Cucsat.G8367.T8 (mRNA) Cucumber (B10) v3

BLAST of Cucsat.G8367.T8 vs. ExPASy Swiss-Prot
Match: F4HQM3 (Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1)

HSP 1 Score: 1026.2 bits (2652), Expect = 1.7e-298
Identity = 494/670 (73.73%), Postives = 561/670 (83.73%), Query Frame = 0

Query: 8   VRKRYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQA 67
           ++ RYGDLYTE+NV ISGIH+HAGPGGYLQYVVYIVTSLGFVRQSF+ LVDGIE SIIQA
Sbjct: 109 LKARYGDLYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDALVDGIENSIIQA 168

Query: 68  HENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPV 127
           HENL PGSI +N GEL+DAGVNRSPSAYLNNP+ ERSK+KY+VDKEMTLLKF+DD+WGPV
Sbjct: 169 HENLRPGSIFLNNGELLDAGVNRSPSAYLNNPSKERSKHKYNVDKEMTLLKFVDDQWGPV 228

Query: 128 GTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSN 187
           G+FNWFATHGTSMSRTN+LISGDNKGAA+RFMEDW++Q      +  E  +D IPRRVS+
Sbjct: 229 GSFNWFATHGTSMSRTNSLISGDNKGAASRFMEDWYEQNTAERSYSEEFISDEIPRRVSS 288

Query: 188 IVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGD 247
           ++        ELLELA+ F+SQPG+P TR+ S + RVR+ LR+AD+P FVSAFCQ+NCGD
Sbjct: 289 LIENHQDSHHELLELASYFESQPGKPVTRISSSARRVRSALRKADKPGFVSAFCQTNCGD 348

Query: 248 VSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLF 307
           VSPN LGAFCLDTGLPCDFNHSTCGGKNE+CYGRGPGYPDEFESTRIIGE+QF+ A++LF
Sbjct: 349 VSPNVLGAFCLDTGLPCDFNHSTCGGKNEMCYGRGPGYPDEFESTRIIGERQFKMALELF 408

Query: 308 SKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFD 367
           +KASEQL GKVD+RH YVDFS L V+L K+ G +EVVKTCPAAMGFAFAAGTTDGPGAFD
Sbjct: 409 NKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPAAMGFAFAAGTTDGPGAFD 468

Query: 368 FKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRI 427
           F QGDDKGN FW+LVRNVLK P  +QI C  PKPILLDTGEM  PYDWAPSIL +Q+LRI
Sbjct: 469 FTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEMTKPYDWAPSILSLQVLRI 528

Query: 428 GQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEE 487
           GQL IL VPGEFTTMAGRRLR AVKT L     K+ +  +HVVIAGL N YSQYVTTFEE
Sbjct: 529 GQLFILSVPGEFTTMAGRRLRYAVKTQLKNSGNKDLSGEIHVVIAGLANGYSQYVTTFEE 588

Query: 488 YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVIL 547
           Y++QRYEGASTLYGPHTL  YIQEFKKL++SL+   PV PGPQPPDLL +Q+S L PV++
Sbjct: 589 YQVQRYEGASTLYGPHTLSGYIQEFKKLSKSLVLDMPVQPGPQPPDLLDKQLSFLTPVMM 648

Query: 548 DMTPLGVSFGDVKFDVPSNSSFKRGN-LVKVTFWTGCPRNDLMTEGTFALVEILQK--NT 607
           D TP G SFGDV  DVP N S KRGN  V V F + CPRNDL+TEGTF LVE L++   T
Sbjct: 649 DTTPSGDSFGDVISDVPKNLSLKRGNGQVTVVFRSACPRNDLLTEGTFTLVERLEQKDKT 708

Query: 608 WVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRH 667
           W P YDDDD CLRFKWSR   LS++S AT+EWRIP++A  GVYRI HFGAAK L GS+ H
Sbjct: 709 WTPVYDDDDLCLRFKWSRHKKLSSRSQATVEWRIPESASPGVYRITHFGAAKKLFGSVHH 768

Query: 668 FTGSSSAFVV 675
           FTGSSSAFVV
Sbjct: 769 FTGSSSAFVV 778

BLAST of Cucsat.G8367.T8 vs. ExPASy Swiss-Prot
Match: Q304B9 (Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1)

HSP 1 Score: 1014.6 bits (2622), Expect = 5.2e-295
Identity = 480/668 (71.86%), Postives = 573/668 (85.78%), Query Frame = 0

Query: 8   VRKRYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQA 67
           ++ RYG+LYTEKNVAISGIH+HAGPGGYLQYV YIVTSLGFVRQSF+V+V+GIE+SI+QA
Sbjct: 98  LKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFDVVVNGIEQSIVQA 157

Query: 68  HENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPV 127
           HE+L PGS  +NKG+L+DAGVNRSPS+YLNNPA+ERSKYKYDVDKEMTL+KF+D + GP 
Sbjct: 158 HESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEMTLVKFVDSQLGPT 217

Query: 128 GTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSN 187
           G+FNWFATHGTSMSRTN+LISGDNKGAAARFMEDWF+     ++      + +IPRRVS 
Sbjct: 218 GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSV-----SSRNIPRRVST 277

Query: 188 IVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGD 247
           IV +  +++  LL++AA+++S  G    + L + +RVRN      + +FVSAFCQSNCGD
Sbjct: 278 IVSDFSRNRDRLLDIAATYKSSRGHSVDKSLDVKTRVRN----GSKRKFVSAFCQSNCGD 337

Query: 248 VSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLF 307
           VSPNTLG FC+DTGLPCDFNHSTC G+NELCYGRGPGYPDEFESTRIIGEKQF+ AV+LF
Sbjct: 338 VSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELF 397

Query: 308 SKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFD 367
           +KA+E+L GK+ ++H+Y+DFS+L+V++ K GG +E VKTCPAAMGF FAAGTTDGPGAFD
Sbjct: 398 NKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFD 457

Query: 368 FKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRI 427
           FKQGDD+GN FW+LVRNVL+ PG EQ+ CQ PKPILLDTGEMK PYDWAPSILPIQILRI
Sbjct: 458 FKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRI 517

Query: 428 GQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEE 487
           GQLVIL VPGEFTTMAGRRLRDA+K+ L +   KEF++N+HVVIAGLTNTYSQY+ TFEE
Sbjct: 518 GQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEE 577

Query: 488 YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVIL 547
           Y++QRYEGASTLYG HTL AYIQEFKKLA +L++G  +  GPQPPDLL +QISLL PV++
Sbjct: 578 YEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTLPRGPQPPDLLDKQISLLSPVVV 637

Query: 548 DMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQK-NTWV 607
           D TPLGV FGDVK DVP  S+F+RG  V  TFW+GCPRNDLMTEG+FA+VE L++   W 
Sbjct: 638 DSTPLGVKFGDVKADVPPKSTFRRGQQVNATFWSGCPRNDLMTEGSFAVVETLREGGKWA 697

Query: 608 PAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFT 667
           P YDDDDF L+FKWSRPA LS++S ATIEWR+P++AV+GVYRIRH+GA+KSL GSI  F+
Sbjct: 698 PVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASKSLFGSISSFS 756

Query: 668 GSSSAFVV 675
           GSSSAFVV
Sbjct: 758 GSSSAFVV 756

BLAST of Cucsat.G8367.T8 vs. ExPASy Swiss-Prot
Match: Q0JL46 (Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1 SV=1)

HSP 1 Score: 986.1 bits (2548), Expect = 2.0e-286
Identity = 470/676 (69.53%), Postives = 560/676 (82.84%), Query Frame = 0

Query: 8   VRKRYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQA 67
           ++ RYGDLY E NVAISGIH+HAGPGGYLQYVVYIVTSLGFVRQSF+V+VDGIE+SI++A
Sbjct: 113 LKARYGDLYNENNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVIVDGIEQSIVEA 172

Query: 68  HENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPV 127
           H NL PG I +NKG+L+DAGVNRSPSAYLNNPA ERSKY+Y+VDKEMTL+KF+DDE GPV
Sbjct: 173 HNNLRPGKIFVNKGDLLDAGVNRSPSAYLNNPAEERSKYEYNVDKEMTLIKFVDDELGPV 232

Query: 128 GTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKG--TGTLHHGESEADS----- 187
           G+FNWFATHGTSMSRTN+LISGDNKGAAARFMEDW +Q G    + H    +  S     
Sbjct: 233 GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWAEQMGLPKQSAHANSDDLRSLHKTS 292

Query: 188 -IPRRVSNIVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSA 247
            +PRRVS I+PE  +   +L++LA+S+++  GR      SI+ R+R+   Q ++P+FVSA
Sbjct: 293 VLPRRVSTIIPEPNEITDDLIQLASSYEASGGRRLAG-SSITRRIRST--QQNKPKFVSA 352

Query: 248 FCQSNCGDVSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQ 307
           FCQSNCGDVSPN LG FC+DT LPCDFNHSTC GKNELCYGRGPGYPDEFESTR+IG +Q
Sbjct: 353 FCQSNCGDVSPNVLGTFCIDTNLPCDFNHSTCNGKNELCYGRGPGYPDEFESTRVIGNRQ 412

Query: 308 FRKAVDLFSKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGT 367
           F KA DLF  ASE++ GK+D+RH+Y+DFS LEV ++   G  + VKTCPAAMGFAFAAGT
Sbjct: 413 FLKARDLFDSASEEIQGKIDYRHTYLDFSKLEVKVSTSAGGQQTVKTCPAAMGFAFAAGT 472

Query: 368 TDGPGAFDFKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSI 427
           TDGPGAFDF+QGD KGN FWKLVRN+LK PG +Q+ C SPKPILLDTGEMK PYDWAP+I
Sbjct: 473 TDGPGAFDFRQGDVKGNPFWKLVRNLLKTPGKDQVECHSPKPILLDTGEMKEPYDWAPAI 532

Query: 428 LPIQILRIGQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYS 487
           LP+Q++RIGQLVIL VPGEFTTMAGRRLRDAVKTVLT+G   EF+ N+HVV+AGLTN+YS
Sbjct: 533 LPVQMIRIGQLVILCVPGEFTTMAGRRLRDAVKTVLTSG-NSEFDKNIHVVLAGLTNSYS 592

Query: 488 QYVTTFEEYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQI 547
           QY+TTFEEY++QRYEGASTLYGPHTL AYIQEF+KLA ++I    V    QPPD+L +QI
Sbjct: 593 QYITTFEEYQIQRYEGASTLYGPHTLSAYIQEFQKLAMAMIANKEVPTNFQPPDMLDKQI 652

Query: 548 SLLPPVILDMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEI 607
            LLP V+ D TPLGV FGDV  DVP NS+F +G+ V  TF++ CPRNDL+T+GTFALVE 
Sbjct: 653 GLLPGVVFDSTPLGVKFGDVNSDVPGNSTFNKGSTVNATFYSACPRNDLLTDGTFALVEK 712

Query: 608 LQ-KNTWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSL 667
           L   N WVP YDDDD+ LRFKWSRPA LS++S+AT+EW +P+ A +GVYR+RHFGA+K +
Sbjct: 713 LDGNNNWVPVYDDDDWSLRFKWSRPARLSSRSFATLEWTVPEDAAAGVYRLRHFGASKPM 772

Query: 668 LGSIRHFTGSSSAFVV 675
            GS+RHFTG+S AF V
Sbjct: 773 FGSVRHFTGTSRAFAV 784

BLAST of Cucsat.G8367.T8 vs. ExPASy Swiss-Prot
Match: F4KHQ8 (Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 3.6e-272
Identity = 466/671 (69.45%), Postives = 541/671 (80.63%), Query Frame = 0

Query: 8   VRKRYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQA 67
           +++RYG+LYTE+NVAISG H+HAGPGGYLQY++Y+VTSLGFV QSFN LVDGIE+SIIQA
Sbjct: 101 LKQRYGELYTEENVAISGTHTHAGPGGYLQYILYLVTSLGFVHQSFNALVDGIEQSIIQA 160

Query: 68  HENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPV 127
           HENL PGSILINKGEL+DAGVNRSPSAYLNNPA ERSKY+YDVDKEMTL+KF+DD+WGPV
Sbjct: 161 HENLRPGSILINKGELLDAGVNRSPSAYLNNPAHERSKYEYDVDKEMTLVKFVDDQWGPV 220

Query: 128 GTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSN 187
                                       AR MEDWF+++        ES     PRRVS+
Sbjct: 221 ----------------------------ARIMEDWFERENGCRSVDVES-----PRRVSS 280

Query: 188 IVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGD 247
           I+ + Y   Q+L+E+A+S  S  G+  TR+ S++ RVR+  R AD+P+FVSAFCQ+NCGD
Sbjct: 281 IISDPY--DQDLMEMASSLLSTGGKTVTRMSSVARRVRSRFRHADKPRFVSAFCQTNCGD 340

Query: 248 VSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLF 307
           VSPN LGAFC+DTGLPC+FN STCGGKNE CYGRGPGYPDEFESTRIIGE+QF+KA DLF
Sbjct: 341 VSPNVLGAFCIDTGLPCEFNQSTCGGKNEQCYGRGPGYPDEFESTRIIGERQFKKAADLF 400

Query: 308 SKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFD 367
           +KASE++ GKVD+RH+YVDFS LEV++N Q G +EVVKTCPAAMGF FAAGTTDGPGAFD
Sbjct: 401 TKASEEIQGKVDYRHAYVDFSQLEVTINGQNGGSEVVKTCPAAMGFGFAAGTTDGPGAFD 460

Query: 368 FKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRI 427
           FKQGDD+GN FW+LVRN+LK P  EQ+ CQ PKPILLDTGEMK PYDWAPSILP+QILRI
Sbjct: 461 FKQGDDQGNPFWRLVRNLLKNPTEEQVRCQRPKPILLDTGEMKQPYDWAPSILPVQILRI 520

Query: 428 GQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAK-KEFNSNVHVVIAGLTNTYSQYVTTFE 487
           GQLVIL VPGEFTTMAGRRLRDAVKTVL  G+  +EF+    VVIAGLTN+YSQY+ TFE
Sbjct: 521 GQLVILCVPGEFTTMAGRRLRDAVKTVLKEGSNGREFS----VVIAGLTNSYSQYIATFE 580

Query: 488 EYKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVI 547
           EY++QRYEGASTLYGPHTL  YIQEFKKLA  L+      PGPQPPDLL +QISLL PV+
Sbjct: 581 EYQVQRYEGASTLYGPHTLSGYIQEFKKLANDLLSAQTTDPGPQPPDLLHKQISLLTPVV 640

Query: 548 LDMTPLGVSFGDVKFDVPSNSSFKRG-NLVKVTFWTGCPRNDLMTEGTFALVE--ILQKN 607
            DMTP+G +FGDV  DVP  S F++G ++V+V F +  PRNDLMTEGTFALVE  +  + 
Sbjct: 641 ADMTPIGTAFGDVTSDVPRLSKFRKGADIVRVQFRSANPRNDLMTEGTFALVERWLEGRE 700

Query: 608 TWVPAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIR 667
           TWVP YDDDDFCLRFKWSRP  LS QS ATIEWRIP+TA  GVYRI HFG+AK+ + SI 
Sbjct: 701 TWVPVYDDDDFCLRFKWSRPFKLSTQSTATIEWRIPETASPGVYRITHFGSAKTPISSIH 732

Query: 668 HFTGSSSAFVV 675
           HF+GSSSAFVV
Sbjct: 761 HFSGSSSAFVV 732

BLAST of Cucsat.G8367.T8 vs. ExPASy Swiss-Prot
Match: Q55G11 (Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 5.2e-146
Identity = 293/668 (43.86%), Postives = 390/668 (58.38%), Query Frame = 0

Query: 14  DLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQAHENLSP 73
           ++YTE NV +SG H+H+GP G+ QY +Y +TSLGF +++F+ + +GI ++I++AH+++ P
Sbjct: 125 NVYTEANVLLSGTHTHSGPAGFSQYALYGITSLGFYKKNFDTICNGIVQAIVKAHKSVQP 184

Query: 74  GSILINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPVGTFNWF 133
            ++    GEL +  +NRSP AY NNP  E++ Y  +VDK MT+L+  D    P    ++F
Sbjct: 185 ANMFTETGELWNTNINRSPFAYDNNPEEEKAMYDSNVDKNMTVLRIEDMNGNPFAAISFF 244

Query: 134 ATHGTSMSRTNALISGDNKGAAARFMEDWFKQ-KGTGTLHHGESEADSIPRRVSNIVPEV 193
           A H TSM+ TN LISGDNKG A+     W KQ  G GT                      
Sbjct: 245 AVHCTSMNNTNHLISGDNKGYASYL---WEKQVNGPGT---------------------- 304

Query: 194 YKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGDVSPNT 253
                                                 A +  FV+AF QSN GDVSPNT
Sbjct: 305 --------------------------------------AGKGPFVAAFGQSNEGDVSPNT 364

Query: 254 LGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYP-DEFESTRIIGEKQFRKAVDLFSKAS 313
            G  C D G PCD+  STC G+NE C+  GPG   D FEST+IIG  QF KA++LF+ AS
Sbjct: 365 RGPTCRD-GSPCDYKTSTCNGRNEECWSLGPGKDGDMFESTQIIGGNQFNKALELFNNAS 424

Query: 314 EQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFDFKQG 373
            Q++G V +RHS+V F+++ V      GV     TC  AMG++FAAGTTDGPGAF+F Q 
Sbjct: 425 IQVSGPVQYRHSWVQFTNVSVEPPYNSGVDNAT-TCRGAMGYSFAAGTTDGPGAFNFVQS 484

Query: 374 DD--KGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRIGQ 433
           D+   GN FW  + + +  P  +QI CQSPKPILLD G M  P  W P ++PIQI+ IGQ
Sbjct: 485 DNNTSGNPFWNFIGDFIAKPTPDQIRCQSPKPILLDVG-MVEPIPWVPDVMPIQIVTIGQ 544

Query: 434 LVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEEYK 493
           +V++ VPGEFTTM+GRRLR++V+ ++      E   N  V+IAGL+NTYS Y+ TFEEY+
Sbjct: 545 IVLVAVPGEFTTMSGRRLRNSVREII-----GESIENPIVLIAGLSNTYSGYIATFEEYQ 604

Query: 494 MQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVILDM 553
           +QRYEGAST++GPHTL +Y+QEF KLAQS++DGT V  GP P +L    +  LPPVI+D 
Sbjct: 605 VQRYEGASTVFGPHTLGSYMQEFGKLAQSIVDGTTVPAGPTPRNLTGHTLFFLPPVIVDA 664

Query: 554 TPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQ-KNTWVPA 613
            P    FG+V  DV  N ++     V   F+ G PRND M E +F  V++L   + W   
Sbjct: 665 APDFDDFGEVSIDV--NLNYSVNETVSCVFYGGNPRNDFMIESSFLSVDLLTGTDQWTTV 717

Query: 614 YDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKS--LLGSIRHFT 673
            DD D+  +FKW         S  TIEW I      G YRI H G AK      ++  + 
Sbjct: 725 LDDGDWDTKFKWKMHD--LGFSLITIEWVIAPDTTPGTYRITHSGFAKKNPFSSNLTPYQ 717

Query: 674 GSSSAFVV 675
           G S  FVV
Sbjct: 785 GISRNFVV 717

BLAST of Cucsat.G8367.T8 vs. NCBI nr
Match: XP_004153679.2 (neutral ceramidase 2 [Cucumis sativus] >KGN65777.1 hypothetical protein Csa_023365 [Cucumis sativus])

HSP 1 Score: 1351 bits (3497), Expect = 0.0
Identity = 665/668 (99.55%), Postives = 667/668 (99.85%), Query Frame = 0

Query: 8   VRKRYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQA 67
           ++ RYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQA
Sbjct: 112 LKARYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQA 171

Query: 68  HENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPV 127
           HENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPV
Sbjct: 172 HENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPV 231

Query: 128 GTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSN 187
           GTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSN
Sbjct: 232 GTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSN 291

Query: 188 IVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGD 247
           IVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGD
Sbjct: 292 IVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGD 351

Query: 248 VSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLF 307
           VSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLF
Sbjct: 352 VSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLF 411

Query: 308 SKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFD 367
           SKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFD
Sbjct: 412 SKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFD 471

Query: 368 FKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRI 427
           FKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRI
Sbjct: 472 FKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRI 531

Query: 428 GQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEE 487
           GQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEE
Sbjct: 532 GQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEE 591

Query: 488 YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVIL 547
           YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVIL
Sbjct: 592 YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVIL 651

Query: 548 DMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVP 607
           DMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVP
Sbjct: 652 DMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVP 711

Query: 608 AYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTG 667
           AYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTG
Sbjct: 712 AYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTG 771

Query: 668 SSSAFVVA 675
           SSSAFVVA
Sbjct: 772 SSSAFVVA 779

BLAST of Cucsat.G8367.T8 vs. NCBI nr
Match: XP_008457413.1 (PREDICTED: neutral ceramidase-like isoform X2 [Cucumis melo])

HSP 1 Score: 1293 bits (3346), Expect = 0.0
Identity = 634/667 (95.05%), Postives = 643/667 (96.40%), Query Frame = 0

Query: 8   VRKRYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQA 67
           V KRYGDLYTEKNVAISGIHSHAGPGGYLQY+VYIVTSLGFVRQSF+VLVDGIEKSIIQA
Sbjct: 16  VSKRYGDLYTEKNVAISGIHSHAGPGGYLQYIVYIVTSLGFVRQSFDVLVDGIEKSIIQA 75

Query: 68  HENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPV 127
           HENLSPGSILINKGELIDAGVNRSPSAYLNNPASE SKYKYDVDKEMTLLKFIDDEWGPV
Sbjct: 76  HENLSPGSILINKGELIDAGVNRSPSAYLNNPASECSKYKYDVDKEMTLLKFIDDEWGPV 135

Query: 128 GTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSN 187
           GTFNWFATHGTSMSRTN+LISGDNKGAAARFMEDWF+QKGTGTLH GESEADSIPRRVSN
Sbjct: 136 GTFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFEQKGTGTLHPGESEADSIPRRVSN 195

Query: 188 IVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGD 247
           I+PEVYKDKQELLE AASFQSQPGRPATR LS+SSRVRNVLRQADRPQFVSAFCQSNCGD
Sbjct: 196 IIPEVYKDKQELLERAASFQSQPGRPATRALSVSSRVRNVLRQADRPQFVSAFCQSNCGD 255

Query: 248 VSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLF 307
           VSPNTLGAFCLDT LPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGE+QFRKAVDLF
Sbjct: 256 VSPNTLGAFCLDTELPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLF 315

Query: 308 SKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFD 367
            KASE LTGKVDFRHSYVDFSHLEVSL KQGG  EVVKTCPAAMGFAFAAGTTDGPGAFD
Sbjct: 316 GKASEHLTGKVDFRHSYVDFSHLEVSLTKQGGAAEVVKTCPAAMGFAFAAGTTDGPGAFD 375

Query: 368 FKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRI 427
           FKQGDDKGN FWKLVRN+LKAPGNEQISCQSPKPILLDTGEMK PYDWAP+ILPIQILRI
Sbjct: 376 FKQGDDKGNPFWKLVRNLLKAPGNEQISCQSPKPILLDTGEMKIPYDWAPTILPIQILRI 435

Query: 428 GQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEE 487
           GQLVIL VPGEFTTMAGRRLRDAVKTVLTTGA KEFNSNVHVVIAGLTNTYSQYVTTFEE
Sbjct: 436 GQLVILSVPGEFTTMAGRRLRDAVKTVLTTGANKEFNSNVHVVIAGLTNTYSQYVTTFEE 495

Query: 488 YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVIL 547
           YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDG PV PGPQPPDLL RQISLLPPVIL
Sbjct: 496 YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGRPVVPGPQPPDLLDRQISLLPPVIL 555

Query: 548 DMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVP 607
           DMTP GVSFGDVK DVP NSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVP
Sbjct: 556 DMTPPGVSFGDVKSDVPRNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVP 615

Query: 608 AYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTG 667
           AYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQT VSGVYRIRHFGAAKS  GSIRHFTG
Sbjct: 616 AYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTVVSGVYRIRHFGAAKSFFGSIRHFTG 675

Query: 668 SSSAFVV 674
           SSSAFVV
Sbjct: 676 SSSAFVV 682

BLAST of Cucsat.G8367.T8 vs. NCBI nr
Match: XP_008457412.1 (PREDICTED: neutral ceramidase-like isoform X1 [Cucumis melo])

HSP 1 Score: 1291 bits (3340), Expect = 0.0
Identity = 632/667 (94.75%), Postives = 643/667 (96.40%), Query Frame = 0

Query: 8   VRKRYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQA 67
           ++ RYGDLYTEKNVAISGIHSHAGPGGYLQY+VYIVTSLGFVRQSF+VLVDGIEKSIIQA
Sbjct: 112 LKARYGDLYTEKNVAISGIHSHAGPGGYLQYIVYIVTSLGFVRQSFDVLVDGIEKSIIQA 171

Query: 68  HENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPV 127
           HENLSPGSILINKGELIDAGVNRSPSAYLNNPASE SKYKYDVDKEMTLLKFIDDEWGPV
Sbjct: 172 HENLSPGSILINKGELIDAGVNRSPSAYLNNPASECSKYKYDVDKEMTLLKFIDDEWGPV 231

Query: 128 GTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSN 187
           GTFNWFATHGTSMSRTN+LISGDNKGAAARFMEDWF+QKGTGTLH GESEADSIPRRVSN
Sbjct: 232 GTFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFEQKGTGTLHPGESEADSIPRRVSN 291

Query: 188 IVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGD 247
           I+PEVYKDKQELLE AASFQSQPGRPATR LS+SSRVRNVLRQADRPQFVSAFCQSNCGD
Sbjct: 292 IIPEVYKDKQELLERAASFQSQPGRPATRALSVSSRVRNVLRQADRPQFVSAFCQSNCGD 351

Query: 248 VSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLF 307
           VSPNTLGAFCLDT LPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGE+QFRKAVDLF
Sbjct: 352 VSPNTLGAFCLDTELPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLF 411

Query: 308 SKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFD 367
            KASE LTGKVDFRHSYVDFSHLEVSL KQGG  EVVKTCPAAMGFAFAAGTTDGPGAFD
Sbjct: 412 GKASEHLTGKVDFRHSYVDFSHLEVSLTKQGGAAEVVKTCPAAMGFAFAAGTTDGPGAFD 471

Query: 368 FKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRI 427
           FKQGDDKGN FWKLVRN+LKAPGNEQISCQSPKPILLDTGEMK PYDWAP+ILPIQILRI
Sbjct: 472 FKQGDDKGNPFWKLVRNLLKAPGNEQISCQSPKPILLDTGEMKIPYDWAPTILPIQILRI 531

Query: 428 GQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEE 487
           GQLVIL VPGEFTTMAGRRLRDAVKTVLTTGA KEFNSNVHVVIAGLTNTYSQYVTTFEE
Sbjct: 532 GQLVILSVPGEFTTMAGRRLRDAVKTVLTTGANKEFNSNVHVVIAGLTNTYSQYVTTFEE 591

Query: 488 YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVIL 547
           YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDG PV PGPQPPDLL RQISLLPPVIL
Sbjct: 592 YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGRPVVPGPQPPDLLDRQISLLPPVIL 651

Query: 548 DMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVP 607
           DMTP GVSFGDVK DVP NSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVP
Sbjct: 652 DMTPPGVSFGDVKSDVPRNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVP 711

Query: 608 AYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTG 667
           AYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQT VSGVYRIRHFGAAKS  GSIRHFTG
Sbjct: 712 AYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTVVSGVYRIRHFGAAKSFFGSIRHFTG 771

Query: 668 SSSAFVV 674
           SSSAFVV
Sbjct: 772 SSSAFVV 778

BLAST of Cucsat.G8367.T8 vs. NCBI nr
Match: XP_038892835.1 (neutral ceramidase 2-like [Benincasa hispida])

HSP 1 Score: 1264 bits (3271), Expect = 0.0
Identity = 615/669 (91.93%), Postives = 648/669 (96.86%), Query Frame = 0

Query: 8   VRKRYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQA 67
           ++ RYGDLYTEKNVAISGIH+HAGPGGYLQYVVYIVTSLGFVRQSF+VLVDGIEKSIIQA
Sbjct: 112 LKARYGDLYTEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQA 171

Query: 68  HENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPV 127
           HENL PGSILINKGELIDAGVNRSPSAYLNNPASERS+YKYDVDKEMTLLKF+DDEWGPV
Sbjct: 172 HENLLPGSILINKGELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPV 231

Query: 128 GTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSN 187
           G+FNWFATHGTSMSRTN+LISGDNKGAAARFMEDWF+QKGTGT+H GESE DSIPRRVSN
Sbjct: 232 GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFEQKGTGTMHPGESEVDSIPRRVSN 291

Query: 188 IVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGD 247
           I+PEV+K+KQELLE AASFQSQPGRPATR+LS+S RVRNVLRQA+RPQFVSAFCQSNCGD
Sbjct: 292 IIPEVFKNKQELLERAASFQSQPGRPATRILSVSRRVRNVLRQANRPQFVSAFCQSNCGD 351

Query: 248 VSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLF 307
           VSPNTLGAFC+DTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGE+QFRKAVDLF
Sbjct: 352 VSPNTLGAFCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLF 411

Query: 308 SKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFD 367
           SKASEQLTGKVDFRHSYVDFS LEVSL+KQGG TEV++TCPAAMGFAFAAGTTDGPGAFD
Sbjct: 412 SKASEQLTGKVDFRHSYVDFSQLEVSLSKQGGATEVIRTCPAAMGFAFAAGTTDGPGAFD 471

Query: 368 FKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRI 427
           FKQGDDKGNAFWKLVRN+LKAPGNEQI+CQSPKPILLDTGEMK PYDWAPSILPIQILRI
Sbjct: 472 FKQGDDKGNAFWKLVRNLLKAPGNEQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRI 531

Query: 428 GQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEE 487
           GQLVIL VPGEFTTMAGRRLRDAVKTVLTTGAK+EFN NVHVVIAGLTNTYSQYVTTFEE
Sbjct: 532 GQLVILGVPGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEE 591

Query: 488 YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVIL 547
           Y+MQRYEGASTLYGPHTL+AYIQEFKKLAQSLIDG PV PGPQPPDLLARQISLL PVI+
Sbjct: 592 YEMQRYEGASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLARQISLLTPVIM 651

Query: 548 DMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQK-NTWV 607
           D TP+GVSFGDVK DVP NS+FKRGNLV V+FW+GCPRNDLMTEGTFALVEILQK +TWV
Sbjct: 652 DATPIGVSFGDVKSDVPLNSTFKRGNLVTVSFWSGCPRNDLMTEGTFALVEILQKKDTWV 711

Query: 608 PAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFT 667
           PAYDDDDFCLRFKWSRP+ LS QSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFT
Sbjct: 712 PAYDDDDFCLRFKWSRPSRLSPQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFT 771

Query: 668 GSSSAFVVA 675
           GSSSAFVVA
Sbjct: 772 GSSSAFVVA 780

BLAST of Cucsat.G8367.T8 vs. NCBI nr
Match: KAG6583392.1 (Neutral ceramidase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1232 bits (3187), Expect = 0.0
Identity = 599/669 (89.54%), Postives = 637/669 (95.22%), Query Frame = 0

Query: 8   VRKRYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQA 67
           ++ RYGDLYTE+NVAISGIH+HAGPGGYLQYVVYIVTSLGFVRQSF+VLVDGIEKSIIQA
Sbjct: 112 LKARYGDLYTEENVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQA 171

Query: 68  HENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPV 127
           HENL PGSIL+NKGELIDAGVNRSPSAYLNNPASER++YKYDVDKEMTLLKFIDD+WGPV
Sbjct: 172 HENLLPGSILVNKGELIDAGVNRSPSAYLNNPASERNRYKYDVDKEMTLLKFIDDQWGPV 231

Query: 128 GTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSN 187
           G+FNWFATHGTSMSRTN+LISGDNKGAAARFMEDWF QKG GTL  G+SEADSIPRRVSN
Sbjct: 232 GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFVQKGIGTLQTGKSEADSIPRRVSN 291

Query: 188 IVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGD 247
           I+PE YKD+QELL  AASFQS+PGRPATR+LS+S RVRNVLRQADRPQFVSAFCQSNCGD
Sbjct: 292 IIPEAYKDEQELLARAASFQSRPGRPATRILSVSRRVRNVLRQADRPQFVSAFCQSNCGD 351

Query: 248 VSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLF 307
           VSPNTLGAFC+DTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGE+QFRKAVDLF
Sbjct: 352 VSPNTLGAFCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLF 411

Query: 308 SKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFD 367
           SKASEQL G VD+RHSYVDFS LEVS+ K GG TEVVKTCPAAMGFAFAAGTTDGPGAFD
Sbjct: 412 SKASEQLKGTVDYRHSYVDFSQLEVSVTKAGGATEVVKTCPAAMGFAFAAGTTDGPGAFD 471

Query: 368 FKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRI 427
           FKQGDDKGNAFWKLVRN+LK PG++QI+CQSPKPILLDTGEMK PYDWAPSILPIQILRI
Sbjct: 472 FKQGDDKGNAFWKLVRNLLKTPGSKQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRI 531

Query: 428 GQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEE 487
           GQLVIL VPGEFTTMAGRRLRDAVKTVLTTGAK+EFN NVHVVIAGLTNTYSQYVTTFEE
Sbjct: 532 GQLVILGVPGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEE 591

Query: 488 YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVIL 547
           Y+MQRYEGASTLYGPHTL+AYIQEF+KLAQSLIDG PV PGPQPPDLLA+QISLL PV++
Sbjct: 592 YEMQRYEGASTLYGPHTLDAYIQEFRKLAQSLIDGRPVEPGPQPPDLLAKQISLLTPVVM 651

Query: 548 DMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQK-NTWV 607
           D TP GVSFGDVK DVP NS+FKRG+LV VTFW+GCPRNDLMTEGTFALVEILQK +TWV
Sbjct: 652 DATPFGVSFGDVKSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWV 711

Query: 608 PAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFT 667
           P+YDDDDFCLRFKWSRP+ LS QS ATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFT
Sbjct: 712 PSYDDDDFCLRFKWSRPSRLSPQSLATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFT 771

Query: 668 GSSSAFVVA 675
           GSSSAFVVA
Sbjct: 772 GSSSAFVVA 780

BLAST of Cucsat.G8367.T8 vs. ExPASy TrEMBL
Match: A0A0A0LY63 (Neutral ceramidase OS=Cucumis sativus OX=3659 GN=Csa_1G527930 PE=3 SV=1)

HSP 1 Score: 1351 bits (3497), Expect = 0.0
Identity = 665/668 (99.55%), Postives = 667/668 (99.85%), Query Frame = 0

Query: 8   VRKRYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQA 67
           ++ RYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQA
Sbjct: 112 LKARYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQA 171

Query: 68  HENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPV 127
           HENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPV
Sbjct: 172 HENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPV 231

Query: 128 GTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSN 187
           GTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSN
Sbjct: 232 GTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSN 291

Query: 188 IVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGD 247
           IVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGD
Sbjct: 292 IVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGD 351

Query: 248 VSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLF 307
           VSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLF
Sbjct: 352 VSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLF 411

Query: 308 SKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFD 367
           SKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFD
Sbjct: 412 SKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFD 471

Query: 368 FKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRI 427
           FKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRI
Sbjct: 472 FKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRI 531

Query: 428 GQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEE 487
           GQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEE
Sbjct: 532 GQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEE 591

Query: 488 YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVIL 547
           YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVIL
Sbjct: 592 YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVIL 651

Query: 548 DMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVP 607
           DMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVP
Sbjct: 652 DMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVP 711

Query: 608 AYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTG 667
           AYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTG
Sbjct: 712 AYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTG 771

Query: 668 SSSAFVVA 675
           SSSAFVVA
Sbjct: 772 SSSAFVVA 779

BLAST of Cucsat.G8367.T8 vs. ExPASy TrEMBL
Match: A0A1S3C650 (Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103497106 PE=3 SV=1)

HSP 1 Score: 1293 bits (3346), Expect = 0.0
Identity = 634/667 (95.05%), Postives = 643/667 (96.40%), Query Frame = 0

Query: 8   VRKRYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQA 67
           V KRYGDLYTEKNVAISGIHSHAGPGGYLQY+VYIVTSLGFVRQSF+VLVDGIEKSIIQA
Sbjct: 16  VSKRYGDLYTEKNVAISGIHSHAGPGGYLQYIVYIVTSLGFVRQSFDVLVDGIEKSIIQA 75

Query: 68  HENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPV 127
           HENLSPGSILINKGELIDAGVNRSPSAYLNNPASE SKYKYDVDKEMTLLKFIDDEWGPV
Sbjct: 76  HENLSPGSILINKGELIDAGVNRSPSAYLNNPASECSKYKYDVDKEMTLLKFIDDEWGPV 135

Query: 128 GTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSN 187
           GTFNWFATHGTSMSRTN+LISGDNKGAAARFMEDWF+QKGTGTLH GESEADSIPRRVSN
Sbjct: 136 GTFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFEQKGTGTLHPGESEADSIPRRVSN 195

Query: 188 IVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGD 247
           I+PEVYKDKQELLE AASFQSQPGRPATR LS+SSRVRNVLRQADRPQFVSAFCQSNCGD
Sbjct: 196 IIPEVYKDKQELLERAASFQSQPGRPATRALSVSSRVRNVLRQADRPQFVSAFCQSNCGD 255

Query: 248 VSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLF 307
           VSPNTLGAFCLDT LPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGE+QFRKAVDLF
Sbjct: 256 VSPNTLGAFCLDTELPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLF 315

Query: 308 SKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFD 367
            KASE LTGKVDFRHSYVDFSHLEVSL KQGG  EVVKTCPAAMGFAFAAGTTDGPGAFD
Sbjct: 316 GKASEHLTGKVDFRHSYVDFSHLEVSLTKQGGAAEVVKTCPAAMGFAFAAGTTDGPGAFD 375

Query: 368 FKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRI 427
           FKQGDDKGN FWKLVRN+LKAPGNEQISCQSPKPILLDTGEMK PYDWAP+ILPIQILRI
Sbjct: 376 FKQGDDKGNPFWKLVRNLLKAPGNEQISCQSPKPILLDTGEMKIPYDWAPTILPIQILRI 435

Query: 428 GQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEE 487
           GQLVIL VPGEFTTMAGRRLRDAVKTVLTTGA KEFNSNVHVVIAGLTNTYSQYVTTFEE
Sbjct: 436 GQLVILSVPGEFTTMAGRRLRDAVKTVLTTGANKEFNSNVHVVIAGLTNTYSQYVTTFEE 495

Query: 488 YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVIL 547
           YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDG PV PGPQPPDLL RQISLLPPVIL
Sbjct: 496 YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGRPVVPGPQPPDLLDRQISLLPPVIL 555

Query: 548 DMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVP 607
           DMTP GVSFGDVK DVP NSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVP
Sbjct: 556 DMTPPGVSFGDVKSDVPRNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVP 615

Query: 608 AYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTG 667
           AYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQT VSGVYRIRHFGAAKS  GSIRHFTG
Sbjct: 616 AYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTVVSGVYRIRHFGAAKSFFGSIRHFTG 675

Query: 668 SSSAFVV 674
           SSSAFVV
Sbjct: 676 SSSAFVV 682

BLAST of Cucsat.G8367.T8 vs. ExPASy TrEMBL
Match: A0A1S3C5F7 (Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103497106 PE=3 SV=1)

HSP 1 Score: 1291 bits (3340), Expect = 0.0
Identity = 632/667 (94.75%), Postives = 643/667 (96.40%), Query Frame = 0

Query: 8   VRKRYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQA 67
           ++ RYGDLYTEKNVAISGIHSHAGPGGYLQY+VYIVTSLGFVRQSF+VLVDGIEKSIIQA
Sbjct: 112 LKARYGDLYTEKNVAISGIHSHAGPGGYLQYIVYIVTSLGFVRQSFDVLVDGIEKSIIQA 171

Query: 68  HENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPV 127
           HENLSPGSILINKGELIDAGVNRSPSAYLNNPASE SKYKYDVDKEMTLLKFIDDEWGPV
Sbjct: 172 HENLSPGSILINKGELIDAGVNRSPSAYLNNPASECSKYKYDVDKEMTLLKFIDDEWGPV 231

Query: 128 GTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSN 187
           GTFNWFATHGTSMSRTN+LISGDNKGAAARFMEDWF+QKGTGTLH GESEADSIPRRVSN
Sbjct: 232 GTFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFEQKGTGTLHPGESEADSIPRRVSN 291

Query: 188 IVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGD 247
           I+PEVYKDKQELLE AASFQSQPGRPATR LS+SSRVRNVLRQADRPQFVSAFCQSNCGD
Sbjct: 292 IIPEVYKDKQELLERAASFQSQPGRPATRALSVSSRVRNVLRQADRPQFVSAFCQSNCGD 351

Query: 248 VSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLF 307
           VSPNTLGAFCLDT LPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGE+QFRKAVDLF
Sbjct: 352 VSPNTLGAFCLDTELPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLF 411

Query: 308 SKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFD 367
            KASE LTGKVDFRHSYVDFSHLEVSL KQGG  EVVKTCPAAMGFAFAAGTTDGPGAFD
Sbjct: 412 GKASEHLTGKVDFRHSYVDFSHLEVSLTKQGGAAEVVKTCPAAMGFAFAAGTTDGPGAFD 471

Query: 368 FKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRI 427
           FKQGDDKGN FWKLVRN+LKAPGNEQISCQSPKPILLDTGEMK PYDWAP+ILPIQILRI
Sbjct: 472 FKQGDDKGNPFWKLVRNLLKAPGNEQISCQSPKPILLDTGEMKIPYDWAPTILPIQILRI 531

Query: 428 GQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEE 487
           GQLVIL VPGEFTTMAGRRLRDAVKTVLTTGA KEFNSNVHVVIAGLTNTYSQYVTTFEE
Sbjct: 532 GQLVILSVPGEFTTMAGRRLRDAVKTVLTTGANKEFNSNVHVVIAGLTNTYSQYVTTFEE 591

Query: 488 YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVIL 547
           YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDG PV PGPQPPDLL RQISLLPPVIL
Sbjct: 592 YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGRPVVPGPQPPDLLDRQISLLPPVIL 651

Query: 548 DMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVP 607
           DMTP GVSFGDVK DVP NSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVP
Sbjct: 652 DMTPPGVSFGDVKSDVPRNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQKNTWVP 711

Query: 608 AYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFTG 667
           AYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQT VSGVYRIRHFGAAKS  GSIRHFTG
Sbjct: 712 AYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTVVSGVYRIRHFGAAKSFFGSIRHFTG 771

Query: 668 SSSAFVV 674
           SSSAFVV
Sbjct: 772 SSSAFVV 778

BLAST of Cucsat.G8367.T8 vs. ExPASy TrEMBL
Match: A0A6J1DAR9 (Neutral ceramidase OS=Momordica charantia OX=3673 GN=LOC111018960 PE=3 SV=1)

HSP 1 Score: 1229 bits (3181), Expect = 0.0
Identity = 596/669 (89.09%), Postives = 634/669 (94.77%), Query Frame = 0

Query: 8   VRKRYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQA 67
           ++ RYGDLYTEKNVAISGIH+HAGPGGYLQYVVYIVTSLGFVRQSF+VLVDGIEKSIIQA
Sbjct: 112 LKARYGDLYTEKNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQA 171

Query: 68  HENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPV 127
           HENL PGS+LIN GELIDAGVNRSPSAYLNNPASERS+YKYDVDKEMTLLKF+DDEWGPV
Sbjct: 172 HENLLPGSVLINTGELIDAGVNRSPSAYLNNPASERSRYKYDVDKEMTLLKFVDDEWGPV 231

Query: 128 GTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSN 187
           G+FNWFATHGTSMSRTN+LISGDNKGAAARFMEDWF QKG G+LH G+S ADSIPRRVSN
Sbjct: 232 GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFDQKGIGSLHPGQSVADSIPRRVSN 291

Query: 188 IVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGD 247
           I+PE YK+KQELLE AASFQS+PGRPATR+LS++ RVRNVLRQADRPQFVSAFCQSNCGD
Sbjct: 292 IIPEAYKNKQELLERAASFQSRPGRPATRILSVARRVRNVLRQADRPQFVSAFCQSNCGD 351

Query: 248 VSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLF 307
           VSPN LGAFC+DTGLPCDFNHSTCGGKNELCYGRGPGYPDEFES RIIGE+QFRKAVDLF
Sbjct: 352 VSPNVLGAFCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESARIIGERQFRKAVDLF 411

Query: 308 SKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFD 367
           +KASE+LTGKVD+RHSYVDFS LEVSL KQGG TEVVKTCPAAMGFAFAAGTTDGPGAFD
Sbjct: 412 NKASEKLTGKVDYRHSYVDFSQLEVSLLKQGGATEVVKTCPAAMGFAFAAGTTDGPGAFD 471

Query: 368 FKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRI 427
           FKQGDDKGN FWKLVRN+L+APG +Q++CQSPKPILLDTGEMK PYDWAPSILPIQILRI
Sbjct: 472 FKQGDDKGNPFWKLVRNLLRAPGEQQVTCQSPKPILLDTGEMKAPYDWAPSILPIQILRI 531

Query: 428 GQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEE 487
           GQLVIL VPGEFTTMAGRRLR+AVKTVLTTGAK+EFN NVH+VIAGLTNTYSQYVTTFEE
Sbjct: 532 GQLVILGVPGEFTTMAGRRLRNAVKTVLTTGAKREFNGNVHIVIAGLTNTYSQYVTTFEE 591

Query: 488 YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVIL 547
           Y+MQRYEGASTLYGPHTL+AYIQEFKKLAQSLIDG PV PGPQPPDLL+RQISLLPPVI+
Sbjct: 592 YEMQRYEGASTLYGPHTLDAYIQEFKKLAQSLIDGRPVEPGPQPPDLLSRQISLLPPVIM 651

Query: 548 DMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQK-NTWV 607
           D TPLGVSFGDVK DVP NS+FKRGNLV VTFW+GCPRNDLMTEGTFALVEILQK +TWV
Sbjct: 652 DATPLGVSFGDVKSDVPLNSTFKRGNLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWV 711

Query: 608 PAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFT 667
           P YDDDDFCL FKW RP   S QSYATIEWRIPQ+AVSGVYRIRHFGAAKSL GSIRHFT
Sbjct: 712 PVYDDDDFCLFFKWLRPFRYSTQSYATIEWRIPQSAVSGVYRIRHFGAAKSLFGSIRHFT 771

Query: 668 GSSSAFVVA 675
           GSSSAFVVA
Sbjct: 772 GSSSAFVVA 780

BLAST of Cucsat.G8367.T8 vs. ExPASy TrEMBL
Match: A0A6J1HIT9 (Neutral ceramidase OS=Cucurbita moschata OX=3662 GN=LOC111464833 PE=3 SV=1)

HSP 1 Score: 1229 bits (3179), Expect = 0.0
Identity = 597/669 (89.24%), Postives = 636/669 (95.07%), Query Frame = 0

Query: 8   VRKRYGDLYTEKNVAISGIHSHAGPGGYLQYVVYIVTSLGFVRQSFNVLVDGIEKSIIQA 67
           ++ RYGDLYTE+NVAISGIH+HAGPGGYLQYVVYIVTSLGFVRQSF+VLVDGIEKSIIQA
Sbjct: 112 LKARYGDLYTEENVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVLVDGIEKSIIQA 171

Query: 68  HENLSPGSILINKGELIDAGVNRSPSAYLNNPASERSKYKYDVDKEMTLLKFIDDEWGPV 127
           HENL PGSIL+NKGELIDAGVNRSPSAYLNNPASER++YKYDVDKEMTLLKFIDD+WGPV
Sbjct: 172 HENLLPGSILVNKGELIDAGVNRSPSAYLNNPASERNRYKYDVDKEMTLLKFIDDQWGPV 231

Query: 128 GTFNWFATHGTSMSRTNALISGDNKGAAARFMEDWFKQKGTGTLHHGESEADSIPRRVSN 187
           G+FNWFATHGTSMSRTN+LISGDNKGAAARFMEDWF QKG GTL  G+SEADSIPRRVSN
Sbjct: 232 GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFVQKGIGTLQTGKSEADSIPRRVSN 291

Query: 188 IVPEVYKDKQELLELAASFQSQPGRPATRVLSISSRVRNVLRQADRPQFVSAFCQSNCGD 247
           I+PE Y D+QELL  AASFQS+PGRPATR+LS+S RVRNVLRQADRPQFVSAFCQSNCGD
Sbjct: 292 IIPEAYNDEQELLARAASFQSRPGRPATRILSVSRRVRNVLRQADRPQFVSAFCQSNCGD 351

Query: 248 VSPNTLGAFCLDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGEKQFRKAVDLF 307
           VSPNTLGAFC+DTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGE+QFRKAVDLF
Sbjct: 352 VSPNTLGAFCIDTGLPCDFNHSTCGGKNELCYGRGPGYPDEFESTRIIGERQFRKAVDLF 411

Query: 308 SKASEQLTGKVDFRHSYVDFSHLEVSLNKQGGVTEVVKTCPAAMGFAFAAGTTDGPGAFD 367
           SKASEQL G VD+RHSYVDFS LEVS+ K GG TEVVKTCPAAMGFAFAAGTTDGPGAFD
Sbjct: 412 SKASEQLKGTVDYRHSYVDFSQLEVSVTKAGGATEVVKTCPAAMGFAFAAGTTDGPGAFD 471

Query: 368 FKQGDDKGNAFWKLVRNVLKAPGNEQISCQSPKPILLDTGEMKTPYDWAPSILPIQILRI 427
           FKQGDDKGNAFWKLVRN+LK PG++QI+CQSPKPILLDTGEMK PYDWAPSILPIQILRI
Sbjct: 472 FKQGDDKGNAFWKLVRNLLKTPGSKQIACQSPKPILLDTGEMKIPYDWAPSILPIQILRI 531

Query: 428 GQLVILDVPGEFTTMAGRRLRDAVKTVLTTGAKKEFNSNVHVVIAGLTNTYSQYVTTFEE 487
           GQLVIL VPGEFTTMAGRRLRDAVKTVLTTGAK+EFN NVHVVIAGLTNTYSQYVTTFEE
Sbjct: 532 GQLVILGVPGEFTTMAGRRLRDAVKTVLTTGAKREFNGNVHVVIAGLTNTYSQYVTTFEE 591

Query: 488 YKMQRYEGASTLYGPHTLEAYIQEFKKLAQSLIDGTPVAPGPQPPDLLARQISLLPPVIL 547
           Y+MQRYEGASTLYGPHTL+AYIQEF+KLAQSLIDG PV PGPQPPDLLA+QISLL PV++
Sbjct: 592 YEMQRYEGASTLYGPHTLDAYIQEFRKLAQSLIDGRPVEPGPQPPDLLAKQISLLTPVVM 651

Query: 548 DMTPLGVSFGDVKFDVPSNSSFKRGNLVKVTFWTGCPRNDLMTEGTFALVEILQK-NTWV 607
           D TP GVSFGDV+ DVP NS+FKRG+LV VTFW+GCPRNDLMTEGTFALVEILQK +TWV
Sbjct: 652 DATPFGVSFGDVRSDVPLNSTFKRGDLVTVTFWSGCPRNDLMTEGTFALVEILQKKDTWV 711

Query: 608 PAYDDDDFCLRFKWSRPAPLSAQSYATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFT 667
           P+YDDDDFCLRFKWSRP+ LS QS ATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFT
Sbjct: 712 PSYDDDDFCLRFKWSRPSRLSPQSLATIEWRIPQTAVSGVYRIRHFGAAKSLLGSIRHFT 771

Query: 668 GSSSAFVVA 675
           GSSSAFVVA
Sbjct: 772 GSSSAFVVA 780