Cucsat.G7170.T1 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G7170.T1
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
Locationctg1528: 2816297 .. 2829521 (-)
RNA-Seq ExpressionCucsat.G7170.T1
SyntenyCucsat.G7170.T1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAGCAAACAAACCTATACAATGCACATACAAAAACCCAAAACATACAGATCAACAATGTGTATTTTATAAGTAAAACAATTGTGTGGTGATTAATCGAACTCATCTAGTTGTGATATAATTGTTGACGAATGGTTTGAATCTAAAAAGGGCTATAGTAAATTCGTCCATACTCCTACCTCTAGGTTGGAAGGTCATGACATCGTTAGAGTACATTAAGAATTCGAAACCACAAGTAGAAGCACAAAAGCAAGTAAGGACTAAGGCGTCGGACTCAAACTTATATGAGTATTAAAATAGAATTCTCAAACTTAGTCTCAAACTTGTATAGCTCTTTGATTTCACCATTTTCGCGGCTTCTTCTTCAATTACACTCTCACACCAATTCTATCTTCCCTTCTCTTCGTCTTTGTTTTTTCAATTTCCCACTTAATTCAAGTTCCGGAATCATCTTCCCACCGGCCCCGATTGACGGTAACAACATTCCTTCTCTATCTCGTTGATCTTCAAGCTGTTTTTGTAATCTGGGTCATAATCAACTCAGGAACATAGTTTCCAATATTTTTTTTGGTGGCAACTCAACCACAACGCTGTTTATTACTTTTACATTTTGAATTTCTTCATCTTGTTATCGATTGATTAGCAGCTGATGATTTTTTTGTGTGTGTGTGTGTATGTTCTCAGTTTATAGGGAAGTGCATGAGCGGGTTTTTTTTAGTGGAAAATGTTCAATAAGTTAACCAAGCGTGGCTTTTGTACGGCAATATCATCACGACCTTGGCTTTTTATGGGGTTGGGAAACCCTGGAGAGAAGTTCAGAGGAACTAGGCACAATGTAATTATTCTACCATTTTTCGTTGTTCATCTTGAATCACATTTTGATTTTGTAATGAGAGGTATTCGTTCAGTCATTCTTATTTTGGTTCAGTGCAGGTGGGCTTTGAGATGATTGATGTGTTTGCTGAGTCTATTGGAATTTCAATGAACAGGGTTCACTGTAAAGCTGTGTTTGGTGAAGGTATGTTCTCTTTTTATGTTATAATTTTTTTCGTTTCATCTGTTAAGTGTTTTCAGGTGTTATTGTTGTTTGATTCCATGAAGTTCAAAGATATTTTGGCTATTGATGTCAGGTTTTGTTGGAGACGTCCCTGTTTTTCTGGCAAAGCCTCAAACTTACATGAACCTGAGTGGTGAATCTGTATGTGATTGAGAAACTTTTTTTGGCTTCTGTCTTCTTATAAGTTGGGTTCCTTGTAGTCTTAGACTCTTAGGTTTTTATTATTAGAGTTCATAGATTCATTTGAGTGAATTCATCTTGCAAAATTATGTCGACTTTGAAGGGGAAGAAGCTGGTAGTCGTTCTAAAATCCACTAGGAAGTTGGGAATACAGTTCCTGTGAAGGCTAATGTAGCTAGTTCAATACATGTCTATGATATCTTCTTTACCGAGATTGGTTTGATATTGTAAATCTTGTTTTTTTCATCATACTGCAGGCAGGACCACTTGCAGCTTATTACAAGCTACCTCTGAACCGTGTACTTGTGGTAAATCCTTATGTGCATTTCAGCATTTTTTTTTTCTCATTAGAGTTCTTAAATGAACAGTGCCGCGGTATTGTTTTGAGTATTGTTTTGAGTATTTGACTGCTGCAGTTTCACGATGACATGAACTTACCTTGTGGGGTGCTTCGGCTTCAACACAATGGAGGACACGACTACCACAAAGGGTATCATTGAAACTATTTTATTATTTTTTTTGTTTTTTATATTTTAGTCGTCTTCTATTGTAGCCTATCTCCACTCTATAACCCCAATCTAATTGCCCTTTGTCTCTACACCTCTTACATTAACATACATTTGACTAGTTTTTTATAATAAGAAAATGAACTTTCACTGAGAAAGAAATGAAATCACAAGGGTGTCCAATAGAAAAATACCCCAGGAAAGGAGACTCAACTAAATAAAAGGGCTCCAACCAACATCAAGTAAAATAAGACCAATAATCACAAAGAGGCATTACATCGGTGCCCAAAGAGAAATATTGAATCTGTCAAGGGACCAAGCATCAATAGGTGCTTCTCAAGGCTCTCTAAAAGTCCCGTTTCTCTTTCCCCTCAAACTCCACATCACAAAACAAATCCTTTCTTGCTTAAAAAACTTCATCTTCTGAACAAAAGCTGGATGAAGAAGGAACTCCTTGCTCATCTCCTCATAACCTCTGTGTCTAAAACATTGAAAGCCAAACACATCAAAAAAGGGATGCCACTCTTAACAAAGTCACAGCCTCCAAGGAATATGATTATGATCTTCCTCCACTCTCCGGCAAAGAATCCAGTGAAAAAGCCCAATCAAAGCAGACATCTTTCTTGCAAGCTGATCTGAAGTGTTAACCCTTCCATGGATGACCTGCTTGCCACATGAACTGAATCTTTGGGATTTTTACCTTCCGTAGATGAAATCGATTAAGCTATTTTCATTTGAAAAGAAAAACGATCCAATAGGAGTTTGTAAGATTATTTATTATCTCGGAGTTAGCATCCAGCATGCAAGAGTGATGCTGATCAGATGCATCCTATTTGAATTTCTTAGTTATGTTCGATCATGTCAGTGAATTGCTCCATTAGAGGCTCTTAAAGAATCTAAGCATAGCATTTGGGGGATCAAGATGTTGTACATCACCTTCTAATACGACTATCGAATGCTATTGCCTCAAAATAAAAATAAAAAAAACCTTGAATGCTATCTATGTCCACAAAGTTTTTTCAACACAATTCCTCTAAATATTACATTAATCCGGCATGCTACAACTTTAACTTTATATATTTTTTTGAGACATACATTTTATTGTCCATGCTTGTAAATTTCCTGGCAGTATTCTTTCTTTTAGTAAAACCTTCACGACTTCTATTCTATGTTGCCTTTCTGGTACATCTTGAAATTGATTTCTATTTTAGGCTGAAGAGTGTCATTTATCATTTTCGAGGGAACAGAGAGTTTGCTAGGTTAAGAATTGGTAAGTTTTGTTGCCTACGCTATTTTGTAGTTTTTTTTATGTTCTCTACTCTTAGAACATTTTTCTTTCTAGGCATTGGAAGGCCACCTGGTCAAATGGATTCCAAAGCATTCTTGCTTCAAAAGTTCAACAACACTGCTCAAGAACGAGTGAGTCTTTTTGCTGATTATAATATACAAAACTTATGGTTGAAACCTTTCTATTTTTCTTTGTTTCATTGAGCCCCCACGTGTTTCATTCACACCACAACTTCCTTGTTTTGCTTATATGTCATCTCTCGCTACCATTTTTTTGCTAAATGTGCACACCTTTGAAAAATATAGTTTGGATCCTTTTCCTGAGATGTTTTCAAATATGAAAAATTAAGTCAAATTATTTACAAGTTAAAATCTATCACTATCTGTTGTTGTCCGTCAATGATATACAGTGACATTTTGATATATTTGTAAATATATTTTAATAGTTTTGTCATTTAAAGCAATTACCTCTCTTAGTCTTCTTTTTATTTTTTGGTAAGTAGGTTGTATCTTCACATCAGTTATTCCTTTTGCTAACTCATTGCGAATAATGCTTGATTACATTATGCTTAATTAGTATCATCCTAGTTTAACAATACCTTTATTTTTGTTTAAAAAGATTGATACTGCATTACAAGAAGGGGTTGGCGCATTGAAGCTCCTATTGTCTCACGGTTTGTCCGAGAGTGCAAGACACTTCAACCACAAACAAAAATACAAGCATATAAGGCTACAAACCATGCCAGTATGATGAGACTATATGGTGCATATTGCGAATTGCCATTGCTAACGAATCATACAAATTCTTCCAAGTCCTCACAGATAAAAGGAACGAAGGCAGAGTGGAGCTTCTACTTCAGGTACACAATGATTTTGTCTTCTGATATTTATGTAATTATTTTACAGCCGATTGCCCTCTTGCATTGTGCTTCAATTTCATATCTTTAAAGTTATATAAAAAGAATTTGGGATATGACTACCAATTTTCCTGTGTGATTATTCCAAGTCTGGAGGATTCTAATTCAGTTAAAAGACTAATATACCCTTTCTTTTTGGTATTTTACCACCCTAAACCATATATCTGACCCAATCATTTTTGGATGTCAAACTAACTCATAGGTTGATACAAAATTCTAGATAGGTAACCATGGTGGATTTGACTCGATCTCTTAAGTTCTTTATTAAATTATATCTATGACCCAGTTTGACTACTGTACTAACCCATGATGGTTTTTTTAAGAATTCGTTTGTAGGAAGACTTTGTTAGATGAAACTGTAAATGGGTAGGGGAAGGTTGAAAAATAGACACAGAGCACACACATATAGGTATTATGTACCACCATTTTACATGGCTTTTTATTTTTCTAAAGTCTTGATTTCAGTTGGGGTGAACCTAAACCCAAAAACACTGAACAGAACCAAACCAAACCAAAAATTCAATTTTTATTGAAGCATTGCTTTGGTTCGGCTTGAACTGAAAATTTAAATAACAAGATAAGATTTAAAAATCAGTTGTTAAACTGGATTGGTTCCCAATTAATTTTCTTTTTCTATTTGTCCAAATTAAACTTCAAATTTTGTCATTTATCTAGTCGCCCTTGTAATAAAAAAGAATTTGGTGAATGGGCTAAAAGGAATGACTTCTAAGGCTTGAAACATGAGAGAATGAAATGTTAGCAATATCTTGGTGGCCAACTACTACTTCCTCCACCAACTTTGTGGCTTTTACTACCAATCACATATTAGAGTTCTTTTCTTTCACCCTCTTTTTCTCAAATAATAATAATGGATAAATATTATTGAAAAAAACTTAGGTACATTCGAGTTACACTAATCTCTCGTGTAGATAATTAAAATATACCATTTTGGGGCCGTTGTATGTATTGCTCAACATTATTCACTATTTGAGTTATAATACCATTTTCAGTTATTGTACTTTAGAGCTTGTTTGATCCCTATACTTCTCAAATCCATATACTTTTTCAAATGTTCAATTTTGTTTTTCAAATGTTCAATTTTGTTTTTGTAGTTTTAATAAATTTTAACTTATTTTTGGAATAATATCAATAATTTTCCGGATGAGAAATACAATGTAATTATATTCTAAAATTTGCAGTGAAAATGCTAATAGTAAGAATAAGATTTGAGAAAAATAACAAAAAAAAAACTAACCATCATATTAAAATTAGATACATGAAAGTACATAAACTAAAACTAGATAACTAGATAACTTGATGGTATAATTTAACCAAACAAGTGCTATGTAATATAATTCAAAGGGGAGTTTGTAAAAATAGCAAAAACAATTTATGGTAATAGAGTTCATGTTACTTACATTTTTTAAATTGTAAAAATTGTAAATTTAAAAGCAGATTATCGTCATATTACTATCTGATTATCGTTATATTCATCAAATTTGTAATATGCGAAAAAGAGGTGTTATGAACTGTTTTTTTCTATTTTTTTTTGTCATTTAATACAATTTTTCTAATTTAAATGTTTTTATTTTATCAATAATGTAATGAATTATTCACGTGCTAATTTTAACTCTCTCTCTTCATAAGCTTAGTGGTTTAAATAAAATCAAGCTTTTGCACAAATAGCGATGATGATTGACAAATTGACTTCATCATAAACTCACAAAACCTAACAAAAGAAAAGAAAAATACATCTCGTTTTCACTTCAAATCTTAGTAACATTTACATATATGTTTTAAATATGGTTCATTCAACGTAGAAATGTGTTGTTTTCAACGAATCCTCGAAAAAAGAAAAAAATCATTATATGAAAACCGTAGGATTTAAAAGTTAAGAACCCAATTCCTGAATATCTAATATCAAATTCTAAAACTTTCACGTCAATTTTGACAACAATTCAAAACAAAACGACTAAAATTTGATTACTTTTTGATTGAAATACTAGTAGTTGGAAATGCTGGTGATGAATCTCCATCATATCTCATTCCAAATTCAATATATGTCAAAAAAAAACTTTTACAACCTAATAGCCAAACTTAAACAGTCTTTCCTATAAAAACCCTAATTAATCAAAGGACTAAACTAATCATAATTAAGTGTCATAAAAATGATACTAAAATTAGATCAAATTGATCAAATCCAATCTAATTAATAATTTGATGCATCAACCAATCATATCAGGGAAATTTGTCAAACTTTTTCAAATTTAAAGTTAAAATGGCAATACTTTTGTATATAAATGTGTATACAATTCTATCTTTTTTGAATCATCAACTACACATCTACCCAAATTTTGACAGTGAGTTTTCTTTCAAGGTTAAAATATTAACTTTAGTTGTTTTATTTATTAAAATAAAATTTTAGAAAATTACGATCGATAATCATTTTAGTAATAATAATTAAGAATACATCAAATTTTTTGCAAATATAGCAAAACTATCATCCGACTTGTATCGTTGATAGACTTCGTATGGTCTATAAGTGATGGACTGATATTTGTAACATGATCTATCAGTGATAAACTTATATCATTGATAGAATTTGACAAATTTTACTATATTTGCAAATTTTTTAAAATGTCGTTATATACTTAATTAATTTGAATCTAATTGTTAAATTTGCACCTATCCCTAAAATTTGTAGGTGAAAAACTTAGATATAACCTTTTTCACTAAACGTCAAAGATCCATTTTTTTAATGGATGAACAAACTAATTACTTATTTAAGAGCTTAGTTTGTCCATTATGTTTCTAACTATTCATAATTTGAATTTTAAAATTTAAAATATATATCATATAATGTATTAGAAATCATGTTATATTCTAAATTCATCCTTTGGTTGGGATGAAGTTAGTCCACATGGTTTAGTTATAATGATTTGATACAACTTCGTTAATTACTGTGAGGACTATTTACGAAACGTTTATCTAAATTTTAATTATTGATCGTGAGATCTAATTTCATAAATAAATTAATACTCGTTTACTGTTCACTTGAAAAACTACTTTTAGTTTTCAATTGTTTAAATTAGTATTGGTTTTGTAAATTTTGCCACTAAATACGTAGGATTGGATGAAAATGGGAAACAAAAAGTTTTCTTTTTCATAGTCTACCAATGACATTCTTAATAATATCAATTTTTTTTAGCTAAACATTTGTTATTACTGAAACGAAGTTAGAGCTAATGGCTGTAAAAGAAAGAAGGAAGAACATAAGGATGGCTTGGCACAAACGACCGTAATAACCCGGCCTTGATTTTGGATCTTGCGGGTGGGTTTGGTGGTTGATGTGAGAGAGAGAGAAAAAAATGAGGATCTCATATCAAAAAATGATGGGTTCGTTTTTGGTGGAGATGTGAGAGAATGAGAAAAATCCGAGTATAAATGACCCAACTTCCACTGAAATCGCTTGAGCCCTTTGCAATATCATCGTCTTCTTCTTCTTCTTCTTCTTCATTCTCTATTTTTGCACTGAGAAGAGGAGCGATTGCCCCATTTTCATATCTCCTTTTCTCCTCTATCCATCATCTTCCTTCCCTTCGCTTATCAATTCGCACTACTTCCACTGTTGTCAGGTATTTCTTTCACTCTCTGGTGCATTTCTGTGTTGTGGAGTCTTTTGGGGCTGTTTTTTGTATCGTTTTGTGATCTGGGTATGGATTATGTTAGCTATTTTTGTGGTTGATTGTTTGGAATTTACTTTATATCCTCTAATGGAAATGTGGGGGGTTTTTGTGAAATGGTCTTAGCTGATTGGTAATAGATGAACATGAAGGGCTCTGACTTTTTGTTTCGTTTTCTTTATGTTATTCCTTAGAGGCTTTGAGTTCAAGTAGGTCTTTAGTTTCTTTTACTTTTTTTTCTCTGTTGTTCTTTGATTTGGCTAAACATGATTAAGCGGCTGACGTTTCTCTTTTCTTCTTCTTTTTGCTTTAATGCCGTTTTGGTTGTTCTGTTATGGCATTTTTGTACTGATACAAAGTCCATAAGATTCGAACTAATGAGGTTTTTCATTTAGATATTTATGATTGGTTCTTCATATTAAAATTCTACATGTTGATTGCTGATTTTGTGAATTACGTGTGCCATTTTTTGAAATGAAATGAAACCGTTAATGTTTTTTCAGAAAATTTAGTTCGCATTAACATTTAATGGTGTGTCTATTTTGCTAATGGTATTCTTTCATGTATGCATAAAATGAAAAATTGTGTTCTTTTTAACGGTTTATACCATTACGTATGGCAAACAAGTGAAGTGATTCTGTTAACAACATAGTAACTTAAAATCATAATCACCATGGGTTGTCACAGTGGTCATTCTAAGGGTTGGTAAATAGAGGTTTTATAGAGAATGAGTTTGGTTTTATAGAGAATGAGTTCAAACCATGTTGCTACCTATTTAAGATTTAATATTTTACGAGTTACCTCGAGAGCTTAGTATGTAGCATGAGAGTAATCGAGGTGTCCACAAGGTAGTATAAAAACTTAATTTTTTTATCGTCTCCTTAAGTTGGCCTTGGTTGGGAAAAGCCTCCTAGAAATTAGTGAGGTGGATTGTGAACAAGGAATAGGTTTTCAATACCTCCATTAGCAAAAGAAAACTTATCTTATATTAGAGGAATATGGGAAATTGTTAATGAAAATGTTCATTGAGTTCTACTGACATTTCATTTTTATATTATTTTCTTGTTAATATGTAAAAGAGCAATGGCATCCCACATTGTTGGTTATCCTCGCATTGGTCCGAAGAGAGAACTTAAATTTGCCTTGGAGTCTTTCTGGGATGGGAAGAGCAGTGCTGATGACTTGCAGAAAGTGGCTGCTGATCTCAGGTCATCTATTTGGAAGCAGATGGCTGAAGCTGGAATCAAGTTTATTCCAAGCAACACTTTTTCGTATTATGATCAAGTGCTTGACACTGCAACTATGCTCGGAGCTGTTCCGTCAAGATATGGATGGAACGGTGGTGAGATTGGATTTGACATCTACTTCTCCATGGCTAGAGGGAACGCTTCAGTTCCTGCCATGGAAATGACGAAGTGGTTCGATACCAATTAGTAAGTGAAAAACTCTATGCATTTCCCTACATTTTTATGATAATACTATTTGTAAAACTTCTACTAGTCAATGATTTGAGGAATGTTAACGATGTGTGTTCTGTTACAGCCATTACATTGTGCCCGAGCTGAGTCCAGAAGATAAATTTACTTATGCATCTCACAAAGCAGTAAACGAGTTCAAGGAAGCTAAAGCTGTAAGATTTCTTATTAAAAGAAATTAGGGAAAAAAAAAAAAAACACTTAAAAATGAACTTATTTTCTGAGTTTGGATAATGGTGGTAGATTCAAGTTTCATGTGAATAAGATTAATTCAAGGAAGGATGTACTTAACTGGTTTGCACTTGTAATTTTTTTTTAATGTCCACCAAGAAAAAGTTTAAGAATTTTCTTGCCCTATGAACAGTAGTACAAGTTAACTTGAATCATCTATCCTTGAACTTGACAACAAACATCCTTTCCATTTCCAGTTTGATTAATTTTTTGCTTTCTTTGTCGTCTGTTAGTTCTTCTGATTCAGTGTTTTAATTTATTATTTCAATTTTTTTTTTATCATTTGACCTGCCCTACTGATGCATTTAACTTCTTTTATACTCCATCTTATATGATATTATATTTCTTATATTTAAAGAGGCATCTTGCTTTTATCATGCGACAGAACCTTATTTTTTAGCCATTCATAATAACAAGCAAGATGCCTTATTCGGACTGACATGCCTTTCAATTTAAGAATTGTTTCAAGTGAAGAATATTTGAATAAATGCCTGGATAGTAAATTATATTCTATGAGAATTTTATGATATTTGAAGTTCAGTCATGAAATTTCAATATGAGCATGTTAAGCATTTTTATTACCTTTTTTCCTTTACACTCCCACTTTTCTGTGCAGCTCGGAGTTGAGACTGTCCCGGTTCTTGTAGGCCCTGTATCCTTCTTGTTGCTTTCAAAACCAGCCAAGGGAGTTGAGAAATCCTTCTCACTTCTCTCCCTAATCGACAAGATTCTTCCTGTTTATAAGTCAGTATTTCTTTTCCTCTTTCCAAAGTTCGTCTAGTTTACGTATTAACCATTTCATTTCAGTTCCACTATCTTCTTATCAACTCTTATTTGTACCTGCAGGGAAGTTGTTTCAGAGCTGAAGGCTGCTGGTGCCACTTGGATTCAATTTGATGAGCCTACACTTGTGAAAGATCTTGACGCGCACCACTTACAAGCATTTGCTCAAGCCTACTCAGAGCTGGAATCCACTTTATCTGGTTTAAATGTTCTGATTGAGACATATTTTGCTGATGTTCCTGCTGAGGCATACAAAACTCTCACGTCTTTGAAGGGTGTCTCTGGTTATGGATTCGATCTAGTTCGTGGAACACAGACACTTGATCTGATCAAAGGTGGATTTCCTTCAGGCAAATATCTCTTTGCCGGAGTGGTTGATGGAAGAAACATATGGTCAAATGACCTTCAAGCTTCCATAACTACCTTGGAGGCGTTGGAAAACGTTGTTGGAAAAGGTGCTTGTTTCTTAACGTGCTAGCACATTTTATATTAGCGTGGATACATTTTTAGACTATAAACAGTGGTAATATATGCTGTTCTTCTGTCCTTTACAGAAAAAATTGTCGTCTCCACTTCCTGCTCTCTTCTTCACACTGCTGTTGATCTGGTGAACGAAACAAAACTAGATGATGAAATTAAGTCCTGGCTTGCTTTTGCCGCTCAGAAAGTTGTTGAAGTTAATGCCTTGGCTAAGGCACTCTCTGGACATAAAGATGAGGTACACGAAGCAACCTTATTTTTGACCTTGCAATAGGAACTTAGGGGCAGGGTGTTGTACCTTAAATCAAACAATGTAGCCTTAGTGCATAGTCTTTGTAGACTCATGGTACAAACGAAACATGTGTGTGTGTATATAATAAAATAAATGAAGCTTGACTTATTTGACATGAAATGGAAATCTATTGTTGCGGAACTGAAGATACTAAAATTTGAGCTTATAACTTTTTTCCTTTTAGGCAAAGAGAAAAATGAGCTTCTAACTGTCGCTTCTTCTATTCCAGGCATATTTTTCTTCCAATGCTGCTGCTCAAGCTTCAAGGAAATCATCCCCAAGAGTGACAAATGAGGCAGTCCAAAAAGCTGTATAAATTATTTGATGTACTAATATCATTTTGTAGGATCTCTCTATATTCTTGCATATCGTGTGCTTAACCTTCTATGTCGACTTTACTTAGGCTGCTGCTCTGAAGGGCTCTGACCACCGTCGGTCAACAAATGTAAGTGCCAGGCTGGACGCTCAGCAGAAGAAGTTGAACCTTCCAATGCTGCCTACAACAACAATTGGATCCTTCCCTCAAACTATGGATCTTAGAAGAGTGCGACGTGAATTCAAAGCAAAAAAGTATGCCTTTTTGTCTTCAGATATAATTGTTCGTCCTCAAAAAAGTACATTGTTGTTAATTTTCTAATACACTTGCATCCTGTTCCTTTAGAATCTCTGAGGAAGACTATGTCAGCTCCATCAAGGAGGAAATCAACAAAGTTGTTCAGCTCCAGGAAGAGCTTGACATCGATGTTTTGGTGCATGGAGAGCCTGAGGTAAGCATTTGTCTTCAAACAATGTTGAAGAAATTTAACTAACCATACATTGTCTAAGTTTAAACTGTAGCATCTGAATGTTAACGTCTCAGAGAAATGATATGGTTGAGTACTTTGGAGAGCAACTGTCTGGCTTTGCCTTCACTGTCAATGGTTGGGTCCAATCCTATGGGTCCCGTTGTGTCAAGCCGCCTATCATCTATGGTGATGTCAGTCGTCCAAAACCCATGACCGTCTTTTGGTCGACAATGGCTCAGAGCATGACGAAGCGGCCTATGAAGGGAATGCTTACTGGTCCAGTTACAATTTTGAACTGGTCTTTTGTTCGAAATGACCAACCAAGGTATGCGAATTTAATGAATCTCAGTTGCTGTTTCTTTGATCGTCTGTTTTATTGAGGGCAGTTCATGGTAGTTAATTCAATTGTTTTTCATAGGTTTGAAACATGCTACCAAATTGCCTTGGCAATCAAGGACGAGGTTGAGGATCTTGAGAAAGCTGGTATTACAGTCATCCAGATTGATGAAGCAGCACTGCGAGAAGGTTTGCCTCTCCGAAAGTCCGAGGAAAATTTCTATTTGAATTGGGCTGTTCATTCATTTCGAATCACCAATTGCGGTGTTCAAGATACCACCCAGGTCCTTTCCTACTCTCTTTTGCTTAAAAGTCCGATTATTTCTCTCACTAAATCAATTTGTTAGCGTTGTTTCATGTCATTTGCATTGAGTCGTATAACCGTCTTTTCAATGACATAGTGAACTTTATGATTTTGATGACTGCAGATCCACACCCACATGTGCTACTCGAACTTCAACGACATCATCCATTCGATAATCGACATGGATGCTGATGTGATCACCATTGAGAATTCCAGATCAGATGAGAAGCTCCTGTCTGTCTTCCGTGAGGGAGTAAAATACGGTGCTGGAATTGGACCAGGAGTCTACGACATCCATTCTCCCCGTATTCCCTCTACTGAGGAAATTGCTGACAGGATCAACAAGATGCTCGCTGTTCTTGAAAGCAACATCCTCTGGGTGAACCCTGACTGCGGTCTTAAAACCCGAAAGTACACTGAAGTCAAGCCTGCCCTCCTCAACATGGTTGCTGCTGCTAAGCTCCTTCGCTCCCAATTGGCCAGTGCCAAGTGAGTTTGAGAATGCTGTGGCAAACCTTAGGAAGAAAAACACTTAAAATTTTTCAATAAGATTTTGGCCTTCTGGTGAAGTGATCTTGTTGTTCTCTTTCTTGGAGTGTCTTCAATTTCTTAGGGAAGTAATGAACAATGTCGTCAAATTCCCATTGTGGCTTTTGTTTAAAAAGTTCAAAATTTTCTGTTTTTTTAATCAGTTTTTCTCGTTCAAGCCATTTTTTTTTGGTTTGTTTTGTAAAATAGTGGAGATGAGAATTAATGATATTAATAGATATACTTGTGTTTTGTCACACTACTTTGGGATAAAAGTAAAAGCAACAATTTAAAAGTATATTTTGTTTCCTGCATAATGATTCAGTTTCTATACTAAATTGTTACCGATTTAAGTTTACAGAGTTAAAAGTTTTGGGATCA

Coding sequence (CDS)

ATGACCCAACTTCCACTGAAATCGCTTGAGCCCTTTGCAATATCATCGTCTTCTTCTTCTTCTTCTTCTTCATTCTCTATTTTTGCACTGAGAAGAGGAGCGATTGCCCCATTTTCATATCTCCTTTTCTCCTCTATCCATCATCTTCCTTCCCTTCGCTTATCAATTCGCACTACTTCCACTGTTGTCAGAGCAATGGCATCCCACATTGTTGGTTATCCTCGCATTGGTCCGAAGAGAGAACTTAAATTTGCCTTGGAGTCTTTCTGGGATGGGAAGAGCAGTGCTGATGACTTGCAGAAAGTGGCTGCTGATCTCAGGTCATCTATTTGGAAGCAGATGGCTGAAGCTGGAATCAAGTTTATTCCAAGCAACACTTTTTCGTATTATGATCAAGTGCTTGACACTGCAACTATGCTCGGAGCTGTTCCGTCAAGATATGGATGGAACGGTGGTGAGATTGGATTTGACATCTACTTCTCCATGGCTAGAGGGAACGCTTCAGTTCCTGCCATGGAAATGACGAAGTGGTTCGATACCAATTACCATTACATTGTGCCCGAGCTGAGTCCAGAAGATAAATTTACTTATGCATCTCACAAAGCAGTAAACGAGTTCAAGGAAGCTAAAGCTCTCGGAGTTGAGACTGTCCCGGTTCTTGTAGGCCCTGTATCCTTCTTGTTGCTTTCAAAACCAGCCAAGGGAGTTGAGAAATCCTTCTCACTTCTCTCCCTAATCGACAAGATTCTTCCTGTTTATAAGGAAGTTGTTTCAGAGCTGAAGGCTGCTGGTGCCACTTGGATTCAATTTGATGAGCCTACACTTGTGAAAGATCTTGACGCGCACCACTTACAAGCATTTGCTCAAGCCTACTCAGAGCTGGAATCCACTTTATCTGGTTTAAATGTTCTGATTGAGACATATTTTGCTGATGTTCCTGCTGAGGCATACAAAACTCTCACGTCTTTGAAGGGTGTCTCTGGTTATGGATTCGATCTAGTTCGTGGAACACAGACACTTGATCTGATCAAAGGTGGATTTCCTTCAGGCAAATATCTCTTTGCCGGAGTGGTTGATGGAAGAAACATATGGTCAAATGACCTTCAAGCTTCCATAACTACCTTGGAGGCGTTGGAAAACGTTGTTGGAAAAGAAAAAATTGTCGTCTCCACTTCCTGCTCTCTTCTTCACACTGCTGTTGATCTGGTGAACGAAACAAAACTAGATGATGAAATTAAGTCCTGGCTTGCTTTTGCCGCTCAGAAAGTTGTTGAAGTTAATGCCTTGGCTAAGGCACTCTCTGGACATAAAGATGAGGCATATTTTTCTTCCAATGCTGCTGCTCAAGCTTCAAGGAAATCATCCCCAAGAGTGACAAATGAGGCAGTCCAAAAAGCTGCTGCTGCTCTGAAGGGCTCTGACCACCGTCGGTCAACAAATGTAAGTGCCAGGCTGGACGCTCAGCAGAAGAAGTTGAACCTTCCAATGCTGCCTACAACAACAATTGGATCCTTCCCTCAAACTATGGATCTTAGAAGAGTGCGACGTGAATTCAAAGCAAAAAAAATCTCTGAGGAAGACTATGTCAGCTCCATCAAGGAGGAAATCAACAAAGTTGTTCAGCTCCAGGAAGAGCTTGACATCGATGTTTTGGTGCATGGAGAGCCTGAGAGAAATGATATGGTTGAGTACTTTGGAGAGCAACTGTCTGGCTTTGCCTTCACTGTCAATGGTTGGGTCCAATCCTATGGGTCCCGTTGTGTCAAGCCGCCTATCATCTATGGTGATGTCAGTCGTCCAAAACCCATGACCGTCTTTTGGTCGACAATGGCTCAGAGCATGACGAAGCGGCCTATGAAGGGAATGCTTACTGGTCCAGTTACAATTTTGAACTGGTCTTTTGTTCGAAATGACCAACCAAGGTTTGAAACATGCTACCAAATTGCCTTGGCAATCAAGGACGAGGTTGAGGATCTTGAGAAAGCTGGTATTACAGTCATCCAGATTGATGAAGCAGCACTGCGAGAAGGTTTGCCTCTCCGAAAGTCCGAGGAAAATTTCTATTTGAATTGGGCTGTTCATTCATTTCGAATCACCAATTGCGGTGTTCAAGATACCACCCAGATCCACACCCACATGTGCTACTCGAACTTCAACGACATCATCCATTCGATAATCGACATGGATGCTGATGTGATCACCATTGAGAATTCCAGATCAGATGAGAAGCTCCTGTCTGTCTTCCGTGAGGGAGTAAAATACGGTGCTGGAATTGGACCAGGAGTCTACGACATCCATTCTCCCCGTATTCCCTCTACTGAGGAAATTGCTGACAGGATCAACAAGATGCTCGCTGTTCTTGAAAGCAACATCCTCTGGGTGAACCCTGACTGCGGTCTTAAAACCCGAAAGTACACTGAAGTCAAGCCTGCCCTCCTCAACATGGTTGCTGCTGCTAAGCTCCTTCGCTCCCAATTGGCCAGTGCCAAGTGA

Protein sequence

MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTSTVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDGRNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK
Homology
BLAST of Cucsat.G7170.T1 vs. ExPASy Swiss-Prot
Match: Q42699 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Catharanthus roseus OX=4058 GN=METE PE=2 SV=1)

HSP 1 Score: 1358.2 bits (3514), Expect = 0.0e+00
Identity = 674/765 (88.10%), Postives = 728/765 (95.16%), Query Frame = 0

Query: 66  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 125
           MASHIVGYPR+GPKRELKFALESFWD KSSA+DLQKVAADLRSSIWKQMA+AGIK+IPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDKKSSAEDLQKVAADLRSSIWKQMADAGIKYIPSN 60

Query: 126 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 185
           TFSYYDQVLDTATMLGAVP RY + GGEIGFD YFSMARGNASVPAMEMTKWFDTNYHYI
Sbjct: 61  TFSYYDQVLDTATMLGAVPPRYNFAGGEIGFDTYFSMARGNASVPAMEMTKWFDTNYHYI 120

Query: 186 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 245
           VPEL PE  F+YASHKAVNE+KEAK LGV+TVPVLVGPV+FLLLSKPAKGVEK+F LLSL
Sbjct: 121 VPELGPEVNFSYASHKAVNEYKEAKELGVDTVPVLVGPVTFLLLSKPAKGVEKTFPLLSL 180

Query: 246 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLNVLI 305
           +DKILPVYKEV+ ELKAAGA+WIQFDEPTLV DL++H L+AF +AYSELESTLSGLNV++
Sbjct: 181 LDKILPVYKEVIGELKAAGASWIQFDEPTLVLDLESHQLEAFTKAYSELESTLSGLNVIV 240

Query: 306 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDGRNIWS 365
           ETYFAD+PAE YK LT+LKGV+G+GFDLVRG +TLDLIKGGFPSGKYLFAGVVDGRNIW+
Sbjct: 241 ETYFADIPAETYKILTALKGVTGFGFDLVRGAKTLDLIKGGFPSGKYLFAGVVDGRNIWA 300

Query: 366 NDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVVE 425
           NDL AS++TL++LE +VGK+K+VVSTSCSLLHTAVDLVNE KLD EIKSWLAFAAQKVVE
Sbjct: 301 NDLAASLSTLQSLEGIVGKDKLVVSTSCSLLHTAVDLVNEPKLDKEIKSWLAFAAQKVVE 360

Query: 426 VNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSARL 485
           VNALAKAL+G KDEA+FS NAAAQASRKSSPRVTN+AVQKAAAAL+GSDHRR+T VSARL
Sbjct: 361 VNALAKALAGEKDEAFFSENAAAQASRKSSPRVTNQAVQKAAAALRGSDHRRATTVSARL 420

Query: 486 DAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQEE 545
           DAQQKKLNLP+LPTTTIGSFPQT++LRRVRRE+KAKKISE+DYV +IKEEI+KVV+LQEE
Sbjct: 421 DAQQKKLNLPVLPTTTIGSFPQTLELRRVRREYKAKKISEDDYVKAIKEEISKVVKLQEE 480

Query: 546 LDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTV 605
           LDIDVLVHGEPERNDMVEYFGEQLSGFAFT NGWVQSYGSRCVKPPIIYGDVSRP PMTV
Sbjct: 481 LDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPNPMTV 540

Query: 606 FWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGIT 665
           FWS  AQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGI 
Sbjct: 541 FWSQTAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGIN 600

Query: 666 VIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSI 725
           VIQIDEAALREGLPLRK+E  FYL+WAVHSFRITN  +QDTTQIHTHMCYSNFNDIIHSI
Sbjct: 601 VIQIDEAALREGLPLRKAEHAFYLDWAVHSFRITNLPLQDTTQIHTHMCYSNFNDIIHSI 660

Query: 726 IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLA 785
           IDMDADV+TIENSRS EKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLA
Sbjct: 661 IDMDADVMTIENSRSSEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLA 720

Query: 786 VLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 831
           VL++NILWVNPDCGLKTRKY EVKPAL NMV+AAKL+R+QLASAK
Sbjct: 721 VLDTNILWVNPDCGLKTRKYAEVKPALENMVSAAKLIRTQLASAK 765

BLAST of Cucsat.G7170.T1 vs. ExPASy Swiss-Prot
Match: Q2QLY5 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0623900 PE=2 SV=1)

HSP 1 Score: 1352.4 bits (3499), Expect = 0.0e+00
Identity = 668/766 (87.21%), Postives = 726/766 (94.78%), Query Frame = 0

Query: 66  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 125
           MASHIVGYPR+GPKRELKFALESFWDGKSSA+DL+KVA DLR+SIWKQMA+AGIK+IPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDGKSSAEDLEKVATDLRASIWKQMADAGIKYIPSN 60

Query: 126 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 185
           TFSYYDQVLDTA MLGAVP RY W GGEIGF  YFSMARGNA+VPAMEMTKWFDTNYH+I
Sbjct: 61  TFSYYDQVLDTAAMLGAVPERYSWTGGEIGFSTYFSMARGNATVPAMEMTKWFDTNYHFI 120

Query: 186 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 245
           VPEL P  KF+Y+SHKAVNE+KEAKALGV+TVPVLVGPVS+LLLSKPAKGVEKSF+LLSL
Sbjct: 121 VPELGPNTKFSYSSHKAVNEYKEAKALGVDTVPVLVGPVSYLLLSKPAKGVEKSFALLSL 180

Query: 246 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLNVLI 305
           +  ILPVYKEV++ELKAAGATWIQFDEPTLV DLD+H L AF+ AY+ELES LSGLNVLI
Sbjct: 181 LSSILPVYKEVIAELKAAGATWIQFDEPTLVLDLDSHQLAAFSAAYTELESALSGLNVLI 240

Query: 306 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIK-GGFPSGKYLFAGVVDGRNIW 365
           ETYFAD+PAE+YKTLTSL  V+ YGFDL+RG +TLDLIK  GFPSGKYLFAGVVDGRNIW
Sbjct: 241 ETYFADIPAESYKTLTSLNSVTAYGFDLIRGAKTLDLIKSAGFPSGKYLFAGVVDGRNIW 300

Query: 366 SNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVV 425
           ++DL AS+TTLE+LE +VGK+K+VVSTSCSL+HTAVDLVNETKLD EIKSWLAFAAQKVV
Sbjct: 301 ADDLAASLTTLESLEAIVGKDKLVVSTSCSLMHTAVDLVNETKLDSEIKSWLAFAAQKVV 360

Query: 426 EVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSAR 485
           EVNALAKAL+G KDEAYF++NAAAQASR+SSPRVTNE VQKAAAALKGSDHRR+TNVSAR
Sbjct: 361 EVNALAKALAGQKDEAYFAANAAAQASRRSSPRVTNEEVQKAAAALKGSDHRRATNVSAR 420

Query: 486 LDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQE 545
           LDAQQKKLNLP+LPTTTIGSFPQT++LRRVRRE+KAKKISEE+Y+S+IKEEI+KVV++QE
Sbjct: 421 LDAQQKKLNLPVLPTTTIGSFPQTVELRRVRREYKAKKISEEEYISAIKEEISKVVKIQE 480

Query: 546 ELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMT 605
           ELDIDVLVHGEPERNDMVEYFGEQLSGFAFT NGWVQSYGSRCVKPPIIYGDVSRP PMT
Sbjct: 481 ELDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPNPMT 540

Query: 606 VFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGI 665
           VFWS +AQSMT RPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIK EVEDLE  GI
Sbjct: 541 VFWSKLAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKKEVEDLEAGGI 600

Query: 666 TVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHS 725
            VIQIDEAALREGLPLRK+E  FYL+WAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHS
Sbjct: 601 QVIQIDEAALREGLPLRKAEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHS 660

Query: 726 IIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKML 785
           II+MDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADR+NKML
Sbjct: 661 IINMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRVNKML 720

Query: 786 AVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 831
           AVL++NILWVNPDCGLKTRKY EVKPAL NMV+AAKL+R+QLASAK
Sbjct: 721 AVLDTNILWVNPDCGLKTRKYNEVKPALTNMVSAAKLIRTQLASAK 766

BLAST of Cucsat.G7170.T1 vs. ExPASy Swiss-Prot
Match: O50008 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=MS1 PE=1 SV=1)

HSP 1 Score: 1349.0 bits (3490), Expect = 0.0e+00
Identity = 668/765 (87.32%), Postives = 719/765 (93.99%), Query Frame = 0

Query: 66  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 125
           MASHIVGYPR+GPKRELKFALESFWDGKS+A+DLQKV+ADLRSSIWKQM+ AG KFIPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDGKSTAEDLQKVSADLRSSIWKQMSAAGTKFIPSN 60

Query: 126 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 185
           TF++YDQVLDT  MLGAVP RYG+ GGEIG D+YFSMARGNASVPAMEMTKWFDTNYHYI
Sbjct: 61  TFAHYDQVLDTTAMLGAVPPRYGYTGGEIGLDVYFSMARGNASVPAMEMTKWFDTNYHYI 120

Query: 186 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 245
           VPEL PE  F+YASHKAVNE+KEAKALGV+TVPVLVGPVS+LLLSK AKGV+KSF LLSL
Sbjct: 121 VPELGPEVNFSYASHKAVNEYKEAKALGVDTVPVLVGPVSYLLLSKAAKGVDKSFELLSL 180

Query: 246 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLNVLI 305
           + KILP+YKEV++ELKAAGATWIQ DEP LV DL+   LQAF  AY+ELESTLSGLNVL+
Sbjct: 181 LPKILPIYKEVITELKAAGATWIQLDEPVLVMDLEGQKLQAFTGAYAELESTLSGLNVLV 240

Query: 306 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDGRNIWS 365
           ETYFAD+PAEAYKTLTSLKGV+ +GFDLVRGT+TLDL+K GFP GKYLFAGVVDGRNIW+
Sbjct: 241 ETYFADIPAEAYKTLTSLKGVTAFGFDLVRGTKTLDLVKAGFPEGKYLFAGVVDGRNIWA 300

Query: 366 NDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVVE 425
           ND  AS++TL+ALE +VGK+K+VVSTSCSLLHTAVDL+NETKLDDEIKSWLAFAAQKVVE
Sbjct: 301 NDFAASLSTLQALEGIVGKDKLVVSTSCSLLHTAVDLINETKLDDEIKSWLAFAAQKVVE 360

Query: 426 VNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSARL 485
           VNALAKAL+G KDEA FS+NAAA ASR+SSPRVTNE VQKAAAALKGSDHRR+TNVSARL
Sbjct: 361 VNALAKALAGQKDEALFSANAAALASRRSSPRVTNEGVQKAAAALKGSDHRRATNVSARL 420

Query: 486 DAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQEE 545
           DAQQKKLNLP+LPTTTIGSFPQT++LRRVRRE+KAKK+SEEDYV +IKEEI KVV LQEE
Sbjct: 421 DAQQKKLNLPILPTTTIGSFPQTVELRRVRREYKAKKVSEEDYVKAIKEEIKKVVDLQEE 480

Query: 546 LDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTV 605
           LDIDVLVHGEPERNDMVEYFGEQLSGFAFT NGWVQSYGSRCVKPP+IYGDVSRPK MTV
Sbjct: 481 LDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPVIYGDVSRPKAMTV 540

Query: 606 FWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGIT 665
           FWS MAQSMT RPMKGMLTGPVTILNWSFVRNDQPR ETCYQIALAIKDEVEDLEK GI 
Sbjct: 541 FWSAMAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKGGIG 600

Query: 666 VIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSI 725
           VIQIDEAALREGLPLRKSE  FYL+WAVHSFRITNCGVQD+TQIHTHMCYS+FNDIIHSI
Sbjct: 601 VIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDSTQIHTHMCYSHFNDIIHSI 660

Query: 726 IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLA 785
           IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPS+EEIADR+NKMLA
Sbjct: 661 IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSSEEIADRVNKMLA 720

Query: 786 VLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 831
           VLE NILWVNPDCGLKTRKYTEVKPAL NMV AAKL+RSQLASAK
Sbjct: 721 VLEQNILWVNPDCGLKTRKYTEVKPALKNMVDAAKLIRSQLASAK 765

BLAST of Cucsat.G7170.T1 vs. ExPASy Swiss-Prot
Match: Q2QLY4 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0624000 PE=2 SV=1)

HSP 1 Score: 1347.0 bits (3485), Expect = 0.0e+00
Identity = 666/766 (86.95%), Postives = 724/766 (94.52%), Query Frame = 0

Query: 66  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 125
           MASHIVGYPR+GPKRELKFALESFWDGKSSA+DL+KVA DLR+SIWKQMA+AGIK+IPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDGKSSAEDLEKVATDLRASIWKQMADAGIKYIPSN 60

Query: 126 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 185
           TFSYYDQVLDT  MLGAVP RY W GGEIGF  YFSMARGNA+VPAMEMTKWFDTNYH+I
Sbjct: 61  TFSYYDQVLDTTAMLGAVPERYSWTGGEIGFSTYFSMARGNATVPAMEMTKWFDTNYHFI 120

Query: 186 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 245
           VPEL P  KF+Y+SHKAVNE+KEAKALGV+TVPVLVGPVS+LLLSKPAKGVEKSF+LLSL
Sbjct: 121 VPELGPNTKFSYSSHKAVNEYKEAKALGVDTVPVLVGPVSYLLLSKPAKGVEKSFALLSL 180

Query: 246 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLNVLI 305
           +  ILPVYKEV++ELKAAGATWIQFDEPTLV DLD+H L AF+ AY+ELES LSGLNVLI
Sbjct: 181 LSSILPVYKEVIAELKAAGATWIQFDEPTLVLDLDSHQLAAFSAAYTELESALSGLNVLI 240

Query: 306 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIK-GGFPSGKYLFAGVVDGRNIW 365
           ETYFAD+PAE+YKTLTSL  V+ YGFDL+RG++TLDL+K  GFPSGKYLFAGVVDGRNIW
Sbjct: 241 ETYFADIPAESYKTLTSLNSVTAYGFDLIRGSKTLDLVKSAGFPSGKYLFAGVVDGRNIW 300

Query: 366 SNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVV 425
           ++DL AS+TTLE+LE +VGK+K+VVSTSCSL+HTAVDLVNETKLD EIKSWLAFAAQKVV
Sbjct: 301 ADDLAASLTTLESLEAIVGKDKLVVSTSCSLMHTAVDLVNETKLDSEIKSWLAFAAQKVV 360

Query: 426 EVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSAR 485
           EVNALAKAL+G KDEAYF++N AAQASR+SSPRVTNE VQKAAAAL+GSDHRR+TNVSAR
Sbjct: 361 EVNALAKALAGQKDEAYFAANTAAQASRRSSPRVTNEEVQKAAAALRGSDHRRATNVSAR 420

Query: 486 LDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQE 545
           LDAQQKKLNLP+LPTTTIGSFPQT++LRRVRRE+KAKKISE++YVS+IKEEI+KVV++QE
Sbjct: 421 LDAQQKKLNLPVLPTTTIGSFPQTVELRRVRREYKAKKISEDEYVSAIKEEISKVVKIQE 480

Query: 546 ELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMT 605
           ELDIDVLVHGEPERNDMVEYFGEQLSGFAFT NGWVQSYGSRCVKPPIIYGDVSRP  MT
Sbjct: 481 ELDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPNAMT 540

Query: 606 VFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGI 665
           VFWS MAQSMT RPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIK EVEDLE  GI
Sbjct: 541 VFWSKMAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKKEVEDLEAGGI 600

Query: 666 TVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHS 725
            VIQIDEAALREGLPLRK+E  FYL+WAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHS
Sbjct: 601 QVIQIDEAALREGLPLRKAEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHS 660

Query: 726 IIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKML 785
           II+MDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKML
Sbjct: 661 IINMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKML 720

Query: 786 AVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 831
           AVL++NILWVNPDCGLKTRKYTEVKPAL NMV AAKL+R+QLASAK
Sbjct: 721 AVLDTNILWVNPDCGLKTRKYTEVKPALTNMVLAAKLIRTQLASAK 766

BLAST of Cucsat.G7170.T1 vs. ExPASy Swiss-Prot
Match: P93263 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Mesembryanthemum crystallinum OX=3544 GN=METE PE=2 SV=1)

HSP 1 Score: 1341.6 bits (3471), Expect = 0.0e+00
Identity = 667/765 (87.19%), Postives = 720/765 (94.12%), Query Frame = 0

Query: 66  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 125
           MASHIVGYPR+GPKRELKFALESFWDGKS+A+DL+KV+ADLRSSIWKQMA+AGIK+IPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDGKSTAEDLKKVSADLRSSIWKQMADAGIKYIPSN 60

Query: 126 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 185
           TFSYYDQVLDT  MLGAVP RYGW GGEI FD+YFSMARGNASVPAMEMTKWFDTNYH+I
Sbjct: 61  TFSYYDQVLDTTAMLGAVPPRYGWTGGEIEFDVYFSMARGNASVPAMEMTKWFDTNYHFI 120

Query: 186 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 245
           VPEL PE  F+YASHKAV E+KEAKALGV+TVPVLVGPVS+LLLSK AKGV+KSF LLSL
Sbjct: 121 VPELGPEVNFSYASHKAVLEYKEAKALGVDTVPVLVGPVSYLLLSKQAKGVDKSFDLLSL 180

Query: 246 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLNVLI 305
           + KILP+YKEVV+ELK AGA+WIQFDEP LV DL++H LQAF+ AY++LESTLSGLNV++
Sbjct: 181 LPKILPIYKEVVAELKEAGASWIQFDEPLLVMDLESHKLQAFSAAYADLESTLSGLNVVV 240

Query: 306 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDGRNIWS 365
           ETYFADV AEAYKTL SLKGV+GYGFDLVRGT+TLDL+K  FPSGKYLFAGVVDGRNIW+
Sbjct: 241 ETYFADVTAEAYKTLISLKGVTGYGFDLVRGTKTLDLVKAEFPSGKYLFAGVVDGRNIWA 300

Query: 366 NDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVVE 425
           NDL AS+ TLEALE VVGK+K+VVSTSCS LHTAVDL+NETKLDDEIKSWLAFAAQKV+E
Sbjct: 301 NDLAASLATLEALEGVVGKDKLVVSTSCSFLHTAVDLINETKLDDEIKSWLAFAAQKVLE 360

Query: 426 VNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSARL 485
           VNALAKALSG KDEA+FS+NAAA ASRKSSPRVTNEAVQKAA ALKGSDHRR+T VS+RL
Sbjct: 361 VNALAKALSGQKDEAFFSANAAALASRKSSPRVTNEAVQKAATALKGSDHRRATTVSSRL 420

Query: 486 DAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQEE 545
           DAQQKKLNLP+LPTTTIGSFPQT++LRRVRRE+KAKKISEE+YV +IKEEI+KVV+LQEE
Sbjct: 421 DAQQKKLNLPILPTTTIGSFPQTVELRRVRREYKAKKISEEEYVKAIKEEISKVVKLQEE 480

Query: 546 LDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTV 605
           LDIDVLVHGEPERNDMVEYFGEQLSGFAF+ NGWVQSYGSRCVKPPIIYGDVSRP PMTV
Sbjct: 481 LDIDVLVHGEPERNDMVEYFGEQLSGFAFSANGWVQSYGSRCVKPPIIYGDVSRPNPMTV 540

Query: 606 FWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGIT 665
           FWS+MAQSMT RPMKGMLTGPVTILNWSFVRNDQPR ETCYQIALAIK+EVEDLEKAGI 
Sbjct: 541 FWSSMAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKNEVEDLEKAGIN 600

Query: 666 VIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSI 725
           VIQIDEAALREGLPLRKSE +FYL WAVHSFRITN GVQDTTQIHTHMCYSNFNDIIHSI
Sbjct: 601 VIQIDEAALREGLPLRKSEHDFYLKWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSI 660

Query: 726 IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLA 785
           IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIP TEE+ADRI KMLA
Sbjct: 661 IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPPTEELADRIRKMLA 720

Query: 786 VLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 831
           VLESN+LWVNPDCGLKTRKY EV PAL NMVAAAK LR +LASAK
Sbjct: 721 VLESNVLWVNPDCGLKTRKYGEVNPALSNMVAAAKQLRQELASAK 765

BLAST of Cucsat.G7170.T1 vs. NCBI nr
Match: XP_004144074.1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [Cucumis sativus] >KGN66367.1 hypothetical protein Csa_007688 [Cucumis sativus])

HSP 1 Score: 1600 bits (4142), Expect = 0.0
Identity = 824/830 (99.28%), Postives = 826/830 (99.52%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MTQLPLKSLEPFAISSSSSSS   FSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIR+TS
Sbjct: 1   MTQLPLKSLEPFAISSSSSSS---FSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRSTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK
Sbjct: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAF QAYSELESTLSG
Sbjct: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQT+DLIKGGFPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480
           QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480

Query: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540
           VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV
Sbjct: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540

Query: 541 QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP 600
           QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP
Sbjct: 541 QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP 600

Query: 601 KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE 660
           KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE
Sbjct: 601 KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE 660

Query: 661 KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND 720
           KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND
Sbjct: 661 KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND 720

Query: 721 IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRI 780
           IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRI
Sbjct: 721 IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRI 780

Query: 781 NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 830
           NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK
Sbjct: 781 NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 827

BLAST of Cucsat.G7170.T1 vs. NCBI nr
Match: XP_008451024.1 (PREDICTED: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [Cucumis melo])

HSP 1 Score: 1594 bits (4128), Expect = 0.0
Identity = 823/830 (99.16%), Postives = 826/830 (99.52%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MTQLPLKSLEPFAISSSSSSSSSS SIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS
Sbjct: 1   MTQLPLKSLEPFAISSSSSSSSSS-SIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMA+AGIK
Sbjct: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMADAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELESTLSG
Sbjct: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESTLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSN+LQASITTLEAL NVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNNLQASITTLEALGNVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480
           QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRR+TN
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATN 480

Query: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540
           VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV
Sbjct: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540

Query: 541 QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP 600
           QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP
Sbjct: 541 QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP 600

Query: 601 KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE 660
           KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE
Sbjct: 601 KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE 660

Query: 661 KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND 720
           KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND
Sbjct: 661 KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND 720

Query: 721 IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRI 780
           IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRI
Sbjct: 721 IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRI 780

Query: 781 NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 830
           NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK
Sbjct: 781 NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 829

BLAST of Cucsat.G7170.T1 vs. NCBI nr
Match: XP_038879499.1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 1583 bits (4100), Expect = 0.0
Identity = 814/830 (98.07%), Postives = 822/830 (99.04%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MTQLPLKSLEPFAISSSSSSSSS   IFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS
Sbjct: 1   MTQLPLKSLEPFAISSSSSSSSS---IFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMA+AGIK
Sbjct: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMADAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNE+KEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEYKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSL+DKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELES LSG
Sbjct: 241 SLLSLVDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESVLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSNDLQASITTLEALE VVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNDLQASITTLEALEKVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480
           QKVVEVNALAKAL+GHKDEAYFS+NAAAQASRKSSPRVTNEAVQKAAAALKGSDHRR+TN
Sbjct: 421 QKVVEVNALAKALAGHKDEAYFSANAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATN 480

Query: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540
           VS RLDAQQKKLNLP+LPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV
Sbjct: 481 VSVRLDAQQKKLNLPILPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540

Query: 541 QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP 600
           QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP
Sbjct: 541 QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP 600

Query: 601 KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE 660
           KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE
Sbjct: 601 KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE 660

Query: 661 KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND 720
           KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND
Sbjct: 661 KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND 720

Query: 721 IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRI 780
           IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPST+EIADRI
Sbjct: 721 IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTDEIADRI 780

Query: 781 NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 830
           NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK
Sbjct: 781 NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 827

BLAST of Cucsat.G7170.T1 vs. NCBI nr
Match: XP_023533330.1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1535 bits (3975), Expect = 0.0
Identity = 788/830 (94.94%), Postives = 804/830 (96.87%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MT LPLKSL+ F ISS SSSS+S     ALRRG IAPFSYLL SSIH LPSLRLSIRTTS
Sbjct: 1   MTLLPLKSLDSFGISSCSSSSTS----IALRRGTIAPFSYLLVSSIHRLPSLRLSIRTTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASH+VGYPRIGPKRELKFALESFWDGKS+ADDLQKVA DLR+SIWKQMA+AGIK
Sbjct: 61  TVVRAMASHVVGYPRIGPKRELKFALESFWDGKSTADDLQKVATDLRASIWKQMADAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFD YFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDTYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNE+KEAKALGVET+PVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEYKEAKALGVETIPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSL+DKILP+YKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELES LSG
Sbjct: 241 SLLSLVDKILPIYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESVLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKG FPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGEFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSNDLQAS+ TLEAL+NVVGKE IVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNDLQASLNTLEALQNVVGKENIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480
           QKVVEVNALAKALSGHKDEAYFS+NAAAQASRKSSPRVTNEAVQKAAAALK SDHRR+TN
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSANAAAQASRKSSPRVTNEAVQKAAAALKSSDHRRATN 480

Query: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540
           VS RLDAQQKKLNLP LPTTTIGSFPQTMDLRRVRREFKAKKISEE YVS+IKEEINKVV
Sbjct: 481 VSVRLDAQQKKLNLPKLPTTTIGSFPQTMDLRRVRREFKAKKISEEVYVSNIKEEINKVV 540

Query: 541 QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP 600
           QLQEEL IDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP
Sbjct: 541 QLQEELGIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP 600

Query: 601 KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE 660
           KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE
Sbjct: 601 KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE 660

Query: 661 KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND 720
           KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND
Sbjct: 661 KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND 720

Query: 721 IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRI 780
           IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPST+EIADRI
Sbjct: 721 IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTDEIADRI 780

Query: 781 NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 830
           NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMV AAKLLRSQLASAK
Sbjct: 781 NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVDAAKLLRSQLASAK 826

BLAST of Cucsat.G7170.T1 vs. NCBI nr
Match: XP_022147614.1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like [Momordica charantia])

HSP 1 Score: 1534 bits (3972), Expect = 0.0
Identity = 785/828 (94.81%), Postives = 804/828 (97.10%), Query Frame = 0

Query: 3   QLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTSTV 62
           QL LKS EPFA SS+SS       I ALRRGAI+PF YLLFSSIHHLPS RLSIRTTSTV
Sbjct: 5   QLSLKSHEPFAKSSASS-------IIALRRGAISPFPYLLFSSIHHLPSRRLSIRTTSTV 64

Query: 63  VRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFI 122
           +RAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMA+AGIKFI
Sbjct: 65  IRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMADAGIKFI 124

Query: 123 PSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNY 182
           PSNTFSYYDQVLDTATMLGAVP RYGWNGGEIGFD YFSMARGNASVPAMEMTKWFDTNY
Sbjct: 125 PSNTFSYYDQVLDTATMLGAVPPRYGWNGGEIGFDTYFSMARGNASVPAMEMTKWFDTNY 184

Query: 183 HYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSL 242
           HYIVPELSP+DKF YASHKAVNE+KEAKALGV+T+PVLVGPVSFL+LSKPAKGVEKSFSL
Sbjct: 185 HYIVPELSPDDKFIYASHKAVNEYKEAKALGVDTIPVLVGPVSFLVLSKPAKGVEKSFSL 244

Query: 243 LSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLN 302
           LSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELES LSGLN
Sbjct: 245 LSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESVLSGLN 304

Query: 303 VLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDGRN 362
           VLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQT DLIK GFPSGKYLFAGVVDGRN
Sbjct: 305 VLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTFDLIKSGFPSGKYLFAGVVDGRN 364

Query: 363 IWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQK 422
           IWSNDLQAS+ TL+ALEN VGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQK
Sbjct: 365 IWSNDLQASLNTLQALENAVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQK 424

Query: 423 VVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVS 482
           V+EVNALAKALSG KDEAYFS+NAAAQASRKSSPRVTNEAVQKAAAALKGSDHRR+TNVS
Sbjct: 425 VIEVNALAKALSGKKDEAYFSTNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATNVS 484

Query: 483 ARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQL 542
           ARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEE+YVSSIKEEINKVVQL
Sbjct: 485 ARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEENYVSSIKEEINKVVQL 544

Query: 543 QEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKP 602
           QEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKP
Sbjct: 545 QEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKP 604

Query: 603 MTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKA 662
           MTVFWS+MAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKA
Sbjct: 605 MTVFWSSMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKA 664

Query: 663 GITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDII 722
           GITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDII
Sbjct: 665 GITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDII 724

Query: 723 HSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINK 782
           HSIIDMDADVITIENSRSDEK+LSVFREGVKYGAGIGPGVYDIHSPRIPST+EIADRINK
Sbjct: 725 HSIIDMDADVITIENSRSDEKILSVFREGVKYGAGIGPGVYDIHSPRIPSTDEIADRINK 784

Query: 783 MLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 830
           MLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLA+AK
Sbjct: 785 MLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLANAK 825

BLAST of Cucsat.G7170.T1 vs. ExPASy TrEMBL
Match: A0A0A0LZR2 (5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_1G599580 PE=3 SV=1)

HSP 1 Score: 1600 bits (4142), Expect = 0.0
Identity = 824/830 (99.28%), Postives = 826/830 (99.52%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MTQLPLKSLEPFAISSSSSSS   FSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIR+TS
Sbjct: 1   MTQLPLKSLEPFAISSSSSSS---FSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRSTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK
Sbjct: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAF QAYSELESTLSG
Sbjct: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQT+DLIKGGFPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480
           QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480

Query: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540
           VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV
Sbjct: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540

Query: 541 QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP 600
           QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP
Sbjct: 541 QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP 600

Query: 601 KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE 660
           KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE
Sbjct: 601 KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE 660

Query: 661 KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND 720
           KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND
Sbjct: 661 KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND 720

Query: 721 IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRI 780
           IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRI
Sbjct: 721 IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRI 780

Query: 781 NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 830
           NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK
Sbjct: 781 NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 827

BLAST of Cucsat.G7170.T1 vs. ExPASy TrEMBL
Match: A0A1S3BQJ8 (5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103492431 PE=3 SV=1)

HSP 1 Score: 1594 bits (4128), Expect = 0.0
Identity = 823/830 (99.16%), Postives = 826/830 (99.52%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MTQLPLKSLEPFAISSSSSSSSSS SIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS
Sbjct: 1   MTQLPLKSLEPFAISSSSSSSSSS-SIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMA+AGIK
Sbjct: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMADAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELESTLSG
Sbjct: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESTLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSN+LQASITTLEAL NVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNNLQASITTLEALGNVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480
           QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRR+TN
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATN 480

Query: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540
           VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV
Sbjct: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540

Query: 541 QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP 600
           QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP
Sbjct: 541 QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP 600

Query: 601 KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE 660
           KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE
Sbjct: 601 KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE 660

Query: 661 KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND 720
           KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND
Sbjct: 661 KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND 720

Query: 721 IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRI 780
           IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRI
Sbjct: 721 IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRI 780

Query: 781 NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 830
           NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK
Sbjct: 781 NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 829

BLAST of Cucsat.G7170.T1 vs. ExPASy TrEMBL
Match: A0A6J1D1J0 (5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111016496 PE=3 SV=1)

HSP 1 Score: 1534 bits (3972), Expect = 0.0
Identity = 785/828 (94.81%), Postives = 804/828 (97.10%), Query Frame = 0

Query: 3   QLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTSTV 62
           QL LKS EPFA SS+SS       I ALRRGAI+PF YLLFSSIHHLPS RLSIRTTSTV
Sbjct: 5   QLSLKSHEPFAKSSASS-------IIALRRGAISPFPYLLFSSIHHLPSRRLSIRTTSTV 64

Query: 63  VRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFI 122
           +RAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMA+AGIKFI
Sbjct: 65  IRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMADAGIKFI 124

Query: 123 PSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNY 182
           PSNTFSYYDQVLDTATMLGAVP RYGWNGGEIGFD YFSMARGNASVPAMEMTKWFDTNY
Sbjct: 125 PSNTFSYYDQVLDTATMLGAVPPRYGWNGGEIGFDTYFSMARGNASVPAMEMTKWFDTNY 184

Query: 183 HYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSL 242
           HYIVPELSP+DKF YASHKAVNE+KEAKALGV+T+PVLVGPVSFL+LSKPAKGVEKSFSL
Sbjct: 185 HYIVPELSPDDKFIYASHKAVNEYKEAKALGVDTIPVLVGPVSFLVLSKPAKGVEKSFSL 244

Query: 243 LSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLN 302
           LSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELES LSGLN
Sbjct: 245 LSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESVLSGLN 304

Query: 303 VLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDGRN 362
           VLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQT DLIK GFPSGKYLFAGVVDGRN
Sbjct: 305 VLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTFDLIKSGFPSGKYLFAGVVDGRN 364

Query: 363 IWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQK 422
           IWSNDLQAS+ TL+ALEN VGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQK
Sbjct: 365 IWSNDLQASLNTLQALENAVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQK 424

Query: 423 VVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVS 482
           V+EVNALAKALSG KDEAYFS+NAAAQASRKSSPRVTNEAVQKAAAALKGSDHRR+TNVS
Sbjct: 425 VIEVNALAKALSGKKDEAYFSTNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATNVS 484

Query: 483 ARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQL 542
           ARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEE+YVSSIKEEINKVVQL
Sbjct: 485 ARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEENYVSSIKEEINKVVQL 544

Query: 543 QEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKP 602
           QEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKP
Sbjct: 545 QEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKP 604

Query: 603 MTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKA 662
           MTVFWS+MAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKA
Sbjct: 605 MTVFWSSMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKA 664

Query: 663 GITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDII 722
           GITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDII
Sbjct: 665 GITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDII 724

Query: 723 HSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINK 782
           HSIIDMDADVITIENSRSDEK+LSVFREGVKYGAGIGPGVYDIHSPRIPST+EIADRINK
Sbjct: 725 HSIIDMDADVITIENSRSDEKILSVFREGVKYGAGIGPGVYDIHSPRIPSTDEIADRINK 784

Query: 783 MLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 830
           MLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLA+AK
Sbjct: 785 MLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLANAK 825

BLAST of Cucsat.G7170.T1 vs. ExPASy TrEMBL
Match: A0A6J1JBY9 (5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111485391 PE=3 SV=1)

HSP 1 Score: 1528 bits (3955), Expect = 0.0
Identity = 784/830 (94.46%), Postives = 800/830 (96.39%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MT LP+KSL+ F IS  SSSS+S     ALRRG IAPFSYLL SSIH LPSLRLSIRT S
Sbjct: 1   MTLLPVKSLDSFGISPCSSSSTS----IALRRGTIAPFSYLLVSSIHRLPSLRLSIRTAS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASH+VGYPRIGPKRELKFALESFWDGKS+ADDLQKVA DLR+SIWKQMA+AGIK
Sbjct: 61  TVVRAMASHVVGYPRIGPKRELKFALESFWDGKSTADDLQKVATDLRASIWKQMADAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFD YFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDTYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNE+KEAKALGVET+PVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEYKEAKALGVETIPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSL+ KILP+YKEVVSELKAAGATWIQFDEPTLVKDLD H LQAFA AYSELES LSG
Sbjct: 241 SLLSLVGKILPIYKEVVSELKAAGATWIQFDEPTLVKDLDTHQLQAFAHAYSELESVLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKG FPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGKFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSNDLQAS+ TLEAL+NVVGKE IVVSTSCSLLHTAVDLVNETKLDDEIK WLAFAA
Sbjct: 361 RNIWSNDLQASLNTLEALQNVVGKENIVVSTSCSLLHTAVDLVNETKLDDEIKLWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480
           QKVVEVNALAKALSGHKDEAYFS+NAAAQASRKSSPRVTNEAVQKAAAALK SDHRR+TN
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSANAAAQASRKSSPRVTNEAVQKAAAALKSSDHRRATN 480

Query: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540
           VS RLDAQQKKLNLP LPTTTIGSFPQTMDLRRVRREFKAKKISEE YVSSIKEEINKVV
Sbjct: 481 VSVRLDAQQKKLNLPKLPTTTIGSFPQTMDLRRVRREFKAKKISEEVYVSSIKEEINKVV 540

Query: 541 QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP 600
           QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP
Sbjct: 541 QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP 600

Query: 601 KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE 660
           KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE
Sbjct: 601 KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE 660

Query: 661 KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND 720
           KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND
Sbjct: 661 KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND 720

Query: 721 IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRI 780
           IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPST+EIADRI
Sbjct: 721 IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTDEIADRI 780

Query: 781 NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 830
           NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMV AAKLLRSQLASAK
Sbjct: 781 NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVDAAKLLRSQLASAK 826

BLAST of Cucsat.G7170.T1 vs. ExPASy TrEMBL
Match: A0A6J1HAA9 (5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111461556 PE=3 SV=1)

HSP 1 Score: 1528 bits (3955), Expect = 0.0
Identity = 784/830 (94.46%), Postives = 800/830 (96.39%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MT LPLKSL+ F ISS SSSS+S     ALRRG I PFSYLL SSIH  PSLRLSIRTTS
Sbjct: 1   MTLLPLKSLDSFGISSCSSSSTS----IALRRGTITPFSYLLVSSIHRRPSLRLSIRTTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASH+VGYPRIGPKRELKFALESFWDGKS+ADDLQKVA DLR+SIWKQMA+AGIK
Sbjct: 61  TVVRAMASHVVGYPRIGPKRELKFALESFWDGKSTADDLQKVATDLRASIWKQMADAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFD YFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDTYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNE+KEAKALGVET+PVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEYKEAKALGVETIPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSL+DKILP+YKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELES LSG
Sbjct: 241 SLLSLVDKILPIYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESVLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKG FPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGEFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSNDLQAS+ TLEAL+NVVGKE IVVSTSCSLLHTAVDL NETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNDLQASLNTLEALQNVVGKENIVVSTSCSLLHTAVDLANETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTN 480
           QKVVEVNALAKALSGHKDEAYFS+NAAAQASRKSSPRVTNEAVQKAAAALK SDHRR+TN
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSANAAAQASRKSSPRVTNEAVQKAAAALKSSDHRRATN 480

Query: 481 VSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVV 540
           VS RLDAQQKKL LP LPTTTIGSFPQTMDLRRVRREFKAKKISEE YVS+IKEEINKVV
Sbjct: 481 VSVRLDAQQKKLKLPKLPTTTIGSFPQTMDLRRVRREFKAKKISEEVYVSNIKEEINKVV 540

Query: 541 QLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP 600
           QLQEEL IDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP
Sbjct: 541 QLQEELGIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRP 600

Query: 601 KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE 660
           KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE
Sbjct: 601 KPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLE 660

Query: 661 KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND 720
           KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND
Sbjct: 661 KAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFND 720

Query: 721 IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRI 780
           IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPST+EIADRI
Sbjct: 721 IIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTDEIADRI 780

Query: 781 NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 830
           NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMV AAKLLRSQLASAK
Sbjct: 781 NKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVDAAKLLRSQLASAK 826

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q426990.0e+0088.105-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Cathara... [more]
Q2QLY50.0e+0087.215-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 OS=Oryza... [more]
O500080.0e+0087.325-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 OS=Arabi... [more]
Q2QLY40.0e+0086.955-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2 OS=Oryza... [more]
P932630.0e+0087.195-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Mesembr... [more]
Match NameE-valueIdentityDescription
XP_004144074.10.099.285-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [Cucumis s... [more]
XP_008451024.10.099.16PREDICTED: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase... [more]
XP_038879499.10.098.075-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1-like [Be... [more]
XP_023533330.10.094.945-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1-like [Cu... [more]
XP_022147614.10.094.815-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase-like [Momo... [more]
Match NameE-valueIdentityDescription
A0A0A0LZR20.099.285-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucum... [more]
A0A1S3BQJ80.099.165-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucum... [more]
A0A6J1D1J00.094.815-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Momor... [more]
A0A6J1JBY90.094.465-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucur... [more]
A0A6J1HAA90.094.465-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucur... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013215Cobalamin-independent methionine synthase MetE, N-terminalPFAMPF08267Meth_synt_1coord: 68..381
e-value: 2.5E-120
score: 401.7
IPR038071UROD/MetE-like superfamilyGENE3D3.20.20.210coord: 70..590
e-value: 0.0
score: 1157.5
IPR038071UROD/MetE-like superfamilyGENE3D3.20.20.210coord: 455..823
e-value: 0.0
score: 1157.5
IPR038071UROD/MetE-like superfamilySUPERFAMILY51726UROD/MetE-likecoord: 68..460
IPR038071UROD/MetE-like superfamilySUPERFAMILY51726UROD/MetE-likecoord: 462..824
IPR006276Cobalamin-independent methionine synthaseTIGRFAMTIGR01371TIGR01371coord: 71..825
e-value: 0.0
score: 1152.2
IPR006276Cobalamin-independent methionine synthaseHAMAPMF_00172Meth_synthcoord: 66..825
score: 34.788776
IPR002629Cobalamin-independent methionine synthase MetE, C-terminal/archaealPFAMPF01717Meth_synt_2coord: 497..820
e-value: 4.1E-158
score: 525.8
IPR002629Cobalamin-independent methionine synthase MetE, C-terminal/archaealCDDcd03311CIMS_C_terminal_likecoord: 498..821
e-value: 1.64363E-147
score: 435.118
NoneNo IPR availablePANTHERPTHR30519:SF175-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE METHYLTRANSFERASE 3, CHLOROPLASTICcoord: 38..830
NoneNo IPR availablePANTHERPTHR305195-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE METHYLTRANSFERASEcoord: 38..830
NoneNo IPR availableCDDcd03312CIMS_N_terminal_likecoord: 68..432
e-value: 0.0
score: 599.903

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G7170Cucsat.G7170gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G7170.T1.E1Cucsat.G7170.T1.E1exon
Cucsat.G7170.T1.E2Cucsat.G7170.T1.E2exon
Cucsat.G7170.T1.E3Cucsat.G7170.T1.E3exon
Cucsat.G7170.T1.E4Cucsat.G7170.T1.E4exon
Cucsat.G7170.T1.E5Cucsat.G7170.T1.E5exon
Cucsat.G7170.T1.E6Cucsat.G7170.T1.E6exon
Cucsat.G7170.T1.E7Cucsat.G7170.T1.E7exon
Cucsat.G7170.T1.E8Cucsat.G7170.T1.E8exon
Cucsat.G7170.T1.E9Cucsat.G7170.T1.E9exon
Cucsat.G7170.T1.E10Cucsat.G7170.T1.E10exon
Cucsat.G7170.T1.E11Cucsat.G7170.T1.E11exon
Cucsat.G7170.T1.E12Cucsat.G7170.T1.E12exon
Cucsat.G7170.T1.E13Cucsat.G7170.T1.E13exon
Cucsat.G7170.T1.E14Cucsat.G7170.T1.E14exon
Cucsat.G7170.T1.E15Cucsat.G7170.T1.E15exon
Cucsat.G7170.T1.E16Cucsat.G7170.T1.E16exon
Cucsat.G7170.T1.E17Cucsat.G7170.T1.E17exon
Cucsat.G7170.T1.E18Cucsat.G7170.T1.E18exon
Cucsat.G7170.T1.E19Cucsat.G7170.T1.E19exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G7170.T1.C12Cucsat.G7170.T1.C12CDS
Cucsat.G7170.T1.C11Cucsat.G7170.T1.C11CDS
Cucsat.G7170.T1.C10Cucsat.G7170.T1.C10CDS
Cucsat.G7170.T1.C9Cucsat.G7170.T1.C9CDS
Cucsat.G7170.T1.C8Cucsat.G7170.T1.C8CDS
Cucsat.G7170.T1.C7Cucsat.G7170.T1.C7CDS
Cucsat.G7170.T1.C6Cucsat.G7170.T1.C6CDS
Cucsat.G7170.T1.C5Cucsat.G7170.T1.C5CDS
Cucsat.G7170.T1.C4Cucsat.G7170.T1.C4CDS
Cucsat.G7170.T1.C3Cucsat.G7170.T1.C3CDS
Cucsat.G7170.T1.C2Cucsat.G7170.T1.C2CDS
Cucsat.G7170.T1.C1Cucsat.G7170.T1.C1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G7170.T1Cucsat.G7170.T1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008652 cellular amino acid biosynthetic process
biological_process GO:0009086 methionine biosynthetic process
biological_process GO:0032259 methylation
cellular_component GO:0009507 chloroplast
cellular_component GO:0005829 cytosol
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003871 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0004045 aminoacyl-tRNA hydrolase activity
molecular_function GO:0008705 methionine synthase activity