Cucsat.G7170.T25 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G7170.T25
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
Locationctg1528: 2816373 .. 2822430 (-)
RNA-Seq ExpressionCucsat.G7170.T25
SyntenyCucsat.G7170.T25
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACAAAAAGTTTTCTTTTTCATAGTCTACCAATGACATTCTTAATAATATCAATTTTTTTTAGCTAAACATTTGTTATTACTGAAACGAAGTTAGAGCTAATGGCTGTAAAAGAAAGAAGGAAGAACATAAGGATGGCTTGGCACAAACGACCGTAATAACCCGGCCTTGATTTTGGATCTTGCGGGTGGGTTTGGTGGTTGATGTGAGAGAGAGAGAAAAAAATGAGGATCTCATATCAAAAAATGATGGGTTCGTTTTTGGTGGAGATGTGAGAGAATGAGAAAAATCCGAGTATAAATGACCCAACTTCCACTGAAATCGCTTGAGCCCTTTGCAATATCATCGTCTTCTTCTTCTTCTTCTTCTTCATTCTCTATTTTTGCACTGAGAAGAGGAGCGATTGCCCCATTTTCATATCTCCTTTTCTCCTCTATCCATCATCTTCCTTCCCTTCGCTTATCAATTCGCACTACTTCCACTGTTGTCAGGTATTTCTTTCACTCTCTGGTGCATTTCTGTGTTGTGGAGTCTTTTGGGGCTGTTTTTTGTATCGTTTTGTGATCTGGGTATGGATTATGTTAGCTATTTTTGTGGTTGATTGTTTGGAATTTACTTTATATCCTCTAATGGAAATGTGGGGGGTTTTTGTGAAATGGTCTTAGCTGATTGGTAATAGATGAACATGAAGGGCTCTGACTTTTTGTTTCGTTTTCTTTATGTTATTCCTTAGAGGCTTTGAGTTCAAGTAGGTCTTTAGTTTCTTTTACTTTTTTTTCTCTGTTGTTCTTTGATTTGGCTAAACATGATTAAGCGGCTGACGTTTCTCTTTTCTTCTTCTTTTTGCTTTAATGCCGTTTTGGTTGTTCTGTTATGGCATTTTTGTACTGATACAAAGTCCATAAGATTCGAACTAATGAGGTTTTTCATTTAGATATTTATGATTGGTTCTTCATATTAAAATTCTACATGTTGATTGCTGATTTTGTGAATTACGTGTGCCATTTTTTGAAATGAAATGAAACCGTTAATGTTTTTTCAGAAAATTTAGTTCGCATTAACATTTAATGGTGTGTCTATTTTGCTAATGGTATTCTTTCATGTATGCATAAAATGAAAAATTGTGTTCTTTTTAACGGTTTATACCATTACGTATGGCAAACAAGTGAAGTGATTCTGTTAACAACATAGTAACTTAAAATCATAATCACCATGGGTTGTCACAGTGGTCATTCTAAGGGTTGGTAAATAGAGGTTTTATAGAGAATGAGTTTGGTTTTATAGAGAATGAGTTCAAACCATGTTGCTACCTATTTAAGATTTAATATTTTACGAGTTACCTCGAGAGCTTAGTATGTAGCATGAGAGTAATCGAGGTGTCCACAAGGTAGTATAAAAACTTAATTTTTTTATCGTCTCCTTAAGTTGGCCTTGGTTGGGAAAAGCCTCCTAGAAATTAGTGAGGTGGATTGTGAACAAGGAATAGGTTTTCAATACCTCCATTAGCAAAAGAAAACTTATCTTATATTAGAGGAATATGGGAAATTGTTAATGAAAATGTTCATTGAGTTCTACTGACATTTCATTTTTATATTATTTTCTTGTTAATATGTAAAAGAGCAATGGCATCCCACATTGTTGGTTATCCTCGCATTGGTCCGAAGAGAGAACTTAAATTTGCCTTGGAGTCTTTCTGGGATGGGAAGAGCAGTGCTGATGACTTGCAGAAAGTGGCTGCTGATCTCAGGTCATCTATTTGGAAGCAGATGGCTGAAGCTGGAATCAAGTTTATTCCAAGCAACACTTTTTCGTATTATGATCAAGTGCTTGACACTGCAACTATGCTCGGAGCTGTTCCGTCAAGATATGGATGGAACGGTGGTGAGATTGGATTTGACATCTACTTCTCCATGGCTAGAGGGAACGCTTCAGTTCCTGCCATGGAAATGACGAAGTGGTTCGATACCAATTAGTAAGTGAAAAACTCTATGCATTTCCCTACATTTTTATGATAATACTATTTGTAAAACTTCTACTAGTCAATGATTTGAGGAATGTTAACGATGTGTGTTCTGTTACAGCCATTACATTGTGCCCGAGCTGAGTCCAGAAGATAAATTTACTTATGCATCTCACAAAGCAGTAAACGAGTTCAAGGAAGCTAAAGCTGTAAGATTTCTTATTAAAAGAAATTAGGGAAAAAAAAAAAAAACACTTAAAAATGAACTTATTTTCTGAGTTTGGATAATGGTGGTAGATTCAAGTTTCATGTGAATAAGATTAATTCAAGGAAGGATGTACTTAACTGGTTTGCACTTGTAATTTTTTTTTAATGTCCACCAAGAAAAAGTTTAAGAATTTTCTTGCCCTATGAACAGTAGTACAAGTTAACTTGAATCATCTATCCTTGAACTTGACAACAAACATCCTTTCCATTTCCAGTTTGATTAATTTTTTGCTTTCTTTGTCGTCTGTTAGTTCTTCTGATTCAGTGTTTTAATTTATTATTTCAATTTTTTTTTTATCATTTGACCTGCCCTACTGATGCATTTAACTTCTTTTATACTCCATCTTATATGATATTATATTTCTTATATTTAAAGAGGCATCTTGCTTTTATCATGCGACAGAACCTTATTTTTTAGCCATTCATAATAACAAGCAAGATGCCTTATTCGGACTGACATGCCTTTCAATTTAAGAATTGTTTCAAGTGAAGAATATTTGAATAAATGCCTGGATAGTAAATTATATTCTATGAGAATTTTATGATATTTGAAGTTCAGTCATGAAATTTCAATATGAGCATGTTAAGCATTTTTATTACCTTTTTTCCTTTACACTCCCACTTTTCTGTGCAGCTCGGAGTTGAGACTGTCCCGGTTCTTGTAGGCCCTGTATCCTTCTTGTTGCTTTCAAAACCAGCCAAGGGAGTTGAGAAATCCTTCTCACTTCTCTCCCTAATCGACAAGATTCTTCCTGTTTATAAGTCAGTATTTCTTTTCCTCTTTCCAAAGTTCGTCTAGTTTACGTATTAACCATTTCATTTCAGTTCCACTATCTTCTTATCAACTCTTATTTGTACCTGCAGGGAAGTTGTTTCAGAGCTGAAGGCTGCTGGTGCCACTTGGATTCAATTTGATGAGCCTACACTTGTGAAAGATCTTGACGCGCACCACTTACAAGCATTTGCTCAAGCCTACTCAGAGCTGGAATCCACTTTATCTGGTTTAAATGTTCTGATTGAGACATATTTTGCTGATGTTCCTGCTGAGGCATACAAAACTCTCACGTCTTTGAAGGGTGTCTCTGGTTATGGATTCGATCTAGTTCGTGGAACACAGACACTTGATCTGATCAAAGGTGGATTTCCTTCAGGCAAATATCTCTTTGCCGGAGTGGTTGATGGAAGAAACATATGGTCAAATGACCTTCAAGCTTCCATAACTACCTTGGAGGCGTTGGAAAACGTTGTTGGAAAAGGTGCTTGTTTCTTAACGTGCTAGCACATTTTATATTAGCGTGGATACATTTTTAGACTATAAACAGTGGTAATATATGCTGTTCTTCTGTCCTTTACAGAAAAAATTGTCGTCTCCACTTCCTGCTCTCTTCTTCACACTGCTGTTGATCTGGTGAACGAAACAAAACTAGATGATGAAATTAAGTCCTGGCTTGCTTTTGCCGCTCAGAAAGTTGTTGAAGTTAATGCCTTGGCTAAGGCACTCTCTGGACATAAAGATGAGGTACACGAAGCAACCTTATTTTTGACCTTGCAATAGGAACTTAGGGGCAGGGTGTTGTACCTTAAATCAAACAATGTAGCCTTAGTGCATAGTCTTTGTAGACTCATGGTACAAACGAAACATGTGTGTGTGTATATAATAAAATAAATGAAGCTTGACTTATTTGACATGAAATGGAAATCTATTGTTGCGGAACTGAAGATACTAAAATTTGAGCTTATAACTTTTTTCCTTTTAGGCAAAGAGAAAAATGAGCTTCTAACTGTCGCTTCTTCTATTCCAGGCATATTTTTCTTCCAATGCTGCTGCTCAAGCTTCAAGGAAATCATCCCCAAGAGTGACAAATGAGGCAGTCCAAAAAGCTGTATAAATTATTTGATGTACTAATATCATTTTGTAGGATCTCTCTATATTCTTGCATATCGTGTGCTTAACCTTCTATGTCGACTTTACTTAGGCTGCTGCTCTGAAGGGCTCTGACCACCGTCGGTCAACAAATGTAAGTGCCAGGCTGGACGCTCAGCAGAAGAAGTTGAACCTTCCAATGCTGCCTACAACAACAATTGGATCCTTCCCTCAAACTATGGATCTTAGAAGAGTGCGACGTGAATTCAAAGCAAAAAAGTATGCCTTTTTGTCTTCAGATATAATTGTTCGTCCTCAAAAAAGTACATTGTTGTTAATTTTCTAATACACTTGCATCCTGTTCCTTTAGAATCTCTGAGGAAGACTATGTCAGCTCCATCAAGGAGGAAATCAACAAAGTTGTTCAGCTCCAGGAAGAGCTTGACATCGATGTTTTGGTGCATGGAGAGCCTGAGGTAAGCATTTGTCTTCAAACAATGTTGAAGAAATTTAACTAACCATACATTGTCTAAGTTTAAACTGTAGCATCTGAATGTTAACGTCTCAGAGAAATGATATGGTTGAGTACTTTGGAGAGCAACTGTCTGGCTTTGCCTTCACTGTCAATGGTTGGGTCCAATCCTATGGGTCCCGTTGTGTCAAGCCGCCTATCATCTATGGTGATGTCAGTCGTCCAAAACCCATGACCGTCTTTTGGTCGACAATGGCTCAGAGCATGACGAAGCGGCCTATGAAGGGAATGCTTACTGGTCCAGTTACAATTTTGAACTGGTCTTTTGTTCGAAATGACCAACCAAGGTATGCGAATTTAATGAATCTCAGTTGCTGTTTCTTTGATCGTCTGTTTTATTGAGGGCAGTTCATGGTAGTTAATTCAATTGTTTTTCATAGGTTTGAAACATGCTACCAAATTGCCTTGGCAATCAAGGACGAGGTTGAGGATCTTGAGAAAGCTGGTATTACAGTCATCCAGATTGATGAAGCAGCACTGCGAGAAGGTTTGCCTCTCCGAAAGTCCGAGGAAAATTTCTATTTGAATTGGGCTGTTCATTCATTTCGAATCACCAATTGCGGTGTTCAAGATACCACCCAGGTCCTTTCCTACTCTCTTTTGCTTAAAAGTCCGATTATTTCTCTCACTAAATCAATTTGTTAGCGTTGTTTCATGTCATTTGCATTGAGTCGTATAACCGTCTTTTCAATGACATAGTGAACTTTATGATTTTGATGACTGCAGATCCACACCCACATGTGCTACTCGAACTTCAACGACATCATCCATTCGATAATCGACATGGATGCTGATGTGATCACCATTGAGAATTCCAGATCAGATGAGAAGCTCCTGTCTGTCTTCCGTGAGGGAGTAAAATACGGTGCTGGAATTGGACCAGGAGTCTACGACATCCATTCTCCCCGTATTCCCTCTACTGAGGAAATTGCTGACAGGATCAACAAGATGCTCGCTGTTCTTGAAAGCAACATCCTCTGGGTGAACCCTGACTGCGGTCTTAAAACCCGAAAGTACACTGAAGTCAAGCCTGCCCTCCTCAACATGGTTGCTGCTGCTAAGCTCCTTCGCTCCCAATTGGCCAGTGCCAAGTGAGTTTGAGAATGCTGTGGCAAACCTTAGGAAGAAAAACACTTAAAATTTTTCAATAAGATTTTGGCCTTCTGGTGAAGTGATCTTGTTGTTCTCTTTCTTGGAGTGTCTTCAATTTCTTAGGGAAGTAATGAACAATGTCGTCAAATTCCCATTGTGGCTTTTGTTTAAAAAGTTCAAAATTTTCTGTTTTTTTAATCAGTTTTTCTCGTTCAAGCCATTTTTTTTTGGTTTGTTTTGTAAAATAGTGGAGATGAGAATTAATGATATTAATAGATATACTTGTGTTTTGTCACACTACTTTGGGATAAAAGTAAAAGCAACAATTTAAAAGTATATTTTG

Coding sequence (CDS)

ATGACCCAACTTCCACTGAAATCGCTTGAGCCCTTTGCAATATCATCGTCTTCTTCTTCTTCTTCTTCTTCATTCTCTATTTTTGCACTGAGAAGAGGAGCGATTGCCCCATTTTCATATCTCCTTTTCTCCTCTATCCATCATCTTCCTTCCCTTCGCTTATCAATTCGCACTACTTCCACTGTTGTCAGAGCAATGGCATCCCACATTGTTGGTTATCCTCGCATTGGTCCGAAGAGAGAACTTAAATTTGCCTTGGAGTCTTTCTGGGATGGGAAGAGCAGTGCTGATGACTTGCAGAAAGTGGCTGCTGATCTCAGGTCATCTATTTGGAAGCAGATGGCTGAAGCTGGAATCAAGTTTATTCCAAGCAACACTTTTTCGTATTATGATCAAGTGCTTGACACTGCAACTATGCTCGGAGCTGTTCCGTCAAGATATGGATGGAACGGTGGTGAGATTGGATTTGACATCTACTTCTCCATGGCTAGAGGGAACGCTTCAGTTCCTGCCATGGAAATGACGAAGTGGTTCGATACCAATTACCATTACATTGTGCCCGAGCTGAGTCCAGAAGATAAATTTACTTATGCATCTCACAAAGCAGTAAACGAGTTCAAGGAAGCTAAAGCTCTCGGAGTTGAGACTGTCCCGGTTCTTGTAGGCCCTGTATCCTTCTTGTTGCTTTCAAAACCAGCCAAGGGAGTTGAGAAATCCTTCTCACTTCTCTCCCTAATCGACAAGATTCTTCCTGTTTATAAGGAAGTTGTTTCAGAGCTGAAGGCTGCTGGTGCCACTTGGATTCAATTTGATGAGCCTACACTTGTGAAAGATCTTGACGCGCACCACTTACAAGCATTTGCTCAAGCCTACTCAGAGCTGGAATCCACTTTATCTGGTTTAAATGTTCTGATTGAGACATATTTTGCTGATGTTCCTGCTGAGGCATACAAAACTCTCACGTCTTTGAAGGGTGTCTCTGGTTATGGATTCGATCTAGTTCGTGGAACACAGACACTTGATCTGATCAAAGGTGGATTTCCTTCAGGCAAATATCTCTTTGCCGGAGTGGTTGATGGAAGAAACATATGGTCAAATGACCTTCAAGCTTCCATAACTACCTTGGAGGCGTTGGAAAACGTTGTTGGAAAAGAAAAAATTGTCGTCTCCACTTCCTGCTCTCTTCTTCACACTGCTGTTGATCTGGTGAACGAAACAAAACTAGATGATGAAATTAAGTCCTGGCTTGCTTTTGCCGCTCAGAAAGTTGTTGAAGTTAATGCCTTGGCTAAGGCACTCTCTGGACATAAAGATGAGGCATATTTTTCTTCCAATGCTGCTGCTCAAGCTTCAAGGAAATCATCCCCAAGAGTGACAAATGAGGCAGTCCAAAAAGCTGTATAA

Protein sequence

MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTSTVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDGRNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAV
Homology
BLAST of Cucsat.G7170.T25 vs. ExPASy Swiss-Prot
Match: Q42699 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Catharanthus roseus OX=4058 GN=METE PE=2 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 1.4e-198
Identity = 343/401 (85.54%), Postives = 378/401 (94.26%), Query Frame = 0

Query: 66  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 125
           MASHIVGYPR+GPKRELKFALESFWD KSSA+DLQKVAADLRSSIWKQMA+AGIK+IPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDKKSSAEDLQKVAADLRSSIWKQMADAGIKYIPSN 60

Query: 126 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 185
           TFSYYDQVLDTATMLGAVP RY + GGEIGFD YFSMARGNASVPAMEMTKWFDTNYHYI
Sbjct: 61  TFSYYDQVLDTATMLGAVPPRYNFAGGEIGFDTYFSMARGNASVPAMEMTKWFDTNYHYI 120

Query: 186 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 245
           VPEL PE  F+YASHKAVNE+KEAK LGV+TVPVLVGPV+FLLLSKPAKGVEK+F LLSL
Sbjct: 121 VPELGPEVNFSYASHKAVNEYKEAKELGVDTVPVLVGPVTFLLLSKPAKGVEKTFPLLSL 180

Query: 246 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLNVLI 305
           +DKILPVYKEV+ ELKAAGA+WIQFDEPTLV DL++H L+AF +AYSELESTLSGLNV++
Sbjct: 181 LDKILPVYKEVIGELKAAGASWIQFDEPTLVLDLESHQLEAFTKAYSELESTLSGLNVIV 240

Query: 306 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDGRNIWS 365
           ETYFAD+PAE YK LT+LKGV+G+GFDLVRG +TLDLIKGGFPSGKYLFAGVVDGRNIW+
Sbjct: 241 ETYFADIPAETYKILTALKGVTGFGFDLVRGAKTLDLIKGGFPSGKYLFAGVVDGRNIWA 300

Query: 366 NDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVVE 425
           NDL AS++TL++LE +VGK+K+VVSTSCSLLHTAVDLVNE KLD EIKSWLAFAAQKVVE
Sbjct: 301 NDLAASLSTLQSLEGIVGKDKLVVSTSCSLLHTAVDLVNEPKLDKEIKSWLAFAAQKVVE 360

Query: 426 VNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA 467
           VNALAKAL+G KDEA+FS NAAAQASRKSSPRVTN+AVQKA
Sbjct: 361 VNALAKALAGEKDEAFFSENAAAQASRKSSPRVTNQAVQKA 401

BLAST of Cucsat.G7170.T25 vs. ExPASy Swiss-Prot
Match: Q2QLY5 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0623900 PE=2 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 3.2e-195
Identity = 337/402 (83.83%), Postives = 374/402 (93.03%), Query Frame = 0

Query: 66  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 125
           MASHIVGYPR+GPKRELKFALESFWDGKSSA+DL+KVA DLR+SIWKQMA+AGIK+IPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDGKSSAEDLEKVATDLRASIWKQMADAGIKYIPSN 60

Query: 126 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 185
           TFSYYDQVLDTA MLGAVP RY W GGEIGF  YFSMARGNA+VPAMEMTKWFDTNYH+I
Sbjct: 61  TFSYYDQVLDTAAMLGAVPERYSWTGGEIGFSTYFSMARGNATVPAMEMTKWFDTNYHFI 120

Query: 186 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 245
           VPEL P  KF+Y+SHKAVNE+KEAKALGV+TVPVLVGPVS+LLLSKPAKGVEKSF+LLSL
Sbjct: 121 VPELGPNTKFSYSSHKAVNEYKEAKALGVDTVPVLVGPVSYLLLSKPAKGVEKSFALLSL 180

Query: 246 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLNVLI 305
           +  ILPVYKEV++ELKAAGATWIQFDEPTLV DLD+H L AF+ AY+ELES LSGLNVLI
Sbjct: 181 LSSILPVYKEVIAELKAAGATWIQFDEPTLVLDLDSHQLAAFSAAYTELESALSGLNVLI 240

Query: 306 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIK-GGFPSGKYLFAGVVDGRNIW 365
           ETYFAD+PAE+YKTLTSL  V+ YGFDL+RG +TLDLIK  GFPSGKYLFAGVVDGRNIW
Sbjct: 241 ETYFADIPAESYKTLTSLNSVTAYGFDLIRGAKTLDLIKSAGFPSGKYLFAGVVDGRNIW 300

Query: 366 SNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVV 425
           ++DL AS+TTLE+LE +VGK+K+VVSTSCSL+HTAVDLVNETKLD EIKSWLAFAAQKVV
Sbjct: 301 ADDLAASLTTLESLEAIVGKDKLVVSTSCSLMHTAVDLVNETKLDSEIKSWLAFAAQKVV 360

Query: 426 EVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA 467
           EVNALAKAL+G KDEAYF++NAAAQASR+SSPRVTNE VQKA
Sbjct: 361 EVNALAKALAGQKDEAYFAANAAAQASRRSSPRVTNEEVQKA 402

BLAST of Cucsat.G7170.T25 vs. ExPASy Swiss-Prot
Match: P93263 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Mesembryanthemum crystallinum OX=3544 GN=METE PE=2 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 3.2e-195
Identity = 338/401 (84.29%), Postives = 374/401 (93.27%), Query Frame = 0

Query: 66  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 125
           MASHIVGYPR+GPKRELKFALESFWDGKS+A+DL+KV+ADLRSSIWKQMA+AGIK+IPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDGKSTAEDLKKVSADLRSSIWKQMADAGIKYIPSN 60

Query: 126 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 185
           TFSYYDQVLDT  MLGAVP RYGW GGEI FD+YFSMARGNASVPAMEMTKWFDTNYH+I
Sbjct: 61  TFSYYDQVLDTTAMLGAVPPRYGWTGGEIEFDVYFSMARGNASVPAMEMTKWFDTNYHFI 120

Query: 186 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 245
           VPEL PE  F+YASHKAV E+KEAKALGV+TVPVLVGPVS+LLLSK AKGV+KSF LLSL
Sbjct: 121 VPELGPEVNFSYASHKAVLEYKEAKALGVDTVPVLVGPVSYLLLSKQAKGVDKSFDLLSL 180

Query: 246 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLNVLI 305
           + KILP+YKEVV+ELK AGA+WIQFDEP LV DL++H LQAF+ AY++LESTLSGLNV++
Sbjct: 181 LPKILPIYKEVVAELKEAGASWIQFDEPLLVMDLESHKLQAFSAAYADLESTLSGLNVVV 240

Query: 306 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDGRNIWS 365
           ETYFADV AEAYKTL SLKGV+GYGFDLVRGT+TLDL+K  FPSGKYLFAGVVDGRNIW+
Sbjct: 241 ETYFADVTAEAYKTLISLKGVTGYGFDLVRGTKTLDLVKAEFPSGKYLFAGVVDGRNIWA 300

Query: 366 NDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVVE 425
           NDL AS+ TLEALE VVGK+K+VVSTSCS LHTAVDL+NETKLDDEIKSWLAFAAQKV+E
Sbjct: 301 NDLAASLATLEALEGVVGKDKLVVSTSCSFLHTAVDLINETKLDDEIKSWLAFAAQKVLE 360

Query: 426 VNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA 467
           VNALAKALSG KDEA+FS+NAAA ASRKSSPRVTNEAVQKA
Sbjct: 361 VNALAKALSGQKDEAFFSANAAALASRKSSPRVTNEAVQKA 401

BLAST of Cucsat.G7170.T25 vs. ExPASy Swiss-Prot
Match: Q2QLY4 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0624000 PE=2 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 2.7e-194
Identity = 334/402 (83.08%), Postives = 373/402 (92.79%), Query Frame = 0

Query: 66  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 125
           MASHIVGYPR+GPKRELKFALESFWDGKSSA+DL+KVA DLR+SIWKQMA+AGIK+IPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDGKSSAEDLEKVATDLRASIWKQMADAGIKYIPSN 60

Query: 126 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 185
           TFSYYDQVLDT  MLGAVP RY W GGEIGF  YFSMARGNA+VPAMEMTKWFDTNYH+I
Sbjct: 61  TFSYYDQVLDTTAMLGAVPERYSWTGGEIGFSTYFSMARGNATVPAMEMTKWFDTNYHFI 120

Query: 186 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 245
           VPEL P  KF+Y+SHKAVNE+KEAKALGV+TVPVLVGPVS+LLLSKPAKGVEKSF+LLSL
Sbjct: 121 VPELGPNTKFSYSSHKAVNEYKEAKALGVDTVPVLVGPVSYLLLSKPAKGVEKSFALLSL 180

Query: 246 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLNVLI 305
           +  ILPVYKEV++ELKAAGATWIQFDEPTLV DLD+H L AF+ AY+ELES LSGLNVLI
Sbjct: 181 LSSILPVYKEVIAELKAAGATWIQFDEPTLVLDLDSHQLAAFSAAYTELESALSGLNVLI 240

Query: 306 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIK-GGFPSGKYLFAGVVDGRNIW 365
           ETYFAD+PAE+YKTLTSL  V+ YGFDL+RG++TLDL+K  GFPSGKYLFAGVVDGRNIW
Sbjct: 241 ETYFADIPAESYKTLTSLNSVTAYGFDLIRGSKTLDLVKSAGFPSGKYLFAGVVDGRNIW 300

Query: 366 SNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVV 425
           ++DL AS+TTLE+LE +VGK+K+VVSTSCSL+HTAVDLVNETKLD EIKSWLAFAAQKVV
Sbjct: 301 ADDLAASLTTLESLEAIVGKDKLVVSTSCSLMHTAVDLVNETKLDSEIKSWLAFAAQKVV 360

Query: 426 EVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA 467
           EVNALAKAL+G KDEAYF++N AAQASR+SSPRVTNE VQKA
Sbjct: 361 EVNALAKALAGQKDEAYFAANTAAQASRRSSPRVTNEEVQKA 402

BLAST of Cucsat.G7170.T25 vs. ExPASy Swiss-Prot
Match: O50008 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=MS1 PE=1 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 1.8e-193
Identity = 333/401 (83.04%), Postives = 370/401 (92.27%), Query Frame = 0

Query: 66  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 125
           MASHIVGYPR+GPKRELKFALESFWDGKS+A+DLQKV+ADLRSSIWKQM+ AG KFIPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDGKSTAEDLQKVSADLRSSIWKQMSAAGTKFIPSN 60

Query: 126 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 185
           TF++YDQVLDT  MLGAVP RYG+ GGEIG D+YFSMARGNASVPAMEMTKWFDTNYHYI
Sbjct: 61  TFAHYDQVLDTTAMLGAVPPRYGYTGGEIGLDVYFSMARGNASVPAMEMTKWFDTNYHYI 120

Query: 186 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 245
           VPEL PE  F+YASHKAVNE+KEAKALGV+TVPVLVGPVS+LLLSK AKGV+KSF LLSL
Sbjct: 121 VPELGPEVNFSYASHKAVNEYKEAKALGVDTVPVLVGPVSYLLLSKAAKGVDKSFELLSL 180

Query: 246 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLNVLI 305
           + KILP+YKEV++ELKAAGATWIQ DEP LV DL+   LQAF  AY+ELESTLSGLNVL+
Sbjct: 181 LPKILPIYKEVITELKAAGATWIQLDEPVLVMDLEGQKLQAFTGAYAELESTLSGLNVLV 240

Query: 306 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDGRNIWS 365
           ETYFAD+PAEAYKTLTSLKGV+ +GFDLVRGT+TLDL+K GFP GKYLFAGVVDGRNIW+
Sbjct: 241 ETYFADIPAEAYKTLTSLKGVTAFGFDLVRGTKTLDLVKAGFPEGKYLFAGVVDGRNIWA 300

Query: 366 NDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVVE 425
           ND  AS++TL+ALE +VGK+K+VVSTSCSLLHTAVDL+NETKLDDEIKSWLAFAAQKVVE
Sbjct: 301 NDFAASLSTLQALEGIVGKDKLVVSTSCSLLHTAVDLINETKLDDEIKSWLAFAAQKVVE 360

Query: 426 VNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA 467
           VNALAKAL+G KDEA FS+NAAA ASR+SSPRVTNE VQKA
Sbjct: 361 VNALAKALAGQKDEALFSANAAALASRRSSPRVTNEGVQKA 401

BLAST of Cucsat.G7170.T25 vs. NCBI nr
Match: XP_004144074.1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [Cucumis sativus] >KGN66367.1 hypothetical protein Csa_007688 [Cucumis sativus])

HSP 1 Score: 880 bits (2273), Expect = 9.29e-316
Identity = 460/466 (98.71%), Postives = 462/466 (99.14%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MTQLPLKSLEPFAISSSSSSS   FSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIR+TS
Sbjct: 1   MTQLPLKSLEPFAISSSSSSS---FSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRSTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK
Sbjct: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAF QAYSELESTLSG
Sbjct: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQT+DLIKGGFPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA 466
           QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA 463

BLAST of Cucsat.G7170.T25 vs. NCBI nr
Match: XP_008451024.1 (PREDICTED: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [Cucumis melo])

HSP 1 Score: 875 bits (2262), Expect = 5.93e-314
Identity = 460/466 (98.71%), Postives = 462/466 (99.14%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MTQLPLKSLEPFAISSSSSSSSSS SIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS
Sbjct: 1   MTQLPLKSLEPFAISSSSSSSSSS-SIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMA+AGIK
Sbjct: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMADAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELESTLSG
Sbjct: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESTLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSN+LQASITTLEAL NVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNNLQASITTLEALGNVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA 466
           QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA 465

BLAST of Cucsat.G7170.T25 vs. NCBI nr
Match: XP_038879499.1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 869 bits (2245), Expect = 2.12e-311
Identity = 454/466 (97.42%), Postives = 459/466 (98.50%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MTQLPLKSLEPFAISSSSSSSSS   IFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS
Sbjct: 1   MTQLPLKSLEPFAISSSSSSSSS---IFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMA+AGIK
Sbjct: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMADAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNE+KEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEYKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSL+DKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELES LSG
Sbjct: 241 SLLSLVDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESVLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSNDLQASITTLEALE VVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNDLQASITTLEALEKVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA 466
           QKVVEVNALAKAL+GHKDEAYFS+NAAAQASRKSSPRVTNEAVQKA
Sbjct: 421 QKVVEVNALAKALAGHKDEAYFSANAAAQASRKSSPRVTNEAVQKA 463

BLAST of Cucsat.G7170.T25 vs. NCBI nr
Match: XP_023533330.1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 833 bits (2153), Expect = 1.93e-297
Identity = 433/466 (92.92%), Postives = 446/466 (95.71%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MT LPLKSL+ F ISS SSSS+S     ALRRG IAPFSYLL SSIH LPSLRLSIRTTS
Sbjct: 1   MTLLPLKSLDSFGISSCSSSSTS----IALRRGTIAPFSYLLVSSIHRLPSLRLSIRTTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASH+VGYPRIGPKRELKFALESFWDGKS+ADDLQKVA DLR+SIWKQMA+AGIK
Sbjct: 61  TVVRAMASHVVGYPRIGPKRELKFALESFWDGKSTADDLQKVATDLRASIWKQMADAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFD YFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDTYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNE+KEAKALGVET+PVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEYKEAKALGVETIPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSL+DKILP+YKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELES LSG
Sbjct: 241 SLLSLVDKILPIYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESVLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKG FPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGEFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSNDLQAS+ TLEAL+NVVGKE IVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNDLQASLNTLEALQNVVGKENIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA 466
           QKVVEVNALAKALSGHKDEAYFS+NAAAQASRKSSPRVTNEAVQKA
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSANAAAQASRKSSPRVTNEAVQKA 462

BLAST of Cucsat.G7170.T25 vs. NCBI nr
Match: KAG6590268.1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 831 bits (2147), Expect = 1.57e-296
Identity = 431/466 (92.49%), Postives = 446/466 (95.71%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MT LPLKSL+ F ISS SSSS+S     ALRRG IAPFSYLL SSIH LPSLRLSIRTTS
Sbjct: 1   MTLLPLKSLDSFGISSCSSSSTS----IALRRGTIAPFSYLLVSSIHRLPSLRLSIRTTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASH+VGYPRIGPKRELKFALESFWDGKS+ADDLQKVA DLR+SIWKQMA+AGIK
Sbjct: 61  TVVRAMASHVVGYPRIGPKRELKFALESFWDGKSTADDLQKVATDLRASIWKQMADAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFD+YFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDMYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNE+KEAKALGVET+PVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEYKEAKALGVETIPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSL+DKILP+YKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELES LSG
Sbjct: 241 SLLSLVDKILPIYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESVLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKG FPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGEFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSNDLQAS+ TLEAL+NVVGKE IVVSTSCSLLHTAVDL NETKL+DEIKSWLAFAA
Sbjct: 361 RNIWSNDLQASLNTLEALQNVVGKENIVVSTSCSLLHTAVDLANETKLEDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA 466
           QKVVEVNALAKALSGHKDEAYFS+NAAAQASRKSSPRVTNEAVQKA
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSANAAAQASRKSSPRVTNEAVQKA 462

BLAST of Cucsat.G7170.T25 vs. ExPASy TrEMBL
Match: A0A0A0LZR2 (5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_1G599580 PE=3 SV=1)

HSP 1 Score: 880 bits (2273), Expect = 4.50e-316
Identity = 460/466 (98.71%), Postives = 462/466 (99.14%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MTQLPLKSLEPFAISSSSSSS   FSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIR+TS
Sbjct: 1   MTQLPLKSLEPFAISSSSSSS---FSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRSTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK
Sbjct: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAF QAYSELESTLSG
Sbjct: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQT+DLIKGGFPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA 466
           QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA 463

BLAST of Cucsat.G7170.T25 vs. ExPASy TrEMBL
Match: A0A1S3BQJ8 (5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103492431 PE=3 SV=1)

HSP 1 Score: 875 bits (2262), Expect = 2.87e-314
Identity = 460/466 (98.71%), Postives = 462/466 (99.14%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MTQLPLKSLEPFAISSSSSSSSSS SIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS
Sbjct: 1   MTQLPLKSLEPFAISSSSSSSSSS-SIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMA+AGIK
Sbjct: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMADAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELESTLSG
Sbjct: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESTLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSN+LQASITTLEAL NVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNNLQASITTLEALGNVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA 466
           QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA 465

BLAST of Cucsat.G7170.T25 vs. ExPASy TrEMBL
Match: A0A6J1HAA9 (5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111461556 PE=3 SV=1)

HSP 1 Score: 828 bits (2139), Expect = 1.25e-295
Identity = 430/466 (92.27%), Postives = 443/466 (95.06%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MT LPLKSL+ F ISS SSSS+S     ALRRG I PFSYLL SSIH  PSLRLSIRTTS
Sbjct: 1   MTLLPLKSLDSFGISSCSSSSTS----IALRRGTITPFSYLLVSSIHRRPSLRLSIRTTS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASH+VGYPRIGPKRELKFALESFWDGKS+ADDLQKVA DLR+SIWKQMA+AGIK
Sbjct: 61  TVVRAMASHVVGYPRIGPKRELKFALESFWDGKSTADDLQKVATDLRASIWKQMADAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFD YFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDTYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNE+KEAKALGVET+PVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEYKEAKALGVETIPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSL+DKILP+YKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELES LSG
Sbjct: 241 SLLSLVDKILPIYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESVLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKG FPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGEFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSNDLQAS+ TLEAL+NVVGKE IVVSTSCSLLHTAVDL NETKLDDEIKSWLAFAA
Sbjct: 361 RNIWSNDLQASLNTLEALQNVVGKENIVVSTSCSLLHTAVDLANETKLDDEIKSWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA 466
           QKVVEVNALAKALSGHKDEAYFS+NAAAQASRKSSPRVTNEAVQKA
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSANAAAQASRKSSPRVTNEAVQKA 462

BLAST of Cucsat.G7170.T25 vs. ExPASy TrEMBL
Match: A0A6J1D1J0 (5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111016496 PE=3 SV=1)

HSP 1 Score: 822 bits (2123), Expect = 3.24e-293
Identity = 427/464 (92.03%), Postives = 440/464 (94.83%), Query Frame = 0

Query: 3   QLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTSTV 62
           QL LKS EPFA SS+SS       I ALRRGAI+PF YLLFSSIHHLPS RLSIRTTSTV
Sbjct: 5   QLSLKSHEPFAKSSASS-------IIALRRGAISPFPYLLFSSIHHLPSRRLSIRTTSTV 64

Query: 63  VRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFI 122
           +RAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMA+AGIKFI
Sbjct: 65  IRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMADAGIKFI 124

Query: 123 PSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNY 182
           PSNTFSYYDQVLDTATMLGAVP RYGWNGGEIGFD YFSMARGNASVPAMEMTKWFDTNY
Sbjct: 125 PSNTFSYYDQVLDTATMLGAVPPRYGWNGGEIGFDTYFSMARGNASVPAMEMTKWFDTNY 184

Query: 183 HYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSL 242
           HYIVPELSP+DKF YASHKAVNE+KEAKALGV+T+PVLVGPVSFL+LSKPAKGVEKSFSL
Sbjct: 185 HYIVPELSPDDKFIYASHKAVNEYKEAKALGVDTIPVLVGPVSFLVLSKPAKGVEKSFSL 244

Query: 243 LSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSGLN 302
           LSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAFA AYSELES LSGLN
Sbjct: 245 LSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESVLSGLN 304

Query: 303 VLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDGRN 362
           VLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQT DLIK GFPSGKYLFAGVVDGRN
Sbjct: 305 VLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTFDLIKSGFPSGKYLFAGVVDGRN 364

Query: 363 IWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQK 422
           IWSNDLQAS+ TL+ALEN VGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQK
Sbjct: 365 IWSNDLQASLNTLQALENAVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQK 424

Query: 423 VVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA 466
           V+EVNALAKALSG KDEAYFS+NAAAQASRKSSPRVTNEAVQKA
Sbjct: 425 VIEVNALAKALSGKKDEAYFSTNAAAQASRKSSPRVTNEAVQKA 461

BLAST of Cucsat.G7170.T25 vs. ExPASy TrEMBL
Match: A0A6J1JBY9 (5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111485391 PE=3 SV=1)

HSP 1 Score: 822 bits (2123), Expect = 3.35e-293
Identity = 427/466 (91.63%), Postives = 441/466 (94.64%), Query Frame = 0

Query: 1   MTQLPLKSLEPFAISSSSSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTS 60
           MT LP+KSL+ F IS  SSSS+S     ALRRG IAPFSYLL SSIH LPSLRLSIRT S
Sbjct: 1   MTLLPVKSLDSFGISPCSSSSTS----IALRRGTIAPFSYLLVSSIHRLPSLRLSIRTAS 60

Query: 61  TVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIK 120
           TVVRAMASH+VGYPRIGPKRELKFALESFWDGKS+ADDLQKVA DLR+SIWKQMA+AGIK
Sbjct: 61  TVVRAMASHVVGYPRIGPKRELKFALESFWDGKSTADDLQKVATDLRASIWKQMADAGIK 120

Query: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDT 180
           FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFD YFSMARGNASVPAMEMTKWFDT
Sbjct: 121 FIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDTYFSMARGNASVPAMEMTKWFDT 180

Query: 181 NYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSF 240
           NYHYIVPELSPEDKFTYASHKAVNE+KEAKALGVET+PVLVGPVSFLLLSKPAKGVEKSF
Sbjct: 181 NYHYIVPELSPEDKFTYASHKAVNEYKEAKALGVETIPVLVGPVSFLLLSKPAKGVEKSF 240

Query: 241 SLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFAQAYSELESTLSG 300
           SLLSL+ KILP+YKEVVSELKAAGATWIQFDEPTLVKDLD H LQAFA AYSELES LSG
Sbjct: 241 SLLSLVGKILPIYKEVVSELKAAGATWIQFDEPTLVKDLDTHQLQAFAHAYSELESVLSG 300

Query: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDG 360
           LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKG FPSGKYLFAGVVDG
Sbjct: 301 LNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGKFPSGKYLFAGVVDG 360

Query: 361 RNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAA 420
           RNIWSNDLQAS+ TLEAL+NVVGKE IVVSTSCSLLHTAVDLVNETKLDDEIK WLAFAA
Sbjct: 361 RNIWSNDLQASLNTLEALQNVVGKENIVVSTSCSLLHTAVDLVNETKLDDEIKLWLAFAA 420

Query: 421 QKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKA 466
           QKVVEVNALAKALSGHKDEAYFS+NAAAQASRKSSPRVTNEAVQKA
Sbjct: 421 QKVVEVNALAKALSGHKDEAYFSANAAAQASRKSSPRVTNEAVQKA 462

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q426991.4e-19885.545-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Cathara... [more]
Q2QLY53.2e-19583.835-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 OS=Oryza... [more]
P932633.2e-19584.295-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Mesembr... [more]
Q2QLY42.7e-19483.085-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2 OS=Oryza... [more]
O500081.8e-19383.045-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 OS=Arabi... [more]
Match NameE-valueIdentityDescription
XP_004144074.19.29e-31698.715-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [Cucumis s... [more]
XP_008451024.15.93e-31498.71PREDICTED: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase... [more]
XP_038879499.12.12e-31197.425-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1-like [Be... [more]
XP_023533330.11.93e-29792.925-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1-like [Cu... [more]
KAG6590268.11.57e-29692.495-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase, partial [... [more]
Match NameE-valueIdentityDescription
A0A0A0LZR24.50e-31698.715-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucum... [more]
A0A1S3BQJ82.87e-31498.715-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucum... [more]
A0A6J1HAA91.25e-29592.275-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucur... [more]
A0A6J1D1J03.24e-29392.035-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Momor... [more]
A0A6J1JBY93.35e-29391.635-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase OS=Cucur... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038071UROD/MetE-like superfamilyGENE3D3.20.20.210coord: 66..467
e-value: 3.1E-162
score: 542.2
IPR038071UROD/MetE-like superfamilySUPERFAMILY51726UROD/MetE-likecoord: 68..460
IPR013215Cobalamin-independent methionine synthase MetE, N-terminalPFAMPF08267Meth_synt_1coord: 68..381
e-value: 5.7E-121
score: 403.8
NoneNo IPR availablePANTHERPTHR305195-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE METHYLTRANSFERASEcoord: 38..467
NoneNo IPR availablePANTHERPTHR30519:SF175-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE METHYLTRANSFERASE 3, CHLOROPLASTICcoord: 38..467
NoneNo IPR availableCDDcd03312CIMS_N_terminal_likecoord: 68..432
e-value: 0.0
score: 588.733

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G7170Cucsat.G7170gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G7170.T25.E1Cucsat.G7170.T25.E1exon
Cucsat.G7170.T25.E2Cucsat.G7170.T25.E2exon
Cucsat.G7170.T25.E3Cucsat.G7170.T25.E3exon
Cucsat.G7170.T25.E4Cucsat.G7170.T25.E4exon
Cucsat.G7170.T25.E5Cucsat.G7170.T25.E5exon
Cucsat.G7170.T25.E6Cucsat.G7170.T25.E6exon
Cucsat.G7170.T25.E7Cucsat.G7170.T25.E7exon
Cucsat.G7170.T25.E8Cucsat.G7170.T25.E8exon
Cucsat.G7170.T25.E9Cucsat.G7170.T25.E9exon
Cucsat.G7170.T25.E10Cucsat.G7170.T25.E10exon
Cucsat.G7170.T25.E11Cucsat.G7170.T25.E11exon
Cucsat.G7170.T25.E12Cucsat.G7170.T25.E12exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G7170.T25.C7Cucsat.G7170.T25.C7CDS
Cucsat.G7170.T25.C6Cucsat.G7170.T25.C6CDS
Cucsat.G7170.T25.C5Cucsat.G7170.T25.C5CDS
Cucsat.G7170.T25.C4Cucsat.G7170.T25.C4CDS
Cucsat.G7170.T25.C3Cucsat.G7170.T25.C3CDS
Cucsat.G7170.T25.C2Cucsat.G7170.T25.C2CDS
Cucsat.G7170.T25.C1Cucsat.G7170.T25.C1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G7170.T25Cucsat.G7170.T25-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008652 cellular amino acid biosynthetic process
biological_process GO:0009086 methionine biosynthetic process
biological_process GO:0032259 methylation
cellular_component GO:0009507 chloroplast
cellular_component GO:0005829 cytosol
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003871 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0004045 aminoacyl-tRNA hydrolase activity
molecular_function GO:0008705 methionine synthase activity