CmaCh12G008750 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh12G008750
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Mechanosensitive ion channel protein 1
Location	Cma_Chr12: 6573278 .. 6577896 (-)
RNA-Seq Expression	CmaCh12G008750
Synteny	CmaCh12G008750

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATAAACAAAAAACGGTGAAAGATACTCGTAAAGAACGTTCGAAAATTCGGGGGTGGTTGGAAAAAGCAGAATCGAGAGACTAAGACGTGGGAAAAGTAAAAGGAGAGTTGCAATAGTGAAAGGAAAGTATAGATATAGAGAGGAAGTCCCGAAGGATGGAGACGGGGGAGAGGATCTGAATCAAGTTGAATAGGTTATTAGAACTAATTGTTGCTTCATTCAGATTTCTCTATAACAGGTTAGTGAATTTGAACTTTCTGCAGATGTATGTTCTAGTTGCAAGATTATACTGGAGAAGATTTCGTTTCAATTTAACAGTTACTTGTGTTTGATTGTTGTTTTACTGGCAGTGTAGCAAGGATAGGAGATGTGATTTGAGTCATTTGATTCGGTTTTTTTATGTTTTTTCTCATTTTTCTTCCATTTATTTGCTTCCAGATTATGGTTTTGGGTACATGGATGTAATTGTAGCCAGTAATTATGCTGCTTGTGTTTCTATTATGTGTCTCCGCTGTGAATTCTTTGTGATGTTGAATCAATTTGTTCTTGGATTTCCATAGCTGAATATTTTTTCTTGCAGTTTTGGAACTCAATTGATAAATCTTGAGTTCAGTTTGGTTCTTACGGAATTTACTAGACGTGTGCTGAATTGTATCACTGGTTGGCATGAGGCTTTTCATTCTCAATTCGATTTATAGTGCCTCCAAATCTTATTTGAATCCGTGCTCATTTCAATATTGCAAGTCTTACACAAAGATTTCTGAGTATGTTAACCGAGTTCTTAGACCGAGTGTTGTTCTAAATAAACAATACTACAAAGATGAATCTCACAGCTTGAAGAACGTCTCACGTACTGGTTTCATAAGAGCACCCTCTGTATGTCGTCTACACACACCAAGTTATGGTACACATCCCATTGCTACTTTTTCCCCGGCATCACCAGTGAAATATTGGAACACTATCCCTCCTACATCTCTTGCCTATCTGTCAAACCCTCGATTCTATTCATCGACTTCTAGCAGGAAAGCGGATATTACTGGAGATACTGGTGTTCCACTAGCTTCTTCTGGTGTTGAAGCAGATATCAGTAACCCCCCCGATGTTGGAAGAGACTGGCTTGAAAGAGTCAAGGATGGCTGGCAGAGTGCTGTCGATGCAGCATCTTTTACCGGACAGAAGGCTAGAGAAGTGTCTGATGAATTCTCTCCTCATGTAGATAAGATCTTAGATTCACATCCATATCTGAAGAATGAAATTGCTCCTGTTGGTATGACCTTAACTGCTACTCTTTTGGCTTGGGTGGTAATGCCTAGGCTTTTGAGGAGATTCCACAAGTATTCAATGCGAAGTCCTGTTGCCATACTTTCTGGAAGCACATCTATTGAGGAAATTCCCTATGAGAAAAGCTTTTGGGGTGCCTTAGAGGATCCTTTGAGATATCTTGTTACCTTCTATGCATTTTCTCAAATGTTAGTGACTCAAACTTCAACATATTTCTCTTTAATTACACAATGGTTTGTTGACTGTGTGCTTGATCTTTGCTTGAAACATATGTCTAAACTTTGATTTGCAGTGGCAAGATGGTTGCACCAACTGCCATAGCATCAGAGTTTGTATCACAGGCGTGCAGGGGGGCTGTCATCCTTTCTCTTGTCTGGTTTATTTATCGCTGGAAAACAAATGTGTTCTCTCGAGCATTGGCCTCCCAAAATTTTTCAGGGCTTGATCGGGAAAAGTTACTTACCCTCGACAAAGTTTCATCTGTTGGCCTATTTGCAATCGGATTAATGGCTTTGGCTGAGGCATCTGGGGTGGCTGTTCAATCTATGTTGACAGTTGGTGGCATTGGAGGTGAGAGCTTGCTGCCAAATGGTTTTGCAGTTTTACTTTCGTTTGACTGATTCCTAGTTTGCTGTCATGTGTCCTCCACTTGATTTGTTTATTCGTAGGGCCGAAGGTTTAACATTGTTCATCATTCTTTTTTCAAATAAAAATAAAAAAGAGAGAAAACTCTTCTCTTGATTATTAAACCTTACCGATGTAGCTCTTTTTGTATTGTTGTGGAGTGGAGTTATGCTCAAGATAACAATCATGTTCTTCGTAATGGTATGCTTAGATTTTTAGAGCGGTAATCAGGCTTGCATACTTTTAATAACTCGAAATATGTATAGCATGATTTAAATCTCTGACTTCTTTGCAGGAGTGGCCACTGCTTTTGCTTCGAGGGATATACTTGGGAATGTTTTAAGTGGCTTAACAATGCAGTTTTCACAACCCTTCTCACTTGGAGATACAATTAAGGTGTGTAAATCATACTTTTTCTCTTTTAGCTTTGGGCAAGGAACCAAGTTGTATCTAGCATTGTTGGGGCACATACAATCCACAAAAGTTGGAATATTTGAAGTGGGACTTCATTTTTTTTCCCCTTCTTTTACTACTATCATTCTTATTCTCCTAATAAAAACGTCACTGAAGTTGATACGTCAATGATGCTTGGTCTTTTAGATGACATTCATCTTTGTATACTATCAAATCCATGTGTACTTTTCAGTCATTGATCTCTCTTTGCATTTACTGGGAGATGATGGCATTGAACAAATTGCTGGAAACTTATAGATTGTTTCTGCAAGTTTGTTCTATTTGTTCTTTCTCATCAACTTCAAAAGCGAACGATTTGCAATTTTCCATTTGCAGGCTGGTGCTATTGAAGGCCAAGTGATTGAAATGGGCCTCATGACGACATCATTATTAAGTGCTGAAAAATTCCCAATCGTTGTTCCAAATTCTCTCTTTTCTAGCCAGGTGAATCTTCTTTTTTTCGTTTTTGCTTTTTTGTGCTTGGATTTTATAATTATTTTATCTTCATGGTCGTTTTATTTTTGTTCGTGAACTCAATGCTTCCCTTATTTAAATTGTTTACTAAAAGTCGAGGCATATTTGACCATTAGTTTCCATAGCCGAACGATGCATATGTGGCAAATAACTTGATTAAGTACATTTGTTGTCATGAAGATGTGTTGCCAATAATATGATTAAATACATTTGTTATCATGGAGATTTAAGTGATATTGGCTTTCTATTTGAAATTAGCGTAGAAGATAGAGTAAATTTGTTGTCCTCTCGATGCCTTTCCTCATGAAGAGCATGTGAATACGTCCAACTTCATTTTTCTGGTAGTCTCGCCAGTAGATTTCTAAATTAAGTTAATTATCCATTTTGATATCTTTATAAATTTTATTAAAAATATACCATTTTTAGCTCCCGTACAGAGAAAAATCGACCAATATTCAACTTTTCGGACTTGGAGATGGTCAATAGTCACGACACGTATGGCAATTGTTCTTATCTTTCACTCGGCAAACTTTCCTGTAATGCTAGATCATTTAATCTATGTTTGATTCTTTAATTCATGCAGGGATTTCTTTGGCATATGATTTGTTGTGTTTTAATGATTTCGCATGAATCTCTTAGCTGATTATGTAATGAAGGTATTTGTTCATTTGGTCCCACATTTTTCAACCCGTTTCTTGAATTTATGCTGAGGACTGCGATGGATTTCTTGGATTAAAGATGGTGATTTTGATCAATTTTACTGTGTGGTTAAATGGGATTATAATATTTACAGGTAATTATAAATAAGTCGCGGGCGCAATGGCGTGCAATCACAAAGAAAATTCCTTTCCAAGTTGCTGATTTTAGTACGGTTTCTAAGATATCAGATGATATTAAGAGTATGCTTAGGTCTCACCCTAAGGTATTCTTGGGCAAGGAGGCTCCTTATTGCTTCCTCTCTCAAATTGAAAGTTCGTATGCAGAGCTGACAATTGGTTGCAACCTTAAAAGGATGGTGAGTCAGTCTAATATTTTGAGTGTCATATGCTAGGAATCACAACTCTCTACGATGGTATGATATGCTCCATTTTGAGCAAGCTCTCGTGGCTTTGCTTTGGGCTTCCCTAAAATCCCTCGTACCAATGGAGATGTATTCCTTACTTATAAATCCATGATCATTCCCTTAATTAACTAACATGGGACTCCCTCCCAGCAATCCTCAACAATCCTCTCTTCGAACAAATTACACCATAGAGCCTCCCTTAAGGCTTATGGAGCCCTTGCACAGCCTCCCCTTAATTGAGGCTCAACTCCTTTCTCGAGAGTTCTCGAACAAAGTACACCTTTTGTTCGACACTTGAGTCACTTTGACTACACTCTCGAGGGTCCCAACTTCTTTGTTCGACATTTGAGGATTCTATTGACATGACTAAGTTAAGAGCATGACTTTGATACCATGTTAGGAATCGCGGATCTCCACAATGATATGATAATGTCCACTTTGAGCATAAGCTCTCATGACTTTGCATACCAATGGTGATGTATTCCTTACTTATAAACCCATGATCTTTCCCTTAATTAGCTAATGTGGGACTCCCTCCATGCAATCATCAACATCATATCCTCCTCGAACTCCTCGACTTGTTGTCCCTTCAAACGTTAGAGTTTCATACTTCAAAATTGTGTTTATGTAGGGTAAAGAAGAACTTTTCTCTACAGAGCAAGATATTCTCCTCCAGTCAGTACAGATCATCAAAGAAAATGGTGCAACCTTGGGTAACTGA

mRNA sequence

ATGATAAACAAAAAACGTGCCTCCAAATCTTATTTGAATCCGTGCTCATTTCAATATTGCAAGTCTTACACAAAGATTTCTGAGTATGTTAACCGAGTTCTTAGACCGAGTGTTGTTCTAAATAAACAATACTACAAAGATGAATCTCACAGCTTGAAGAACGTCTCACGTACTGGTTTCATAAGAGCACCCTCTGTATGTCGTCTACACACACCAAGTTATGGTACACATCCCATTGCTACTTTTTCCCCGGCATCACCAGTGAAATATTGGAACACTATCCCTCCTACATCTCTTGCCTATCTGTCAAACCCTCGATTCTATTCATCGACTTCTAGCAGGAAAGCGGATATTACTGGAGATACTGGTGTTCCACTAGCTTCTTCTGGTGTTGAAGCAGATATCAGTAACCCCCCCGATGTTGGAAGAGACTGGCTTGAAAGAGTCAAGGATGGCTGGCAGAGTGCTGTCGATGCAGCATCTTTTACCGGACAGAAGGCTAGAGAAGTGTCTGATGAATTCTCTCCTCATGTAGATAAGATCTTAGATTCACATCCATATCTGAAGAATGAAATTGCTCCTGTTGGTATGACCTTAACTGCTACTCTTTTGGCTTGGGTGGTAATGCCTAGGCTTTTGAGGAGATTCCACAAGTATTCAATGCGAAGTCCTGTTGCCATACTTTCTGGAAGCACATCTATTGAGGAAATTCCCTATGAGAAAAGCTTTTGGGGTGCCTTAGAGGATCCTTTGAGATATCTTGTTACCTTCTATGCATTTTCTCAAATTGGCAAGATGGTTGCACCAACTGCCATAGCATCAGAGTTTGTATCACAGGCGTGCAGGGGGGCTGTCATCCTTTCTCTTGTCTGGTTTATTTATCGCTGGAAAACAAATGTGTTCTCTCGAGCATTGGCCTCCCAAAATTTTTCAGGGCTTGATCGGGAAAAGTTACTTACCCTCGACAAAGTTTCATCTGTTGGCCTATTTGCAATCGGATTAATGGCTTTGGCTGAGGCATCTGGGGTGGCTGTTCAATCTATGTTGACAGTTGGTGGCATTGGAGGAGTGGCCACTGCTTTTGCTTCGAGGGATATACTTGGGAATGTTTTAAGTGGCTTAACAATGCAGTTTTCACAACCCTTCTCACTTGGAGATACAATTAAGGCTGGTGCTATTGAAGGCCAAGTGATTGAAATGGGCCTCATGACGACATCATTATTAAGTGCTGAAAAATTCCCAATCGTTGTTCCAAATTCTCTCTTTTCTAGCCAGGTAATTATAAATAAGTCGCGGGCGCAATGGCGTGCAATCACAAAGAAAATTCCTTTCCAAGTTGCTGATTTTAGTACGGTTTCTAAGATATCAGATGATATTAAGAGTATGCTTAGGTCTCACCCTAAGGTATTCTTGGGCAAGGAGGCTCCTTATTGCTTCCTCTCTCAAATTGAAAAGCAAGATATTCTCCTCCAGTCAGTACAGATCATCAAAGAAAATGGTGCAACCTTGGGTAACTGA

Coding sequence (CDS)

ATGATAAACAAAAAACGTGCCTCCAAATCTTATTTGAATCCGTGCTCATTTCAATATTGCAAGTCTTACACAAAGATTTCTGAGTATGTTAACCGAGTTCTTAGACCGAGTGTTGTTCTAAATAAACAATACTACAAAGATGAATCTCACAGCTTGAAGAACGTCTCACGTACTGGTTTCATAAGAGCACCCTCTGTATGTCGTCTACACACACCAAGTTATGGTACACATCCCATTGCTACTTTTTCCCCGGCATCACCAGTGAAATATTGGAACACTATCCCTCCTACATCTCTTGCCTATCTGTCAAACCCTCGATTCTATTCATCGACTTCTAGCAGGAAAGCGGATATTACTGGAGATACTGGTGTTCCACTAGCTTCTTCTGGTGTTGAAGCAGATATCAGTAACCCCCCCGATGTTGGAAGAGACTGGCTTGAAAGAGTCAAGGATGGCTGGCAGAGTGCTGTCGATGCAGCATCTTTTACCGGACAGAAGGCTAGAGAAGTGTCTGATGAATTCTCTCCTCATGTAGATAAGATCTTAGATTCACATCCATATCTGAAGAATGAAATTGCTCCTGTTGGTATGACCTTAACTGCTACTCTTTTGGCTTGGGTGGTAATGCCTAGGCTTTTGAGGAGATTCCACAAGTATTCAATGCGAAGTCCTGTTGCCATACTTTCTGGAAGCACATCTATTGAGGAAATTCCCTATGAGAAAAGCTTTTGGGGTGCCTTAGAGGATCCTTTGAGATATCTTGTTACCTTCTATGCATTTTCTCAAATTGGCAAGATGGTTGCACCAACTGCCATAGCATCAGAGTTTGTATCACAGGCGTGCAGGGGGGCTGTCATCCTTTCTCTTGTCTGGTTTATTTATCGCTGGAAAACAAATGTGTTCTCTCGAGCATTGGCCTCCCAAAATTTTTCAGGGCTTGATCGGGAAAAGTTACTTACCCTCGACAAAGTTTCATCTGTTGGCCTATTTGCAATCGGATTAATGGCTTTGGCTGAGGCATCTGGGGTGGCTGTTCAATCTATGTTGACAGTTGGTGGCATTGGAGGAGTGGCCACTGCTTTTGCTTCGAGGGATATACTTGGGAATGTTTTAAGTGGCTTAACAATGCAGTTTTCACAACCCTTCTCACTTGGAGATACAATTAAGGCTGGTGCTATTGAAGGCCAAGTGATTGAAATGGGCCTCATGACGACATCATTATTAAGTGCTGAAAAATTCCCAATCGTTGTTCCAAATTCTCTCTTTTCTAGCCAGGTAATTATAAATAAGTCGCGGGCGCAATGGCGTGCAATCACAAAGAAAATTCCTTTCCAAGTTGCTGATTTTAGTACGGTTTCTAAGATATCAGATGATATTAAGAGTATGCTTAGGTCTCACCCTAAGGTATTCTTGGGCAAGGAGGCTCCTTATTGCTTCCTCTCTCAAATTGAAAAGCAAGATATTCTCCTCCAGTCAGTACAGATCATCAAAGAAAATGGTGCAACCTTGGGTAACTGA

Protein sequence

MINKKRASKSYLNPCSFQYCKSYTKISEYVNRVLRPSVVLNKQYYKDESHSLKNVSRTGFIRAPSVCRLHTPSYGTHPIATFSPASPVKYWNTIPPTSLAYLSNPRFYSSTSSRKADITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSAVDAASFTGQKAREVSDEFSPHVDKILDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYSMRSPVAILSGSTSIEEIPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQACRGAVILSLVWFIYRWKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGVAVQSMLTVGGIGGVATAFASRDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVIEMGLMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPFQVADFSTVSKISDDIKSMLRSHPKVFLGKEAPYCFLSQIEKQDILLQSVQIIKENGATLGN

Homology

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BLAST of CmaCh12G008750 vs. ExPASy Swiss-Prot
Match: Q8VZL4 (Mechanosensitive ion channel protein 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MSL1 PE=1 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 1.2e-139
Identity = 260/442 (58.82%), Postives = 332/442 (75.11%), Query Frame = 0


Query: 96  PTSLAYLSNPRFYS----------STSSRKADITGDTGVPLASSGVEADISNPPDVGRDW 155
           P+ +++ SN R ++          S+ +R      D    + +SGV     +    G DW
Sbjct: 49  PSMISFSSNIRLHNDAKPFNYLGHSSYARAFSSKSDDFGSIVASGVTGS-GDGNGNGNDW 108

Query: 156 LERVKDGWQSAVDAASFTGQKAREVSDEFSPHVDKILDSHPYLKNEIAPVGMTLTATLLA 215
           +E+ KD  Q++VDA + T +K ++VSDE  PHV + LDS+PYLK+ I PV +T+T TL A
Sbjct: 109 VEKAKDVLQTSVDAVTETAKKTKDVSDEMIPHVQQFLDSNPYLKDVIVPVSLTMTGTLFA 168

Query: 216 WVVMPRLLRRFHKYSMRSPVAILSGSTSIEEIPYEKSFWGALEDPLRYLVTFYAFSQIGK 275
           WVVMPR+LRRFH Y+M+S   +L    S E++PYEKSFWGALEDP RYLVTF AF+QI  
Sbjct: 169 WVVMPRILRRFHTYAMQSSAKLLPVGFSNEDVPYEKSFWGALEDPARYLVTFIAFAQIAA 228

Query: 276 MVAPTAIASEFVSQACRGAVILSLVWFIYRWKTNVFSRALASQNFSGLDREKLLTLDKVS 335
           MVAPT IA+++ S   +GAVILSLVWF+YRWKTNV +R L++++F GLDREK+LTLDKVS
Sbjct: 229 MVAPTTIAAQYFSPTVKGAVILSLVWFLYRWKTNVITRMLSAKSFGGLDREKVLTLDKVS 288

Query: 336 SVGLFAIGLMALAEASGVAVQSMLTVGGIGGVATAFASRDILGNVLSGLTMQFSQPFSLG 395
           SVGLFAIGLMA AEA GVAVQS+LTVGG+GGVATAFA+RDILGNVLSGL+MQFS+PFS+G
Sbjct: 289 SVGLFAIGLMASAEACGVAVQSILTVGGVGGVATAFAARDILGNVLSGLSMQFSRPFSMG 348

Query: 396 DTIKAGAIEGQVIEMGLMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPFQ 455
           DTIKAG++EGQVIEMGL TTSLL+AEKFP++VPNSLFSSQVI+NKSRAQWRAI  KIP Q
Sbjct: 349 DTIKAGSVEGQVIEMGLTTTSLLNAEKFPVLVPNSLFSSQVIVNKSRAQWRAIASKIPLQ 408

Query: 456 VADFSTVSKISDDIKSMLRSHPKVFLGKEAPYCFLSQIEK-------------------- 505
           + D   + +IS++IK MLRS+ KVFLGKEAP+C+LS++EK                    
Sbjct: 409 IDDLDMIPQISNEIKEMLRSNTKVFLGKEAPHCYLSRVEKSFAELTIGCNLIRMGKEELY 468

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BLAST of CmaCh12G008750 vs. ExPASy Swiss-Prot
Match: P0AEB6 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=83334 GN=ynaI PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 2.5e-20
Identity = 74/242 (30.58%), Postives = 120/242 (49.59%), Query Frame = 0


Query: 284 AVILSLVWFIYRWKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGV 343
           AVIL++  F+     N F +    Q   G D      + ++  + +  + ++   E  G+
Sbjct: 87  AVILTIKLFLL---INQFEK---QQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGM 146

Query: 344 AVQSMLTVGGIGGVATAFASRDILGNVLSGLTMQFSQPFSLGDTIKA--GAIEGQVIEMG 403
           ++  +LT GGIGG+A   A +DIL N  SG+ + F +PFS+GD I++    IEG V E+G
Sbjct: 147 SLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIG 206

Query: 404 LMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPFQVADFSTVSKISDDIKS 463
              T + + +  P+ VPNSLFSS  + N  R   R IT  I  +  D + V  I + ++ 
Sbjct: 207 WRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVRE 266

Query: 464 MLRSHPK--------VFLGKEAP-------YCFLSQ-------IEKQDILLQSVQIIKEN 502
           ML++HP         V+  + A        YCF            +QD+ L+ + I++ +
Sbjct: 267 MLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSH 322

[Close]

BLAST of CmaCh12G008750 vs. ExPASy Swiss-Prot
Match: P0AEB5 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) OX=83333 GN=ynaI PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 2.5e-20
Identity = 74/242 (30.58%), Postives = 120/242 (49.59%), Query Frame = 0


Query: 284 AVILSLVWFIYRWKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEASGV 343
           AVIL++  F+     N F +    Q   G D      + ++  + +  + ++   E  G+
Sbjct: 87  AVILTIKLFLL---INQFEK---QQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGM 146

Query: 344 AVQSMLTVGGIGGVATAFASRDILGNVLSGLTMQFSQPFSLGDTIKA--GAIEGQVIEMG 403
           ++  +LT GGIGG+A   A +DIL N  SG+ + F +PFS+GD I++    IEG V E+G
Sbjct: 147 SLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIG 206

Query: 404 LMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPFQVADFSTVSKISDDIKS 463
              T + + +  P+ VPNSLFSS  + N  R   R IT  I  +  D + V  I + ++ 
Sbjct: 207 WRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVRE 266

Query: 464 MLRSHPK--------VFLGKEAP-------YCFLSQ-------IEKQDILLQSVQIIKEN 502
           ML++HP         V+  + A        YCF            +QD+ L+ + I++ +
Sbjct: 267 MLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSH 322

[Close]

BLAST of CmaCh12G008750 vs. ExPASy Swiss-Prot
Match: O25170 (Uncharacterized MscS family protein HP_0415 OS=Helicobacter pylori (strain ATCC 700392 / 26695) OX=85962 GN=HP_0415 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 6.1e-14
Identity = 58/202 (28.71%), Postives = 103/202 (50.99%), Query Frame = 0


Query: 283 GAV-ILSLVWFI---YRWKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALA 342
           GAV I+ L W +   ++         +A+++     +E +  + KV    +F + L+ + 
Sbjct: 323 GAVYIMLLAWLVIALFKGYGEALVTNMATKSTHNFRKEVINLILKVVYFLIFIVALLGVL 382

Query: 343 EASGVAVQSMLTVGGIGGVATAFASRDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVI 402
           +  G  V +++   GIGG+A A A +D+L N  + + +     FS GD I  G +EG V+
Sbjct: 383 KQLGFNVSAIIASLGIGGLAVALAVKDVLANFFASVILLLDNSFSQGDWIVCGEVEGTVV 442

Query: 403 EMGLMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPFQV-----ADFSTVS 462
           EMGL  T++ + +   + VPNS  + + I N SR   R + ++I  ++     +  S + 
Sbjct: 443 EMGLRRTTIRAFDNALLSVPNSELAGKPIRNWSR---RKVGRRIKMEIGLTYSSSQSALQ 502

Query: 463 KISDDIKSMLRSHPKVFLGKEA 476
               DIK ML +HPK+  G ++
Sbjct: 503 LCVKDIKEMLENHPKIANGADS 521

[Close]

BLAST of CmaCh12G008750 vs. ExPASy Swiss-Prot
Match: Q9ZKG7 (Uncharacterized MscS family protein jhp_0969 OS=Helicobacter pylori (strain J99 / ATCC 700824) OX=85963 GN=jhp_0969 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 7.9e-14
Identity = 58/202 (28.71%), Postives = 103/202 (50.99%), Query Frame = 0


Query: 283 GAV-ILSLVWFI---YRWKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALA 342
           GAV I+ L W +   ++         +A+++     +E +  + KV    +F + L+ + 
Sbjct: 323 GAVYIMLLAWLVIALFKGYGEALVTNVATKSTHNFRKEVINLILKVVYFLIFIVALLGVL 382

Query: 343 EASGVAVQSMLTVGGIGGVATAFASRDILGNVLSGLTMQFSQPFSLGDTIKAGAIEGQVI 402
           +  G  V +++   GIGG+A A A +D+L N  + + +     FS GD I  G +EG V+
Sbjct: 383 KQLGFNVSAIIASLGIGGLAVALAVKDVLANFFASVILLLDNSFSQGDWIVCGEVEGTVV 442

Query: 403 EMGLMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPFQV-----ADFSTVS 462
           EMGL  T++ + +   + VPNS  + + I N SR   R + ++I  ++     +  S + 
Sbjct: 443 EMGLRRTTIRAFDNALLSVPNSELAGKPIRNWSR---RKVGRRIKMEIGLTYSSSQSALQ 502

Query: 463 KISDDIKSMLRSHPKVFLGKEA 476
               DIK ML +HPK+  G ++
Sbjct: 503 LCVKDIKEMLENHPKIANGADS 521

[Close]

BLAST of CmaCh12G008750 vs. TAIR 10
Match: AT4G00290.1 (Mechanosensitive ion channel protein )

HSP 1 Score: 498.0 bits (1281), Expect = 8.6e-141
Identity = 260/442 (58.82%), Postives = 332/442 (75.11%), Query Frame = 0


Query: 96  PTSLAYLSNPRFYS----------STSSRKADITGDTGVPLASSGVEADISNPPDVGRDW 155
           P+ +++ SN R ++          S+ +R      D    + +SGV     +    G DW
Sbjct: 49  PSMISFSSNIRLHNDAKPFNYLGHSSYARAFSSKSDDFGSIVASGVTGS-GDGNGNGNDW 108

Query: 156 LERVKDGWQSAVDAASFTGQKAREVSDEFSPHVDKILDSHPYLKNEIAPVGMTLTATLLA 215
           +E+ KD  Q++VDA + T +K ++VSDE  PHV + LDS+PYLK+ I PV +T+T TL A
Sbjct: 109 VEKAKDVLQTSVDAVTETAKKTKDVSDEMIPHVQQFLDSNPYLKDVIVPVSLTMTGTLFA 168

Query: 216 WVVMPRLLRRFHKYSMRSPVAILSGSTSIEEIPYEKSFWGALEDPLRYLVTFYAFSQIGK 275
           WVVMPR+LRRFH Y+M+S   +L    S E++PYEKSFWGALEDP RYLVTF AF+QI  
Sbjct: 169 WVVMPRILRRFHTYAMQSSAKLLPVGFSNEDVPYEKSFWGALEDPARYLVTFIAFAQIAA 228

Query: 276 MVAPTAIASEFVSQACRGAVILSLVWFIYRWKTNVFSRALASQNFSGLDREKLLTLDKVS 335
           MVAPT IA+++ S   +GAVILSLVWF+YRWKTNV +R L++++F GLDREK+LTLDKVS
Sbjct: 229 MVAPTTIAAQYFSPTVKGAVILSLVWFLYRWKTNVITRMLSAKSFGGLDREKVLTLDKVS 288

Query: 336 SVGLFAIGLMALAEASGVAVQSMLTVGGIGGVATAFASRDILGNVLSGLTMQFSQPFSLG 395
           SVGLFAIGLMA AEA GVAVQS+LTVGG+GGVATAFA+RDILGNVLSGL+MQFS+PFS+G
Sbjct: 289 SVGLFAIGLMASAEACGVAVQSILTVGGVGGVATAFAARDILGNVLSGLSMQFSRPFSMG 348

Query: 396 DTIKAGAIEGQVIEMGLMTTSLLSAEKFPIVVPNSLFSSQVIINKSRAQWRAITKKIPFQ 455
           DTIKAG++EGQVIEMGL TTSLL+AEKFP++VPNSLFSSQVI+NKSRAQWRAI  KIP Q
Sbjct: 349 DTIKAGSVEGQVIEMGLTTTSLLNAEKFPVLVPNSLFSSQVIVNKSRAQWRAIASKIPLQ 408

Query: 456 VADFSTVSKISDDIKSMLRSHPKVFLGKEAPYCFLSQIEK-------------------- 505
           + D   + +IS++IK MLRS+ KVFLGKEAP+C+LS++EK                    
Sbjct: 409 IDDLDMIPQISNEIKEMLRSNTKVFLGKEAPHCYLSRVEKSFAELTIGCNLIRMGKEELY 468

[Close]

BLAST of CmaCh12G008750 vs. TAIR 10
Match: AT4G00234.1 (BEST Arabidopsis thaliana protein match is: Mechanosensitive ion channel protein (TAIR:AT4G00290.1); Has 57 Blast hits to 57 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 56; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 280.0 bits (715), Expect = 3.7e-75
Identity = 145/252 (57.54%), Postives = 187/252 (74.21%), Query Frame = 0


Query: 101 YLSNPRFYSSTSSRKADITGDTGVPLASSGVEADISNPPDVGRDWLERVKDGWQSAVDAA 160
           YLS+  +  + SS+  D        + +SGV     +    G DW+E+ KD  Q++V+A 
Sbjct: 19  YLSHSSYARAFSSKSDDFGS-----IVASGV---TGSGDGNGNDWVEKAKDVVQTSVNAV 78

Query: 161 SFTGQKAREVSDEFSPHVDKILDSHPYLKNEIAPVGMTLTATLLAWVVMPRLLRRFHKYS 220
           + T +K ++VSDE  PHV + LDS+PYLK+ I PV +T+T TL AW+VMPR+LRRFH Y+
Sbjct: 79  AETAKKTKDVSDEMIPHVQQFLDSNPYLKDVIVPVSLTVTGTLFAWIVMPRILRRFHTYA 138

Query: 221 MRSPVAILSGSTSIEEIPYEKSFWGALEDPLRYLVTFYAFSQIGKMVAPTAIASEFVSQA 280
           M+S   +L    S E+IPYEKSFWGALEDP RYLVTF AF+QI  MVAPT IA+++ S  
Sbjct: 139 MQSSAKLLPVGFSNEDIPYEKSFWGALEDPARYLVTFIAFAQIAAMVAPTTIAAQYFSPT 198

Query: 281 CRGAVILSLVWFIYRWKTNVFSRALASQNFSGLDREKLLTLDKVSSVGLFAIGLMALAEA 340
            +GAVILS+VWF+YRWKTNV +R L++++F GLDR+K+LTLDKVSSVGLFAIGLMA AEA
Sbjct: 199 VKGAVILSVVWFLYRWKTNVITRMLSAKSFGGLDRDKVLTLDKVSSVGLFAIGLMASAEA 258

Query: 341 SGVAVQSMLTVG 353
            GVAVQS+LTVG
Sbjct: 259 CGVAVQSILTVG 262

[Close]

BLAST of CmaCh12G008750 vs. TAIR 10
Match: AT1G58200.1 (MSCS-like 3 )

HSP 1 Score: 58.9 bits (141), Expect = 1.3e-08
Identity = 45/159 (28.30%), Postives = 79/159 (49.69%), Query Frame = 0


Query: 339 EASGVAVQSMLTVGGIGGVATAFASRDILGNVLSGLTMQFSQPFSLGDTI--KAGAIE-- 398
           E  G + Q  LT GG+G V    A R+IL N LS + +  ++PF L + I  K G  E  
Sbjct: 257 ELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIMIHATRPFVLNEWIQTKIGGYEVS 316

Query: 399 GQVIEMGLMTTSLLSA-EKFPIVVPNSLFSSQVIIN-KSRAQWRAITKKIPFQVADFSTV 458
           G V ++G  + +++   ++  + +PN  FS  ++ N   +  WR I   +     D S +
Sbjct: 317 GTVEQVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTHWR-IKTHLAISHLDVSKI 376

Query: 459 SKISDDIKSMLRSHPKVFLGKEAPYCFLSQIEKQDILLQ 492
           + I  D++ +L  +P++   K     FL  I+ ++  L+
Sbjct: 377 NNIVADMRKVLSKNPQIEQQKIHRRVFLEDIDPENQALR 414

[Close]

BLAST of CmaCh12G008750 vs. TAIR 10
Match: AT1G58200.2 (MSCS-like 3 )

HSP 1 Score: 58.9 bits (141), Expect = 1.3e-08
Identity = 45/159 (28.30%), Postives = 79/159 (49.69%), Query Frame = 0


Query: 339 EASGVAVQSMLTVGGIGGVATAFASRDILGNVLSGLTMQFSQPFSLGDTI--KAGAIE-- 398
           E  G + Q  LT GG+G V    A R+IL N LS + +  ++PF L + I  K G  E  
Sbjct: 257 ELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIMIHATRPFVLNEWIQTKIGGYEVS 316

Query: 399 GQVIEMGLMTTSLLSA-EKFPIVVPNSLFSSQVIIN-KSRAQWRAITKKIPFQVADFSTV 458
           G V ++G  + +++   ++  + +PN  FS  ++ N   +  WR I   +     D S +
Sbjct: 317 GTVEQVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTHWR-IKTHLAISHLDVSKI 376

Query: 459 SKISDDIKSMLRSHPKVFLGKEAPYCFLSQIEKQDILLQ 492
           + I  D++ +L  +P++   K     FL  I+ ++  L+
Sbjct: 377 NNIVADMRKVLSKNPQIEQQKIHRRVFLEDIDPENQALR 414

[Close]

BLAST of CmaCh12G008750 vs. TAIR 10
Match: AT5G10490.1 (MSCS-like 2 )

HSP 1 Score: 52.8 bits (125), Expect = 9.6e-07
Identity = 43/151 (28.48%), Postives = 74/151 (49.01%), Query Frame = 0


Query: 325 SSVGLFAIGLMALAEASGVAVQSMLTVGGIGGVATAFASRDILGNVLSGLTMQFSQPFSL 384
           S+V + A+ L    E  G + Q  LT GG+G V    A R+IL N LS + +  ++PF L
Sbjct: 243 SAVWVAAVSL--FMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVL 302

Query: 385 GD----TIKAGAIEGQVIEMGLMTTSLLSAE-KFPIVVPNSLFSSQVIIN-KSRAQWRAI 444
            +     I+   + G V  +G  + +++  E +  I +PN  F+  V+ N   +  WR I
Sbjct: 303 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWR-I 362

Query: 445 TKKIPFQVADFSTVSKISDDIKSMLRSHPKV 470
              +     D + ++ I  D++ +L  +P V
Sbjct: 363 KTHLAISHLDVNKINNIVADMRKVLAKNPMV 390

The following BLAST results are available for this feature:

BLAST of CmaCh12G008750 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VZL4	1.2e-139	58.82	Mechanosensitive ion channel protein 1, mitochondrial OS=Arabidopsis thaliana OX...	[more]
P0AEB6	2.5e-20	30.58	Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=833...	[more]
P0AEB5	2.5e-20	30.58	Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) O...	[more]
O25170	6.1e-14	28.71	Uncharacterized MscS family protein HP_0415 OS=Helicobacter pylori (strain ATCC ...	[more]
Q9ZKG7	7.9e-14	28.71	Uncharacterized MscS family protein jhp_0969 OS=Helicobacter pylori (strain J99 ...	[more]

BLAST of CmaCh12G008750 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G00290.1	8.6e-141	58.82	Mechanosensitive ion channel protein	[more]
AT4G00234.1	3.7e-75	57.54	BEST Arabidopsis thaliana protein match is: Mechanosensitive ion channel protein...	[more]
AT1G58200.1	1.3e-08	28.30	MSCS-like 3	[more]
AT1G58200.2	1.3e-08	28.30	MSCS-like 3	[more]
AT5G10490.1	9.6e-07	28.48	MSCS-like 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023408	Mechanosensitive ion channel MscS domain superfamily	GENE3D	2.30.30.60		coord: 381..429 e-value: 2.0E-12 score: 48.9
IPR006685	Mechanosensitive ion channel MscS	PFAM	PF00924	MS_channel	coord: 323..479 e-value: 5.4E-31 score: 107.7
None	No IPR available	GENE3D	1.10.287.1260		coord: 277..379 e-value: 3.0E-14 score: 55.1
None	No IPR available	PANTHER	PTHR30566	YNAI-RELATED MECHANOSENSITIVE ION CHANNEL	coord: 89..504
None	No IPR available	PANTHER	PTHR30566:SF5	MECHANOSENSITIVE ION CHANNEL PROTEIN 1, MITOCHONDRIAL-RELATED	coord: 89..504
IPR011014	Mechanosensitive ion channel MscS, transmembrane-2	SUPERFAMILY	82861	Mechanosensitive channel protein MscS (YggB), transmembrane region	coord: 282..363
IPR010920	LSM domain superfamily	SUPERFAMILY	50182	Sm-like ribonucleoproteins	coord: 365..430

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh12G008750.1	CmaCh12G008750.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006811	ion transport
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane