CmaCh04G005230 (gene) Cucurbita maxima (Rimu)

NameCmaCh04G005230
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionrRNA N-glycosidase
LocationCma_Chr04 : 2644555 .. 2645412 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAGATGAGACAAGTCCTGCTCTGCATTGCCCTGGCATTCTCCCTCGCCATAGCGGCGCCCTCTGAGTACGGAGCGTTCGGAGGACGCACACATCTTGTTGGTCGAGATGGGCTGTGTTTAGACACCATGTTGCTTTCCGGATATTATCCTCAAACTGACTTATCCCAATGCCAACCAAGGAAACTGAGCCAGCTATGGACCATCCTTGAAGATGGCACGATTCGAGCCAGTGATGTTAGATACTGTTTGGTTCCTAAAACTCAATTCATTGGACCCGTTGGACCCAATGTCGTAGTAATGGACTGCACAAAAGAGGTAAGACACGACATGAAATGGACCAAAAAGAAGGATGGAACCATACACCACGATGAGTCGGGGCTTGTCCTGACGGCGAAACCAGGCAGGATGGTGACATTGGAAGCCAATGAAGACGCGCCATCACAAAGCTGGGAAGCCACGGAGAGCCTCTACCCAATGGTGGCTAATATCAAATGGCTTGATAACTTGTGTTTGAAATCTAGAGAAGGTGAAAATTCGTATGTGACGTTGAACGAGTGTAGCCGAGATGATAAGAACCAACGATGGGCGTTGTATGGGGACGGCACCATTCGACAAAATGAGTATAGACACTTGTGCTTGACTTCGTCCGAAAGGAATTATGGTGCGCTGGTGGTTGTGTCTAGGTGCGCTGACAAGCCACAGCAGCGTTGGGGGCTTGCGGAAGACAACACCATCAACCATCCCAACACCAACTTGGTCATGGATGTGCATAGGGAGGTGCCTCTGTTGCCGCCCATGATCGTGGTTGACAAGCATGACGGCGCTCCTAGCCAACGATGGTCCATTTACTGA

mRNA sequence

ATGGAGAAGATGAGACAAGTCCTGCTCTGCATTGCCCTGGCATTCTCCCTCGCCATAGCGGCGCCCTCTGAGTACGGAGCGTTCGGAGGACGCACACATCTTGTTGGTCGAGATGGGCTGTGTTTAGACACCATGTTGCTTTCCGGATATTATCCTCAAACTGACTTATCCCAATGCCAACCAAGGAAACTGAGCCAGCTATGGACCATCCTTGAAGATGGCACGATTCGAGCCAGTGATGTTAGATACTGTTTGGTTCCTAAAACTCAATTCATTGGACCCGTTGGACCCAATGTCGTAGTAATGGACTGCACAAAAGAGGTAAGACACGACATGAAATGGACCAAAAAGAAGGATGGAACCATACACCACGATGAGTCGGGGCTTGTCCTGACGGCGAAACCAGGCAGGATGGTGACATTGGAAGCCAATGAAGACGCGCCATCACAAAGCTGGGAAGCCACGGAGAGCCTCTACCCAATGGTGGCTAATATCAAATGGCTTGATAACTTGTGTTTGAAATCTAGAGAAGGTGAAAATTCGTATGTGACGTTGAACGAGTGTAGCCGAGATGATAAGAACCAACGATGGGCGTTGTATGGGGACGGCACCATTCGACAAAATGAGTATAGACACTTGTGCTTGACTTCGTCCGAAAGGAATTATGGTGCGCTGGTGGTTGTGTCTAGGTGCGCTGACAAGCCACAGCAGCGTTGGGGGCTTGCGGAAGACAACACCATCAACCATCCCAACACCAACTTGGTCATGGATGTGCATAGGGAGGTGCCTCTGTTGCCGCCCATGATCGTGGTTGACAAGCATGACGGCGCTCCTAGCCAACGATGGTCCATTTACTGA

Coding sequence (CDS)

ATGGAGAAGATGAGACAAGTCCTGCTCTGCATTGCCCTGGCATTCTCCCTCGCCATAGCGGCGCCCTCTGAGTACGGAGCGTTCGGAGGACGCACACATCTTGTTGGTCGAGATGGGCTGTGTTTAGACACCATGTTGCTTTCCGGATATTATCCTCAAACTGACTTATCCCAATGCCAACCAAGGAAACTGAGCCAGCTATGGACCATCCTTGAAGATGGCACGATTCGAGCCAGTGATGTTAGATACTGTTTGGTTCCTAAAACTCAATTCATTGGACCCGTTGGACCCAATGTCGTAGTAATGGACTGCACAAAAGAGGTAAGACACGACATGAAATGGACCAAAAAGAAGGATGGAACCATACACCACGATGAGTCGGGGCTTGTCCTGACGGCGAAACCAGGCAGGATGGTGACATTGGAAGCCAATGAAGACGCGCCATCACAAAGCTGGGAAGCCACGGAGAGCCTCTACCCAATGGTGGCTAATATCAAATGGCTTGATAACTTGTGTTTGAAATCTAGAGAAGGTGAAAATTCGTATGTGACGTTGAACGAGTGTAGCCGAGATGATAAGAACCAACGATGGGCGTTGTATGGGGACGGCACCATTCGACAAAATGAGTATAGACACTTGTGCTTGACTTCGTCCGAAAGGAATTATGGTGCGCTGGTGGTTGTGTCTAGGTGCGCTGACAAGCCACAGCAGCGTTGGGGGCTTGCGGAAGACAACACCATCAACCATCCCAACACCAACTTGGTCATGGATGTGCATAGGGAGGTGCCTCTGTTGCCGCCCATGATCGTGGTTGACAAGCATGACGGCGCTCCTAGCCAACGATGGTCCATTTACTGA

Protein sequence

MEKMRQVLLCIALAFSLAIAAPSEYGAFGGRTHLVGRDGLCLDTMLLSGYYPQTDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKEVRHDMKWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKWLDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVVVSRCADKPQQRWGLAEDNTINHPNTNLVMDVHREVPLLPPMIVVDKHDGAPSQRWSIY
BLAST of CmaCh04G005230 vs. Swiss-Prot
Match: NIGB_SAMNI (Nigrin b OS=Sambucus nigra PE=1 SV=2)

HSP 1 Score: 147.5 bits (371), Expect = 2.2e-34
Identity = 91/255 (35.69%), Postives = 132/255 (51.76%), Query Frame = 1

Query: 33  HLVGRDGLCLDTMLLSGYYPQTDLSQCQP--RKLSQLWTILEDGTIRASDVRYCLVPKTQ 92
           ++VGRDGLC+D    +GY       Q  P   + +Q WT   D TIR+  +  C+     
Sbjct: 311 NIVGRDGLCVDVR--NGYDTDGTPLQLWPCGTQRNQRWTFDSDDTIRS--MGKCMTANGL 370

Query: 93  FIGPVGPNVVVMDCTKEVRHDMKWTKKKDGTIHHDESGLVLTAKPGRMVT---LEANEDA 152
                G N+V+ +C+    + +KW    DG+I +  SGLV+TA      T   LE N  A
Sbjct: 371 ---NNGSNIVIFNCSTAAENAIKWEVPIDGSIINPSSGLVMTAPRAASRTILLLEDNIYA 430

Query: 153 PSQSWEATESLYPMVANIKWLDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQ 212
            SQ W  T ++ P+VA+I     +CL+S  GEN+ V + +C      Q+WALYGD TIR 
Sbjct: 431 ASQGWTVTNNVKPIVASIVGYKEMCLQSN-GENNGVWMEDCEATSLQQQWALYGDRTIRV 490

Query: 213 NEYRHLCLTSSERNYGALVVVSRCADKPQQRWGLAEDNTINHPNTNLVMDVHREVPLLPP 272
           N  R LC+T++  N   L+++ +C   P QRW    D  I +P +  VMDV      L  
Sbjct: 491 NSTRGLCVTTNGYNSKDLIIILKCQGLPSQRWFFNSDGAIVNPKSRHVMDVRASNVSLRE 550

Query: 273 MIVVDKHDGAPSQRW 283
           +I+     G P+Q+W
Sbjct: 551 IIIFPA-TGNPNQQW 556

BLAST of CmaCh04G005230 vs. Swiss-Prot
Match: ABRC_ABRPR (Abrin-c OS=Abrus precatorius PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.1e-30
Identity = 87/254 (34.25%), Postives = 136/254 (53.54%), Query Frame = 1

Query: 36  GRDGLCLDTMLLSGYYPQTDLS--QCQPR-KLSQLWTILEDGTIRASDVRYCLVPKTQFI 95
           GRDG+C+D +   GY+    +   +C+ R + +QLWT+  D TIR++    CL   T+  
Sbjct: 315 GRDGMCVD-VYDDGYHNGNRIIAWKCKDRLEENQLWTLKSDKTIRSNGK--CLT--TEGY 374

Query: 96  GPVGPNVVVMDCTKEVRHDMKWTKKKDGTIHHDESGLVLTAKPGRM---VTLEANEDAPS 155
            P G  V++ DCT  V     W    +GTI + +S LVL+A+   M   +T++ NE    
Sbjct: 375 AP-GNYVMIYDCTSAVAEATYWEIWDNGTIINPKSALVLSAESSSMGGTLTVQTNEYLMR 434

Query: 156 QSWEATESLYPMVANIKWLDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNE 215
           Q W    +  P V +I    +LC+   + + S V L +C  + K Q+WALY DG+IR  +
Sbjct: 435 QGWRTGNNTSPFVTSISGYSDLCM---QAQGSNVWLADCDNNKKEQQWALYTDGSIRSVQ 494

Query: 216 YRHLCLTSSERNYGALVVVSRCADK-PQQRWGLAEDNTINHPNTNLVMDVHREVPLLPPM 275
             + CLTS +   G+ +V+  C++    QRW    D +I + + ++VMDV R  P L  +
Sbjct: 495 NTNNCLTSKDHKQGSPIVLMACSNGWASQRWLFKNDGSIYNLHDDMVMDVKRSDPSLKEI 554

Query: 276 IVVDKHDGAPSQRW 283
           I+   H G P+Q W
Sbjct: 555 ILHPYH-GKPNQIW 558

BLAST of CmaCh04G005230 vs. Swiss-Prot
Match: SGSL_TRIAN (Seed lectin (Fragments) OS=Trichosanthes anguina PE=1 SV=2)

HSP 1 Score: 133.3 bits (334), Expect = 4.3e-30
Identity = 85/262 (32.44%), Postives = 133/262 (50.76%), Query Frame = 1

Query: 32  THLVGRDGLCLDTM-LLSGYYPQTDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQ 91
           T + GRD LC+D    L+    +  L  C  ++++Q WT   DGT+R+  +  CL     
Sbjct: 265 TRISGRDALCVDVAGALTSDGSRLILYPCG-QQVNQKWTFHSDGTVRS--LGKCLATNNS 324

Query: 92  FIGPVGPNVVVMDCTKEVRHDMKWTKKKDGTIHHDE-SGLVLTAKPGRM---VTLEANED 151
             G +   VV+ DC+K    D+ W     GTI +     L LT+        +T+E N  
Sbjct: 325 KFGNL---VVIYDCSKLAAEDISWDVSVGGTIMNPNYEDLALTSNKATRSTNLTMEVNTY 384

Query: 152 APSQSWEATESLYPMVANIKWLDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIR 211
           + SQ W     + P++ +I  LD++CL++ +G N+ + L EC  + + Q WALY DGTIR
Sbjct: 385 SASQGWRVGNYVQPIIGSIVGLDDMCLEATDG-NTNMWLEECVPNQREQSWALYSDGTIR 444

Query: 212 QNEYRHLCLTSSERNYG--ALVVVSRCADKPQQRWGLAEDNTINHP-NTNLVMDVHREVP 271
            ++ R LC+T+S   Y    ++ +  C     QRW    D +I+ P N  L MDV R   
Sbjct: 445 VDDNRELCVTASSSTYDNWKVITILNCDGSNNQRWVFLADGSISTPGNQRLAMDVARSDV 504

Query: 272 LLPPMIVVDKHDGAPSQRWSIY 286
            L  +I+   H G  +Q+W ++
Sbjct: 505 DLKKIILHRPH-GDLNQQWVLF 518

BLAST of CmaCh04G005230 vs. Swiss-Prot
Match: RIP1_SAMNI (Ribosome-inactivating protein SNAI' OS=Sambucus nigra PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 7.3e-30
Identity = 88/257 (34.24%), Postives = 130/257 (50.58%), Query Frame = 1

Query: 36  GRDGLCLDTMLLSGYYPQ---TDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFI 95
           GRDG C +    +G         LS C  +  +Q WT   DGTI++  +  CL   +   
Sbjct: 323 GRDGFCAEVK--NGDEKDGTPVQLSSCGEQS-NQQWTFSTDGTIQS--LGKCLTTSS--- 382

Query: 96  GPVGPNVVVMDCTKEVRHDMKWTKKKDGTIHHDESGLVLTAKP---GRMVTLEANEDAPS 155
                +V++ +C        KW    DGTI +  SGLVLTA     G +V+LE N  A  
Sbjct: 383 -----SVMIYNCKVVPPESTKWVVSIDGTITNPRSGLVLTAPKAAEGTLVSLEKNVHAAR 442

Query: 156 QSWEATESLYPMVANIKWLDNLCLKSREGENSYVTLNECS---RDDKNQRWALYGDGTIR 215
           Q W    ++ P+V  I   + +CL++  G N  V+L +CS       +Q+WALYGDGTIR
Sbjct: 443 QGW-IVGNVEPLVTFIVGYEQMCLETNPGNND-VSLGDCSVKSASKVDQKWALYGDGTIR 502

Query: 216 QNEYRHLCLTSSERNYGALVVVSRCADKPQQRWGLAEDNTINHPNTNLVMDV-HREVPLL 275
            N  R LC+TS  ++    +++ +C     QRW    D TI++P++ LVM V   +VPL 
Sbjct: 503 VNNDRSLCVTSEGKSSNEPIIILKCLGWANQRWVFNTDGTISNPDSKLVMHVDQNDVPL- 562

Query: 276 PPMIVVDKHDGAPSQRW 283
              I++    G  +Q+W
Sbjct: 563 -RKIILSHPSGTSNQQW 562

BLAST of CmaCh04G005230 vs. Swiss-Prot
Match: ABRB_ABRPR (Abrin-b OS=Abrus precatorius PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.2e-29
Identity = 86/254 (33.86%), Postives = 135/254 (53.15%), Query Frame = 1

Query: 36  GRDGLCLDTMLLSGYYPQTDLS--QCQPR-KLSQLWTILEDGTIRASDVRYCLVPKTQFI 95
           GR+G+C+D +   GY+    +   +C+ R + +QLWT+  D TIR++    CL   T+  
Sbjct: 280 GRNGMCVD-VYDDGYHNGNRIIAWKCKDRLEENQLWTLKSDKTIRSNGK--CLT--TEGY 339

Query: 96  GPVGPNVVVMDCTKEVRHDMKWTKKKDGTIHHDESGLVLTAKPGRM---VTLEANEDAPS 155
            P G  V++ DCT  V     W    +GTI + +S LVL+A+   M   +T++ NE    
Sbjct: 340 AP-GNYVMIYDCTSAVAEATYWEIWDNGTIINPKSALVLSAESSSMGGTLTVQTNEYLMR 399

Query: 156 QSWEATESLYPMVANIKWLDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNE 215
           Q W    +  P V +I    +LC+   + + S V L  C  + K Q+WALY DG+IR  +
Sbjct: 400 QGWRTGNNTSPFVTSISGYSDLCM---QAQGSNVWLAYCDNNKKEQQWALYTDGSIRSVQ 459

Query: 216 YRHLCLTSSERNYGALVVVSRCADK-PQQRWGLAEDNTINHPNTNLVMDVHREVPLLPPM 275
             + CLTS +   G+ +V+  C++    QRW    D +I + + ++VMDV R  P L  +
Sbjct: 460 NTNNCLTSKDHKQGSPIVLMACSNGWASQRWLFRNDGSIYNLHDDMVMDVKRSDPSLKEI 519

Query: 276 IVVDKHDGAPSQRW 283
           I+   H G P+Q W
Sbjct: 520 ILHPYH-GKPNQIW 523

BLAST of CmaCh04G005230 vs. TrEMBL
Match: A0A0A0L756_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G219180 PE=4 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 2.4e-72
Identity = 157/296 (53.04%), Postives = 201/296 (67.91%), Query Frame = 1

Query: 4   MRQVLLCIALAFSL----AIAAPSEYG--AFGGRTHLVGRDGLCLDTM--LLSGYYPQTD 63
           M++++L I +AFSL    A+A PS+YG     G THLVGRDGLCL+       G    T 
Sbjct: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYGYQVVPGATHLVGRDGLCLEMSPWFYKGSNFPTR 60

Query: 64  LSQC-QPRKLSQLWTILEDGTIRASDVRYCLVP--KTQFIGPVGPNVVVMDCTKEVRHDM 123
           LS C + +K +QLWT+L+D TIR  + RYCLV      FI     NVVV +C KE   + 
Sbjct: 61  LSPCNEQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVNFI----TNVVVSECAKEPHSNK 120

Query: 124 KWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKWLDNLC 183
           KW  K+D TI H  SG+VLT      VT++ N++APSQSWEAT+SL PMVANIKWL+NLC
Sbjct: 121 KWIHKEDRTIVHVGSGMVLTGN-SNYVTVQRNKNAPSQSWEATKSLTPMVANIKWLENLC 180

Query: 184 LKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVVVSRCA 243
           L+S +  N YV L+ C+ ++K+Q W+LYGDGTIR++  R+ CLT SE+++G  V VS+C 
Sbjct: 181 LQSTKDSN-YVQLDGCNSENKSQHWSLYGDGTIRKHVNRNYCLT-SEQDFGRFVAVSKCE 240

Query: 244 DKPQQRWGL-AEDNTINHPNTNLVMDVHREVPL--LPPMIVVDKHDGAPSQRWSIY 286
           DKPQQRWGL AEDNTINHPNT++VMDV   VP   LP  +V +  DG  +QRW IY
Sbjct: 241 DKPQQRWGLGAEDNTINHPNTDMVMDV-LSVPFDDLPLAVVTNHRDGTATQRWIIY 288

BLAST of CmaCh04G005230 vs. TrEMBL
Match: A0A0A0LAC2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217140 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 2.6e-66
Identity = 148/298 (49.66%), Postives = 193/298 (64.77%), Query Frame = 1

Query: 4   MRQVLLCIALAFSLA----IAAPSEYG---AFGG---RTHLVGRDGLCLDTMLLSGYYP- 63
           M++++L I +AFSL     IA P  YG    +GG    THLVGRDGLCL+ +LL  Y P 
Sbjct: 1   MKEIVLSIIVAFSLTTQLVIAVPPNYGYGVGYGGVPGATHLVGRDGLCLEMVLLRSYKPA 60

Query: 64  ----QTDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKEV 123
                T L  C  +K +QLWT++ DGTIR  + R+CL     F G +    VV +C K  
Sbjct: 61  GINFPTRLLPCDEKKQTQLWTVVGDGTIRPMNDRFCLAADV-FYGVIN-KAVVSECGKVS 120

Query: 124 RHDMKWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKWL 183
             + +W  K DGTI   ES +VLT      VTL++ +  PSQSWE TESL PMVANI+WL
Sbjct: 121 DSNKQWIHKNDGTIALAESRMVLTG-DSDYVTLQSIKYTPSQSWEVTESLNPMVANIEWL 180

Query: 184 DNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVVV 243
           +NLCL+S + ++S V LN C+   K QRWALY DGTIRQ+  R+ CLT SE+NY   VVV
Sbjct: 181 NNLCLQSTD-DSSNVGLNGCNTYTKYQRWALYADGTIRQHVNRNYCLT-SEQNYDRSVVV 240

Query: 244 SRCADKPQQRWGL-AEDNTINHPNTNLVMDVHREVPLLPPMIVVDKH-DGAPSQRWSI 285
           S+C DKPQQRW L A+D+TI+HPNTN+V+DV   +    P+++V  H DG+ SQRW++
Sbjct: 241 SKCEDKPQQRWSLNAKDSTIDHPNTNMVLDVLNVLDSASPLVIVTNHPDGSASQRWTM 293

BLAST of CmaCh04G005230 vs. TrEMBL
Match: Q9SLV9_CUCSA (XSP30 OS=Cucumis sativus GN=XSP30 PE=2 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.9e-65
Identity = 148/300 (49.33%), Postives = 201/300 (67.00%), Query Frame = 1

Query: 4   MRQVLLCIALAFSL----AIAAPSEYG---AFGG---RTHLVGRDGLCLDTMLLSGYYPQ 63
           M++++L I +AFSL    AIA P  YG    +GG    THLVGRDGLCL+   +S +Y  
Sbjct: 1   MKEIVLSIIVAFSLTTQLAIAVPPNYGYGVGYGGVPGATHLVGRDGLCLE---MSPWYKP 60

Query: 64  ------TDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKE 123
                 T LS C  +K +QLWTI+ DGTIR  + ++CL  +  F G +    VV +C K 
Sbjct: 61  AGINFPTRLSPCDEKKQTQLWTIVGDGTIRPMNDKFCLAAEV-FYGVIN-KAVVSECGKV 120

Query: 124 VRHDMKWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKW 183
              + KWT+K DGTI   +S +VLT      VTL++N+  PSQSWE TESL  MVANI+W
Sbjct: 121 SDPNKKWTQKNDGTIALVDSRMVLTGDLD-YVTLQSNKYTPSQSWEVTESLNSMVANIEW 180

Query: 184 LDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVV 243
           L+NLCL+S + ++S+V LN C+ D+K QRWALY DGTIRQ+  ++ CLT S++++G  VV
Sbjct: 181 LNNLCLQSTD-DSSHVGLNGCNTDNKYQRWALYADGTIRQHVNKNYCLT-SDQDFGRFVV 240

Query: 244 VSRCADKPQQRWGL-AEDNTINHPNTNLVMDVHREVP--LLPPMIVVDKHDGAPSQRWSI 285
           VS+C DKPQQRW L A+D TI+HPNT++V+DV   VP    P ++V ++ DG+ SQRW+I
Sbjct: 241 VSKCEDKPQQRWSLDAKDYTIDHPNTDMVLDVF-SVPDSTFPSVLVTNRRDGSASQRWTI 291

BLAST of CmaCh04G005230 vs. TrEMBL
Match: A0A0A0L9D1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217160 PE=4 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 8.3e-65
Identity = 147/300 (49.00%), Postives = 200/300 (66.67%), Query Frame = 1

Query: 4   MRQVLLCIALAFSLA----IAAPSEYG---AFGG---RTHLVGRDGLCLDTMLLSGYYPQ 63
           M++++L I +AFSL     IA P  YG    +GG    THLVGRDGLCL+   +S +Y  
Sbjct: 1   MKEIVLSIIVAFSLTTQLVIAVPPNYGYGVGYGGVPGATHLVGRDGLCLE---MSPWYKP 60

Query: 64  ------TDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKE 123
                 T LS C  +K +QLWTI+ DGTIR  + ++CL  +  F G +    VV +C K 
Sbjct: 61  AGINFPTRLSPCDEKKQTQLWTIVGDGTIRPMNDKFCLAAEV-FYGVIN-KAVVSECGKV 120

Query: 124 VRHDMKWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKW 183
              + KWT+K DGTI   +S +VLT      VTL++N+  PSQSWE TESL  MVANI+W
Sbjct: 121 SDPNKKWTQKNDGTIALVDSRMVLTGDLD-YVTLQSNKYTPSQSWEVTESLNSMVANIEW 180

Query: 184 LDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVV 243
           L+NLCL+S + ++S+V LN C+ D+K QRWALY DGTIRQ+  ++ CLT S++++G  VV
Sbjct: 181 LNNLCLQSTD-DSSHVGLNGCNTDNKYQRWALYADGTIRQHVNKNYCLT-SDQDFGRFVV 240

Query: 244 VSRCADKPQQRWGL-AEDNTINHPNTNLVMDVHREVP--LLPPMIVVDKHDGAPSQRWSI 285
           VS+C DKPQQRW L A+D TI+HPNT++V+DV   VP    P ++V ++ DG+ SQRW+I
Sbjct: 241 VSKCEDKPQQRWSLDAKDYTIDHPNTDMVLDVF-SVPDSTFPSVLVTNRRDGSASQRWTI 291

BLAST of CmaCh04G005230 vs. TrEMBL
Match: Q58A89_SAMRA (Lectin OS=Sambucus racemosa subsp. sieboldiana GN=LecSSA-b-3a PE=2 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 9.9e-34
Identity = 91/255 (35.69%), Postives = 136/255 (53.33%), Query Frame = 1

Query: 33  HLVGRDGLCLDTMLLSGYYPQTDLSQCQP--RKLSQLWTILEDGTIRASDVRYCLVPKTQ 92
           +++GR+GLC+D    +GY       Q  P  R+ +Q WT  EDGTIR+  +  C+     
Sbjct: 21  NIIGRNGLCVDVR--NGYSTDGTPIQLWPCGRQRNQQWTFYEDGTIRS--MGKCMTANGL 80

Query: 93  FIGPVGPNVVVMDCTKEVRHDMKWTKKKDGTIHHDESGLVLTAKPGRMVT---LEANEDA 152
                G N+++ +C+  V + +KW    DG+I +  SGLV+TA      T   LE N  A
Sbjct: 81  ---NSGSNIMIFNCSTAVENAIKWEVTIDGSIINLSSGLVMTAPSAASRTILLLENNIYA 140

Query: 153 PSQSWEATESLYPMVANIKWLDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQ 212
            SQ W  +  + P+VA+I     +CL++  GEN+ V + +C      Q+WAL+GD TIR 
Sbjct: 141 ASQGWTVSNDVKPIVASIVGYKEMCLQAN-GENNGVWMEDCEVTSLQQQWALFGDRTIRV 200

Query: 213 NEYRHLCLTSSERNYGALVVVSRCADKPQQRWGLAEDNTINHPNTNLVMDVHREVPLLPP 272
           N  R LC+TS+  N   L++  +C   P QRW    +  I +PN+ LVMDV      L  
Sbjct: 201 NSSRGLCVTSNGYNSKDLIITLKCQGLPSQRWFFNSNGAIVNPNSTLVMDVRASNVSLRE 260

Query: 273 MIVVDKHDGAPSQRW 283
            I++    G P+Q+W
Sbjct: 261 -IIISPATGNPNQQW 266

BLAST of CmaCh04G005230 vs. NCBI nr
Match: gi|449457105|ref|XP_004146289.1| (PREDICTED: nigrin b-like [Cucumis sativus])

HSP 1 Score: 280.4 bits (716), Expect = 3.5e-72
Identity = 157/296 (53.04%), Postives = 201/296 (67.91%), Query Frame = 1

Query: 4   MRQVLLCIALAFSL----AIAAPSEYG--AFGGRTHLVGRDGLCLDTM--LLSGYYPQTD 63
           M++++L I +AFSL    A+A PS+YG     G THLVGRDGLCL+       G    T 
Sbjct: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYGYQVVPGATHLVGRDGLCLEMSPWFYKGSNFPTR 60

Query: 64  LSQC-QPRKLSQLWTILEDGTIRASDVRYCLVP--KTQFIGPVGPNVVVMDCTKEVRHDM 123
           LS C + +K +QLWT+L+D TIR  + RYCLV      FI     NVVV +C KE   + 
Sbjct: 61  LSPCNEQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVNFI----TNVVVSECAKEPHSNK 120

Query: 124 KWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKWLDNLC 183
           KW  K+D TI H  SG+VLT      VT++ N++APSQSWEAT+SL PMVANIKWL+NLC
Sbjct: 121 KWIHKEDRTIVHVGSGMVLTGN-SNYVTVQRNKNAPSQSWEATKSLTPMVANIKWLENLC 180

Query: 184 LKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVVVSRCA 243
           L+S +  N YV L+ C+ ++K+Q W+LYGDGTIR++  R+ CLT SE+++G  V VS+C 
Sbjct: 181 LQSTKDSN-YVQLDGCNSENKSQHWSLYGDGTIRKHVNRNYCLT-SEQDFGRFVAVSKCE 240

Query: 244 DKPQQRWGL-AEDNTINHPNTNLVMDVHREVPL--LPPMIVVDKHDGAPSQRWSIY 286
           DKPQQRWGL AEDNTINHPNT++VMDV   VP   LP  +V +  DG  +QRW IY
Sbjct: 241 DKPQQRWGLGAEDNTINHPNTDMVMDV-LSVPFDDLPLAVVTNHRDGTATQRWIIY 288

BLAST of CmaCh04G005230 vs. NCBI nr
Match: gi|659112133|ref|XP_008456079.1| (PREDICTED: nigrin b-like [Cucumis melo])

HSP 1 Score: 269.2 bits (687), Expect = 8.0e-69
Identity = 151/297 (50.84%), Postives = 202/297 (68.01%), Query Frame = 1

Query: 4   MRQVLLCIALAFSL----AIAAPSEYG---AFGG---RTHLVGRDGLCLDTM--LLSGYY 63
           M++++L I +AFSL    A+A PS YG    +GG    THLVGRDGLCL+       G  
Sbjct: 1   MKEIVLSIIVAFSLTTHLAMAVPSNYGYGGEYGGVPRATHLVGRDGLCLEMSPWFYRGNN 60

Query: 64  PQTDLSQC-QPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKEVRH 123
             T LS C + +K +QLWT++ DGTIR  + ++CLV     +G +    VV +C KE   
Sbjct: 61  FPTRLSPCDEHKKQTQLWTVVGDGTIRPMNDKFCLVADV-LVGLIN-KAVVRECAKEPNS 120

Query: 124 DMKWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKWLDN 183
           + +WT KKDGTI H ESG+VLT      VTL++N++ PSQSWEATES  PM+ANI+WL+N
Sbjct: 121 NKRWTHKKDGTITHVESGMVLTGDSD-YVTLQSNKNTPSQSWEATESSTPMIANIEWLNN 180

Query: 184 LCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVVVSR 243
           LCL+S +  N +V LN C+R++K+QRWALYGDGTIRQN  R+ CLTS E   G  +V+S+
Sbjct: 181 LCLQSTDDSN-HVGLNGCNRENKDQRWALYGDGTIRQNVNRNYCLTSDE-YLGRFIVMSK 240

Query: 244 CADKPQQRWGL-AEDNTINHPNTNLVMDVHREVPL--LPPMIVVDKHDGAPSQRWSI 285
           C DKPQQRW L A+D+TI+HPN+++VMDV   VP       +V ++ DG+ SQRW+I
Sbjct: 241 CEDKPQQRWNLGAKDSTIDHPNSDMVMDV-LFVPYYDFSSAVVTNRRDGSASQRWTI 291

BLAST of CmaCh04G005230 vs. NCBI nr
Match: gi|778680239|ref|XP_004151509.2| (PREDICTED: nigrin b-like [Cucumis sativus])

HSP 1 Score: 260.4 bits (664), Expect = 3.7e-66
Identity = 148/298 (49.66%), Postives = 193/298 (64.77%), Query Frame = 1

Query: 4   MRQVLLCIALAFSLA----IAAPSEYG---AFGG---RTHLVGRDGLCLDTMLLSGYYP- 63
           M++++L I +AFSL     IA P  YG    +GG    THLVGRDGLCL+ +LL  Y P 
Sbjct: 1   MKEIVLSIIVAFSLTTQLVIAVPPNYGYGVGYGGVPGATHLVGRDGLCLEMVLLRSYKPA 60

Query: 64  ----QTDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKEV 123
                T L  C  +K +QLWT++ DGTIR  + R+CL     F G +    VV +C K  
Sbjct: 61  GINFPTRLLPCDEKKQTQLWTVVGDGTIRPMNDRFCLAADV-FYGVIN-KAVVSECGKVS 120

Query: 124 RHDMKWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKWL 183
             + +W  K DGTI   ES +VLT      VTL++ +  PSQSWE TESL PMVANI+WL
Sbjct: 121 DSNKQWIHKNDGTIALAESRMVLTG-DSDYVTLQSIKYTPSQSWEVTESLNPMVANIEWL 180

Query: 184 DNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVVV 243
           +NLCL+S + ++S V LN C+   K QRWALY DGTIRQ+  R+ CLT SE+NY   VVV
Sbjct: 181 NNLCLQSTD-DSSNVGLNGCNTYTKYQRWALYADGTIRQHVNRNYCLT-SEQNYDRSVVV 240

Query: 244 SRCADKPQQRWGL-AEDNTINHPNTNLVMDVHREVPLLPPMIVVDKH-DGAPSQRWSI 285
           S+C DKPQQRW L A+D+TI+HPNTN+V+DV   +    P+++V  H DG+ SQRW++
Sbjct: 241 SKCEDKPQQRWSLNAKDSTIDHPNTNMVLDVLNVLDSASPLVIVTNHPDGSASQRWTM 293

BLAST of CmaCh04G005230 vs. NCBI nr
Match: gi|566006087|ref|NP_001274403.1| (uncharacterized protein LOC101221335 precursor [Cucumis sativus])

HSP 1 Score: 256.9 bits (655), Expect = 4.1e-65
Identity = 148/300 (49.33%), Postives = 201/300 (67.00%), Query Frame = 1

Query: 4   MRQVLLCIALAFSL----AIAAPSEYG---AFGG---RTHLVGRDGLCLDTMLLSGYYPQ 63
           M++++L I +AFSL    AIA P  YG    +GG    THLVGRDGLCL+   +S +Y  
Sbjct: 1   MKEIVLSIIVAFSLTTQLAIAVPPNYGYGVGYGGVPGATHLVGRDGLCLE---MSPWYKP 60

Query: 64  ------TDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKE 123
                 T LS C  +K +QLWTI+ DGTIR  + ++CL  +  F G +    VV +C K 
Sbjct: 61  AGINFPTRLSPCDEKKQTQLWTIVGDGTIRPMNDKFCLAAEV-FYGVIN-KAVVSECGKV 120

Query: 124 VRHDMKWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKW 183
              + KWT+K DGTI   +S +VLT      VTL++N+  PSQSWE TESL  MVANI+W
Sbjct: 121 SDPNKKWTQKNDGTIALVDSRMVLTGDLD-YVTLQSNKYTPSQSWEVTESLNSMVANIEW 180

Query: 184 LDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVV 243
           L+NLCL+S + ++S+V LN C+ D+K QRWALY DGTIRQ+  ++ CLT S++++G  VV
Sbjct: 181 LNNLCLQSTD-DSSHVGLNGCNTDNKYQRWALYADGTIRQHVNKNYCLT-SDQDFGRFVV 240

Query: 244 VSRCADKPQQRWGL-AEDNTINHPNTNLVMDVHREVP--LLPPMIVVDKHDGAPSQRWSI 285
           VS+C DKPQQRW L A+D TI+HPNT++V+DV   VP    P ++V ++ DG+ SQRW+I
Sbjct: 241 VSKCEDKPQQRWSLDAKDYTIDHPNTDMVLDVF-SVPDSTFPSVLVTNRRDGSASQRWTI 291

BLAST of CmaCh04G005230 vs. NCBI nr
Match: gi|700202446|gb|KGN57579.1| (hypothetical protein Csa_3G217160 [Cucumis sativus])

HSP 1 Score: 255.4 bits (651), Expect = 1.2e-64
Identity = 147/300 (49.00%), Postives = 200/300 (66.67%), Query Frame = 1

Query: 4   MRQVLLCIALAFSLA----IAAPSEYG---AFGG---RTHLVGRDGLCLDTMLLSGYYPQ 63
           M++++L I +AFSL     IA P  YG    +GG    THLVGRDGLCL+   +S +Y  
Sbjct: 1   MKEIVLSIIVAFSLTTQLVIAVPPNYGYGVGYGGVPGATHLVGRDGLCLE---MSPWYKP 60

Query: 64  ------TDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKE 123
                 T LS C  +K +QLWTI+ DGTIR  + ++CL  +  F G +    VV +C K 
Sbjct: 61  AGINFPTRLSPCDEKKQTQLWTIVGDGTIRPMNDKFCLAAEV-FYGVIN-KAVVSECGKV 120

Query: 124 VRHDMKWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKW 183
              + KWT+K DGTI   +S +VLT      VTL++N+  PSQSWE TESL  MVANI+W
Sbjct: 121 SDPNKKWTQKNDGTIALVDSRMVLTGDLD-YVTLQSNKYTPSQSWEVTESLNSMVANIEW 180

Query: 184 LDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVV 243
           L+NLCL+S + ++S+V LN C+ D+K QRWALY DGTIRQ+  ++ CLT S++++G  VV
Sbjct: 181 LNNLCLQSTD-DSSHVGLNGCNTDNKYQRWALYADGTIRQHVNKNYCLT-SDQDFGRFVV 240

Query: 244 VSRCADKPQQRWGL-AEDNTINHPNTNLVMDVHREVP--LLPPMIVVDKHDGAPSQRWSI 285
           VS+C DKPQQRW L A+D TI+HPNT++V+DV   VP    P ++V ++ DG+ SQRW+I
Sbjct: 241 VSKCEDKPQQRWSLDAKDYTIDHPNTDMVLDVF-SVPDSTFPSVLVTNRRDGSASQRWTI 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NIGB_SAMNI2.2e-3435.69Nigrin b OS=Sambucus nigra PE=1 SV=2[more]
ABRC_ABRPR1.1e-3034.25Abrin-c OS=Abrus precatorius PE=1 SV=1[more]
SGSL_TRIAN4.3e-3032.44Seed lectin (Fragments) OS=Trichosanthes anguina PE=1 SV=2[more]
RIP1_SAMNI7.3e-3034.24Ribosome-inactivating protein SNAI' OS=Sambucus nigra PE=1 SV=1[more]
ABRB_ABRPR1.2e-2933.86Abrin-b OS=Abrus precatorius PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L756_CUCSA2.4e-7253.04Uncharacterized protein OS=Cucumis sativus GN=Csa_3G219180 PE=4 SV=1[more]
A0A0A0LAC2_CUCSA2.6e-6649.66Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217140 PE=4 SV=1[more]
Q9SLV9_CUCSA2.9e-6549.33XSP30 OS=Cucumis sativus GN=XSP30 PE=2 SV=1[more]
A0A0A0L9D1_CUCSA8.3e-6549.00Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217160 PE=4 SV=1[more]
Q58A89_SAMRA9.9e-3435.69Lectin OS=Sambucus racemosa subsp. sieboldiana GN=LecSSA-b-3a PE=2 SV=1[more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
gi|449457105|ref|XP_004146289.1|3.5e-7253.04PREDICTED: nigrin b-like [Cucumis sativus][more]
gi|659112133|ref|XP_008456079.1|8.0e-6950.84PREDICTED: nigrin b-like [Cucumis melo][more]
gi|778680239|ref|XP_004151509.2|3.7e-6649.66PREDICTED: nigrin b-like [Cucumis sativus][more]
gi|566006087|ref|NP_001274403.1|4.1e-6549.33uncharacterized protein LOC101221335 precursor [Cucumis sativus][more]
gi|700202446|gb|KGN57579.1|1.2e-6449.00hypothetical protein Csa_3G217160 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000772Ricin_B_lectin
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G005230.1CmaCh04G005230.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000772Ricin B, lectin domainPFAMPF00652Ricin_B_lectincoord: 169..282
score: 5.6E-22coord: 33..152
score: 1.5
IPR000772Ricin B, lectin domainSMARTSM00458ricin_3coord: 29..155
score: 5.3E-4coord: 159..285
score: 9.6
IPR000772Ricin B, lectin domainPROFILEPS50231RICIN_B_LECTINcoord: 31..155
score: 21.382coord: 169..285
score: 27
IPR000772Ricin B, lectin domainunknownSSF50370Ricin B-like lectinscoord: 32..153
score: 1.91E-16coord: 159..284
score: 1.2
NoneNo IPR availableGENE3DG3DSA:2.80.10.50coord: 33..153
score: 2.8E-15coord: 164..284
score: 5.0

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh04G005230Lsi05G001810Bottle gourd (USVL1VR-Ls)cmalsiB690
The following gene(s) are paralogous to this gene:

None