CmaCh16G009840 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G009840
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Subtilisin-like protease
Location	Cma_Chr16: 7576425 .. 7577457 (-)
RNA-Seq Expression	CmaCh16G009840
Synteny	CmaCh16G009840

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGGAATTCATTCAAACCCAATTTGGAATATTGCAAGATTTGGTGAATCTACAATCATAGGCAACCTTGACACAGGTTGGTCTTGTTTTCCTCTTGTAAGTTCTTGGACATTCGACAGCTTTGATAATAATTTGGATACCTCTTCTTCTTGTAATTGGGATCCATACAGGTGTTTGGCCAGAATCAACGAGTTTTAGTGATGATGGATATGGATCTATCCCAAAAAGATGGAAAGAAAGTTGTGAAGGTGACTCCAATTTTCGTTGTAACAGGTCCCTTGAATCAAATCTCTTATTGTTACTTCACTTCTATTTAATTCTTTTACGCTAAGAAACATTGACTAATATGTTAAGCACAAATTAGAAACTCTTGTGAGCAGATATACACTCAACATTAGTGTTATAAAGTAAAGATTAATCTAACATATAAATGCATAAATTCATCATATTTTGAATTTTCTTCTTATATAAAATTGATAATAGAACTGTCTTGATTGTGTTTGTGTTGTGTTCAAGCAAATTTGATATCGATGTAAAATTGAAGCTTTTAAATGCAGAAAATTGATTGGGGCACGGTATTTCAACAAAGGGTATGCATCGATGGTGGGGCCTCTCAACTTAATGGCAAGGGACGATCAAGGGCATAGAACACACACTTTATTCACAGTTGCAGGCAATTTCGTTTCAAAAGCAAATATTTTTGGGAATGGTAATGGTACTCCAATTTTCGTTTCAAAAGCAAATATTTTTGGGAATGGTAATGTTGGCCTCCTGTGGCACTTGGTGAGTGCTTTGATTCCGACATTCTGGTTGGCTTTGAAGCTGCCATTAGTGATGCCATTAGTGATGGAGTTGACGTTCTGTCTATTTCACTCGGTGGAAGTCCTCTAGAATTTCATGATGATCCAATAGCTATAGCATCCTGCCATGCAGTGAAGAACGACATCACTGTCGTTTGTTCTGCTGGAAACTCGGGACCATCTCCAGGCACTGTCAAATGTCGCACCATGGTTGATAACCGTGGGAGCTAG

mRNA sequence

ATGATGGAATTCATTCAAACCCAATTTGGAATATTGCAAGATTTGGTGTTTGGCCAGAATCAACGAGTTTTAGTGATGATGGATATGGATCTATCCCAAAAAGATGGAAAGAAAGTTGTGAAGGTGACTCCAATTTTCGTTGTAACAGCTGCCATTAGTGATGCCATTAGTGATGGAGTTGACGTTCTGTCTATTTCACTCGGTGGAAGTCCTCTAGAATTTCATGATGATCCAATAGCTATAGCATCCTGCCATGCAGTGAAGAACGACATCACTGTCGTTTGTTCTGCTGGAAACTCGGGACCATCTCCAGGCACTGTCAAATGTCGCACCATGGTTGATAACCGTGGGAGCTAG

Coding sequence (CDS)

ATGATGGAATTCATTCAAACCCAATTTGGAATATTGCAAGATTTGGTGTTTGGCCAGAATCAACGAGTTTTAGTGATGATGGATATGGATCTATCCCAAAAAGATGGAAAGAAAGTTGTGAAGGTGACTCCAATTTTCGTTGTAACAGCTGCCATTAGTGATGCCATTAGTGATGGAGTTGACGTTCTGTCTATTTCACTCGGTGGAAGTCCTCTAGAATTTCATGATGATCCAATAGCTATAGCATCCTGCCATGCAGTGAAGAACGACATCACTGTCGTTTGTTCTGCTGGAAACTCGGGACCATCTCCAGGCACTGTCAAATGTCGCACCATGGTTGATAACCGTGGGAGCTAG

Protein sequence

MMEFIQTQFGILQDLVFGQNQRVLVMMDMDLSQKDGKKVVKVTPIFVVTAAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTVKCRTMVDNRGS

Homology

[Close]

BLAST of CmaCh16G009840 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 7.8e-13
Identity = 39/60 (65.00%), Postives = 44/60 (73.33%), Query Frame = 0


Query: 48  VTAAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTV 107
           + AAI  AI DGVDVLS S+GG   ++  D IAI S HAVKN +TVVCSAGNSGP  GTV
Sbjct: 285 ILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTV 344

[Close]

BLAST of CmaCh16G009840 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 8.6e-12
Identity = 36/60 (60.00%), Postives = 41/60 (68.33%), Query Frame = 0


Query: 48  VTAAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTV 107
           V AA   AI DG DV+S+SLGG P  F +D +AI S HA K  I VVCSAGNSGP+  TV
Sbjct: 278 VLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTV 337

[Close]

BLAST of CmaCh16G009840 vs. ExPASy Swiss-Prot
Match: Q9LZS6 (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 2.5e-11
Identity = 34/58 (58.62%), Postives = 43/58 (74.14%), Query Frame = 0


Query: 50  AAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTV 108
           AA  +AISDGVD++SIS+GG+ L F +DPIAI + HA+K  I   CSAGN+GP   TV
Sbjct: 263 AAFDEAISDGVDIISISIGGASLPFFEDPIAIGAFHAMKRGILTTCSAGNNGPGLFTV 320

[Close]

BLAST of CmaCh16G009840 vs. ExPASy Swiss-Prot
Match: F4HSQ2 (Subtilisin-like protease SBT5.1 OS=Arabidopsis thaliana OX=3702 GN=SBT5.1 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 7.3e-11
Identity = 35/60 (58.33%), Postives = 44/60 (73.33%), Query Frame = 0


Query: 48  VTAAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTV 107
           + AA  DAI+DGVDV+SIS+G  P    +DP++I S HAV+  ITVVCS GNSGPS  +V
Sbjct: 266 ILAAFDDAIADGVDVISISMGLWPDNLLEDPLSIGSFHAVERGITVVCSVGNSGPSSQSV 325

[Close]

BLAST of CmaCh16G009840 vs. ExPASy Swiss-Prot
Match: Q9LNU1 (CO(2)-response secreted protease OS=Arabidopsis thaliana OX=3702 GN=CRSP PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.2e-10
Identity = 35/63 (55.56%), Postives = 45/63 (71.43%), Query Frame = 0


Query: 48  VTAAISDAISDGVDVLSISLGG---SPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSP 107
           + AA  DAI+DGVDVLS+SLG    + ++ + DPIAI + HAV+  I V+CSAGN GP  
Sbjct: 261 ILAAFDDAIADGVDVLSLSLGAPAYARIDLNTDPIAIGAFHAVEQGILVICSAGNDGPDG 320

[Close]

BLAST of CmaCh16G009840 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 74.7 bits (182), Expect = 5.5e-14
Identity = 39/60 (65.00%), Postives = 44/60 (73.33%), Query Frame = 0


Query: 48  VTAAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTV 107
           + AAI  AI DGVDVLS S+GG   ++  D IAI S HAVKN +TVVCSAGNSGP  GTV
Sbjct: 285 ILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTV 344

[Close]

BLAST of CmaCh16G009840 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 71.2 bits (173), Expect = 6.1e-13
Identity = 36/60 (60.00%), Postives = 41/60 (68.33%), Query Frame = 0


Query: 48  VTAAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTV 107
           V AA   AI DG DV+S+SLGG P  F +D +AI S HA K  I VVCSAGNSGP+  TV
Sbjct: 278 VLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTV 337

[Close]

BLAST of CmaCh16G009840 vs. TAIR 10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 69.7 bits (169), Expect = 1.8e-12
Identity = 34/58 (58.62%), Postives = 43/58 (74.14%), Query Frame = 0


Query: 50  AAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTV 108
           AA  +AISDGVD++SIS+GG+ L F +DPIAI + HA+K  I   CSAGN+GP   TV
Sbjct: 263 AAFDEAISDGVDIISISIGGASLPFFEDPIAIGAFHAMKRGILTTCSAGNNGPGLFTV 320

[Close]

BLAST of CmaCh16G009840 vs. TAIR 10
Match: AT1G20150.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 68.2 bits (165), Expect = 5.2e-12
Identity = 35/60 (58.33%), Postives = 44/60 (73.33%), Query Frame = 0


Query: 48  VTAAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTV 107
           + AA  DAI+DGVDV+SIS+G  P    +DP++I S HAV+  ITVVCS GNSGPS  +V
Sbjct: 266 ILAAFDDAIADGVDVISISMGLWPDNLLEDPLSIGSFHAVERGITVVCSVGNSGPSSQSV 325

[Close]

BLAST of CmaCh16G009840 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 67.4 bits (163), Expect = 8.8e-12
Identity = 37/60 (61.67%), Postives = 44/60 (73.33%), Query Frame = 0


Query: 48  VTAAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTV 107
           V AAI  AI DGVDV+S+SLGGS   F+ DPIAIA   A++ +I V CSAGNSGP+  TV
Sbjct: 276 VIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGFGAMQKNIFVSCSAGNSGPTASTV 335

The following BLAST results are available for this feature:

BLAST of CmaCh16G009840 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JXC5	7.8e-13	65.00	Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S...	[more]
Q9ZSP5	8.6e-12	60.00	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=...	[more]
Q9LZS6	2.5e-11	58.62	Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3...	[more]
F4HSQ2	7.3e-11	58.33	Subtilisin-like protease SBT5.1 OS=Arabidopsis thaliana OX=3702 GN=SBT5.1 PE=3 S...	[more]
Q9LNU1	1.2e-10	55.56	CO(2)-response secreted protease OS=Arabidopsis thaliana OX=3702 GN=CRSP PE=2 SV...	[more]

BLAST of CmaCh16G009840 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59810.1	5.5e-14	65.00	Subtilase family protein	[more]
AT2G04160.1	6.1e-13	60.00	Subtilisin-like serine endopeptidase family protein	[more]
AT5G03620.1	1.8e-12	58.62	Subtilisin-like serine endopeptidase family protein	[more]
AT1G20150.1	5.2e-12	58.33	Subtilisin-like serine endopeptidase family protein	[more]
AT1G01900.1	8.8e-12	61.67	subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 48..108 e-value: 2.6E-21 score: 77.9
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 45..110
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 48..109 e-value: 3.8E-7 score: 29.7
None	No IPR available	PANTHER	PTHR43806	PEPTIDASE S8	coord: 49..108
None	No IPR available	PANTHER	PTHR43806:SF34	SUBTILISIN-LIKE PROTEASE SBT5.4	coord: 49..108

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G009840.1	CmaCh16G009840.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0009610	response to symbiotic fungus
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity