGKNELCYGRGPGYPDEFESTR
Sbjct: 338 PQFVSAFCQSNCGDVSPNTLGAFCLDTELPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 397

Query: 181 IIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGF 240
           IIGE+QFRKAVDLF KASE LTGKVDFRHSYVDFSHLEVSL KQGG  EVVKTCPAAMGF
Sbjct: 398 IIGERQFRKAVDLFGKASEHLTGKVDFRHSYVDFSHLEVSLTKQGGAAEVVKTCPAAMGF 457

Query: 241 AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPY 300
           AFAAGTTDGPGAFDFKQGDDKGN FWKLVRN+LKAPGNEQISCQSPKPILLDTGEMK PY
Sbjct: 458 AFAAGTTDGPGAFDFKQGDDKGNPFWKLVRNLLKAPGNEQISCQSPKPILLDTGEMKIPY 517

Query: 301 DWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAG 360
           DWAP+ILPIQILRIGQLVIL VPGEFTTMAGRRLRDAVKTVLTTGA KEFNSNVHVVIAG
Sbjct: 518 DWAPTILPIQILRIGQLVILSVPGEFTTMAGRRLRDAVKTVLTTGANKEFNSNVHVVIAG 577

Query: 361 LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPD 420
           LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDG PV PGPQPPD
Sbjct: 578 LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGRPVVPGPQPPD 637

Query: 421 LLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGT 480
           LL RQISLLPPVILDMTP GVSFGDVK DVP NSSFKRGNLVKVTFWTGCPRNDLMTEGT
Sbjct: 638 LLDRQISLLPPVILDMTPPGVSFGDVKSDVPRNSSFKRGNLVKVTFWTGCPRNDLMTEGT 697

Query: 481 FALVEILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFG 540
           FALVEILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQT VSGVYRIRHFG
Sbjct: 698 FALVEILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTVVSGVYRIRHFG 757

Query: 541 AAKSLLGSIRHFTGSSSAFVV 561
           AAKS  GSIRHFTGSSSAFVV
Sbjct: 758 AAKSFFGSIRHFTGSSSAFVV 778

[Close]

BLAST of Cucsat.G8367 vs. ExPASy TrEMBL
Match: A0A1S3C650 (Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103497106 PE=3 SV=1)

HSP 1 Score: 1091 bits (2822), Expect = 0.0
Identity = 532/561 (94.83%), Postives = 539/561 (96.08%), Query Frame = 0


Query: 1   MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHH 60
           MTLLKFIDDEWGPVGTFNWFATHGTSMSRTN+LISGDNKGAAARFMEDWF+QKGTGTLH 
Sbjct: 122 MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFEQKGTGTLHP 181

Query: 61  GESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADR 120
           GESEADSIPRRVSNI+PEVYKDKQELLE AASFQSQPGRPATR LS+SSRVRNVLRQADR
Sbjct: 182 GESEADSIPRRVSNIIPEVYKDKQELLERAASFQSQPGRPATRALSVSSRVRNVLRQADR 241

Query: 121 PQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 180
           PQFVSAFCQSNCGDVSPNTLGAFCLDT LPCDFNHSTCGGKNELCYGRGPGYPDEFESTR
Sbjct: 242 PQFVSAFCQSNCGDVSPNTLGAFCLDTELPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 301

Query: 181 IIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGF 240
           IIGE+QFRKAVDLF KASE LTGKVDFRHSYVDFSHLEVSL KQGG  EVVKTCPAAMGF
Sbjct: 302 IIGERQFRKAVDLFGKASEHLTGKVDFRHSYVDFSHLEVSLTKQGGAAEVVKTCPAAMGF 361

Query: 241 AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPY 300
           AFAAGTTDGPGAFDFKQGDDKGN FWKLVRN+LKAPGNEQISCQSPKPILLDTGEMK PY
Sbjct: 362 AFAAGTTDGPGAFDFKQGDDKGNPFWKLVRNLLKAPGNEQISCQSPKPILLDTGEMKIPY 421

Query: 301 DWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAG 360
           DWAP+ILPIQILRIGQLVIL VPGEFTTMAGRRLRDAVKTVLTTGA KEFNSNVHVVIAG
Sbjct: 422 DWAPTILPIQILRIGQLVILSVPGEFTTMAGRRLRDAVKTVLTTGANKEFNSNVHVVIAG 481

Query: 361 LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPD 420
           LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDG PV PGPQPPD
Sbjct: 482 LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGRPVVPGPQPPD 541

Query: 421 LLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGT 480
           LL RQISLLPPVILDMTP GVSFGDVK DVP NSSFKRGNLVKVTFWTGCPRNDLMTEGT
Sbjct: 542 LLDRQISLLPPVILDMTPPGVSFGDVKSDVPRNSSFKRGNLVKVTFWTGCPRNDLMTEGT 601

Query: 481 FALVEILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFG 540
           FALVEILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQT VSGVYRIRHFG
Sbjct: 602 FALVEILQKNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTVVSGVYRIRHFG 661

Query: 541 AAKSLLGSIRHFTGSSSAFVV 561
           AAKS  GSIRHFTGSSSAFVV
Sbjct: 662 AAKSFFGSIRHFTGSSSAFVV 682

[Close]

BLAST of Cucsat.G8367 vs. ExPASy TrEMBL
Match: A0A6J1DAR9 (Neutral ceramidase OS=Momordica charantia OX=3673 GN=LOC111018960 PE=3 SV=1)

HSP 1 Score: 1034 bits (2673), Expect = 0.0
Identity = 499/563 (88.63%), Postives = 531/563 (94.32%), Query Frame = 0


Query: 1   MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHH 60
           MTLLKF+DDEWGPVG+FNWFATHGTSMSRTN+LISGDNKGAAARFMEDWF QKG G+LH 
Sbjct: 218 MTLLKFVDDEWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFDQKGIGSLHP 277

Query: 61  GESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADR 120
           G+S ADSIPRRVSNI+PE YK+KQELLE AASFQS+PGRPATR+LS++ RVRNVLRQADR
Sbjct: 278 GQSVADSIPRRVSNIIPEAYKNKQELLERAASFQSRPGRPATRILSVARRVRNVLRQADR 337

Query: 121 PQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 180
           PQFVSAFCQSNCGDVSPN LGAFC+DTGLPCDFNHSTCGGKNELCYGRGPGYPDEFES R
Sbjct: 338 PQFVSAFCQSNCGDVSPNVLGAFCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESAR 397

Query: 181 IIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGF 240
           IIGE+QFRKAVDLF+KASE+LTGKVD+RHSYVDFS LEVSL KQGG TEVVKTCPAAMGF
Sbjct: 398 IIGERQFRKAVDLFNKASEKLTGKVDYRHSYVDFSQLEVSLLKQGGATEVVKTCPAAMGF 457

Query: 241 AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPY 300
           AFAAGTTDGPGAFDFKQGDDKGN FWKLVRN+L+APG +Q++CQSPKPILLDTGEMK PY
Sbjct: 458 AFAAGTTDGPGAFDFKQGDDKGNPFWKLVRNLLRAPGEQQVTCQSPKPILLDTGEMKAPY 517

Query: 301 DWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAG 360
           DWAPSILPIQILRIGQLVIL VPGEFTTMAGRRLR+AVKTVLTTGAK+EFN NVH+VIAG
Sbjct: 518 DWAPSILPIQILRIGQLVILGVPGEFTTMAGRRLRNAVKTVLTTGAKREFNGNVHIVIAG 577

Query: 361 LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPD 420
           LTNTYSQYVTTFEEY+MQRYEGASTLYGPHTL+AYIQEFKKLAQSLIDG PV PGPQPPD
Sbjct: 578 LTNTYSQYVTTFEEYEMQRYEGASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPD 637

Query: 421 LLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGT 480
           LL+RQISLLPPVI+D TPLGVSFGDVK DVP NS+FKRGNLV VTFW+GCPRNDLMTEGT
Sbjct: 638 LLSRQISLLPPVIMDATPLGVSFGDVKSDVPLNSTFKRGNLVTVTFWSGCPRNDLMTEGT 697

Query: 481 FALVEILQK-NTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHF 540
           FALVEILQK +TWVP YDDDDFCL FKW RP   S QSYATIEWRIPQ+AVSGVYRIRHF
Sbjct: 698 FALVEILQKKDTWVPVYDDDDFCLFFKWLRPFRYSTQSYATIEWRIPQSAVSGVYRIRHF 757

Query: 541 GAAKSLLGSIRHFTGSSSAFVVA 562
           GAAKSL GSIRHFTGSSSAFVVA
Sbjct: 758 GAAKSLFGSIRHFTGSSSAFVVA 780

[Close]

BLAST of Cucsat.G8367 vs. ExPASy TrEMBL
Match: A0A6J1HIT9 (Neutral ceramidase OS=Cucurbita moschata OX=3662 GN=LOC111464833 PE=3 SV=1)

HSP 1 Score: 1033 bits (2672), Expect = 0.0
Identity = 501/563 (88.99%), Postives = 532/563 (94.49%), Query Frame = 0


Query: 1   MTLLKFIDDEWGPVGTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHH 60
           MTLLKFIDD+WGPVG+FNWFATHGTSMSRTN+LISGDNKGAAARFMEDWF QKG GTL  
Sbjct: 218 MTLLKFIDDQWGPVGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFVQKGIGTLQT 277

Query: 61  GESEADSIPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADR 120
           G+SEADSIPRRVSNI+PE Y D+QELL  AASFQS+PGRPATR+LS+S RVRNVLRQADR
Sbjct: 278 GKSEADSIPRRVSNIIPEAYNDEQELLARAASFQSRPGRPATRILSVSRRVRNVLRQADR 337

Query: 121 PQFVSAFCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 180
           PQFVSAFCQSNCGDVSPNTLGAFC+DTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR
Sbjct: 338 PQFVSAFCQSNCGDVSPNTLGAFCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTR 397

Query: 181 IIGEKQFRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGF 240
           IIGE+QFRKAVDLFSKASEQL G VD+RHSYVDFS LEVS+ K GG TEVVKTCPAAMGF
Sbjct: 398 IIGERQFRKAVDLFSKASEQLKGTVDYRHSYVDFSQLEVSVTKAGGATEVVKTCPAAMGF 457

Query: 241 AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPY 300
           AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRN+LK PG++QI+CQSPKPILLDTGEMK PY
Sbjct: 458 AFAAGTTDGPGAFDFKQGDDKGNAFWKLVRNLLKTPGSKQIACQSPKPILLDTGEMKIPY 517

Query: 301 DWAPSILPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAG 360
           DWAPSILPIQILRIGQLVIL VPGEFTTMAGRRLRDAVKTVLTTGAK+EFN NVHVVIAG
Sbjct: 518 DWAPSILPIQILRIGQLVILGVPGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAG 577

Query: 361 LTNTYSQYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPD 420
           LTNTYSQYVTTFEEY+MQRYEGASTLYGPHTL+AYIQEF+KLAQSLIDG PV PGPQPPD
Sbjct: 578 LTNTYSQYVTTFEEYEMQRYEGASTLYGPHTLDAYIQEFRKLAQSLIDGRPVEPGPQPPD 637

Query: 421 LLARQISLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGT 480
           LLA+QISLL PV++D TP GVSFGDV+ DVP NS+FKRG+LV VTFW+GCPRNDLMTEGT
Sbjct: 638 LLAKQISLLTPVVMDATPFGVSFGDVRSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGT 697

Query: 481 FALVEILQK-NTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHF 540
           FALVEILQK +TWVP+YDDDDFCLRFKWSRP+ LS QS ATIEWRIPQTAVSGVYRIRHF
Sbjct: 698 FALVEILQKKDTWVPSYDDDDFCLRFKWSRPSRLSPQSLATIEWRIPQTAVSGVYRIRHF 757

Query: 541 GAAKSLLGSIRHFTGSSSAFVVA 562
           GAAKSLLGSIRHFTGSSSAFVVA
Sbjct: 758 GAAKSLLGSIRHFTGSSSAFVVA 780

The following BLAST results are available for this feature:

BLAST of Cucsat.G8367 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4HQM3	4.5e-244	71.99	Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1	[more]
Q304B9	1.4e-240	70.11	Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1	[more]
Q0JL46	1.4e-232	67.54	Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1...	[more]
F4KHQ8	1.8e-216	66.73	Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1	[more]
Q55G11	4.7e-116	43.84	Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1	[more]

BLAST of Cucsat.G8367 vs. NCBI nr


Match Name	Identity	Description
XP_004153679.2	100.00	neutral ceramidase 2 [Cucumis sativus] >KGN65777.1 hypothetical protein Csa_0233...	[more]
XP_008457413.1	94.83	PREDICTED: neutral ceramidase-like isoform X2 [Cucumis melo]	[more]
XP_008457412.1	94.83	PREDICTED: neutral ceramidase-like isoform X1 [Cucumis melo]	[more]
XP_038892835.1	91.65	neutral ceramidase 2-like [Benincasa hispida]	[more]
KAG6583392.1	89.34	Neutral ceramidase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Cucsat.G8367 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A0A0LY63	100.00	Neutral ceramidase OS=Cucumis sativus OX=3659 GN=Csa_1G527930 PE=3 SV=1	[more]
A0A1S3C5F7	94.83	Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103497106 PE=3 SV=1	[more]
A0A1S3C650	94.83	Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103497106 PE=3 SV=1	[more]
A0A6J1DAR9	88.63	Neutral ceramidase OS=Momordica charantia OX=3673 GN=LOC111018960 PE=3 SV=1	[more]
A0A6J1HIT9	88.99	Neutral ceramidase OS=Cucurbita moschata OX=3662 GN=LOC111464833 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR038445	Neutral ceramidase, C-terminal domain superfamily	GENE3D	2.60.40.2300		coord: 430..562 e-value: 9.7E-45 score: 153.9
IPR031331	Neutral/alkaline non-lysosomal ceramidase, C-terminal	PFAM	PF17048	Ceramidse_alk_C	coord: 399..561 e-value: 6.0E-52 score: 175.8
IPR031329	Neutral/alkaline non-lysosomal ceramidase, N-terminal	PFAM	PF04734	Ceramidase_alk	coord: 1..397 e-value: 2.0E-145 score: 485.3
IPR006823	Neutral/alkaline nonlysosomal ceramidase	PANTHER	PTHR12670	CERAMIDASE	coord: 1..562
None	No IPR available	PANTHER	PTHR12670:SF17	NEUTRAL CERAMIDASE 2	coord: 1..562

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G8367.T10	Cucsat.G8367.T10	mRNA
Cucsat.G8367.T9	Cucsat.G8367.T9	mRNA
Cucsat.G8367.T8	Cucsat.G8367.T8	mRNA
Cucsat.G8367.T5	Cucsat.G8367.T5	mRNA
Cucsat.G8367.T4	Cucsat.G8367.T4	mRNA
Cucsat.G8367.T1	Cucsat.G8367.T1	mRNA
Cucsat.G8367.T7	Cucsat.G8367.T7	mRNA
Cucsat.G8367.T6	Cucsat.G8367.T6	mRNA
Cucsat.G8367.T12	Cucsat.G8367.T12	mRNA
Cucsat.G8367.T11	Cucsat.G8367.T11	mRNA
Cucsat.G8367.T3	Cucsat.G8367.T3	mRNA
Cucsat.G8367.T2	Cucsat.G8367.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046514	ceramide catabolic process
biological_process	GO:0042759	long-chain fatty acid biosynthetic process
biological_process	GO:0046512	sphingosine biosynthetic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0102121	ceramidase activity
molecular_function	GO:0017040	N-acylsphingosine amidohydrolase activity