CmaCh04G005230 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh04G005230
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	rRNA N-glycosidase
Location	Cma_Chr04 : 2644555 .. 2645412 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAAGATGAGACAAGTCCTGCTCTGCATTGCCCTGGCATTCTCCCTCGCCATAGCGGCGCCCTCTGAGTACGGAGCGTTCGGAGGACGCACACATCTTGTTGGTCGAGATGGGCTGTGTTTAGACACCATGTTGCTTTCCGGATATTATCCTCAAACTGACTTATCCCAATGCCAACCAAGGAAACTGAGCCAGCTATGGACCATCCTTGAAGATGGCACGATTCGAGCCAGTGATGTTAGATACTGTTTGGTTCCTAAAACTCAATTCATTGGACCCGTTGGACCCAATGTCGTAGTAATGGACTGCACAAAAGAGGTAAGACACGACATGAAATGGACCAAAAAGAAGGATGGAACCATACACCACGATGAGTCGGGGCTTGTCCTGACGGCGAAACCAGGCAGGATGGTGACATTGGAAGCCAATGAAGACGCGCCATCACAAAGCTGGGAAGCCACGGAGAGCCTCTACCCAATGGTGGCTAATATCAAATGGCTTGATAACTTGTGTTTGAAATCTAGAGAAGGTGAAAATTCGTATGTGACGTTGAACGAGTGTAGCCGAGATGATAAGAACCAACGATGGGCGTTGTATGGGGACGGCACCATTCGACAAAATGAGTATAGACACTTGTGCTTGACTTCGTCCGAAAGGAATTATGGTGCGCTGGTGGTTGTGTCTAGGTGCGCTGACAAGCCACAGCAGCGTTGGGGGCTTGCGGAAGACAACACCATCAACCATCCCAACACCAACTTGGTCATGGATGTGCATAGGGAGGTGCCTCTGTTGCCGCCCATGATCGTGGTTGACAAGCATGACGGCGCTCCTAGCCAACGATGGTCCATTTACTGA

mRNA sequence

ATGGAGAAGATGAGACAAGTCCTGCTCTGCATTGCCCTGGCATTCTCCCTCGCCATAGCGGCGCCCTCTGAGTACGGAGCGTTCGGAGGACGCACACATCTTGTTGGTCGAGATGGGCTGTGTTTAGACACCATGTTGCTTTCCGGATATTATCCTCAAACTGACTTATCCCAATGCCAACCAAGGAAACTGAGCCAGCTATGGACCATCCTTGAAGATGGCACGATTCGAGCCAGTGATGTTAGATACTGTTTGGTTCCTAAAACTCAATTCATTGGACCCGTTGGACCCAATGTCGTAGTAATGGACTGCACAAAAGAGGTAAGACACGACATGAAATGGACCAAAAAGAAGGATGGAACCATACACCACGATGAGTCGGGGCTTGTCCTGACGGCGAAACCAGGCAGGATGGTGACATTGGAAGCCAATGAAGACGCGCCATCACAAAGCTGGGAAGCCACGGAGAGCCTCTACCCAATGGTGGCTAATATCAAATGGCTTGATAACTTGTGTTTGAAATCTAGAGAAGGTGAAAATTCGTATGTGACGTTGAACGAGTGTAGCCGAGATGATAAGAACCAACGATGGGCGTTGTATGGGGACGGCACCATTCGACAAAATGAGTATAGACACTTGTGCTTGACTTCGTCCGAAAGGAATTATGGTGCGCTGGTGGTTGTGTCTAGGTGCGCTGACAAGCCACAGCAGCGTTGGGGGCTTGCGGAAGACAACACCATCAACCATCCCAACACCAACTTGGTCATGGATGTGCATAGGGAGGTGCCTCTGTTGCCGCCCATGATCGTGGTTGACAAGCATGACGGCGCTCCTAGCCAACGATGGTCCATTTACTGA

Coding sequence (CDS)

ATGGAGAAGATGAGACAAGTCCTGCTCTGCATTGCCCTGGCATTCTCCCTCGCCATAGCGGCGCCCTCTGAGTACGGAGCGTTCGGAGGACGCACACATCTTGTTGGTCGAGATGGGCTGTGTTTAGACACCATGTTGCTTTCCGGATATTATCCTCAAACTGACTTATCCCAATGCCAACCAAGGAAACTGAGCCAGCTATGGACCATCCTTGAAGATGGCACGATTCGAGCCAGTGATGTTAGATACTGTTTGGTTCCTAAAACTCAATTCATTGGACCCGTTGGACCCAATGTCGTAGTAATGGACTGCACAAAAGAGGTAAGACACGACATGAAATGGACCAAAAAGAAGGATGGAACCATACACCACGATGAGTCGGGGCTTGTCCTGACGGCGAAACCAGGCAGGATGGTGACATTGGAAGCCAATGAAGACGCGCCATCACAAAGCTGGGAAGCCACGGAGAGCCTCTACCCAATGGTGGCTAATATCAAATGGCTTGATAACTTGTGTTTGAAATCTAGAGAAGGTGAAAATTCGTATGTGACGTTGAACGAGTGTAGCCGAGATGATAAGAACCAACGATGGGCGTTGTATGGGGACGGCACCATTCGACAAAATGAGTATAGACACTTGTGCTTGACTTCGTCCGAAAGGAATTATGGTGCGCTGGTGGTTGTGTCTAGGTGCGCTGACAAGCCACAGCAGCGTTGGGGGCTTGCGGAAGACAACACCATCAACCATCCCAACACCAACTTGGTCATGGATGTGCATAGGGAGGTGCCTCTGTTGCCGCCCATGATCGTGGTTGACAAGCATGACGGCGCTCCTAGCCAACGATGGTCCATTTACTGA

Protein sequence

MEKMRQVLLCIALAFSLAIAAPSEYGAFGGRTHLVGRDGLCLDTMLLSGYYPQTDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKEVRHDMKWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKWLDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVVVSRCADKPQQRWGLAEDNTINHPNTNLVMDVHREVPLLPPMIVVDKHDGAPSQRWSIY

Homology

[Close]

BLAST of CmaCh04G005230 vs. Swiss-Prot
Match: NIGB_SAMNI (Nigrin b OS=Sambucus nigra PE=1 SV=2)

HSP 1 Score: 147.5 bits (371), Expect = 2.2e-34
Identity = 91/255 (35.69%), Postives = 132/255 (51.76%), Query Frame = 1


Query: 33  HLVGRDGLCLDTMLLSGYYPQTDLSQCQP--RKLSQLWTILEDGTIRASDVRYCLVPKTQ 92
           ++VGRDGLC+D    +GY       Q  P   + +Q WT   D TIR+  +  C+     
Sbjct: 311 NIVGRDGLCVDVR--NGYDTDGTPLQLWPCGTQRNQRWTFDSDDTIRS--MGKCMTANGL 370

Query: 93  FIGPVGPNVVVMDCTKEVRHDMKWTKKKDGTIHHDESGLVLTAKPGRMVT---LEANEDA 152
                G N+V+ +C+    + +KW    DG+I +  SGLV+TA      T   LE N  A
Sbjct: 371 ---NNGSNIVIFNCSTAAENAIKWEVPIDGSIINPSSGLVMTAPRAASRTILLLEDNIYA 430

Query: 153 PSQSWEATESLYPMVANIKWLDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQ 212
            SQ W  T ++ P+VA+I     +CL+S  GEN+ V + +C      Q+WALYGD TIR 
Sbjct: 431 ASQGWTVTNNVKPIVASIVGYKEMCLQSN-GENNGVWMEDCEATSLQQQWALYGDRTIRV 490

Query: 213 NEYRHLCLTSSERNYGALVVVSRCADKPQQRWGLAEDNTINHPNTNLVMDVHREVPLLPP 272
           N  R LC+T++  N   L+++ +C   P QRW    D  I +P +  VMDV      L  
Sbjct: 491 NSTRGLCVTTNGYNSKDLIIILKCQGLPSQRWFFNSDGAIVNPKSRHVMDVRASNVSLRE 550

Query: 273 MIVVDKHDGAPSQRW 283
           +I+     G P+Q+W
Sbjct: 551 IIIFPA-TGNPNQQW 556

[Close]

BLAST of CmaCh04G005230 vs. Swiss-Prot
Match: ABRC_ABRPR (Abrin-c OS=Abrus precatorius PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.1e-30
Identity = 87/254 (34.25%), Postives = 136/254 (53.54%), Query Frame = 1


Query: 36  GRDGLCLDTMLLSGYYPQTDLS--QCQPR-KLSQLWTILEDGTIRASDVRYCLVPKTQFI 95
           GRDG+C+D +   GY+    +   +C+ R + +QLWT+  D TIR++    CL   T+  
Sbjct: 315 GRDGMCVD-VYDDGYHNGNRIIAWKCKDRLEENQLWTLKSDKTIRSNGK--CLT--TEGY 374

Query: 96  GPVGPNVVVMDCTKEVRHDMKWTKKKDGTIHHDESGLVLTAKPGRM---VTLEANEDAPS 155
            P G  V++ DCT  V     W    +GTI + +S LVL+A+   M   +T++ NE    
Sbjct: 375 AP-GNYVMIYDCTSAVAEATYWEIWDNGTIINPKSALVLSAESSSMGGTLTVQTNEYLMR 434

Query: 156 QSWEATESLYPMVANIKWLDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNE 215
           Q W    +  P V +I    +LC+   + + S V L +C  + K Q+WALY DG+IR  +
Sbjct: 435 QGWRTGNNTSPFVTSISGYSDLCM---QAQGSNVWLADCDNNKKEQQWALYTDGSIRSVQ 494

Query: 216 YRHLCLTSSERNYGALVVVSRCADK-PQQRWGLAEDNTINHPNTNLVMDVHREVPLLPPM 275
             + CLTS +   G+ +V+  C++    QRW    D +I + + ++VMDV R  P L  +
Sbjct: 495 NTNNCLTSKDHKQGSPIVLMACSNGWASQRWLFKNDGSIYNLHDDMVMDVKRSDPSLKEI 554

Query: 276 IVVDKHDGAPSQRW 283
           I+   H G P+Q W
Sbjct: 555 ILHPYH-GKPNQIW 558

[Close]

BLAST of CmaCh04G005230 vs. Swiss-Prot
Match: SGSL_TRIAN (Seed lectin (Fragments) OS=Trichosanthes anguina PE=1 SV=2)

HSP 1 Score: 133.3 bits (334), Expect = 4.3e-30
Identity = 85/262 (32.44%), Postives = 133/262 (50.76%), Query Frame = 1


Query: 32  THLVGRDGLCLDTM-LLSGYYPQTDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQ 91
           T + GRD LC+D    L+    +  L  C  ++++Q WT   DGT+R+  +  CL     
Sbjct: 265 TRISGRDALCVDVAGALTSDGSRLILYPCG-QQVNQKWTFHSDGTVRS--LGKCLATNNS 324

Query: 92  FIGPVGPNVVVMDCTKEVRHDMKWTKKKDGTIHHDE-SGLVLTAKPGRM---VTLEANED 151
             G +   VV+ DC+K    D+ W     GTI +     L LT+        +T+E N  
Sbjct: 325 KFGNL---VVIYDCSKLAAEDISWDVSVGGTIMNPNYEDLALTSNKATRSTNLTMEVNTY 384

Query: 152 APSQSWEATESLYPMVANIKWLDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIR 211
           + SQ W     + P++ +I  LD++CL++ +G N+ + L EC  + + Q WALY DGTIR
Sbjct: 385 SASQGWRVGNYVQPIIGSIVGLDDMCLEATDG-NTNMWLEECVPNQREQSWALYSDGTIR 444

Query: 212 QNEYRHLCLTSSERNYG--ALVVVSRCADKPQQRWGLAEDNTINHP-NTNLVMDVHREVP 271
            ++ R LC+T+S   Y    ++ +  C     QRW    D +I+ P N  L MDV R   
Sbjct: 445 VDDNRELCVTASSSTYDNWKVITILNCDGSNNQRWVFLADGSISTPGNQRLAMDVARSDV 504

Query: 272 LLPPMIVVDKHDGAPSQRWSIY 286
            L  +I+   H G  +Q+W ++
Sbjct: 505 DLKKIILHRPH-GDLNQQWVLF 518

[Close]

BLAST of CmaCh04G005230 vs. Swiss-Prot
Match: RIP1_SAMNI (Ribosome-inactivating protein SNAI' OS=Sambucus nigra PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 7.3e-30
Identity = 88/257 (34.24%), Postives = 130/257 (50.58%), Query Frame = 1


Query: 36  GRDGLCLDTMLLSGYYPQ---TDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFI 95
           GRDG C +    +G         LS C  +  +Q WT   DGTI++  +  CL   +   
Sbjct: 323 GRDGFCAEVK--NGDEKDGTPVQLSSCGEQS-NQQWTFSTDGTIQS--LGKCLTTSS--- 382

Query: 96  GPVGPNVVVMDCTKEVRHDMKWTKKKDGTIHHDESGLVLTAKP---GRMVTLEANEDAPS 155
                +V++ +C        KW    DGTI +  SGLVLTA     G +V+LE N  A  
Sbjct: 383 -----SVMIYNCKVVPPESTKWVVSIDGTITNPRSGLVLTAPKAAEGTLVSLEKNVHAAR 442

Query: 156 QSWEATESLYPMVANIKWLDNLCLKSREGENSYVTLNECS---RDDKNQRWALYGDGTIR 215
           Q W    ++ P+V  I   + +CL++  G N  V+L +CS       +Q+WALYGDGTIR
Sbjct: 443 QGW-IVGNVEPLVTFIVGYEQMCLETNPGNND-VSLGDCSVKSASKVDQKWALYGDGTIR 502

Query: 216 QNEYRHLCLTSSERNYGALVVVSRCADKPQQRWGLAEDNTINHPNTNLVMDV-HREVPLL 275
            N  R LC+TS  ++    +++ +C     QRW    D TI++P++ LVM V   +VPL 
Sbjct: 503 VNNDRSLCVTSEGKSSNEPIIILKCLGWANQRWVFNTDGTISNPDSKLVMHVDQNDVPL- 562

Query: 276 PPMIVVDKHDGAPSQRW 283
              I++    G  +Q+W
Sbjct: 563 -RKIILSHPSGTSNQQW 562

[Close]

BLAST of CmaCh04G005230 vs. Swiss-Prot
Match: ABRB_ABRPR (Abrin-b OS=Abrus precatorius PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.2e-29
Identity = 86/254 (33.86%), Postives = 135/254 (53.15%), Query Frame = 1


Query: 36  GRDGLCLDTMLLSGYYPQTDLS--QCQPR-KLSQLWTILEDGTIRASDVRYCLVPKTQFI 95
           GR+G+C+D +   GY+    +   +C+ R + +QLWT+  D TIR++    CL   T+  
Sbjct: 280 GRNGMCVD-VYDDGYHNGNRIIAWKCKDRLEENQLWTLKSDKTIRSNGK--CLT--TEGY 339

Query: 96  GPVGPNVVVMDCTKEVRHDMKWTKKKDGTIHHDESGLVLTAKPGRM---VTLEANEDAPS 155
            P G  V++ DCT  V     W    +GTI + +S LVL+A+   M   +T++ NE    
Sbjct: 340 AP-GNYVMIYDCTSAVAEATYWEIWDNGTIINPKSALVLSAESSSMGGTLTVQTNEYLMR 399

Query: 156 QSWEATESLYPMVANIKWLDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNE 215
           Q W    +  P V +I    +LC+   + + S V L  C  + K Q+WALY DG+IR  +
Sbjct: 400 QGWRTGNNTSPFVTSISGYSDLCM---QAQGSNVWLAYCDNNKKEQQWALYTDGSIRSVQ 459

Query: 216 YRHLCLTSSERNYGALVVVSRCADK-PQQRWGLAEDNTINHPNTNLVMDVHREVPLLPPM 275
             + CLTS +   G+ +V+  C++    QRW    D +I + + ++VMDV R  P L  +
Sbjct: 460 NTNNCLTSKDHKQGSPIVLMACSNGWASQRWLFRNDGSIYNLHDDMVMDVKRSDPSLKEI 519

Query: 276 IVVDKHDGAPSQRW 283
           I+   H G P+Q W
Sbjct: 520 ILHPYH-GKPNQIW 523

[Close]

BLAST of CmaCh04G005230 vs. TrEMBL
Match: A0A0A0L756_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G219180 PE=4 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 2.4e-72
Identity = 157/296 (53.04%), Postives = 201/296 (67.91%), Query Frame = 1


Query: 4   MRQVLLCIALAFSL----AIAAPSEYG--AFGGRTHLVGRDGLCLDTM--LLSGYYPQTD 63
           M++++L I +AFSL    A+A PS+YG     G THLVGRDGLCL+       G    T 
Sbjct: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYGYQVVPGATHLVGRDGLCLEMSPWFYKGSNFPTR 60

Query: 64  LSQC-QPRKLSQLWTILEDGTIRASDVRYCLVP--KTQFIGPVGPNVVVMDCTKEVRHDM 123
           LS C + +K +QLWT+L+D TIR  + RYCLV      FI     NVVV +C KE   + 
Sbjct: 61  LSPCNEQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVNFI----TNVVVSECAKEPHSNK 120

Query: 124 KWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKWLDNLC 183
           KW  K+D TI H  SG+VLT      VT++ N++APSQSWEAT+SL PMVANIKWL+NLC
Sbjct: 121 KWIHKEDRTIVHVGSGMVLTGN-SNYVTVQRNKNAPSQSWEATKSLTPMVANIKWLENLC 180

Query: 184 LKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVVVSRCA 243
           L+S +  N YV L+ C+ ++K+Q W+LYGDGTIR++  R+ CLT SE+++G  V VS+C 
Sbjct: 181 LQSTKDSN-YVQLDGCNSENKSQHWSLYGDGTIRKHVNRNYCLT-SEQDFGRFVAVSKCE 240

Query: 244 DKPQQRWGL-AEDNTINHPNTNLVMDVHREVPL--LPPMIVVDKHDGAPSQRWSIY 286
           DKPQQRWGL AEDNTINHPNT++VMDV   VP   LP  +V +  DG  +QRW IY
Sbjct: 241 DKPQQRWGLGAEDNTINHPNTDMVMDV-LSVPFDDLPLAVVTNHRDGTATQRWIIY 288

[Close]

BLAST of CmaCh04G005230 vs. TrEMBL
Match: A0A0A0LAC2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217140 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 2.6e-66
Identity = 148/298 (49.66%), Postives = 193/298 (64.77%), Query Frame = 1


Query: 4   MRQVLLCIALAFSLA----IAAPSEYG---AFGG---RTHLVGRDGLCLDTMLLSGYYP- 63
           M++++L I +AFSL     IA P  YG    +GG    THLVGRDGLCL+ +LL  Y P 
Sbjct: 1   MKEIVLSIIVAFSLTTQLVIAVPPNYGYGVGYGGVPGATHLVGRDGLCLEMVLLRSYKPA 60

Query: 64  ----QTDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKEV 123
                T L  C  +K +QLWT++ DGTIR  + R+CL     F G +    VV +C K  
Sbjct: 61  GINFPTRLLPCDEKKQTQLWTVVGDGTIRPMNDRFCLAADV-FYGVIN-KAVVSECGKVS 120

Query: 124 RHDMKWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKWL 183
             + +W  K DGTI   ES +VLT      VTL++ +  PSQSWE TESL PMVANI+WL
Sbjct: 121 DSNKQWIHKNDGTIALAESRMVLTG-DSDYVTLQSIKYTPSQSWEVTESLNPMVANIEWL 180

Query: 184 DNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVVV 243
           +NLCL+S + ++S V LN C+   K QRWALY DGTIRQ+  R+ CLT SE+NY   VVV
Sbjct: 181 NNLCLQSTD-DSSNVGLNGCNTYTKYQRWALYADGTIRQHVNRNYCLT-SEQNYDRSVVV 240

Query: 244 SRCADKPQQRWGL-AEDNTINHPNTNLVMDVHREVPLLPPMIVVDKH-DGAPSQRWSI 285
           S+C DKPQQRW L A+D+TI+HPNTN+V+DV   +    P+++V  H DG+ SQRW++
Sbjct: 241 SKCEDKPQQRWSLNAKDSTIDHPNTNMVLDVLNVLDSASPLVIVTNHPDGSASQRWTM 293

[Close]

BLAST of CmaCh04G005230 vs. TrEMBL
Match: Q9SLV9_CUCSA (XSP30 OS=Cucumis sativus GN=XSP30 PE=2 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.9e-65
Identity = 148/300 (49.33%), Postives = 201/300 (67.00%), Query Frame = 1


Query: 4   MRQVLLCIALAFSL----AIAAPSEYG---AFGG---RTHLVGRDGLCLDTMLLSGYYPQ 63
           M++++L I +AFSL    AIA P  YG    +GG    THLVGRDGLCL+   +S +Y  
Sbjct: 1   MKEIVLSIIVAFSLTTQLAIAVPPNYGYGVGYGGVPGATHLVGRDGLCLE---MSPWYKP 60

Query: 64  ------TDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKE 123
                 T LS C  +K +QLWTI+ DGTIR  + ++CL  +  F G +    VV +C K 
Sbjct: 61  AGINFPTRLSPCDEKKQTQLWTIVGDGTIRPMNDKFCLAAEV-FYGVIN-KAVVSECGKV 120

Query: 124 VRHDMKWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKW 183
              + KWT+K DGTI   +S +VLT      VTL++N+  PSQSWE TESL  MVANI+W
Sbjct: 121 SDPNKKWTQKNDGTIALVDSRMVLTGDLD-YVTLQSNKYTPSQSWEVTESLNSMVANIEW 180

Query: 184 LDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVV 243
           L+NLCL+S + ++S+V LN C+ D+K QRWALY DGTIRQ+  ++ CLT S++++G  VV
Sbjct: 181 LNNLCLQSTD-DSSHVGLNGCNTDNKYQRWALYADGTIRQHVNKNYCLT-SDQDFGRFVV 240

Query: 244 VSRCADKPQQRWGL-AEDNTINHPNTNLVMDVHREVP--LLPPMIVVDKHDGAPSQRWSI 285
           VS+C DKPQQRW L A+D TI+HPNT++V+DV   VP    P ++V ++ DG+ SQRW+I
Sbjct: 241 VSKCEDKPQQRWSLDAKDYTIDHPNTDMVLDVF-SVPDSTFPSVLVTNRRDGSASQRWTI 291

[Close]

BLAST of CmaCh04G005230 vs. TrEMBL
Match: A0A0A0L9D1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217160 PE=4 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 8.3e-65
Identity = 147/300 (49.00%), Postives = 200/300 (66.67%), Query Frame = 1


Query: 4   MRQVLLCIALAFSLA----IAAPSEYG---AFGG---RTHLVGRDGLCLDTMLLSGYYPQ 63
           M++++L I +AFSL     IA P  YG    +GG    THLVGRDGLCL+   +S +Y  
Sbjct: 1   MKEIVLSIIVAFSLTTQLVIAVPPNYGYGVGYGGVPGATHLVGRDGLCLE---MSPWYKP 60

Query: 64  ------TDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKE 123
                 T LS C  +K +QLWTI+ DGTIR  + ++CL  +  F G +    VV +C K 
Sbjct: 61  AGINFPTRLSPCDEKKQTQLWTIVGDGTIRPMNDKFCLAAEV-FYGVIN-KAVVSECGKV 120

Query: 124 VRHDMKWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKW 183
              + KWT+K DGTI   +S +VLT      VTL++N+  PSQSWE TESL  MVANI+W
Sbjct: 121 SDPNKKWTQKNDGTIALVDSRMVLTGDLD-YVTLQSNKYTPSQSWEVTESLNSMVANIEW 180

Query: 184 LDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVV 243
           L+NLCL+S + ++S+V LN C+ D+K QRWALY DGTIRQ+  ++ CLT S++++G  VV
Sbjct: 181 LNNLCLQSTD-DSSHVGLNGCNTDNKYQRWALYADGTIRQHVNKNYCLT-SDQDFGRFVV 240

Query: 244 VSRCADKPQQRWGL-AEDNTINHPNTNLVMDVHREVP--LLPPMIVVDKHDGAPSQRWSI 285
           VS+C DKPQQRW L A+D TI+HPNT++V+DV   VP    P ++V ++ DG+ SQRW+I
Sbjct: 241 VSKCEDKPQQRWSLDAKDYTIDHPNTDMVLDVF-SVPDSTFPSVLVTNRRDGSASQRWTI 291

[Close]

BLAST of CmaCh04G005230 vs. TrEMBL
Match: Q58A89_SAMRA (Lectin OS=Sambucus racemosa subsp. sieboldiana GN=LecSSA-b-3a PE=2 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 9.9e-34
Identity = 91/255 (35.69%), Postives = 136/255 (53.33%), Query Frame = 1


Query: 33  HLVGRDGLCLDTMLLSGYYPQTDLSQCQP--RKLSQLWTILEDGTIRASDVRYCLVPKTQ 92
           +++GR+GLC+D    +GY       Q  P  R+ +Q WT  EDGTIR+  +  C+     
Sbjct: 21  NIIGRNGLCVDVR--NGYSTDGTPIQLWPCGRQRNQQWTFYEDGTIRS--MGKCMTANGL 80

Query: 93  FIGPVGPNVVVMDCTKEVRHDMKWTKKKDGTIHHDESGLVLTAKPGRMVT---LEANEDA 152
                G N+++ +C+  V + +KW    DG+I +  SGLV+TA      T   LE N  A
Sbjct: 81  ---NSGSNIMIFNCSTAVENAIKWEVTIDGSIINLSSGLVMTAPSAASRTILLLENNIYA 140

Query: 153 PSQSWEATESLYPMVANIKWLDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQ 212
            SQ W  +  + P+VA+I     +CL++  GEN+ V + +C      Q+WAL+GD TIR 
Sbjct: 141 ASQGWTVSNDVKPIVASIVGYKEMCLQAN-GENNGVWMEDCEVTSLQQQWALFGDRTIRV 200

Query: 213 NEYRHLCLTSSERNYGALVVVSRCADKPQQRWGLAEDNTINHPNTNLVMDVHREVPLLPP 272
           N  R LC+TS+  N   L++  +C   P QRW    +  I +PN+ LVMDV      L  
Sbjct: 201 NSSRGLCVTSNGYNSKDLIITLKCQGLPSQRWFFNSNGAIVNPNSTLVMDVRASNVSLRE 260

Query: 273 MIVVDKHDGAPSQRW 283
            I++    G P+Q+W
Sbjct: 261 -IIISPATGNPNQQW 266

[Close]

BLAST of CmaCh04G005230 vs. NCBI nr
Match: gi|449457105|ref|XP_004146289.1| (PREDICTED: nigrin b-like [Cucumis sativus])

HSP 1 Score: 280.4 bits (716), Expect = 3.5e-72
Identity = 157/296 (53.04%), Postives = 201/296 (67.91%), Query Frame = 1


Query: 4   MRQVLLCIALAFSL----AIAAPSEYG--AFGGRTHLVGRDGLCLDTM--LLSGYYPQTD 63
           M++++L I +AFSL    A+A PS+YG     G THLVGRDGLCL+       G    T 
Sbjct: 1   MKEIVLSIVVAFSLTTHLAMAVPSDYGYQVVPGATHLVGRDGLCLEMSPWFYKGSNFPTR 60

Query: 64  LSQC-QPRKLSQLWTILEDGTIRASDVRYCLVP--KTQFIGPVGPNVVVMDCTKEVRHDM 123
           LS C + +K +QLWT+L+D TIR  + RYCLV      FI     NVVV +C KE   + 
Sbjct: 61  LSPCNEQKKQTQLWTVLQDETIRPMNDRYCLVSYVSVNFI----TNVVVSECAKEPHSNK 120

Query: 124 KWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKWLDNLC 183
           KW  K+D TI H  SG+VLT      VT++ N++APSQSWEAT+SL PMVANIKWL+NLC
Sbjct: 121 KWIHKEDRTIVHVGSGMVLTGN-SNYVTVQRNKNAPSQSWEATKSLTPMVANIKWLENLC 180

Query: 184 LKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVVVSRCA 243
           L+S +  N YV L+ C+ ++K+Q W+LYGDGTIR++  R+ CLT SE+++G  V VS+C 
Sbjct: 181 LQSTKDSN-YVQLDGCNSENKSQHWSLYGDGTIRKHVNRNYCLT-SEQDFGRFVAVSKCE 240

Query: 244 DKPQQRWGL-AEDNTINHPNTNLVMDVHREVPL--LPPMIVVDKHDGAPSQRWSIY 286
           DKPQQRWGL AEDNTINHPNT++VMDV   VP   LP  +V +  DG  +QRW IY
Sbjct: 241 DKPQQRWGLGAEDNTINHPNTDMVMDV-LSVPFDDLPLAVVTNHRDGTATQRWIIY 288

[Close]

BLAST of CmaCh04G005230 vs. NCBI nr
Match: gi|659112133|ref|XP_008456079.1| (PREDICTED: nigrin b-like [Cucumis melo])

HSP 1 Score: 269.2 bits (687), Expect = 8.0e-69
Identity = 151/297 (50.84%), Postives = 202/297 (68.01%), Query Frame = 1


Query: 4   MRQVLLCIALAFSL----AIAAPSEYG---AFGG---RTHLVGRDGLCLDTM--LLSGYY 63
           M++++L I +AFSL    A+A PS YG    +GG    THLVGRDGLCL+       G  
Sbjct: 1   MKEIVLSIIVAFSLTTHLAMAVPSNYGYGGEYGGVPRATHLVGRDGLCLEMSPWFYRGNN 60

Query: 64  PQTDLSQC-QPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKEVRH 123
             T LS C + +K +QLWT++ DGTIR  + ++CLV     +G +    VV +C KE   
Sbjct: 61  FPTRLSPCDEHKKQTQLWTVVGDGTIRPMNDKFCLVADV-LVGLIN-KAVVRECAKEPNS 120

Query: 124 DMKWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKWLDN 183
           + +WT KKDGTI H ESG+VLT      VTL++N++ PSQSWEATES  PM+ANI+WL+N
Sbjct: 121 NKRWTHKKDGTITHVESGMVLTGDSD-YVTLQSNKNTPSQSWEATESSTPMIANIEWLNN 180

Query: 184 LCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVVVSR 243
           LCL+S +  N +V LN C+R++K+QRWALYGDGTIRQN  R+ CLTS E   G  +V+S+
Sbjct: 181 LCLQSTDDSN-HVGLNGCNRENKDQRWALYGDGTIRQNVNRNYCLTSDE-YLGRFIVMSK 240

Query: 244 CADKPQQRWGL-AEDNTINHPNTNLVMDVHREVPL--LPPMIVVDKHDGAPSQRWSI 285
           C DKPQQRW L A+D+TI+HPN+++VMDV   VP       +V ++ DG+ SQRW+I
Sbjct: 241 CEDKPQQRWNLGAKDSTIDHPNSDMVMDV-LFVPYYDFSSAVVTNRRDGSASQRWTI 291

[Close]

BLAST of CmaCh04G005230 vs. NCBI nr
Match: gi|778680239|ref|XP_004151509.2| (PREDICTED: nigrin b-like [Cucumis sativus])

HSP 1 Score: 260.4 bits (664), Expect = 3.7e-66
Identity = 148/298 (49.66%), Postives = 193/298 (64.77%), Query Frame = 1


Query: 4   MRQVLLCIALAFSLA----IAAPSEYG---AFGG---RTHLVGRDGLCLDTMLLSGYYP- 63
           M++++L I +AFSL     IA P  YG    +GG    THLVGRDGLCL+ +LL  Y P 
Sbjct: 1   MKEIVLSIIVAFSLTTQLVIAVPPNYGYGVGYGGVPGATHLVGRDGLCLEMVLLRSYKPA 60

Query: 64  ----QTDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKEV 123
                T L  C  +K +QLWT++ DGTIR  + R+CL     F G +    VV +C K  
Sbjct: 61  GINFPTRLLPCDEKKQTQLWTVVGDGTIRPMNDRFCLAADV-FYGVIN-KAVVSECGKVS 120

Query: 124 RHDMKWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKWL 183
             + +W  K DGTI   ES +VLT      VTL++ +  PSQSWE TESL PMVANI+WL
Sbjct: 121 DSNKQWIHKNDGTIALAESRMVLTG-DSDYVTLQSIKYTPSQSWEVTESLNPMVANIEWL 180

Query: 184 DNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVVV 243
           +NLCL+S + ++S V LN C+   K QRWALY DGTIRQ+  R+ CLT SE+NY   VVV
Sbjct: 181 NNLCLQSTD-DSSNVGLNGCNTYTKYQRWALYADGTIRQHVNRNYCLT-SEQNYDRSVVV 240

Query: 244 SRCADKPQQRWGL-AEDNTINHPNTNLVMDVHREVPLLPPMIVVDKH-DGAPSQRWSI 285
           S+C DKPQQRW L A+D+TI+HPNTN+V+DV   +    P+++V  H DG+ SQRW++
Sbjct: 241 SKCEDKPQQRWSLNAKDSTIDHPNTNMVLDVLNVLDSASPLVIVTNHPDGSASQRWTM 293

[Close]

BLAST of CmaCh04G005230 vs. NCBI nr
Match: gi|566006087|ref|NP_001274403.1| (uncharacterized protein LOC101221335 precursor [Cucumis sativus])

HSP 1 Score: 256.9 bits (655), Expect = 4.1e-65
Identity = 148/300 (49.33%), Postives = 201/300 (67.00%), Query Frame = 1


Query: 4   MRQVLLCIALAFSL----AIAAPSEYG---AFGG---RTHLVGRDGLCLDTMLLSGYYPQ 63
           M++++L I +AFSL    AIA P  YG    +GG    THLVGRDGLCL+   +S +Y  
Sbjct: 1   MKEIVLSIIVAFSLTTQLAIAVPPNYGYGVGYGGVPGATHLVGRDGLCLE---MSPWYKP 60

Query: 64  ------TDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKE 123
                 T LS C  +K +QLWTI+ DGTIR  + ++CL  +  F G +    VV +C K 
Sbjct: 61  AGINFPTRLSPCDEKKQTQLWTIVGDGTIRPMNDKFCLAAEV-FYGVIN-KAVVSECGKV 120

Query: 124 VRHDMKWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKW 183
              + KWT+K DGTI   +S +VLT      VTL++N+  PSQSWE TESL  MVANI+W
Sbjct: 121 SDPNKKWTQKNDGTIALVDSRMVLTGDLD-YVTLQSNKYTPSQSWEVTESLNSMVANIEW 180

Query: 184 LDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVV 243
           L+NLCL+S + ++S+V LN C+ D+K QRWALY DGTIRQ+  ++ CLT S++++G  VV
Sbjct: 181 LNNLCLQSTD-DSSHVGLNGCNTDNKYQRWALYADGTIRQHVNKNYCLT-SDQDFGRFVV 240

Query: 244 VSRCADKPQQRWGL-AEDNTINHPNTNLVMDVHREVP--LLPPMIVVDKHDGAPSQRWSI 285
           VS+C DKPQQRW L A+D TI+HPNT++V+DV   VP    P ++V ++ DG+ SQRW+I
Sbjct: 241 VSKCEDKPQQRWSLDAKDYTIDHPNTDMVLDVF-SVPDSTFPSVLVTNRRDGSASQRWTI 291

[Close]

BLAST of CmaCh04G005230 vs. NCBI nr
Match: gi|700202446|gb|KGN57579.1| (hypothetical protein Csa_3G217160 [Cucumis sativus])

HSP 1 Score: 255.4 bits (651), Expect = 1.2e-64
Identity = 147/300 (49.00%), Postives = 200/300 (66.67%), Query Frame = 1


Query: 4   MRQVLLCIALAFSLA----IAAPSEYG---AFGG---RTHLVGRDGLCLDTMLLSGYYPQ 63
           M++++L I +AFSL     IA P  YG    +GG    THLVGRDGLCL+   +S +Y  
Sbjct: 1   MKEIVLSIIVAFSLTTQLVIAVPPNYGYGVGYGGVPGATHLVGRDGLCLE---MSPWYKP 60

Query: 64  ------TDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKE 123
                 T LS C  +K +QLWTI+ DGTIR  + ++CL  +  F G +    VV +C K 
Sbjct: 61  AGINFPTRLSPCDEKKQTQLWTIVGDGTIRPMNDKFCLAAEV-FYGVIN-KAVVSECGKV 120

Query: 124 VRHDMKWTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKW 183
              + KWT+K DGTI   +S +VLT      VTL++N+  PSQSWE TESL  MVANI+W
Sbjct: 121 SDPNKKWTQKNDGTIALVDSRMVLTGDLD-YVTLQSNKYTPSQSWEVTESLNSMVANIEW 180

Query: 184 LDNLCLKSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVV 243
           L+NLCL+S + ++S+V LN C+ D+K QRWALY DGTIRQ+  ++ CLT S++++G  VV
Sbjct: 181 LNNLCLQSTD-DSSHVGLNGCNTDNKYQRWALYADGTIRQHVNKNYCLT-SDQDFGRFVV 240

Query: 244 VSRCADKPQQRWGL-AEDNTINHPNTNLVMDVHREVP--LLPPMIVVDKHDGAPSQRWSI 285
           VS+C DKPQQRW L A+D TI+HPNT++V+DV   VP    P ++V ++ DG+ SQRW+I
Sbjct: 241 VSKCEDKPQQRWSLDAKDYTIDHPNTDMVLDVF-SVPDSTFPSVLVTNRRDGSASQRWTI 291

The following BLAST results are available for this feature:

BLAST of CmaCh04G005230 vs. Swiss-Prot


Match Name	E-value	Identity	Description
NIGB_SAMNI	2.2e-34	35.69	Nigrin b OS=Sambucus nigra PE=1 SV=2	[more]
ABRC_ABRPR	1.1e-30	34.25	Abrin-c OS=Abrus precatorius PE=1 SV=1	[more]
SGSL_TRIAN	4.3e-30	32.44	Seed lectin (Fragments) OS=Trichosanthes anguina PE=1 SV=2	[more]
RIP1_SAMNI	7.3e-30	34.24	Ribosome-inactivating protein SNAI' OS=Sambucus nigra PE=1 SV=1	[more]
ABRB_ABRPR	1.2e-29	33.86	Abrin-b OS=Abrus precatorius PE=1 SV=1	[more]

BLAST of CmaCh04G005230 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L756_CUCSA	2.4e-72	53.04	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G219180 PE=4 SV=1	[more]
A0A0A0LAC2_CUCSA	2.6e-66	49.66	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217140 PE=4 SV=1	[more]
Q9SLV9_CUCSA	2.9e-65	49.33	XSP30 OS=Cucumis sativus GN=XSP30 PE=2 SV=1	[more]
A0A0A0L9D1_CUCSA	8.3e-65	49.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217160 PE=4 SV=1	[more]
Q58A89_SAMRA	9.9e-34	35.69	Lectin OS=Sambucus racemosa subsp. sieboldiana GN=LecSSA-b-3a PE=2 SV=1	[more]

BLAST of CmaCh04G005230 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CmaCh04G005230 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449457105\|ref\|XP_004146289.1\|	3.5e-72	53.04	PREDICTED: nigrin b-like [Cucumis sativus]	[more]
gi\|659112133\|ref\|XP_008456079.1\|	8.0e-69	50.84	PREDICTED: nigrin b-like [Cucumis melo]	[more]
gi\|778680239\|ref\|XP_004151509.2\|	3.7e-66	49.66	PREDICTED: nigrin b-like [Cucumis sativus]	[more]
gi\|566006087\|ref\|NP_001274403.1\|	4.1e-65	49.33	uncharacterized protein LOC101221335 precursor [Cucumis sativus]	[more]
gi\|700202446\|gb\|KGN57579.1\|	1.2e-64	49.00	hypothetical protein Csa_3G217160 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000772	Ricin_B_lectin

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G005230.1	CmaCh04G005230.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000772	Ricin B, lectin domain	PFAM	PF00652	Ricin_B_lectin	coord: 169..282 score: 5.6E-22coord: 33..152 score: 1.5
IPR000772	Ricin B, lectin domain	SMART	SM00458	ricin_3	coord: 29..155 score: 5.3E-4coord: 159..285 score: 9.6
IPR000772	Ricin B, lectin domain	PROFILE	PS50231	RICIN_B_LECTIN	coord: 31..155 score: 21.382coord: 169..285 score: 27
IPR000772	Ricin B, lectin domain	unknown	SSF50370	Ricin B-like lectins	coord: 32..153 score: 1.91E-16coord: 159..284 score: 1.2
None	No IPR available	GENE3D	G3DSA:2.80.10.50		coord: 33..153 score: 2.8E-15coord: 164..284 score: 5.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh04G005230	Lsi05G001810	Bottle gourd (USVL1VR-Ls)	cmalsiB690

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh04G005230	Cucumber (Gy14) v2	cgybcmaB410
CmaCh04G005230	Melon (DHL92) v3.6.1	cmamedB777
CmaCh04G005230	Silver-seed gourd	carcmaB0086
CmaCh04G005230	Silver-seed gourd	carcmaB0925
CmaCh04G005230	Cucumber (Chinese Long) v3	cmacucB0829
CmaCh04G005230	Watermelon (97103) v2	cmawmbB763
CmaCh04G005230	Wax gourd	cmawgoB0852
CmaCh04G005230	Cucurbita maxima (Rimu)	cmacmaB351
CmaCh04G005230	Cucumber (Gy14) v1	cgycmaB0012
CmaCh04G005230	Cucurbita moschata (Rifu)	cmacmoB701
CmaCh04G005230	Cucurbita moschata (Rifu)	cmacmoB728
CmaCh04G005230	Wild cucumber (PI 183967)	cmacpiB705
CmaCh04G005230	Cucumber (Chinese Long) v2	cmacuB697
CmaCh04G005230	Melon (DHL92) v3.5.1	cmameB691
CmaCh04G005230	Watermelon (Charleston Gray)	cmawcgB659
CmaCh04G005230	Watermelon (97103) v1	cmawmB703
CmaCh04G005230	Cucurbita pepo (Zucchini)	cmacpeB715
CmaCh04G005230	Cucurbita pepo (Zucchini)	cmacpeB720