Cucsat.G12064.T5 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G12064.T5
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionDNA-directed RNA polymerases II and V subunit 8A-like
Locationctg1821: 96693 .. 99968 (+)
RNA-Seq ExpressionCucsat.G12064.T5
SyntenyCucsat.G12064.T5
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAAGAGGAAGAGTCCTAAGAAGCTACTAAAGGAAGAAGAGCTCTCAGAAAGTAGGAAAGTTTCCCTGACTATTTTCTTCCCCTTTACTTGATATTATTGTTTGTCCTTATTTTTCTGCATTATCTCTTCTGGTTTTGGTCGTCAAATTCATTTGCATAATTGTAATTATCCATAGTTTATGATTAGTTTTTACAATTCGAAAGGTTAAAATTTTGGAGTTAAGATAGAATGACCAATTTCTAAAAACCACAAAACATGCTGTGGGTGTTGTAATTATACTAACAAAGACTTTCTTTGTTCAAGCTTCAATTCTTCCTACCTTGTAAATATAAACTATGAACTCCCAACGTACTTTTTCTTAAAAAAATATTAATAGTAAACAATTTTACTTTTAATACATATGTATAAGTATATAAACGGCGATGGAGTAAGGTTTGTCTTTGTCATGCCATTGAATTTTAAGCTTCGAATATGTTAAATTCATATTTGTTAATAGAAAAATAAGGAAAAAGTGTACCCCTAAAATAAAACCCTAAACCCTTTCATATTCCTCTCACTCTTGTTCCACCTCCGTCTTTGCGCCTTTGCGCCAAATCTCTATCTATGTATCACTCTCAATTGAGCCTTGATCATCTGTAAATTTTAGTTTTTGCTTTTTTTTTTTCCTGCCTTTCTTCCCATATCTAATATTTTTTTATGTTGTCTACCAACTCAGATTTGCGTTCTGAGAAATAAATTCTCCATAAACATGGCGGGGCTTCTATTTAATGATATTTTCAAGGTCAAGTGTGTCAACCCAGATGGTAAAAAGTATGATAAAGGTACTTCCCTCAAGCTTCTTATTGCTCTTTCATTCTTTTAATTCTTAGTTTCTGGTTTTTGAACTTTTGAGCCTCCGTTGAGATGTTGGGAATCACAAAACTCACTTGTTACAAGACGCCGCTCTCTCAATAGACGAGCTACTCTCATTGCCAATTGGTTTTGAGATGCCCATGCTATAAAATAGCCCCCTTTGTGGACTTGAAACTTCTTCACTGTATTGATTTTAGCGTGAAGGATTTTACAGGACGCACATTGATTGCATAAAATTGTTGTTCTAGTTTCTAGAATTGAAGCACGGAGTGAGAAGCTTTCGATGCATATGCTGCTGGATGTAAATACAGAGATATATCCTATTGATGAAGGTGAGAAGCTTCTGATGGTCTTGTCTCCCACGTTGAACTATGATGGATCTCCTGTTACCAGCTATAAAGATCAGGTAATCCCTCCCAATGTTTTATTTAACTTTGTTGATGGAGGATTCGGGCTGTTCCTTTTCTGTTTGCATTGTTTAAAAGACCAGACAGGTAAAAAAAAAAAAACTGTTGGCATCTGCGTCCATATCAATTTTTCCATGAGTAGAAATGGTAGATATATAATAATATTTTATAGGAATTACTAAAGATATCATTAATAACTCCTCAAGGTATCTCTGCGACTGAACATTTGGAGAAGGATCTTATCAAGGAGTCTAAAAAAAACTAGAAAAAAGGGTCTGCAAATTGCAGGGCAAGTATCCATTGGTTTCGTTCTGTCAAGGCCGTGCTTGTGCTAGCCATGTCTCTTGAAAACAAATGTGAGGAAAGGAATGTGACTTATTTAGGCTCCTTATGACTTGTGTCTAAGGCTTGTGATGCATGACATAGATTTGTTGAGTCTTGAATATGCGCTTGGTGTTCCAACTTCCCTTAAGACACTGAGGAGCATCTTATTAATCTCAATAGAGTCCTTCAAGTTTTTGAATTTCTTGAACCAATATTTCGGAGGAGGCCCTTGTTTTAATCTACCTTGTTGCATCTATGGCTCAAAAGAAATTGGAGAATCTTTAGAGATATAGAAAAGTCATGAGAGGTGGTTTAGAGTGAGTTGCATTTCAATGCCTCTCTGTAGGCGTGCAGAGAGCTTGTTCCACTTCTTTTTGAAAAAAACTGTATTGTTCTTTTAATTTTGTTCTCTTTGCTTCTATGTTATGCTGTACTGTCATTTATTTTTATCTGCTCCTTTTGCTCTTTCATTACATTTATTTCTTATGAAGAAAATATGCACTTGTTAATTTATTTAATTTTATTTTAAAGGTTCGTAACTTTGTATAAATTGCCAACACAAGCATAGCGTAGTTGGCATCTGTATGTTCCCAAGGACAAGATGTTCGTGTTCAAATTCCCCACCCCAAGTTGTACTTAAAAAGACTTTTTTTTAAAGAAACTTCGTATAGACTATTTTTATAATCAAAATTTTGCAGATGTTGGATAGCATTAGAAATGTAGATACACTTTTAACATAACGTTAACAAGAGAAACTTTGACAAACCTGGTTTAATTCAGTTTCCATGATCGTAATTGTAAACATTACACATTTGAAATTTTAGTGGTAATATCCTTCAAATGAACTTAATTTTTGGCAGGAAGGGAAGAAATCTCTAGCTGATAAGTTTGAATACATCATGCATGGTAAAACATACAAACTTTCAGATGAAGGCTCAGGTTCAGACTTGAAAGTGTAAGTGACCTAACATTCTCACGATTTTCTGCAACAGTTCAAATCTGTCATTTTCTTGTCAAATCTTCTATGAATAAAACAAGTACCCTAACCAATTGGAGAGGGCAGTTAACCTCTCCTTCCCTACAATTAGACCGTGTACCATGTCCATGACTCCACTAACCGATTGCTTTGGGGATTATACCCCTTTTTACTCTTTCTAGCATAAGTACTAATAATTGAGTCGTATGAGGCGTTTGCAACTCAATTAATAAATTGTTTCACATGTCTCGTAAATCGTTGGCTCTGATGTTGTTTGGTTGGAATGATTGTATATGCAGGGAGGTTTATGCATCTTTTGGAGGACTCCAAATGCTGTTGAAGGGAGATCCCATCCACTGTGCCAAATTCAGGGTGGATCAGAACATGTTTTTGCTCCTTCGAAAACTCACATAAACCATACAAATCTCTAGTATGGCCTTTTGTTATTACAAAAATTGTGATAACTCTGCATAACTATTGATCATATCTGCAGATAAATTTTGAATTGAATTCTTTTACCAGATCGATCTTCATAGTCGTTGTATGGTTAGTGCTATCTTAAAATTTTCTAGTGGTTTGTTTTTTTCACTCAACATTAGTGAGTGTTGCAACAATTCTCCTTAGATTGTAATTTAAATTAATTGAACTATATTATTGACTAATAATTATAGTTGAAGGAAAAATAGACCTAAAAATGAAAGGATTAGGTAGCAAAG

Coding sequence (CDS)

ATGGCGGGGCTTCTATTTAATGATATTTTCAAGGTCAAGTGTGTCAACCCAGATGGTAAAAAGTATGATAAAGTTTCTAGAATTGAAGCACGGAGTGAGAAGCTTTCGATGCATATGCTGCTGGATGTAAATACAGAGATATATCCTATTGATGAAGGTGAGAAGCTTCTGATGGTCTTGTCTCCCACGTTGAACTATGATGGATCTCCTGTTACCAGCTATAAAGATCAGGAAGGGAAGAAATCTCTAGCTGATAAGTTTGAATACATCATGCATGGTAAAACATACAAACTTTCAGATGAAGGCTCAGGTTCAGACTTGAAAGTGGAGGTTTATGCATCTTTTGGAGGACTCCAAATGCTGTTGAAGGGAGATCCCATCCACTGTGCCAAATTCAGGGTGGATCAGAACATGTTTTTGCTCCTTCGAAAACTCACATAA

Protein sequence

MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVLSPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQMLLKGDPIHCAKFRVDQNMFLLLRKLT
Homology
[Close]
BLAST of Cucsat.G12064.T5 vs. ExPASy Swiss-Prot
Match: Q9M1A8 (DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=3702 GN=NRPB8B PE=1 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 4.4e-42
Identity = 82/142 (57.75%), Postives = 105/142 (73.94%), Query Frame = 0 

Query: 4   LLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVLSPT 63
           ++F DIF V  ++PDGKK+DKV+R+EARS  L M M LDVNTE+YP+  G+K  + ++PT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQMLLK 123
           LN DG+P T Y     KK+LADK+EYIMHGK YK+S E  G   K E+Y SFGGL MLL+
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLQ 125

Query: 124 GDPIHCAKFRVDQNMFLLLRKL 146
           GDP H + F +DQ +FLL+RKL
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]
BLAST of Cucsat.G12064.T5 vs. ExPASy Swiss-Prot
Match: O81097 (DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702 GN=NRPB8A PE=1 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 5.8e-42
Identity = 83/142 (58.45%), Postives = 104/142 (73.24%), Query Frame = 0 

Query: 4   LLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVLSPT 63
           +LF DIF V  ++PDGKK+DKV+R++A S  L M M LDVNTE+YP+  G+K  + L+PT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQMLLK 123
           LN DG+P T Y     KK+LADK+EYIMHGK YK+S E  G   K E+Y SFGGL MLLK
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLK 125

Query: 124 GDPIHCAKFRVDQNMFLLLRKL 146
           GDP H + F +DQ +FLL+RKL
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]
BLAST of Cucsat.G12064.T5 vs. ExPASy Swiss-Prot
Match: Q19826 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis elegans OX=6239 GN=rpb-8 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 4.1e-32
Identity = 64/145 (44.14%), Postives = 101/145 (69.66%), Query Frame = 0 

Query: 1   MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
           MAG++F+D+FKVK V+PDGKK+D+VSR    +E   M +++D+N++IYP+ + +K+ +VL
Sbjct: 1   MAGIIFDDMFKVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKVRLVL 60

Query: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSD-LKVEVYASFGGLQ 120
           + TL  DG       D + +     ++EY+M+GK Y+L D+ +G+D  K+  YASFGGL 
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDTGTDGGKLAAYASFGGLL 120

Query: 121 MLLKGDPIHCAKFRVDQNMFLLLRK 145
           M LKG+ I+   F VD N++LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

[Close]
BLAST of Cucsat.G12064.T5 vs. ExPASy Swiss-Prot
Match: A8XT81 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis briggsae OX=6238 GN=rpb-8 PE=3 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 2.7e-31
Identity = 63/145 (43.45%), Postives = 99/145 (68.28%), Query Frame = 0 

Query: 1   MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
           MAG++F+D+F VK V+PDGKK+D+VSR    +E   M +++D+N++IYP+ + +K  +VL
Sbjct: 1   MAGIIFDDMFSVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKFRLVL 60

Query: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSD-LKVEVYASFGGLQ 120
           + TL  DG       D + +     ++EY+M+GK Y+L D+ SG+D  ++  YASFGGL 
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDSGTDGGQLAAYASFGGLL 120

Query: 121 MLLKGDPIHCAKFRVDQNMFLLLRK 145
           M LKG+ I+   F VD N++LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

[Close]
BLAST of Cucsat.G12064.T5 vs. ExPASy Swiss-Prot
Match: P52434 (DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Homo sapiens OX=9606 GN=POLR2H PE=1 SV=4)

HSP 1 Score: 133.3 bits (334), Expect = 2.3e-30
Identity = 63/148 (42.57%), Postives = 99/148 (66.89%), Query Frame = 0 

Query: 1   MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
           MAG+LF DIF VK ++P+GKK+D+VSR+   SE   M ++LDVN +IYP+D G+K  +V+
Sbjct: 1   MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVI 60

Query: 61  SPTLNYDGS-PVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSD--LKVEVYASFGG 120
           + TL  DG+     Y   + + S AD+FEY+M+GK Y++  + + ++   ++  Y S+GG
Sbjct: 61  ASTLYEDGTLDDGEYNPTDDRPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGG 120

Query: 121 LQMLLKGDPIHCAKFRVDQNMFLLLRKL 146
           L M L+GD  +   F VD  ++LL++KL
Sbjct: 121 LLMRLQGDANNLHGFEVDSRVYLLMKKL 148

[Close]
BLAST of Cucsat.G12064.T5 vs. NCBI nr
Match: XP_004149143.1 (DNA-directed RNA polymerases II, IV and V subunit 8B isoform X1 [Cucumis sativus] >XP_011653128.1 DNA-directed RNA polymerases II, IV and V subunit 8B isoform X1 [Cucumis sativus] >XP_011653129.1 DNA-directed RNA polymerases II, IV and V subunit 8B isoform X1 [Cucumis sativus] >XP_031740209.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like isoform X1 [Cucumis sativus] >XP_031740210.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like isoform X1 [Cucumis sativus] >XP_031740211.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like isoform X1 [Cucumis sativus] >XP_031740212.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like isoform X1 [Cucumis sativus] >XP_031745816.1 DNA-directed RNA polymerases II, IV and V subunit 8B isoform X1 [Cucumis sativus] >KAE8637582.1 hypothetical protein CSA_017859 [Cucumis sativus] >KGN53231.1 hypothetical protein Csa_015226 [Cucumis sativus])

HSP 1 Score: 296 bits (759), Expect = 2.25e-101
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0 

Query: 1   MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
           MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL
Sbjct: 1   MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60

Query: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRKLT 146
           LLKGDPIHCAKFRVDQNMFLLLRKLT
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKLT 146

[Close]
BLAST of Cucsat.G12064.T5 vs. NCBI nr
Match: XP_008453774.1 (PREDICTED: DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo] >KAA0044730.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo var. makuwa] >TYK16730.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo var. makuwa])

HSP 1 Score: 289 bits (739), Expect = 2.53e-98
Identity = 142/146 (97.26%), Postives = 145/146 (99.32%), Query Frame = 0 

Query: 1   MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
           MAGLLFND+FKVKCVNPDGKKYDKVSRI+ARSEKLSM MLLDVNTEIYPIDEGEKLLMVL
Sbjct: 1   MAGLLFNDVFKVKCVNPDGKKYDKVSRIDARSEKLSMSMLLDVNTEIYPIDEGEKLLMVL 60

Query: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRKLT 146
           LLKGDPI+CAKFRVDQNMFLLLRKLT
Sbjct: 121 LLKGDPINCAKFRVDQNMFLLLRKLT 146

[Close]
BLAST of Cucsat.G12064.T5 vs. NCBI nr
Match: XP_022929200.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >XP_022929202.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >XP_022985172.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_023551974.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita pepo subsp. pepo] >KAG6577274.1 DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015358.1 DNA-directed RNA polymerases II, IV and V subunit 8B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 280 bits (715), Expect = 1.12e-94
Identity = 137/145 (94.48%), Postives = 141/145 (97.24%), Query Frame = 0 

Query: 1   MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
           MA LLFND+FKVKCVNPDGKKYDKVSRIEARSEKLSM+MLLDVNTEIYPIDEGEK+LMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDG+PVTSY  QEGKKSLADKFEYIMHGK YKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145
           LLKGDPIHCAKFRVDQNMFLLLRKL
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

[Close]
BLAST of Cucsat.G12064.T5 vs. NCBI nr
Match: XP_022983196.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983203.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983212.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983221.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983229.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima])

HSP 1 Score: 275 bits (704), Expect = 5.51e-93
Identity = 133/146 (91.10%), Postives = 142/146 (97.26%), Query Frame = 0 

Query: 1   MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
           MA +LF+DIFKVKCVNPDGKKYDKVSRIEARSEKLSM+MLLDVNTEIYPIDEG+K+LMVL
Sbjct: 1   MAEVLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDG+PVTSY  QEGKKSLADKFEYIMHGK YKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRKLT 146
           +LKGDP+HCAKFRVDQNMFLLLRKLT
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 146

[Close]
BLAST of Cucsat.G12064.T5 vs. NCBI nr
Match: KAG6600626.1 (DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031262.1 DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 273 bits (699), Expect = 3.19e-92
Identity = 132/146 (90.41%), Postives = 141/146 (96.58%), Query Frame = 0 

Query: 1   MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
           MA LLF+DIFKVKCVNPDGKKYDKVSRIEARSEKLSM+MLLDVNTEIYPIDEG+K+LMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDG+PVT Y  Q+GKKSLADKFEYIMHGK YKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRKLT 146
           +LKGDP+HCAKFRVDQNMFLLLRKLT
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 146

[Close]
BLAST of Cucsat.G12064.T5 vs. ExPASy TrEMBL
Match: A0A0A0KZC2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G031020 PE=3 SV=1)

HSP 1 Score: 296 bits (759), Expect = 1.09e-101
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0 

Query: 1   MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
           MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL
Sbjct: 1   MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60

Query: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRKLT 146
           LLKGDPIHCAKFRVDQNMFLLLRKLT
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKLT 146

[Close]
BLAST of Cucsat.G12064.T5 vs. ExPASy TrEMBL
Match: A0A5A7TNG5 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G005690 PE=3 SV=1)

HSP 1 Score: 289 bits (739), Expect = 1.22e-98
Identity = 142/146 (97.26%), Postives = 145/146 (99.32%), Query Frame = 0 

Query: 1   MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
           MAGLLFND+FKVKCVNPDGKKYDKVSRI+ARSEKLSM MLLDVNTEIYPIDEGEKLLMVL
Sbjct: 1   MAGLLFNDVFKVKCVNPDGKKYDKVSRIDARSEKLSMSMLLDVNTEIYPIDEGEKLLMVL 60

Query: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRKLT 146
           LLKGDPI+CAKFRVDQNMFLLLRKLT
Sbjct: 121 LLKGDPINCAKFRVDQNMFLLLRKLT 146

[Close]
BLAST of Cucsat.G12064.T5 vs. ExPASy TrEMBL
Match: A0A1S3BY97 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo OX=3656 GN=LOC103494404 PE=3 SV=1)

HSP 1 Score: 289 bits (739), Expect = 1.22e-98
Identity = 142/146 (97.26%), Postives = 145/146 (99.32%), Query Frame = 0 

Query: 1   MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
           MAGLLFND+FKVKCVNPDGKKYDKVSRI+ARSEKLSM MLLDVNTEIYPIDEGEKLLMVL
Sbjct: 1   MAGLLFNDVFKVKCVNPDGKKYDKVSRIDARSEKLSMSMLLDVNTEIYPIDEGEKLLMVL 60

Query: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRKLT 146
           LLKGDPI+CAKFRVDQNMFLLLRKLT
Sbjct: 121 LLKGDPINCAKFRVDQNMFLLLRKLT 146

[Close]
BLAST of Cucsat.G12064.T5 vs. ExPASy TrEMBL
Match: A0A6J1ETP8 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata OX=3662 GN=LOC111435865 PE=3 SV=1)

HSP 1 Score: 280 bits (715), Expect = 5.42e-95
Identity = 137/145 (94.48%), Postives = 141/145 (97.24%), Query Frame = 0 

Query: 1   MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
           MA LLFND+FKVKCVNPDGKKYDKVSRIEARSEKLSM+MLLDVNTEIYPIDEGEK+LMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDG+PVTSY  QEGKKSLADKFEYIMHGK YKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145
           LLKGDPIHCAKFRVDQNMFLLLRKL
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

[Close]
BLAST of Cucsat.G12064.T5 vs. ExPASy TrEMBL
Match: A0A6J1J454 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX=3661 GN=LOC111483257 PE=3 SV=1)

HSP 1 Score: 280 bits (715), Expect = 5.42e-95
Identity = 137/145 (94.48%), Postives = 141/145 (97.24%), Query Frame = 0 

Query: 1   MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60
           MA LLFND+FKVKCVNPDGKKYDKVSRIEARSEKLSM+MLLDVNTEIYPIDEGEK+LMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDG+PVTSY  QEGKKSLADKFEYIMHGK YKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145
           LLKGDPIHCAKFRVDQNMFLLLRKL
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

The following BLAST results are available for this feature:
BLAST of Cucsat.G12064.T5 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9M1A84.4e-4257.75DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=... [more]
O810975.8e-4258.45DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702... [more]
Q198264.1e-3244.14Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab... [more]
A8XT812.7e-3143.45Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab... [more]
P524342.3e-3042.57DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Homo sapiens OX=96... [more]
BLAST of Cucsat.G12064.T5 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004149143.12.25e-101100.00DNA-directed RNA polymerases II, IV and V subunit 8B isoform X1 [Cucumis sativus... [more]
XP_008453774.12.53e-9897.26PREDICTED: DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo] ... [more]
XP_022929200.11.12e-9494.48DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >... [more]
XP_022983196.15.51e-9391.10DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP... [more]
KAG6600626.13.19e-9290.41DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyros... [more]
BLAST of Cucsat.G12064.T5 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KZC21.09e-101100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G031020 PE=3 SV=1[more]
A0A5A7TNG51.22e-9897.26DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo var. makuw... [more]
A0A1S3BY971.22e-9897.26DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo OX=3656 GN... [more]
A0A6J1ETP85.42e-9594.48DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata ... [more]
A0A6J1J4545.42e-9594.48DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005570RNA polymerase, Rpb8SMARTSM00658rpol8neucoord: 2..144
e-value: 5.4E-60
score: 215.3
IPR005570RNA polymerase, Rpb8PFAMPF03870RNA_pol_Rpb8coord: 7..144
e-value: 4.1E-50
score: 169.6
IPR005570RNA polymerase, Rpb8PIRSFPIRSF000779RPB8coord: 1..146
e-value: 6.0E-48
score: 160.0
IPR005570RNA polymerase, Rpb8PANTHERPTHR10917DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3coord: 1..145
NoneNo IPR availableGENE3D2.40.50.140coord: 6..146
e-value: 6.7E-54
score: 183.8
NoneNo IPR availablePANTHERPTHR10917:SF1DNA-DIRECTED RNA POLYMERASE I, IIcoord: 1..145
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 3..144

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G12064Cucsat.G12064gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G12064.T5.E1Cucsat.G12064.T5.E1exon
Cucsat.G12064.T5.E2Cucsat.G12064.T5.E2exon
Cucsat.G12064.T5.E3Cucsat.G12064.T5.E3exon
Cucsat.G12064.T5.E4Cucsat.G12064.T5.E4exon
Cucsat.G12064.T5.E5Cucsat.G12064.T5.E5exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G12064.T5.C1Cucsat.G12064.T5.C1CDS
Cucsat.G12064.T5.C2Cucsat.G12064.T5.C2CDS
Cucsat.G12064.T5.C3Cucsat.G12064.T5.C3CDS
Cucsat.G12064.T5.C4Cucsat.G12064.T5.C4CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G12064.T5Cucsat.G12064.T5-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005736 RNA polymerase I complex
cellular_component GO:0005665 RNA polymerase II, core complex
cellular_component GO:0005666 RNA polymerase III complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity