Cucsa.282590 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.282590
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Iron-sulfur cluster assembly protein, putative
Location	scaffold02653 : 30098 .. 32590 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGAAAACACTCGAAAGTTGTAATCTGCGGAGGAGAAAATTGATCGATAGGCAACAAGAGGTGAGAAAATGGCAGCTTCTTCGTCTTCCTTTTTCGCCGCCGCTTCTCAGAAGGCCGGACCAGCCGCTCGCCGCCAAATTCTTACTCTCACGGAGGCAGCCGCCGCTAGAATTCGGCAACTTCTTCAACAGCGCCAACGTCCGTTCCTTCGTCTTGGCGTCAAGGCGCGCGGTTGTAATGGTTTATCCTATACTCTCAACTACGCAGGTCCGTAATAGTCTAATGAATTTCAACTTTTTATTGATTCTACGTAATTTCTCTTTTACCCATGTGATTGAATTTGTCGATTTTTTATCTCTTTTCTTTGAATCAGATTTCGATTTTGGATTTTATTGGAGATAAATAGGTGTTTGGTGTATGTGAGCGATACAACCTGATAGTTTAGACGAGATGTTTCGGAACTAGTCTTTTAGAAGATGTTTTTTTTTTATCTTTGTACTGTTTCCAGCTCCGATTACCAAAATTAGGATTTTAAAACCGATGATCTATTATTATGATTAGACGTAAAAAATTAAGAAGGTCAAATGATTCTGTAATTGTTATAACCGGAATTAGGCATGGTATGGTATGTAAGTTGTTCGGAAATGAGCTGTTGAGTTCTTGCTAGGCTTAACAACGGCAAATCTGAACGATAAAACTTGATGTTTCTGAATGCATAAGTTGATAAACTATCGATGATTTTACATACAGGGCCAGGGACCTATCTAACTACTTCAGTCTGTAAATCATTCTGTATCTTATAGAATCAGTTATGAATTATTGGTGCTATGGGACAGAAAGTTTGGATTCCATGATTTCCTTCTCCAACCTATGGCACAGGGATTATTGTGTTATTGATATATCAAAATGTCAAACTAAATGCACGGTCCACCAAAATTTTGTGGTGAACATTTGCTTCCATCAAGCACCATTTAGATTGTGATGTATGTTGGCTATTGTGAGAACATAGCAGGATTGGATTGGGTATCTATGGTTAATTATAATGTTATTAGACCAAAGTGCTATTTCTGTTCACTTAGAAGATAAATGGGTTAGCAATTCTCCATGGGTCAGCTTGAAGAGAATAGTTATTAACATGTTTGGTGGTTGAAATTTGAGATTTTATGCTCTTTGTGGCTTGGGATTCTTTTATTCCTTGTGCTTATTTAATATGACTGTCTTCTCAGTTAACAGTATTTACAATTTAATTTTGTATGGAGTTGCTCATTATTGGAGAGTTTTCTAACTTGGCATTACCTCAAATTTTTTTGTTATCGCATTATCGTTAACATTTTCTTTATGCAAGAAACAGGATTCATCTTTGATCTCCGTTTGGCATAAATTGATTATAGTTACGATCTCTATCTTTCAAAATACTTTAAGATTGCAGTATTTCATTTAACAGTTTCCTTTTGCAAATATTTCAGATGAGAAAGGGAAGTTTGATGAGCTGGTTGAGGATCAAAATGTGAAAATACTGATTGAACCAAAGGCTCTAATGCACGTAATAGGAACAAAGATGGATTTTGTCGATGACAAATTAAGGTATTTGATAAATCTCTTTCCTAGAATTTAATGTATTCAGCTCTATATATCCATCCTTAAGTTGATGAAATATCCTGCTTGTTACAAGAGTTAGTGATGTAATTGCTCTAGTTAGGATTTATTGTGGGTTGTATTAAGTCCATCATAAATACAGAATTCAATGTTAGTTCTCAAGGTTAATTAATTCTTTTAATTAGTTCTCAAGCTCGAGAGTTGATTTAGATCATCTTTAACACATCCACAAGCTGGTGAATATAATTAATACAGCTTAAGAGAGAACTGTTAAGTTTATTAGATTCAGTTCATCTGACTATAGCTCAGTGGGTTTGATCCTCTACCTTGCAATGGATGGACAAAAAAAGGGTTGATTAGATTCGGGTTATTTTGAGTCTCTATATCAATTCAAATTCAAATTCAAATTCAATCTTTTAACCTCTTGTATTCAGTGGGCATTGAAACTTATTTTTCTCTCTCTTTTCTCTGGTTGGTAGATCTGAGTTTATCTTTATCAATCCAAATTCCAAAGGTCAGTGTGGCTGTGGGGAGTCCTTTATGACAACAAGTAGTGCAGAAGCCAACAAAAAATGAGGCAGTTCGAGCAATGAGCGTTGTTTTTAAGAACTCAAAACTCACAGTTGCTGATTTGTTGTGTATTTTGCTGGAATTACAAAGACAATGATCAAGAGAGTTCTCTGACAACAACTTTAAACATTTGTAAGAATATTTCTATATTGGCTGATTATATTCTTAGAAAGCCTGTTAAGAGTTGTACTCTGCTAATTTTTCTTTCTTTAAACATATTTTGTATGTATATTACTGTAGATTAAACTGGCTGATGAGAGCCTATGAGGTAATAAAATTCGTCCACGACTTTCTTTTGTTAAAGTCATAGAACTTGGTAACTAAAAAA

mRNA sequence

GGAAAACACTCGAAAGTTGTAATCTGCGGAGGAGAAAATTGATCGATAGGCAACAAGAGGTGAGAAAATGGCAGCTTCTTCGTCTTCCTTTTTCGCCGCCGCTTCTCAGAAGGCCGGACCAGCCGCTCGCCGCCAAATTCTTACTCTCACGGAGGCAGCCGCCGCTAGAATTCGGCAACTTCTTCAACAGCGCCAACGTCCGTTCCTTCGTCTTGGCGTCAAGGCGCGCGGTTGTAATGGTTTATCCTATACTCTCAACTACGCAGATGAGAAAGGGAAGTTTGATGAGCTGGTTGAGGATCAAAATGTGAAAATACTGATTGAACCAAAGGCTCTAATGCACGTAATAGGAACAAAGATGGATTTTGTCGATGACAAATTAAGATCTGAGTTTATCTTTATCAATCCAAATTCCAAAGGTCAGTGTGGCTGTGGGGAGTCCTTTATGACAACAAGTAGTGCAGAAGCCAACAAAAAATGAGGCAGTTCGAGCAATGAGCGTTGTTTTTAAGAACTCAAAACTCACAGTTGCTGATTTGTTGTGTATTTTGCTGGAATTACAAAGACAATGATCAAGAGAGTTCTCTGACAACAACTTTAAACATTTGTAAGAATATTTCTATATTGGCTGATTATATTCTTAGAAAGCCTGTTAAGAGTTGTACTCTGCTAATTTTTCTTTCTTTAAACATATTTTGTATGTATATTACTGTAGATTAAACTGGCTGATGAGAGCCTATGAGGTAATAAAATTCGTCCACGACTTTCTTTTGTTAAAGTCATAGAACTTGGTAACTAAAAAA

Coding sequence (CDS)

ATGGCAGCTTCTTCGTCTTCCTTTTTCGCCGCCGCTTCTCAGAAGGCCGGACCAGCCGCTCGCCGCCAAATTCTTACTCTCACGGAGGCAGCCGCCGCTAGAATTCGGCAACTTCTTCAACAGCGCCAACGTCCGTTCCTTCGTCTTGGCGTCAAGGCGCGCGGTTGTAATGGTTTATCCTATACTCTCAACTACGCAGATGAGAAAGGGAAGTTTGATGAGCTGGTTGAGGATCAAAATGTGAAAATACTGATTGAACCAAAGGCTCTAATGCACGTAATAGGAACAAAGATGGATTTTGTCGATGACAAATTAAGATCTGAGTTTATCTTTATCAATCCAAATTCCAAAGGTCAGTGTGGCTGTGGGGAGTCCTTTATGACAACAAGTAGTGCAGAAGCCAACAAAAAATGA

Protein sequence

MAASSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTSSAEANKK*

Homology

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BLAST of Cucsa.282590 vs. Swiss-Prot
Match: ISAM1_ARATH (Iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Arabidopsis thaliana GN=At2g16710 PE=2 SV=2)

HSP 1 Score: 218.0 bits (554), Expect = 6.4e-56
Identity = 102/132 (77.27%), Postives = 120/132 (90.91%), Query Frame = 1


Query: 5   SSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLN 64
           +S   AAA+ + GPA R+Q+LTLT+ AA+R+  LLQQRQ+PFLRLGVKARGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGE 124
           YADEKGKFDELVE++ V+IL+EPKALMHVIGTKMDFVDDKLRSEF+FINPNS+GQCGCGE
Sbjct: 63  YADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVFINPNSQGQCGCGE 122

Query: 125 SFMTTSSAEANK 137
           SFMTTS++ A +
Sbjct: 123 SFMTTSTSSAKQ 134

[Close]

BLAST of Cucsa.282590 vs. Swiss-Prot
Match: ISAM3_ARATH (Iron-sulfur assembly protein IscA-like 3, mitochondrial OS=Arabidopsis thaliana GN=At2g36260 PE=3 SV=2)

HSP 1 Score: 172.9 bits (437), Expect = 2.4e-42
Identity = 80/109 (73.39%), Postives = 99/109 (90.83%), Query Frame = 1


Query: 21  RRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQN 80
           R+Q+L L++ AAARIRQLLQ +Q+PFLRL V+A+GCNGLSY LNYA EKGKFDE+VE++ 
Sbjct: 2   RKQVLALSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKG 61

Query: 81  VKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTT 130
           VKIL++PKA+MHVIGT+MDFVDDKLRSEF+F+NPN+  +CGCGESF TT
Sbjct: 62  VKILVDPKAVMHVIGTEMDFVDDKLRSEFVFVNPNAT-KCGCGESFTTT 109

[Close]

BLAST of Cucsa.282590 vs. Swiss-Prot
Match: ISA1_YEAST (Iron-sulfur assembly protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ISA1 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 5.7e-28
Identity = 57/107 (53.27%), Postives = 78/107 (72.90%), Query Frame = 1


Query: 21  RRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQN 80
           R+ ++TL+  A   +R LL Q +   +R+  + RGC+GL+Y L Y  E GKFDE+VE   
Sbjct: 143 RKALITLSPKAIKHLRALLAQPEPKLIRVSARNRGCSGLTYDLQYITEPGKFDEVVEQDG 202

Query: 81  VKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFM 128
           VKI+I+ KAL  +IG++MD++DDKL S+F+F NPNSKG CGCGESFM
Sbjct: 203 VKIVIDSKALFSIIGSEMDWIDDKLASKFVFKNPNSKGTCGCGESFM 249

[Close]

BLAST of Cucsa.282590 vs. Swiss-Prot
Match: ISCA1_DROME (Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Drosophila melanogaster GN=l(1)G0136 PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 4.8e-27
Identity = 61/106 (57.55%), Postives = 76/106 (71.70%), Query Frame = 1


Query: 22  RQILTLTEAAAARIRQLLQQRQRPF-LRLGVKARGCNGLSYTLNYADEKGKFDELVEDQN 81
           R  LTLT AA  RI+ LLQ +     L++GV+ RGCNGLSYTL+YA +K K DE V    
Sbjct: 23  RAALTLTPAAVLRIKTLLQDKPDMVGLKVGVRQRGCNGLSYTLDYASQKDKLDEEVVQDG 82

Query: 82  VKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESF 127
           VK+ I+ KA + ++GT+MDFV+ KL SEF+F NPN KG CGCGESF
Sbjct: 83  VKVFIDKKAQLSLLGTEMDFVESKLSSEFVFNNPNIKGTCGCGESF 128

[Close]

BLAST of Cucsa.282590 vs. Swiss-Prot
Match: ISCA1_COLLI (Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Columba livia GN=ISCA1 PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 4.8e-27
Identity = 65/130 (50.00%), Postives = 87/130 (66.92%), Query Frame = 1


Query: 1   MAASSSSFFAA---ASQKAGPAARRQILTLTEAAAARIRQLLQQR-QRPFLRLGVKARGC 60
           MA+S+SS   A   A  K    A R  LTLT +A  +I++LL+ + +   +++GV+ RGC
Sbjct: 1   MASSASSVVRATVRAVSKRKIQATRAALTLTPSAVQKIKELLKDKPEHVGVKVGVRTRGC 60

Query: 61  NGLSYTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNS 120
           NGLSYTL Y   KG  DE V    V++ IE KA + ++GT+MD+V+DKL SEF+F NPN 
Sbjct: 61  NGLSYTLEYTKSKGDSDEEVVQDGVRVFIEKKAQLTLLGTEMDYVEDKLSSEFVFNNPNI 120

Query: 121 KGQCGCGESF 127
           KG CGCGESF
Sbjct: 121 KGTCGCGESF 130

[Close]

BLAST of Cucsa.282590 vs. TrEMBL
Match: A0A0A0KTF0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G614650 PE=4 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 1.6e-69
Identity = 137/137 (100.00%), Postives = 137/137 (100.00%), Query Frame = 1


Query: 1   MAASSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTSSAEANKK 138
           GCGESFMTTSSAEANKK
Sbjct: 121 GCGESFMTTSSAEANKK 137

[Close]

BLAST of Cucsa.282590 vs. TrEMBL
Match: A0A059AA55_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_J00259 PE=4 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 6.2e-58
Identity = 113/137 (82.48%), Postives = 127/137 (92.70%), Query Frame = 1


Query: 1   MAASSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAA S+S  AAA+ + G AARRQ+LT+T+AAA+RIR LLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAAPSASKLAAAADRFGSAARRQVLTVTDAAASRIRHLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYAD KGKFDELVED+ +KIL+EPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADSKGKFDELVEDKGIKILVEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTSSAEANKK 138
           GCGESFMTTS+++A K+
Sbjct: 121 GCGESFMTTSNSDAAKR 137

[Close]

BLAST of Cucsa.282590 vs. TrEMBL
Match: B8R1J7_EUCGR (Iron-sulfer cluster scaffold protein ISA1 (Fragment) OS=Eucalyptus grandis GN=ISA1 PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 6.2e-58
Identity = 113/137 (82.48%), Postives = 127/137 (92.70%), Query Frame = 1


Query: 1   MAASSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAA S+S  AAA+ + G AARRQ+LT+T+AAA+RIR LLQQRQRPFLRLGVKARGCNGLS
Sbjct: 28  MAAPSASKLAAAADRFGSAARRQVLTVTDAAASRIRHLLQQRQRPFLRLGVKARGCNGLS 87

Query: 61  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYAD KGKFDELVED+ +KIL+EPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 88  YTLNYADSKGKFDELVEDKGIKILVEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 147

Query: 121 GCGESFMTTSSAEANKK 138
           GCGESFMTTS+++A K+
Sbjct: 148 GCGESFMTTSNSDAAKR 164

[Close]

BLAST of Cucsa.282590 vs. TrEMBL
Match: A0A103Y9P2_CYNCS (FeS cluster biogenesis OS=Cynara cardunculus var. scolymus GN=Ccrd_016577 PE=4 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 1.8e-57
Identity = 113/133 (84.96%), Postives = 123/133 (92.48%), Query Frame = 1


Query: 4   SSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTL 63
           ++S   AAA+++ G AARRQ LTLTEAAA RIR LLQQRQRPFL+LGVKARGCNGLSYTL
Sbjct: 37  ATSKLAAAAAERLGTAARRQALTLTEAAAGRIRHLLQQRQRPFLKLGVKARGCNGLSYTL 96

Query: 64  NYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCG 123
           NYADEKGKFDEL+ED+ VKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCG
Sbjct: 97  NYADEKGKFDELIEDKGVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCG 156

Query: 124 ESFMTTSSAEANK 137
           ESFMTTS+AEA K
Sbjct: 157 ESFMTTSTAEATK 169

[Close]

BLAST of Cucsa.282590 vs. TrEMBL
Match: A0A0K9QLU5_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_164410 PE=4 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 5.3e-57
Identity = 111/132 (84.09%), Postives = 124/132 (93.94%), Query Frame = 1


Query: 6   SSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNY 65
           ++F A A++KAGP  RRQ L+L++AAA RI+QLLQQRQR FLRLGVKARGCNGLSYTLNY
Sbjct: 2   AAFLAKAAEKAGPTIRRQALSLSDAAATRIQQLLQQRQRSFLRLGVKARGCNGLSYTLNY 61

Query: 66  ADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGES 125
           ADEKGKFDEL+ED+ VKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGES
Sbjct: 62  ADEKGKFDELIEDKGVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGES 121

Query: 126 FMTTSSAEANKK 138
           FMTTSS+EA+K+
Sbjct: 122 FMTTSSSEASKQ 133

[Close]

BLAST of Cucsa.282590 vs. TAIR10
Match: AT2G16710.3 (AT2G16710.3 Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 211.5 bits (537), Expect = 3.4e-55
Identity = 102/138 (73.91%), Postives = 120/138 (86.96%), Query Frame = 1


Query: 5   SSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLN 64
           +S   AAA+ + GPA R+Q+LTLT+ AA+R+  LLQQRQ+PFLRLGVKARGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YA------DEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKG 124
           YA      DEKGKFDELVE++ V+IL+EPKALMHVIGTKMDFVDDKLRSEF+FINPNS+G
Sbjct: 63  YAGKTSGNDEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVFINPNSQG 122

Query: 125 QCGCGESFMTTSSAEANK 137
           QCGCGESFMTTS++ A +
Sbjct: 123 QCGCGESFMTTSTSSAKQ 140

[Close]

BLAST of Cucsa.282590 vs. TAIR10
Match: AT2G36260.1 (AT2G36260.1 Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 172.9 bits (437), Expect = 1.3e-43
Identity = 80/109 (73.39%), Postives = 99/109 (90.83%), Query Frame = 1


Query: 21  RRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQN 80
           R+Q+L L++ AAARIRQLLQ +Q+PFLRL V+A+GCNGLSY LNYA EKGKFDE+VE++ 
Sbjct: 2   RKQVLALSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKG 61

Query: 81  VKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTT 130
           VKIL++PKA+MHVIGT+MDFVDDKLRSEF+F+NPN+  +CGCGESF TT
Sbjct: 62  VKILVDPKAVMHVIGTEMDFVDDKLRSEFVFVNPNAT-KCGCGESFTTT 109

[Close]

BLAST of Cucsa.282590 vs. NCBI nr
Match: gi|449434502|ref|XP_004135035.1| (PREDICTED: iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucumis sativus])

HSP 1 Score: 270.0 bits (689), Expect = 2.3e-69
Identity = 137/137 (100.00%), Postives = 137/137 (100.00%), Query Frame = 1


Query: 1   MAASSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTSSAEANKK 138
           GCGESFMTTSSAEANKK
Sbjct: 121 GCGESFMTTSSAEANKK 137

[Close]

BLAST of Cucsa.282590 vs. NCBI nr
Match: gi|659091836|ref|XP_008446757.1| (PREDICTED: iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucumis melo])

HSP 1 Score: 267.3 bits (682), Expect = 1.5e-68
Identity = 135/137 (98.54%), Postives = 136/137 (99.27%), Query Frame = 1


Query: 1   MAASSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAASQKAGPA RRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASQKAGPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQNVKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTSSAEANKK 138
           GCGESFMTTSSAEANKK
Sbjct: 121 GCGESFMTTSSAEANKK 137

[Close]

BLAST of Cucsa.282590 vs. NCBI nr
Match: gi|702473214|ref|XP_010031271.1| (PREDICTED: iron-sulfur assembly protein IscA-like 1, mitochondrial [Eucalyptus grandis])

HSP 1 Score: 231.5 bits (589), Expect = 9.0e-58
Identity = 113/137 (82.48%), Postives = 127/137 (92.70%), Query Frame = 1


Query: 1   MAASSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAA S+S  AAA+ + G AARRQ+LT+T+AAA+RIR LLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAAPSASKLAAAADRFGSAARRQVLTVTDAAASRIRHLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYAD KGKFDELVED+ +KIL+EPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADSKGKFDELVEDKGIKILVEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTSSAEANKK 138
           GCGESFMTTS+++A K+
Sbjct: 121 GCGESFMTTSNSDAAKR 137

[Close]

BLAST of Cucsa.282590 vs. NCBI nr
Match: gi|209484093|gb|ACI47521.1| (iron-sulfer cluster scaffold protein ISA1, partial [Eucalyptus grandis])

HSP 1 Score: 231.5 bits (589), Expect = 9.0e-58
Identity = 113/137 (82.48%), Postives = 127/137 (92.70%), Query Frame = 1


Query: 1   MAASSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAA S+S  AAA+ + G AARRQ+LT+T+AAA+RIR LLQQRQRPFLRLGVKARGCNGLS
Sbjct: 28  MAAPSASKLAAAADRFGSAARRQVLTVTDAAASRIRHLLQQRQRPFLRLGVKARGCNGLS 87

Query: 61  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYAD KGKFDELVED+ +KIL+EPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 88  YTLNYADSKGKFDELVEDKGIKILVEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 147

Query: 121 GCGESFMTTSSAEANKK 138
           GCGESFMTTS+++A K+
Sbjct: 148 GCGESFMTTSNSDAAKR 164

[Close]

BLAST of Cucsa.282590 vs. NCBI nr
Match: gi|976920368|gb|KVI05099.1| (FeS cluster biogenesis [Cynara cardunculus var. scolymus])

HSP 1 Score: 229.9 bits (585), Expect = 2.6e-57
Identity = 113/133 (84.96%), Postives = 123/133 (92.48%), Query Frame = 1


Query: 4   SSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTL 63
           ++S   AAA+++ G AARRQ LTLTEAAA RIR LLQQRQRPFL+LGVKARGCNGLSYTL
Sbjct: 37  ATSKLAAAAAERLGTAARRQALTLTEAAAGRIRHLLQQRQRPFLKLGVKARGCNGLSYTL 96

Query: 64  NYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCG 123
           NYADEKGKFDEL+ED+ VKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCG
Sbjct: 97  NYADEKGKFDELIEDKGVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCG 156

Query: 124 ESFMTTSSAEANK 137
           ESFMTTS+AEA K
Sbjct: 157 ESFMTTSTAEATK 169

The following BLAST results are available for this feature:

BLAST of Cucsa.282590 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ISAM1_ARATH	6.4e-56	77.27	Iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Arabidopsis thaliana ...	[more]
ISAM3_ARATH	2.4e-42	73.39	Iron-sulfur assembly protein IscA-like 3, mitochondrial OS=Arabidopsis thaliana ...	[more]
ISA1_YEAST	5.7e-28	53.27	Iron-sulfur assembly protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 /...	[more]
ISCA1_DROME	4.8e-27	57.55	Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Drosophila melanogaster...	[more]
ISCA1_COLLI	4.8e-27	50.00	Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Columba livia GN=ISCA1 ...	[more]

BLAST of Cucsa.282590 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KTF0_CUCSA	1.6e-69	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G614650 PE=4 SV=1	[more]
A0A059AA55_EUCGR	6.2e-58	82.48	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_J00259 PE=4 SV=1	[more]
B8R1J7_EUCGR	6.2e-58	82.48	Iron-sulfer cluster scaffold protein ISA1 (Fragment) OS=Eucalyptus grandis GN=IS...	[more]
A0A103Y9P2_CYNCS	1.8e-57	84.96	FeS cluster biogenesis OS=Cynara cardunculus var. scolymus GN=Ccrd_016577 PE=4 S...	[more]
A0A0K9QLU5_SPIOL	5.3e-57	84.09	Uncharacterized protein OS=Spinacia oleracea GN=SOVF_164410 PE=4 SV=1	[more]

BLAST of Cucsa.282590 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G16710.3	3.4e-55	73.91	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G36260.1	1.3e-43	73.39	Iron-sulphur cluster biosynthesis family protein	[more]

BLAST of Cucsa.282590 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449434502\|ref\|XP_004135035.1\|	2.3e-69	100.00	PREDICTED: iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucumis sati...	[more]
gi\|659091836\|ref\|XP_008446757.1\|	1.5e-68	98.54	PREDICTED: iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucumis melo...	[more]
gi\|702473214\|ref\|XP_010031271.1\|	9.0e-58	82.48	PREDICTED: iron-sulfur assembly protein IscA-like 1, mitochondrial [Eucalyptus g...	[more]
gi\|209484093\|gb\|ACI47521.1\|	9.0e-58	82.48	iron-sulfer cluster scaffold protein ISA1, partial [Eucalyptus grandis]	[more]
gi\|976920368\|gb\|KVI05099.1\|	2.6e-57	84.96	FeS cluster biogenesis [Cynara cardunculus var. scolymus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000361	FeS_biogenesis
IPR016092	FeS_cluster_insertion
IPR017870	FeS_cluster_insertion_CS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005198	structural molecule activity
GO:0051536	iron-sulfur cluster binding

Vocabulary: **Biological Process**
Term	Definition
GO:0016226	iron-sulfur cluster assembly

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0097428	protein maturation by iron-sulfur cluster transfer
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0005198	structural molecule activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.282590.1	Cucsa.282590.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000361	FeS cluster biogenesis	GENE3D	G3DSA:2.60.300.12		coord: 24..117 score: 8.7
IPR000361	FeS cluster biogenesis	PFAM	PF01521	Fe-S_biosyn	coord: 25..124 score: 8.2
IPR000361	FeS cluster biogenesis	unknown	SSF89360	HesB-like domain	coord: 24..117 score: 6.67
IPR016092	FeS cluster insertion protein	TIGRFAMs	TIGR00049	TIGR00049	coord: 25..127 score: 1.4
IPR017870	FeS cluster insertion, C-terminal, conserved site	PROSITE	PS01152	HESB	coord: 109..126 scor
None	No IPR available	PANTHER	PTHR10072	IRON-SULFUR CLUSTER ASSEMBLY PROTEIN	coord: 15..128 score: 1.2
None	No IPR available	PANTHER	PTHR10072:SF51	SUBFAMILY NOT NAMED	coord: 15..128 score: 1.2

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.282590	Cla020489	Watermelon (97103) v1	cgywmB524
Cucsa.282590	Csa5G614650	Cucumber (Chinese Long) v2	cgycuB410
Cucsa.282590	MELO3C012350	Melon (DHL92) v3.5.1	cgymeB474
Cucsa.282590	ClCG05G021780	Watermelon (Charleston Gray)	cgywcgB498
Cucsa.282590	CSPI05G26340	Wild cucumber (PI 183967)	cgycpiB432
Cucsa.282590	CmaCh18G009490	Cucurbita maxima (Rimu)	cgycmaB0797
Cucsa.282590	CmoCh04G015070	Cucurbita moschata (Rifu)	cgycmoB0793
Cucsa.282590	Lsi04G001520	Bottle gourd (USVL1VR-Ls)	cgylsiB465
Cucsa.282590	Cp4.1LG01g14330	Cucurbita pepo (Zucchini)	cgycpeB0750
Cucsa.282590	Cp4.1LG09g03640	Cucurbita pepo (Zucchini)	cgycpeB0745
Cucsa.282590	MELO3C012350.2	Melon (DHL92) v3.6.1	cgymedB471
Cucsa.282590	CsaV3_5G035380	Cucumber (Chinese Long) v3	cgycucB441
Cucsa.282590	Cla97C05G103500	Watermelon (97103) v2	cgywmbB498
Cucsa.282590	Bhi07G000635	Wax gourd	cgywgoB583
Cucsa.282590	Carg01902	Silver-seed gourd	carcgyB0968
Cucsa.282590	Carg18993	Silver-seed gourd	carcgyB1166

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.282590	Cucurbita maxima (Rimu)	cgycmaB0801
Cucsa.282590	Cucurbita moschata (Rifu)	cgycmoB0789