Bhi04G001721 (gene) Wax gourd (B227) v1

Overview
NameBhi04G001721
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionbasic blue protein-like
Locationchr4: 58471259 .. 58471471 (-)
RNA-Seq ExpressionBhi04G001721
SyntenyBhi04G001721
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTAGAATTCAACTACGACAAAAGAATTCACAACGTGGTGGCAGTGGATGAAATAAGTTATAGAAACTGCACGGCTCCAAGAGGTTCCAAAGTGTTCAATTCTGGCAACGATGAGATCAAGCTCTCAATAGGATACAATTTCTTCATATGCACCTTACCAGCACACTGCCAAGCTGGGATGAAGATTGTTGTTGCTGCCCGCGCCCCCTAA

mRNA sequence

ATGGTAGAATTCAACTACGACAAAAGAATTCACAACGTGGTGGCAGTGGATGAAATAAGTTATAGAAACTGCACGGCTCCAAGAGGTTCCAAAGTGTTCAATTCTGGCAACGATGAGATCAAGCTCTCAATAGGATACAATTTCTTCATATGCACCTTACCAGCACACTGCCAAGCTGGGATGAAGATTGTTGTTGCTGCCCGCGCCCCCTAA

Coding sequence (CDS)

ATGGTAGAATTCAACTACGACAAAAGAATTCACAACGTGGTGGCAGTGGATGAAATAAGTTATAGAAACTGCACGGCTCCAAGAGGTTCCAAAGTGTTCAATTCTGGCAACGATGAGATCAAGCTCTCAATAGGATACAATTTCTTCATATGCACCTTACCAGCACACTGCCAAGCTGGGATGAAGATTGTTGTTGCTGCCCGCGCCCCCTAA

Protein sequence

MVEFNYDKRIHNVVAVDEISYRNCTAPRGSKVFNSGNDEIKLSIGYNFFICTLPAHCQAGMKIVVAARAP
Homology
BLAST of Bhi04G001721 vs. TAIR 10
Match: AT2G02850.1 (plantacyanin )

HSP 1 Score: 84.0 bits (206), Expect = 5.4e-17
Identity = 37/64 (57.81%), Postives = 43/64 (67.19%), Query Frame = 0

Query: 4   FNYDKRIHNVVAVDEISYRNCTAPRGSKVFNSGNDEIKLSIGYNFFICTLPAHCQAGMKI 63
           FNY+ R+HNVV VD  SY NC  P G+K + SG D I LS G NFFIC  P HC++ MKI
Sbjct: 65  FNYNPRMHNVVKVDSGSYNNCKTPTGAKPYTSGKDRITLSKGQNFFICNFPNHCESDMKI 124

Query: 64  VVAA 68
            V A
Sbjct: 125 AVTA 128

BLAST of Bhi04G001721 vs. TAIR 10
Match: AT1G17800.1 (early nodulin-like protein 22 )

HSP 1 Score: 62.0 bits (149), Expect = 2.2e-10
Identity = 28/71 (39.44%), Postives = 45/71 (63.38%), Query Frame = 0

Query: 1   MVEFNYDKRIHNVVAVDEISYRNCTAPRGSKVFNSGNDEIKLSIGYNFFICT-LPAHCQA 60
           ++EF YD +  N++ V+   Y  C A  G+  ++SG+D+I+L+ GYN+FI T  P  C  
Sbjct: 69  ILEFKYDYQRFNLIVVNRTGYETCEANVGAIEYSSGDDKIQLNYGYNYFIGTYTPEDCTT 128

Query: 61  GMKIVVAARAP 71
           G+K+ + A AP
Sbjct: 129 GLKMAIKALAP 139

BLAST of Bhi04G001721 vs. TAIR 10
Match: AT5G26330.1 (Cupredoxin superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 1.4e-09
Identity = 26/63 (41.27%), Postives = 37/63 (58.73%), Query Frame = 0

Query: 2   VEFNYDKRIHNVVAVDEISYRNCTAPRGSKVFNSGNDEIKL-SIGYNFFICTLPAHCQAG 61
           V F Y+ + HNV+ V    YR+C   +    F +GND I L + G++FF C +P HC AG
Sbjct: 55  VLFEYNPQFHNVMRVTHPMYRSCNTSKPISTFTTGNDSITLTNHGHHFFFCGVPGHCLAG 114

Query: 62  MKI 64
            K+
Sbjct: 115 QKL 117

BLAST of Bhi04G001721 vs. TAIR 10
Match: AT2G32300.1 (uclacyanin 1 )

HSP 1 Score: 49.3 bits (116), Expect = 1.5e-06
Identity = 23/63 (36.51%), Postives = 35/63 (55.56%), Query Frame = 0

Query: 4   FNYDKRIHNVVAVDEISYRNCTAPRGSKVFNSGNDEIKLSI-GYNFFICTLPAHCQAGMK 63
           F+Y    H+VV V +  + +C A +    F +GN  + L+  G  +FIC +P HC  GMK
Sbjct: 57  FSYPAAFHDVVEVTKPEFDSCQAVKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMK 116

Query: 64  IVV 66
           + V
Sbjct: 117 LEV 119

BLAST of Bhi04G001721 vs. TAIR 10
Match: AT3G60270.1 (Cupredoxin superfamily protein )

HSP 1 Score: 48.5 bits (114), Expect = 2.5e-06
Identity = 24/69 (34.78%), Postives = 37/69 (53.62%), Query Frame = 0

Query: 2   VEFNYDKRIHNVVAVDEISYRNCTAPRGSKVFNSGNDEIKLS-IGYNFFICTLPAHCQAG 61
           +EF Y    H+V  V++  Y  C   R ++ F+ G+ +I L+ +G   F+C  P HC  G
Sbjct: 55  LEFKYGPS-HSVAVVNKADYDGCETSRPTQSFSDGDTKIDLTKVGAIHFLCLTPGHCSLG 114

Query: 62  MKIVVAARA 70
           MK+ V   A
Sbjct: 115 MKLAVQVLA 122

BLAST of Bhi04G001721 vs. ExPASy Swiss-Prot
Match: Q8LG89 (Basic blue protein OS=Arabidopsis thaliana OX=3702 GN=ARPN PE=2 SV=2)

HSP 1 Score: 84.0 bits (206), Expect = 7.6e-16
Identity = 37/64 (57.81%), Postives = 43/64 (67.19%), Query Frame = 0

Query: 4   FNYDKRIHNVVAVDEISYRNCTAPRGSKVFNSGNDEIKLSIGYNFFICTLPAHCQAGMKI 63
           FNY+ R+HNVV VD  SY NC  P G+K + SG D I LS G NFFIC  P HC++ MKI
Sbjct: 65  FNYNPRMHNVVKVDSGSYNNCKTPTGAKPYTSGKDRITLSKGQNFFICNFPNHCESDMKI 124

Query: 64  VVAA 68
            V A
Sbjct: 125 AVTA 128

BLAST of Bhi04G001721 vs. ExPASy Swiss-Prot
Match: P60496 (Chemocyanin OS=Lilium longiflorum OX=4690 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 3.8e-15
Identity = 35/64 (54.69%), Postives = 46/64 (71.88%), Query Frame = 0

Query: 4   FNYDKRIHNVVAVDEISYRNCTAPRGSKVFNSGNDEIKLSIGYNFFICTLPAHCQAGMKI 63
           F Y+  +HNVV+V    Y++CTA  GS+VF SG+D I LS G N+FIC++P HCQ G+KI
Sbjct: 62  FKYNPAVHNVVSVPAGGYKSCTASPGSRVFKSGDDRITLSRGTNYFICSVPGHCQGGLKI 121

Query: 64  VVAA 68
            V A
Sbjct: 122 AVTA 125

BLAST of Bhi04G001721 vs. ExPASy Swiss-Prot
Match: P00303 (Basic blue protein OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 1.4e-14
Identity = 33/64 (51.56%), Postives = 44/64 (68.75%), Query Frame = 0

Query: 4  FNYDKRIHNVVAVDEISYRNCTAPRGSKVFNSGNDEIKLSIGYNFFICTLPAHCQAGMKI 63
          FNY+  +HNVV V++  +  C  P G+KV+ SG D+IKL  G ++FIC  P HCQ+GMKI
Sbjct: 32 FNYNPXMHNVVVVNQGGFSTCNTPAGAKVYTSGRDQIKLPKGQSYFICNFPGHCQSGMKI 91

Query: 64 VVAA 68
           V A
Sbjct: 92 AVNA 95

BLAST of Bhi04G001721 vs. ExPASy Swiss-Prot
Match: P00302 (Stellacyanin OS=Toxicodendron vernicifluum OX=4013 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 5.8e-08
Identity = 22/61 (36.07%), Postives = 37/61 (60.66%), Query Frame = 0

Query: 4  FNYDKRIHNVVAVDEISYRNCTAPRGSKVFNSGNDEIKL-SIGYNFFICTLPAHCQAGMK 63
          F YD+R HNV  V + +Y++C        +N+G++ I L ++G  ++IC +P HC  G K
Sbjct: 39 FKYDRRFHNVDKVTQKNYQSCNDTTPIASYNTGBBRINLKTVGQKYYICGVPKHCDLGQK 98

BLAST of Bhi04G001721 vs. ExPASy Swiss-Prot
Match: P80728 (Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 9.9e-08
Identity = 23/63 (36.51%), Postives = 38/63 (60.32%), Query Frame = 0

Query: 4  FNYDKRIHNVVAVDEISYRNCTAPRGSKVFNSGNDEIKLS-IGYNFFICTLPAHCQAGMK 63
          FNY+ + HNV+ VD+  +++C +   +  + SG D I L   G  +F+C +P HCQ G K
Sbjct: 37 FNYNNKFHNVLQVDQEQFKSCNSSSPAASYTSGADSIPLKRPGTFYFLCGIPGHCQLGQK 96

Query: 64 IVV 66
          + +
Sbjct: 97 VEI 99

BLAST of Bhi04G001721 vs. ExPASy TrEMBL
Match: A0A6J1J2F7 (basic blue protein-like OS=Cucurbita maxima OX=3661 GN=LOC111480707 PE=4 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.2e-19
Identity = 46/65 (70.77%), Postives = 53/65 (81.54%), Query Frame = 0

Query: 1   MVEFNYDKRIHNVVAVDEISYRNCTAPRGSKVFNSGNDEIKLSIGYNFFICTLPAHCQAG 60
           ++ FNY+  +HNVVAVDE  YRNC APRG+KVFNSG DEI LS G NFFICT+P HCQAG
Sbjct: 63  ILRFNYNPTVHNVVAVDEGGYRNCIAPRGAKVFNSGKDEIMLSRGRNFFICTIPGHCQAG 122

Query: 61  MKIVV 66
           MKI +
Sbjct: 123 MKIAI 127

BLAST of Bhi04G001721 vs. ExPASy TrEMBL
Match: A0A6J1DI73 (basic blue protein-like OS=Momordica charantia OX=3673 GN=LOC111021165 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.5e-19
Identity = 46/67 (68.66%), Postives = 53/67 (79.10%), Query Frame = 0

Query: 1   MVEFNYDKRIHNVVAVDEISYRNCTAPRGSKVFNSGNDEIKLSIGYNFFICTLPAHCQAG 60
           ++ F YD R+HNVVAVDE  Y +CTAP G+KV +SGNDEIKLS G+NFFICT P HCQAG
Sbjct: 60  VLRFKYDPRVHNVVAVDEEGYNSCTAPTGTKVLSSGNDEIKLSPGHNFFICTTPGHCQAG 119

Query: 61  MKIVVAA 68
           MKI   A
Sbjct: 120 MKIATTA 126

BLAST of Bhi04G001721 vs. ExPASy TrEMBL
Match: A0A6J1FMC7 (basic blue protein-like OS=Cucurbita moschata OX=3662 GN=LOC111446527 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 7.6e-19
Identity = 45/65 (69.23%), Postives = 52/65 (80.00%), Query Frame = 0

Query: 1   MVEFNYDKRIHNVVAVDEISYRNCTAPRGSKVFNSGNDEIKLSIGYNFFICTLPAHCQAG 60
           ++ FNY+  +HNVVAVDE  YR+C APRG+KVFNSG DEI LS G NFFICT P HCQAG
Sbjct: 63  ILRFNYNPTVHNVVAVDEGGYRSCIAPRGAKVFNSGKDEIMLSRGQNFFICTTPGHCQAG 122

Query: 61  MKIVV 66
           MKI +
Sbjct: 123 MKIAI 127

BLAST of Bhi04G001721 vs. ExPASy TrEMBL
Match: A0A6J1FSU5 (basic blue protein-like OS=Cucurbita moschata OX=3662 GN=LOC111446556 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 7.6e-19
Identity = 45/65 (69.23%), Postives = 52/65 (80.00%), Query Frame = 0

Query: 1   MVEFNYDKRIHNVVAVDEISYRNCTAPRGSKVFNSGNDEIKLSIGYNFFICTLPAHCQAG 60
           ++ FNY+  +HNVVAVDE  YR+C APRG+KVFNSG DEI LS G NFFICT P HCQAG
Sbjct: 65  ILRFNYNPTVHNVVAVDEGGYRSCIAPRGAKVFNSGKDEIMLSRGQNFFICTTPGHCQAG 124

Query: 61  MKIVV 66
           MKI +
Sbjct: 125 MKIAI 129

BLAST of Bhi04G001721 vs. ExPASy TrEMBL
Match: A0A6J1FK99 (basic blue protein-like OS=Cucurbita moschata OX=3662 GN=LOC111446518 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 7.6e-19
Identity = 45/65 (69.23%), Postives = 52/65 (80.00%), Query Frame = 0

Query: 1   MVEFNYDKRIHNVVAVDEISYRNCTAPRGSKVFNSGNDEIKLSIGYNFFICTLPAHCQAG 60
           ++ FNY+  +HNVVAVDE  YR+C APRG+KVFNSG DEI LS G NFFICT P HCQAG
Sbjct: 63  ILRFNYNPTVHNVVAVDEGGYRSCIAPRGAKVFNSGKDEIMLSRGQNFFICTTPGHCQAG 122

Query: 61  MKIVV 66
           MKI +
Sbjct: 123 MKIAI 127

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G02850.15.4e-1757.81plantacyanin [more]
AT1G17800.12.2e-1039.44early nodulin-like protein 22 [more]
AT5G26330.11.4e-0941.27Cupredoxin superfamily protein [more]
AT2G32300.11.5e-0636.51uclacyanin 1 [more]
AT3G60270.12.5e-0634.78Cupredoxin superfamily protein [more]
Match NameE-valueIdentityDescription
Q8LG897.6e-1657.81Basic blue protein OS=Arabidopsis thaliana OX=3702 GN=ARPN PE=2 SV=2[more]
P604963.8e-1554.69Chemocyanin OS=Lilium longiflorum OX=4690 PE=1 SV=1[more]
P003031.4e-1451.56Basic blue protein OS=Cucumis sativus OX=3659 PE=1 SV=1[more]
P003025.8e-0836.07Stellacyanin OS=Toxicodendron vernicifluum OX=4013 PE=1 SV=1[more]
P807289.9e-0836.51Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1J2F71.2e-1970.77basic blue protein-like OS=Cucurbita maxima OX=3661 GN=LOC111480707 PE=4 SV=1[more]
A0A6J1DI731.5e-1968.66basic blue protein-like OS=Momordica charantia OX=3673 GN=LOC111021165 PE=4 SV=1[more]
A0A6J1FMC77.6e-1969.23basic blue protein-like OS=Cucurbita moschata OX=3662 GN=LOC111446527 PE=4 SV=1[more]
A0A6J1FSU57.6e-1969.23basic blue protein-like OS=Cucurbita moschata OX=3662 GN=LOC111446556 PE=4 SV=1[more]
A0A6J1FK997.6e-1969.23basic blue protein-like OS=Cucurbita moschata OX=3662 GN=LOC111446518 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003245Phytocyanin domainPFAMPF02298Cu_bind_likecoord: 2..61
e-value: 6.0E-16
score: 58.2
IPR003245Phytocyanin domainPROSITEPS51485PHYTOCYANINcoord: 1..69
score: 21.102554
IPR008972CupredoxinGENE3D2.60.40.420coord: 1..67
e-value: 8.5E-19
score: 69.3
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 2..66
NoneNo IPR availablePANTHERPTHR33021:SF379BLUE PROTEIN, PUTATIVE-RELATEDcoord: 2..66
IPR039391PhytocyaninPANTHERPTHR33021BLUE COPPER PROTEINcoord: 2..66

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi04M001721Bhi04M001721mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0022900 electron transport chain
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0009055 electron transfer activity