Cp4.1LG11g06210 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG11g06210
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	mediator of RNA polymerase II transcription subunit 22b-like
Location	Cp4.1LG11: 3959093 .. 3962671 (+)
RNA-Seq Expression	Cp4.1LG11g06210
Synteny	Cp4.1LG11g06210

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGCCTCCCTAGGGTTTCAACTGCTTCAATGGTGCTTCTTCTTCCCCGACATTTCTACTTCTCTCTCTTTCCAATTACCTTGCCGCCATTGCAGAGCTGATTCAATTGCCGTTTCCATTTTTATTATTATTATGAACAAAGGCATTGGTGGTGGAACTGGCCCCACCGCTGCGGCTGCTGCGGCGGCAGCTCAGAAACAGAAACTGCTTCTTCAGAGAGTCGAAACCGATATAGCCAACATTGTCGACAATTTCACCCATCTTGTCAACGTCTCAAGGGTAACGCTCCTCTTTCCTTTTCTTTTTTGCTTTTGCTTCATTGTTTTCTCAACTCAATTCTTCTTCTACGACTTGGAATAGGAAATTTGTCATGTAATTCTCTTTGGTTGGTTGTTTTCCTGTTCTTGATTGAACGATCCCTTGTTTGGAATCCATAATTCGAACTTAGCGTTCTCGTTTCCTTAGGTGAACGATCCACCTGTTAGGAATTCACAGGAATCTTTCATGATGGAAATGCGTGCGTCCAGAATGGTAAGATAATGATTCTTCCTTTTTTTGTTTACCTACTGTTTTTGCCAAGCTTATTTTAAGTTAATTTCTGGATAGATAGCTCTCTGTTTCCTTCCTCAATTGAATGATAATCATCTTATATGTTATTTGAAGCCTTGATTCCTAGGGAGTTACTGATTTTTCAATAAAATCAGATGCTTCTGCATCTCTTCTAGTATGTATTTCTTCTCCTTCACCAGCAATTCCGAGCAAATTCTCTTCTTTAATTAACGTCTATGGTCTTCAAATTCGTAACCCTCCGGCGCTACCAATTGGTTCTTCACATAAATTTACTTGAGTTACCTTTTTAGCTTGAGCAGTGAAAGTTATCTCGTGGAATACTAGAGGCCTTGGGGATCGTTCTAAAAGAACAACATTGAAAAACTCATTCAATGTCACTGTCTTGATATGGTGTTAGTTCATGAGACAAAGATTGCTTTCTTTCTTTTTTTTTCTTTTTACTGAAAAGTTATCAAATCTTTATGGTGCTCCAAAGAAATTGGTTGGATTAATGTTGGATCTATTGAGACATCAGGTGGTTTACTGACTTTGCGGGATACTAGTAAGATAAATGTGAGATCCCACATCGGTTGGGGAGGAGAGCATTCTTTATAAGGGTGTGGAAACCTCTCCCTAGCAGATGTGTTTTAAAAACCATGAGGGAAATCTCGGAAGAGAAAGCCCAAAGAGGACAATATCTGCTAGTGTGGGCTTGGGCCGTTACAACAAAAATCTTGGAGTTTCTTAAAGGAGGCTCCACCCTCTCAATTGAAATATTTTTCCAGAACAAGTGAATTTGTTGGGAAACACACATTTGTCTTCGGAATGAATGCGGTCTTTATTCTCTCATACTGTGAAGGAGCCTTGGTGCATTGGTGGCAATTTTTACATAACTCGTTGGGTTCATGAAAGATCACCATTAGGATGAAAAGCCAAAAGTTGGGAGATAATTCATAGCACTCTTTGATCAATAGCTTTTTTTGTTTCGTAGGAATAAGAATAATTATTTGATGATTCAACAGTATAATGACAAGCTCAGATTTTTTTATTGAGCTCTATCCTTTACTCTCTGAAGCTGGACGTTTTGTTTGGGTCTTATCCCACTTTAGATTTTAAATCAGTTGGCTACAAATATCAGTTTGTGAGGTCATCATCAGGGAAGCTCTTTCCATAGATTTTCGTGTTGATGCCTTTGTGGAATTCAGTTGTTTAGATCTTTTTTGGTGAAAGAATTTTGGCCAGCTTTTATTAGTATTGGCTCTTGTTGTCAGCTGTTGCTTTTCGAGGTTTCTCAGCAGTCGAATTTGGTTTGATGGGTCTTGTTTCGTTTCTTGACAATTCTCATTTGATTGTAATTGTTTTAAAATCTTGTTCAATTTTTTCTTTCATTCCCACATAAGATTTTAGGCAATAATAAATTTGGGATCTGTTCTTTTTTGTTCTTATCAGCTCGATATAGATGATAGCATTTCATTATAATCATAATGGCTACTAATTTAGAGTTTTTTACTTGTTTCTAAACAGGTTCAGGCAGCTGATTCTCTGCTTAAGTTGGTTTCAGAACTTAAGCAGACAGCAATCTTTTCTGGATTTGCTTCACTAAACGACCACGTGGAGCAAAGAATCGTGGAATTCAATCAGCAGGCGGAGCAGACCGACCGTGTCCTATCTAAAATTGGTGAAGAAGCTGCCGTAGGGCTTAAGGAGCTTGAATCTCATTATTATTCTTCAACACAGAGGACTAGTCAGAACAGCCTGACATGATTTGTAAGTAGTGAATGTAAATAAGGATGTGATATTGTTGGCTTCTGAACTCAATTTCTCGCTAACATGGATAGTAAAACATAACCGAACTCTTCTAGCCTATGTTCTTAGCATTTTTCTTTGGCTTTTATTGTTTTGGCATGCTAGATATGCAGGAACTAGCAGAAGGGTTTGGTGTCTTTTCTTACTCAGCTTTTTAGAAGAGTAATTAGGATGGGGAAGAAGGGCTTGACAAGGATGTAAAACATTATTTGGGAAATATGAACAATCAGAATATTATTGTTCAAACTAATGTTATGGCTGAGGACTATGGTGGCAAAAATGGCCGAGCTTGTAGAAGCTCAAAATAACAAATAAAAACTCATAGATTCATTGATGAATGTCCCAAATATTACTTAAACCTTGGGTACGTGAGGAAACATATTATTATTGTAACCGTCCAAGTCTACTACTTCTAGATATTGTTCGATTTAGTTTGTTACATGTCACTGTCAGCTTCACAATTTTAAAACGTGTCTGCTAAGTAGAAGTTTTTATATTTTTATAAGGAGAAACTTCAATTGATTTTGGATTTCACAATCTACCCCCTTGGGGGTTCAATGTTCTTGTTTGCACATCGTCCAATGTCTGGCTTTGATGCCATTTGTAACTGCCTAAGCCCACCGTTAGTAGATATTGTTCGCTTTGGTTTCTTATGTATTATCATCAGCCTCACGGTTTTAAAACGTGTCTATTAGGTTGAGTTTTTCACATCTTTATAAAGAATATTTCATTCCCATTTTCAAGGTTGATCTCACAATTATACCTCTTTATAAATATAAGATTTCATAAGAAGAATCCTTCCTATTGGACTAAAATCGTTGGAAATGGTCTAGAATCAGGACTCTGATAACTATTACTTTTTTTTATTTTTTTTTGAACTTTATACGGTTAATTTTGTTTTCGTCATAACAGGTTGTCTAGCAGCCATTCCAGCTGCATTGCTAGTTCAGTCTCCACCAATGGTTTCATCACTTAGCTCTGGTGGTCTTAGTGAGGGCAATCTCCATGTGCCCTAATCTTCTCCATCCTCGTAGCATTAAAATGTGTTAATGTTGGAAAATTGAGATTGTGTAAAGTCATATGTTGTGATATGATGAAGATATTGATGATGAAGAGAATTTTTTGAGAATAAATTTTTGGATTGACCGGACCCGTCTTCAAGTATGAACATTGTTAGACTGTGAAGTATGATGTTTAACTATTTGAGTTAAATGAGTAATGAGTTT

mRNA sequence

AGCCTCCCTAGGGTTTCAACTGCTTCAATGGTGCTTCTTCTTCCCCGACATTTCTACTTCTCTCTCTTTCCAATTACCTTGCCGCCATTGCAGAGCTGATTCAATTGCCGTTTCCATTTTTATTATTATTATGAACAAAGGCATTGGTGGTGGAACTGGCCCCACCGCTGCGGCTGCTGCGGCGGCAGCTCAGAAACAGAAACTGCTTCTTCAGAGAGTCGAAACCGATATAGCCAACATTGTCGACAATTTCACCCATCTTGTCAACGTCTCAAGGGTGAACGATCCACCTGTTAGGAATTCACAGGAATCTTTCATGATGGAAATGCGTGCGTCCAGAATGGTTCAGGCAGCTGATTCTCTGCTTAAGTTGGTTTCAGAACTTAAGCAGACAGCAATCTTTTCTGGATTTGCTTCACTAAACGACCACGTGGAGCAAAGAATCGTGGAATTCAATCAGCAGGCGGAGCAGACCGACCGTGTCCTATCTAAAATTGGTGAAGAAGCTGCCGTAGGGCTTAAGGAGCTTGAATCTCATTATTATTCTTCAACACAGAGGACTAGTCAGAACAGCCTGACATGATTTGTTGTCTAGCAGCCATTCCAGCTGCATTGCTAGTTCAGTCTCCACCAATGGTTTCATCACTTAGCTCTGGTGGTCTTAGTGAGGGCAATCTCCATGTGCCCTAATCTTCTCCATCCTCGTAGCATTAAAATGTGTTAATGTTGGAAAATTGAGATTGTGTAAAGTCATATGTTGTGATATGATGAAGATATTGATGATGAAGAGAATTTTTTGAGAATAAATTTTTGGATTGACCGGACCCGTCTTCAAGTATGAACATTGTTAGACTGTGAAGTATGATGTTTAACTATTTGAGTTAAATGAGTAATGAGTTT

Coding sequence (CDS)

ATGAACAAAGGCATTGGTGGTGGAACTGGCCCCACCGCTGCGGCTGCTGCGGCGGCAGCTCAGAAACAGAAACTGCTTCTTCAGAGAGTCGAAACCGATATAGCCAACATTGTCGACAATTTCACCCATCTTGTCAACGTCTCAAGGGTGAACGATCCACCTGTTAGGAATTCACAGGAATCTTTCATGATGGAAATGCGTGCGTCCAGAATGGTTCAGGCAGCTGATTCTCTGCTTAAGTTGGTTTCAGAACTTAAGCAGACAGCAATCTTTTCTGGATTTGCTTCACTAAACGACCACGTGGAGCAAAGAATCGTGGAATTCAATCAGCAGGCGGAGCAGACCGACCGTGTCCTATCTAAAATTGGTGAAGAAGCTGCCGTAGGGCTTAAGGAGCTTGAATCTCATTATTATTCTTCAACACAGAGGACTAGTCAGAACAGCCTGACATGA

Protein sequence

MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLSKIGEEAAVGLKELESHYYSSTQRTSQNSLT

Homology

[Close]

BLAST of Cp4.1LG11g06210 vs. ExPASy Swiss-Prot
Match: Q8LCH5 (Mediator of RNA polymerase II transcription subunit 22b OS=Arabidopsis thaliana OX=3702 GN=MED22B PE=1 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 3.6e-55
Identity = 112/140 (80.00%), Postives = 130/140 (92.86%), Query Frame = 0


Query: 4   GIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFM 63
           G GGG+GPTAAAAAAA QKQK LLQRVETDI ++VDNFT +VNVSRV+DPPV+NSQE++M
Sbjct: 10  GSGGGSGPTAAAAAAALQKQKALLQRVETDITSVVDNFTQIVNVSRVSDPPVKNSQETYM 69

Query: 64  MEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLSKIG 123
           MEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRI EF+Q+AE+T+R+L++I 
Sbjct: 70  MEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIEEFDQEAEKTNRLLARIA 129

Query: 124 EEAAVGLKELESHYYSSTQR 144
           ++A+  LKELESHYYSS QR
Sbjct: 130 DDASANLKELESHYYSSAQR 149

[Close]

BLAST of Cp4.1LG11g06210 vs. ExPASy Swiss-Prot
Match: Q9SA42 (Mediator of RNA polymerase II transcription subunit 22a OS=Arabidopsis thaliana OX=3702 GN=MED22A PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 5.2e-54
Identity = 110/147 (74.83%), Postives = 134/147 (91.16%), Query Frame = 0


Query: 1   MNK--GIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNS 60
           MNK  G GGG+GPTAAAAAAA QKQK LLQRV+TDI ++VDNF  +VNV+RV+DPP++NS
Sbjct: 2   MNKGGGSGGGSGPTAAAAAAALQKQKALLQRVDTDITSVVDNFNQIVNVARVSDPPMKNS 61

Query: 61  QESFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRV 120
           QE++MMEMRASR+VQAADSLLKLVSELKQTAIFSGFASLNDHVEQRI EF+Q+AE+T+R+
Sbjct: 62  QEAYMMEMRASRLVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIAEFDQEAEKTNRL 121

Query: 121 LSKIGEEAAVGLKELESHYYSSTQRTS 146
           L++I ++A+  LKELESHYYSS QR++
Sbjct: 122 LARIADDASASLKELESHYYSSAQRST 148

[Close]

BLAST of Cp4.1LG11g06210 vs. ExPASy Swiss-Prot
Match: Q54XB0 (Putative mediator of RNA polymerase II transcription subunit 22 OS=Dictyostelium discoideum OX=44689 GN=med22 PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.8e-15
Identity = 45/143 (31.47%), Postives = 88/143 (61.54%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MN G G  + P         + Q+   +++++ I  +++N+ +++ +S+VND P++N+ E
Sbjct: 1   MNTG-GNNSLPPQTRGEVLFRDQQFFQKQIDSKIMQLLENYHNIIKISKVND-PLKNASE 60

Query: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLS 120
            + ME R S M+ A + LLK++ ELKQ  I + F+++ + V  + + F+++ E+T++ + 
Sbjct: 61  IYEMETRTSNMLNAGEGLLKIIEELKQNLILNDFSTMAEEVRIQNLVFHKENERTNKSIK 120

Query: 121 KIGEEAAVGLKELESHYYSSTQR 144
            I EE +  LKELE  YY+S+ +
Sbjct: 121 LISEELSRSLKELEDEYYNSSYK 141

[Close]

BLAST of Cp4.1LG11g06210 vs. ExPASy Swiss-Prot
Match: Q62276 (Mediator of RNA polymerase II transcription subunit 22 OS=Mus musculus OX=10090 GN=Med22 PE=1 SV=2)

HSP 1 Score: 83.2 bits (204), Expect = 2.8e-15
Identity = 49/137 (35.77%), Postives = 83/137 (60.58%), Query Frame = 0


Query: 14  AAAAAAAQKQKLLLQ----RVETDIANIVDNFTHLVNVSRVNDP-----PVRNSQESFMM 73
           A   A  Q ++ LLQ    R++ DI +I+DNFT ++  +++ D        +  Q+++ M
Sbjct: 2   AQQRALPQSKETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEM 61

Query: 74  EMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLSKIGE 133
            +RA+ +V+A +SL+KLVS+LKQ  I + F S+N+ ++QR  +     E+ DR L  + +
Sbjct: 62  HVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRALQEECDRKLITLRD 121

Query: 134 EAAVGLKELESHYYSST 142
           E ++ L ELE  YYSS+
Sbjct: 122 EVSIDLYELEEEYYSSS 138

[Close]

BLAST of Cp4.1LG11g06210 vs. ExPASy Swiss-Prot
Match: A0JPN6 (Mediator of RNA polymerase II transcription subunit 22 OS=Rattus norvegicus OX=10116 GN=Med22 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.8e-15
Identity = 49/137 (35.77%), Postives = 83/137 (60.58%), Query Frame = 0


Query: 14  AAAAAAAQKQKLLLQ----RVETDIANIVDNFTHLVNVSRVNDP-----PVRNSQESFMM 73
           A   A  Q ++ LLQ    R++ DI +I+DNFT ++  +++ D        +  Q+++ M
Sbjct: 2   AQQRALPQSKETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEM 61

Query: 74  EMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLSKIGE 133
            +RA+ +V+A +SL+KLVS+LKQ  I + F S+N+ ++QR  +     E+ DR L  + +
Sbjct: 62  HVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRALQEECDRKLITLRD 121

Query: 134 EAAVGLKELESHYYSST 142
           E ++ L ELE  YYSS+
Sbjct: 122 EVSIDLYELEEEYYSSS 138

[Close]

BLAST of Cp4.1LG11g06210 vs. NCBI nr
Match: XP_023545554.1 (mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita pepo subsp. pepo] >XP_023545555.1 mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita pepo subsp. pepo] >XP_023545556.1 mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 279 bits (713), Expect = 3.15e-94
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLS 120
           SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLS
Sbjct: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLS 120

Query: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 150
           KIGEEAAVGLKELESHYYSSTQRTSQNSLT
Sbjct: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 150

[Close]

BLAST of Cp4.1LG11g06210 vs. NCBI nr
Match: KAG7029838.1 (Mediator of RNA polymerase II transcription subunit 22b [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 278 bits (712), Expect = 4.48e-94
Identity = 149/150 (99.33%), Postives = 150/150 (100.00%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLS 120
           SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDR+LS
Sbjct: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRILS 120

Query: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 150
           KIGEEAAVGLKELESHYYSSTQRTSQNSLT
Sbjct: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 150

[Close]

BLAST of Cp4.1LG11g06210 vs. NCBI nr
Match: XP_022929626.1 (mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita moschata] >XP_022929627.1 mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita moschata] >XP_022929628.1 mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita moschata])

HSP 1 Score: 276 bits (705), Expect = 5.23e-93
Identity = 147/150 (98.00%), Postives = 149/150 (99.33%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLS 120
           SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDR++S
Sbjct: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRIIS 120

Query: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 150
           KIGEEAAVGLKELESHYYSSTQRTSQN LT
Sbjct: 121 KIGEEAAVGLKELESHYYSSTQRTSQNRLT 150

[Close]

BLAST of Cp4.1LG11g06210 vs. NCBI nr
Match: XP_022996928.1 (mediator of RNA polymerase II transcription subunit 22b-like isoform X1 [Cucurbita maxima] >XP_022996929.1 mediator of RNA polymerase II transcription subunit 22b-like isoform X1 [Cucurbita maxima] >XP_022996930.1 mediator of RNA polymerase II transcription subunit 22b-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 273 bits (697), Expect = 8.68e-92
Identity = 146/150 (97.33%), Postives = 147/150 (98.00%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLS 120
           SFMMEMRASRMVQAADSLLKLVSELKQ AIFSGFASLNDHVEQRIVEFN QAEQTDR+LS
Sbjct: 61  SFMMEMRASRMVQAADSLLKLVSELKQAAIFSGFASLNDHVEQRIVEFNMQAEQTDRILS 120

Query: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 150
           KIGEEAAVGLKELESHYYSSTQRT QNSLT
Sbjct: 121 KIGEEAAVGLKELESHYYSSTQRTCQNSLT 150

[Close]

BLAST of Cp4.1LG11g06210 vs. NCBI nr
Match: XP_004145443.1 (mediator of RNA polymerase II transcription subunit 22b [Cucumis sativus] >KGN66872.1 hypothetical protein Csa_007213 [Cucumis sativus])

HSP 1 Score: 269 bits (687), Expect = 2.91e-90
Identity = 143/150 (95.33%), Postives = 147/150 (98.00%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLS 120
           SFMMEMRA+RMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQA+QTDR+LS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120

Query: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 150
           +IGEEAA  LKELESHYYSSTQRT QNSLT
Sbjct: 121 RIGEEAAANLKELESHYYSSTQRTDQNSLT 150

[Close]

BLAST of Cp4.1LG11g06210 vs. ExPASy TrEMBL
Match: A0A6J1EN95 (mediator of RNA polymerase II transcription subunit 22b-like OS=Cucurbita moschata OX=3662 GN=LOC111436153 PE=4 SV=1)

HSP 1 Score: 276 bits (705), Expect = 2.53e-93
Identity = 147/150 (98.00%), Postives = 149/150 (99.33%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLS 120
           SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDR++S
Sbjct: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRIIS 120

Query: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 150
           KIGEEAAVGLKELESHYYSSTQRTSQN LT
Sbjct: 121 KIGEEAAVGLKELESHYYSSTQRTSQNRLT 150

[Close]

BLAST of Cp4.1LG11g06210 vs. ExPASy TrEMBL
Match: A0A6J1K851 (mediator of RNA polymerase II transcription subunit 22b-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492015 PE=4 SV=1)

HSP 1 Score: 273 bits (697), Expect = 4.20e-92
Identity = 146/150 (97.33%), Postives = 147/150 (98.00%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLS 120
           SFMMEMRASRMVQAADSLLKLVSELKQ AIFSGFASLNDHVEQRIVEFN QAEQTDR+LS
Sbjct: 61  SFMMEMRASRMVQAADSLLKLVSELKQAAIFSGFASLNDHVEQRIVEFNMQAEQTDRILS 120

Query: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 150
           KIGEEAAVGLKELESHYYSSTQRT QNSLT
Sbjct: 121 KIGEEAAVGLKELESHYYSSTQRTCQNSLT 150

[Close]

BLAST of Cp4.1LG11g06210 vs. ExPASy TrEMBL
Match: A0A0A0M174 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G701970 PE=4 SV=1)

HSP 1 Score: 269 bits (687), Expect = 1.41e-90
Identity = 143/150 (95.33%), Postives = 147/150 (98.00%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLS 120
           SFMMEMRA+RMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQA+QTDR+LS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120

Query: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 150
           +IGEEAA  LKELESHYYSSTQRT QNSLT
Sbjct: 121 RIGEEAAANLKELESHYYSSTQRTDQNSLT 150

[Close]

BLAST of Cp4.1LG11g06210 vs. ExPASy TrEMBL
Match: A0A1S3C993 (mediator of RNA polymerase II transcription subunit 22b-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498265 PE=4 SV=1)

HSP 1 Score: 266 bits (680), Expect = 1.64e-89
Identity = 141/150 (94.00%), Postives = 147/150 (98.00%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLS 120
           SFMMEMRA+RMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQA+QT+R+LS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTERLLS 120

Query: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 150
           +IGEEAA  LKELESHYYSSTQRT QN+LT
Sbjct: 121 RIGEEAAANLKELESHYYSSTQRTDQNNLT 150

[Close]

BLAST of Cp4.1LG11g06210 vs. ExPASy TrEMBL
Match: A0A6J1DR20 (mediator of RNA polymerase II transcription subunit 22b-like OS=Momordica charantia OX=3673 GN=LOC111022360 PE=4 SV=1)

HSP 1 Score: 266 bits (679), Expect = 2.33e-89
Identity = 141/150 (94.00%), Postives = 146/150 (97.33%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKG+GGG GPTAAAAAAAAQKQK+LLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGVGGGNGPTAAAAAAAAQKQKVLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLS 120
           SFMMEMRA+RMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDR+LS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRLLS 120

Query: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 150
           +IGEEAA  LKELESHYYSSTQRTSQN LT
Sbjct: 121 RIGEEAATSLKELESHYYSSTQRTSQNILT 150

[Close]

BLAST of Cp4.1LG11g06210 vs. TAIR 10
Match: AT1G07950.1 (Surfeit locus protein 5 subunit 22 of Mediator complex )

HSP 1 Score: 215.7 bits (548), Expect = 2.5e-56
Identity = 112/140 (80.00%), Postives = 130/140 (92.86%), Query Frame = 0


Query: 4   GIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFM 63
           G GGG+GPTAAAAAAA QKQK LLQRVETDI ++VDNFT +VNVSRV+DPPV+NSQE++M
Sbjct: 10  GSGGGSGPTAAAAAAALQKQKALLQRVETDITSVVDNFTQIVNVSRVSDPPVKNSQETYM 69

Query: 64  MEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRVLSKIG 123
           MEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRI EF+Q+AE+T+R+L++I 
Sbjct: 70  MEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIEEFDQEAEKTNRLLARIA 129

Query: 124 EEAAVGLKELESHYYSSTQR 144
           ++A+  LKELESHYYSS QR
Sbjct: 130 DDASANLKELESHYYSSAQR 149

[Close]

BLAST of Cp4.1LG11g06210 vs. TAIR 10
Match: AT1G16430.1 (Surfeit locus protein 5 subunit 22 of Mediator complex )

HSP 1 Score: 211.8 bits (538), Expect = 3.7e-55
Identity = 110/147 (74.83%), Postives = 134/147 (91.16%), Query Frame = 0


Query: 1   MNK--GIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNS 60
           MNK  G GGG+GPTAAAAAAA QKQK LLQRV+TDI ++VDNF  +VNV+RV+DPP++NS
Sbjct: 2   MNKGGGSGGGSGPTAAAAAAALQKQKALLQRVDTDITSVVDNFNQIVNVARVSDPPMKNS 61

Query: 61  QESFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRV 120
           QE++MMEMRASR+VQAADSLLKLVSELKQTAIFSGFASLNDHVEQRI EF+Q+AE+T+R+
Sbjct: 62  QEAYMMEMRASRLVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIAEFDQEAEKTNRL 121

Query: 121 LSKIGEEAAVGLKELESHYYSSTQRTS 146
           L++I ++A+  LKELESHYYSS QR++
Sbjct: 122 LARIADDASASLKELESHYYSSAQRST 148

The following BLAST results are available for this feature:

BLAST of Cp4.1LG11g06210 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LCH5	3.6e-55	80.00	Mediator of RNA polymerase II transcription subunit 22b OS=Arabidopsis thaliana ...	[more]
Q9SA42	5.2e-54	74.83	Mediator of RNA polymerase II transcription subunit 22a OS=Arabidopsis thaliana ...	[more]
Q54XB0	2.8e-15	31.47	Putative mediator of RNA polymerase II transcription subunit 22 OS=Dictyostelium...	[more]
Q62276	2.8e-15	35.77	Mediator of RNA polymerase II transcription subunit 22 OS=Mus musculus OX=10090 ...	[more]
A0JPN6	2.8e-15	35.77	Mediator of RNA polymerase II transcription subunit 22 OS=Rattus norvegicus OX=1...	[more]

BLAST of Cp4.1LG11g06210 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023545554.1	3.15e-94	100.00	mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita pepo sub...	[more]
KAG7029838.1	4.48e-94	99.33	Mediator of RNA polymerase II transcription subunit 22b [Cucurbita argyrosperma ...	[more]
XP_022929626.1	5.23e-93	98.00	mediator of RNA polymerase II transcription subunit 22b-like [Cucurbita moschata...	[more]
XP_022996928.1	8.68e-92	97.33	mediator of RNA polymerase II transcription subunit 22b-like isoform X1 [Cucurbi...	[more]
XP_004145443.1	2.91e-90	95.33	mediator of RNA polymerase II transcription subunit 22b [Cucumis sativus] >KGN66...	[more]

BLAST of Cp4.1LG11g06210 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EN95	2.53e-93	98.00	mediator of RNA polymerase II transcription subunit 22b-like OS=Cucurbita moscha...	[more]
A0A6J1K851	4.20e-92	97.33	mediator of RNA polymerase II transcription subunit 22b-like isoform X1 OS=Cucur...	[more]
A0A0A0M174	1.41e-90	95.33	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G701970 PE=4 SV=1	[more]
A0A1S3C993	1.64e-89	94.00	mediator of RNA polymerase II transcription subunit 22b-like isoform X2 OS=Cucum...	[more]
A0A6J1DR20	2.33e-89	94.00	mediator of RNA polymerase II transcription subunit 22b-like OS=Momordica charan...	[more]

BLAST of Cp4.1LG11g06210 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G07950.1	2.5e-56	80.00	Surfeit locus protein 5 subunit 22 of Mediator complex	[more]
AT1G16430.1	3.7e-55	74.83	Surfeit locus protein 5 subunit 22 of Mediator complex	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009332	Mediator of RNA polymerase II transcription subunit 22	PFAM	PF06179	Med22	coord: 28..126 e-value: 5.0E-28 score: 97.4
IPR009332	Mediator of RNA polymerase II transcription subunit 22	PANTHER	PTHR12434	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 22	coord: 1..146

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG11g06210.1	Cp4.1LG11g06210.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0016592	mediator complex
molecular_function	GO:0003712	transcription coregulator activity