CmaCh16G009840.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G009840.1
TypemRNA
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionSubtilisin-like protease
LocationCma_Chr16: 7576425 .. 7577457 (-)
Sequence length357
RNA-Seq ExpressionCmaCh16G009840.1
SyntenyCmaCh16G009840.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGGAATTCATTCAAACCCAATTTGGAATATTGCAAGATTTGGTGAATCTACAATCATAGGCAACCTTGACACAGGTTGGTCTTGTTTTCCTCTTGTAAGTTCTTGGACATTCGACAGCTTTGATAATAATTTGGATACCTCTTCTTCTTGTAATTGGGATCCATACAGGTGTTTGGCCAGAATCAACGAGTTTTAGTGATGATGGATATGGATCTATCCCAAAAAGATGGAAAGAAAGTTGTGAAGGTGACTCCAATTTTCGTTGTAACAGGTCCCTTGAATCAAATCTCTTATTGTTACTTCACTTCTATTTAATTCTTTTACGCTAAGAAACATTGACTAATATGTTAAGCACAAATTAGAAACTCTTGTGAGCAGATATACACTCAACATTAGTGTTATAAAGTAAAGATTAATCTAACATATAAATGCATAAATTCATCATATTTTGAATTTTCTTCTTATATAAAATTGATAATAGAACTGTCTTGATTGTGTTTGTGTTGTGTTCAAGCAAATTTGATATCGATGTAAAATTGAAGCTTTTAAATGCAGAAAATTGATTGGGGCACGGTATTTCAACAAAGGGTATGCATCGATGGTGGGGCCTCTCAACTTAATGGCAAGGGACGATCAAGGGCATAGAACACACACTTTATTCACAGTTGCAGGCAATTTCGTTTCAAAAGCAAATATTTTTGGGAATGGTAATGGTACTCCAATTTTCGTTTCAAAAGCAAATATTTTTGGGAATGGTAATGTTGGCCTCCTGTGGCACTTGGTGAGTGCTTTGATTCCGACATTCTGGTTGGCTTTGAAGCTGCCATTAGTGATGCCATTAGTGATGGAGTTGACGTTCTGTCTATTTCACTCGGTGGAAGTCCTCTAGAATTTCATGATGATCCAATAGCTATAGCATCCTGCCATGCAGTGAAGAACGACATCACTGTCGTTTGTTCTGCTGGAAACTCGGGACCATCTCCAGGCACTGTCAAATGTCGCACCATGGTTGATAACCGTGGGAGCTAG

mRNA sequence

ATGATGGAATTCATTCAAACCCAATTTGGAATATTGCAAGATTTGGTGTTTGGCCAGAATCAACGAGTTTTAGTGATGATGGATATGGATCTATCCCAAAAAGATGGAAAGAAAGTTGTGAAGGTGACTCCAATTTTCGTTGTAACAGCTGCCATTAGTGATGCCATTAGTGATGGAGTTGACGTTCTGTCTATTTCACTCGGTGGAAGTCCTCTAGAATTTCATGATGATCCAATAGCTATAGCATCCTGCCATGCAGTGAAGAACGACATCACTGTCGTTTGTTCTGCTGGAAACTCGGGACCATCTCCAGGCACTGTCAAATGTCGCACCATGGTTGATAACCGTGGGAGCTAG

Coding sequence (CDS)

ATGATGGAATTCATTCAAACCCAATTTGGAATATTGCAAGATTTGGTGTTTGGCCAGAATCAACGAGTTTTAGTGATGATGGATATGGATCTATCCCAAAAAGATGGAAAGAAAGTTGTGAAGGTGACTCCAATTTTCGTTGTAACAGCTGCCATTAGTGATGCCATTAGTGATGGAGTTGACGTTCTGTCTATTTCACTCGGTGGAAGTCCTCTAGAATTTCATGATGATCCAATAGCTATAGCATCCTGCCATGCAGTGAAGAACGACATCACTGTCGTTTGTTCTGCTGGAAACTCGGGACCATCTCCAGGCACTGTCAAATGTCGCACCATGGTTGATAACCGTGGGAGCTAG

Protein sequence

MMEFIQTQFGILQDLVFGQNQRVLVMMDMDLSQKDGKKVVKVTPIFVVTAAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTVKCRTMVDNRGS
Homology
[Close]
BLAST of CmaCh16G009840.1 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 7.8e-13
Identity = 39/60 (65.00%), Postives = 44/60 (73.33%), Query Frame = 0 

Query: 48  VTAAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTV 107
           + AAI  AI DGVDVLS S+GG   ++  D IAI S HAVKN +TVVCSAGNSGP  GTV
Sbjct: 285 ILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTV 344

[Close]
BLAST of CmaCh16G009840.1 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 8.6e-12
Identity = 36/60 (60.00%), Postives = 41/60 (68.33%), Query Frame = 0 

Query: 48  VTAAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTV 107
           V AA   AI DG DV+S+SLGG P  F +D +AI S HA K  I VVCSAGNSGP+  TV
Sbjct: 278 VLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTV 337

[Close]
BLAST of CmaCh16G009840.1 vs. ExPASy Swiss-Prot
Match: Q9LZS6 (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 2.5e-11
Identity = 34/58 (58.62%), Postives = 43/58 (74.14%), Query Frame = 0 

Query: 50  AAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTV 108
           AA  +AISDGVD++SIS+GG+ L F +DPIAI + HA+K  I   CSAGN+GP   TV
Sbjct: 263 AAFDEAISDGVDIISISIGGASLPFFEDPIAIGAFHAMKRGILTTCSAGNNGPGLFTV 320

[Close]
BLAST of CmaCh16G009840.1 vs. ExPASy Swiss-Prot
Match: F4HSQ2 (Subtilisin-like protease SBT5.1 OS=Arabidopsis thaliana OX=3702 GN=SBT5.1 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 7.3e-11
Identity = 35/60 (58.33%), Postives = 44/60 (73.33%), Query Frame = 0 

Query: 48  VTAAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTV 107
           + AA  DAI+DGVDV+SIS+G  P    +DP++I S HAV+  ITVVCS GNSGPS  +V
Sbjct: 266 ILAAFDDAIADGVDVISISMGLWPDNLLEDPLSIGSFHAVERGITVVCSVGNSGPSSQSV 325

[Close]
BLAST of CmaCh16G009840.1 vs. ExPASy Swiss-Prot
Match: Q9LNU1 (CO(2)-response secreted protease OS=Arabidopsis thaliana OX=3702 GN=CRSP PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.2e-10
Identity = 35/63 (55.56%), Postives = 45/63 (71.43%), Query Frame = 0 

Query: 48  VTAAISDAISDGVDVLSISLGG---SPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSP 107
           + AA  DAI+DGVDVLS+SLG    + ++ + DPIAI + HAV+  I V+CSAGN GP  
Sbjct: 261 ILAAFDDAIADGVDVLSLSLGAPAYARIDLNTDPIAIGAFHAVEQGILVICSAGNDGPDG 320

[Close]
BLAST of CmaCh16G009840.1 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 74.7 bits (182), Expect = 5.5e-14
Identity = 39/60 (65.00%), Postives = 44/60 (73.33%), Query Frame = 0 

Query: 48  VTAAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTV 107
           + AAI  AI DGVDVLS S+GG   ++  D IAI S HAVKN +TVVCSAGNSGP  GTV
Sbjct: 285 ILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTV 344

[Close]
BLAST of CmaCh16G009840.1 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 71.2 bits (173), Expect = 6.1e-13
Identity = 36/60 (60.00%), Postives = 41/60 (68.33%), Query Frame = 0 

Query: 48  VTAAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTV 107
           V AA   AI DG DV+S+SLGG P  F +D +AI S HA K  I VVCSAGNSGP+  TV
Sbjct: 278 VLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTV 337

[Close]
BLAST of CmaCh16G009840.1 vs. TAIR 10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 69.7 bits (169), Expect = 1.8e-12
Identity = 34/58 (58.62%), Postives = 43/58 (74.14%), Query Frame = 0 

Query: 50  AAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTV 108
           AA  +AISDGVD++SIS+GG+ L F +DPIAI + HA+K  I   CSAGN+GP   TV
Sbjct: 263 AAFDEAISDGVDIISISIGGASLPFFEDPIAIGAFHAMKRGILTTCSAGNNGPGLFTV 320

[Close]
BLAST of CmaCh16G009840.1 vs. TAIR 10
Match: AT1G20150.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 68.2 bits (165), Expect = 5.2e-12
Identity = 35/60 (58.33%), Postives = 44/60 (73.33%), Query Frame = 0 

Query: 48  VTAAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTV 107
           + AA  DAI+DGVDV+SIS+G  P    +DP++I S HAV+  ITVVCS GNSGPS  +V
Sbjct: 266 ILAAFDDAIADGVDVISISMGLWPDNLLEDPLSIGSFHAVERGITVVCSVGNSGPSSQSV 325

[Close]
BLAST of CmaCh16G009840.1 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 67.4 bits (163), Expect = 8.8e-12
Identity = 37/60 (61.67%), Postives = 44/60 (73.33%), Query Frame = 0 

Query: 48  VTAAISDAISDGVDVLSISLGGSPLEFHDDPIAIASCHAVKNDITVVCSAGNSGPSPGTV 107
           V AAI  AI DGVDV+S+SLGGS   F+ DPIAIA   A++ +I V CSAGNSGP+  TV
Sbjct: 276 VIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGFGAMQKNIFVSCSAGNSGPTASTV 335

The following BLAST results are available for this feature:
BLAST of CmaCh16G009840.1 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F4JXC57.8e-1365.00Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... [more]
Q9ZSP58.6e-1260.00Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... [more]
Q9LZS62.5e-1158.62Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3... [more]
F4HSQ27.3e-1158.33Subtilisin-like protease SBT5.1 OS=Arabidopsis thaliana OX=3702 GN=SBT5.1 PE=3 S... [more]
Q9LNU11.2e-1055.56CO(2)-response secreted protease OS=Arabidopsis thaliana OX=3702 GN=CRSP PE=2 SV... [more]
BLAST of CmaCh16G009840.1 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G59810.15.5e-1465.00Subtilase family protein [more]
AT2G04160.16.1e-1360.00Subtilisin-like serine endopeptidase family protein [more]
AT5G03620.11.8e-1258.62Subtilisin-like serine endopeptidase family protein [more]
AT1G20150.15.2e-1258.33Subtilisin-like serine endopeptidase family protein [more]
AT1G01900.18.8e-1261.67subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 48..108
e-value: 2.6E-21
score: 77.9
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 45..110
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 48..109
e-value: 3.8E-7
score: 29.7
NoneNo IPR availablePANTHERPTHR43806PEPTIDASE S8coord: 49..108
NoneNo IPR availablePANTHERPTHR43806:SF34SUBTILISIN-LIKE PROTEASE SBT5.4coord: 49..108

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmaCh16G009840CmaCh16G009840gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh16G009840.1:exon:824CmaCh16G009840.1:exon:824exon
CmaCh16G009840.1:exon:825CmaCh16G009840.1:exon:825exon
CmaCh16G009840.1:exon:826CmaCh16G009840.1:exon:826exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh16G009840.1:cdsCmaCh16G009840.1:cds_3CDS
CmaCh16G009840.1:cdsCmaCh16G009840.1:cds_2CDS
CmaCh16G009840.1:cdsCmaCh16G009840.1:cdsCDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmaCh16G009840.1CmaCh16G009840.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity