Cucsat.G7170 (gene) Cucumber (B10) v3

Overview
NameCucsat.G7170
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
Locationctg1528: 2816375 .. 2822339 (-)
RNA-Seq ExpressionCucsat.G7170
SyntenyCucsat.G7170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCATTGAGTTCTACTGACATTTCATTTTTATATTATTTTCTTGTTAATATGTAAAAGAGCAATGGCATCCCACATTGTTGGTTATCCTCGCATTGGTCCGAAGAGAGAACTTAAATTTGCCTTGGAGTCTTTCTGGGATGGGAAGAGCAGTGCTGATGACTTGCAGAAAGTGGCTGCTGATCTCAGGTCATCTATTTGGAAGCAGATGGCTGAAGCTGGAATCAAGTTTATTCCAAGCAACACTTTTTCGTATTATGATCAAGTGCTTGACACTGCAACTATGCTCGGAGCTGTTCCGTCAAGATATGGATGGAACGGTGGTGAGATTGGATTTGACATCTACTTCTCCATGGCTAGAGGGAACGCTTCAGTTCCTGCCATGGAAATGACGAAGTGGTTCGATACCAATTAGTAAGTGAAAAACTCTATGCATTTCCCTACATTTTTATGATAATACTATTTGTAAAACTTCTACTAGTCAATGATTTGAGGAATGTTAACGATGTGTGTTCTGTTACAGCCATTACATTGTGCCCGAGCTGAGTCCAGAAGATAAATTTACTTATGCATCTCACAAAGCAGTAAACGAGTTCAAGGAAGCTAAAGCTGTAAGATTTCTTATTAAAAGAAATTAGGGAAAAAAAAAAAAAACACTTAAAAATGAACTTATTTTCTGAGTTTGGATAATGGTGGTAGATTCAAGTTTCATGTGAATAAGATTAATTCAAGGAAGGATGTACTTAACTGGTTTGCACTTGTAATTTTTTTTTAATGTCCACCAAGAAAAAGTTTAAGAATTTTCTTGCCCTATGAACAGTAGTACAAGTTAACTTGAATCATCTATCCTTGAACTTGACAACAAACATCCTTTCCATTTCCAGTTTGATTAATTTTTTGCTTTCTTTGTCGTCTGTTAGTTCTTCTGATTCAGTGTTTTAATTTATTATTTCAATTTTTTTTTTATCATTTGACCTGCCCTACTGATGCATTTAACTTCTTTTATACTCCATCTTATATGATATTATATTTCTTATATTTAAAGAGGCATCTTGCTTTTATCATGCGACAGAACCTTATTTTTTAGCCATTCATAATAACAAGCAAGATGCCTTATTCGGACTGACATGCCTTTCAATTTAAGAATTGTTTCAAGTGAAGAATATTTGAATAAATGCCTGGATAGTAAATTATATTCTATGAGAATTTTATGATATTTGAAGTTCAGTCATGAAATTTCAATATGAGCATGTTAAGCATTTTTATTACCTTTTTTCCTTTACACTCCCACTTTTCTGTGCAGCTCGGAGTTGAGACTGTCCCGGTTCTTGTAGGCCCTGTATCCTTCTTGTTGCTTTCAAAACCAGCCAAGGGAGTTGAGAAATCCTTCTCACTTCTCTCCCTAATCGACAAGATTCTTCCTGTTTATAAGTCAGTATTTCTTTTCCTCTTTCCAAAGTTCGTCTAGTTTACGTATTAACCATTTCATTTCAGTTCCACTATCTTCTTATCAACTCTTATTTGTACCTGCAGGGAAGTTGTTTCAGAGCTGAAGGCTGCTGGTGCCACTTGGATTCAATTTGATGAGCCTACACTTGTGAAAGATCTTGACGCGCACCACTTACAAGCATTTGCTCAAGCCTACTCAGAGCTGGAATCCACTTTATCTGGTTTAAATGTTCTGATTGAGACATATTTTGCTGATGTTCCTGCTGAGGCATACAAAACTCTCACGTCTTTGAAGGGTGTCTCTGGTTATGGATTCGATCTAGTTCGTGGAACACAGACACTTGATCTGATCAAAGGTGGATTTCCTTCAGGCAAATATCTCTTTGCCGGAGTGGTTGATGGAAGAAACATATGGTCAAATGACCTTCAAGCTTCCATAACTACCTTGGAGGCGTTGGAAAACGTTGTTGGAAAAGGTGCTTGTTTCTTAACGTGCTAGCACATTTTATATTAGCGTGGATACATTTTTAGACTATAAACAGTGGTAATATATGCTGTTCTTCTGTCCTTTACAGAAAAAATTGTCGTCTCCACTTCCTGCTCTCTTCTTCACACTGCTGTTGATCTGGTGAACGAAACAAAACTAGATGATGAAATTAAGTCCTGGCTTGCTTTTGCCGCTCAGAAAGTTGTTGAAGTTAATGCCTTGGCTAAGGCACTCTCTGGACATAAAGATGAGGTACACGAAGCAACCTTATTTTTGACCTTGCAATAGGAACTTAGGGGCAGGGTGTTGTACCTTAAATCAAACAATGTAGCCTTAGTGCATAGTCTTTGTAGACTCATGGTACAAACGAAACATGTGTGTGTGTATATAATAAAATAAATGAAGCTTGACTTATTTGACATGAAATGGAAATCTATTGTTGCGGAACTGAAGATACTAAAATTTGAGCTTATAACTTTTTTCCTTTTAGGCAAAGAGAAAAATGAGCTTCTAACTGTCGCTTCTTCTATTCCAGGCATATTTTTCTTCCAATGCTGCTGCTCAAGCTTCAAGGAAATCATCCCCAAGAGTGACAAATGAGGCAGTCCAAAAAGCTGTATAAATTATTTGATGTACTAATATCATTTTGTAGGATCTCTCTATATTCTTGCATATCGTGTGCTTAACCTTCTATGTCGACTTTACTTAGGCTGCTGCTCTGAAGGGCTCTGACCACCGTCGGTCAACAAATGTAAGTGCCAGGCTGGACGCTCAGCAGAAGAAGTTGAACCTTCCAATGCTGCCTACAACAACAATTGGATCCTTCCCTCAAACTATGGATCTTAGAAGAGTGCGACGTGAATTCAAAGCAAAAAAGTATGCCTTTTTGTCTTCAGATATAATTGTTCGTCCTCAAAAAAGTACATTGTTGTTAATTTTCTAATACACTTGCATCCTGTTCCTTTAGAATCTCTGAGGAAGACTATGTCAGCTCCATCAAGGAGGAAATCAACAAAGTTGTTCAGCTCCAGGAAGAGCTTGACATCGATGTTTTGGTGCATGGAGAGCCTGAGGTAAGCATTTGTCTTCAAACAATGTTGAAGAAATTTAACTAACCATACATTGTCTAAGTTTAAACTGTAGCATCTGAATGTTAACGTCTCAGAGAAATGATATGGTTGAGTACTTTGGAGAGCAACTGTCTGGCTTTGCCTTCACTGTCAATGGTTGGGTCCAATCCTATGGGTCCCGTTGTGTCAAGCCGCCTATCATCTATGGTGATGTCAGTCGTCCAAAACCCATGACCGTCTTTTGGTCGACAATGGCTCAGAGCATGACGAAGCGGCCTATGAAGGGAATGCTTACTGGTCCAGTTACAATTTTGAACTGGTCTTTTGTTCGAAATGACCAACCAAGGTATGCGAATTTAATGAATCTCAGTTGCTGTTTCTTTGATCGTCTGTTTTATTGAGGGCAGTTCATGGTAGTTAATTCAATTGTTTTTCATAGGTTTGAAACATGCTACCAAATTGCCTTGGCAATCAAGGACGAGGTTGAGGATCTTGAGAAAGCTGGTATTACAGTCATCCAGATTGATGAAGCAGCACTGCGAGAAGGTTTGCCTCTCCGAAAGTCCGAGGAAAATTTCTATTTGAATTGGGCTGTTCATTCATTTCGAATCACCAATTGCGGTGTTCAAGATACCACCCAGGTCCTTTCCTACTCTCTTTTGCTTAAAAGTCCGATTATTTCTCTCACTAAATCAATTTGTTAGCGTTGTTTCATGTCATTTGCATTGAGTCGTATAACCGTCTTTTCAATGACATAGTGAACTTTATGATTTTGATGACTGCAGATCCACACCCACATGTGCTACTCGAACTTCAACGACATCATCCATTCGATAATCGACATGGATGCTGATGTGATCACCATTGAGAATTCCAGATCAGATGAGAAGCTCCTGTCTGTCTTCCGTGAGGGAGTAAAATACGGTGCTGGAATTGGACCAGGAGTCTACGACATCCATTCTCCCCGTATTCCCTCTACTGAGGAAATTGCTGACAGGATCAACAAGATGCTCGCTGTTCTTGAAAGCAACATCCTCTGGGTGAACCCTGACTGCGGTCTTAAAACCCGAAAGTACACTGAAGTCAAGCCTGCCCTCCTCAACATGGTTGCTGCTGCTAAGCTCCTTCGCTCCCAATTGGCCAGTGCCAAGTGAGTTTGAGAATGCTGTGGCAAACCTTAGGAAGAAAAACACTTAAAATTTTTCAATAAGATTTTGGCCTTCTGGTGAAGTGATCTTGTTGTTCTCTTTCTTGGAGTGTCTTCAATTTCTTAGGGAAGTAATGAACAATGTCGTCAAATTCCCATTGTGGCTTTTGTTTAAAAAGTTCAAAATTTTCTGTTTTTTTAATCAGTTTTTCTCGTTCAAGCCATTTTTTTTTGGTTTGTTTTGTAAAATAGTGGAGATGAGAATTAATGATATTAATAGATATACTTGTGTTTTGTCACACTACTTTGGGATAAAAGTAAAAGCAACAATTTAAAAGTATATTTTGTTTCCTGCATAATGATTCAGTTTCTATACTAAATTGTTACCGATTTAAGTTTACAGAGTTAAAAGTTTTGGGATCA

Coding sequence (CDS)

ATGGCATCCCACATTGTTGGTTATCCTCGCATTGGTCCGAAGAGAGAACTTAAATTTGCCTTGGAGTCTTTCTGGGATGGGAAGAGCAGTGCTGATGACTTGCAGAAAGTGGCTGCTGATCTCAGGTCATCTATTTGGAAGCAGATGGCTGAAGCTGGAATCAAGTTTATTCCAAGCAACACTTTTTCGTATTATGATCAAGTGCTTGACACTGCAACTATGCTCGGAGCTGTTCCGTCAAGATATGGATGGAACGGTGGTGAGATTGGATTTGACATCTACTTCTCCATGGCTAGAGGGAACGCTTCAGTTCCTGCCATGGAAATGACGAAGTGGTTCGATACCAATTACCATTACATTGTGCCCGAGCTGAGTCCAGAAGATAAATTTACTTATGCATCTCACAAAGCAGTAAACGAGTTCAAGGAAGCTAAAGCTCTCGGAGTTGAGACTGTCCCGGTTCTTGTAGGCCCTGTATCCTTCTTGTTGCTTTCAAAACCAGCCAAGGGAGTTGAGAAATCCTTCTCACTTCTCTCCCTAATCGACAAGATTCTTCCTGTTTATAAGGAAGTTGTTTCAGAGCTGAAGGCTGCTGGTGCCACTTGGATTCAATTTGATGAGCCTACACTTGTGAAAGATCTTGACGCGCACCACTTACAAGCATTTGCTCAAGCCTACTCAGAGCTGGAATCCACTTTATCTGGTTTAAATGTTCTGATTGAGACATATTTTGCTGATGTTCCTGCTGAGGCATACAAAACTCTCACGTCTTTGAAGGGTGTCTCTGGTTATGGATTCGATCTAGTTCGTGGAACACAGACACTTGATCTGATCAAAGGTGGATTTCCTTCAGGCAAATATCTCTTTGCCGGAGTGGTTGATGGAAGAAACATATGGTCAAATGACCTTCAAGCTTCCATAACTACCTTGGAGGCGTTGGAAAACGTTGTTGGAAAAGAAAAAATTGTCGTCTCCACTTCCTGCTCTCTTCTTCACACTGCTGTTGATCTGGTGAACGAAACAAAACTAGATGATGAAATTAAGTCCTGGCTTGCTTTTGCCGCTCAGAAAGTTGTTGAAGTTAATGCCTTGGCTAAGGCACTCTCTGGACATAAAGATGAGGCATATTTTTCTTCCAATGCTGCTGCTCAAGCTTCAAGGAAATCATCCCCAAGAGTGACAAATGAGGCAGTCCAAAAAGCTGCTGCTGCTCTGAAGGGCTCTGACCACCGTCGGTCAACAAATGTAAGTGCCAGGCTGGACGCTCAGCAGAAGAAGTTGAACCTTCCAATGCTGCCTACAACAACAATTGGATCCTTCCCTCAAACTATGGATCTTAGAAGAGTGCGACGTGAATTCAAAGCAAAAAAAATCTCTGAGGAAGACTATGTCAGCTCCATCAAGGAGGAAATCAACAAAGTTGTTCAGCTCCAGGAAGAGCTTGACATCGATGTTTTGGTGCATGGAGAGCCTGAGAGAAATGATATGGTTGAGTACTTTGGAGAGCAACTGTCTGGCTTTGCCTTCACTGTCAATGGTTGGGTCCAATCCTATGGGTCCCGTTGTGTCAAGCCGCCTATCATCTATGGTGATGTCAGTCGTCCAAAACCCATGACCGTCTTTTGGTCGACAATGGCTCAGAGCATGACGAAGCGGCCTATGAAGGGAATGCTTACTGGTCCAGTTACAATTTTGAACTGGTCTTTTGTTCGAAATGACCAACCAAGGTTTGAAACATGCTACCAAATTGCCTTGGCAATCAAGGACGAGGTTGAGGATCTTGAGAAAGCTGGTATTACAGTCATCCAGATTGATGAAGCAGCACTGCGAGAAGGTTTGCCTCTCCGAAAGTCCGAGGAAAATTTCTATTTGAATTGGGCTGTTCATTCATTTCGAATCACCAATTGCGGTGTTCAAGATACCACCCAGATCCACACCCACATGTGCTACTCGAACTTCAACGACATCATCCATTCGATAATCGACATGGATGCTGATGTGATCACCATTGAGAATTCCAGATCAGATGAGAAGCTCCTGTCTGTCTTCCGTGAGGGAGTAAAATACGGTGCTGGAATTGGACCAGGAGTCTACGACATCCATTCTCCCCGTATTCCCTCTACTGAGGAAATTGCTGACAGGATCAACAAGATGCTCGCTGTTCTTGAAAGCAACATCCTCTGGGTGAACCCTGACTGCGGTCTTAAAACCCGAAAGTACACTGAAGTCAAGCCTGCCCTCCTCAACATGGTTGCTGCTGCTAAGCTCCTTCGCTCCCAATTGGCCAGTGCCAAGTGA

Protein sequence

MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDGRNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK
Homology
BLAST of Cucsat.G7170 vs. ExPASy Swiss-Prot
Match: Q42699 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Catharanthus roseus OX=4058 GN=METE PE=2 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 1.0e-243
Identity = 422/492 (85.77%), Postives = 467/492 (94.92%), Query Frame = 0

Query: 66  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 125
           MASHIVGYPR+GPKRELKFALESFWD KSSA+DLQKVAADLRSSIWKQMA+AGIK+IPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDKKSSAEDLQKVAADLRSSIWKQMADAGIKYIPSN 60

Query: 126 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 185
           TFSYYDQVLDTATMLGAVP RY + GGEIGFD YFSMARGNASVPAMEMTKWFDTNYHYI
Sbjct: 61  TFSYYDQVLDTATMLGAVPPRYNFAGGEIGFDTYFSMARGNASVPAMEMTKWFDTNYHYI 120

Query: 186 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 245
           VPEL PE  F+YASHKAVNE+KEAK LGV+TVPVLVGPV+FLLLSKPAKGVEK+F LLSL
Sbjct: 121 VPELGPEVNFSYASHKAVNEYKEAKELGVDTVPVLVGPVTFLLLSKPAKGVEKTFPLLSL 180

Query: 246 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLNVLI 305
           +DKILPVYKEV+ ELKAAGA+WIQFDEPTLV DL++H L+AF +AYSELESTLSGLNV++
Sbjct: 181 LDKILPVYKEVIGELKAAGASWIQFDEPTLVLDLESHQLEAFTKAYSELESTLSGLNVIV 240

Query: 306 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDGRNIWS 365
           ETYFAD+PAE YK LT+LKGV+G+GFDLVRG +TLDLIKGGFPSGKYLFAGVVDGRNIW+
Sbjct: 241 ETYFADIPAETYKILTALKGVTGFGFDLVRGAKTLDLIKGGFPSGKYLFAGVVDGRNIWA 300

Query: 366 NDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVVE 425
           NDL AS++TL++LE +VGK+K+VVSTSCSLLHTAVDLVNE KLD EIKSWLAFAAQKVVE
Sbjct: 301 NDLAASLSTLQSLEGIVGKDKLVVSTSCSLLHTAVDLVNEPKLDKEIKSWLAFAAQKVVE 360

Query: 426 VNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSARL 485
           VNALAKAL+G KDEA+FS NAAAQASRKSSPRVTN+AVQKAAAAL+GSDHRR+T VSARL
Sbjct: 361 VNALAKALAGEKDEAFFSENAAAQASRKSSPRVTNQAVQKAAAALRGSDHRRATTVSARL 420

Query: 486 DAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQEE 545
           DAQQKKLNLP+LPTTTIGSFPQT++LRRVRRE+KAKKISE+DYV +IKEEI+KVV+LQEE
Sbjct: 421 DAQQKKLNLPVLPTTTIGSFPQTLELRRVRREYKAKKISEDDYVKAIKEEISKVVKLQEE 480

Query: 546 LDIDVLVHGEPE 558
           LDIDVLVHGEPE
Sbjct: 481 LDIDVLVHGEPE 492

BLAST of Cucsat.G7170 vs. ExPASy Swiss-Prot
Match: Q2QLY5 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0623900 PE=2 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 2.8e-241
Identity = 417/493 (84.58%), Postives = 465/493 (94.32%), Query Frame = 0

Query: 66  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 125
           MASHIVGYPR+GPKRELKFALESFWDGKSSA+DL+KVA DLR+SIWKQMA+AGIK+IPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDGKSSAEDLEKVATDLRASIWKQMADAGIKYIPSN 60

Query: 126 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 185
           TFSYYDQVLDTA MLGAVP RY W GGEIGF  YFSMARGNA+VPAMEMTKWFDTNYH+I
Sbjct: 61  TFSYYDQVLDTAAMLGAVPERYSWTGGEIGFSTYFSMARGNATVPAMEMTKWFDTNYHFI 120

Query: 186 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 245
           VPEL P  KF+Y+SHKAVNE+KEAKALGV+TVPVLVGPVS+LLLSKPAKGVEKSF+LLSL
Sbjct: 121 VPELGPNTKFSYSSHKAVNEYKEAKALGVDTVPVLVGPVSYLLLSKPAKGVEKSFALLSL 180

Query: 246 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLNVLI 305
           +  ILPVYKEV++ELKAAGATWIQFDEPTLV DLD+H L AF+ AY+ELES LSGLNVLI
Sbjct: 181 LSSILPVYKEVIAELKAAGATWIQFDEPTLVLDLDSHQLAAFSAAYTELESALSGLNVLI 240

Query: 306 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIK-GGFPSGKYLFAGVVDGRNIW 365
           ETYFAD+PAE+YKTLTSL  V+ YGFDL+RG +TLDLIK  GFPSGKYLFAGVVDGRNIW
Sbjct: 241 ETYFADIPAESYKTLTSLNSVTAYGFDLIRGAKTLDLIKSAGFPSGKYLFAGVVDGRNIW 300

Query: 366 SNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVV 425
           ++DL AS+TTLE+LE +VGK+K+VVSTSCSL+HTAVDLVNETKLD EIKSWLAFAAQKVV
Sbjct: 301 ADDLAASLTTLESLEAIVGKDKLVVSTSCSLMHTAVDLVNETKLDSEIKSWLAFAAQKVV 360

Query: 426 EVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSAR 485
           EVNALAKAL+G KDEAYF++NAAAQASR+SSPRVTNE VQKAAAALKGSDHRR+TNVSAR
Sbjct: 361 EVNALAKALAGQKDEAYFAANAAAQASRRSSPRVTNEEVQKAAAALKGSDHRRATNVSAR 420

Query: 486 LDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQE 545
           LDAQQKKLNLP+LPTTTIGSFPQT++LRRVRRE+KAKKISEE+Y+S+IKEEI+KVV++QE
Sbjct: 421 LDAQQKKLNLPVLPTTTIGSFPQTVELRRVRREYKAKKISEEEYISAIKEEISKVVKIQE 480

Query: 546 ELDIDVLVHGEPE 558
           ELDIDVLVHGEPE
Sbjct: 481 ELDIDVLVHGEPE 493

BLAST of Cucsat.G7170 vs. ExPASy Swiss-Prot
Match: Q2QLY4 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0624000 PE=2 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 8.9e-240
Identity = 413/493 (83.77%), Postives = 464/493 (94.12%), Query Frame = 0

Query: 66  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 125
           MASHIVGYPR+GPKRELKFALESFWDGKSSA+DL+KVA DLR+SIWKQMA+AGIK+IPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDGKSSAEDLEKVATDLRASIWKQMADAGIKYIPSN 60

Query: 126 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 185
           TFSYYDQVLDT  MLGAVP RY W GGEIGF  YFSMARGNA+VPAMEMTKWFDTNYH+I
Sbjct: 61  TFSYYDQVLDTTAMLGAVPERYSWTGGEIGFSTYFSMARGNATVPAMEMTKWFDTNYHFI 120

Query: 186 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 245
           VPEL P  KF+Y+SHKAVNE+KEAKALGV+TVPVLVGPVS+LLLSKPAKGVEKSF+LLSL
Sbjct: 121 VPELGPNTKFSYSSHKAVNEYKEAKALGVDTVPVLVGPVSYLLLSKPAKGVEKSFALLSL 180

Query: 246 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLNVLI 305
           +  ILPVYKEV++ELKAAGATWIQFDEPTLV DLD+H L AF+ AY+ELES LSGLNVLI
Sbjct: 181 LSSILPVYKEVIAELKAAGATWIQFDEPTLVLDLDSHQLAAFSAAYTELESALSGLNVLI 240

Query: 306 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIK-GGFPSGKYLFAGVVDGRNIW 365
           ETYFAD+PAE+YKTLTSL  V+ YGFDL+RG++TLDL+K  GFPSGKYLFAGVVDGRNIW
Sbjct: 241 ETYFADIPAESYKTLTSLNSVTAYGFDLIRGSKTLDLVKSAGFPSGKYLFAGVVDGRNIW 300

Query: 366 SNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVV 425
           ++DL AS+TTLE+LE +VGK+K+VVSTSCSL+HTAVDLVNETKLD EIKSWLAFAAQKVV
Sbjct: 301 ADDLAASLTTLESLEAIVGKDKLVVSTSCSLMHTAVDLVNETKLDSEIKSWLAFAAQKVV 360

Query: 426 EVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSAR 485
           EVNALAKAL+G KDEAYF++N AAQASR+SSPRVTNE VQKAAAAL+GSDHRR+TNVSAR
Sbjct: 361 EVNALAKALAGQKDEAYFAANTAAQASRRSSPRVTNEEVQKAAAALRGSDHRRATNVSAR 420

Query: 486 LDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQE 545
           LDAQQKKLNLP+LPTTTIGSFPQT++LRRVRRE+KAKKISE++YVS+IKEEI+KVV++QE
Sbjct: 421 LDAQQKKLNLPVLPTTTIGSFPQTVELRRVRREYKAKKISEDEYVSAIKEEISKVVKIQE 480

Query: 546 ELDIDVLVHGEPE 558
           ELDIDVLVHGEPE
Sbjct: 481 ELDIDVLVHGEPE 493

BLAST of Cucsat.G7170 vs. ExPASy Swiss-Prot
Match: P93263 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Mesembryanthemum crystallinum OX=3544 GN=METE PE=2 SV=1)

HSP 1 Score: 830.5 bits (2144), Expect = 1.2e-239
Identity = 416/492 (84.55%), Postives = 462/492 (93.90%), Query Frame = 0

Query: 66  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 125
           MASHIVGYPR+GPKRELKFALESFWDGKS+A+DL+KV+ADLRSSIWKQMA+AGIK+IPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDGKSTAEDLKKVSADLRSSIWKQMADAGIKYIPSN 60

Query: 126 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 185
           TFSYYDQVLDT  MLGAVP RYGW GGEI FD+YFSMARGNASVPAMEMTKWFDTNYH+I
Sbjct: 61  TFSYYDQVLDTTAMLGAVPPRYGWTGGEIEFDVYFSMARGNASVPAMEMTKWFDTNYHFI 120

Query: 186 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 245
           VPEL PE  F+YASHKAV E+KEAKALGV+TVPVLVGPVS+LLLSK AKGV+KSF LLSL
Sbjct: 121 VPELGPEVNFSYASHKAVLEYKEAKALGVDTVPVLVGPVSYLLLSKQAKGVDKSFDLLSL 180

Query: 246 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLNVLI 305
           + KILP+YKEVV+ELK AGA+WIQFDEP LV DL++H LQAF+ AY++LESTLSGLNV++
Sbjct: 181 LPKILPIYKEVVAELKEAGASWIQFDEPLLVMDLESHKLQAFSAAYADLESTLSGLNVVV 240

Query: 306 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDGRNIWS 365
           ETYFADV AEAYKTL SLKGV+GYGFDLVRGT+TLDL+K  FPSGKYLFAGVVDGRNIW+
Sbjct: 241 ETYFADVTAEAYKTLISLKGVTGYGFDLVRGTKTLDLVKAEFPSGKYLFAGVVDGRNIWA 300

Query: 366 NDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVVE 425
           NDL AS+ TLEALE VVGK+K+VVSTSCS LHTAVDL+NETKLDDEIKSWLAFAAQKV+E
Sbjct: 301 NDLAASLATLEALEGVVGKDKLVVSTSCSFLHTAVDLINETKLDDEIKSWLAFAAQKVLE 360

Query: 426 VNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSARL 485
           VNALAKALSG KDEA+FS+NAAA ASRKSSPRVTNEAVQKAA ALKGSDHRR+T VS+RL
Sbjct: 361 VNALAKALSGQKDEAFFSANAAALASRKSSPRVTNEAVQKAATALKGSDHRRATTVSSRL 420

Query: 486 DAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQEE 545
           DAQQKKLNLP+LPTTTIGSFPQT++LRRVRRE+KAKKISEE+YV +IKEEI+KVV+LQEE
Sbjct: 421 DAQQKKLNLPILPTTTIGSFPQTVELRRVRREYKAKKISEEEYVKAIKEEISKVVKLQEE 480

Query: 546 LDIDVLVHGEPE 558
           LDIDVLVHGEPE
Sbjct: 481 LDIDVLVHGEPE 492

BLAST of Cucsat.G7170 vs. ExPASy Swiss-Prot
Match: O50008 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=MS1 PE=1 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 1.5e-239
Identity = 414/492 (84.15%), Postives = 458/492 (93.09%), Query Frame = 0

Query: 66  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 125
           MASHIVGYPR+GPKRELKFALESFWDGKS+A+DLQKV+ADLRSSIWKQM+ AG KFIPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDGKSTAEDLQKVSADLRSSIWKQMSAAGTKFIPSN 60

Query: 126 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 185
           TF++YDQVLDT  MLGAVP RYG+ GGEIG D+YFSMARGNASVPAMEMTKWFDTNYHYI
Sbjct: 61  TFAHYDQVLDTTAMLGAVPPRYGYTGGEIGLDVYFSMARGNASVPAMEMTKWFDTNYHYI 120

Query: 186 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 245
           VPEL PE  F+YASHKAVNE+KEAKALGV+TVPVLVGPVS+LLLSK AKGV+KSF LLSL
Sbjct: 121 VPELGPEVNFSYASHKAVNEYKEAKALGVDTVPVLVGPVSYLLLSKAAKGVDKSFELLSL 180

Query: 246 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLNVLI 305
           + KILP+YKEV++ELKAAGATWIQ DEP LV DL+   LQAF  AY+ELESTLSGLNVL+
Sbjct: 181 LPKILPIYKEVITELKAAGATWIQLDEPVLVMDLEGQKLQAFTGAYAELESTLSGLNVLV 240

Query: 306 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDGRNIWS 365
           ETYFAD+PAEAYKTLTSLKGV+ +GFDLVRGT+TLDL+K GFP GKYLFAGVVDGRNIW+
Sbjct: 241 ETYFADIPAEAYKTLTSLKGVTAFGFDLVRGTKTLDLVKAGFPEGKYLFAGVVDGRNIWA 300

Query: 366 NDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVVE 425
           ND  AS++TL+ALE +VGK+K+VVSTSCSLLHTAVDL+NETKLDDEIKSWLAFAAQKVVE
Sbjct: 301 NDFAASLSTLQALEGIVGKDKLVVSTSCSLLHTAVDLINETKLDDEIKSWLAFAAQKVVE 360

Query: 426 VNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSARL 485
           VNALAKAL+G KDEA FS+NAAA ASR+SSPRVTNE VQKAAAALKGSDHRR+TNVSARL
Sbjct: 361 VNALAKALAGQKDEALFSANAAALASRRSSPRVTNEGVQKAAAALKGSDHRRATNVSARL 420

Query: 486 DAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQEE 545
           DAQQKKLNLP+LPTTTIGSFPQT++LRRVRRE+KAKK+SEEDYV +IKEEI KVV LQEE
Sbjct: 421 DAQQKKLNLPILPTTTIGSFPQTVELRRVRREYKAKKVSEEDYVKAIKEEIKKVVDLQEE 480

Query: 546 LDIDVLVHGEPE 558
           LDIDVLVHGEPE
Sbjct: 481 LDIDVLVHGEPE 492

BLAST of Cucsat.G7170 vs. NCBI nr
Match: XP_004144074.1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [Cucumis sativus] >KGN66367.1 hypothetical protein Csa_007688 [Cucumis sativus])

HSP 1 Score: 1048 bits (2709), Expect = 0.0
Identity = 551/557 (98.92%), Postives = 553/557 (99.28%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MTQLPLKSLEPFAISSSSSSS   FSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIR+TS
Sbjct: 1   MTQLPLKSLEPFAISSSSSSS---FSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRSTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK
Sbjct: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAF QAYSELESTLSG
Sbjct: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQT+DLIKGGFPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480
           QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480

Query: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540
           VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV
Sbjct: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540

Query: 541 QLQEELDIDVLVHGEPE 557
           QLQEELDIDVLVHGEPE
Sbjct: 541 QLQEELDIDVLVHGEPE 554

BLAST of Cucsat.G7170 vs. NCBI nr
Match: XP_008451024.1 (PREDICTED: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [Cucumis melo])

HSP 1 Score: 1042 bits (2695), Expect = 0.0
Identity = 550/557 (98.74%), Postives = 553/557 (99.28%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MTQLPLKSLEPFAISSSSSSSSSS SIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS
Sbjct: 1   MTQLPLKSLEPFAISSSSSSSSSS-SIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMA+AGIK
Sbjct: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMADAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELESTLSG
Sbjct: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESTLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSN+LQASITTLEAL NVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNNLQASITTLEALGNVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480
           QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRR+TN
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATN 480

Query: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540
           VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV
Sbjct: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540

Query: 541 QLQEELDIDVLVHGEPE 557
           QLQEELDIDVLVHGEPE
Sbjct: 541 QLQEELDIDVLVHGEPE 556

BLAST of Cucsat.G7170 vs. NCBI nr
Match: XP_038879499.1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 1033 bits (2670), Expect = 0.0
Identity = 542/557 (97.31%), Postives = 549/557 (98.56%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MTQLPLKSLEPFAISSSSSSSSS   IFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS
Sbjct: 1   MTQLPLKSLEPFAISSSSSSSSS---IFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMA+AGIK
Sbjct: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMADAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNE+KEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEYKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSL+DKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELES LSG
Sbjct: 241 SLLSLVDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESVLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSNDLQASITTLEALE VVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNDLQASITTLEALEKVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480
           QKVVEVNALAKAL+GHKDEAYFS+NAAAQASRKSSPRVTNEAVQKAAAALKGSDHRR+TN
Sbjct: 421 QKVVEVNALAKALAGHKDEAYFSANAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATN 480

Query: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540
           VS RLDAQQKKLNLP+LPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV
Sbjct: 481 VSVRLDAQQKKLNLPILPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540

Query: 541 QLQEELDIDVLVHGEPE 557
           QLQEELDIDVLVHGEPE
Sbjct: 541 QLQEELDIDVLVHGEPE 554

BLAST of Cucsat.G7170 vs. NCBI nr
Match: XP_023533330.1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 987 bits (2551), Expect = 0.0
Identity = 517/557 (92.82%), Postives = 532/557 (95.51%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MT LPLKSL+ F ISS SSSS+S     ALRRG IAPFSYLL SSIH LPSLRLSIRTTS
Sbjct: 1   MTLLPLKSLDSFGISSCSSSSTS----IALRRGTIAPFSYLLVSSIHRLPSLRLSIRTTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASH+VGYPRIGPKRELKFALESFWDGKS+ADDLQKVA DLR+SIWKQMA+AGIK
Sbjct: 61  TVVRAMASHVVGYPRIGPKRELKFALESFWDGKSTADDLQKVATDLRASIWKQMADAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFD YFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDTYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNE+KEAKALGVET+PVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEYKEAKALGVETIPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSL+DKILP+YKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELES LSG
Sbjct: 241 SLLSLVDKILPIYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESVLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKG FPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGEFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSNDLQAS+ TLEAL+NVVGKE IVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNDLQASLNTLEALQNVVGKENIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480
           QKVVEVNALAKALSGHKDEAYFS+NAAAQASRKSSPRVTNEAVQKAAAALK SDHRR+TN
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSANAAAQASRKSSPRVTNEAVQKAAAALKSSDHRRATN 480

Query: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540
           VS RLDAQQKKLNLP LPTTTIGSFPQTMDLRRVRREFKAKKISEE YVS+IKEEINKVV
Sbjct: 481 VSVRLDAQQKKLNLPKLPTTTIGSFPQTMDLRRVRREFKAKKISEEVYVSNIKEEINKVV 540

Query: 541 QLQEELDIDVLVHGEPE 557
           QLQEEL IDVLVHGEPE
Sbjct: 541 QLQEELGIDVLVHGEPE 553

BLAST of Cucsat.G7170 vs. NCBI nr
Match: XP_022147614.1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like [Momordica charantia])

HSP 1 Score: 987 bits (2551), Expect = 0.0
Identity = 516/555 (92.97%), Postives = 531/555 (95.68%), Query Frame = 0

Query: 3   QLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTSTV 62
           QL LKS EPFA SS+SS       I ALRRGAI+PF YLLFSSIHHLPS RLSIRTTSTV
Sbjct: 5   QLSLKSHEPFAKSSASS-------IIALRRGAISPFPYLLFSSIHHLPSRRLSIRTTSTV 64

Query: 63  VRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFI 122
           +RAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMA+AGIKFI
Sbjct: 65  IRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMADAGIKFI 124

Query: 123 PSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNY 182
           PSNTFSYYDQVLDTATMLGAVP RYGWNGGEIGFD YFSMARGNASVPAMEMTKWFDTNY
Sbjct: 125 PSNTFSYYDQVLDTATMLGAVPPRYGWNGGEIGFDTYFSMARGNASVPAMEMTKWFDTNY 184

Query: 183 HYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSL 242
           HYIVPELSP+DKF YASHKAVNE+KEAKALGV+T+PVLVGPVSFL+LSKPAKGVEKSFSL
Sbjct: 185 HYIVPELSPDDKFIYASHKAVNEYKEAKALGVDTIPVLVGPVSFLVLSKPAKGVEKSFSL 244

Query: 243 LSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLN 302
           LSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELES LSGLN
Sbjct: 245 LSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESVLSGLN 304

Query: 303 VLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDGRN 362
           VLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQT DLIK GFPSGKYLFAGVVDGRN
Sbjct: 305 VLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTFDLIKSGFPSGKYLFAGVVDGRN 364

Query: 363 IWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQK 422
           IWSNDLQAS+ TL+ALEN VGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQK
Sbjct: 365 IWSNDLQASLNTLQALENAVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQK 424

Query: 423 VVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVS 482
           V+EVNALAKALSG KDEAYFS+NAAAQASRKSSPRVTNEAVQKAAAALKGSDHRR+TNVS
Sbjct: 425 VIEVNALAKALSGKKDEAYFSTNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATNVS 484

Query: 483 ARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQL 542
           ARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEE+YVSSIKEEINKVVQL
Sbjct: 485 ARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEENYVSSIKEEINKVVQL 544

Query: 543 QEELDIDVLVHGEPE 557
           QEELDIDVLVHGEPE
Sbjct: 545 QEELDIDVLVHGEPE 552

BLAST of Cucsat.G7170 vs. ExPASy TrEMBL
Match: A0A0A0LZR2 (5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_1G599580 PE=3 SV=1)

HSP 1 Score: 1048 bits (2709), Expect = 0.0
Identity = 551/557 (98.92%), Postives = 553/557 (99.28%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MTQLPLKSLEPFAISSSSSSS   FSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIR+TS
Sbjct: 1   MTQLPLKSLEPFAISSSSSSS---FSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRSTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK
Sbjct: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAF QAYSELESTLSG
Sbjct: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQT+DLIKGGFPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480
           QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480

Query: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540
           VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV
Sbjct: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540

Query: 541 QLQEELDIDVLVHGEPE 557
           QLQEELDIDVLVHGEPE
Sbjct: 541 QLQEELDIDVLVHGEPE 554

BLAST of Cucsat.G7170 vs. ExPASy TrEMBL
Match: A0A1S3BQJ8 (5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103492431 PE=3 SV=1)

HSP 1 Score: 1042 bits (2695), Expect = 0.0
Identity = 550/557 (98.74%), Postives = 553/557 (99.28%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MTQLPLKSLEPFAISSSSSSSSSS SIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS
Sbjct: 1   MTQLPLKSLEPFAISSSSSSSSSS-SIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMA+AGIK
Sbjct: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMADAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELESTLSG
Sbjct: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESTLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSN+LQASITTLEAL NVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNNLQASITTLEALGNVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480
           QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRR+TN
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATN 480

Query: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540
           VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV
Sbjct: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540

Query: 541 QLQEELDIDVLVHGEPE 557
           QLQEELDIDVLVHGEPE
Sbjct: 541 QLQEELDIDVLVHGEPE 556

BLAST of Cucsat.G7170 vs. ExPASy TrEMBL
Match: A0A6J1D1J0 (5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111016496 PE=3 SV=1)

HSP 1 Score: 987 bits (2551), Expect = 0.0
Identity = 516/555 (92.97%), Postives = 531/555 (95.68%), Query Frame = 0

Query: 3   QLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTSTV 62
           QL LKS EPFA SS+SS       I ALRRGAI+PF YLLFSSIHHLPS RLSIRTTSTV
Sbjct: 5   QLSLKSHEPFAKSSASS-------IIALRRGAISPFPYLLFSSIHHLPSRRLSIRTTSTV 64

Query: 63  VRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFI 122
           +RAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMA+AGIKFI
Sbjct: 65  IRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMADAGIKFI 124

Query: 123 PSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNY 182
           PSNTFSYYDQVLDTATMLGAVP RYGWNGGEIGFD YFSMARGNASVPAMEMTKWFDTNY
Sbjct: 125 PSNTFSYYDQVLDTATMLGAVPPRYGWNGGEIGFDTYFSMARGNASVPAMEMTKWFDTNY 184

Query: 183 HYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSL 242
           HYIVPELSP+DKF YASHKAVNE+KEAKALGV+T+PVLVGPVSFL+LSKPAKGVEKSFSL
Sbjct: 185 HYIVPELSPDDKFIYASHKAVNEYKEAKALGVDTIPVLVGPVSFLVLSKPAKGVEKSFSL 244

Query: 243 LSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLN 302
           LSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELES LSGLN
Sbjct: 245 LSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESVLSGLN 304

Query: 303 VLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDGRN 362
           VLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQT DLIK GFPSGKYLFAGVVDGRN
Sbjct: 305 VLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTFDLIKSGFPSGKYLFAGVVDGRN 364

Query: 363 IWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQK 422
           IWSNDLQAS+ TL+ALEN VGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQK
Sbjct: 365 IWSNDLQASLNTLQALENAVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQK 424

Query: 423 VVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVS 482
           V+EVNALAKALSG KDEAYFS+NAAAQASRKSSPRVTNEAVQKAAAALKGSDHRR+TNVS
Sbjct: 425 VIEVNALAKALSGKKDEAYFSTNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATNVS 484

Query: 483 ARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQL 542
           ARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEE+YVSSIKEEINKVVQL
Sbjct: 485 ARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEENYVSSIKEEINKVVQL 544

Query: 543 QEELDIDVLVHGEPE 557
           QEELDIDVLVHGEPE
Sbjct: 545 QEELDIDVLVHGEPE 552

BLAST of Cucsat.G7170 vs. ExPASy TrEMBL
Match: A0A6J1JBY9 (5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111485391 PE=3 SV=1)

HSP 1 Score: 979 bits (2531), Expect = 0.0
Identity = 513/557 (92.10%), Postives = 528/557 (94.79%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MT LP+KSL+ F IS  SSSS+S     ALRRG IAPFSYLL SSIH LPSLRLSIRT S
Sbjct: 1   MTLLPVKSLDSFGISPCSSSSTS----IALRRGTIAPFSYLLVSSIHRLPSLRLSIRTAS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASH+VGYPRIGPKRELKFALESFWDGKS+ADDLQKVA DLR+SIWKQMA+AGIK
Sbjct: 61  TVVRAMASHVVGYPRIGPKRELKFALESFWDGKSTADDLQKVATDLRASIWKQMADAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFD YFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDTYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNE+KEAKALGVET+PVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEYKEAKALGVETIPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSL+ KILP+YKEVVSELKAAGATWIQFDEPTLVKDLD H LQAFA AYSELES LSG
Sbjct: 241 SLLSLVGKILPIYKEVVSELKAAGATWIQFDEPTLVKDLDTHQLQAFAHAYSELESVLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKG FPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGKFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSNDLQAS+ TLEAL+NVVGKE IVVSTSCSLLHTAVDLVNETKLDDEIK WLAFAA
Sbjct: 361 RNIWSNDLQASLNTLEALQNVVGKENIVVSTSCSLLHTAVDLVNETKLDDEIKLWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480
           QKVVEVNALAKALSGHKDEAYFS+NAAAQASRKSSPRVTNEAVQKAAAALK SDHRR+TN
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSANAAAQASRKSSPRVTNEAVQKAAAALKSSDHRRATN 480

Query: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540
           VS RLDAQQKKLNLP LPTTTIGSFPQTMDLRRVRREFKAKKISEE YVSSIKEEINKVV
Sbjct: 481 VSVRLDAQQKKLNLPKLPTTTIGSFPQTMDLRRVRREFKAKKISEEVYVSSIKEEINKVV 540

Query: 541 QLQEELDIDVLVHGEPE 557
           QLQEELDIDVLVHGEPE
Sbjct: 541 QLQEELDIDVLVHGEPE 553

BLAST of Cucsat.G7170 vs. ExPASy TrEMBL
Match: A0A6J1HAA9 (5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111461556 PE=3 SV=1)

HSP 1 Score: 979 bits (2531), Expect = 0.0
Identity = 513/557 (92.10%), Postives = 528/557 (94.79%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MT LPLKSL+ F ISS SSSS+S     ALRRG I PFSYLL SSIH  PSLRLSIRTTS
Sbjct: 1   MTLLPLKSLDSFGISSCSSSSTS----IALRRGTITPFSYLLVSSIHRRPSLRLSIRTTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASH+VGYPRIGPKRELKFALESFWDGKS+ADDLQKVA DLR+SIWKQMA+AGIK
Sbjct: 61  TVVRAMASHVVGYPRIGPKRELKFALESFWDGKSTADDLQKVATDLRASIWKQMADAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFD YFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDTYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNE+KEAKALGVET+PVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEYKEAKALGVETIPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSL+DKILP+YKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELES LSG
Sbjct: 241 SLLSLVDKILPIYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESVLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKG FPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGEFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSNDLQAS+ TLEAL+NVVGKE IVVSTSCSLLHTAVDL NETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNDLQASLNTLEALQNVVGKENIVVSTSCSLLHTAVDLANETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480
           QKVVEVNALAKALSGHKDEAYFS+NAAAQASRKSSPRVTNEAVQKAAAALK SDHRR+TN
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSANAAAQASRKSSPRVTNEAVQKAAAALKSSDHRRATN 480

Query: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540
           VS RLDAQQKKL LP LPTTTIGSFPQTMDLRRVRREFKAKKISEE YVS+IKEEINKVV
Sbjct: 481 VSVRLDAQQKKLKLPKLPTTTIGSFPQTMDLRRVRREFKAKKISEEVYVSNIKEEINKVV 540

Query: 541 QLQEELDIDVLVHGEPE 557
           QLQEEL IDVLVHGEPE
Sbjct: 541 QLQEELGIDVLVHGEPE 553

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q426991.0e-24385.775-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Cathara... [more]
Q2QLY52.8e-24184.585-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 OS=Oryza... [more]
Q2QLY48.9e-24083.775-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2 OS=Oryza... [more]
P932631.2e-23984.555-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Mesembr... [more]
O500081.5e-23984.155-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 OS=Arabi... [more]
Match NameE-valueIdentityDescription
XP_004144074.10.098.925-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [Cucumis s... [more]
XP_008451024.10.098.74PREDICTED: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase... [more]
XP_038879499.10.097.315-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1-like [Be... [more]
XP_023533330.10.092.825-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1-like [Cu... [more]
XP_022147614.10.092.975-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like [Momo... [more]
Match NameE-valueIdentityDescription
A0A0A0LZR20.098.925-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucum... [more]
A0A1S3BQJ80.098.745-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucum... [more]
A0A6J1D1J00.092.975-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Momor... [more]
A0A6J1JBY90.092.105-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucur... [more]
A0A6J1HAA90.092.105-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucur... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013215Cobalamin-independent methionine synthase MetE, N-terminalPFAMPF08267Meth_synt_1coord: 68..381
e-value: 2.5E-120
score: 401.7
IPR038071UROD/MetE-like superfamilyGENE3D3.20.20.210coord: 70..590
e-value: 0.0
score: 1157.5
IPR038071UROD/MetE-like superfamilyGENE3D3.20.20.210coord: 455..823
e-value: 0.0
score: 1157.5
IPR038071UROD/MetE-like superfamilySUPERFAMILY51726UROD/MetE-likecoord: 68..460
IPR038071UROD/MetE-like superfamilySUPERFAMILY51726UROD/MetE-likecoord: 462..824
IPR006276Cobalamin-independent methionine synthaseTIGRFAMTIGR01371TIGR01371coord: 71..825
e-value: 0.0
score: 1152.2
IPR006276Cobalamin-independent methionine synthaseHAMAPMF_00172Meth_synthcoord: 66..825
score: 34.788776
IPR002629Cobalamin-independent methionine synthase MetE, C-terminal/archaealPFAMPF01717Meth_synt_2coord: 497..820
e-value: 4.1E-158
score: 525.8
IPR002629Cobalamin-independent methionine synthase MetE, C-terminal/archaealCDDcd03311CIMS_C_terminal_likecoord: 498..821
e-value: 1.64363E-147
score: 435.118
NoneNo IPR availablePANTHERPTHR30519:SF175-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE METHYLTRANSFERASE 3, CHLOROPLASTICcoord: 38..830
NoneNo IPR availablePANTHERPTHR305195-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE METHYLTRANSFERASEcoord: 38..830
NoneNo IPR availableCDDcd03312CIMS_N_terminal_likecoord: 68..432
e-value: 0.0
score: 599.903

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G7170.T27Cucsat.G7170.T27mRNA
Cucsat.G7170.T13Cucsat.G7170.T13mRNA
Cucsat.G7170.T2Cucsat.G7170.T2mRNA
Cucsat.G7170.T3Cucsat.G7170.T3mRNA
Cucsat.G7170.T1Cucsat.G7170.T1mRNA
Cucsat.G7170.T6Cucsat.G7170.T6mRNA
Cucsat.G7170.T7Cucsat.G7170.T7mRNA
Cucsat.G7170.T4Cucsat.G7170.T4mRNA
Cucsat.G7170.T5Cucsat.G7170.T5mRNA
Cucsat.G7170.T8Cucsat.G7170.T8mRNA
Cucsat.G7170.T9Cucsat.G7170.T9mRNA
Cucsat.G7170.T28Cucsat.G7170.T28mRNA
Cucsat.G7170.T25Cucsat.G7170.T25mRNA
Cucsat.G7170.T24Cucsat.G7170.T24mRNA
Cucsat.G7170.T26Cucsat.G7170.T26mRNA
Cucsat.G7170.T30Cucsat.G7170.T30mRNA
Cucsat.G7170.T29Cucsat.G7170.T29mRNA
Cucsat.G7170.T31Cucsat.G7170.T31mRNA
Cucsat.G7170.T14Cucsat.G7170.T14mRNA
Cucsat.G7170.T11Cucsat.G7170.T11mRNA
Cucsat.G7170.T18Cucsat.G7170.T18mRNA
Cucsat.G7170.T12Cucsat.G7170.T12mRNA
Cucsat.G7170.T19Cucsat.G7170.T19mRNA
Cucsat.G7170.T15Cucsat.G7170.T15mRNA
Cucsat.G7170.T10Cucsat.G7170.T10mRNA
Cucsat.G7170.T16Cucsat.G7170.T16mRNA
Cucsat.G7170.T21Cucsat.G7170.T21mRNA
Cucsat.G7170.T23Cucsat.G7170.T23mRNA
Cucsat.G7170.T17Cucsat.G7170.T17mRNA
Cucsat.G7170.T20Cucsat.G7170.T20mRNA
Cucsat.G7170.T22Cucsat.G7170.T22mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009086 methionine biosynthetic process
biological_process GO:0032259 methylation
biological_process GO:0008652 cellular amino acid biosynthetic process
cellular_component GO:0009507 chloroplast
cellular_component GO:0005829 cytosol
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003871 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity
molecular_function GO:0004045 aminoacyl-tRNA hydrolase activity
molecular_function GO:0008705 methionine synthase activity
molecular_function GO:0008270 zinc ion binding