Cucsat.G12064 (gene) Cucumber (B10) v3

Overview
NameCucsat.G12064
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionDNA-directed RNA polymerases II and V subunit 8A-like
Locationctg1821: 96111 .. 99968 (+)
RNA-Seq ExpressionCucsat.G12064
SyntenyCucsat.G12064
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGGATTCTCTTAAAAAACTGGTGGCATCTTGTTCATGTGGGTTGAAAATAAGGTGTCAGTTAAAGAAACACTCAAATGGGTTTTTCTTCAAAGCAACATCATAGAGTGTTCTTGATGGATATCTAATACAATCCTTCTCATTATATAAAAGAAGATTCTATGGCCGGATCCTATGTCAATTCATAGTTATTGTGAAGGCCTTTGATGCATTTGTGGAGTCTTCAATATTTCAGTATTTCAAGATGGCCTAATCATCATTTTCCTACCGGTTGAACCACAAAAGGTTTGCTTCTTTACCAAATGGGTCAGTTTTTTTCATTGGGATTGTGTGATTTTGTCATGGGAGTTTCATTCATTAGCCTTTTGGAAAGACGAAAGAGGTTGAAATTCTGAATTTGAGCAGCTTCTTCATGCTTTATTGATGAAAAAAGTGGAGCATAGTGTTGATTCTCACAGCTGAAAGTTAGATGCCTTTAGACGTTATTTAGTTAGAGATGTGATGTGACAACTAGTTTGTTATTGTATTATCATACACTAACATTCAGTTACAACTAAACTAGAACACAAGGAAGCATAATCGTTCAGTACCTTAAGACAATGCCTCCAAACCCACTGTTAACACGCAAATCCTGCATGAACTCAAGAGAAGTTAGATTCATTCTTTATTCAATGTAGAAAGAAATCCGTTAATAGTATTTTATACACAATGCCATCTAACTAAAACCTTAAACAAGCATGAATCCGATTAATCATACAAATACGAAAGCATCTTGCCTCATTTAATGAAGCAACTTAAAAATAATTAAACAAAACTGTACTTTTTGTTCCTTAGTTTTTACTATTTCTATTTGGTCCCTGTTTTTTCAACTTCTACAATTTAAACCCAATTTGTTGGTTTACAATTGAAAGGCATGAATGTCAAGAGTTTCAAGGATTCTGTCATCTAAGTTGTACTTTTTATAAAGCATTGTATGACCAAACTAAGAAATTAGGATTACACATTTGGAAAGTTCCTCGTTTTGGACATTCTCCACTTGGGAAGCAAAACCATCCATTAGATTTTCTCCAGCATCTAAACACAATATAACCTATTTTTCTATAGACTTCCATTCCTGTTCCCTAAGCATCGAATTCATATTGATACCAATCATCATATGTGAATGCATCTTCAAAATTCCCAGCAAGATCGTATAGAACTAAGTCAATACATCCAACAATACATTTCGTACAATAACTAACGAAGTGGAGAGGGAATGCAAACATACAAACTAATTAACCCTGTTAGAGAACTAAAATGGTTTCTTTATATGCCTACACGAACCAATCACTGCGAAAATAAGCAACATAGTAAAAAATATGAAAACAAAAGAAAAGGAGAGAATCAGGTACGTTCTTTTAACAAGCCAAATCGTGCAAGCTTGCGTCCTGTACACCTTTTTGCATCACACTGCCCAAAATCCTGAATCAACCACAAAACACCATCAATTTCAATTTACTTACAACTTTGTAAGCTCAAGCTTTCATTCCCATTTGTAACAACAGCACAACAGTAACAAGTTTAAGGATGACACAATTCAGAATTCAACAACAAAAACGCGAAGGAAAAAAAAAATGTTAAGCACACACCCACATGGCAAGCTGAATATTTGGACGCGTTGGCTCCTCTTCATTGGCTGCAACAAAAAGGAACTCGTCAAGAACCTAAATCCAAAGTCTCTCCACTATAAAAGGGCTTCTTTAAACAGAACAAAATCACCCATGTAGAAGAAAACTTCAGTGAGACAAAGTAGGCACCTGGGTCGACCGGCAAACACTCATCCTCTCTGAAAAAAAAGAAATAAACAAAACAAATGATCGATATAATGAAAAGACGAATCAGAGGAAAAGGGTAAGAGTATATGTAAATACCCAATAAATTTATGGCCCCGAGTAGATTGTCCGCGACGAGGATGATGATTTTTGAAGCGTCTAGTCTTGTTATGGCTCATTCTGAGGCAGTTTAGTGAAGTGGGATTCGGTGATGTTGGGTTCTCCTTTACTTCTAGACCTGTTCAGAAAAATCATTGGAATAATAATAACATGACCTGGGTCGGTTAAATAAGTGTAGGGTTGGCCCAAGCCAATATAAACCTAAACCCTGAACCTTTTTACCGAAATTCCTTCAATCACCCAGTCGCCCACACGCTCCGTCGCCGCGCCCCTTTCCGAAATTCCTTCAATCTTCTCAAGGGTAGTGTTAAGAGGAAGAGTCCTAAGAAGCTACTAAAGGAAGAAGAGCTCTCAGAAAGTAGGAAAGTTTCCCTGACTATTTTCTTCCCCTTTACTTGATATTATTGTTTGTCCTTATTTTTCTGCATTATCTCTTCTGGTTTTGGTCGTCAAATTCATTTGCATAATTGTAATTATCCATAGTTTATGATTAGTTTTTACAATTCGAAAGGTTAAAATTTTGGAGTTAAGATAGAATGACCAATTTCTAAAAACCACAAAACATGCTGTGGGTGTTGTAATTATACTAACAAAGACTTTCTTTGTTCAAGCTTCAATTCTTCCTACCTTGTAAATATAAACTATGAACTCCCAACGTACTTTTTCTTAAAAAAATATTAATAGTAAACAATTTTACTTTTAATACATATGTATAAGTATATAAACGGCGATGGAGTAAGGTTTGTCTTTGTCATGCCATTGAATTTTAAGCTTCGAATATGTTAAATTCATATTTGTTAATAGAAAAATAAGGAAAAAGTGTACCCCTAAAATAAAACCCTAAACCCTTTCATATTCCTCTCACTCTTGTTCCACCTCCGTCTTTGCGCCTTTGCGCCAAATCTCTATCTATGTATCACTCTCAATTGAGCCTTGATCATCTGTAAATTTTAGTTTTTGCTTTTTTTTTTTCCTGCCTTTCTTCCCATATCTAATATTTTTTTATGTTGTCTACCAACTCAGATTTGCGTTCTGAGAAATAAATTCTCCATAAACATGGCGGGGCTTCTATTTAATGATATTTTCAAGGTCAAGTGTGTCAACCCAGATGGTAAAAAGTATGATAAAGGTACTTCCCTCAAGCTTCTTATTGCTCTTTCATTCTTTTAATTCTTAGTTTCTGGTTTTTGAACTTTTGAGCCTCCGTTGAGATGTTGGGAATCACAAAACTCACTTGTTACAAGACGCCGCTCTCTCAATAGACGAGCTACTCTCATTGCCAATTGGTTTTGAGATGCCCATGCTATAAAATAGCCCCCTTTGTGGACTTGAAACTTCTTCACTGTATTGATTTTAGCGTGAAGGATTTTACAGGACGCACATTGATTGCATAAAATTGTTGTTCTAGTTTCTAGAATTGAAGCACGGAGTGAGAAGCTTTCGATGCATATGCTGCTGGATGTAAATACAGAGATATATCCTATTGATGAAGGTGAGAAGCTTCTGATGGTCTTGTCTCCCACGTTGAACTATGATGGATCTCCTGTTACCAGCTATAAAGATCAGGTAATCCCTCCCAATGTTTTATTTAACTTTGTTGATGGAGGATTCGGGCTGTTCCTTTTCTGTTTGCATTGTTTAAAAGACCAGACAGGTAAAAAAAAAAAAACTGTTGGCATCTGCGTCCATATCAATTTTTCCATGAGTAGAAATGGTAGATATATAATAATATTTTATAGGAATTACTAAAGATATCATTAATAACTCCTCAAGGTATCTCTGCGACTGAACATTTGGAGAAGGATCTTATCAAGGAGTCTAAAAAAAACTAGAAAAAAGGGTCTGCAAATTGCAGGGCAAGTATCCATTGGTTTCGTTCTGTCAAGGCCGTGCTTGTGCTAGCCATGTCTCTTGAAAACAAATGTGAGGAAAGGAATGTGACTTATTTAGGCTCCTTATGACTTGTGTCTAAGGCTTGTGATGCATGACATAGATTTGTTGAGTCTTGAATATGCGCTTGGTGTTCCAACTTCCCTTAAGACACTGAGGAGCATCTTATTAATCTCAATAGAGTCCTTCAAGTTTTTGAATTTCTTGAACCAATATTTCGGAGGAGGCCCTTGTTTTAATCTACCTTGTTGCATCTATGGCTCAAAAGAAATTGGAGAATCTTTAGAGATATAGAAAAGTCATGAGAGGTGGTTTAGAGTGAGTTGCATTTCAATGCCTCTCTGTAGGCGTGCAGAGAGCTTGTTCCACTTCTTTTTGAAAAAAACTGTATTGTTCTTTTAATTTTGTTCTCTTTGCTTCTATGTTATGCTGTACTGTCATTTATTTTTATCTGCTCCTTTTGCTCTTTCATTACATTTATTTCTTATGAAGAAAATATGCACTTGTTAATTTATTTAATTTTATTTTAAAGGTTCGTAACTTTGTATAAATTGCCAACACAAGCATAGCGTAGTTGGCATCTGTATGTTCCCAAGGACAAGATGTTCGTGTTCAAATTCCCCACCCCAAGTTGTACTTAAAAAGACTTTTTTTTAAAGAAACTTCGTATAGACTATTTTTATAATCAAAATTTTGCAGATGTTGGATAGCATTAGAAATGTAGATACACTTTTAACATAACGTTAACAAGAGAAACTTTGACAAACCTGGTTTAATTCAGTTTCCATGATCGTAATTGTAAACATTACACATTTGAAATTTTAGTGGTAATATCCTTCAAATGAACTTAATTTTTGGCAGGAAGGGAAGAAATCTCTAGCTGATAAGTTTGAATACATCATGCATGGTAAAACATACAAACTTTCAGATGAAGGCTCAGGTTCAGACTTGAAAGTGTAAGTGACCTAACATTCTCACGATTTTCTGCAACAGTTCAAATCTGTCATTTTCTTGTCAAATCTTCTATGAATAAAACAAGTACCCTAACCAATTGGAGAGGGCAGTTAACCTCTCCTTCCCTACAATTAGACCGTGTACCATGTCCATGACTCCACTAACCGATTGCTTTGGGGATTATACCCCTTTTTACTCTTTCTAGCATAAGTACTAATAATTGAGTCGTATGAGGCGTTTGCAACTCAATTAATAAATTGTTTCACATGTCTCGTAAATCGTTGGCTCTGATGTTGTTTGGTTGGAATGATTGTATATGCAGGGAGGTTTATGCATCTTTTGGAGGACTCCAAATGCTGTTGAAGGGAGATCCCATCCACTGTGCCAAATTCAGGGTGGATCAGAACATGTTTTTGCTCCTTCGAAAACTCACATAAACCATACAAATCTCTAGTATGGCCTTTTGTTATTACAAAAATTGTGATAACTCTGCATAACTATTGATCATATCTGCAGATAAATTTTGAATTGAATTCTTTTACCAGATCGATCTTCATAGTCGTTGTATGGTTAGTGCTATCTTAAAATTTTCTAGTGGTTTGTTTTTTTCACTCAACATTAGTGAGTGTTGCAACAATTCTCCTTAGATTGTAATTTAAATTAATTGAACTATATTATTGACTAATAATT

Coding sequence (CDS)

ATGGCGGGGCTTCTATTTAATGATATTTTCAAGGTCAAGTGTGTCAACCCAGATGGTAAAAAGTATGATAAAGTTTCTAGAATTGAAGCACGGAGTGAGAAGCTTTCGATGCATATGCTGCTGGATGTAAATACAGAGATATATCCTATTGATGAAGGTGAGAAGCTTCTGATGGTCTTGTCTCCCACGTTGAACTATGATGGATCTCCTGTTACCAGCTATAAAGATCAGGAAGGGAAGAAATCTCTAGCTGATAAGTTTGAATACATCATGCATGGTAAAACATACAAACTTTCAGATGAAGGCTCAGGTTCAGACTTGAAAGTGGAGGTTTATGCATCTTTTGGAGGACTCCAAATGCTGTTGAAGGGAGATCCCATCCACTGTGCCAAATTCAGGGTGGATCAGAACATGTTTTTGCTCCTTCGAAAACTCACATAA

Protein sequence

MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVLSPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQMLLKGDPIHCAKFRVDQNMFLLLRKLT
Homology
[Close]
BLAST of Cucsat.G12064 vs. ExPASy Swiss-Prot
Match: Q9M1A8 (DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=3702 GN=NRPB8B PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 9.3e-18
Identity = 40/71 (56.34%), Postives = 54/71 (76.06%), Query Frame = 0 

Query: 4  LLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVLSPT 63
          ++F DIF V  ++PDGKK+DKV+R+EARS  L M M LDVNTE+YP+  G+K  + ++PT
Sbjct: 6  IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64 LNYDGSPVTSY 75
          LN DG+P T Y
Sbjct: 66 LNLDGTPDTGY 76

[Close]
BLAST of Cucsat.G12064 vs. ExPASy Swiss-Prot
Match: O81097 (DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702 GN=NRPB8A PE=1 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 3.5e-17
Identity = 40/71 (56.34%), Postives = 53/71 (74.65%), Query Frame = 0 

Query: 4  LLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVLSPT 63
          +LF DIF V  ++PDGKK+DKV+R++A S  L M M LDVNTE+YP+  G+K  + L+PT
Sbjct: 6  ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64 LNYDGSPVTSY 75
          LN DG+P T Y
Sbjct: 66 LNLDGTPDTGY 76

[Close]
BLAST of Cucsat.G12064 vs. ExPASy Swiss-Prot
Match: P52434 (DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Homo sapiens OX=9606 GN=POLR2H PE=1 SV=4)

HSP 1 Score: 84.0 bits (206), Expect = 1.5e-15
Identity = 36/69 (52.17%), Postives = 52/69 (75.36%), Query Frame = 0 

Query: 1  MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
          MAG+LF DIF VK ++P+GKK+D+VSR+   SE   M ++LDVN +IYP+D G+K  +V+
Sbjct: 1  MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVI 60

Query: 61 SPTLNYDGS 70
          + TL  DG+
Sbjct: 61 ASTLYEDGT 69

[Close]
BLAST of Cucsat.G12064 vs. ExPASy Swiss-Prot
Match: Q923G2 (DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Mus musculus OX=10090 GN=Polr2h PE=1 SV=3)

HSP 1 Score: 84.0 bits (206), Expect = 1.5e-15
Identity = 36/69 (52.17%), Postives = 52/69 (75.36%), Query Frame = 0 

Query: 1  MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
          MAG+LF DIF VK ++P+GKK+D+VSR+   SE   M ++LDVN +IYP+D G+K  +V+
Sbjct: 1  MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVI 60

Query: 61 SPTLNYDGS 70
          + TL  DG+
Sbjct: 61 ASTLYEDGT 69

[Close]
BLAST of Cucsat.G12064 vs. ExPASy Swiss-Prot
Match: Q19826 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis elegans OX=6239 GN=rpb-8 PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 9.6e-15
Identity = 33/68 (48.53%), Postives = 53/68 (77.94%), Query Frame = 0 

Query: 1  MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
          MAG++F+D+FKVK V+PDGKK+D+VSR    +E   M +++D+N++IYP+ + +K+ +VL
Sbjct: 1  MAGIIFDDMFKVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKVRLVL 60

Query: 61 SPTLNYDG 69
          + TL  DG
Sbjct: 61 ATTLREDG 68

[Close]
BLAST of Cucsat.G12064 vs. NCBI nr
Match: XP_031740213.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like isoform X2 [Cucumis sativus] >XP_031745817.1 DNA-directed RNA polymerases II, IV and V subunit 8B isoform X2 [Cucumis sativus])

HSP 1 Score: 159 bits (403), Expect = 1.21e-47
Identity = 77/77 (100.00%), Postives = 77/77 (100.00%), Query Frame = 0 

Query: 1  MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
          MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL
Sbjct: 1  MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60

Query: 61 SPTLNYDGSPVTSYKDQ 77
          SPTLNYDGSPVTSYKDQ
Sbjct: 61 SPTLNYDGSPVTSYKDQ 77

[Close]
BLAST of Cucsat.G12064 vs. NCBI nr
Match: XP_004149143.1 (DNA-directed RNA polymerases II, IV and V subunit 8B isoform X1 [Cucumis sativus] >XP_011653128.1 DNA-directed RNA polymerases II, IV and V subunit 8B isoform X1 [Cucumis sativus] >XP_011653129.1 DNA-directed RNA polymerases II, IV and V subunit 8B isoform X1 [Cucumis sativus] >XP_031740209.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like isoform X1 [Cucumis sativus] >XP_031740210.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like isoform X1 [Cucumis sativus] >XP_031740211.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like isoform X1 [Cucumis sativus] >XP_031740212.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like isoform X1 [Cucumis sativus] >XP_031745816.1 DNA-directed RNA polymerases II, IV and V subunit 8B isoform X1 [Cucumis sativus] >KAE8637582.1 hypothetical protein CSA_017859 [Cucumis sativus] >KGN53231.1 hypothetical protein Csa_015226 [Cucumis sativus])

HSP 1 Score: 159 bits (403), Expect = 2.73e-47
Identity = 77/77 (100.00%), Postives = 77/77 (100.00%), Query Frame = 0 

Query: 1  MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
          MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL
Sbjct: 1  MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60

Query: 61 SPTLNYDGSPVTSYKDQ 77
          SPTLNYDGSPVTSYKDQ
Sbjct: 61 SPTLNYDGSPVTSYKDQ 77

[Close]
BLAST of Cucsat.G12064 vs. NCBI nr
Match: XP_008453774.1 (PREDICTED: DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo] >KAA0044730.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo var. makuwa] >TYK16730.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo var. makuwa])

HSP 1 Score: 154 bits (390), Expect = 2.58e-45
Identity = 74/77 (96.10%), Postives = 76/77 (98.70%), Query Frame = 0 

Query: 1  MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
          MAGLLFND+FKVKCVNPDGKKYDKVSRI+ARSEKLSM MLLDVNTEIYPIDEGEKLLMVL
Sbjct: 1  MAGLLFNDVFKVKCVNPDGKKYDKVSRIDARSEKLSMSMLLDVNTEIYPIDEGEKLLMVL 60

Query: 61 SPTLNYDGSPVTSYKDQ 77
          SPTLNYDGSPVTSYKDQ
Sbjct: 61 SPTLNYDGSPVTSYKDQ 77

[Close]
BLAST of Cucsat.G12064 vs. NCBI nr
Match: XP_022929200.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >XP_022929202.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >XP_022985172.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_023551974.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita pepo subsp. pepo] >KAG6577274.1 DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015358.1 DNA-directed RNA polymerases II, IV and V subunit 8B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 147 bits (371), Expect = 1.93e-42
Identity = 70/77 (90.91%), Postives = 74/77 (96.10%), Query Frame = 0 

Query: 1  MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
          MA LLFND+FKVKCVNPDGKKYDKVSRIEARSEKLSM+MLLDVNTEIYPIDEGEK+LMVL
Sbjct: 1  MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61 SPTLNYDGSPVTSYKDQ 77
          SPTLNYDG+PVTSY  Q
Sbjct: 61 SPTLNYDGAPVTSYSGQ 77

[Close]
BLAST of Cucsat.G12064 vs. NCBI nr
Match: XP_022942165.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata])

HSP 1 Score: 144 bits (364), Expect = 2.30e-41
Identity = 69/77 (89.61%), Postives = 74/77 (96.10%), Query Frame = 0 

Query: 1  MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
          MA LLF+DIFKVKCVNPDGKKYDKVSRIEARSEKLSM+MLLDVNTEIYPIDEG+K+LMVL
Sbjct: 1  MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61 SPTLNYDGSPVTSYKDQ 77
          SPTLNYDG+PVTSY  Q
Sbjct: 61 SPTLNYDGAPVTSYSGQ 77

[Close]
BLAST of Cucsat.G12064 vs. ExPASy TrEMBL
Match: A0A0A0KZC2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G031020 PE=3 SV=1)

HSP 1 Score: 159 bits (403), Expect = 1.32e-47
Identity = 77/77 (100.00%), Postives = 77/77 (100.00%), Query Frame = 0 

Query: 1  MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
          MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL
Sbjct: 1  MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60

Query: 61 SPTLNYDGSPVTSYKDQ 77
          SPTLNYDGSPVTSYKDQ
Sbjct: 61 SPTLNYDGSPVTSYKDQ 77

[Close]
BLAST of Cucsat.G12064 vs. ExPASy TrEMBL
Match: A0A5A7TNG5 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G005690 PE=3 SV=1)

HSP 1 Score: 154 bits (390), Expect = 1.25e-45
Identity = 74/77 (96.10%), Postives = 76/77 (98.70%), Query Frame = 0 

Query: 1  MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
          MAGLLFND+FKVKCVNPDGKKYDKVSRI+ARSEKLSM MLLDVNTEIYPIDEGEKLLMVL
Sbjct: 1  MAGLLFNDVFKVKCVNPDGKKYDKVSRIDARSEKLSMSMLLDVNTEIYPIDEGEKLLMVL 60

Query: 61 SPTLNYDGSPVTSYKDQ 77
          SPTLNYDGSPVTSYKDQ
Sbjct: 61 SPTLNYDGSPVTSYKDQ 77

[Close]
BLAST of Cucsat.G12064 vs. ExPASy TrEMBL
Match: A0A1S3BY97 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo OX=3656 GN=LOC103494404 PE=3 SV=1)

HSP 1 Score: 154 bits (390), Expect = 1.25e-45
Identity = 74/77 (96.10%), Postives = 76/77 (98.70%), Query Frame = 0 

Query: 1  MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
          MAGLLFND+FKVKCVNPDGKKYDKVSRI+ARSEKLSM MLLDVNTEIYPIDEGEKLLMVL
Sbjct: 1  MAGLLFNDVFKVKCVNPDGKKYDKVSRIDARSEKLSMSMLLDVNTEIYPIDEGEKLLMVL 60

Query: 61 SPTLNYDGSPVTSYKDQ 77
          SPTLNYDGSPVTSYKDQ
Sbjct: 61 SPTLNYDGSPVTSYKDQ 77

[Close]
BLAST of Cucsat.G12064 vs. ExPASy TrEMBL
Match: A0A6J1ETP8 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata OX=3662 GN=LOC111435865 PE=3 SV=1)

HSP 1 Score: 147 bits (371), Expect = 9.34e-43
Identity = 70/77 (90.91%), Postives = 74/77 (96.10%), Query Frame = 0 

Query: 1  MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
          MA LLFND+FKVKCVNPDGKKYDKVSRIEARSEKLSM+MLLDVNTEIYPIDEGEK+LMVL
Sbjct: 1  MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61 SPTLNYDGSPVTSYKDQ 77
          SPTLNYDG+PVTSY  Q
Sbjct: 61 SPTLNYDGAPVTSYSGQ 77

[Close]
BLAST of Cucsat.G12064 vs. ExPASy TrEMBL
Match: A0A6J1J454 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX=3661 GN=LOC111483257 PE=3 SV=1)

HSP 1 Score: 147 bits (371), Expect = 9.34e-43
Identity = 70/77 (90.91%), Postives = 74/77 (96.10%), Query Frame = 0 

Query: 1  MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
          MA LLFND+FKVKCVNPDGKKYDKVSRIEARSEKLSM+MLLDVNTEIYPIDEGEK+LMVL
Sbjct: 1  MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61 SPTLNYDGSPVTSYKDQ 77
          SPTLNYDG+PVTSY  Q
Sbjct: 61 SPTLNYDGAPVTSYSGQ 77

The following BLAST results are available for this feature:
BLAST of Cucsat.G12064 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9M1A89.3e-1856.34DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=... [more]
O810973.5e-1756.34DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702... [more]
P524341.5e-1552.17DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Homo sapiens OX=96... [more]
Q923G21.5e-1552.17DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Mus musculus OX=10... [more]
Q198269.6e-1548.53Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab... [more]
BLAST of Cucsat.G12064 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_031740213.11.21e-47100.00DNA-directed RNA polymerases II, IV and V subunit 8B-like isoform X2 [Cucumis sa... [more]
XP_004149143.12.73e-47100.00DNA-directed RNA polymerases II, IV and V subunit 8B isoform X1 [Cucumis sativus... [more]
XP_008453774.12.58e-4596.10PREDICTED: DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo] ... [more]
XP_022929200.11.93e-4290.91DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >... [more]
XP_022942165.12.30e-4189.61DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata][more]
BLAST of Cucsat.G12064 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KZC21.32e-47100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G031020 PE=3 SV=1[more]
A0A5A7TNG51.25e-4596.10DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo var. makuw... [more]
A0A1S3BY971.25e-4596.10DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo OX=3656 GN... [more]
A0A6J1ETP89.34e-4390.91DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata ... [more]
A0A6J1J4549.34e-4390.91DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005570RNA polymerase, Rpb8SMARTSM00658rpol8neucoord: 2..144
e-value: 5.4E-60
score: 215.3
IPR005570RNA polymerase, Rpb8PFAMPF03870RNA_pol_Rpb8coord: 7..144
e-value: 4.1E-50
score: 169.6
IPR005570RNA polymerase, Rpb8PIRSFPIRSF000779RPB8coord: 1..146
e-value: 6.0E-48
score: 160.0
IPR005570RNA polymerase, Rpb8PANTHERPTHR10917DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3coord: 1..145
NoneNo IPR availableGENE3D2.40.50.140coord: 6..146
e-value: 6.7E-54
score: 183.8
NoneNo IPR availablePANTHERPTHR10917:SF1DNA-DIRECTED RNA POLYMERASE I, IIcoord: 1..145
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 3..144

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G12064.T6Cucsat.G12064.T6mRNA
Cucsat.G12064.T14Cucsat.G12064.T14mRNA
Cucsat.G12064.T7Cucsat.G12064.T7mRNA
Cucsat.G12064.T1Cucsat.G12064.T1mRNA
Cucsat.G12064.T10Cucsat.G12064.T10mRNA
Cucsat.G12064.T3Cucsat.G12064.T3mRNA
Cucsat.G12064.T2Cucsat.G12064.T2mRNA
Cucsat.G12064.T12Cucsat.G12064.T12mRNA
Cucsat.G12064.T8Cucsat.G12064.T8mRNA
Cucsat.G12064.T13Cucsat.G12064.T13mRNA
Cucsat.G12064.T9Cucsat.G12064.T9mRNA
Cucsat.G12064.T5Cucsat.G12064.T5mRNA
Cucsat.G12064.T4Cucsat.G12064.T4mRNA
Cucsat.G12064.T11Cucsat.G12064.T11mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005736 RNA polymerase I complex
cellular_component GO:0005665 RNA polymerase II, core complex
cellular_component GO:0005666 RNA polymerase III complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity