Lsi05G001810 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi05G001810
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionrRNA N-glycosidase
Locationchr05 : 2615176 .. 2615808 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAGTCCTGAAGACATACATCTTAGTAAAAACAGGCCAATGCAATGAATTACAAGAGGAAGAAAAAACATGGAGCTTGAACAACAAGGGTGCCCTTGTCCACGTCCATTCGGGCCTTGTCTTGACAGTACAAAATAGCACCAACACTGTGAATGTTGTGGCTTATAGAAATGTTCCATCACAAATTTGGAACGTCATAAACCGAGTCCCTAGCCACCGACGCGTTGGTCACATCATAGGACTTCATAGTTACGGGTGTTTAGTAATCTACTCGGATGCATTTTCGGTAATCAATTCGTTAAGTTGTACTAATAATCTTGAGACAAAACAATTGTGGGTTGTATATGAGGATGGTACAATTCGGGCAAATGCTAACGAAAGCATGTGCTTAACTTCGACTAATCATAAGGAGATCCCAGGACCTTATTCTATGATATTTGCAGTTCCATGCATGCAAGGCAAAGTGGAGCAACAGTGGGAGATGAACGTACAGGATTTCACCATTCACCATCCTGATTCAACATTGGTGTTGGATGTTATTTTAGTTGGAAGGGAGCCATATACACGAAAAATGGTTACTCTCGTTACACGTAATGGTGCTGCTACACAACAATGGACGTTTATGGAATGA

mRNA sequence

ATGAAAGTCCTGAAGACATACATCTTAGTAAAAACAGGCCAATGCAATGAATTACAAGAGGAAGAAAAAACATGGAGCTTGAACAACAAGGGTGCCCTTGTCCACGTCCATTCGGGCCTTGTCTTGACAGTACAAAATAGCACCAACACTGTGAATGTTGTGGCTTATAGAAATGTTCCATCACAAATTTGGAACGTCATAAACCGAGTCCCTAGCCACCGACGCGTTGGTCACATCATAGGACTTCATAGTTACGGGTGTTTAGTAATCTACTCGGATGCATTTTCGGTAATCAATTCGTTAAGTTGTACTAATAATCTTGAGACAAAACAATTGTGGGTTGTATATGAGGATGGTACAATTCGGGCAAATGCTAACGAAAGCATGTGCTTAACTTCGACTAATCATAAGGAGATCCCAGGACCTTATTCTATGATATTTGCAGTTCCATGCATGCAAGGCAAAGTGGAGCAACAGTGGGAGATGAACGTACAGGATTTCACCATTCACCATCCTGATTCAACATTGGTGTTGGATGTTATTTTAGTTGGAAGGGAGCCATATACACGAAAAATGGTTACTCTCGTTACACGTAATGGTGCTGCTACACAACAATGGACGTTTATGGAATGA

Coding sequence (CDS)

ATGAAAGTCCTGAAGACATACATCTTAGTAAAAACAGGCCAATGCAATGAATTACAAGAGGAAGAAAAAACATGGAGCTTGAACAACAAGGGTGCCCTTGTCCACGTCCATTCGGGCCTTGTCTTGACAGTACAAAATAGCACCAACACTGTGAATGTTGTGGCTTATAGAAATGTTCCATCACAAATTTGGAACGTCATAAACCGAGTCCCTAGCCACCGACGCGTTGGTCACATCATAGGACTTCATAGTTACGGGTGTTTAGTAATCTACTCGGATGCATTTTCGGTAATCAATTCGTTAAGTTGTACTAATAATCTTGAGACAAAACAATTGTGGGTTGTATATGAGGATGGTACAATTCGGGCAAATGCTAACGAAAGCATGTGCTTAACTTCGACTAATCATAAGGAGATCCCAGGACCTTATTCTATGATATTTGCAGTTCCATGCATGCAAGGCAAAGTGGAGCAACAGTGGGAGATGAACGTACAGGATTTCACCATTCACCATCCTGATTCAACATTGGTGTTGGATGTTATTTTAGTTGGAAGGGAGCCATATACACGAAAAATGGTTACTCTCGTTACACGTAATGGTGCTGCTACACAACAATGGACGTTTATGGAATGA

Protein sequence

MKVLKTYILVKTGQCNELQEEEKTWSLNNKGALVHVHSGLVLTVQNSTNTVNVVAYRNVPSQIWNVINRVPSHRRVGHIIGLHSYGCLVIYSDAFSVINSLSCTNNLETKQLWVVYEDGTIRANANESMCLTSTNHKEIPGPYSMIFAVPCMQGKVEQQWEMNVQDFTIHHPDSTLVLDVILVGREPYTRKMVTLVTRNGAATQQWTFME
Homology
[Close]
BLAST of Lsi05G001810 vs. Swiss-Prot
Match: RIP1_SAMNI (Ribosome-inactivating protein SNAI' OS=Sambucus nigra PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.8e-10
Identity = 63/208 (30.29%), Postives = 89/208 (42.79%), Query Frame = 1 

Query: 2   KVLKTYILVKTGQCNELQEEEKTWSLNNKGALVHVHSGLVLTVQNSTNTVNVVAYRNVPS 61
           K L T   V    C  +  E   W ++  G + +  SGLVLT   +     V   +NV +
Sbjct: 368 KCLTTSSSVMIYNCKVVPPESTKWVVSIDGTITNPRSGLVLTAPKAAEGTLVSLEKNVHA 427

Query: 62  --QIWNVINRVPSHRRVGHIIGLHSYGCLVIYSDAFSV-INSLSCTNNLETKQLWVVYED 121
             Q W V N  P    V  I+G     CL        V +   S  +  +  Q W +Y D
Sbjct: 428 ARQGWIVGNVEPL---VTFIVGYEQM-CLETNPGNNDVSLGDCSVKSASKVDQKWALYGD 487

Query: 122 GTIRANANESMCLTSTNHKEIPGPYSMIFAVPCMQGKVEQQWEMNVQDFTIHHPDSTLVL 181
           GTIR N + S+C+TS    E       I  + C+ G   Q+W  N  D TI +PDS LV+
Sbjct: 488 GTIRVNNDRSLCVTS----EGKSSNEPIIILKCL-GWANQRWVFNT-DGTISNPDSKLVM 547

Query: 182 DVILVGREPYTRKMVTLVTRNGAATQQW 207
               V +     + + L   +G + QQW
Sbjct: 548 H---VDQNDVPLRKIILSHPSGTSNQQW 562

[Close]
BLAST of Lsi05G001810 vs. Swiss-Prot
Match: ABRB_ABRPR (Abrin-b OS=Abrus precatorius PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.2e-08
Identity = 51/194 (26.29%), Postives = 86/194 (44.33%), Query Frame = 1 

Query: 15  CNELQEEEKTWSLNNKGALVHVHSGLVLTVQNST--NTVNVVAYRNVPSQIWNVINRVPS 74
           C     E   W + + G +++  S LVL+ ++S+   T+ V     +  Q W   N    
Sbjct: 345 CTSAVAEATYWEIWDNGTIINPKSALVLSAESSSMGGTLTVQTNEYLMRQGWRTGNNTSP 404

Query: 75  HRRVGHIIGLHSYGCLVIYSDAFSVINSLSCTNNLETKQLWVVYEDGTIRANANESMCLT 134
                 +  +  Y  L + +   +V  +  C NN + +Q W +Y DG+IR+  N + CLT
Sbjct: 405 F-----VTSISGYSDLCMQAQGSNVWLAY-CDNN-KKEQQWALYTDGSIRSVQNTNNCLT 464

Query: 135 STNHKEIPGPYSMIFAVPCMQGKVEQQWEMNVQDFTIHHPDSTLVLDVILVGREPYTRKM 194
           S +HK+     S I  + C  G   Q+W     D +I++    +V+D   V R   + K 
Sbjct: 465 SKDHKQ----GSPIVLMACSNGWASQRWLFR-NDGSIYNLHDDMVMD---VKRSDPSLKE 523

Query: 195 VTLVTRNGAATQQW 207
           + L   +G   Q W
Sbjct: 525 IILHPYHGKPNQIW 523

[Close]
BLAST of Lsi05G001810 vs. Swiss-Prot
Match: ABRC_ABRPR (Abrin-c OS=Abrus precatorius PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.2e-08
Identity = 51/194 (26.29%), Postives = 87/194 (44.85%), Query Frame = 1 

Query: 15  CNELQEEEKTWSLNNKGALVHVHSGLVLTVQNST--NTVNVVAYRNVPSQIWNVINRVPS 74
           C     E   W + + G +++  S LVL+ ++S+   T+ V     +  Q W   N    
Sbjct: 380 CTSAVAEATYWEIWDNGTIINPKSALVLSAESSSMGGTLTVQTNEYLMRQGWRTGNNTSP 439

Query: 75  HRRVGHIIGLHSYGCLVIYSDAFSVINSLSCTNNLETKQLWVVYEDGTIRANANESMCLT 134
                 +  +  Y  L + +   +V  +  C NN + +Q W +Y DG+IR+  N + CLT
Sbjct: 440 F-----VTSISGYSDLCMQAQGSNVWLA-DCDNN-KKEQQWALYTDGSIRSVQNTNNCLT 499

Query: 135 STNHKEIPGPYSMIFAVPCMQGKVEQQWEMNVQDFTIHHPDSTLVLDVILVGREPYTRKM 194
           S +HK+     S I  + C  G   Q+W +   D +I++    +V+D   V R   + K 
Sbjct: 500 SKDHKQ----GSPIVLMACSNGWASQRW-LFKNDGSIYNLHDDMVMD---VKRSDPSLKE 558

Query: 195 VTLVTRNGAATQQW 207
           + L   +G   Q W
Sbjct: 560 IILHPYHGKPNQIW 558

[Close]
BLAST of Lsi05G001810 vs. Swiss-Prot
Match: ABRD_ABRPR (Abrin-d OS=Abrus precatorius PE=2 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.5e-08
Identity = 52/194 (26.80%), Postives = 87/194 (44.85%), Query Frame = 1 

Query: 15  CNELQEEEKTWSLNNKGALVHVHSGLVLTVQNST--NTVNVVAYRNVPSQIWNVINRVPS 74
           C     E   W + + G +++  S LVL+ ++S+   T+ V     +  Q W   N    
Sbjct: 346 CTSAVAEATYWEIWDNGTIINPKSALVLSAESSSMGGTLTVQTNEYLMRQGWRTGNNTSP 405

Query: 75  HRRVGHIIGLHSYGCLVIYSDAFSVINSLSCTNNLETKQLWVVYEDGTIRANANESMCLT 134
                 +  +  Y  L + +   +V  +  C NN + +Q W +Y DG+IR+  N + CLT
Sbjct: 406 F-----VTSISGYSDLCMQAQGSNVWLA-DCDNN-KKEQQWALYTDGSIRSVQNTNNCLT 465

Query: 135 STNHKEIPGPYSMIFAVPCMQGKVEQQWEMNVQDFTIHHPDSTLVLDVILVGREPYTRKM 194
           S +HK+     S I  + C  G   Q+W +   D +I+     +V+DV   G +P + K 
Sbjct: 466 SKDHKQ----GSPIVLMACSNGWASQRW-LFKNDGSIYSLYDDMVMDV--KGSDP-SLKQ 524

Query: 195 VTLVTRNGAATQQW 207
           + L    G   Q W
Sbjct: 526 IILWPYTGKPNQIW 524

[Close]
BLAST of Lsi05G001810 vs. Swiss-Prot
Match: ABRA_ABRPR (Abrin-a OS=Abrus precatorius PE=1 SV=2)

HSP 1 Score: 60.8 bits (146), Expect = 2.0e-08
Identity = 52/203 (25.62%), Postives = 91/203 (44.83%), Query Frame = 1 

Query: 6   TYILVKTGQCNELQEEEKTWSLNNKGALVHVHSGLVLTVQNST--NTVNVVAYRNVPSQI 65
           +Y+++    C     E   W + + G +++  S LVL+ ++S+   T+ V     +  Q 
Sbjct: 339 SYVMIY--DCTSAVAEATYWEIWDNGTIINPKSALVLSAESSSMGGTLTVQTNEYLMRQG 398

Query: 66  WNVINRVPSHRRVGHIIGLHSYGCLVIYSDAFSVINSLSCTNNLETKQLWVVYEDGTIRA 125
           W   N          +  +  Y  L + +   +V  +  C +N + +Q W +Y DG+IR+
Sbjct: 399 WRTGNNTSPF-----VTSISGYSDLCMQAQGSNVWMA-DCDSN-KKEQQWALYTDGSIRS 458

Query: 126 NANESMCLTSTNHKEIPGPYSMIFAVPCMQGKVEQQWEMNVQDFTIHHPDSTLVLDVILV 185
             N + CLTS +HK+     S I  + C  G   Q+W     D +I+     +V+DV   
Sbjct: 459 VQNTNNCLTSKDHKQ----GSTILLMGCSNGWASQRWVFK-NDGSIYSLYDDMVMDV--K 518

Query: 186 GREPYTRKMVTLVTRNGAATQQW 207
           G +P + K + L    G   Q W
Sbjct: 519 GSDP-SLKQIILWPYTGKPNQIW 524

[Close]
BLAST of Lsi05G001810 vs. TrEMBL
Match: A0A0A0L9D1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217160 PE=4 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 9.0e-16
Identity = 61/196 (31.12%), Postives = 100/196 (51.02%), Query Frame = 1 

Query: 14  QCNELQEEEKTWSLNNKGALVHVHSGLVLTVQNSTNTVNVVAYRNVPSQIWNVINRVPSH 73
           +C ++ +  K W+  N G +  V S +VLT     + V + + +  PSQ W V   + S 
Sbjct: 111 ECGKVSDPNKKWTQKNDGTIALVDSRMVLT--GDLDYVTLQSNKYTPSQSWEVTESLNS- 170

Query: 74  RRVGHIIGLHSYGCLVIYSDAFSVINSLSCTNNLETKQLWVVYEDGTIRANANESMCLTS 133
             V +I  L++  CL    D+  V   L+  N     Q W +Y DGTIR + N++ CLTS
Sbjct: 171 -MVANIEWLNNL-CLQSTDDSSHV--GLNGCNTDNKYQRWALYADGTIRQHVNKNYCLTS 230

Query: 134 TNHKEIPGPYSMIFAVPCMQGKVEQQWEMNVQDFTIHHPDSTLVLDVILVGREPYTRKMV 193
                    +     V   + K +Q+W ++ +D+TI HP++ +VLDV  V    +   +V
Sbjct: 231 DQ------DFGRFVVVSKCEDKPQQRWSLDAKDYTIDHPNTDMVLDVFSVPDSTFPSVLV 290

Query: 194 TLVTRNGAATQQWTFM 210
           T   R+G+A+Q+WT +
Sbjct: 291 T-NRRDGSASQRWTII 292

[Close]
BLAST of Lsi05G001810 vs. TrEMBL
Match: Q9SLV9_CUCSA (XSP30 OS=Cucumis sativus GN=XSP30 PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 9.0e-16
Identity = 61/196 (31.12%), Postives = 100/196 (51.02%), Query Frame = 1 

Query: 14  QCNELQEEEKTWSLNNKGALVHVHSGLVLTVQNSTNTVNVVAYRNVPSQIWNVINRVPSH 73
           +C ++ +  K W+  N G +  V S +VLT     + V + + +  PSQ W V   + S 
Sbjct: 111 ECGKVSDPNKKWTQKNDGTIALVDSRMVLT--GDLDYVTLQSNKYTPSQSWEVTESLNS- 170

Query: 74  RRVGHIIGLHSYGCLVIYSDAFSVINSLSCTNNLETKQLWVVYEDGTIRANANESMCLTS 133
             V +I  L++  CL    D+  V   L+  N     Q W +Y DGTIR + N++ CLTS
Sbjct: 171 -MVANIEWLNNL-CLQSTDDSSHV--GLNGCNTDNKYQRWALYADGTIRQHVNKNYCLTS 230

Query: 134 TNHKEIPGPYSMIFAVPCMQGKVEQQWEMNVQDFTIHHPDSTLVLDVILVGREPYTRKMV 193
                    +     V   + K +Q+W ++ +D+TI HP++ +VLDV  V    +   +V
Sbjct: 231 DQ------DFGRFVVVSKCEDKPQQRWSLDAKDYTIDHPNTDMVLDVFSVPDSTFPSVLV 290

Query: 194 TLVTRNGAATQQWTFM 210
           T   R+G+A+Q+WT +
Sbjct: 291 T-NRRDGSASQRWTII 292

[Close]
BLAST of Lsi05G001810 vs. TrEMBL
Match: A0A0A0L756_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G219180 PE=4 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 3.4e-15
Identity = 65/199 (32.66%), Postives = 97/199 (48.74%), Query Frame = 1 

Query: 10  VKTGQCNELQEEEKTWSLNNKGALVHVHSGLVLTVQNSTNTVNVVAYRNVPSQIWNVINR 69
           V   +C +     K W       +VHV SG+VLT   ++N V V   +N PSQ W     
Sbjct: 103 VVVSECAKEPHSNKKWIHKEDRTIVHVGSGMVLT--GNSNYVTVQRNKNAPSQSWEATKS 162

Query: 70  VPSHRRVGHIIGLHSYGCLVIYSDAFSVINSLSCTNNLETKQLWVVYEDGTIRANANESM 129
           +     V +I  L +  CL    D+  V   L   N+    Q W +Y DGTIR + N + 
Sbjct: 163 LTP--MVANIKWLENL-CLQSTKDSNYV--QLDGCNSENKSQHWSLYGDGTIRKHVNRNY 222

Query: 130 CLTSTNHKEIPGPYSMIFAVPCMQGKVEQQWEMNVQDFTIHHPDSTLVLDVILVGREPYT 189
           CLTS         +    AV   + K +Q+W +  +D TI+HP++ +V+DV+ V   P+ 
Sbjct: 223 CLTSEQ------DFGRFVAVSKCEDKPQQRWGLGAEDNTINHPNTDMVMDVLSV---PFD 282

Query: 190 RKMVTLVT--RNGAATQQW 207
              + +VT  R+G ATQ+W
Sbjct: 283 DLPLAVVTNHRDGTATQRW 285

[Close]
BLAST of Lsi05G001810 vs. TrEMBL
Match: A0A0A0LAC2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217140 PE=4 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.2e-14
Identity = 59/194 (30.41%), Postives = 97/194 (50.00%), Query Frame = 1 

Query: 14  QCNELQEEEKTWSLNNKGALVHVHSGLVLTVQNSTNTVNVVAYRNVPSQIWNVINRVPSH 73
           +C ++ +  K W   N G +    S +VLT  +   T+  + Y   PSQ W V   +  +
Sbjct: 113 ECGKVSDSNKQWIHKNDGTIALAESRMVLTGDSDYVTLQSIKY--TPSQSWEVTESL--N 172

Query: 74  RRVGHIIGLHSYGCLVIYSDAFSVINSLSCTNNLETKQLWVVYEDGTIRANANESMCLTS 133
             V +I  L++  CL    D+ +V   L+  N     Q W +Y DGTIR + N + CLTS
Sbjct: 173 PMVANIEWLNNL-CLQSTDDSSNV--GLNGCNTYTKYQRWALYADGTIRQHVNRNYCLTS 232

Query: 134 TNHKEIPGPYSMIFAVPCMQGKVEQQWEMNVQDFTIHHPDSTLVLDVILVGREPYTRKMV 193
             +      Y     V   + K +Q+W +N +D TI HP++ +VLDV+ V  +  +  ++
Sbjct: 233 EQN------YDRSVVVSKCEDKPQQRWSLNAKDSTIDHPNTNMVLDVLNV-LDSASPLVI 292

Query: 194 TLVTRNGAATQQWT 208
                +G+A+Q+WT
Sbjct: 293 VTNHPDGSASQRWT 292

[Close]
BLAST of Lsi05G001810 vs. TrEMBL
Match: B5WYH8_SAMEB (rRNA N-glycosylase (Fragment) OS=Sambucus ebulus GN=ar PE=4 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.3e-11
Identity = 62/208 (29.81%), Postives = 96/208 (46.15%), Query Frame = 1 

Query: 2   KVLKTYILVKTGQCNELQEEEKTWSLNNKGALVHVHSGLVLTVQNSTNTVNVVAYRNVPS 61
           K L     V    CN +  E+  W ++  G + +  SGLVLT   +     +    N+ +
Sbjct: 341 KCLTASSSVMINDCNTVPPEDTKWVVSIDGTITNPSSGLVLTAPQTLEGTALSPENNIHA 400

Query: 62  QI--WNVINRVPSHRRVGHIIGLHSYGCLVIYSDAFSVINSLS-CTNNLETKQLWVVYED 121
            I  W V +  P    V  I+G   Y  + +  D  +    +  C  N  T+Q W +Y D
Sbjct: 401 AIQGWTVGDVEPL---VTSIVG---YKQMCLREDGENNFVWMEHCVLN-RTEQEWALYGD 460

Query: 122 GTIRANANESMCLTSTNHKEIPGPYSMIFAVPCMQGKVEQQWEMNVQDFTIHHPDSTLVL 181
           GTIR N+N S+C+TS  H+    P  +I  + C QG   Q+W  N    TI +P++T V+
Sbjct: 461 GTIRVNSNRSLCVTSQYHE----PSDLIIILKC-QGSSNQRWVFNTNG-TISNPNTTKVM 520

Query: 182 DVILVGREPYTRKMVTLVTRNGAATQQW 207
           D   V +   + + + L   NG +  QW
Sbjct: 521 D---VAQANVSLRKIILYRPNGESNHQW 532

[Close]
BLAST of Lsi05G001810 vs. NCBI nr
Match: gi|659112133|ref|XP_008456079.1| (PREDICTED: nigrin b-like [Cucumis melo])

HSP 1 Score: 94.7 bits (234), Expect = 2.0e-16
Identity = 61/194 (31.44%), Postives = 97/194 (50.00%), Query Frame = 1 

Query: 14  QCNELQEEEKTWSLNNKGALVHVHSGLVLTVQNSTNTVNVVAYRNVPSQIWNVINRVPSH 73
           +C +     K W+    G + HV SG+VLT    ++ V + + +N PSQ W       S 
Sbjct: 111 ECAKEPNSNKRWTHKKDGTITHVESGMVLT--GDSDYVTLQSNKNTPSQSWEATES--ST 170

Query: 74  RRVGHIIGLHSYGCLVIYSDAFSVINSLSCTNNLETKQLWVVYEDGTIRANANESMCLTS 133
             + +I  L++  CL    D+  V   L+  N     Q W +Y DGTIR N N + CLTS
Sbjct: 171 PMIANIEWLNNL-CLQSTDDSNHV--GLNGCNRENKDQRWALYGDGTIRQNVNRNYCLTS 230

Query: 134 TNHKEIPGPYSMIFAVPCMQGKVEQQWEMNVQDFTIHHPDSTLVLDVILVGREPYTRKMV 193
             +            +   + K +Q+W +  +D TI HP+S +V+DV+ V    ++  +V
Sbjct: 231 DEY------LGRFIVMSKCEDKPQQRWNLGAKDSTIDHPNSDMVMDVLFVPYYDFSSAVV 290

Query: 194 TLVTRNGAATQQWT 208
           T   R+G+A+Q+WT
Sbjct: 291 T-NRRDGSASQRWT 290

[Close]
BLAST of Lsi05G001810 vs. NCBI nr
Match: gi|700202446|gb|KGN57579.1| (hypothetical protein Csa_3G217160 [Cucumis sativus])

HSP 1 Score: 92.0 bits (227), Expect = 1.3e-15
Identity = 61/196 (31.12%), Postives = 100/196 (51.02%), Query Frame = 1 

Query: 14  QCNELQEEEKTWSLNNKGALVHVHSGLVLTVQNSTNTVNVVAYRNVPSQIWNVINRVPSH 73
           +C ++ +  K W+  N G +  V S +VLT     + V + + +  PSQ W V   + S 
Sbjct: 111 ECGKVSDPNKKWTQKNDGTIALVDSRMVLT--GDLDYVTLQSNKYTPSQSWEVTESLNS- 170

Query: 74  RRVGHIIGLHSYGCLVIYSDAFSVINSLSCTNNLETKQLWVVYEDGTIRANANESMCLTS 133
             V +I  L++  CL    D+  V   L+  N     Q W +Y DGTIR + N++ CLTS
Sbjct: 171 -MVANIEWLNNL-CLQSTDDSSHV--GLNGCNTDNKYQRWALYADGTIRQHVNKNYCLTS 230

Query: 134 TNHKEIPGPYSMIFAVPCMQGKVEQQWEMNVQDFTIHHPDSTLVLDVILVGREPYTRKMV 193
                    +     V   + K +Q+W ++ +D+TI HP++ +VLDV  V    +   +V
Sbjct: 231 DQ------DFGRFVVVSKCEDKPQQRWSLDAKDYTIDHPNTDMVLDVFSVPDSTFPSVLV 290

Query: 194 TLVTRNGAATQQWTFM 210
           T   R+G+A+Q+WT +
Sbjct: 291 T-NRRDGSASQRWTII 292

[Close]
BLAST of Lsi05G001810 vs. NCBI nr
Match: gi|566006087|ref|NP_001274403.1| (uncharacterized protein LOC101221335 precursor [Cucumis sativus])

HSP 1 Score: 92.0 bits (227), Expect = 1.3e-15
Identity = 61/196 (31.12%), Postives = 100/196 (51.02%), Query Frame = 1 

Query: 14  QCNELQEEEKTWSLNNKGALVHVHSGLVLTVQNSTNTVNVVAYRNVPSQIWNVINRVPSH 73
           +C ++ +  K W+  N G +  V S +VLT     + V + + +  PSQ W V   + S 
Sbjct: 111 ECGKVSDPNKKWTQKNDGTIALVDSRMVLT--GDLDYVTLQSNKYTPSQSWEVTESLNS- 170

Query: 74  RRVGHIIGLHSYGCLVIYSDAFSVINSLSCTNNLETKQLWVVYEDGTIRANANESMCLTS 133
             V +I  L++  CL    D+  V   L+  N     Q W +Y DGTIR + N++ CLTS
Sbjct: 171 -MVANIEWLNNL-CLQSTDDSSHV--GLNGCNTDNKYQRWALYADGTIRQHVNKNYCLTS 230

Query: 134 TNHKEIPGPYSMIFAVPCMQGKVEQQWEMNVQDFTIHHPDSTLVLDVILVGREPYTRKMV 193
                    +     V   + K +Q+W ++ +D+TI HP++ +VLDV  V    +   +V
Sbjct: 231 DQ------DFGRFVVVSKCEDKPQQRWSLDAKDYTIDHPNTDMVLDVFSVPDSTFPSVLV 290

Query: 194 TLVTRNGAATQQWTFM 210
           T   R+G+A+Q+WT +
Sbjct: 291 T-NRRDGSASQRWTII 292

[Close]
BLAST of Lsi05G001810 vs. NCBI nr
Match: gi|449457105|ref|XP_004146289.1| (PREDICTED: nigrin b-like [Cucumis sativus])

HSP 1 Score: 90.1 bits (222), Expect = 4.9e-15
Identity = 65/199 (32.66%), Postives = 97/199 (48.74%), Query Frame = 1 

Query: 10  VKTGQCNELQEEEKTWSLNNKGALVHVHSGLVLTVQNSTNTVNVVAYRNVPSQIWNVINR 69
           V   +C +     K W       +VHV SG+VLT   ++N V V   +N PSQ W     
Sbjct: 103 VVVSECAKEPHSNKKWIHKEDRTIVHVGSGMVLT--GNSNYVTVQRNKNAPSQSWEATKS 162

Query: 70  VPSHRRVGHIIGLHSYGCLVIYSDAFSVINSLSCTNNLETKQLWVVYEDGTIRANANESM 129
           +     V +I  L +  CL    D+  V   L   N+    Q W +Y DGTIR + N + 
Sbjct: 163 LTP--MVANIKWLENL-CLQSTKDSNYV--QLDGCNSENKSQHWSLYGDGTIRKHVNRNY 222

Query: 130 CLTSTNHKEIPGPYSMIFAVPCMQGKVEQQWEMNVQDFTIHHPDSTLVLDVILVGREPYT 189
           CLTS         +    AV   + K +Q+W +  +D TI+HP++ +V+DV+ V   P+ 
Sbjct: 223 CLTSEQ------DFGRFVAVSKCEDKPQQRWGLGAEDNTINHPNTDMVMDVLSV---PFD 282

Query: 190 RKMVTLVT--RNGAATQQW 207
              + +VT  R+G ATQ+W
Sbjct: 283 DLPLAVVTNHRDGTATQRW 285

[Close]
BLAST of Lsi05G001810 vs. NCBI nr
Match: gi|778680239|ref|XP_004151509.2| (PREDICTED: nigrin b-like [Cucumis sativus])

HSP 1 Score: 87.4 bits (215), Expect = 3.2e-14
Identity = 59/194 (30.41%), Postives = 97/194 (50.00%), Query Frame = 1 

Query: 14  QCNELQEEEKTWSLNNKGALVHVHSGLVLTVQNSTNTVNVVAYRNVPSQIWNVINRVPSH 73
           +C ++ +  K W   N G +    S +VLT  +   T+  + Y   PSQ W V   +  +
Sbjct: 113 ECGKVSDSNKQWIHKNDGTIALAESRMVLTGDSDYVTLQSIKY--TPSQSWEVTESL--N 172

Query: 74  RRVGHIIGLHSYGCLVIYSDAFSVINSLSCTNNLETKQLWVVYEDGTIRANANESMCLTS 133
             V +I  L++  CL    D+ +V   L+  N     Q W +Y DGTIR + N + CLTS
Sbjct: 173 PMVANIEWLNNL-CLQSTDDSSNV--GLNGCNTYTKYQRWALYADGTIRQHVNRNYCLTS 232

Query: 134 TNHKEIPGPYSMIFAVPCMQGKVEQQWEMNVQDFTIHHPDSTLVLDVILVGREPYTRKMV 193
             +      Y     V   + K +Q+W +N +D TI HP++ +VLDV+ V  +  +  ++
Sbjct: 233 EQN------YDRSVVVSKCEDKPQQRWSLNAKDSTIDHPNTNMVLDVLNV-LDSASPLVI 292

Query: 194 TLVTRNGAATQQWT 208
                +G+A+Q+WT
Sbjct: 293 VTNHPDGSASQRWT 292

The following BLAST results are available for this feature:
BLAST of Lsi05G001810 vs. Swiss-Prot
Match NameE-valueIdentityDescription
RIP1_SAMNI2.8e-1030.29Ribosome-inactivating protein SNAI' OS=Sambucus nigra PE=1 SV=1[more]
ABRB_ABRPR1.2e-0826.29Abrin-b OS=Abrus precatorius PE=1 SV=1[more]
ABRC_ABRPR1.2e-0826.29Abrin-c OS=Abrus precatorius PE=1 SV=1[more]
ABRD_ABRPR1.5e-0826.80Abrin-d OS=Abrus precatorius PE=2 SV=1[more]
ABRA_ABRPR2.0e-0825.62Abrin-a OS=Abrus precatorius PE=1 SV=2[more]
BLAST of Lsi05G001810 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L9D1_CUCSA9.0e-1631.12Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217160 PE=4 SV=1[more]
Q9SLV9_CUCSA9.0e-1631.12XSP30 OS=Cucumis sativus GN=XSP30 PE=2 SV=1[more]
A0A0A0L756_CUCSA3.4e-1532.66Uncharacterized protein OS=Cucumis sativus GN=Csa_3G219180 PE=4 SV=1[more]
A0A0A0LAC2_CUCSA2.2e-1430.41Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217140 PE=4 SV=1[more]
B5WYH8_SAMEB2.3e-1129.81rRNA N-glycosylase (Fragment) OS=Sambucus ebulus GN=ar PE=4 SV=1[more]
BLAST of Lsi05G001810 vs. TAIR10
Match NameE-valueIdentityDescription
BLAST of Lsi05G001810 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659112133|ref|XP_008456079.1|2.0e-1631.44PREDICTED: nigrin b-like [Cucumis melo][more]
gi|700202446|gb|KGN57579.1|1.3e-1531.12hypothetical protein Csa_3G217160 [Cucumis sativus][more]
gi|566006087|ref|NP_001274403.1|1.3e-1531.12uncharacterized protein LOC101221335 precursor [Cucumis sativus][more]
gi|449457105|ref|XP_004146289.1|4.9e-1532.66PREDICTED: nigrin b-like [Cucumis sativus][more]
gi|778680239|ref|XP_004151509.2|3.2e-1430.41PREDICTED: nigrin b-like [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000772Ricin_B_lectin
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G001810.1Lsi05G001810.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000772Ricin B, lectin domainPFAMPF00652Ricin_B_lectincoord: 76..206
score: 8.8
IPR000772Ricin B, lectin domainSMARTSM00458ricin_3coord: 74..209
score: 1.
IPR000772Ricin B, lectin domainPROFILEPS50231RICIN_B_LECTINcoord: 87..209
score: 22.122coord: 1..67
score: 10
IPR000772Ricin B, lectin domainunknownSSF50370Ricin B-like lectinscoord: 76..208
score: 7.13E-19coord: 10..66
score: 2.3
NoneNo IPR availableGENE3DG3DSA:2.80.10.50coord: 11..66
score: 2.1E-6coord: 87..209
score: 2.1